CSPI04G22420 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G22420
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr4: 20716421 .. 20717430 (-)
RNA-Seq Expression	CSPI04G22420
Synteny	CSPI04G22420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAGGAGAAGAAGATCGACACGTTAAGGTTAGGAAGTTCCATTCTAGTTCCTTCCGTTCAAGAGTTACCCAAACACCCCATCACCGAAATTCCACATCGATACACCCGCCCCGATCTCCTCCACCACTCCCCGATCACCTCCGCTTCCCAAATCCCCGTGATCGACATGTCCAACTTCCGCTCCGATCATACAATGGACTCCGAATTGTCTCGCCTTCACTCCGCCTGCAAAAACTGGGGATTCTTTTAGGTTCATAATATCTCCTTTCTCTATCTCTGTTTCTATGTGTTTAATTAGTAAGTAGAATCTGAAAATGATCGGTGAAATTAATTACAGTTGGTGAAGCATGGAGTGAGCGATTCGCTGATGGAGATAATGAAAATGGAAACGCAGAAATTGTTCCAACTTCCGATTGAAGAGAAGGAAAAGCTATGGCAGATAGAAGGAGATGTAGAAGGATTTGGACAAGCTTTCGTTAGAAGTAAAGAACAGAAATTGGATTGGTGCGATATTTTCTTCATCGCTACATCGCCTTTACATTTCAGGAAACCCCGGTTATTTCAAAATCTTCCTCTCTCTTTGAGGTTGGATAATTCCTTCCATTGCAAAACTTTTAAAGTGATTCTCAAACTCATAAAACTCCTAGAAATTGAACTCTGGACTCATAAACAAATGTTGATTATGAAACTGTATTGGTATGCAGAGAGACATTGGAAGAGTACTCGGCGGCGGTGAAGGATGTGACGGCGGCGATATTTGGTGGAGTTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGTTGAGTGAGTTGTTCAAGGAGGGTAATCAATCGATGAGAATGAACTATTACCCACCCTGCCCGGAGCCAGAGAAGGTGATCGGACTGACGCCTCATTCCGACCCCGTTGGACTCACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGACGGGAATTCGATTACCGTTATACCCCTCCCAAATGCTTCATAG

mRNA sequence

ATGGCGGAGGAGAAGAAGATCGACACGTTAAGAGAGACATTGGAAGAGTACTCGGCGGCGGTGAAGGATGTGACGGCGGCGATATTTGGTGGAGTTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGTTGAGTGAGTTGTTCAAGGAGGGTAATCAATCGATGAGAATGAACTATTACCCACCCTGCCCGGAGCCAGAGAAGGTGATCGGACTGACGCCTCATTCCGACCCCGTTGGACTCACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGACGGGAATTCGATTACCGTTATACCCCTCCCAAATGCTTCATAG

Coding sequence (CDS)

ATGGCGGAGGAGAAGAAGATCGACACGTTAAGAGAGACATTGGAAGAGTACTCGGCGGCGGTGAAGGATGTGACGGCGGCGATATTTGGTGGAGTTGAGAAAGCGTTGGGGATAAAAGAGGGAGAGTTGAGTGAGTTGTTCAAGGAGGGTAATCAATCGATGAGAATGAACTATTACCCACCCTGCCCGGAGCCAGAGAAGGTGATCGGACTGACGCCTCATTCCGACCCCGTTGGACTCACGATTCTGCTCCAAATCAACGAAGTAGAAGGTCTGAAGATTAAGAAAGACGGGAATTCGATTACCGTTATACCCCTCCCAAATGCTTCATAG

Protein sequence

MAEEKKIDTLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNAS*

Homology

[Close]

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 8.0e-28
Identity = 62/103 (60.19%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 10  LRETLEEYSAAVKDVTAAIFGGVEKAL---GIKEGELSELFKEGNQSMRMNYYPPCPEPE 69
           LRET+E YS+ +K ++  +F  +EKAL    ++  E+SE+FK+  Q MRMNYYPPCP+PE
Sbjct: 171 LRETIESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPE 230

Query: 70  KVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
             IGLTPHSD  GLTILLQ+NEVEGL+IK +G  I+V PLPNA
Sbjct: 231 LAIGLTPHSDFGGLTILLQLNEVEGLQIKNEGRWISVKPLPNA 273

[Close]

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 8.0e-28
Identity = 60/101 (59.41%), Postives = 78/101 (77.23%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGN--QSMRMNYYPPCPEPEKV 70
           R+TLE YS+ V+ V   +   + +AL IK  EL +LF + +  QSMRMNYYPPCP+P++V
Sbjct: 168 RDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQV 227

Query: 71  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD VGLT+L+Q+N+VEGL+IKKDG  + V PLPNA
Sbjct: 228 IGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

[Close]

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-27
Identity = 61/103 (59.22%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 10  LRETLEEYSAAVKDVTAAIFGGVEKALGIKEGE---LSELFKEGNQSMRMNYYPPCPEPE 69
           LRET+E YS+ +K ++  +F  +EKAL ++  E   +SE+F +G Q+MRMNYYPPCP+P 
Sbjct: 171 LRETIESYSSEMKKLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPN 230

Query: 70  KVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
             IGLT HSD  GLTILLQINEVEGL+IK++G  I+V PLPNA
Sbjct: 231 LAIGLTSHSDFGGLTILLQINEVEGLQIKREGTWISVKPLPNA 273

[Close]

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.9e-24
Identity = 55/100 (55.00%), Postives = 78/100 (78.00%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGE-LSELFKEGNQSMRMNYYPPCPEPEKVI 70
           RETLE Y + +K ++  +F  +EK+L + E + +++LF++G Q+MRMNYYPPCP PE V+
Sbjct: 171 RETLESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVL 230

Query: 71  GLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           GLT HSD  GLTILLQ+NEVEGL+I+K+   I++ PLP+A
Sbjct: 231 GLTSHSDFSGLTILLQLNEVEGLQIRKEERWISIKPLPDA 270

[Close]

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.4e-19
Identity = 46/102 (45.10%), Postives = 70/102 (68.63%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           + R++++ YS+  K +   +F  + KA+G K   L +LF+E  + +RM YYPPC + +KV
Sbjct: 166 SFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEEQPRGLRMAYYPPCRQADKV 225

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNAS 111
           +GL+PHSD  GLT+LL+IN V+GL+IKKDG   + I  PN +
Sbjct: 226 MGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFS-IDAPNGA 266

[Close]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL
Match: A0A0A0L372 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G622810 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.0e-54
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0


Query: 1   MAEEKKIDTLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYP 60
           MAEEKKIDTLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYP
Sbjct: 1   MAEEKKIDTLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYP 60

Query: 61  PCPEPEKVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNAS 111
           PCPEPEKVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNAS
Sbjct: 61  PCPEPEKVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNAS 110

[Close]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL
Match: A0A0A0KZZ7 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G622820 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.0e-47
Identity = 97/109 (88.99%), Postives = 103/109 (94.50%), Query Frame = 0


Query: 1   MAEEKKIDTLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYP 60
           MAEEKKI TLRETLEEYSAAVKDVT AI GG+EKALGIKEGE+SELFK+GNQSMR+NYYP
Sbjct: 1   MAEEKKIHTLRETLEEYSAAVKDVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYP 60

Query: 61  PCPEPEKVIGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
            CPEPEKVIGLTPHSD VGLTILLQINEVEGLKIKK+GN ITV+PLPNA
Sbjct: 61  RCPEPEKVIGLTPHSDSVGLTILLQINEVEGLKIKKEGNWITVMPLPNA 109

[Close]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL
Match: A0A5D3DVK8 (Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00240 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.0e-42
Identity = 89/101 (88.12%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV A I  GVEKALGIKEGELSELFK+GNQSMRMNYYPPCPEPEKV
Sbjct: 166 SLRETLEEYSAAVKDVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKV 225

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD  GLTILLQIN+VEGLKIKK+GN ITV+PLPNA
Sbjct: 226 IGLTPHSDAAGLTILLQINKVEGLKIKKEGNWITVMPLPNA 266

[Close]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL
Match: A0A1S3CK11 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=LOC103501850 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.0e-42
Identity = 89/101 (88.12%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV A I  GVEKALGIKEGELSELFK+GNQSMRMNYYPPCPEPEKV
Sbjct: 166 SLRETLEEYSAAVKDVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKV 225

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD  GLTILLQIN+VEGLKIKK+GN ITV+PLPNA
Sbjct: 226 IGLTPHSDAAGLTILLQINKVEGLKIKKEGNWITVMPLPNA 266

[Close]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL
Match: A0A5D3DWN6 (Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00250 PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.0e-41
Identity = 88/101 (87.13%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV   IF GVEKALGIKEGELSELFK GNQSMRMNYYPPCPEPEKV
Sbjct: 167 SLRETLEEYSAAVKDVATEIFDGVEKALGIKEGELSELFKNGNQSMRMNYYPPCPEPEKV 226

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGL PHSD VGLTILLQIN+VEGLKIKK+GN I V+PLPNA
Sbjct: 227 IGLAPHSDSVGLTILLQINKVEGLKIKKEGNWINVMPLPNA 267

[Close]

BLAST of CSPI04G22420 vs. NCBI nr
Match: XP_004146011.3 (LOW QUALITY PROTEIN: protein SRG1 [Cucumis sativus])

HSP 1 Score: 201.4 bits (511), Expect = 3.9e-48
Identity = 99/100 (99.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV
Sbjct: 554 SLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 613

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPN 109
           IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPN
Sbjct: 614 IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPN 653

[Close]

BLAST of CSPI04G22420 vs. NCBI nr
Match: KGN55530.2 (hypothetical protein Csa_012011 [Cucumis sativus])

HSP 1 Score: 183.3 bits (464), Expect = 1.1e-42
Identity = 89/101 (88.12%), Postives = 96/101 (95.05%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDVT AI GG+EKALGIKEGE+SELFK+GNQSMR+NYYP CPEPEKV
Sbjct: 167 SLRETLEEYSAAVKDVTTAIIGGIEKALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKV 226

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD VGLTILLQINEVEGLKIKK+GN ITV+PLPNA
Sbjct: 227 IGLTPHSDSVGLTILLQINEVEGLKIKKEGNWITVMPLPNA 267

[Close]

BLAST of CSPI04G22420 vs. NCBI nr
Match: KAA0066697.1 (putative 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo var. makuwa] >TYK27847.1 putative 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 181.4 bits (459), Expect = 4.2e-42
Identity = 89/101 (88.12%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV A I  GVEKALGIKEGELSELFK+GNQSMRMNYYPPCPEPEKV
Sbjct: 166 SLRETLEEYSAAVKDVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKV 225

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD  GLTILLQIN+VEGLKIKK+GN ITV+PLPNA
Sbjct: 226 IGLTPHSDAAGLTILLQINKVEGLKIKKEGNWITVMPLPNA 266

[Close]

BLAST of CSPI04G22420 vs. NCBI nr
Match: XP_008463796.1 (PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo])

HSP 1 Score: 181.4 bits (459), Expect = 4.2e-42
Identity = 89/101 (88.12%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV A I  GVEKALGIKEGELSELFK+GNQSMRMNYYPPCPEPEKV
Sbjct: 166 SLRETLEEYSAAVKDVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKV 225

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD  GLTILLQIN+VEGLKIKK+GN ITV+PLPNA
Sbjct: 226 IGLTPHSDAAGLTILLQINKVEGLKIKKEGNWITVMPLPNA 266

[Close]

BLAST of CSPI04G22420 vs. NCBI nr
Match: KAA0066698.1 (putative 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 179.1 bits (453), Expect = 2.1e-41
Identity = 88/101 (87.13%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 9   TLRETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKV 68
           +LRETLEEYSAAVKDV   IF GVEKALGIKEGELSELFK GNQSMRMNYYPPCPEPEKV
Sbjct: 167 SLRETLEEYSAAVKDVATEIFDGVEKALGIKEGELSELFKNGNQSMRMNYYPPCPEPEKV 226

Query: 69  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGL PHSD VGLTILLQIN+VEGLKIKK+GN I V+PLPNA
Sbjct: 227 IGLAPHSDSVGLTILLQINKVEGLKIKKEGNWINVMPLPNA 267

[Close]

BLAST of CSPI04G22420 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 125.9 bits (315), Expect = 2.0e-29
Identity = 61/100 (61.00%), Postives = 75/100 (75.00%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELF-KEGNQSMRMNYYPPCPEPEKVI 70
           R+TL+ YSA +K +   +F  +  AL IK  E+ +LF  E  Q +RMNYYPPCPEP+K I
Sbjct: 164 RDTLDTYSAELKSIAKVLFAKLASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAI 223

Query: 71  GLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           GLTPHSD  GLTILLQ+NEVEGL+IKKDG  ++V PLPNA
Sbjct: 224 GLTPHSDATGLTILLQVNEVEGLQIKKDGKWVSVKPLPNA 263

[Close]

