CSPI04G23390 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G23390
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionskin secretory protein xP2-like
LocationChr4: 21499620 .. 21501079 (+)
RNA-Seq ExpressionCSPI04G23390
SyntenyCSPI04G23390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTCTCTAAAACTCTCTCACTTTCTCTCTACATTTTCTTCCCATGTTTTCTCTCTCTCTCTCAAGCCTATACTTTCTACGTCGGTGGAAAAGATGGTTGGGTTCTCAATCCCTCTGAGAGCTACGACAATTGGGCTAATCGAAACAGGTTTAGAGTCAATGACGTTCTAGGTAAGTTCTATTTCTTCTCATCAATCAAATCGAAACAAGTATTTTGCTATGTTTTTAGCTGAGTTTAGTGGTGGCTTCTTTTTCTTTTTCAGTGTTCAATTATGCAAGAGGGTCGGATTCTGTGGCGGTGGTTGGTAAAGAGGATTATGATAAATGTGATTTGAATAATCCAATAGTGAAATTGGAAGATGGGAATTCGAAGTTCAAATTTGATCGTTCGGGAGCTTTTTATTTTGCTAGCGGGAAACAGGGGATGTGTGAAAATGGCCAGAAATTGGCGGTGGTTGTTATCTCTCAGCACTCTTTTTCTCTGTCCTCCAAACTGGCCTCCACGCCGCCAGAGATTTCACCTACTTCGCCACTGTCGTTGTCAGAGACACTCGGTTCGCCTATGCCCTCGTCAGAGATGCTCGGTTCCCCAATGCCCTCGTCAGAGACACTCGGCTCGCCAATGCCCTCGTCAGAGACACTCGGTTCGCCAATGCCCTCGTCAGAGACACTCAGTTCGTCATTACCGTCATCAGAGACACTCAGTTCTCCATTGCCATTGTCAGAGACGCTCGATCAGGACCCGACCCCATCCGATGCGACGGGACAGGCGACGAATCCTACCCCGTCCGATCAGACAGGAGAAATGACGGATTCGACCCCGTCCGATCCAACGGGGGAGTCGACGGATCCGACCCCCTCCGATCAGACAGGGGAGCCGACGACGTCTCTATCATTCGAGGCGTCCGGTTCAGAGTCGCCGATGTCTGACACACAGGGTTCAGAGGCACAATCGTCCGAGACAACTGATTCTTATTATGGAGAAAGCTCTTCGTCAGGCTCATACAGTTCTTCGTCTCCTTATGATATGTCGTCAGGGGCAGAACCCTCTACTTTGACACCAATCTCTCCATCGGCATCACCTTCTTCACCAGCGGAGGAGGGAGGAGAGCCACCAGCGGAGGAGGGGGGAGAGCCACCAGCGGAAGAGGGAGGAGAGCCACCAGCAGAGGAGGGATCAGAAGAGCCACCAGCGGACGAGGGAGGAGAGGACTCGTTGGCCATAGCTTTATCTCCGTCAATAAATTTGTTGGGTTTCATTACAGGTTTATTAAGCATGGTCATCAATGGCAGAATGATTATGAACTTCATTTAAACCACAAGAAGAAGACGGAAGAAGATAAGTTTCATAATTATTATGATTGTTGTTGCTGCTACTACTACTTAGGGTTTGTGAAAAGATTTCTCCAATAATATGGCTTTTTTATTTTTAATTTGTATTTGTATATTTCATCGC

mRNA sequence

ATGGCCTTCTCTAAAACTCTCTCACTTTCTCTCTACATTTTCTTCCCATGTTTTCTCTCTCTCTCTCAAGCCTATACTTTCTACGTCGGTGGAAAAGATGGTTGGGTTCTCAATCCCTCTGAGAGCTACGACAATTGGGCTAATCGAAACAGGTTTAGAGTCAATGACGTTCTAGTGTTCAATTATGCAAGAGGGTCGGATTCTGTGGCGGTGGTTGGTAAAGAGGATTATGATAAATGTGATTTGAATAATCCAATAGTGAAATTGGAAGATGGGAATTCGAAGTTCAAATTTGATCGTTCGGGAGCTTTTTATTTTGCTAGCGGGAAACAGGGGATGTGTGAAAATGGCCAGAAATTGGCGGTGGTTGTTATCTCTCAGCACTCTTTTTCTCTGTCCTCCAAACTGGCCTCCACGCCGCCAGAGATTTCACCTACTTCGCCACTGTCGTTGTCAGAGACACTCGGTTCGCCTATGCCCTCGTCAGAGATGCTCGGTTCCCCAATGCCCTCGTCAGAGACACTCGGCTCGCCAATGCCCTCGTCAGAGACACTCGGTTCGCCAATGCCCTCGTCAGAGACACTCAGTTCGTCATTACCGTCATCAGAGACACTCAGTTCTCCATTGCCATTGTCAGAGACGCTCGATCAGGACCCGACCCCATCCGATGCGACGGGACAGGCGACGAATCCTACCCCGTCCGATCAGACAGGAGAAATGACGGATTCGACCCCGTCCGATCCAACGGGGGAGTCGACGGATCCGACCCCCTCCGATCAGACAGGGGAGCCGACGACGTCTCTATCATTCGAGGCGTCCGGTTCAGAGTCGCCGATGTCTGACACACAGGGTTCAGAGGCACAATCGTCCGAGACAACTGATTCTTATTATGGAGAAAGCTCTTCGTCAGGCTCATACAGTTCTTCGTCTCCTTATGATATGTCGTCAGGGGCAGAACCCTCTACTTTGACACCAATCTCTCCATCGGCATCACCTTCTTCACCAGCGGAGGAGGGAGGAGAGCCACCAGCGGAGGAGGGGGGAGAGCCACCAGCGGAAGAGGGAGGAGAGCCACCAGCAGAGGAGGGATCAGAAGAGCCACCAGCGGACGAGGGAGGAGAGGACTCGTTGGCCATAGCTTTATCTCCGTCAATAAATTTGTTGGGTTTCATTACAGGTTTATTAAGCATGGTCATCAATGGCAGAATGATTATGAACTTCATTTAAACCACAAGAAGAAGACGGAAGAAGATAAGTTTCATAATTATTATGATTGTTGTTGCTGCTACTACTACTTAGGGTTTGTGAAAAGATTTCTCCAATAATATGGCTTTTTTATTTTTAATTTGTATTTGTATATTTCATCGC

Coding sequence (CDS)

ATGGCCTTCTCTAAAACTCTCTCACTTTCTCTCTACATTTTCTTCCCATGTTTTCTCTCTCTCTCTCAAGCCTATACTTTCTACGTCGGTGGAAAAGATGGTTGGGTTCTCAATCCCTCTGAGAGCTACGACAATTGGGCTAATCGAAACAGGTTTAGAGTCAATGACGTTCTAGTGTTCAATTATGCAAGAGGGTCGGATTCTGTGGCGGTGGTTGGTAAAGAGGATTATGATAAATGTGATTTGAATAATCCAATAGTGAAATTGGAAGATGGGAATTCGAAGTTCAAATTTGATCGTTCGGGAGCTTTTTATTTTGCTAGCGGGAAACAGGGGATGTGTGAAAATGGCCAGAAATTGGCGGTGGTTGTTATCTCTCAGCACTCTTTTTCTCTGTCCTCCAAACTGGCCTCCACGCCGCCAGAGATTTCACCTACTTCGCCACTGTCGTTGTCAGAGACACTCGGTTCGCCTATGCCCTCGTCAGAGATGCTCGGTTCCCCAATGCCCTCGTCAGAGACACTCGGCTCGCCAATGCCCTCGTCAGAGACACTCGGTTCGCCAATGCCCTCGTCAGAGACACTCAGTTCGTCATTACCGTCATCAGAGACACTCAGTTCTCCATTGCCATTGTCAGAGACGCTCGATCAGGACCCGACCCCATCCGATGCGACGGGACAGGCGACGAATCCTACCCCGTCCGATCAGACAGGAGAAATGACGGATTCGACCCCGTCCGATCCAACGGGGGAGTCGACGGATCCGACCCCCTCCGATCAGACAGGGGAGCCGACGACGTCTCTATCATTCGAGGCGTCCGGTTCAGAGTCGCCGATGTCTGACACACAGGGTTCAGAGGCACAATCGTCCGAGACAACTGATTCTTATTATGGAGAAAGCTCTTCGTCAGGCTCATACAGTTCTTCGTCTCCTTATGATATGTCGTCAGGGGCAGAACCCTCTACTTTGACACCAATCTCTCCATCGGCATCACCTTCTTCACCAGCGGAGGAGGGAGGAGAGCCACCAGCGGAGGAGGGGGGAGAGCCACCAGCGGAAGAGGGAGGAGAGCCACCAGCAGAGGAGGGATCAGAAGAGCCACCAGCGGACGAGGGAGGAGAGGACTCGTTGGCCATAGCTTTATCTCCGTCAATAAATTTGTTGGGTTTCATTACAGGTTTATTAAGCATGGTCATCAATGGCAGAATGATTATGAACTTCATTTAA

Protein sequence

MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVFNYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQATNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPISPSASPSSPAEEGGEPPAEEGGEPPAEEGGEPPAEEGSEEPPADEGGEDSLAIALSPSINLLGFITGLLSMVINGRMIMNFI*
Homology
BLAST of CSPI04G23390 vs. ExPASy Swiss-Prot
Match: Q9T076 (Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 6.8e-24
Identity = 118/342 (34.50%), Postives = 156/342 (45.61%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYT------FYVGGKDGWVLNPSESYDNWANRNRFRV 60
           M F K  SLS   FF   LSLS  +T      F VGG   WV NP E+Y++W+ +NRF V
Sbjct: 1   MTFLKMKSLS--FFFTILLSLSTLFTISNARKFNVGGSGAWVTNPPENYESWSGKNRFLV 60

Query: 61  NDVLVFNYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMC 120
           +D L F+YA+G+DSV  V K DYD C+  NPI +++DG+S+   DR G FYF SG +  C
Sbjct: 61  HDTLYFSYAKGADSVLEVNKADYDACNTKNPIKRVDDGDSEISLDRYGPFYFISGNEDNC 120

Query: 121 ENGQKLAVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSET 180
           + GQKL VVVIS    S +    +  P  S    ++      SP  SS +  +  P   T
Sbjct: 121 KKGQKLNVVVISARIPSTAQSPHAAAPGSSTPGSMTPPGGAHSPKSSSPVSPTTSPPGST 180

Query: 181 L---GSPMPSSETLGSPM---PSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQA 240
               G+  P S +  SP    P S    S  P S T S P P   T    P+ +  T   
Sbjct: 181 TPPGGAHSPKSSSAVSPATSPPGSMAPKSGSPVSPTTSPPAPPKSTSPVSPSSAPMTSPP 240

Query: 241 TNPTPSDQTGEMTDSTP-SDPTGESTDPTPSDQTGEPTTSLSFEASG--SESPMSDTQGS 300
               P   +     S P + P G     + S  +  PT S S    G  S SP     GS
Sbjct: 241 APMAPKSSSTIPPSSAPMTSPPGSMAPKSSSPVSNSPTVSPSLAPGGSTSSSPSDSPSGS 300

Query: 301 EAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPIS 328
               S    S  G+ S+     + +P    S A   T+  I+
Sbjct: 301 AMGPSGDGPSAAGDIST----PAGAPGQKKSSANGMTVMSIT 336

BLAST of CSPI04G23390 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 4.4e-23
Identity = 71/156 (45.51%), Postives = 87/156 (55.77%), Query Frame = 0

Query: 8   SLSLYIFFPCFL-SLSQAYTFYVGGKDG-WVLNPSESYD--NWANRNRFRVNDVLVFNYA 67
           SL + IF   FL SL+ A    VGGK G W + PS SY    WA + RF+V D +VF Y 
Sbjct: 11  SLHVAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYE 70

Query: 68  RGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAVV 127
            G DSV  V KE Y+ C+  NP+    DG +K K DRSG FYF SG  G CE GQKL++V
Sbjct: 71  SGKDSVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLV 130

Query: 128 VISQHSFSLSSKLASTPPEISPT---SPLSLSETLG 157
           VIS     +S   +    E  P    +P+S S  LG
Sbjct: 131 VISPRHSVISPAPSPVEFEDGPALAPAPISGSVRLG 166

BLAST of CSPI04G23390 vs. ExPASy Swiss-Prot
Match: Q5JNJ5 (Early nodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ENODL1 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 4.1e-21
Identity = 80/216 (37.04%), Postives = 110/216 (50.93%), Query Frame = 0

Query: 20  SLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVFNY-ARGSDSVAVVGKEDYD 79
           S S+AY FY GG+DGWV++P+ES++ WA RNRF+VND +VF +      SV  V + D+D
Sbjct: 24  SSSEAYVFYAGGRDGWVVDPAESFNYWAERNRFQVNDTIVFLHDDEVGGSVLQVTEGDFD 83

Query: 80  KCDLNNPIVKLED---GNSKFKFDRSGAFYFASGKQGMCENGQKLAVVVISQHSFSLSSK 139
            C   NP+ +LED   G S F+FDRSG F+F SG +  C+ GQKL ++V++         
Sbjct: 84  TCSTGNPVQRLEDVAAGRSVFRFDRSGPFFFISGDEDRCQKGQKLYIIVMA--------- 143

Query: 140 LASTPPEISPTSPLSLSETLGSPMPSSEMLGSPM---PSSETLGSPMPSSETLGSPMPSS 199
                  + PT P    E  G+  P S    S     P+  T   P+P S    +P  + 
Sbjct: 144 -------VRPTKPSEAPEPAGAAGPVSSKSWSWQAFPPAGATTPPPLPPSWG-SAPEHAQ 203

Query: 200 ETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQA 229
               SSL  S      +  S +L   P  S A G A
Sbjct: 204 APGKSSLGGSG--GGEMSRSSSLGAPPPTSGAAGLA 220

BLAST of CSPI04G23390 vs. ExPASy Swiss-Prot
Match: Q8LC95 (Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 2.6e-15
Identity = 62/185 (33.51%), Postives = 93/185 (50.27%), Query Frame = 0

Query: 5   KTLSLSLYIFFPCFLSLSQAYTFYVGGK-DGWVL--NPSESYDNWANRNRFRVNDVLVFN 64
           +TL  + ++ F    +L  +    VGGK   W +  +PSES + WA   RFRV D LV+ 
Sbjct: 4   RTLVATFFLIFFLLTNLVCSKEIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWK 63

Query: 65  YARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLA 124
           Y    DSV  V K+ Y  C+  NP     +G++K K +RSG ++F SG +  C  G+KL 
Sbjct: 64  YDEEKDSVLQVTKDAYINCNTTNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLH 123

Query: 125 VVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMPS 184
           +VV       +SS+   T    + +SP        SP PS  +LG+P  +  + GS    
Sbjct: 124 IVV-------MSSRGGHTGGFFTGSSP--------SPAPSPALLGAPTVAPASGGSASSL 173

Query: 185 SETLG 187
           +  +G
Sbjct: 184 TRQVG 173

BLAST of CSPI04G23390 vs. ExPASy Swiss-Prot
Match: P93329 (Early nodulin-20 OS=Medicago truncatula OX=3880 GN=ENOD20 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.9e-14
Identity = 82/261 (31.42%), Postives = 122/261 (46.74%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDG---WVLNPSESYDNWANRNRFRVNDV 60
           M+ S  + L         +S S++  + VG  +    + L    +   WA+  +F V D 
Sbjct: 1   MSSSSPILLMFIFSIWMLISYSESTDYLVGDSENSWKFPLPTRHALTRWASNYQFIVGDT 60

Query: 61  LVFNYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENG 120
           + F Y   ++SV  V +EDYD+C +    V   DGN+     ++G  +F SGK+  C  G
Sbjct: 61  ITFQYNNKTESVHEVEEEDYDRCGIRGEHVDHYDGNTMVVLKKTGIHHFISGKKRHCRLG 120

Query: 121 QKLAVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGS 180
            KLAVVV+      ++  L+S PP  SP +P S      +P+P       P P      S
Sbjct: 121 LKLAVVVM------VAPVLSSPPPPPSPPTPRS-----STPIPHPPRRSLPSPP-----S 180

Query: 181 PMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQATNPTPSDQT 240
           P PS     SP PS  +     P   + +SP P S +L + P+PS++   A  P+PSD  
Sbjct: 181 PSPSPSPSPSPSPSPRSTPIPHPRKRSPASPSP-SPSLSKSPSPSESPSLA--PSPSDSV 240

Query: 241 GEMTDSTPSDPTGESTDPTPS 259
             +  S  S P+ ES  P PS
Sbjct: 241 ASLAPS--SSPSDESPSPAPS 240

BLAST of CSPI04G23390 vs. ExPASy TrEMBL
Match: A0A1S4DZ91 (skin secretory protein xP2-like OS=Cucumis melo OX=3656 GN=LOC103493884 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 2.1e-124
Identity = 314/500 (62.80%), Postives = 338/500 (67.60%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVLVF
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVLVF 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
            Y RGSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  KYPRGSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP--------------------------------------------------PAE 405
           + E G P                                                  PA 
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAP 420

BLAST of CSPI04G23390 vs. ExPASy TrEMBL
Match: A0A5D3D9M8 (Skin secretory protein xP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004050 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 8.1e-121
Identity = 310/493 (62.88%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVL  
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVL-- 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
               GSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  ----GSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP-------------------------------------------PAEEG----- 405
           + E G P                                           PA EG     
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPEGEAPAP 420

BLAST of CSPI04G23390 vs. ExPASy TrEMBL
Match: A0A5A7VF32 (Skin secretory protein xP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00200 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 5.3e-120
Identity = 310/500 (62.00%), Postives = 334/500 (66.80%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVL  
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVL-- 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
               GSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  ----GSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP--------------------------------------------------PAE 405
           + E G P                                                  PA 
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAP 420

BLAST of CSPI04G23390 vs. ExPASy TrEMBL
Match: A0A0A0KZL9 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G632110 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.9e-85
Identity = 190/263 (72.24%), Postives = 210/263 (79.85%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF
Sbjct: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
           NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL
Sbjct: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGS--- 180
           AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSE LGS   
Sbjct: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSEMLGSFVI 180

Query: 181 -PMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQATNPTPSDQ 240
             +  ++ + + +  ++ + + +  ++  S  +        DP     TG  + P  SD+
Sbjct: 181 TIIRDTQFVITIIRDTQFVITIIRDTQ-FSIAIVRDARSGPDPIRCHGTGDESYPVRSDR 240

Query: 241 TGEMTDSTPSDPTGESTDPTPSD 260
             +  D   S+   + +DP  SD
Sbjct: 241 RNDGFDPVRSNGGVDGSDPLRSD 262

BLAST of CSPI04G23390 vs. ExPASy TrEMBL
Match: A0A6J1F7K0 (early nodulin-like protein 2 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111442818 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.4e-48
Identity = 209/531 (39.36%), Postives = 255/531 (48.02%), Query Frame = 0

Query: 13  IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVFNYARGSDSVAVV 72
           +FFPCFLS+S AYTFY GGKDGWVLNPSESYDNW++RNRF+VNDV+VF YA+G DSV VV
Sbjct: 10  LFFPCFLSVSHAYTFYAGGKDGWVLNPSESYDNWSHRNRFQVNDVIVFKYAKGLDSVLVV 69

Query: 73  GKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAVVVISQH---- 132
            KE Y+KCDL NPI+KLEDGNS+FKFDRSG FYF SGKQG CE GQKL VVV++Q     
Sbjct: 70  SKEGYEKCDLKNPIMKLEDGNSEFKFDRSGPFYFVSGKQGSCEKGQKLMVVVLAQRPPPP 129

Query: 133 -------------SFS---LSSKLASTPPEISPT------------------SPLSLSET 192
                        S+S   L S   + PP +SP+                  +P+S S  
Sbjct: 130 YTVGAPPPSGAAPSYSPPVLPSPPQAKPPGVSPSPPSGSPGAKSPSGSPPSATPISPSPP 189

Query: 193 LGSP-MPSSEMLGSPMPS----------------SETLGSPMPSSETLGSPMPSSETLSS 252
            G+P  PS     SP PS                S T  SP PSS T   P P S T SS
Sbjct: 190 SGTPTSPSPSGSHSPAPSGSHSPGGAKSPSGSPPSGTPSSPSPSSGTPILPSPPSGTPSS 249

Query: 253 SLPS---------SETLSSPLPLSETLDQDP------TPSDATGQATNPTPSDQT----- 312
             PS         S +  SP P +++    P      +PS ++G    P+P   T     
Sbjct: 250 PSPSGSHAPVPSGSHSPGSPYPGAKSPSGSPPSGTPSSPSPSSGTPILPSPPSGTPSSPS 309

Query: 313 --GEMTDSTPSDPTGESTDPTPSDQT-------GEPTTSLSFEASGSESPMSDTQGSEAQ 372
             G  T  T   P G  T P P   T       G P T+ +   S S SP + T  S + 
Sbjct: 310 PSGSETPVTTPVPAGSPTSPLPGTPTSPSPSGSGTPVTTPTPAGSPSSSPGTPTSPSPSG 369

Query: 373 SS-ETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPI-------------SPSASP-- 408
           S+   T  Y G SS SGS+ S SP    SG+E    +P+             SPS SP  
Sbjct: 370 SAGSPTSPYPGASSPSGSHISPSP----SGSETPITSPVPAGSPTSTIPGATSPSGSPLP 429

BLAST of CSPI04G23390 vs. NCBI nr
Match: XP_004141358.3 (early nodulin-like protein 2 [Cucumis sativus] >KAE8649838.1 hypothetical protein Csa_012805 [Cucumis sativus])

HSP 1 Score: 681.0 bits (1756), Expect = 6.3e-192
Identity = 408/520 (78.46%), Postives = 408/520 (78.46%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF
Sbjct: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
           NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL
Sbjct: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSET--------------------LGSPMP 180
           AVVVISQHSFSLSSKLASTPPEISPTSPLSLSET                    LGSPMP
Sbjct: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSEMLGSPMP 180

Query: 181 SSEMLGSPMPSSETLGSPMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPT 240
           SSEMLGSPMPSSETLGSPMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPT
Sbjct: 181 SSEMLGSPMPSSETLGSPMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPT 240

Query: 241 PSDATGQATNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMS 300
           PSDATGQATNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMS
Sbjct: 241 PSDATGQATNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMS 300

Query: 301 DTQGSEAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPISPSASPSS------ 360
           DTQGSEAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPISPSASPSS      
Sbjct: 301 DTQGSEAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPISPSASPSSAPAEGG 360

Query: 361 ----------------PAEEGGEPPAEEGGEPPAEEGG---------------------- 409
                           PAEEGGEPPAEEGGEPPAEEGG                      
Sbjct: 361 EPPAEGGEPPAEGGEPPAEEGGEPPAEEGGEPPAEEGGEPPAEEEGEPPAEEGGEPPAEE 420

BLAST of CSPI04G23390 vs. NCBI nr
Match: XP_016901288.1 (PREDICTED: skin secretory protein xP2-like [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 4.3e-124
Identity = 314/500 (62.80%), Postives = 338/500 (67.60%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVLVF
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVLVF 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
            Y RGSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  KYPRGSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP--------------------------------------------------PAE 405
           + E G P                                                  PA 
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAP 420

BLAST of CSPI04G23390 vs. NCBI nr
Match: TYK20265.1 (skin secretory protein xP2-like [Cucumis melo var. makuwa])

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-120
Identity = 310/493 (62.88%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVL  
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVL-- 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
               GSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  ----GSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP-------------------------------------------PAEEG----- 405
           + E G P                                           PA EG     
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPEGEAPAP 420

BLAST of CSPI04G23390 vs. NCBI nr
Match: KAA0064321.1 (skin secretory protein xP2-like [Cucumis melo var. makuwa])

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-119
Identity = 310/500 (62.00%), Postives = 334/500 (66.80%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFSKTLSLSL IFFPCFLSLSQAYTFYVGGKDGWVLNPSESYD WANRN+FRVNDVL  
Sbjct: 1   MAFSKTLSLSLCIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDIWANRNKFRVNDVL-- 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
               GSDSVAVVGKEDYDKCDLNNPI+KLEDGNS+FKFDRSGAFYFASGK+G CENGQK+
Sbjct: 61  ----GSDSVAVVGKEDYDKCDLNNPIMKLEDGNSEFKFDRSGAFYFASGKRGKCENGQKM 120

Query: 121 AVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 180
            VVV+SQ SFSLSSKLASTPPEISPT PLS S+TLGSPMPSS+ LGSPMPSS+TLGSPMP
Sbjct: 121 VVVVMSQRSFSLSSKLASTPPEISPTLPLSSSDTLGSPMPSSDTLGSPMPSSDTLGSPMP 180

Query: 181 SSETLGSPMPSSETLSSSLPSSETL------------SSPLPLSETLDQDPTPSDATGQA 240
           SS+TLGSP+PSS+TL S LPSSETL              P P  +TLDQDPTPSD     
Sbjct: 181 SSDTLGSPLPSSDTLGSPLPSSETLDQDPNSFDQTLDQDPTPSDQTLDQDPTPSDQMLDQ 240

Query: 241 TNPTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQ 300
             PTPSDQT +  D TPSD T  + DPTPSD TG+  TS SF+ASGSE PMS+T  SEAQ
Sbjct: 241 A-PTPSDQTLDQ-DPTPSDQT-LNQDPTPSDSTGD--TSQSFQASGSEQPMSETMNSEAQ 300

Query: 301 SSETTDSYYGESSSSGSYSSSSPYDMSSGAEPS--TLTPISPSASP-----------SSP 360
           SS+T+ SYYG SSSS SY SSSPYD S G EPS  TLTPI+PS SP           SSP
Sbjct: 301 SSDTSGSYYGGSSSSDSYGSSSPYD-SGGTEPSTGTLTPIAPSVSPSSATTPGTSPASSP 360

Query: 361 AEEGGEP--------------------------------------------------PAE 405
           + E G P                                                  PA 
Sbjct: 361 SSESGSPAKSPEGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAPAGEAPAP 420

BLAST of CSPI04G23390 vs. NCBI nr
Match: XP_038896399.1 (early nodulin-like protein 2 [Benincasa hispida])

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-58
Identity = 197/385 (51.17%), Postives = 230/385 (59.74%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVF 60
           MAFS++LSLSL IF PCFLSLSQAYTFYVGGKDGWVL+PSESYDNWANRNRF+VNDVLVF
Sbjct: 1   MAFSRSLSLSLCIFLPCFLSLSQAYTFYVGGKDGWVLDPSESYDNWANRNRFQVNDVLVF 60

Query: 61  NYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKL 120
            YARGSDSV VV KEDYDKCDLNNP+VKLEDGNS+FKFDRSGAFYF SGK G CENGQKL
Sbjct: 61  KYARGSDSVLVVSKEDYDKCDLNNPVVKLEDGNSEFKFDRSGAFYFVSGKPGSCENGQKL 120

Query: 121 AVVVISQHSFSLSSKLAS------TPPEISPTSP--LSLSETLGSPMPSSEMLG--SPMP 180
            VVV+S  S    SK+ S       PPE+SP+ P   S S T  SP PS       +P+P
Sbjct: 121 VVVVMSLRSLPPPSKVGSPPPYGAQPPEVSPSPPGAESPSRTPTSPSPSGATSPGMAPVP 180

Query: 181 SSETLGSPMPSSETLGSPMPSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQATN 240
            S +     P SET  S  PSS T  SS PSS T SSP P S T    P+PS ++   T+
Sbjct: 181 PSAS-----PPSETPSSSSPSSGT-PSSPPSSGTPSSPSPSSMT-PTSPSPSPSSLTPTS 240

Query: 241 PTPSDQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQSS 300
           P+PS  T  +       PT  S   + S ++  P     +   GS       +   A S 
Sbjct: 241 PSPSPGTPTLLYPPSETPTSPSPSESYSPRSSYPGAQSPY---GSPPYGYGYETPPAASP 300

Query: 301 ETTDSYYGESSSSGSYSSSSP--YDMSSGAEPST----LTPISPSASPSSPAEEGGEPPA 360
            +   Y G  S S SY+S SP  Y+    A P++        SPSAS +SP+  G E P 
Sbjct: 301 TSGHGYPGARSPSASYTSPSPSGYETPPAASPTSGYGYPGARSPSASYTSPSPSGYETPT 360

Query: 361 EEGGEPPAEEGGEPPAEEGSEEPPA 370
                PPA   G P +    E  P+
Sbjct: 361 T---TPPA---GSPTSPSPGEISPS 369

BLAST of CSPI04G23390 vs. TAIR 10
Match: AT4G32490.1 (early nodulin-like protein 4 )

HSP 1 Score: 125.9 bits (315), Expect = 7.2e-29
Identity = 79/186 (42.47%), Postives = 103/186 (55.38%), Query Frame = 0

Query: 19  LSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVFNYARGSDSVAVVGKEDYD 78
           + LS  + FYVGG+DGWVL PSE Y +W++RNRF+VND L F Y +G DSV  V +++Y+
Sbjct: 23  IELSNGHKFYVGGRDGWVLTPSEDYSHWSHRNRFQVNDTLYFKYVKGKDSVLEVSEKEYN 82

Query: 79  KCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAVVVIS--QHSFSLSSKL 138
            C+  +P+  L DG+S F   RS  F+F SG  G C  GQKLAV V+S   HS +     
Sbjct: 83  TCNTTHPLTSLSDGDSLFLLSRSDPFFFVSGNSGSCLKGQKLAVTVMSTGHHSHTPRHPS 142

Query: 139 ASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMPSSETLGSPMPSSETLS 198
            S  P  SP     LS    +P+P  + L SP P   T G     SE L +P P      
Sbjct: 143 PSPSPSASPVRKALLSP---APIPVHKALSSPAP---TPGVDPSHSEVL-APAPGPAAAV 201

Query: 199 SSLPSS 203
            +L  S
Sbjct: 203 RNLAGS 201

BLAST of CSPI04G23390 vs. TAIR 10
Match: AT4G28365.1 (early nodulin-like protein 3 )

HSP 1 Score: 119.4 bits (298), Expect = 6.8e-27
Identity = 80/199 (40.20%), Postives = 110/199 (55.28%), Query Frame = 0

Query: 7   LSLSLYIFFPCFLSL----SQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLVFNY 66
           +   LY+ F   + L    S  Y FYVGGKDGWV  PSE Y +W++RNRF+VND L F Y
Sbjct: 5   MRFDLYLMFVMLMGLGFTISNGYKFYVGGKDGWVPTPSEDYSHWSHRNRFQVNDTLHFKY 64

Query: 67  ARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAV 126
           A+G DSV  V +++Y+ C+  +P+  L DG+S F    SG+++F SG    C  GQKLAV
Sbjct: 65  AKGKDSVLEVTEQEYNTCNTTHPLTSLSDGDSLFLLSHSGSYFFISGNSQNCLKGQKLAV 124

Query: 127 VVIS--QHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPMP 186
            V+S   HS S        P   SP SP  + + L SP PS  +     PSS+       
Sbjct: 125 KVLSTVHHSHS--------PRHTSP-SPSPVHQELSSPGPSPGV----EPSSD------- 183

Query: 187 SSETLGSPMPSSETLSSSL 200
           S+  + +P P++   S+ L
Sbjct: 185 SNSRVPAPGPATAPNSAGL 183

BLAST of CSPI04G23390 vs. TAIR 10
Match: AT5G53870.1 (early nodulin-like protein 1 )

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-26
Identity = 125/350 (35.71%), Postives = 168/350 (48.00%), Query Frame = 0

Query: 2   AFSKTLSLSLYIF--FPCFLSLSQAYTFYVGGKDGWVLNPSESYDNWANRNRFRVNDVLV 61
           A  K+L  S  I   F  F S++ A+ F VGG   WV NP E+Y+ WA RNRF+VND L 
Sbjct: 3   AIMKSLCFSFLILASFATFFSVADAWRFNVGGNGAWVTNPQENYNTWAERNRFQVNDSLY 62

Query: 62  FNYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQK 121
           F YA+GSDSV  V K D+D C++ NPI   E+G S    DRSGAFYF SG Q  C+ GQK
Sbjct: 63  FKYAKGSDSVQQVMKADFDGCNVRNPIKNFENGESVVTLDRSGAFYFISGNQDHCQKGQK 122

Query: 122 LAVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSETLGSPM 181
           L VVV++  +   S+   S  P +SPT P      +    P+S    S  P S    +  
Sbjct: 123 LIVVVLAVRN-QPSAPAHSPVPSVSPTQPPKSHSPVSPVAPASAPSKSQPPRSSVSPAQP 182

Query: 182 PSSETLGSPMPSSETLSSSLPSSETLSSPLPLSET---LDQDPTPSDATGQATNP--TPS 241
           P S    SP+  +  LS S  +S + ++P P  ++   +   P+ S A   + +P  TPS
Sbjct: 183 PKS---SSPISHTPALSPSHATSHSPATPSPSPKSPSPVSHSPSHSPAHTPSHSPAHTPS 242

Query: 242 DQTGEMTDSTPSDPTGESTDPTPSDQTGEPTTSLSFEASGSESPMSDTQGSEAQSSETTD 301
                    +P+     S    PS     P  S S   +  +SP      S AQS  T  
Sbjct: 243 HSPAHAPSHSPAHAPSHSPAHAPSH---SPAHSPSHSPATPKSP--SPSSSPAQSPATPS 302

Query: 302 SYYGESSSSGSYSSSSPYDMSSGAEPSTLTPISPSASPSSPAEEGGEPPA 345
               +S S      SSP    S A     TP++PS S ++P  +    PA
Sbjct: 303 PMTPQSPS----PVSSPSPDQSAAPSDQSTPLAPSPSETTPTADNITAPA 339

BLAST of CSPI04G23390 vs. TAIR 10
Match: AT4G27520.1 (early nodulin-like protein 2 )

HSP 1 Score: 113.2 bits (282), Expect = 4.8e-25
Identity = 118/342 (34.50%), Postives = 156/342 (45.61%), Query Frame = 0

Query: 1   MAFSKTLSLSLYIFFPCFLSLSQAYT------FYVGGKDGWVLNPSESYDNWANRNRFRV 60
           M F K  SLS   FF   LSLS  +T      F VGG   WV NP E+Y++W+ +NRF V
Sbjct: 1   MTFLKMKSLS--FFFTILLSLSTLFTISNARKFNVGGSGAWVTNPPENYESWSGKNRFLV 60

Query: 61  NDVLVFNYARGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMC 120
           +D L F+YA+G+DSV  V K DYD C+  NPI +++DG+S+   DR G FYF SG +  C
Sbjct: 61  HDTLYFSYAKGADSVLEVNKADYDACNTKNPIKRVDDGDSEISLDRYGPFYFISGNEDNC 120

Query: 121 ENGQKLAVVVISQHSFSLSSKLASTPPEISPTSPLSLSETLGSPMPSSEMLGSPMPSSET 180
           + GQKL VVVIS    S +    +  P  S    ++      SP  SS +  +  P   T
Sbjct: 121 KKGQKLNVVVISARIPSTAQSPHAAAPGSSTPGSMTPPGGAHSPKSSSPVSPTTSPPGST 180

Query: 181 L---GSPMPSSETLGSPM---PSSETLSSSLPSSETLSSPLPLSETLDQDPTPSDATGQA 240
               G+  P S +  SP    P S    S  P S T S P P   T    P+ +  T   
Sbjct: 181 TPPGGAHSPKSSSAVSPATSPPGSMAPKSGSPVSPTTSPPAPPKSTSPVSPSSAPMTSPP 240

Query: 241 TNPTPSDQTGEMTDSTP-SDPTGESTDPTPSDQTGEPTTSLSFEASG--SESPMSDTQGS 300
               P   +     S P + P G     + S  +  PT S S    G  S SP     GS
Sbjct: 241 APMAPKSSSTIPPSSAPMTSPPGSMAPKSSSPVSNSPTVSPSLAPGGSTSSSPSDSPSGS 300

Query: 301 EAQSSETTDSYYGESSSSGSYSSSSPYDMSSGAEPSTLTPIS 328
               S    S  G+ S+     + +P    S A   T+  I+
Sbjct: 301 AMGPSGDGPSAAGDIST----PAGAPGQKKSSANGMTVMSIT 336

BLAST of CSPI04G23390 vs. TAIR 10
Match: AT2G25060.1 (early nodulin-like protein 14 )

HSP 1 Score: 110.5 bits (275), Expect = 3.1e-24
Identity = 71/156 (45.51%), Postives = 87/156 (55.77%), Query Frame = 0

Query: 8   SLSLYIFFPCFL-SLSQAYTFYVGGKDG-WVLNPSESYD--NWANRNRFRVNDVLVFNYA 67
           SL + IF   FL SL+ A    VGGK G W + PS SY    WA + RF+V D +VF Y 
Sbjct: 11  SLHVAIFSLIFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYE 70

Query: 68  RGSDSVAVVGKEDYDKCDLNNPIVKLEDGNSKFKFDRSGAFYFASGKQGMCENGQKLAVV 127
            G DSV  V KE Y+ C+  NP+    DG +K K DRSG FYF SG  G CE GQKL++V
Sbjct: 71  SGKDSVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLV 130

Query: 128 VISQHSFSLSSKLASTPPEISPT---SPLSLSETLG 157
           VIS     +S   +    E  P    +P+S S  LG
Sbjct: 131 VISPRHSVISPAPSPVEFEDGPALAPAPISGSVRLG 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0766.8e-2434.50Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 S... [more]
Q9SK274.4e-2345.51Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S... [more]
Q5JNJ54.1e-2137.04Early nodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ENODL1 ... [more]
Q8LC952.6e-1533.51Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 S... [more]
P933294.9e-1431.42Early nodulin-20 OS=Medicago truncatula OX=3880 GN=ENOD20 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DZ912.1e-12462.80skin secretory protein xP2-like OS=Cucumis melo OX=3656 GN=LOC103493884 PE=4 SV=... [more]
A0A5D3D9M88.1e-12162.88Skin secretory protein xP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5A7VF325.3e-12062.00Skin secretory protein xP2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A0A0KZL91.9e-8572.24Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G632110... [more]
A0A6J1F7K01.4e-4839.36early nodulin-like protein 2 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_004141358.36.3e-19278.46early nodulin-like protein 2 [Cucumis sativus] >KAE8649838.1 hypothetical protei... [more]
XP_016901288.14.3e-12462.80PREDICTED: skin secretory protein xP2-like [Cucumis melo][more]
TYK20265.11.7e-12062.88skin secretory protein xP2-like [Cucumis melo var. makuwa][more]
KAA0064321.11.1e-11962.00skin secretory protein xP2-like [Cucumis melo var. makuwa][more]
XP_038896399.11.4e-5851.17early nodulin-like protein 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G32490.17.2e-2942.47early nodulin-like protein 4 [more]
AT4G28365.16.8e-2740.20early nodulin-like protein 3 [more]
AT5G53870.11.2e-2635.71early nodulin-like protein 1 [more]
AT4G27520.14.8e-2534.50early nodulin-like protein 2 [more]
AT2G25060.13.1e-2445.51early nodulin-like protein 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 35..118
e-value: 2.5E-19
score: 69.1
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 25..126
score: 38.288048
IPR008972CupredoxinGENE3D2.60.40.420coord: 23..130
e-value: 6.3E-33
score: 115.1
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 342..372
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..377
NoneNo IPR availablePANTHERPTHR33021:SF404EARLY NODULIN-LIKE PROTEIN 2coord: 1..223
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 1..223
IPR041846Early nodulin-like protein domainCDDcd11019OsENODL1_likecoord: 25..125
e-value: 2.92366E-49
score: 160.511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G23390.1CSPI04G23390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0009055 electron transfer activity