CSPI04G21620 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G21620
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description3-hydroxyisobutyryl-CoA hydrolase-like protein 5
LocationChr4: 20094858 .. 20099146 (-)
RNA-Seq ExpressionCSPI04G21620
SyntenyCSPI04G21620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTAGTCGATTTTAGTTCTGTTTGCTTCACCAGCAGTAGCCACCTAAATATGACCTTAGGACTACAATTTCCTCTTTGGTTGGTGGGATTTTAATGGTTGGTGGTAGGTGGGAGGAGAAATGCTGTCGATTAGACTCTACTTTTGGTCTATTTTTAAGCAACTTCTTTGCAATTTCACTTGTTTTCACTGCTGATCATCTTTCATTTCACTCCGCTGCTCAGATTGAAAACCCGTCGGGCTTGGTTTTTTCAGTTCTTTCGATCTGCTGCTCTCATTTTGATTGCTCCTTTTGCTAGCTTCAATCCAGAATTTCGACTTCGGAATGGCGCTGGAAGTTGTTAAACCAGAGGAAGAGGTAGTTCTTTACTGCTTCTCCAACTTTCTGGAATGCGACCCTTTGTTTTTAATGTAATTTCTTTTAACCATTTTACTTGCTTCATTGTAGTTGAGAATTTTCCCCTCGGGTGAAATTTTGTGGGAGTTTCTCACTCTTTCATTCACTTAATGCTTGTCCGAATTCAAATTTTCAGGTTGTTCTGGGAGAAGAAGTTGGAAATGCCAGGTTGATTACGTTTAACCGGCCAAGGCAATTGAATGTCATCTCATCTAAAGTGGTGTGTAAAATTTTAACTCCATCTAACTGGATTATTGATTGGTCTTATGTTCTTGAATAAAGGGATGAACAAGGACTTTCCTCTCTATATCGTGTGTATTTAGGTTTCTCTGTTGGCTGGATACTTAGAGAAATGGGAAAAAGATGATGAAGCGAAACTTATAATCTTTAAGGTCAGTTCTGTCATCGATGCCTGATAGAAATATTGCTTAGTTGCAGTTGGTTTTGGCTCTTAGCTTTTCCAAACTGCAGGGATGATAAATAAGAAGTAAGAACCTTAACTCTTTGAATTGGGGTTTCGTTTTGTAAATTAGTCTTTGTCATGTGTCTTAAACAGGGAGCTGGTCGAGCCTTTTCTGCTGGTGGAGACTTAAGGATGTTCTATGAAGGCAGGAATAAAAGTAGGTGTTTGTTTTCTTTTTACCACATGAGTAAAATAGCTCTTTAAGAATAGTGAGTTGAGCAATGAGCATAATACGAGAAGTGAAGCTTCAATGCACATGGAAAATCGTACATGTTACAATTAGGTTTGGTAACTATTTCATTTTTTCATTATAAAAATTAGGCCTATTTTCTTCCAATTTCTTACAATGATTGATATCTTACAAATACATGTGTTGTATTCTTAGTCAAATTTCAAAAGCAAAAACTACTTCCTTTAGTTTCTAAATTTTGGCTTCGTTTTTTTTTTTTAAACAATGATAAAACTGAAATGTAGATAGAAAAAGCAAGATATTTAGTGATGAAAGATGTGTTTATAGGTTTAATTTTCAAGAACTAAAAACCAAAAACCAAATAGTTACCAAACTTGGCTTCTATCCATCCCCAACCATCATGGTTGGCCCAACTGTAGAAAATGAGACAGTCTACATTGTCACGTTCAACAGTGTGGATCCCATTCTTTATCAGAATGAGAACAACTAATTCAATACTTAAATTTCAATGATCACAAAGTTAAGTTCCTAATGCATTCTTTTTAACAGAGGATTCATGCCTTGAAGTTGTCTACCGAATGTATTGGCTCTGCTATCATGTTCACACCTATAAGAAAATCCACGTGAGGATTTCTCTGATCCCATTATACAAGAATTGTGGGTCTTTTTTCCTTAAAAAGTTCGATAAAGTTAAATGTTTGGCTCCTGTTCTGCTCATCTGATCACAGGTTGCACTTGTACACGGAATTGCAATGGGAGGAGGCGCATCATTTATGGTGCCAATGAAATTTTCAGTCGTCACAGAGAAAACTGTGGGTTTTTCTTTGTTTTTTTGTCTGCCTTCTTAAATGTTAACGTAATTTGCATGTTTGTTTCAGTTTCCATAGTAATTCTGCAGTGATTTCTGTCATGGATTCTTTTATATAGGTTTTTGCCACTCCAGAAGCAAGTATTGGATTTCACACTGACTGTGGTTTCTCGTATATGTTTTCTCGCCTTCCGGGATATTTAGGTAACAGTGCAGTGAGAAAAGTTCATTGGATGGTTTAAAAACATATCGACTGATTTAATAAGGATGTGGACATATACAGGTGAATTCTTGGCCTTGACTGGGTCCAGGTTGAACGGCAAAGAATTGGTTGCAGTTGGATTTGCAACACATTTTGTCCCTTCTGAGGTAAGTTTTGCGATCTTCTATTAGGAGTTTCAATTTCAAGATTTTGTTGACTAATATTAAATGCAATTAGTAAAAATAATTAATTCATTTTACAAGGGAAGAAAAATACAATCCAGGCGATGAAGGCACTAGAAATAATATAGAGCATATTACTTTTCCTTAGTTTTTCGTTCTTTACATGAAGTTTGTTCCCGTATTTTCAATTCAAATTTATGTCTCTGAAGCAGCATCCAGAAGCTTACATGTATAAGAAATAAAAATTACCACCATCTATAATCGATAAGGTTCCACTTTCTTTCAATTATGCGAGGTAGTAAAAAGCATCATTTATAGTCGAGTTTTATTTAGTTTTGATTTCACACTTTGAGTTTGCTTCAGTACTGCTGCATTGTTCTATGTGAAAGACGAAGCCTTCATCTGTTTATTGCAGAAATTGGATGAACTAGAGACACGTTTGGTTAGTTTGAACTCCCATGATGAAAATTCAGTTCGATCTGCAATTGAAGATTTTTCTTTAGAAGTTCAGCTGGATGAAGATAGTGTCTTGAAAAGGTTGTGAATGGAATTAGATGAAGAGTTTCCTTTTCATACTGAATATCTAATGTATTTGAGATCATAATGAGTCAAATACCTGAAGTTAACTGCTGGGAATATTCCAGGAAATCCGTGATTGACAAGTGCTTTTCAAAGGAAACTGTTGAAGAGATAATAACCTCCTTTGTAAGTCTCTCTTTCTCTCCCTACCCTGCTGTTTTCTCTCTTTCTCTGAGCATCTGGGCTTCTATAAATCTGTGAACATTTATTTTGTTCAGGAGGCCGAGGCAAGCGTTGAAGGAAATGGTTGGATAGTTCCCGTCCTGAAGGGACTAAAGAGATCATCCCCTACAGGACTGAAAATAACTCTAAAATCGGTACCACATCCAAACTCTTACACACGAATGCAAATGATTCACTTCTTCTTGACAACGTTATCCCATTCCTTAATTTTCAGAACAGACTTCAAAATTTCCATTCACTTGAAGATTCTTTGCTTACTATCAGTTTTATTTGTAACTTAGCAGTATCAAATGGGAAATTTCTGGAAACTTTTTACTTTCAACTGCTGTCTTCTACTATTGTGAACTCTCCATGTTATTTCTTTTATCCAACCCGTGATGGCTGTAGTAGCTTGTCTTGTGTGTTTGCTGCTAATTTCTCACTTCACCATTTCCAGATACGCAAAGGTCGATTACTAACACTGCAAGAATGTTTGAAGAAGGAATTCAGGCTGACAATGAACATTCTACGCACCCTTATCTCTGGAGATGTTTATGAGGTAATACGATCATTGATAGCCAATATAATCGTAAGATGATAATCACAAGCATAATAGACGTGGCCACTTCTAAACTAATCTTTTTCCTGTTGACCAAAGGGTATTAGAGCTCTCACCATCGACAAAGACAATGCTCCTAAGGTTTTCTCTTCTCTATCTTTGTGTGCAACACAAATACACAAATTGTTTCATTCTGTCTTCTTAACTAAAAATTATTAAACTGGATACAGTGGGACCCACCATCTCTTGAGAAGGTTAACGACGATAGGTTAAATGAAGTATTTCAGCCTTACGGGGAAGATTTGGAACTCCAAGTACCCATAGATGACGAACAAAGGTTGGTACCAATTACAATCTCAGCTTCTAACAGCCAACTTTAGTGAGCCATATTGATGAAGAATATGATAAGTTTGTTTGTTTGTTTTTCATTCGAAACCAGGTGGAGTGGTAAATATGAAGATTCAATTTATGGAACTATGAAAACGGAGTGAGTAATGCAATTGCAATACAAAGTTTCTCTCAAAGTTTCTCTCATGTTCTGTAGTAGCTGTTATGGTTGGAATAATCTTCTTTTATCAGCTGCCTCATTACGCCTATCATAACAATTCCCTTTATAATCCATTACATATTTACATGCAAAGCAGTTATTTACAAATTTTGTTGTAGTAATGTTATTTTGTTTACCGAACTTTACACGGTAAATCACAACATAATAATAAATAAAGCTATTTTCAAATATAGCAAGATA

mRNA sequence

TGGTAGTCGATTTTAGTTCTGTTTGCTTCACCAGCAGTAGCCACCTAAATATGACCTTAGGACTACAATTTCCTCTTTGGTTGGTGGGATTTTAATGGTTGGTGGTAGGTGGGAGGAGAAATGCTGTCGATTAGACTCTACTTTTGGTCTATTTTTAAGCAACTTCTTTGCAATTTCACTTGTTTTCACTGCTGATCATCTTTCATTTCACTCCGCTGCTCAGATTGAAAACCCGTCGGGCTTGGTTTTTTCAGTTCTTTCGATCTGCTGCTCTCATTTTGATTGCTCCTTTTGCTAGCTTCAATCCAGAATTTCGACTTCGGAATGGCGCTGGAAGTTGTTAAACCAGAGGAAGAGGTTGTTCTGGGAGAAGAAGTTGGAAATGCCAGGTTGATTACGTTTAACCGGCCAAGGCAATTGAATGTCATCTCATCTAAAGTGGTTTCTCTGTTGGCTGGATACTTAGAGAAATGGGAAAAAGATGATGAAGCGAAACTTATAATCTTTAAGGGAGCTGGTCGAGCCTTTTCTGCTGGTGGAGACTTAAGGATGTTCTATGAAGGCAGGAATAAAAAGGATTCATGCCTTGAAGTTGTCTACCGAATGTATTGGCTCTGCTATCATGTTCACACCTATAAGAAAATCCACGTTGCACTTGTACACGGAATTGCAATGGGAGGAGGCGCATCATTTATGGTGCCAATGAAATTTTCAGTCGTCACAGAGAAAACTGTTTTTGCCACTCCAGAAGCAAGTATTGGATTTCACACTGACTGTGGTTTCTCGTATATGTTTTCTCGCCTTCCGGGATATTTAGGTGAATTCTTGGCCTTGACTGGGTCCAGGTTGAACGGCAAAGAATTGGTTGCAGTTGGATTTGCAACACATTTTGTCCCTTCTGAGAAATTGGATGAACTAGAGACACGTTTGGTTAGTTTGAACTCCCATGATGAAAATTCAGTTCGATCTGCAATTGAAGATTTTTCTTTAGAAGTTCAGCTGGATGAAGATAGTGTCTTGAAAAGGAAATCCGTGATTGACAAGTGCTTTTCAAAGGAAACTGTTGAAGAGATAATAACCTCCTTTGAGGCCGAGGCAAGCGTTGAAGGAAATGGTTGGATAGTTCCCGTCCTGAAGGGACTAAAGAGATCATCCCCTACAGGACTGAAAATAACTCTAAAATCGATACGCAAAGGTCGATTACTAACACTGCAAGAATGTTTGAAGAAGGAATTCAGGCTGACAATGAACATTCTACGCACCCTTATCTCTGGAGATGTTTATGAGGGTATTAGAGCTCTCACCATCGACAAAGACAATGCTCCTAAGTGGGACCCACCATCTCTTGAGAAGGTTAACGACGATAGGTTAAATGAAGTATTTCAGCCTTACGGGGAAGATTTGGAACTCCAAGTACCCATAGATGACGAACAAAGGTGGAGTGGTAAATATGAAGATTCAATTTATGGAACTATGAAAACGGAGTGAGTAATGCAATTGCAATACAAAGTTTCTCTCAAAGTTTCTCTCATGTTCTGTAGTAGCTGTTATGGTTGGAATAATCTTCTTTTATCAGCTGCCTCATTACGCCTATCATAACAATTCCCTTTATAATCCATTACATATTTACATGCAAAGCAGTTATTTACAAATTTTGTTGTAGTAATGTTATTTTGTTTACCGAACTTTACACGGTAAATCACAACATAATAATAAATAAAGCTATTTTCAAATATAGCAAGATA

Coding sequence (CDS)

ATGGCGCTGGAAGTTGTTAAACCAGAGGAAGAGGTTGTTCTGGGAGAAGAAGTTGGAAATGCCAGGTTGATTACGTTTAACCGGCCAAGGCAATTGAATGTCATCTCATCTAAAGTGGTTTCTCTGTTGGCTGGATACTTAGAGAAATGGGAAAAAGATGATGAAGCGAAACTTATAATCTTTAAGGGAGCTGGTCGAGCCTTTTCTGCTGGTGGAGACTTAAGGATGTTCTATGAAGGCAGGAATAAAAAGGATTCATGCCTTGAAGTTGTCTACCGAATGTATTGGCTCTGCTATCATGTTCACACCTATAAGAAAATCCACGTTGCACTTGTACACGGAATTGCAATGGGAGGAGGCGCATCATTTATGGTGCCAATGAAATTTTCAGTCGTCACAGAGAAAACTGTTTTTGCCACTCCAGAAGCAAGTATTGGATTTCACACTGACTGTGGTTTCTCGTATATGTTTTCTCGCCTTCCGGGATATTTAGGTGAATTCTTGGCCTTGACTGGGTCCAGGTTGAACGGCAAAGAATTGGTTGCAGTTGGATTTGCAACACATTTTGTCCCTTCTGAGAAATTGGATGAACTAGAGACACGTTTGGTTAGTTTGAACTCCCATGATGAAAATTCAGTTCGATCTGCAATTGAAGATTTTTCTTTAGAAGTTCAGCTGGATGAAGATAGTGTCTTGAAAAGGAAATCCGTGATTGACAAGTGCTTTTCAAAGGAAACTGTTGAAGAGATAATAACCTCCTTTGAGGCCGAGGCAAGCGTTGAAGGAAATGGTTGGATAGTTCCCGTCCTGAAGGGACTAAAGAGATCATCCCCTACAGGACTGAAAATAACTCTAAAATCGATACGCAAAGGTCGATTACTAACACTGCAAGAATGTTTGAAGAAGGAATTCAGGCTGACAATGAACATTCTACGCACCCTTATCTCTGGAGATGTTTATGAGGGTATTAGAGCTCTCACCATCGACAAAGACAATGCTCCTAAGTGGGACCCACCATCTCTTGAGAAGGTTAACGACGATAGGTTAAATGAAGTATTTCAGCCTTACGGGGAAGATTTGGAACTCCAAGTACCCATAGATGACGAACAAAGGTGGAGTGGTAAATATGAAGATTCAATTTATGGAACTATGAAAACGGAGTGA

Protein sequence

MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQRWSGKYEDSIYGTMKTE*
Homology
BLAST of CSPI04G21620 vs. ExPASy Swiss-Prot
Match: Q9SHJ8 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At1g06550 PE=1 SV=2)

HSP 1 Score: 541.2 bits (1393), Expect = 9.6e-153
Identity = 267/386 (69.17%), Postives = 326/386 (84.46%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MA E    +E VV+GEE G+ RL T NRPRQLNVIS +VV  LA YLE WEKDD+ KLI+
Sbjct: 1   MAQEGQNIDEPVVIGEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLIL 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
            KG GRAFSAGGDL++FY G+  KDSCLEVVYRMYWLCYH+HTYKK  V+LV+GI+MGGG
Sbjct: 61  IKGTGRAFSAGGDLKVFYHGQESKDSCLEVVYRMYWLCYHIHTYKKTQVSLVNGISMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           A+ MVPMKFSVVTEKTVFATPEAS GFHTDCGFSY+ SRLPG+LGEFLALTG+RLNGKEL
Sbjct: 121 AALMVPMKFSVVTEKTVFATPEASFGFHTDCGFSYIHSRLPGHLGEFLALTGARLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VA+G ATHFVPS KL +LE RLVSL+S D + V+S IE+FS +V LD+DS+L ++SVI++
Sbjct: 181 VAIGMATHFVPSGKLMDLEARLVSLDSGDADVVQSTIEEFSEKVNLDKDSILNKQSVINE 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKE+V++II +FEAEAS +GN WI PV+KGLKRSSPTGLKI L+SIR+GR  TL +CL
Sbjct: 241 CFSKESVKQIIQAFEAEASKDGNEWITPVIKGLKRSSPTGLKIVLQSIREGRKQTLSDCL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPY-GED 360
           KKEFRLT+NILR  IS D+YEGIRALTIDKDN+PKW+P +L++V+D+++N VF+ +  +D
Sbjct: 301 KKEFRLTLNILRKTISPDMYEGIRALTIDKDNSPKWNPATLDEVDDEKINSVFKLFEDDD 360

Query: 361 LELQVPIDDEQRWSGKYEDSIYGTMK 386
           +ELQ+P  +E RW GKYE S Y +++
Sbjct: 361 IELQIPETEENRWGGKYETSGYASVR 386

BLAST of CSPI04G21620 vs. ExPASy Swiss-Prot
Match: Q9LKJ1 (3-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.5e-78
Identity = 155/367 (42.23%), Postives = 230/367 (62.67%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MA+E+    +  VL EE  + R++T NRP+QLN +S  ++S L      +E+D   KL+I
Sbjct: 1   MAVEMASQSQ--VLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVI 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVY--RMYWLCYHVHTYKKIHVALVHGIAMG 120
            KG GRAF AGGD+       N+ +  L   Y    Y L Y + TY K  V++++GI MG
Sbjct: 61  LKGHGRAFCAGGDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMG 120

Query: 121 GGASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGK 180
           GGA   V  +F + TE TVFA PE ++G   D G SY  SRLPG+ GE++ LTG+RL+G 
Sbjct: 121 GGAGVSVHGRFRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGA 180

Query: 181 ELVAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVI 240
           E++A G ATHFVPS +L  LE  L  +NS+D     + ++ ++   +L + S  +R  VI
Sbjct: 181 EMLACGLATHFVPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVI 240

Query: 241 DKCFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQE 300
           D+CFS+ TVEEII++ E EA+ E +GWI   ++ LK+ SP  LKI+L+SIR+GRL  + +
Sbjct: 241 DRCFSRRTVEEIISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQ 300

Query: 301 CLKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGE 360
           CL +E+R+  ++++  IS D  EG RA+ +DKD  PKW+P  LE + D  + + F+    
Sbjct: 301 CLIREYRMVCHVMKGEISKDFVEGCRAILVDKDKNPKWEPRRLEDMKDSMVEQYFERVER 360

Query: 361 DLELQVP 366
           + +L++P
Sbjct: 361 EDDLKLP 365

BLAST of CSPI04G21620 vs. ExPASy Swiss-Prot
Match: Q5XF59 (3-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g60510 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-76
Identity = 155/359 (43.18%), Postives = 223/359 (62.12%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL E  G +R    NRP  LN +++ +   L    + WE+D     ++ KG+GRAF AGG
Sbjct: 39  VLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSGRAFCAGG 98

Query: 73  DLRMFY--EGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFS 132
           D+   Y    R   D+  E    +Y   Y + TY K HVA+++G+ MGGG    +P  F 
Sbjct: 99  DIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGVSIPGTFR 158

Query: 133 VVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFV 192
           V T++T+FATPE  IGFH D G S+  S LPG LGE+L LTG +L+G E++A G ATH++
Sbjct: 159 VATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLACGLATHYI 218

Query: 193 PSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEI 252
            SE++  +E +L  L + D + V S +E  +     ++  V++R  +++KCFS +TVEEI
Sbjct: 219 RSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDLLEKCFSHDTVEEI 278

Query: 253 ITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNI 312
           I S E EAS   + W +  L+ LK SSP  LK+ L+SIR+GRL TL +CL +E+R+++  
Sbjct: 279 IDSLEIEASRRKDTWCITTLRRLKESSPLSLKVALRSIREGRLQTLDQCLIREYRMSLQG 338

Query: 313 LRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVF---QPYGEDLELQVPI 367
           L   +SG+  EG+RA  IDKD APKWDPPSLEKV++D +++ F    P   DL+L V +
Sbjct: 339 LIGPMSGNFCEGVRARLIDKDEAPKWDPPSLEKVSEDMVDDYFCALTPTEPDLDLPVKL 397

BLAST of CSPI04G21620 vs. ExPASy Swiss-Prot
Match: Q6NMB0 (Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g30660 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 8.1e-75
Identity = 149/362 (41.16%), Postives = 222/362 (61.33%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL EE  + R++T NRP+QLN +   ++S L      +E+D   KL+I KG GRAF AGG
Sbjct: 7   VLVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAGG 66

Query: 73  DLRMFYEG--RNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFS 132
           D+    +   + K     +     Y L Y + TY K  V++++GI MG GA   +  +F 
Sbjct: 67  DVPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNGIVMGAGAGVSIHGRFR 126

Query: 133 VVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFV 192
           + TE TVFA PE S+G   D G SY  SRLPG+ GE++ LTG+RL+G EL+A G ATHFV
Sbjct: 127 IATENTVFAMPETSLGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAELLACGLATHFV 186

Query: 193 PSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEI 252
           PS +L  LET L  + S D + V + ++ ++    L + S   R  VID+CFSK T+EEI
Sbjct: 187 PSTRLTALETDLCKVGSSDPSFVSTILDAYTQHPHLKQKSAYHRLDVIDRCFSKRTMEEI 246

Query: 253 ITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNI 312
           I++ E E + E + W +  ++ LK+SSP+ LKI+L+SIR+GRL  +  CL +E+R+  ++
Sbjct: 247 ISALERETTQELDDWSLTTIRALKKSSPSSLKISLRSIREGRLQGVGHCLTREYRMVCHV 306

Query: 313 LRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQ 372
           ++  +S D+ EG RA+ IDKD  PKW+P  LE + D  +++ F+           +++E+
Sbjct: 307 MKGDLSKDLVEGCRAILIDKDRNPKWEPRRLEDMKDSMVDQFFE----------RVEEEE 358

BLAST of CSPI04G21620 vs. ExPASy Swiss-Prot
Match: Q1PEY5 (Probable 3-hydroxyisobutyryl-CoA hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=At2g30650 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-73
Identity = 150/364 (41.21%), Postives = 226/364 (62.09%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL EE  + R++TFNRP+QLN +S  +VS L      +E+D   KL++ KG GRAFSAGG
Sbjct: 7   VLVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAGG 66

Query: 73  DL----RMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMK 132
           D+    R   +G+  + +    V   Y L Y + TY+K  V++++GI MGGGA      +
Sbjct: 67  DIPPIVRDILQGKLIRGAHYFKV--GYTLNYVLSTYRKPQVSILNGIVMGGGAGLSTNGR 126

Query: 133 FSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATH 192
           F + TE TVFA PE ++G   D G SY  SRLPG+ GE++ LTG+RL+G E++A G ATH
Sbjct: 127 FRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATH 186

Query: 193 FVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVE 252
           FVPS  L  LE  L  + S ++  + + ++ ++    L++ S   R  VID+CFSK TVE
Sbjct: 187 FVPSISLTALEAELYKVGSSNQTFISTILDAYAEYPHLNQHSSYHRLDVIDRCFSKRTVE 246

Query: 253 EIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTM 312
           EI ++ E E + + N W++  ++ L+++SP+ LKI+L+SIR+GRL  + +CL +E+R+  
Sbjct: 247 EIFSALEREVTQKPNDWLLATIQALEKASPSCLKISLRSIREGRLQGVGQCLIREYRMVC 306

Query: 313 NILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDD 372
           ++++  IS D  EG RA+ IDKD  PKW P  LE V D  +++ F+           ++D
Sbjct: 307 HVMKGDISKDFVEGCRAVLIDKDRNPKWQPRRLEDVTDSMVDQYFE----------RVED 358

BLAST of CSPI04G21620 vs. ExPASy TrEMBL
Match: A0A0A0KZ34 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G608110 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.0e-218
Identity = 387/387 (100.00%), Postives = 387/387 (100.00%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
           FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK
Sbjct: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL
Sbjct: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL
Sbjct: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPIDDEQRWSGKYEDSIYGTMKTE
Sbjct: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 387

BLAST of CSPI04G21620 vs. ExPASy TrEMBL
Match: A0A5A7UF77 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504G00450 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.5e-212
Identity = 371/387 (95.87%), Postives = 382/387 (98.71%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGN RLIT NRPRQLNVISSKVVSLLAGYLEKWEKDDE KLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNVRLITLNRPRQLNVISSKVVSLLAGYLEKWEKDDEGKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
           FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKK+HVALVHGIAMGGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKMHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKT+FATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTIFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVPS+KLDELETRL+SLNSHDENSVRSAIEDFSLEVQLDEDSVLKR+SVIDK
Sbjct: 181 VAVGFATHFVPSDKLDELETRLISLNSHDENSVRSAIEDFSLEVQLDEDSVLKRQSVIDK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEIITSFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL
Sbjct: 241 CFSKETVEEIITSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSL+KV+DD++NEVFQPYGEDL
Sbjct: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLDKVDDDKVNEVFQPYGEDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPIDDEQRWSGKYEDSIY T+K E
Sbjct: 361 ELQVPIDDEQRWSGKYEDSIYATLKME 387

BLAST of CSPI04G21620 vs. ExPASy TrEMBL
Match: A0A1S3CNX2 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503054 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.5e-212
Identity = 371/387 (95.87%), Postives = 382/387 (98.71%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGN RLIT NRPRQLNVISSKVVSLLAGYLEKWEKDDE KLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNVRLITLNRPRQLNVISSKVVSLLAGYLEKWEKDDEGKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
           FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKK+HVALVHGIAMGGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKMHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKT+FATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTIFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVPS+KLDELETRL+SLNSHDENSVRSAIEDFSLEVQLDEDSVLKR+SVIDK
Sbjct: 181 VAVGFATHFVPSDKLDELETRLISLNSHDENSVRSAIEDFSLEVQLDEDSVLKRQSVIDK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEIITSFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL
Sbjct: 241 CFSKETVEEIITSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSL+KV+DD++NEVFQPYGEDL
Sbjct: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLDKVDDDKVNEVFQPYGEDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPIDDEQRWSGKYEDSIY T+K E
Sbjct: 361 ELQVPIDDEQRWSGKYEDSIYATLKME 387

BLAST of CSPI04G21620 vs. ExPASy TrEMBL
Match: A0A1S3CNX6 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503054 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 6.8e-210
Identity = 370/388 (95.36%), Postives = 382/388 (98.45%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGN RLIT NRPRQLNVISSKVVSLLAGYLEKWEKDDE KLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNVRLITLNRPRQLNVISSKVVSLLAGYLEKWEKDDEGKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNK-KDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGG 120
           FKGAGRAFSAGGDLRMFYEGRNK +DSCLEVVYRMYWLCYHVHTYKK+HVALVHGIAMGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKTEDSCLEVVYRMYWLCYHVHTYKKMHVALVHGIAMGG 120

Query: 121 GASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE 180
           GASFMVPMKFSVVTEKT+FATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE
Sbjct: 121 GASFMVPMKFSVVTEKTIFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE 180

Query: 181 LVAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVID 240
           LVAVGFATHFVPS+KLDELETRL+SLNSHDENSVRSAIEDFSLEVQLDEDSVLKR+SVID
Sbjct: 181 LVAVGFATHFVPSDKLDELETRLISLNSHDENSVRSAIEDFSLEVQLDEDSVLKRQSVID 240

Query: 241 KCFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC 300
           KCFSKETVEEIITSFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC
Sbjct: 241 KCFSKETVEEIITSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC 300

Query: 301 LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGED 360
           LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSL+KV+DD++NEVFQPYGED
Sbjct: 301 LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLDKVDDDKVNEVFQPYGED 360

Query: 361 LELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           LELQVPIDDEQRWSGKYEDSIY T+K E
Sbjct: 361 LELQVPIDDEQRWSGKYEDSIYATLKME 388

BLAST of CSPI04G21620 vs. ExPASy TrEMBL
Match: A0A6J1CL87 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111012459 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.0e-189
Identity = 330/387 (85.27%), Postives = 359/387 (92.76%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MA +VV P+ EVVLGEE+ + RL+T NRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLI+
Sbjct: 53  MAQQVVNPDGEVVLGEEIEHVRLVTLNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIV 112

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
            KG+GRAFSAGGDL+MFY+GRN KDSCLEVVYRMYWLCYH+HTYKKIHVALVHGI+MGGG
Sbjct: 113 IKGSGRAFSAGGDLKMFYDGRNTKDSCLEVVYRMYWLCYHIHTYKKIHVALVHGISMGGG 172

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEK VFATPEASIGFHTDCGFSY+ S LPGYLGE+LALTGSRLNGKEL
Sbjct: 173 ASFMVPMKFSVVTEKAVFATPEASIGFHTDCGFSYILSHLPGYLGEYLALTGSRLNGKEL 232

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VA G ATHFVP E+L ELE RL+SLNS+DENS+RSAIE+FS EV+LD DSVLKR+SVIDK
Sbjct: 233 VAAGLATHFVPYEQLGELEKRLISLNSYDENSIRSAIEEFSSEVELDGDSVLKRQSVIDK 292

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETV EII SFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGR  TLQECL
Sbjct: 293 CFSKETVAEIINSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRTQTLQECL 352

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRT+ISGDVYEGIRALTIDKDNAPKWDPP+LEKV DD++NEVFQP+ EDL
Sbjct: 353 KKEFRLTMNILRTIISGDVYEGIRALTIDKDNAPKWDPPTLEKVEDDKVNEVFQPFREDL 412

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVP +DE RWSGKYEDS+Y T+K E
Sbjct: 413 ELQVPKNDEHRWSGKYEDSMYSTVKVE 439

BLAST of CSPI04G21620 vs. NCBI nr
Match: XP_004144631.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis sativus] >XP_011653977.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis sativus] >XP_031739895.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis sativus] >KGN54935.1 hypothetical protein Csa_012690 [Cucumis sativus])

HSP 1 Score: 767.3 bits (1980), Expect = 6.3e-218
Identity = 387/387 (100.00%), Postives = 387/387 (100.00%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
           FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK
Sbjct: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL
Sbjct: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL
Sbjct: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPIDDEQRWSGKYEDSIYGTMKTE
Sbjct: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 387

BLAST of CSPI04G21620 vs. NCBI nr
Match: XP_031739896.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis sativus])

HSP 1 Score: 747.7 bits (1929), Expect = 5.2e-212
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 12  VVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAG 71
           VVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAG
Sbjct: 8   VVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAG 67

Query: 72  GDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFSV 131
           GDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFSV
Sbjct: 68  GDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFSV 127

Query: 132 VTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFVP 191
           VTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFVP
Sbjct: 128 VTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFVP 187

Query: 192 SEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEII 251
           SEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEII
Sbjct: 188 SEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEII 247

Query: 252 TSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNIL 311
           TSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNIL
Sbjct: 248 TSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNIL 307

Query: 312 RTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQR 371
           RTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQR
Sbjct: 308 RTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQR 367

Query: 372 WSGKYEDSIYGTMKTE 388
           WSGKYEDSIYGTMKTE
Sbjct: 368 WSGKYEDSIYGTMKTE 383

BLAST of CSPI04G21620 vs. NCBI nr
Match: XP_008465449.1 (PREDICTED: 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis melo] >KAA0052375.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis melo var. makuwa] >TYK09413.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 745.7 bits (1924), Expect = 2.0e-211
Identity = 371/387 (95.87%), Postives = 382/387 (98.71%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGN RLIT NRPRQLNVISSKVVSLLAGYLEKWEKDDE KLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNVRLITLNRPRQLNVISSKVVSLLAGYLEKWEKDDEGKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
           FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKK+HVALVHGIAMGGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKMHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKT+FATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTIFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVPS+KLDELETRL+SLNSHDENSVRSAIEDFSLEVQLDEDSVLKR+SVIDK
Sbjct: 181 VAVGFATHFVPSDKLDELETRLISLNSHDENSVRSAIEDFSLEVQLDEDSVLKRQSVIDK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEIITSFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL
Sbjct: 241 CFSKETVEEIITSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSL+KV+DD++NEVFQPYGEDL
Sbjct: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLDKVDDDKVNEVFQPYGEDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPIDDEQRWSGKYEDSIY T+K E
Sbjct: 361 ELQVPIDDEQRWSGKYEDSIYATLKME 387

BLAST of CSPI04G21620 vs. NCBI nr
Match: XP_008465447.1 (PREDICTED: 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis melo])

HSP 1 Score: 739.6 bits (1908), Expect = 1.4e-209
Identity = 370/388 (95.36%), Postives = 382/388 (98.45%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVVKPEEEVVLGEEVGN RLIT NRPRQLNVISSKVVSLLAGYLEKWEKDDE KLII
Sbjct: 1   MALEVVKPEEEVVLGEEVGNVRLITLNRPRQLNVISSKVVSLLAGYLEKWEKDDEGKLII 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNK-KDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGG 120
           FKGAGRAFSAGGDLRMFYEGRNK +DSCLEVVYRMYWLCYHVHTYKK+HVALVHGIAMGG
Sbjct: 61  FKGAGRAFSAGGDLRMFYEGRNKTEDSCLEVVYRMYWLCYHVHTYKKMHVALVHGIAMGG 120

Query: 121 GASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE 180
           GASFMVPMKFSVVTEKT+FATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE
Sbjct: 121 GASFMVPMKFSVVTEKTIFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKE 180

Query: 181 LVAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVID 240
           LVAVGFATHFVPS+KLDELETRL+SLNSHDENSVRSAIEDFSLEVQLDEDSVLKR+SVID
Sbjct: 181 LVAVGFATHFVPSDKLDELETRLISLNSHDENSVRSAIEDFSLEVQLDEDSVLKRQSVID 240

Query: 241 KCFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC 300
           KCFSKETVEEIITSFEAEASVEGNGWI+PVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC
Sbjct: 241 KCFSKETVEEIITSFEAEASVEGNGWIIPVLKGLKRSSPTGLKITLKSIRKGRLLTLQEC 300

Query: 301 LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGED 360
           LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSL+KV+DD++NEVFQPYGED
Sbjct: 301 LKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLDKVDDDKVNEVFQPYGED 360

Query: 361 LELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           LELQVPIDDEQRWSGKYEDSIY T+K E
Sbjct: 361 LELQVPIDDEQRWSGKYEDSIYATLKME 388

BLAST of CSPI04G21620 vs. NCBI nr
Match: XP_038896721.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida] >XP_038896722.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida] >XP_038896723.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida] >XP_038896724.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida] >XP_038896725.1 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida])

HSP 1 Score: 701.0 bits (1808), Expect = 5.5e-198
Identity = 353/387 (91.21%), Postives = 371/387 (95.87%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MALEVV P+E+VVLG EVGN RLITFNRP+QLNVISSKVVSLLAG LEKWEKDDEAKLI+
Sbjct: 1   MALEVVNPDEQVVLG-EVGNVRLITFNRPKQLNVISSKVVSLLAGCLEKWEKDDEAKLIL 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
            KGAGRAFSAGGDLRMFYEGRN KDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG
Sbjct: 61  IKGAGRAFSAGGDLRMFYEGRNSKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGE+LALTGSRLNGKEL
Sbjct: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEYLALTGSRLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VAVGFATHFVP+EKL ELE RL+SLNSHDENSVRSAIE+FSLEVQLDE+SVLKR+ VI+K
Sbjct: 181 VAVGFATHFVPTEKLGELEKRLISLNSHDENSVRSAIEEFSLEVQLDENSVLKRQFVINK 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKETVEEII SFEAEASVEGN WIVPVLKGLKRSSPTGLKITLKSIRKGR LTLQECL
Sbjct: 241 CFSKETVEEIIKSFEAEASVEGNSWIVPVLKGLKRSSPTGLKITLKSIRKGRSLTLQECL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDL 360
           KKEFRLTMNIL TLISGDVYEGIRALTIDKDNAPKW+PPSLEKV D+++NEVFQPYG+DL
Sbjct: 301 KKEFRLTMNILLTLISGDVYEGIRALTIDKDNAPKWNPPSLEKVEDEKVNEVFQPYGKDL 360

Query: 361 ELQVPIDDEQRWSGKYEDSIYGTMKTE 388
           ELQVPI+DEQRWSGKYEDSIY T+K E
Sbjct: 361 ELQVPINDEQRWSGKYEDSIYATVKME 386

BLAST of CSPI04G21620 vs. TAIR 10
Match: AT1G06550.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 541.2 bits (1393), Expect = 6.8e-154
Identity = 267/386 (69.17%), Postives = 326/386 (84.46%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MA E    +E VV+GEE G+ RL T NRPRQLNVIS +VV  LA YLE WEKDD+ KLI+
Sbjct: 1   MAQEGQNIDEPVVIGEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLIL 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGG 120
            KG GRAFSAGGDL++FY G+  KDSCLEVVYRMYWLCYH+HTYKK  V+LV+GI+MGGG
Sbjct: 61  IKGTGRAFSAGGDLKVFYHGQESKDSCLEVVYRMYWLCYHIHTYKKTQVSLVNGISMGGG 120

Query: 121 ASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKEL 180
           A+ MVPMKFSVVTEKTVFATPEAS GFHTDCGFSY+ SRLPG+LGEFLALTG+RLNGKEL
Sbjct: 121 AALMVPMKFSVVTEKTVFATPEASFGFHTDCGFSYIHSRLPGHLGEFLALTGARLNGKEL 180

Query: 181 VAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDK 240
           VA+G ATHFVPS KL +LE RLVSL+S D + V+S IE+FS +V LD+DS+L ++SVI++
Sbjct: 181 VAIGMATHFVPSGKLMDLEARLVSLDSGDADVVQSTIEEFSEKVNLDKDSILNKQSVINE 240

Query: 241 CFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECL 300
           CFSKE+V++II +FEAEAS +GN WI PV+KGLKRSSPTGLKI L+SIR+GR  TL +CL
Sbjct: 241 CFSKESVKQIIQAFEAEASKDGNEWITPVIKGLKRSSPTGLKIVLQSIREGRKQTLSDCL 300

Query: 301 KKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPY-GED 360
           KKEFRLT+NILR  IS D+YEGIRALTIDKDN+PKW+P +L++V+D+++N VF+ +  +D
Sbjct: 301 KKEFRLTLNILRKTISPDMYEGIRALTIDKDNSPKWNPATLDEVDDEKINSVFKLFEDDD 360

Query: 361 LELQVPIDDEQRWSGKYEDSIYGTMK 386
           +ELQ+P  +E RW GKYE S Y +++
Sbjct: 361 IELQIPETEENRWGGKYETSGYASVR 386

BLAST of CSPI04G21620 vs. TAIR 10
Match: AT5G65940.1 (beta-hydroxyisobutyryl-CoA hydrolase 1 )

HSP 1 Score: 293.5 bits (750), Expect = 2.5e-79
Identity = 155/367 (42.23%), Postives = 230/367 (62.67%), Query Frame = 0

Query: 1   MALEVVKPEEEVVLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLII 60
           MA+E+    +  VL EE  + R++T NRP+QLN +S  ++S L      +E+D   KL+I
Sbjct: 1   MAVEMASQSQ--VLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVI 60

Query: 61  FKGAGRAFSAGGDLRMFYEGRNKKDSCLEVVY--RMYWLCYHVHTYKKIHVALVHGIAMG 120
            KG GRAF AGGD+       N+ +  L   Y    Y L Y + TY K  V++++GI MG
Sbjct: 61  LKGHGRAFCAGGDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMG 120

Query: 121 GGASFMVPMKFSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGK 180
           GGA   V  +F + TE TVFA PE ++G   D G SY  SRLPG+ GE++ LTG+RL+G 
Sbjct: 121 GGAGVSVHGRFRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGA 180

Query: 181 ELVAVGFATHFVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVI 240
           E++A G ATHFVPS +L  LE  L  +NS+D     + ++ ++   +L + S  +R  VI
Sbjct: 181 EMLACGLATHFVPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVI 240

Query: 241 DKCFSKETVEEIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQE 300
           D+CFS+ TVEEII++ E EA+ E +GWI   ++ LK+ SP  LKI+L+SIR+GRL  + +
Sbjct: 241 DRCFSRRTVEEIISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQ 300

Query: 301 CLKKEFRLTMNILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGE 360
           CL +E+R+  ++++  IS D  EG RA+ +DKD  PKW+P  LE + D  + + F+    
Sbjct: 301 CLIREYRMVCHVMKGEISKDFVEGCRAILVDKDKNPKWEPRRLEDMKDSMVEQYFERVER 360

Query: 361 DLELQVP 366
           + +L++P
Sbjct: 361 EDDLKLP 365

BLAST of CSPI04G21620 vs. TAIR 10
Match: AT3G60510.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-77
Identity = 155/359 (43.18%), Postives = 223/359 (62.12%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL E  G +R    NRP  LN +++ +   L    + WE+D     ++ KG+GRAF AGG
Sbjct: 39  VLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSGRAFCAGG 98

Query: 73  DLRMFY--EGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFS 132
           D+   Y    R   D+  E    +Y   Y + TY K HVA+++G+ MGGG    +P  F 
Sbjct: 99  DIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGVSIPGTFR 158

Query: 133 VVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFV 192
           V T++T+FATPE  IGFH D G S+  S LPG LGE+L LTG +L+G E++A G ATH++
Sbjct: 159 VATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLACGLATHYI 218

Query: 193 PSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEI 252
            SE++  +E +L  L + D + V S +E  +     ++  V++R  +++KCFS +TVEEI
Sbjct: 219 RSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDLLEKCFSHDTVEEI 278

Query: 253 ITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNI 312
           I S E EAS   + W +  L+ LK SSP  LK+ L+SIR+GRL TL +CL +E+R+++  
Sbjct: 279 IDSLEIEASRRKDTWCITTLRRLKESSPLSLKVALRSIREGRLQTLDQCLIREYRMSLQG 338

Query: 313 LRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVF---QPYGEDLELQVPI 367
           L   +SG+  EG+RA  IDKD APKWDPPSLEKV++D +++ F    P   DL+L V +
Sbjct: 339 LIGPMSGNFCEGVRARLIDKDEAPKWDPPSLEKVSEDMVDDYFCALTPTEPDLDLPVKL 397

BLAST of CSPI04G21620 vs. TAIR 10
Match: AT2G30660.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.7e-76
Identity = 149/362 (41.16%), Postives = 222/362 (61.33%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL EE  + R++T NRP+QLN +   ++S L      +E+D   KL+I KG GRAF AGG
Sbjct: 7   VLVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAGG 66

Query: 73  DLRMFYEG--RNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMKFS 132
           D+    +   + K     +     Y L Y + TY K  V++++GI MG GA   +  +F 
Sbjct: 67  DVPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNGIVMGAGAGVSIHGRFR 126

Query: 133 VVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATHFV 192
           + TE TVFA PE S+G   D G SY  SRLPG+ GE++ LTG+RL+G EL+A G ATHFV
Sbjct: 127 IATENTVFAMPETSLGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAELLACGLATHFV 186

Query: 193 PSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVEEI 252
           PS +L  LET L  + S D + V + ++ ++    L + S   R  VID+CFSK T+EEI
Sbjct: 187 PSTRLTALETDLCKVGSSDPSFVSTILDAYTQHPHLKQKSAYHRLDVIDRCFSKRTMEEI 246

Query: 253 ITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTMNI 312
           I++ E E + E + W +  ++ LK+SSP+ LKI+L+SIR+GRL  +  CL +E+R+  ++
Sbjct: 247 ISALERETTQELDDWSLTTIRALKKSSPSSLKISLRSIREGRLQGVGHCLTREYRMVCHV 306

Query: 313 LRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDDEQ 372
           ++  +S D+ EG RA+ IDKD  PKW+P  LE + D  +++ F+           +++E+
Sbjct: 307 MKGDLSKDLVEGCRAILIDKDRNPKWEPRRLEDMKDSMVDQFFE----------RVEEEE 358

BLAST of CSPI04G21620 vs. TAIR 10
Match: AT2G30650.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 278.5 bits (711), Expect = 8.3e-75
Identity = 150/364 (41.21%), Postives = 226/364 (62.09%), Query Frame = 0

Query: 13  VLGEEVGNARLITFNRPRQLNVISSKVVSLLAGYLEKWEKDDEAKLIIFKGAGRAFSAGG 72
           VL EE  + R++TFNRP+QLN +S  +VS L      +E+D   KL++ KG GRAFSAGG
Sbjct: 7   VLVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAGG 66

Query: 73  DL----RMFYEGRNKKDSCLEVVYRMYWLCYHVHTYKKIHVALVHGIAMGGGASFMVPMK 132
           D+    R   +G+  + +    V   Y L Y + TY+K  V++++GI MGGGA      +
Sbjct: 67  DIPPIVRDILQGKLIRGAHYFKV--GYTLNYVLSTYRKPQVSILNGIVMGGGAGLSTNGR 126

Query: 133 FSVVTEKTVFATPEASIGFHTDCGFSYMFSRLPGYLGEFLALTGSRLNGKELVAVGFATH 192
           F + TE TVFA PE ++G   D G SY  SRLPG+ GE++ LTG+RL+G E++A G ATH
Sbjct: 127 FRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATH 186

Query: 193 FVPSEKLDELETRLVSLNSHDENSVRSAIEDFSLEVQLDEDSVLKRKSVIDKCFSKETVE 252
           FVPS  L  LE  L  + S ++  + + ++ ++    L++ S   R  VID+CFSK TVE
Sbjct: 187 FVPSISLTALEAELYKVGSSNQTFISTILDAYAEYPHLNQHSSYHRLDVIDRCFSKRTVE 246

Query: 253 EIITSFEAEASVEGNGWIVPVLKGLKRSSPTGLKITLKSIRKGRLLTLQECLKKEFRLTM 312
           EI ++ E E + + N W++  ++ L+++SP+ LKI+L+SIR+GRL  + +CL +E+R+  
Sbjct: 247 EIFSALEREVTQKPNDWLLATIQALEKASPSCLKISLRSIREGRLQGVGQCLIREYRMVC 306

Query: 313 NILRTLISGDVYEGIRALTIDKDNAPKWDPPSLEKVNDDRLNEVFQPYGEDLELQVPIDD 372
           ++++  IS D  EG RA+ IDKD  PKW P  LE V D  +++ F+           ++D
Sbjct: 307 HVMKGDISKDFVEGCRAVLIDKDRNPKWQPRRLEDVTDSMVDQYFE----------RVED 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHJ89.6e-15369.173-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Arabidopsis thaliana OX=3702... [more]
Q9LKJ13.5e-7842.233-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1... [more]
Q5XF591.5e-7643.183-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis t... [more]
Q6NMB08.1e-7541.16Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q1PEY51.2e-7341.21Probable 3-hydroxyisobutyryl-CoA hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KZ343.0e-218100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G608110 PE=3 SV=1[more]
A0A5A7UF779.5e-21295.873-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 OS=Cucumis melo var.... [more]
A0A1S3CNX29.5e-21295.873-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 OS=Cucumis melo OX=3... [more]
A0A1S3CNX66.8e-21095.363-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 OS=Cucumis melo OX=3... [more]
A0A6J1CL873.0e-18985.273-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Momordica charantia OX=3673 ... [more]
Match NameE-valueIdentityDescription
XP_004144631.16.3e-218100.003-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis sativus] >X... [more]
XP_031739896.15.2e-212100.003-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis sativus][more]
XP_008465449.12.0e-21195.87PREDICTED: 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X2 [Cucumis ... [more]
XP_008465447.11.4e-20995.36PREDICTED: 3-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Cucumis ... [more]
XP_038896721.15.5e-19891.213-hydroxyisobutyryl-CoA hydrolase-like protein 5 isoform X1 [Benincasa hispida] ... [more]
Match NameE-valueIdentityDescription
AT1G06550.16.8e-15469.17ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT5G65940.12.5e-7942.23beta-hydroxyisobutyryl-CoA hydrolase 1 [more]
AT3G60510.11.0e-7743.18ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT2G30660.15.7e-7641.16ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT2G30650.18.3e-7541.21ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR045004Enoyl-CoA hydratase/isomerase domainPFAMPF16113ECH_2coord: 22..353
e-value: 2.2E-107
score: 359.4
NoneNo IPR availableGENE3D3.90.226.10coord: 7..368
e-value: 2.8E-112
score: 377.3
NoneNo IPR availablePANTHERPTHR43176:SF23-HYDROXYISOBUTYRYL-COA HYDROLASE-LIKE PROTEIN 5coord: 7..385
NoneNo IPR availableCDDcd06558crotonase-likecoord: 22..203
e-value: 1.58005E-48
score: 161.189
IPR032259Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H typePANTHERPTHR431763-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATEDcoord: 7..385
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 9..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G21620.1CSPI04G21620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006574 valine catabolic process
molecular_function GO:0003860 3-hydroxyisobutyryl-CoA hydrolase activity