BLAST of CSPI04G22420 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 124.4 bits (311), Expect = 5.7e-29
Identity = 60/101 (59.41%), Postives = 78/101 (77.23%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGN--QSMRMNYYPPCPEPEKV 70
           R+TLE YS+ V+ V   +   + +AL IK  EL +LF + +  QSMRMNYYPPCP+P++V
Sbjct: 168 RDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQV 227

Query: 71  IGLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           IGLTPHSD VGLT+L+Q+N+VEGL+IKKDG  + V PLPNA
Sbjct: 228 IGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

[Close]

BLAST of CSPI04G22420 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 7.0e-27
Identity = 59/100 (59.00%), Postives = 73/100 (73.00%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELF-KEGNQSMRMNYYPPCPEPEKVI 70
           R+TL+ YSA VK +   + G +  AL IK  E+ +LF  E  Q +R+NYYP CPEP+KVI
Sbjct: 167 RDTLDMYSAEVKSIAKILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVI 226

Query: 71  GLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           GLTPHSD  GLTILLQ NEVEGL+IKK+   ++V PLPNA
Sbjct: 227 GLTPHSDSTGLTILLQANEVEGLQIKKNAKWVSVKPLPNA 266

[Close]

BLAST of CSPI04G22420 vs. TAIR 10
Match: AT4G25300.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 7.0e-27
Identity = 59/100 (59.00%), Postives = 73/100 (73.00%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELF-KEGNQSMRMNYYPPCPEPEKVI 70
           R+TL+ YSA VK +   + G +  AL IK  E+ +LF  E  Q +R+NYYP CPEP+KVI
Sbjct: 73  RDTLDMYSAEVKSIAKILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVI 132

Query: 71  GLTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           GLTPHSD  GLTILLQ NEVEGL+IKK+   ++V PLPNA
Sbjct: 133 GLTPHSDSTGLTILLQANEVEGLQIKKNAKWVSVKPLPNA 172

[Close]

BLAST of CSPI04G22420 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 1.6e-26
Identity = 54/99 (54.55%), Postives = 73/99 (73.74%), Query Frame = 0


Query: 11  RETLEEYSAAVKDVTAAIFGGVEKALGIKEGELSELFKEGNQSMRMNYYPPCPEPEKVIG 70
           RETLE YS+ VK +   +F  +   L IK  E+ +LF +  QS+++NYYPPCP+P++V+G
Sbjct: 168 RETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMG 227

Query: 71  LTPHSDPVGLTILLQINEVEGLKIKKDGNSITVIPLPNA 110
           LT HSD  GLTILLQ+N+VEGL+IKKDG  + V PL +A
Sbjct: 228 LTQHSDAAGLTILLQVNQVEGLQIKKDGKWVVVKPLRDA 266

The following BLAST results are available for this feature:

BLAST of CSPI04G22420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D4N501	8.0e-28	60.19	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
Q39224	8.0e-28	59.41	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N500	1.0e-27	59.22	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
D4N502	1.9e-24	55.00	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
Q94LP4	1.4e-19	45.10	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]

BLAST of CSPI04G22420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L372	4.0e-54	100.00	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A0A0KZZ7	8.0e-47	88.99	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A5D3DVK8	2.0e-42	88.12	Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa...	[more]
A0A1S3CK11	2.0e-42	88.12	probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=...	[more]
A0A5D3DWN6	1.0e-41	87.13	Putative 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo var. makuwa...	[more]

BLAST of CSPI04G22420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146011.3	3.9e-48	99.00	LOW QUALITY PROTEIN: protein SRG1 [Cucumis sativus]	[more]
KGN55530.2	1.1e-42	88.12	hypothetical protein Csa_012011 [Cucumis sativus]	[more]
KAA0066697.1	4.2e-42	88.12	putative 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo var. makuwa] ...	[more]
XP_008463796.1	4.2e-42	88.12	PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo]	[more]
KAA0066698.1	2.1e-41	87.13	putative 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo var. makuwa]	[more]

BLAST of CSPI04G22420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25310.1	2.0e-29	61.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	5.7e-29	59.41	senescence-related gene 1	[more]
AT4G25300.1	7.0e-27	59.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25300.2	7.0e-27	59.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78550.1	1.6e-26	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 7..27
None	No IPR available	PANTHER	PTHR47991:SF126	2OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASE	coord: 10..109
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 10..109
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..109
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..110 e-value: 4.2E-28 score: 100.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 51..108 e-value: 1.2E-11 score: 45.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G22420.1	CSPI04G22420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding