CSPI04G23510 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G23510
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionUbiquitin-conjugating enzyme family protein
LocationChr4: 21589943 .. 21595258 (+)
RNA-Seq ExpressionCSPI04G23510
SyntenyCSPI04G23510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTGTCTCTTCCTATTCTTCTTCTCTAAATCATCCGATTGTTGTTTTTTTCTCGCTATGTCATCAGGAAACGAGAACTATCATCTGGTTCCGCTTCTGGGTTTGTGAAATCCCACCACCCGATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTATAACTATAATTTTGGTTTCTATCTCTCTATTTCTATGTGTGATTGTTTTGTTTTTGTTCAACACTTTCCGCTTGATCCATGTTTATGGTTTGATCATCGAGTGTGATGTACCTCATTTTTGTATCACTTTTTTGGGTTGAGTCAATTTCAAACTTTTTCCAACTTTTGCTACTGGCCCACTACGGATGATTGAAATTTATGAGTTGGGTATTGTTTTTTTTTTTTTAGTGTAGTGTTTGTGAAATCTTGTTTGGAAATTCGGGAATTACGATCGTGTTTTATGTCTGGAAGGTGTTTGTTGAACTGGAGTTAGCGCTGCAGTTTTCATGCCACATTGTGTTGTCTTTAAATTGATTTTCTTCCATTTAGTATCGTTGCATGATTGGATAGTTATCAATTCTGTGAACTGAGTGGAAGTGACGCTTGATTGTTGCTTTATATTGTAGTTTTCTCTTATTTGGAGCACTAACTGGTCTTGAATTATTAGAAGCCAATTATGAGAGACATCTAGCGTATTGAAAACTCTTAAAAGTCTATTTCGATTGTGCTTTGGTCAGATGAAAGTCATCAGCAAAGTGATATTTCTTGCTGTTGTTGTTAGCTCTCATATTTTTCATATTCTAATTCTTTTTCACTTACATTCACTGACACAGAAAGGGAAAAGATCCAAATTTTATAATGTGATGGTTGCATTTGAATTGGAATTCTTTCGATGAGTGAAAAATTGACCATAATATTAGAGTGACAGGGAGTGAAGTGTCAGTCAATAAGTAGCGCTGAAAAAGAAATTGTATGAGATTGAGCAATGGTATTGTTTTGCACCAGTAAATAATCTTCTATGCCAGTGCCCAATGTGTTTATACAAAATAAACTTTAGATTTGTATGGTTTTATTCTTGTCTATAACTAAATATATCTTGTGTGTTATTCTGATGCTCTTTTGACATCTTGCATTGTCTGGACATTCATCTGGTTAGCTATGCTGCTGGAAGAAGGCATGCCCTGCAATGTCCCCTTTTGTTTCATACTTTCATCTTTTCTTCTTTCGTTTTTTCTACTCATGATCTATTTCCGAAAATAAAACTTTTCTTTTTTCTACTCATGCCACTGCATTTTATACACATAATTTAGGTTGATCACCTTGGTTGTTCAATGAAGGATTGAAGCACAAAACTACCCATTCTGATATCTTTATTTGTTCTTCCCCTTCTAGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGGTTCGTTATCACAGTATTTGTTCATTGCTTGTTTGGTGTCTTCTTTCACATCTGTTTTCCACTTTTCTTTTCTTATGGAAACAGAGGAAATGTGATTTACTTTAAGATGTTACAAAGAAATGAAAGGATATGGCATCCTCATGTAAAGTACGAGATACAATATCACCTACTGTATATACTAGCAAAAAACCAATGTCTAAAGATTAATTATTTCTGAAACAATTACCCTGCAACTTCAGCCCTTTTTAAATACCGTCTACTTCTTCTAACCTAATTATTCAATAAATTATATAAACCACACCTTGCCACTTGCCAGAATGTTACTTTTTTTTCCTCTTTTTTCTCCCCAAATAACATTAAATACTTGCTTTCGAAGATCTATTTACAAAATGTGTGATCCAGTGTCTTGTTAAGGTCAAGCTTATTGACATGTTACGTTTTTGAAATCTGTATTCACATTTTTTTCTTTTTAAAAGAACCACTTGCCTTGTTCTTTTTAGAAAAAACTATAGGTGAAGTAAATTAGTATAAAACAACAATAAACTACAATCGCCTACACATGTTTTTATTATAGTTTGATCCAAAATATGAGAATTTTATTTTTCCCTAATGGCTCTAAAACAATCTGAAGCCCATATTTGGTTTTGATGATCAAACCCTATGAACAGAAGAGTTCCAACAAATTTTTTATAACTTTTGACCTAATTCACTTTTTTTAAAAACATACTCTAGGTATAAGCACCGAAACCATGCTTTAGTTGAAGTTTAACGACACTTTCTTTGATAATTAGGAGTCGTTGAAGATGTAAGTTTTGTAAATTGAAACAGACATGGTATGCTTGCGATACCTGTTCTAATTATTCATAATTATAGCTAAAGTTTTTACTTTGTTCAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCGGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAAAAAACCAGCTGCATCTCGCAGGGACAAACATGAGGTTAGTCACTTAAATTCTTTACTTGGTATATATGCAGCTAAATCCAGATGGTTTTTGGATCCTTTTAAGCATAAAAAGAAGCTACATGGTTCAAATAACATTTCTATTAACTCGTCGGATGCAATGAAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTTATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGTATAAGTCTTGAAAAATTATTCTTTTGTTTATGAATACTTTTATTTATTTCAGGAGGTTTTAGACATTCTCTTGTTGCCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGGTAGGTTTCCCTTTTCTTTTCCTCCATTTTGAACATATTGTAGATTCATTTTAACATAAGCTCTGAAAGATTTAGCTGTAGGAAATGTCAAGCTTTTGAATAAACGGTAATTGACTTCACTTTTATCCTTTTGAATTCTATACCACAGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTTCTTTGACATATTTTTCCCTCCTCGTTATCCAGACCTACCACCAGTATGTGTATATGTTCATAATTTTTGGTGTTTATTCTATAGTTATTACTATACTGTGCTTGGATTTTCTGACAGACAATGTTCTCTTAAATGCAGCAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGGTATGGTTCTAGACATCTTTTCCTGTTTGGGGCCTTTTAGTAGGTTGAATACAATATCAAGAGATGGTATTATCTTAGAACTGAAGGATAAGATGTACAACCTATTAAAACAGATCTTGTTTAGTGAGGTCCAGGTGGGATTATATTAGTAACTAAGATTGTAAATTTTAGCATTCGAGTAGGATGCTTAATAAGAGAATAGCCTTGTCATTACTTAGGAGGTAGATTGGGACGTGCAAATATCAAAGCGGAGTTTGTTAAATGTATTTAGTTGTAATGTTTTATCAAGTGTATGCTTATTTAAATGATGTTCATGACCCTTGTTGTTGAATAAATTAAGACTAGAATAACTTCTGCTGCAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAGAATAGAAAGGGTTGGAAGGCGATGGTGTCGGGAAGTTGGTTTTTGGTATAGATTTTGAACATGTTGACGTCTAACTGAAACAAACTTGAGATTTGTTAACTGTCCAAGGTAGTTTAGAAGGTTAGCGTTTAGAGCAATCATAGTAGAGCTTGCTTGCTTCCCTATGTCTATAATTTTTCTCATAATGAACACATATGATCTCTAATTGTGTACATAGTCTCTATATGCACCAGAGGATAATGTCTTTCCATTTCAACACGGACAGTGGTATACTATGTAACTTATTTTCTTCCTCTCAAGGTAATAATGACGCTTTTTGGTTTTCA

mRNA sequence

TCTTTGTCTCTTCCTATTCTTCTTCTCTAAATCATCCGATTGTTGTTTTTTTCTCGCTATGTCATCAGGAAACGAGAACTATCATCTGGTTCCGCTTCTGGGTTTGTGAAATCCCACCACCCGATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCGGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAAAAAACCAGCTGCATCTCGCAGGGACAAACATGAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTTATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTTCTTTGACATATTTTTCCCTCCTCGTTATCCAGACCTACCACCACAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAGAATAGAAAGGGTTGGAAGGCGATGGTGTCGGGAAGTTGGTTTTTGGTATAGATTTTGAACATGTTGACGTCTAACTGAAACAAACTTGAGATTTGTTAACTGTCCAAGGTAGTTTAGAAGGTTAGCGTTTAGAGCAATCATAGTAGAGCTTGCTTGCTTCCCTATGTCTATAATTTTTCTCATAATGAACACATATGATCTCTAATTGTGTACATAGTCTCTATATGCACCAGAGGATAATGTCTTTCCATTTCAACACGGACAGTGGTATACTATGTAACTTATTTTCTTCCTCTCAAGGTAATAATGACGCTTTTTGGTTTTCA

Coding sequence (CDS)

ATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCGGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAAAAAACCAGCTGCATCTCGCAGGGACAAACATGAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTTATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTTCTTTGACATATTTTTCCCTCCTCGTTATCCAGACCTACCACCACAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAG

Protein sequence

MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGSHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQVQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK*
Homology
BLAST of CSPI04G23510 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 2.2e-101
Identity = 172/278 (61.87%), Postives = 217/278 (78.06%), Query Frame = 0

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    +FK+FDT++D SDHHYASKG S  Q SK W KK+Q DWKIL+NDLPE I V
Sbjct: 237 KEEFL---RDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISV 296

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP V+YHS GLR+NPNLYNC
Sbjct: 297 RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNC 356

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW G+  E W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+
Sbjct: 357 GKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGES 416

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM+++IRRPP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 417 KSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 476

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 477 KGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of CSPI04G23510 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 3.2e-100
Identity = 168/278 (60.43%), Postives = 216/278 (77.70%), Query Frame = 0

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    NFK+FD+++D SDHHY SKG +  Q SK W KK+QD+WKIL  +LPETIFV
Sbjct: 20  KEEFL---RNFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFV 79

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP VYYHS GLR+NPNLYNC
Sbjct: 80  RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNC 139

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW GN  ++W+P  STMLQVLVSIQ L+LN +PYFNEPGY    G+  GE+
Sbjct: 140 GKVCLSLLGTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGES 199

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM++++R+PP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 200 KSKVYSENVFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 259

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 260 KGGVQDLEQARQSGSKKFKTDVASFMQTVVDEFVKLGV 294

BLAST of CSPI04G23510 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.0e-99
Identity = 241/610 (39.51%), Postives = 322/610 (52.79%), Query Frame = 0

Query: 18  DVIDVDK-DEASDAMFIHQKFDANDKGKGI----YHHGDHQLKKTLGSHSFNPDVEILES 77
           +VIDV++ D  +  +      D  +KGK I      +GD+  +      S   D    ES
Sbjct: 29  EVIDVEEYDLQNGGVPNGNNVDYKNKGKAIDFDSMSYGDYGEEDEYAVGSPGDDYGYPES 88

Query: 78  TNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEF---MDVDAYSALQAHFDHMDIP 137
           +          S++L+D   P S    +D+++    +F    + D YS  Q  FD  DIP
Sbjct: 89  S--------PLSNSLLD---PESLIYEDDENYSEQYDFEMEAEPDNYSMYQDLFDGKDIP 148

Query: 138 PDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQVQVKEVSNGL 197
             +E  + W       N  + E++ +S S+ A       + N+  K  T+      ++G+
Sbjct: 149 TGVEVSMDWFP-----NSADKESASSSKSSHA------NNGNNSSKKATK------ASGI 208

Query: 198 HLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVKKQVTSHG 257
           H     ++   +       P +  P+K         + L    +     Y+V        
Sbjct: 209 HSQFSSDMETPVAQ-----PWNALPHKAEGVIPNSAYALPQNSKAFQPPYAVHYSAL--- 268

Query: 258 MPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGE 317
                      F  YL      PQ P+    +   P      A+G  G   P  P     
Sbjct: 269 --------KTAFSNYL-----QPQTPDTVLGEAPAP------AAGSSGLLPPNTPGFKSN 328

Query: 318 VVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKG 377
                      +  P + P+DS  +     + + +LG +  FK+FD ++D SDHHYASKG
Sbjct: 329 AARF------KEEPPILPPDDSRVK----RNMEDYLGLYLFFKRFDIVEDFSDHHYASKG 388

Query: 378 SSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFF 437
           ++  Q SK W K+IQD+W+IL+ DLPE IFVR YESRMDL+RAVIIGAQGTPYHDGLFFF
Sbjct: 389 TTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFF 448

Query: 438 DIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQV 497
           DIFFP  YP  PP V+YHS GLR+NPNLYNCGKVCLSLL TW GN  E W+P  STMLQV
Sbjct: 449 DIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQV 508

Query: 498 LVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFE 557
           LVSIQGLILN KPYFNEPGY   +GS  GE+ S  Y+E T+ILS+KTM++ +RRPPK+FE
Sbjct: 509 LVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFE 568

Query: 558 DFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSV-THLLSQ 617
           DF  GHF+  AHD+L AC AY NGA  G LV+G     E S    S  F+  V T + + 
Sbjct: 569 DFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENSAGMSSLKFRTDVATFVETV 573

Query: 618 LVAAFKGIGV 619
           L+  F  +GV
Sbjct: 629 LLKEFILLGV 573

BLAST of CSPI04G23510 vs. ExPASy Swiss-Prot
Match: F4HPP7 (Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=UBC39 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.3e-82
Identity = 154/286 (53.85%), Postives = 198/286 (69.23%), Query Frame = 0

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGS---------SLNQASKKWTKKIQDDWKILQ 400
           K++FL    +FK+FDT++D SDHHYA++GS               K W K IQ +WKIL 
Sbjct: 14  KEEFL---RDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILD 73

Query: 401 NDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGL 460
            +LPETIFVR  ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP+V+YHS GL
Sbjct: 74  KNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGL 133

Query: 461 RLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAY 520
           R+NPNLY CGKVCLSL++TW G   E W+P  STMLQ+LVSIQ LILN KPY+NEPGY  
Sbjct: 134 RINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEK 193

Query: 521 QSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYI 580
             G+  GE+ S  Y+E  ++ S+KTM         HFE+FVR HF+ R+HDI+ AC AY 
Sbjct: 194 SMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYK 253

Query: 581 NGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 618
           +GA VGS+ +GGV+  + + +  S  F+ +VT  +  +V  F  +G
Sbjct: 254 DGAPVGSIDKGGVK--KQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of CSPI04G23510 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.8e-79
Identity = 145/277 (52.35%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 347 NFENFKQFDTIDDHSDHHYAS--KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYE 406
           N   F+QFD ++D SDHH+ S  KG + +Q +K W KK+Q +W  L+ +LP TI+VRV E
Sbjct: 629 NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCE 688

Query: 407 SRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVC 466
            RMDL+RA ++GA GTPYHDGLFFFDI  PP+YP  PP V+YHS G+RLNPNLY  G+VC
Sbjct: 689 ERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVC 748

Query: 467 LSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHM 526
           LSLLNTW G+G E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S  
Sbjct: 749 LSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVS 808

Query: 527 YNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGV 586
           YNE  ++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAY+ G  VGS      
Sbjct: 809 YNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA---- 868

Query: 587 QDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDC 622
            +L+ +  + S  FK  ++ L  +L+ AF  IGV DC
Sbjct: 869 -NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DC 899

BLAST of CSPI04G23510 vs. ExPASy TrEMBL
Match: A0A0A0KZM7 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637700 PE=4 SV=1)

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240

Query: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300
           VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN
Sbjct: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300

Query: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360
           PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH
Sbjct: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360

Query: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420
           SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT
Sbjct: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420

Query: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480
           PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV
Sbjct: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480

Query: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540
           PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN
Sbjct: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540

Query: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600
           IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ
Sbjct: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600

Query: 601 SVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           SVTHLLSQLVAAFKGIGV DCDKYLQMLSPTVPNTRKRPSRPVKLK
Sbjct: 601 SVTHLLSQLVAAFKGIGVSDCDKYLQMLSPTVPNTRKRPSRPVKLK 646

BLAST of CSPI04G23510 vs. ExPASy TrEMBL
Match: A0A5D3D9M1 (Putative ubiquitin-conjugating enzyme E2 25 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003950 PE=4 SV=1)

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 627/647 (96.91%), Postives = 635/647 (98.15%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 92

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 93  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 152

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 153 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 212

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASY 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHELHNGGETSDASY
Sbjct: 213 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHELHNGGETSDASY 272

Query: 241 SVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 300
           SVKKQVTSH MPHPNFPESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSF
Sbjct: 273 SVKKQVTSHSMPHPNFPESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 332

Query: 301 NPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDD 360
           NPFVPPTLGEVVDVPWVQGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DD
Sbjct: 333 NPFVPPTLGEVVDVPWVQGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDD 392

Query: 361 HSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 420
           HSDHHYA+KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG
Sbjct: 393 HSDHHYATKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 452

Query: 421 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 480
           TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW
Sbjct: 453 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 512

Query: 481 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 540
           VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF
Sbjct: 513 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 572

Query: 541 NIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 600
           NIRRPPKHFEDFVRGHFY+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK
Sbjct: 573 NIRRPPKHFEDFVRGHFYKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 632

Query: 601 QSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           QSVTHLLSQLV AFKGIGVGDCDKYLQMLSPT+PN RKRP RPVK+K
Sbjct: 633 QSVTHLLSQLVGAFKGIGVGDCDKYLQMLSPTIPNARKRPGRPVKIK 679

BLAST of CSPI04G23510 vs. ExPASy TrEMBL
Match: A0A1S3BUP3 (probable ubiquitin-conjugating enzyme E2 25 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493488 PE=4 SV=1)

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 627/647 (96.91%), Postives = 635/647 (98.15%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 92

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 93  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 152

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 153 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 212

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASY 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHELHNGGETSDASY
Sbjct: 213 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHELHNGGETSDASY 272

Query: 241 SVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 300
           SVKKQVTSH MPHPNFPESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSF
Sbjct: 273 SVKKQVTSHSMPHPNFPESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 332

Query: 301 NPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDD 360
           NPFVPPTLGEVVDVPWVQGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DD
Sbjct: 333 NPFVPPTLGEVVDVPWVQGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDD 392

Query: 361 HSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 420
           HSDHHYA+KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG
Sbjct: 393 HSDHHYATKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 452

Query: 421 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 480
           TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW
Sbjct: 453 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 512

Query: 481 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 540
           VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF
Sbjct: 513 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 572

Query: 541 NIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 600
           NIRRPPKHFEDFVRGHFY+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK
Sbjct: 573 NIRRPPKHFEDFVRGHFYKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 632

Query: 601 QSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           QSVTHLLSQLV AFKGIGVGDCDKYLQMLSPT+PN RKRP RPVK+K
Sbjct: 633 QSVTHLLSQLVGAFKGIGVGDCDKYLQMLSPTIPNARKRPGRPVKIK 679

BLAST of CSPI04G23510 vs. ExPASy TrEMBL
Match: A0A5A7VF42 (Putative ubiquitin-conjugating enzyme E2 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00300 PE=4 SV=1)

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 627/690 (90.87%), Postives = 635/690 (92.03%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 23  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 82

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 83  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 142

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 143 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 202

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE------------ 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHE            
Sbjct: 203 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHEVSHLNSLLGIDA 262

Query: 241 -------------------------------LHNGGETSDASYSVKKQVTSHGMPHPNFP 300
                                          LHNGGETSDASYSVKKQVTSH MPHPNFP
Sbjct: 263 AKSRWFLDPFKRKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHSMPHPNFP 322

Query: 301 ESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360
           ESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV
Sbjct: 323 ESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 382

Query: 361 QGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQAS 420
           QGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DDHSDHHYA+KGSSLNQAS
Sbjct: 383 QGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDDHSDHHYATKGSSLNQAS 442

Query: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480
           KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR
Sbjct: 443 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 502

Query: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540
           YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL
Sbjct: 503 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 562

Query: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600
           ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF
Sbjct: 563 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 622

Query: 601 YQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGI 647
           Y+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV AFKGI
Sbjct: 623 YKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVGAFKGI 682

BLAST of CSPI04G23510 vs. ExPASy TrEMBL
Match: A0A1S3BTU8 (probable ubiquitin-conjugating enzyme E2 25 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493488 PE=4 SV=1)

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 627/690 (90.87%), Postives = 635/690 (92.03%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 60

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE------------ 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHE            
Sbjct: 181 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHEVSHLNSLLGIDA 240

Query: 241 -------------------------------LHNGGETSDASYSVKKQVTSHGMPHPNFP 300
                                          LHNGGETSDASYSVKKQVTSH MPHPNFP
Sbjct: 241 AKSRWFLDPFKRKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHSMPHPNFP 300

Query: 301 ESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360
           ESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV
Sbjct: 301 ESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360

Query: 361 QGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQAS 420
           QGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DDHSDHHYA+KGSSLNQAS
Sbjct: 361 QGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDDHSDHHYATKGSSLNQAS 420

Query: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480
           KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR
Sbjct: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480

Query: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540
           YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL
Sbjct: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540

Query: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600
           ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF
Sbjct: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600

Query: 601 YQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGI 647
           Y+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV AFKGI
Sbjct: 601 YKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVGAFKGI 660

BLAST of CSPI04G23510 vs. NCBI nr
Match: XP_004141363.1 (probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis sativus])

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240

Query: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300
           VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN
Sbjct: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300

Query: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360
           PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH
Sbjct: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360

Query: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420
           SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT
Sbjct: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420

Query: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480
           PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV
Sbjct: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480

Query: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540
           PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN
Sbjct: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540

Query: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600
           IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ
Sbjct: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600

Query: 601 SVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           SVTHLLSQLVAAFKGIGV DCDKYLQMLSPTVPNTRKRPSRPVKLK
Sbjct: 601 SVTHLLSQLVAAFKGIGVSDCDKYLQMLSPTVPNTRKRPSRPVKLK 646

BLAST of CSPI04G23510 vs. NCBI nr
Match: KAE8649845.1 (hypothetical protein Csa_012313 [Cucumis sativus])

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 645/689 (93.61%), Postives = 645/689 (93.61%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 92

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 93  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 152

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 153 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 212

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE------------- 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE             
Sbjct: 213 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHEVSHLNSLLGIYAA 272

Query: 241 ------------------------------LHNGGETSDASYSVKKQVTSHGMPHPNFPE 300
                                         LHNGGETSDASYSVKKQVTSHGMPHPNFPE
Sbjct: 273 KSRWFLDPFKHKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHGMPHPNFPE 332

Query: 301 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 360
           SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ
Sbjct: 333 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 392

Query: 361 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 420
           GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK
Sbjct: 393 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 452

Query: 421 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 480
           KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY
Sbjct: 453 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 512

Query: 481 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 540
           PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI
Sbjct: 513 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 572

Query: 541 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 600
           LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY
Sbjct: 573 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 632

Query: 601 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 647
           QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG
Sbjct: 633 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 692

BLAST of CSPI04G23510 vs. NCBI nr
Match: XP_011654074.1 (probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis sativus])

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 645/689 (93.61%), Postives = 645/689 (93.61%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE------------- 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE             
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHEVSHLNSLLGIYAA 240

Query: 241 ------------------------------LHNGGETSDASYSVKKQVTSHGMPHPNFPE 300
                                         LHNGGETSDASYSVKKQVTSHGMPHPNFPE
Sbjct: 241 KSRWFLDPFKHKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHGMPHPNFPE 300

Query: 301 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 360
           SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ
Sbjct: 301 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 360

Query: 361 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 420
           GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK
Sbjct: 361 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 420

Query: 421 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 480
           KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY
Sbjct: 421 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 480

Query: 481 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 540
           PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI
Sbjct: 481 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 540

Query: 541 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 600
           LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY
Sbjct: 541 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 600

Query: 601 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 647
           QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG
Sbjct: 601 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 660

BLAST of CSPI04G23510 vs. NCBI nr
Match: XP_008452464.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X3 [Cucumis melo] >TYK20255.1 putative ubiquitin-conjugating enzyme E2 25 isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 627/647 (96.91%), Postives = 635/647 (98.15%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 92

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 93  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 152

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 153 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 212

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASY 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHELHNGGETSDASY
Sbjct: 213 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHELHNGGETSDASY 272

Query: 241 SVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 300
           SVKKQVTSH MPHPNFPESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSF
Sbjct: 273 SVKKQVTSHSMPHPNFPESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 332

Query: 301 NPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDD 360
           NPFVPPTLGEVVDVPWVQGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DD
Sbjct: 333 NPFVPPTLGEVVDVPWVQGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDD 392

Query: 361 HSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 420
           HSDHHYA+KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG
Sbjct: 393 HSDHHYATKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 452

Query: 421 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 480
           TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW
Sbjct: 453 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 512

Query: 481 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 540
           VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF
Sbjct: 513 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 572

Query: 541 NIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 600
           NIRRPPKHFEDFVRGHFY+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK
Sbjct: 573 NIRRPPKHFEDFVRGHFYKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 632

Query: 601 QSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           QSVTHLLSQLV AFKGIGVGDCDKYLQMLSPT+PN RKRP RPVK+K
Sbjct: 633 QSVTHLLSQLVGAFKGIGVGDCDKYLQMLSPTIPNARKRPGRPVKIK 679

BLAST of CSPI04G23510 vs. NCBI nr
Match: XP_008452463.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis melo])

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 627/690 (90.87%), Postives = 635/690 (92.03%), Query Frame = 0

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 60

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHE------------ 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHE            
Sbjct: 181 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHEVSHLNSLLGIDA 240

Query: 241 -------------------------------LHNGGETSDASYSVKKQVTSHGMPHPNFP 300
                                          LHNGGETSDASYSVKKQVTSH MPHPNFP
Sbjct: 241 AKSRWFLDPFKRKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHSMPHPNFP 300

Query: 301 ESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360
           ESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV
Sbjct: 301 ESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360

Query: 361 QGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQAS 420
           QGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DDHSDHHYA+KGSSLNQAS
Sbjct: 361 QGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDDHSDHHYATKGSSLNQAS 420

Query: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480
           KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR
Sbjct: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480

Query: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540
           YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL
Sbjct: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540

Query: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600
           ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF
Sbjct: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600

Query: 601 YQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGI 647
           Y+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV AFKGI
Sbjct: 601 YKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVGAFKGI 660

BLAST of CSPI04G23510 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 371.3 bits (952), Expect = 1.6e-102
Identity = 172/278 (61.87%), Postives = 217/278 (78.06%), Query Frame = 0

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    +FK+FDT++D SDHHYASKG S  Q SK W KK+Q DWKIL+NDLPE I V
Sbjct: 237 KEEFL---RDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISV 296

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP V+YHS GLR+NPNLYNC
Sbjct: 297 RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNC 356

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW G+  E W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+
Sbjct: 357 GKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGES 416

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM+++IRRPP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 417 KSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 476

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 477 KGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of CSPI04G23510 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 363.2 bits (931), Expect = 4.3e-100
Identity = 241/610 (39.51%), Postives = 322/610 (52.79%), Query Frame = 0

Query: 18  DVIDVDK-DEASDAMFIHQKFDANDKGKGI----YHHGDHQLKKTLGSHSFNPDVEILES 77
           +VIDV++ D  +  +      D  +KGK I      +GD+  +      S   D    ES
Sbjct: 29  EVIDVEEYDLQNGGVPNGNNVDYKNKGKAIDFDSMSYGDYGEEDEYAVGSPGDDYGYPES 88

Query: 78  TNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEF---MDVDAYSALQAHFDHMDIP 137
           +          S++L+D   P S    +D+++    +F    + D YS  Q  FD  DIP
Sbjct: 89  S--------PLSNSLLD---PESLIYEDDENYSEQYDFEMEAEPDNYSMYQDLFDGKDIP 148

Query: 138 PDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQVQVKEVSNGL 197
             +E  + W       N  + E++ +S S+ A       + N+  K  T+      ++G+
Sbjct: 149 TGVEVSMDWFP-----NSADKESASSSKSSHA------NNGNNSSKKATK------ASGI 208

Query: 198 HLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVKKQVTSHG 257
           H     ++   +       P +  P+K         + L    +     Y+V        
Sbjct: 209 HSQFSSDMETPVAQ-----PWNALPHKAEGVIPNSAYALPQNSKAFQPPYAVHYSAL--- 268

Query: 258 MPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGE 317
                      F  YL      PQ P+    +   P      A+G  G   P  P     
Sbjct: 269 --------KTAFSNYL-----QPQTPDTVLGEAPAP------AAGSSGLLPPNTPGFKSN 328

Query: 318 VVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKG 377
                      +  P + P+DS  +     + + +LG +  FK+FD ++D SDHHYASKG
Sbjct: 329 AARF------KEEPPILPPDDSRVK----RNMEDYLGLYLFFKRFDIVEDFSDHHYASKG 388

Query: 378 SSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFF 437
           ++  Q SK W K+IQD+W+IL+ DLPE IFVR YESRMDL+RAVIIGAQGTPYHDGLFFF
Sbjct: 389 TTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFF 448

Query: 438 DIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQV 497
           DIFFP  YP  PP V+YHS GLR+NPNLYNCGKVCLSLL TW GN  E W+P  STMLQV
Sbjct: 449 DIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQV 508

Query: 498 LVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFE 557
           LVSIQGLILN KPYFNEPGY   +GS  GE+ S  Y+E T+ILS+KTM++ +RRPPK+FE
Sbjct: 509 LVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFE 568

Query: 558 DFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSV-THLLSQ 617
           DF  GHF+  AHD+L AC AY NGA  G LV+G     E S    S  F+  V T + + 
Sbjct: 569 DFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENSAGMSSLKFRTDVATFVETV 573

Query: 618 LVAAFKGIGV 619
           L+  F  +GV
Sbjct: 629 LLKEFILLGV 573

BLAST of CSPI04G23510 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 308.1 bits (788), Expect = 1.6e-83
Identity = 154/286 (53.85%), Postives = 198/286 (69.23%), Query Frame = 0

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGS---------SLNQASKKWTKKIQDDWKILQ 400
           K++FL    +FK+FDT++D SDHHYA++GS               K W K IQ +WKIL 
Sbjct: 14  KEEFL---RDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILD 73

Query: 401 NDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGL 460
            +LPETIFVR  ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP+V+YHS GL
Sbjct: 74  KNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGL 133

Query: 461 RLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAY 520
           R+NPNLY CGKVCLSL++TW G   E W+P  STMLQ+LVSIQ LILN KPY+NEPGY  
Sbjct: 134 RINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEK 193

Query: 521 QSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYI 580
             G+  GE+ S  Y+E  ++ S+KTM         HFE+FVR HF+ R+HDI+ AC AY 
Sbjct: 194 SMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYK 253

Query: 581 NGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 618
           +GA VGS+ +GGV+  + + +  S  F+ +VT  +  +V  F  +G
Sbjct: 254 DGAPVGSIDKGGVK--KQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of CSPI04G23510 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-80
Identity = 145/277 (52.35%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 347 NFENFKQFDTIDDHSDHHYAS--KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYE 406
           N   F+QFD ++D SDHH+ S  KG + +Q +K W KK+Q +W  L+ +LP TI+VRV E
Sbjct: 629 NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCE 688

Query: 407 SRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVC 466
            RMDL+RA ++GA GTPYHDGLFFFDI  PP+YP  PP V+YHS G+RLNPNLY  G+VC
Sbjct: 689 ERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVC 748

Query: 467 LSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHM 526
           LSLLNTW G+G E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S  
Sbjct: 749 LSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVS 808

Query: 527 YNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGV 586
           YNE  ++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAY+ G  VGS      
Sbjct: 809 YNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA---- 868

Query: 587 QDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDC 622
            +L+ +  + S  FK  ++ L  +L+ AF  IGV DC
Sbjct: 869 -NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DC 899

BLAST of CSPI04G23510 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 297.7 bits (761), Expect = 2.2e-80
Identity = 155/324 (47.84%), Postives = 206/324 (63.58%), Query Frame = 0

Query: 305  PTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFL---GNFENFKQFDTIDDHS 364
            P+   V D    +G+  N  NI   ++ +      D+DK +   G+  +F++FD   D  
Sbjct: 778  PSKVNVTDNCESKGTQANAKNILSGETSTFL---EDEDKPVPSEGDSCSFRRFDISQDPL 837

Query: 365  DHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTP 424
            DHH+        +  ++W KK+  DWKILQN+LP+ IFVR YE RMDL+RAVI+GA GTP
Sbjct: 838  DHHFLGVDGQKTK-ERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTP 897

Query: 425  YHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVP 484
            Y DGLFFFD   P  YP +PP  YYHS G RLNPNLY  GKVCLSLLNTW G GNE W P
Sbjct: 898  YQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDP 957

Query: 485  GMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNI 544
              S++LQVLVS+QGL+LN+KPYFNE GY  Q G+  GE  S  YNE T++L+ KTM++ +
Sbjct: 958  KSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLM 1017

Query: 545  RRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV-EGGVQDLELSDKSCSDSFKQ 604
            R+PPK FE+ ++ HF +R + IL AC AY+ G  +GSL  +  V D   S  S S  FK 
Sbjct: 1018 RKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGSLTKDASVIDERSSANSTSVGFKL 1077

Query: 605  SVTHLLSQLVAAFKGIGVGDCDKY 625
             +  +  +L +A   +G  DC+++
Sbjct: 1078 MLAKIAPKLFSALSEVG-ADCNEF 1096

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GY872.2e-10161.87Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q9C9183.2e-10060.43Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q9LUQ56.0e-9939.51Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
F4HPP72.3e-8253.85Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q8VY101.8e-7952.35Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Match NameE-valueIdentityDescription
A0A0A0KZM70.0e+0099.85UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637700 PE... [more]
A0A5D3D9M10.0e+0096.91Putative ubiquitin-conjugating enzyme E2 25 isoform X3 OS=Cucumis melo var. maku... [more]
A0A1S3BUP30.0e+0096.91probable ubiquitin-conjugating enzyme E2 25 isoform X3 OS=Cucumis melo OX=3656 G... [more]
A0A5A7VF420.0e+0090.87Putative ubiquitin-conjugating enzyme E2 25 isoform X1 OS=Cucumis melo var. maku... [more]
A0A1S3BTU80.0e+0090.87probable ubiquitin-conjugating enzyme E2 25 isoform X2 OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
XP_004141363.10.0e+0099.85probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis sativus][more]
KAE8649845.10.0e+0093.61hypothetical protein Csa_012313 [Cucumis sativus][more]
XP_011654074.10.0e+0093.61probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis sativus][more]
XP_008452464.10.0e+0096.91PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X3 [Cucumis melo]... [more]
XP_008452463.10.0e+0090.87PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G53025.11.6e-10261.87Ubiquitin-conjugating enzyme family protein [more]
AT3G15355.14.3e-10039.51ubiquitin-conjugating enzyme 25 [more]
AT1G53023.11.6e-8353.85Ubiquitin-conjugating enzyme family protein [more]
AT2G33770.11.3e-8052.35phosphate 2 [more]
AT2G16920.12.2e-8047.84ubiquitin-conjugating enzyme 23 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 424..579
e-value: 4.7E-22
score: 89.3
NoneNo IPR availablePANTHERPTHR46116:SF12UBIQUITIN-CONJUGATING ENZYME E2 39-RELATEDcoord: 6..670
NoneNo IPR availablePANTHERPTHR46116(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYMEcoord: 6..670
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 387..658
e-value: 3.4E-89
score: 300.9
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 406..589
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 426..570
e-value: 6.9E-24
score: 84.1
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 421..581
score: 35.898251
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 423..550
e-value: 6.82526E-33
score: 121.54

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G23510.1CSPI04G23510.1mRNA
CSPI04G23510.2CSPI04G23510.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061631 ubiquitin conjugating enzyme activity