CSPI04G24020 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G24020
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calcium-binding protein CML39-like
Location	Chr4: 21942722 .. 21943672 (-)
RNA-Seq Expression	CSPI04G24020
Synteny	CSPI04G24020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTCTTTTAAATCATATACTTAAATAATCACCGTCTTACTCTAGACGTCAAAATCCGATGACATTTCATCAACATACTAACGTGTTTTTGAATGTTTAAACTTCAAACTAAAAACAGAGTCAGCCGTTGAAATCCATCACAAAGCACAGTTCCTAAATTGTACGACACTCTAAAGCCGAATTGGATTCCAATTTTGTAGCCGCACCAATCCCAATACAGAACACAAAGATAACTCCGCATCTCAATTCTCTAATCACCGCCATTAATTTCCATTTTTCTCTATAAATTCCCCTATTCCCCCACTTTCCATTTTCAACAACAAATCAAATCAAATCCAACTCCCCAATTTCCCAAACCACAATCAACTACAGAACCATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGATTTTTATTTACTGCCCCGCCGCCTCCCACGACGGCTTGTTGATCATATCGTCGGTTTCTATAAACTTGTCCAATAAATGGCGGCTGCTTCTTATGTGTATTTTTCTTTTATTTTACTTTCGAACCTCTGTTGCCTTTAATTTATTGACAATATATATCTCAATCAGTTGTATTTTAATTGTATATATCTTCATAAATTTAAGTTCGTATGTGTTGTTATGTTTAGCTAATTACTCATTTTGTGTTCATCACACTTTCAAAAATTGATAGCACCATGGCAAG

mRNA sequence

AATTCTTTTAAATCATATACTTAAATAATCACCGTCTTACTCTAGACGTCAAAATCCGATGACATTTCATCAACATACTAACGTGTTTTTGAATGTTTAAACTTCAAACTAAAAACAGAGTCAGCCGTTGAAATCCATCACAAAGCACAGTTCCTAAATTGTACGACACTCTAAAGCCGAATTGGATTCCAATTTTGTAGCCGCACCAATCCCAATACAGAACACAAAGATAACTCCGCATCTCAATTCTCTAATCACCGCCATTAATTTCCATTTTTCTCTATAAATTCCCCTATTCCCCCACTTTCCATTTTCAACAACAAATCAAATCAAATCCAACTCCCCAATTTCCCAAACCACAATCAACTACAGAACCATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGATTTTTATTTACTGCCCCGCCGCCTCCCACGACGGCTTGTTGATCATATCGTCGGTTTCTATAAACTTGTCCAATAAATGGCGGCTGCTTCTTATGTGTATTTTTCTTTTATTTTACTTTCGAACCTCTGTTGCCTTTAATTTATTGACAATATATATCTCAATCAGTTGTATTTTAATTGTATATATCTTCATAAATTTAAGTTCGTATGTGTTGTTATGTTTAGCTAATTACTCATTTTGTGTTCATCACACTTTCAAAAATTGATAGCACCATGGCAAG

Coding sequence (CDS)

ATGTCGTCGATGTTCCGTCCAAAAACGAAATCGAATTCACCGCCCGTCTCGTCGGAAAGCGCCTACTCTGCCAAAGAACTGAGACAGATCTTTAACCAAATGGATACGAATGAAGACGGAGGATTAGGATTGTTCGAGATCAAGAACGCATTGAAGAAACTGGACGCTGAAATGCCAGTTCATCTCGCTTACAGTGCTCTGTGTCACGCAGATCGAAACAGAGATGGAAAGCTTGGCGAGGAAGAGATCGATCAGTTCGTAAAGTACATGATGAGCAAAGGTTATGTTGTCTGA

Protein sequence

MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV*

Homology

[Close]

BLAST of CSPI04G24020 vs. ExPASy TrEMBL
Match: A0A0A0L3J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.4e-47
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60
          MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV
Sbjct: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60

Query: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of CSPI04G24020 vs. ExPASy TrEMBL
Match: A0A5D3D9N2 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003460 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.2e-39
Identity = 85/88 (96.59%), Postives = 86/88 (97.73%), Query Frame = 0


Query: 10 KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
          KSNS  VSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALC+
Sbjct: 2  KSNSRAVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCY 61

Query: 70 ADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          ADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 62 ADRNRDGKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of CSPI04G24020 vs. ExPASy TrEMBL
Match: A0A6A1UYX3 (Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G017070 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-09
Identity = 33/76 (43.42%), Postives = 47/76 (61.84%), Query Frame = 0


Query: 22  YSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCHADRNRDGKLGEE 81
           Y+ ++LR +F + DTN DG L   E++NA + L +  P   A+ AL HADRN DG++ E 
Sbjct: 31  YTGEQLRGMFKRFDTNGDGQLSRQELRNAFRSLGSAAPGWRAFRALGHADRNGDGQISEA 90

Query: 82  EIDQFVKYMMSKGYVV 98
           E+D  V Y +  GY V
Sbjct: 91  ELDNLVGYALKHGYTV 106

[Close]

BLAST of CSPI04G24020 vs. ExPASy TrEMBL
Match: W9S022 (Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_000100 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.5e-09
Identity = 36/90 (40.00%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 8   KTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSAL 67
           K+  N  P    + Y  ++L+ +  + DT+ DG L   E+K A K+L A  P   A  AL
Sbjct: 13  KSVQNHSP--KPAVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRAL 72

Query: 68  CHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
           CHAD+NRDG + EEE+   V Y   +GYV+
Sbjct: 73  CHADKNRDGFISEEELANLVHYAAQRGYVL 100

[Close]

BLAST of CSPI04G24020 vs. ExPASy TrEMBL
Match: A0A6J1AEM7 (probable calcium-binding protein CML15 OS=Herrania umbratica OX=108875 GN=LOC110417197 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.7e-09
Identity = 37/87 (42.53%), Postives = 51/87 (58.62%), Query Frame = 0


Query: 15  PVSSE-----SAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 74
           PVSS+        S  ++R IFN+ D N+DG L   E+K+A   L + MP   A+ AL H
Sbjct: 34  PVSSDIKGAAVVVSEDQVRGIFNRFDANKDGRLSKQELKDAFASLGSRMPGVRAWLALQH 93

Query: 75  ADRNRDGKLGEEEIDQFVKYMMSKGYV 97
           AD N DG + E E D+ VKY + +GY+
Sbjct: 94  ADANGDGYISEAEFDKLVKYTLKRGYI 120

[Close]

BLAST of CSPI04G24020 vs. NCBI nr
Match: KGN55187.1 (hypothetical protein Csa_012728 [Cucumis sativus])

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-46
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60
          MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV
Sbjct: 1  MSSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPV 60

Query: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 61 HLAYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of CSPI04G24020 vs. NCBI nr
Match: KAA0064378.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 170.6 bits (431), Expect = 6.6e-39
Identity = 85/88 (96.59%), Postives = 86/88 (97.73%), Query Frame = 0


Query: 10 KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
          KSNS  VSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALC+
Sbjct: 2  KSNSRAVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCY 61

Query: 70 ADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          ADRNRDGKLGEEEIDQFVKYMMSKGYVV
Sbjct: 62 ADRNRDGKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of CSPI04G24020 vs. NCBI nr
Match: KAG7024250.1 (hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 148.7 bits (374), Expect = 2.7e-32
Identity = 72/95 (75.79%), Postives = 83/95 (87.37%), Query Frame = 0


Query: 3  SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62
          S FRPK       ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HL
Sbjct: 2  SFFRPK-------LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHL 61

Query: 63 AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
          AY ALCHADRN DGKLGEEEID+FVKYM+S+GYVV
Sbjct: 62 AYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV 89

[Close]

BLAST of CSPI04G24020 vs. NCBI nr
Match: KAG7037807.1 (hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 142.9 bits (359), Expect = 1.5e-30
Identity = 67/88 (76.14%), Postives = 77/88 (87.50%), Query Frame = 0


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           KS +    SES YSAKELR+IFNQMDTNEDGGLGLFEIK ALK+LDAE PVHLA+ ALC 
Sbjct: 47  KSKAVAAQSESGYSAKELREIFNQMDTNEDGGLGLFEIKEALKQLDAEFPVHLAFKALCQ 106

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
           +DRNRDGKLG +EID+F+KY+MS+GYVV
Sbjct: 107 SDRNRDGKLGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of CSPI04G24020 vs. NCBI nr
Match: KAG6608479.1 (hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142.9 bits (359), Expect = 1.5e-30
Identity = 67/88 (76.14%), Postives = 77/88 (87.50%), Query Frame = 0


Query: 10  KSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCH 69
           KS +    SES YSAKELR+IFNQMDTNEDGGLGLFEIK ALK+LDAE PVHLA+ ALC 
Sbjct: 47  KSKAVAAQSESGYSAKELREIFNQMDTNEDGGLGLFEIKEALKQLDAEFPVHLAFKALCQ 106

Query: 70  ADRNRDGKLGEEEIDQFVKYMMSKGYVV 98
           +DRNRDGKLG +EID+F+KY+MS+GYVV
Sbjct: 107 SDRNRDGKLGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of CSPI04G24020 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 40.4 bits (93), Expect = 9.6e-04
Identity = 22/81 (27.16%), Postives = 41/81 (50.62%), Query Frame = 0


Query: 2   SSMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVH 61
           S++  P++ S+          S +ELRQ+F+  D++ DG +  FE+++    +   +   
Sbjct: 40  STLNSPRSNSDDNNNIKSHQASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHE 99

Query: 62  LAYSALCHADRNRDGKLGEEE 83
            A  A+   D + DG LG E+
Sbjct: 100 AAQEAINEVDTDADGSLGFED 120

The following BLAST results are available for this feature:

BLAST of CSPI04G24020 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI04G24020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3J7	5.4e-47	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1	[more]
A0A5D3D9N2	3.2e-39	96.59	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6A1UYX3	1.5e-09	43.42	Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G0...	[more]
W9S022	4.5e-09	40.00	Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_0001...	[more]
A0A6J1AEM7	7.7e-09	42.53	probable calcium-binding protein CML15 OS=Herrania umbratica OX=108875 GN=LOC110...	[more]

BLAST of CSPI04G24020 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN55187.1	1.1e-46	100.00	hypothetical protein Csa_012728 [Cucumis sativus]	[more]
KAA0064378.1	6.6e-39	96.59	putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 pu...	[more]
KAG7024250.1	2.7e-32	75.79	hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG7037807.1	1.5e-30	76.14	hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6608479.1	1.5e-30	76.14	hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI04G24020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	9.6e-04	27.16	calmodulin-like 41	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 62..90 e-value: 16.0 score: 7.9 coord: 26..54 e-value: 0.074 score: 22.2
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 26..54 e-value: 4.0E-5 score: 23.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 58..93 score: 8.766953
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 22..57 score: 11.528597
None	No IPR available	GENE3D	1.10.238.10		coord: 5..95 e-value: 9.8E-13 score: 50.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 71..83
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 24..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G24020.1	CSPI04G24020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding