CSPI04G22430 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G22430
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr4: 20717732 .. 20721796 (-)
RNA-Seq Expression	CSPI04G22430
Synteny	CSPI04G22430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCACGTAATTAATTTTGTATAATAATAAATAACAAATATACAAACATCCAAAAATATGAATGTGCTGAAAAGCATAGAAATCAAAACCAAATCTCATTACCATTTTCTTTCATACACACATTTAGAAACCAATCAATCAGAAAAATAAGAAACACATGGCGGAGGAGAAGAAGATCCACACGTTAAGGTTCGGAAGTTCCATTCTAGTTCCTTCCGTTCAAGAGTTAGCCAAACACCCCATCACCGAAATTCCACATCGATACATCCGCCCCGATCTCCTCCACCACTCCCCGATCGCCTCCGCCGCTTCCCAAATCCCCGTGATCGACATGTCCAACTTCCGCTCTAATGATACAATGGACTCCGAATTGTCTCGCCTTCACTCCGCCTGTAAAAATTGGGGATTCTTTCAGGTTCATAATATCTCCTCTCTCTATCTCTGTTTCTATGTGTTTAATTAATAAGTAGAATCTGAAAATGATTGGTAAAATTAATTACAGTTGGTGAAGCATGGAGTGAGCGATTCATTGATGGAGAGAATGAAGATGGAAACGCAGAAATTGTTCCAACTTCCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAAGGGGATGTGGAGGGGTTTGGACAAGCTTTCATTACAAGTGAAGAACAGAAATTGGATTGGTGTGATATTTTCTTCATTGCTACATCGCCTTTACATTTCAGGAATCCCCGATTGTTTCAAAATCTCCCTCTCTCTTTGAGGTTTGATAACTCCTTCCATCACAAAACTTTTTAAATGATTCTCAAACTCATATGAATCCTAGAAATTGAACTCCGAACTCATAAAAATTAAACCAACCAACCTATAAAATTTTTCTAAGCAAAGATTAATCTTGTAATTTAAGCAAGTGAGAATAAAAAGAAGTAATCCATTATAGGTTAAAACAAAGTTCATTAGTTTAATTGATTTTAAAAAAAAGAATTAGGAAATAATTATGAAACTGTATTGGTAAGCAGAGAAACATTGGAAGAGTACTCGGCGGCGGTGAAGAATGTGACGACGGCGATAATTGGCGGCATTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGATGAGTGAGTTGTTCAAAGACGGTAATCAATCGATGAGAATAAACTATTACCCGCGGTGCCCGGAGCCGGAGAAGGTGATCGGACTGACGCCTCATTCCGACTCGGTTGGGCTAACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGAAGGAAATTGGATTACCGTTATGCCCCTTCCAAATGCCTTCATCGTTAACATTGGCGACATCTTGGAGGTAATTTTACACTCCCCTCTCATGCACTTCTCTTTTCTAACCTAATAATATAATAAAGGCCCTTAATCTTTTAAAAAAAAATGCCTATTGAAAAATTTAATAAGTACTTTAAACTTATAAAATTTTTAAAAATATTTCCATTTATCCTATTTTTACATCAAATCTCTAAGCTCTCAAAAATAAAAATTACTAAAATTTAAATTTAAATTTCTATAAATATGTGGATATTTATATCAATATTTTTTGGGGTGAATCGATACAATAACACACAAATAAAAAGATTTTTTTTTAAAAGAACTCTATAATATAAATAATAAATAAATTTACTTATTTGAAAAATCTATATTTAATAATTTAAATTACACAAAATCTTATAAAGTTTAAAGAAAAACTCCTAAAAAAAGTATAAAAAAAATCTCACAATAATGATCAAATAAAAACAACTAGACTATTAGTGTGTTTTGGTAGTCAAATATATATTTTTATTTGACAATTAACTTAATTACAATCATAATTGTTGAATTTGGTTGTGTTCGTGATCTATTATGCTATATTCAAATAAATTTAATAATATAGTTTTAACTTAAAATTTTGATTTTGATTTTAGTTGGTGAAAAAATTGATGTCAAATTTGAATAGACGATAATATATTTTAACATTTTTTAAACTCAAGTGTATTTTTAAAACTTTTGATAGTTATTTTTGAAACAAAACTTCAATGGTTTCAATTTAAAACTATCGGTAAAATTAACTTTGAACAATTTTCAAAAGTTTAAGTGTATTTTTAAATTTTTTAAAAGTTTAGGTGTATTTTTAAAACAAATATAAAAGTTGAGGAATATTTTTTACAATTAAGCTAATGATAAAAATAAAATATTTGTCATGTAACAAATAAATGAGTATAATGTGATATGTGCATTTTTCATTTTTGTAACGGATGATACTTAGGTGTGTTTTAACATCACCCGTCTCTTTCTCGAACACTCATATTAAAAGATATAAAAATATTCTAGAAAAAAGAAAAAACAAATTTGCATCGTCTTCATGATCGGATAATTCGATGGAATAAAAGATTGGATGAAAGACAAACTCAGTTTGGTTTGTTTATTTAACTTTTTATTTAATTTAGTAGACAATCTTGATTTTAAAAACCATAAATTTCCAACCTTTTAAAATATGTGATGTCTTCGTCTCTTTGTTTTTATAATATATCTCTAACATTTTTCTTAATTTGTTAAAATATTACTCATTCTATTCTCAAAATCACTTGAACAAATTTTAATAGTATGAAAATTTTGCAAATTAAACGAATTGATTTAAGTGATTATTGATAAGTAATTGCTTGAAATGATAATTAGAGTAAAAATTGTTTTTTTTTTAAAAAAAATTAATTTTAGTAACTATTTTGACTATTTTTTTTTCTAAAAGGAATTAGGTTGGATAGTAATTCTAACCCTTATAAAACTACTCTCAAATATACATCAAAATATTAAACCATTTTGTAAATGTGAAAATTTAAATATTCAAGAGACCAAAGCTTCTTTGAAGTTACATTACTTTTATCTAATATATTTTGAAACTTATATCTATTTAGATTAGGTTTTAAACTTGTAATATTTTAAGAAAAAGTCTTTAATCATTTGGTTTAAATCACACCTAATTTGAAATTAATTAGATACAAATATGAAAAATTAAAGATTTGTTAGATAAATAGTTGGTGTTTTTAATGACTTTATCTTGAAAACACAAATATTAGTCCATTTTTTTGAAATACAAAACGTATATTAAATTTAGTTCTAAAATTGTGGTTTAACATAAACACAAGTATTTTAATTCTTAATGAGAAATTATATTTATACAATCGATTATATCATTAATGTGATTTGTCAAACACAAATAAAGTTCAACATAATTGGCGGTATATTCCTTTACTCGACTCAAGTTCTATTTGTTATACTAACAAATACTAAAAAAATGTATATTTACTTTTTGGTTTGAAGGTTTGATTGGAGTAGTAATTTGGACTATGCTTTGTTAATTCTCTACAAAATTATTAATGGAAAGATTATTTGATTGTATGAATTTTTATCTCAAAATCAATTTAGAATGGGGCGAGTAACTCATTTATTTTATAAAGAATTTGAATTCTCTTAATTGTGAATGTTTCAATTTATATCCGAGCAAAGTTGACGTTATTTACTTGTTTACCTCTATTTCTCACACTTTTTTTCTTCGAGCTTTCCAACCTTCTCCATCTGTTTTCGTCTTTGGAAAATTTTGTAGACTATATTTTCAATATTGCTTTGATTTAATAATAATTATAAGTATGGCAGATGGTAACAAATGGGAAATACAAAAGCATAGAGCATTGTGCAACAGTGAACTCAAAGAGTGAAAGGCTGTCCATAGCAACTTTCTACACTCCAAGTTTGGAGAAAGAAATAAGGCCAACACCATCCCTCATCACACCACATTCTCCTCCCTTGTTTCGAACTTTGACATATCAAGAGTATGTGAAGGGCCTTTTCTCTCGAACACTCGACGGAAAAACTTATCTCGACGCTATGCGAATTCACCCTCCTTCAACCTAACCATTGCTTTTCAACTCAAAATCTAAAAGTGTCATATGAATTCATCATTTTTCTTACTAGATTTAAATAATATATTTGAATTTGACTATTTTGGATGTTAGTGTATTATATGATTATTATTTATTCGAGTATTGTGACTTAGTA

mRNA sequence

TCTCACGTAATTAATTTTGTATAATAATAAATAACAAATATACAAACATCCAAAAATATGAATGTGCTGAAAAGCATAGAAATCAAAACCAAATCTCATTACCATTTTCTTTCATACACACATTTAGAAACCAATCAATCAGAAAAATAAGAAACACATGGCGGAGGAGAAGAAGATCCACACGTTAAGGTTCGGAAGTTCCATTCTAGTTCCTTCCGTTCAAGAGTTAGCCAAACACCCCATCACCGAAATTCCACATCGATACATCCGCCCCGATCTCCTCCACCACTCCCCGATCGCCTCCGCCGCTTCCCAAATCCCCGTGATCGACATGTCCAACTTCCGCTCTAATGATACAATGGACTCCGAATTGTCTCGCCTTCACTCCGCCTGTAAAAATTGGGGATTCTTTCAGTTGGTGAAGCATGGAGTGAGCGATTCATTGATGGAGAGAATGAAGATGGAAACGCAGAAATTGTTCCAACTTCCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAAGGGGATGTGGAGGGGTTTGGACAAGCTTTCATTACAAGTGAAGAACAGAAATTGGATTGGTGTGATATTTTCTTCATTGCTACATCGCCTTTACATTTCAGGAATCCCCGATTGTTTCAAAATCTCCCTCTCTCTTTGAGAGAAACATTGGAAGAGTACTCGGCGGCGGTGAAGAATGTGACGACGGCGATAATTGGCGGCATTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGATGAGTGAGTTGTTCAAAGACGGTAATCAATCGATGAGAATAAACTATTACCCGCGGTGCCCGGAGCCGGAGAAGGTGATCGGACTGACGCCTCATTCCGACTCGGTTGGGCTAACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGAAGGAAATTGGATTACCGTTATGCCCCTTCCAAATGCCTTCATCGTTAACATTGGCGACATCTTGGAGATGGTAACAAATGGGAAATACAAAAGCATAGAGCATTGTGCAACAGTGAACTCAAAGAGTGAAAGGCTGTCCATAGCAACTTTCTACACTCCAAGTTTGGAGAAAGAAATAAGGCCAACACCATCCCTCATCACACCACATTCTCCTCCCTTGTTTCGAACTTTGACATATCAAGAGTATGTGAAGGGCCTTTTCTCTCGAACACTCGACGGAAAAACTTATCTCGACGCTATGCGAATTCACCCTCCTTCAACCTAACCATTGCTTTTCAACTCAAAATCTAAAAGTGTCATATGAATTCATCATTTTTCTTACTAGATTTAAATAATATATTTGAATTTGACTATTTTGGATGTTAGTGTATTATATGATTATTATTTATTCGAGTATTGTGACTTAGTA

Coding sequence (CDS)

ATGGCGGAGGAGAAGAAGATCCACACGTTAAGGTTCGGAAGTTCCATTCTAGTTCCTTCCGTTCAAGAGTTAGCCAAACACCCCATCACCGAAATTCCACATCGATACATCCGCCCCGATCTCCTCCACCACTCCCCGATCGCCTCCGCCGCTTCCCAAATCCCCGTGATCGACATGTCCAACTTCCGCTCTAATGATACAATGGACTCCGAATTGTCTCGCCTTCACTCCGCCTGTAAAAATTGGGGATTCTTTCAGTTGGTGAAGCATGGAGTGAGCGATTCATTGATGGAGAGAATGAAGATGGAAACGCAGAAATTGTTCCAACTTCCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAAGGGGATGTGGAGGGGTTTGGACAAGCTTTCATTACAAGTGAAGAACAGAAATTGGATTGGTGTGATATTTTCTTCATTGCTACATCGCCTTTACATTTCAGGAATCCCCGATTGTTTCAAAATCTCCCTCTCTCTTTGAGAGAAACATTGGAAGAGTACTCGGCGGCGGTGAAGAATGTGACGACGGCGATAATTGGCGGCATTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGATGAGTGAGTTGTTCAAAGACGGTAATCAATCGATGAGAATAAACTATTACCCGCGGTGCCCGGAGCCGGAGAAGGTGATCGGACTGACGCCTCATTCCGACTCGGTTGGGCTAACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGAAGGAAATTGGATTACCGTTATGCCCCTTCCAAATGCCTTCATCGTTAACATTGGCGACATCTTGGAGATGGTAACAAATGGGAAATACAAAAGCATAGAGCATTGTGCAACAGTGAACTCAAAGAGTGAAAGGCTGTCCATAGCAACTTTCTACACTCCAAGTTTGGAGAAAGAAATAAGGCCAACACCATCCCTCATCACACCACATTCTCCTCCCTTGTTTCGAACTTTGACATATCAAGAGTATGTGAAGGGCCTTTTCTCTCGAACACTCGACGGAAAAACTTATCTCGACGCTATGCGAATTCACCCTCCTTCAACCTAA

Protein sequence

MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPPST*

Homology

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BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.5e-110
Identity = 196/349 (56.16%), Postives = 249/349 (71.35%), Query Frame = 0


Query: 14  SSILVPSVQELAKH-PITEIPHRYIR--PDLLHHSPIASAASQIPVIDMSNFRSNDTMDS 73
           SSILVPSVQE+ K   IT +P RY+R   D            +IP+IDM    S+ TMDS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 74  ELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQ 133
           E+ +L  ACK WGFFQLV HG+  S ++++K E Q  F LP+EEKKK WQR  ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 134 AFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGI 193
           AF+ SE+QKLDW D+FF    P+  R P LF  LPL  R+TLE YS+ V++V   +I  +
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 194 EKALGIKEGEMSELFKDGN--QSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVE 253
            +AL IK  E+ +LF D +  QSMR+NYYP CP+P++VIGLTPHSDSVGLT+L+Q+N+VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 254 GLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTP 313
           GL+IKK+G W+ V PLPNAFIVNIGD+LE++TNG Y+SIEH   VNS+ ERLSIATF+  
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 314 SLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
            + KE+ P  SL+       F+ LT +EY  GLFSRTLDGK YLDA+RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

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BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.6e-107
Identity = 198/358 (55.31%), Postives = 255/358 (71.23%), Query Frame = 0


Query: 10  LRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAA----SQIPVIDMSNFRSN 69
           ++ G+ + +PSVQELAK  + EIP RYI        P+ ++       +PVID+ N  S+
Sbjct: 7   MKLGNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISS 66

Query: 70  D--TMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREG 129
           +  T   EL RLHSACK WGFFQ+V HGV  SL++ +K + Q  F L + EK K  Q++G
Sbjct: 67  EPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDG 126

Query: 130 DVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVT 189
           DVEGFGQAF+ SE+Q LDW DIF I T PLH R P LF  LPL LRET+E YS+ +K ++
Sbjct: 127 DVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLS 186

Query: 190 TAIIGGIEKAL---GIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 249
             +   +EKAL    ++  E+SE+FKD  Q MR+NYYP CP+PE  IGLTPHSD  GLTI
Sbjct: 187 MVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTI 246

Query: 250 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 309
           LLQ+NEVEGL+IK EG WI+V PLPNAF+VN+GD+LE++TNG Y+S++H A VNS  ERL
Sbjct: 247 LLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERL 306

Query: 310 SIATFYTPSLEKEIRPTPSLITPHSPPLFRT-LTYQEYVKGLFSRTLDGKTYLDAMRI 358
           SIATF+ P+LE EI P  SLITP++P LFR+  TY E V+   SR LDGK++LD+MR+
Sbjct: 307 SIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

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BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.7e-103
Identity = 195/363 (53.72%), Postives = 253/363 (69.70%), Query Frame = 0


Query: 5   KKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPD----LLHHSPIASAASQIPVIDMS 64
           +K   ++ G+ + +PSVQELAK  + EIP RY+  +    L   + + +    IPVID+ 
Sbjct: 2   EKAKLMKLGNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIE 61

Query: 65  NFRSNDTM--DSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKL 124
           N  S + +    EL RLH ACK WGFFQ+V HGV  SL++ +K E Q  F L ++EK K 
Sbjct: 62  NLLSPEPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKY 121

Query: 125 WQREGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAA 184
            Q +GDVEGFGQ FI SE+Q LDW DIF + T PLH R P LF  LP+ LRET+E YS+ 
Sbjct: 122 EQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSE 181

Query: 185 VKNVTTAIIGGIEKALGIKEGE---MSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDS 244
           +K ++  +   +EKAL ++  E   MSE+F DG Q+MR+NYYP CP+P   IGLT HSD 
Sbjct: 182 MKKLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDF 241

Query: 245 VGLTILLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNS 304
            GLTILLQINEVEGL+IK+EG WI+V PLPNAF+VN+GDILE++TNG Y S++H A VNS
Sbjct: 242 GGLTILLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNS 301

Query: 305 KSERLSIATFYTPSLEKEIRPTPSLITPHSPPLFRT-LTYQEYVKGLFSRTLDGKTYLDA 358
            +ERLSIATF+ PSLE  I P  SLITP +P LF++  TY + V+   +R LDGK++LD+
Sbjct: 302 TNERLSIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDS 361

[Close]

BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.0e-100
Identity = 184/354 (51.98%), Postives = 248/354 (70.06%), Query Frame = 0


Query: 10  LRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQ---IPVIDMSNFRSND 69
           ++ G+ + +PSVQELAK  + EIP RY        + I ++ +    +PVID+ N  S +
Sbjct: 7   IKLGNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPE 66

Query: 70  TM--DSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGD 129
            +    EL +LHSACK WGFFQLV HGV   LM+ +K E +  F LP+ EK K  Q++GD
Sbjct: 67  PVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGD 126

Query: 130 VEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTT 189
            EGFGQ +I SE+Q+LDW ++F + + PLH R P LF  LPL  RETLE Y + +K ++T
Sbjct: 127 FEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLST 186

Query: 190 AIIGGIEKALGIKEGE-MSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQ 249
            +   +EK+L + E + M++LF+DG Q+MR+NYYP CP PE V+GLT HSD  GLTILLQ
Sbjct: 187 VVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQ 246

Query: 250 INEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIA 309
           +NEVEGL+I+KE  WI++ PLP+AFIVN+GDILE++TNG Y+S+EH A VNS  ERLSIA
Sbjct: 247 LNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIA 306

Query: 310 TFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
           TF+   LE EI P  SL+TP +P LF+   Y++ +K   SR LDGK++LD MR+
Sbjct: 307 TFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

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BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-76
Identity = 144/348 (41.38%), Postives = 220/348 (63.22%), Query Frame = 0


Query: 13  GSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAAS-QIPVIDMSNFRSNDTMDSE 72
           G S+ V +VQ LA   +  +P+RY+RP+L H   +    S +IPVID+S          E
Sbjct: 9   GGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACDE 68

Query: 73  LSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQA 132
           L++ HSAC +WGFFQL+ HGV + ++E+MK++T+  F+LP +EK    Q    +EG+GQA
Sbjct: 69  LAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYGQA 128

Query: 133 FITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGIE 192
           F+TSEEQKLDW D+ F+ T P+  RN R +   P S RET+E+YS  ++ V   + G + 
Sbjct: 129 FVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCLTGMMA 188

Query: 193 KALGIKEGEMSELFKD--GNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEG 252
           K LG++   +++  +     +   +     C E    +GL+PHSD+ GLT+L+Q+NEV G
Sbjct: 189 KNLGLESEILTKPLRTVFNREDELLPSMSSCGEG---LGLSPHSDATGLTLLIQVNEVNG 248

Query: 253 LKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPS 312
           L IKK+  W+ + P+  AF+VNIGD++E+++NG YKSIEH A +N+  ERLSIA F+ P 
Sbjct: 249 LHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSIAAFHDPE 308

Query: 313 LEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
              +I P P L+  +    ++T+ Y++Y+    +  LDGK+ LD M++
Sbjct: 309 YGTKIGPLPDLVKENGVK-YKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

[Close]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL
Match: A0A1S3CK11 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=LOC103501850 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.9e-187
Identity = 325/362 (89.78%), Postives = 343/362 (94.75%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEK   TLR+GSSILVPSVQELAKHPITEIPHRYI PDLLHHSPI+S ASQIPVIDMS
Sbjct: 1   MAEEKN-DTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           N RS+DTMDSELSRLHSACKNWGFFQLVKHGVSDSL+ERMKMETQKLF+LPIEEKKKLWQ
Sbjct: 61  NLRSHDTMDSELSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAF+TSEEQKLDWCDIFFIAT PLHFR PRLFQNLPLSLRETLEEYSAAVK
Sbjct: 121 REGDVEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +V   I+ G+EKALGIKEGE+SELFKDGNQSMR+NYYP CPEPEKVIGLTPHSD+ GLTI
Sbjct: 181 DVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300
           LLQIN+VEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNG+YKSIEHCATVNSKSERL
Sbjct: 241 LLQINKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERL 300

Query: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360
           SIATF +PSLEKEIRPTPSLITPHSPPLF TLTYQEYV+G+FSR LDGK+YLDAMRIH  
Sbjct: 301 SIATFNSPSLEKEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIHHL 360

Query: 361 ST 363
           ST
Sbjct: 361 ST 361

[Close]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL
Match: A0A6J1F704 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.4e-156
Identity = 273/359 (76.04%), Postives = 309/359 (86.07%), Query Frame = 0


Query: 5   KKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFRS 64
           +K +T RFG SILVPSVQELAK PI E+P RYIRPD LH  PI SAAS+IPVIDMSNF S
Sbjct: 3   EKNNTSRFGGSILVPSVQELAKQPIIEVPSRYIRPD-LHSFPIVSAASEIPVIDMSNFHS 62

Query: 65  NDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKK-LWQREG 124
           + +M SEL+RLHSACKNWGFFQLV HGVSDSL+E+MK ETQ  F LPIEEKKK LWQ E 
Sbjct: 63  DASMHSELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQMFFNLPIEEKKKRLWQSEE 122

Query: 125 DVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVT 184
           D EGFGQAF+ SEEQKLDWCD+FFIAT+P H R PRLFQNLPL+ R T+EEYSA VKN+ 
Sbjct: 123 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 182

Query: 185 TAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQ 244
            AI    EKALGIKEGE++E+FKDG+QSMR+NYYP CPEPEKVIGLTPHSD+VGLTILLQ
Sbjct: 183 MAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 242

Query: 245 INEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIA 304
           INE+EGLKIKK+GNW+ V PLPNAFIVNIGDI+E++TNG+YKSIEHCATVN+K ERLSIA
Sbjct: 243 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIA 302

Query: 305 TFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPPST 363
           TF  P LEKEI+  PSLITPHSPPLF TLTYQ++V+ LFSR LDGK++LD +RIH P T
Sbjct: 303 TFNNPRLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTPPT 360

[Close]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL
Match: A0A6J1BUE5 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 260/351 (74.07%), Postives = 299/351 (85.19%), Query Frame = 0


Query: 12  FGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFRSNDTMDSE 71
           +GSSI VPSVQELAK P  EIP RY+R DLL  SPI SAAS+IPVID ++  S  +MDSE
Sbjct: 50  YGSSIPVPSVQELAKQPFIEIPPRYVRSDLL-RSPIVSAASEIPVIDFASLLSGASMDSE 109

Query: 72  LSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQA 131
            +RLHSAC++WGFFQLV HGV    +E+M++ETQ+ F+LPIEE+KKLWQ EGDVEGFGQA
Sbjct: 110 SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEERKKLWQIEGDVEGFGQA 169

Query: 132 FITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGIE 191
           F+ SEEQKLDW DIFF+ T+P H R PRLFQNLPLS R  LE+YS+ VK+V  AI+  +E
Sbjct: 170 FVVSEEQKLDWSDIFFLCTAPEHQRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 229

Query: 192 KALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEGLK 251
           +AL IKEGEM+E+FKDGNQSMR+NYYP CPE EKVIGLTPHSD+VGLTILLQIN+VEGLK
Sbjct: 230 RALEIKEGEMTEMFKDGNQSMRMNYYPACPETEKVIGLTPHSDAVGLTILLQINQVEGLK 289

Query: 252 IKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPSLE 311
           IKKEG WI V PLP+AFIVNIGDI+EM+TNGKY+SIEHCATVNS+SERLSIATF  PS E
Sbjct: 290 IKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFE 349

Query: 312 KEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI-HPPS 362
           K IRP PSLITPHSPPLF +LTYQ YV GLF+R LDGK+YLDAMRI HPP+
Sbjct: 350 KLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHPPT 396

[Close]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL
Match: A0A5D3DVK8 (Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00240 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.3e-141
Identity = 250/279 (89.61%), Postives = 265/279 (94.98%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEK   TLR+GSSILVPSVQELAKHPITEIPHRYI PDLLHHSPI+S ASQIPVIDMS
Sbjct: 1   MAEEKN-DTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           N RS+DTMDSELSRLHSACKNWGFFQLVKHGVSDSL+ERMKMETQKLF+LPIEEKKKLWQ
Sbjct: 61  NLRSHDTMDSELSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAF+TSEEQKLDWCDIFFIAT PLHFR PRLFQNLPLSLRETLEEYSAAVK
Sbjct: 121 REGDVEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +V   I+ G+EKALGIKEGE+SELFKDGNQSMR+NYYP CPEPEKVIGLTPHSD+ GLTI
Sbjct: 181 DVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMV 280
           LLQIN+VEGLKIKKEGNWITVMPLPNAFIVNIGDILE++
Sbjct: 241 LLQINKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEVI 278

[Close]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL
Match: A0A5D3DWN6 (Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00250 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.8e-141
Identity = 248/279 (88.89%), Postives = 263/279 (94.27%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEK   TLR+GSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPI+S ASQIPVIDMS
Sbjct: 1   MAEEKNNDTLRYGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPISSDASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           N RS+DTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLF+LPIEEKKKLWQ
Sbjct: 61  NLRSHDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFELPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           R GDVEGFGQAF+ SEEQKLDWCD+FF+AT PLHFRNPRL QNLPLSLRETLEEYSAAVK
Sbjct: 121 RAGDVEGFGQAFVRSEEQKLDWCDMFFMATLPLHFRNPRLLQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +V T I  G+EKALGIKEGE+SELFK+GNQSMR+NYYP CPEPEKVIGL PHSDSVGLTI
Sbjct: 181 DVATEIFDGVEKALGIKEGELSELFKNGNQSMRMNYYPPCPEPEKVIGLAPHSDSVGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMV 280
           LLQIN+VEGLKIKKEGNWI VMPLPNAFIVNIGDILE++
Sbjct: 241 LLQINKVEGLKIKKEGNWINVMPLPNAFIVNIGDILEVI 279

[Close]

BLAST of CSPI04G22430 vs. NCBI nr
Match: XP_004146011.3 (LOW QUALITY PROTEIN: protein SRG1 [Cucumis sativus])

HSP 1 Score: 730.7 bits (1885), Expect = 6.1e-207
Identity = 360/362 (99.45%), Postives = 361/362 (99.72%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS
Sbjct: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ
Sbjct: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAFITSEEQKLDWCDIFFIATSP HFRNPRLFQNLPLSLRETLEEYSAAVK
Sbjct: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPSHFRNPRLFQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +VTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI
Sbjct: 181 DVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300
           LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL
Sbjct: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300

Query: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360
           SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP
Sbjct: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360

Query: 361 ST 363
           ST
Sbjct: 361 ST 362

[Close]

BLAST of CSPI04G22430 vs. NCBI nr
Match: KGN55530.2 (hypothetical protein Csa_012011 [Cucumis sativus])

HSP 1 Score: 730.7 bits (1885), Expect = 6.1e-207
Identity = 360/362 (99.45%), Postives = 361/362 (99.72%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS
Sbjct: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ
Sbjct: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAFITSEEQKLDWCDIFFIATSP HFRNPRLFQNLPLSLRETLEEYSAAVK
Sbjct: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPSHFRNPRLFQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +VTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI
Sbjct: 181 DVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300
           LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL
Sbjct: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300

Query: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360
           SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP
Sbjct: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360

Query: 361 ST 363
           ST
Sbjct: 361 ST 362

[Close]

BLAST of CSPI04G22430 vs. NCBI nr
Match: XP_008463796.1 (PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo])

HSP 1 Score: 662.5 bits (1708), Expect = 2.0e-186
Identity = 325/362 (89.78%), Postives = 343/362 (94.75%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEEK   TLR+GSSILVPSVQELAKHPITEIPHRYI PDLLHHSPI+S ASQIPVIDMS
Sbjct: 1   MAEEKN-DTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           N RS+DTMDSELSRLHSACKNWGFFQLVKHGVSDSL+ERMKMETQKLF+LPIEEKKKLWQ
Sbjct: 61  NLRSHDTMDSELSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAF+TSEEQKLDWCDIFFIAT PLHFR PRLFQNLPLSLRETLEEYSAAVK
Sbjct: 121 REGDVEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           +V   I+ G+EKALGIKEGE+SELFKDGNQSMR+NYYP CPEPEKVIGLTPHSD+ GLTI
Sbjct: 181 DVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300
           LLQIN+VEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNG+YKSIEHCATVNSKSERL
Sbjct: 241 LLQINKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERL 300

Query: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360
           SIATF +PSLEKEIRPTPSLITPHSPPLF TLTYQEYV+G+FSR LDGK+YLDAMRIH  
Sbjct: 301 SIATFNSPSLEKEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIHHL 360

Query: 361 ST 363
           ST
Sbjct: 361 ST 361

[Close]

BLAST of CSPI04G22430 vs. NCBI nr
Match: XP_038897143.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 293/358 (81.84%), Postives = 327/358 (91.34%), Query Frame = 0


Query: 1   MAEEKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMS 60
           MAEE   +T R+GSSILVPSVQELAK PITEIP RYIRPDLLHH PIASAAS+IPVIDMS
Sbjct: 1   MAEENSTNTSRYGSSILVPSVQELAKQPITEIPPRYIRPDLLHHVPIASAASEIPVIDMS 60

Query: 61  NFRSNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQ 120
           NF S+ T+DSEL+RLHSACKNWGFFQLV HGVSDSL+E+MK+ET+KLF LPIEEKKKLWQ
Sbjct: 61  NFHSDATIDSELARLHSACKNWGFFQLVNHGVSDSLIEKMKIETKKLFDLPIEEKKKLWQ 120

Query: 121 REGDVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVK 180
           REGDVEGFGQAF+ SEEQKLDW D+FFIATSP H R  RLFQNLPL+ R+TLE+YSA VK
Sbjct: 121 REGDVEGFGQAFVVSEEQKLDWSDLFFIATSPSHLRKQRLFQNLPLTFRKTLEDYSAEVK 180

Query: 181 NVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTI 240
           ++  AI  G+EK LGIK+GE+ E+FKDGNQSMR+NYYPRCPEPEK IGLTPHSD+VGLTI
Sbjct: 181 DLAAAIFEGVEKVLGIKKGELKEMFKDGNQSMRMNYYPRCPEPEKAIGLTPHSDAVGLTI 240

Query: 241 LLQINEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERL 300
           LLQINEVEGLKIKK+GNWI VMPLPNAF+VN+GDI+EMVTNG+YKSIEHCATVNSKSERL
Sbjct: 241 LLQINEVEGLKIKKDGNWIPVMPLPNAFVVNVGDIMEMVTNGEYKSIEHCATVNSKSERL 300

Query: 301 SIATFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIH 359
           SIATF  PS+EKEIRP PSLITPHSPPLF+TLTYQE+VKGLF++ LDGK+YLDAMRIH
Sbjct: 301 SIATFNNPSMEKEIRPIPSLITPHSPPLFKTLTYQEFVKGLFTQKLDGKSYLDAMRIH 358

[Close]

BLAST of CSPI04G22430 vs. NCBI nr
Match: XP_023534939.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.0 bits (1460), Expect = 1.2e-157
Identity = 274/356 (76.97%), Postives = 307/356 (86.24%), Query Frame = 0


Query: 4   EKKIHTLRFGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFR 63
           EK  +T RFG SILVPSVQELAK PI E+P RYIRPD LH  PI SAAS+IPVIDMSNF 
Sbjct: 3   EKNNNTSRFGGSILVPSVQELAKQPIVEVPSRYIRPD-LHSFPIVSAASEIPVIDMSNFH 62

Query: 64  SNDTMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREG 123
           SN +M SEL+RLHSACKNWGFFQLV HGVSD LME+MK ETQ  F LPIEEKKKLWQ E 
Sbjct: 63  SNASMYSELARLHSACKNWGFFQLVNHGVSDLLMEKMKAETQMFFNLPIEEKKKLWQSEE 122

Query: 124 DVEGFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVT 183
           D EGFGQAF+ SEEQKLDWCD+FFIAT+P H R PRLFQNLPL+ R T+EEYSA VKN+ 
Sbjct: 123 DAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLA 182

Query: 184 TAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQ 243
            AI    EKALGIK+GE++E+FKDG+QSMR+NYYP CPEPEKVIGLTPHSD+VGLTILLQ
Sbjct: 183 IAIFEQFEKALGIKDGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQ 242

Query: 244 INEVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIA 303
           INE+EGLKIKK+GNW+ V PLPNAFIVNIGDI+E+ TNG+YKSIEHCATVN+K ERLSIA
Sbjct: 243 INEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEITTNGEYKSIEHCATVNAKRERLSIA 302

Query: 304 TFYTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHP 360
           TF  PSLEKEIRP PSL+TPHSPPLF TLTYQ++V+ LFSR LDGK++LD +RI P
Sbjct: 303 TFNNPSLEKEIRPIPSLLTPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIQP 357

[Close]

BLAST of CSPI04G22430 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 7.9e-112
Identity = 196/348 (56.32%), Postives = 248/348 (71.26%), Query Frame = 0


Query: 14  SSILVPSVQELAKHP-ITEIPHRYIR--PDLLHHSPIASAASQIPVIDMSNFRSNDTMDS 73
           SSI+VPSVQE+ K   IT +P RY+R   D+   +  +   +QIP+IDMS   S+ +MDS
Sbjct: 9   SSIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDS 68

Query: 74  ELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQ 133
           E+ +L SACK WGFFQLV HG+  S + ++K E Q  F LP+EEKK LWQ+  ++EGFGQ
Sbjct: 69  EIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQ 128

Query: 134 AFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGI 193
            F+ SEEQKLDW D+FF+   P+  R P LF  LPL  R+TL+ YSA VK++   ++G I
Sbjct: 129 VFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKI 188

Query: 194 EKALGIKEGEMSELFKDG-NQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEG 253
             AL IK  EM +LF D   Q +R+NYYPRCPEP+KVIGLTPHSDS GLTILLQ NEVEG
Sbjct: 189 AVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVEG 248

Query: 254 LKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPS 313
           L+IKK   W++V PLPNA +VN+GDILE++TNG Y+SIEH   VNS+ ERLS+A F+   
Sbjct: 249 LQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIG 308

Query: 314 LEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
           L KEI P  SL+  H    F+++T +EY  GLFSR LDGK YLD MR+
Sbjct: 309 LGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

[Close]

BLAST of CSPI04G22430 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-111
Identity = 196/349 (56.16%), Postives = 249/349 (71.35%), Query Frame = 0


Query: 14  SSILVPSVQELAKH-PITEIPHRYIR--PDLLHHSPIASAASQIPVIDMSNFRSNDTMDS 73
           SSILVPSVQE+ K   IT +P RY+R   D            +IP+IDM    S+ TMDS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 74  ELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQ 133
           E+ +L  ACK WGFFQLV HG+  S ++++K E Q  F LP+EEKKK WQR  ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 134 AFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGI 193
           AF+ SE+QKLDW D+FF    P+  R P LF  LPL  R+TLE YS+ V++V   +I  +
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 194 EKALGIKEGEMSELFKDGN--QSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVE 253
            +AL IK  E+ +LF D +  QSMR+NYYP CP+P++VIGLTPHSDSVGLT+L+Q+N+VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 254 GLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTP 313
           GL+IKK+G W+ V PLPNAFIVNIGD+LE++TNG Y+SIEH   VNS+ ERLSIATF+  
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 314 SLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
            + KE+ P  SL+       F+ LT +EY  GLFSRTLDGK YLDA+RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of CSPI04G22430 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-111
Identity = 195/348 (56.03%), Postives = 249/348 (71.55%), Query Frame = 0


Query: 14  SSILVPSVQELAKHPI--TEIPHRYIRPDL-LHHSPIASAASQIPVIDMSNFRSNDTMDS 73
           SS++VPSVQE+ K  +  T +P RY+R D     + I S  +QIP+IDMS   S+ +MDS
Sbjct: 9   SSVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDS 68

Query: 74  ELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQ 133
           E+ +L  ACK WGFFQLV HG+    +++ K + Q  F LP+EEKKKLWQ+ GD+EGFGQ
Sbjct: 69  EIDKLDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQ 128

Query: 134 AFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAIIGGI 193
           AF+ SEEQKLDW D+FF+   P+  R P LF  LPL  R+TL+ YSA +K++   +   +
Sbjct: 129 AFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKL 188

Query: 194 EKALGIKEGEMSELFKDG-NQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEG 253
             AL IK  EM +LF D   Q +R+NYYP CPEP+K IGLTPHSD+ GLTILLQ+NEVEG
Sbjct: 189 ASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEG 248

Query: 254 LKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPS 313
           L+IKK+G W++V PLPNA +VN+GDILE++TNG Y+SIEH   VNS+ ERLS+A+F+   
Sbjct: 249 LQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTG 308

Query: 314 LEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
             KEI P  SL+  H   LF+TLT +EY  GLFSR LDGK YLD MRI
Sbjct: 309 FGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

[Close]

BLAST of CSPI04G22430 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.6e-104
Identity = 190/352 (53.98%), Postives = 245/352 (69.60%), Query Frame = 0


Query: 14  SSILVPSVQELAKHP-ITEIPHRYIRPD------LLHHSPIASAASQIPVIDMSNFRSND 73
           SSILVPSVQE+ K   IT +P RY+R D      ++H S +    S+IP+IDM+   S+ 
Sbjct: 10  SSILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGL---ISEIPIIDMNRLCSST 69

Query: 74  TMDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVE 133
            +DSE+ +L  ACK +GFFQLV HG+  S ++++K E Q  F LP+EEKKKLWQ    +E
Sbjct: 70  AVDSEVEKLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVME 129

Query: 134 GFGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAI 193
           GFGQAF+ SE+QKLDW D+FF+   P+  R   LF  LPL  R+TL+ YS  VK++   +
Sbjct: 130 GFGQAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKIL 189

Query: 194 IGGIEKALGIKEGEMSELFKDG-NQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQIN 253
           +  + KAL IK  E+ E+F D   QSMR+NYYP CP+P  V GL PHSD+VGLTILLQ+N
Sbjct: 190 LAKMAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVN 249

Query: 254 EVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATF 313
           EV+GL+IKK G W  V PL NAFIVN+GD+LE++TNG Y+SIEH A VN + ERLSIATF
Sbjct: 250 EVDGLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATF 309

Query: 314 YTPSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
           +   ++KEI P  SL+       FR+L  ++Y+ GLFSR L GK YLDAMRI
Sbjct: 310 HNTGMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRI 358

[Close]

BLAST of CSPI04G22430 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.1e-100
Identity = 179/350 (51.14%), Postives = 244/350 (69.71%), Query Frame = 0


Query: 14  SSILVPSVQELAKHP-ITEIPHRYIRPD-----LLHHSPIASAASQIPVIDMSNFRSNDT 73
           SS++VP V E+ K    T IP RY+R D     +L+ S   S +S+IPVIDM+   S   
Sbjct: 10  SSLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDS---SLSSEIPVIDMTRLCSVSA 69

Query: 74  MDSELSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEG 133
           MDSEL +L  AC++WGFFQLV HG+  S +E+++ E Q+ F LP++EK+KLWQR G+ EG
Sbjct: 70  MDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEG 129

Query: 134 FGQAFITSEEQKLDWCDIFFIATSPLHFRNPRLFQNLPLSLRETLEEYSAAVKNVTTAII 193
           FGQ  I SE QKLDW D+F + T P+  R   LF  LP   RETLE YS+ VK++   + 
Sbjct: 130 FGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILF 189

Query: 194 GGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEV 253
             +   L IK  EM +LF D  QS++INYYP CP+P++V+GLT HSD+ GLTILLQ+N+V
Sbjct: 190 AKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQV 249

Query: 254 EGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYT 313
           EGL+IKK+G W+ V PL +A +VN+G+ILE++TNG+Y+SIEH A VNS+ ERLS+A F++
Sbjct: 250 EGLQIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHS 309

Query: 314 PSLEKEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRI 358
           P  E  IRP  SL+      LF++++ QEY    F++ L+GK++LD MRI
Sbjct: 310 PGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

The following BLAST results are available for this feature:

BLAST of CSPI04G22430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39224	5.5e-110	56.16	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N501	2.6e-107	55.31	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
D4N500	1.7e-103	53.72	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
D4N502	3.0e-100	51.98	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
A2A1A0	1.1e-76	41.38	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]

BLAST of CSPI04G22430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CK11	9.9e-187	89.78	probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1F704	2.4e-156	76.04	protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1	[more]
A0A6J1BUE5	1.3e-146	74.07	protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1	[more]
A0A5D3DVK8	1.3e-141	89.61	Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa...	[more]
A0A5D3DWN6	4.8e-141	88.89	Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa...	[more]

BLAST of CSPI04G22430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146011.3	6.1e-207	99.45	LOW QUALITY PROTEIN: protein SRG1 [Cucumis sativus]	[more]
KGN55530.2	6.1e-207	99.45	hypothetical protein Csa_012011 [Cucumis sativus]	[more]
XP_008463796.1	2.0e-186	89.78	PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo]	[more]
XP_038897143.1	1.1e-171	81.84	protein SRG1-like [Benincasa hispida]	[more]
XP_023534939.1	1.2e-157	76.97	protein SRG1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI04G22430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25300.1	7.9e-112	56.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	3.9e-111	56.16	senescence-related gene 1	[more]
AT4G25310.1	3.9e-111	56.03	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17010.1	3.6e-104	53.98	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78550.1	1.1e-100	51.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 209..308 e-value: 3.7E-26 score: 91.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 16..346 e-value: 8.8E-102 score: 342.8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 54..152 e-value: 4.5E-27 score: 95.1
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 13..357
None	No IPR available	PANTHER	PTHR47991:SF126	2OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASE	coord: 13..357
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 18..349
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 208..308 score: 13.563128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G22430.1	CSPI04G22430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding