CSPI04G21140 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G21140
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGag/pol protein
LocationChr4: 19566804 .. 19567190 (+)
RNA-Seq ExpressionCSPI04G21140
SyntenyCSPI04G21140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTACACTAGGTCAGACATTTTTTATGTAGTGGGAATAATCAGTAGATATCAGTCCAACCTAGGGTTAGACCACTGGACGGTGGTTAAAATAATTCTCAAGTATCTTAGGAGAACAAGAGACTACATGTTTAGGTATGGAGTTAAAGATTTGATCCTTACAGGATACACTTACTCTGATTTCCAAACCGATAAGGATTCTAGGAAATCCACGTCAGGATCAGTGTTCATCCTAAACGGAGAGTTGTGGTATGACATAGCATCAAGCAAGGATGCATTGCAAATTCTACTATGGACGTTGAATATGTCGCTACTTGTGAAGCAGGAAAAGAACCAATTTGGCTCAGAAAGTTCCTACATGATTTGGAAGTTGTTCCAAACATGA

mRNA sequence

ATGCTCTACACTAGGTCAGACATTTTTTATGTAGTGGGAATAATCAGTAGATATCAGTCCAACCTAGGGTTAGACCACTGGACGGTGGTTAAAATAATTCTCAAGTATCTTAGGAGAACAAGAGACTACATGTTTAGGTATGGAGTTAAAGATTTGATCCTTACAGGATACACTTACTCTGATTTCCAAACCGATAAGGATTCTAGGAAATCCACGTCAGGATCAGTGTTCATCCTAAACGGAGAGTTGTGGTATGACATAGCATCAAGCAAGGATGCATTGCAAATTCTACTATGGACGTTGAATATGTCGCTACTTGTGAAGCAGGAAAAGAACCAATTTGGCTCAGAAAGTTCCTACATGATTTGGAAGTTGTTCCAAACATGA

Coding sequence (CDS)

ATGCTCTACACTAGGTCAGACATTTTTTATGTAGTGGGAATAATCAGTAGATATCAGTCCAACCTAGGGTTAGACCACTGGACGGTGGTTAAAATAATTCTCAAGTATCTTAGGAGAACAAGAGACTACATGTTTAGGTATGGAGTTAAAGATTTGATCCTTACAGGATACACTTACTCTGATTTCCAAACCGATAAGGATTCTAGGAAATCCACGTCAGGATCAGTGTTCATCCTAAACGGAGAGTTGTGGTATGACATAGCATCAAGCAAGGATGCATTGCAAATTCTACTATGGACGTTGAATATGTCGCTACTTGTGAAGCAGGAAAAGAACCAATTTGGCTCAGAAAGTTCCTACATGATTTGGAAGTTGTTCCAAACATGA

Protein sequence

MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYSDFQTDKDSRKSTSGSVFILNGELWYDIASSKDALQILLWTLNMSLLVKQEKNQFGSESSYMIWKLFQT*
Homology
[Close]
BLAST of CSPI04G21140 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-12
Identity = 37/81 (45.68%), Postives = 51/81 (62.96%), Query Frame = 0 

Query: 1    MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
            M+ TR DI + VG++SR+  N G +HW  VK IL+YLR T      +G  D IL GYT +
Sbjct: 1122 MVCTRPDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILKGYTDA 1181

Query: 61   DFQTDKDSRKSTSGSVFILNG 82
            D   D D+RKS++G +F  +G
Sbjct: 1182 DMAGDIDNRKSSTGYLFTFSG 1202

[Close]
BLAST of CSPI04G21140 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.8e-08
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 0 

Query: 1  MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDY---MFRYGVKDLILTGY 60
          M+ TR D+   VG++S++ S+    HW  +K +L+YL+ T+ Y     R G   L+  GY
Sbjct: 17 MVVTRPDLAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYGLEFTRAGTAKLV--GY 76

Query: 61 TYSDFQTDKDSRKSTSGSVFILNG 82
          + +D+  D +SR+STSG +F LNG
Sbjct: 77 SDADWAGDVESRRSTSGYLFKLNG 98

[Close]
BLAST of CSPI04G21140 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.2e-04
Identity = 27/80 (33.75%), Postives = 42/80 (52.50%), Query Frame = 0 

Query: 1    MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDY-MFRYGVKDLILTGYTY 60
            + +TR D+ Y V  +S+Y      DHW  +K +L+YL  T D+ +F      L L  Y+ 
Sbjct: 1238 LAFTRPDLSYAVNRLSQYMHMPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSD 1297

Query: 61   SDFQTDKDSRKSTSGSVFIL 80
            +D+  D D   ST+G +  L
Sbjct: 1298 ADWAGDTDDYVSTNGYIVYL 1317

[Close]
BLAST of CSPI04G21140 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 45.1 bits (105), Expect = 7.2e-04
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 0 

Query: 1    MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLI----LTG 60
            ML TR D+   V I+SRY S    + W  +K +L+YL+ T D    +  K+L     + G
Sbjct: 1192 MLCTRPDLTTAVNILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIF-KKNLAFENKIIG 1251

Query: 61   YTYSDFQTDKDSRKSTSGSVF 78
            Y  SD+   +  RKST+G +F
Sbjct: 1252 YVDSDWAGSEIDRKSTTGYLF 1271

[Close]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match: A0A5A7V8W0 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4427G00180 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 6.1e-30
Identity = 69/81 (85.19%), Postives = 71/81 (87.65%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI YVVGI+SRYQSN GLDHWT +KIILKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 816 MLCTRPDICYVVGIVSRYQSNPGLDHWTTIKIILKYLRRTRDYMLMYGAKDLILTGYTDS 875

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 876 DFQTDKDSRKSTSGSVFTLNG 896

[Close]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match: A0A5A7TKM4 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold246G00470 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.3e-29
Identity = 69/81 (85.19%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI Y VGI+SRYQSN GLDHWT VKIILKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 88  MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIILKYLRRTRDYMLVYGAKDLILTGYTDS 147

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 148 DFQTDKDSRKSTSGSVFTLNG 168

[Close]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match: A0A5A7TZD0 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00090 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-29
Identity = 68/81 (83.95%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1    MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
            ML TR DI Y VGI+SRYQSN GLDHWT VKI+LKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 1023 MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIVLKYLRRTRDYMLVYGAKDLILTGYTDS 1082

Query: 61   DFQTDKDSRKSTSGSVFILNG 82
            DFQTDKDSRKSTSGSVF LNG
Sbjct: 1083 DFQTDKDSRKSTSGSVFTLNG 1103

[Close]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match: A0A5A7UYE8 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001570 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-29
Identity = 68/81 (83.95%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI Y VGI+SRYQSN GLDHWT VKI+LKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 897 MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIVLKYLRRTRDYMLVYGAKDLILTGYTDS 956

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 957 DFQTDKDSRKSTSGSVFTLNG 977

[Close]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match: A0A5A7TJH9 (Putative Integrase core domain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold320G00110 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-29
Identity = 70/89 (78.65%), Postives = 73/89 (82.02%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           MLYTR DI Y VGI+SRYQSN GLDHWT VKIILKYLRRTRDYM  Y  KDLILTGYT S
Sbjct: 698 MLYTRPDICYAVGIVSRYQSNPGLDHWTAVKIILKYLRRTRDYMLVYEAKDLILTGYTDS 757

Query: 61  DFQTDKDSRKSTSGSVFILNGE--LWYDI 88
           DFQTDKD RKSTSGSVF LNG   +W+ I
Sbjct: 758 DFQTDKDHRKSTSGSVFTLNGGALVWHSI 786

[Close]
BLAST of CSPI04G21140 vs. NCBI nr
Match: KAA0062886.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-29
Identity = 69/81 (85.19%), Postives = 71/81 (87.65%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI YVVGI+SRYQSN GLDHWT +KIILKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 816 MLCTRPDICYVVGIVSRYQSNPGLDHWTTIKIILKYLRRTRDYMLMYGAKDLILTGYTDS 875

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 876 DFQTDKDSRKSTSGSVFTLNG 896

[Close]
BLAST of CSPI04G21140 vs. NCBI nr
Match: KAA0042496.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 138.3 bits (347), Expect = 4.8e-29
Identity = 69/81 (85.19%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI Y VGI+SRYQSN GLDHWT VKIILKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 88  MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIILKYLRRTRDYMLVYGAKDLILTGYTDS 147

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 148 DFQTDKDSRKSTSGSVFTLNG 168

[Close]
BLAST of CSPI04G21140 vs. NCBI nr
Match: KAA0025945.1 (gag/pol protein [Cucumis melo var. makuwa] >KAA0026303.1 gag/pol protein [Cucumis melo var. makuwa] >KAA0035786.1 gag/pol protein [Cucumis melo var. makuwa] >KAA0040492.1 gag/pol protein [Cucumis melo var. makuwa] >KAA0041262.1 gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 6.2e-29
Identity = 68/81 (83.95%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1    MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
            ML TR DI Y VGI+SRYQSN GLDHWT VKI+LKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 1023 MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIVLKYLRRTRDYMLVYGAKDLILTGYTDS 1082

Query: 61   DFQTDKDSRKSTSGSVFILNG 82
            DFQTDKDSRKSTSGSVF LNG
Sbjct: 1083 DFQTDKDSRKSTSGSVFTLNG 1103

[Close]
BLAST of CSPI04G21140 vs. NCBI nr
Match: KAA0043583.1 (putative Integrase core domain [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 6.2e-29
Identity = 70/89 (78.65%), Postives = 73/89 (82.02%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           MLYTR DI Y VGI+SRYQSN GLDHWT VKIILKYLRRTRDYM  Y  KDLILTGYT S
Sbjct: 698 MLYTRPDICYAVGIVSRYQSNPGLDHWTAVKIILKYLRRTRDYMLVYEAKDLILTGYTDS 757

Query: 61  DFQTDKDSRKSTSGSVFILNGE--LWYDI 88
           DFQTDKD RKSTSGSVF LNG   +W+ I
Sbjct: 758 DFQTDKDHRKSTSGSVFTLNGGALVWHSI 786

[Close]
BLAST of CSPI04G21140 vs. NCBI nr
Match: KAA0059226.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 6.2e-29
Identity = 68/81 (83.95%), Postives = 70/81 (86.42%), Query Frame = 0 

Query: 1   MLYTRSDIFYVVGIISRYQSNLGLDHWTVVKIILKYLRRTRDYMFRYGVKDLILTGYTYS 60
           ML TR DI Y VGI+SRYQSN GLDHWT VKI+LKYLRRTRDYM  YG KDLILTGYT S
Sbjct: 897 MLCTRPDICYAVGIVSRYQSNPGLDHWTAVKIVLKYLRRTRDYMLVYGAKDLILTGYTDS 956

Query: 61  DFQTDKDSRKSTSGSVFILNG 82
           DFQTDKDSRKSTSGSVF LNG
Sbjct: 957 DFQTDKDSRKSTSGSVFTLNG 977

The following BLAST results are available for this feature:
BLAST of CSPI04G21140 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P109782.5e-1245.68Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P0CV724.8e-0839.29Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P... [more]
Q9ZT943.2e-0433.75Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P041467.2e-0437.04Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
BLAST of CSPI04G21140 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7V8W06.1e-3085.19Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4427G001... [more]
A0A5A7TKM42.3e-2985.19Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold246G0047... [more]
A0A5A7TZD03.0e-2983.95Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G000... [more]
A0A5A7UYE83.0e-2983.95Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G0015... [more]
A0A5A7TJH93.0e-2978.65Putative Integrase core domain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
BLAST of CSPI04G21140 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0062886.11.3e-2985.19gag/pol protein [Cucumis melo var. makuwa][more]
KAA0042496.14.8e-2985.19gag/pol protein [Cucumis melo var. makuwa][more]
KAA0025945.16.2e-2983.95gag/pol protein [Cucumis melo var. makuwa] >KAA0026303.1 gag/pol protein [Cucumi... [more]
KAA0043583.16.2e-2978.65putative Integrase core domain [Cucumis melo var. makuwa][more]
KAA0059226.16.2e-2983.95gag/pol protein [Cucumis melo var. makuwa][more]
BLAST of CSPI04G21140 vs. NCBI nr
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G21140.1CSPI04G21140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding