CSPI04G20400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	17 kDa phloem lectin
Location	Chr4: 18518363 .. 18519016 (+)
RNA-Seq Expression	CSPI04G20400
Synteny	CSPI04G20400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGGCTACAGCACACATTATTTGGCATTTCCAAGAGCTTCCACAATAACATGGAGTGATGACACTCGATATTGGAGTTGGGCCACCGTAGATTTTTGCAGGTATAATCTTCAACTCAAATTTTATTTGTTCATTTATTCATATCTCATACATTTTCATCCATTTCTTTTGTCTTCATTCATAAAAATATAAACTAGTTATTAGATTCTACTTTCATGAATATAGGTTATGTTCCAATTATATGTTATTTATTATTGTTCTCTCTCACCATGGTATTTAAAACCTTGCAGCTACGCAATTGAAGAAGCCCGACTTTTACAAGTATCTTGGCTCGATTGTCGTTGGAGCATGGATGCATCTGATTTCAAACAAGATATTTGGTACAATGCAAGCGTTGAAGTAATGTTGACAAGCAACGCCTCTGGATGGAATGTTCCACTACACCTTGAAATCGAGTTGCCAGATGGGAGTAAGCAAGAGTCTCAAATAGTATTGGCAGGCAGACAACCAAATGTGTGGTTAAAGATTCCAATAGGTAAATTCATACTAAGGGGTTCTCTGACTAGCGGAACAATCCGATTCGGCTTCTACAACCATGAAGGGAATTGGAAAAGAGGCTTGAACATAAGAGCCCTTGCCATTCAAGCATAA

mRNA sequence

ATGGCAGGCTACAGCACACATTATTTGGCATTTCCAAGAGCTTCCACAATAACATGGAGTGATGACACTCGATATTGGAGTTGGGCCACCGTAGATTTTTGCAGCTACGCAATTGAAGAAGCCCGACTTTTACAAGTATCTTGGCTCGATTGTCGTTGGAGCATGGATGCATCTGATTTCAAACAAGATATTTGGTACAATGCAAGCGTTGAAGTAATGTTGACAAGCAACGCCTCTGGATGGAATGTTCCACTACACCTTGAAATCGAGTTGCCAGATGGGAGTAAGCAAGAGTCTCAAATAGTATTGGCAGGCAGACAACCAAATGTGTGGTTAAAGATTCCAATAGGTAAATTCATACTAAGGGGTTCTCTGACTAGCGGAACAATCCGATTCGGCTTCTACAACCATGAAGGGAATTGGAAAAGAGGCTTGAACATAAGAGCCCTTGCCATTCAAGCATAA

Coding sequence (CDS)

ATGGCAGGCTACAGCACACATTATTTGGCATTTCCAAGAGCTTCCACAATAACATGGAGTGATGACACTCGATATTGGAGTTGGGCCACCGTAGATTTTTGCAGCTACGCAATTGAAGAAGCCCGACTTTTACAAGTATCTTGGCTCGATTGTCGTTGGAGCATGGATGCATCTGATTTCAAACAAGATATTTGGTACAATGCAAGCGTTGAAGTAATGTTGACAAGCAACGCCTCTGGATGGAATGTTCCACTACACCTTGAAATCGAGTTGCCAGATGGGAGTAAGCAAGAGTCTCAAATAGTATTGGCAGGCAGACAACCAAATGTGTGGTTAAAGATTCCAATAGGTAAATTCATACTAAGGGGTTCTCTGACTAGCGGAACAATCCGATTCGGCTTCTACAACCATGAAGGGAATTGGAAAAGAGGCTTGAACATAAGAGCCCTTGCCATTCAAGCATAA

Protein sequence

MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDFKQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFILRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA*

Homology

[Close]

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot
Match: P0DSP5 (Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 6.1e-50
Identity = 81/150 (54.00%), Postives = 111/150 (74.00%), Query Frame = 0


Query: 5   STHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDFKQDI 64
           STH+L FPRA+T+TWSDDTRYWSW  VDFC Y +EEA+L +VSW DCRW+++ +D K ++
Sbjct: 8   STHFLLFPRAATLTWSDDTRYWSWNPVDFCGYQLEEAQLSRVSWFDCRWTVNTTDLKTNV 67

Query: 65  WYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFILRGS 124
           WYN  ++V + S ASGWN PL+LE+E+P+GSKQ SQ+VL  R  +VW K+ +G  ++  S
Sbjct: 68  WYNVFLKVQMGSGASGWNTPLNLELEMPNGSKQASQVVLNDRPRDVWFKLQMGNLMVSDS 127

Query: 125 LTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
            T G +R   YNH+ NWK G  +  LA++A
Sbjct: 128 ETCGALRMSLYNHQTNWKMGATLGPLALEA 157

[Close]

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot
Match: C0HJV2 (Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-25
Identity = 57/149 (38.26%), Postives = 84/149 (56.38%), Query Frame = 0


Query: 5   STHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDFKQDI 64
           S  ++ + R   ITWS D RYW W         +E A L+ V WL+   +++ S     I
Sbjct: 59  SNCFMLYARDLLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGI 118

Query: 65  WYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFILRGS 124
            Y A+ EVMLT++ASGW +P+ +++++PDGS+QESQ+ L  +   VW  I +G F +   
Sbjct: 119 SYEAAFEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISVGHFKISVG 178

Query: 125 LTSGTIRFGFYNHEGNWKRGLNIRALAIQ 154
            T G I F    H+   KRGL ++ L IQ
Sbjct: 179 ETIGNIEFSIVQHQ-EAKRGLLVKGLVIQ 206

[Close]

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot
Match: O81865 (Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana OX=3702 GN=PP2A1 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-18
Identity = 50/153 (32.68%), Postives = 81/153 (52.94%), Query Frame = 0


Query: 5   STHYLAFPRASTITWSDDTRYWSWAT-VDFCSYAIEEARLLQVSWLDCRWSMDASDFKQD 64
           S  ++ F +  +ITWSDD  YW+W T  +  +  +E   L  V WLD     D  +    
Sbjct: 92  SNCFMLFAKNLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPG 151

Query: 65  IWYNASVEVMLTSNASGWNVPLHLEIELPDGSK--QESQIVLAGRQPNVWLKIPIGKFIL 124
           I Y    +V L   A GW+ P++L++ LP+G +  QE ++ L       W+ + +G+F+ 
Sbjct: 152 IVYEVVFKVKLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVP 211

Query: 125 RGSLTSGTIRFGFYNH-EGNWKRGLNIRALAIQ 154
             S  +G I F  Y H  G WK+GL+++ +AI+
Sbjct: 212 EKS-AAGEITFSMYEHAAGVWKKGLSLKGVAIR 243

[Close]

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot
Match: Q9C8U9 (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702 GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.1e-18
Identity = 51/156 (32.69%), Postives = 77/156 (49.36%), Query Frame = 0


Query: 4   YSTHYLAFPRASTITWSDDTRYWSWATV--DFCSYAIEEARLLQ-VSWLDCRWSMDASDF 63
           Y   ++ + R  +I WSD   YWSW  +  D  S  + +A +L+ V WLD     D  + 
Sbjct: 8   YGQKHMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTREL 67

Query: 64  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSK--QESQIVLAGRQPNVWLKIPIGK 123
             +  Y     V L   ASGWN+P++L++ LPDG K  QE  + L       W+ I  G+
Sbjct: 68  TLETTYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGE 127

Query: 124 FILRGSLTSGTIRFGFYNHEG-NWKRGLNIRALAIQ 154
           F+      +G I F  Y  +   WKRGL ++ + I+
Sbjct: 128 FVTSPD-NAGEISFSMYETKSCCWKRGLFVKCVEIR 162

[Close]

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot
Match: Q9FHE8 (Protein PHLOEM PROTEIN 2-LIKE A6 OS=Arabidopsis thaliana OX=3702 GN=PP2A6 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.2e-10
Identity = 42/147 (28.57%), Postives = 73/147 (49.66%), Query Frame = 0


Query: 17  ITWSDDTRYWSWATVDFC--SYAIEEARLLQVSWLDCRWSMDASDFKQDIWYNASVEVML 76
           IT S+  + W+W+T++    S  IE A L +V WL    ++   +      Y A   V L
Sbjct: 246 ITHSEKPQKWTWSTINEAPNSAEIEIATLNKVYWLKIVGTITTENLTPGAKYEAVFVVKL 305

Query: 77  TSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNV-------WLKIPIGKFILRGSLTS 136
            +NASGW  P++L++++      + ++    R  N+       W+ I  G F++    T 
Sbjct: 306 ENNASGWEQPVNLKLKVVQHDGDDDRV---DRTENLNDYIGQNWVDILAGVFVVPPKTTP 365

Query: 137 GTIRFGFYNHEGNW-KRGLNIRALAIQ 154
            TI F  Y +E  + K+GL ++ +AI+
Sbjct: 366 ATIIFTMYQYEDKYKKKGLVVKGVAIR 389

[Close]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL
Match: Q8LK69 (17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17-7 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.0e-84
Identity = 150/154 (97.40%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           LRGSLTSGTIRFGFYNHEGNWKRGLNIR LAIQA
Sbjct: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRTLAIQA 154

[Close]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL
Match: A0A0A0KYP0 (Phloem lectin OS=Cucumis sativus OX=3659 GN=Csa_4G501830 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.3e-84
Identity = 150/154 (97.40%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           L GSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA
Sbjct: 121 LGGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL
Match: Q8LK70 (17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17-1 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.6e-83
Identity = 148/154 (96.10%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGS Q+SQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSTQKSQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           L GSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA
Sbjct: 121 LSGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL
Match: Q8L5A9 (17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.2e-78
Identity = 141/154 (91.56%), Postives = 146/154 (94.81%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMD SDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDVSDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           K+DIWYNASVEVMLTSNASGWNVPL+LEIELP GS+Q+SQIVL GRQPNVW KI +GKFI
Sbjct: 61  KKDIWYNASVEVMLTSNASGWNVPLYLEIELP-GSEQQSQIVLEGRQPNVWFKISLGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           L GSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA
Sbjct: 121 LSGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 153

[Close]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL
Match: A0A1S3CE66 (uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like OS=Cucumis melo OX=3656 GN=LOC103499442 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.8e-71
Identity = 120/154 (77.92%), Postives = 141/154 (91.56%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHY+A+PRA++ITWSDDTRYWSWA VDFC Y IEEARLLQVSWLDCRW+MD+S F
Sbjct: 1   MAGQSTHYVAYPRAASITWSDDTRYWSWAPVDFCGYPIEEARLLQVSWLDCRWTMDSSSF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQD+WYNASV+VM+T+ ASGWN+PL++EI++PDGSKQESQIVLAG+QPNVW KIP+GKFI
Sbjct: 61  KQDVWYNASVDVMMTNTASGWNIPLNIEIDMPDGSKQESQIVLAGKQPNVWFKIPLGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           +  S+TSG IRFGFYNH G+WKRGL +RAL IQA
Sbjct: 121 ISSSVTSGIIRFGFYNHGGHWKRGLIVRALLIQA 154

[Close]

BLAST of CSPI04G20400 vs. NCBI nr
Match: XP_031740320.1 (lectin-like [Cucumis sativus])

HSP 1 Score: 326.2 bits (835), Expect = 1.5e-85
Identity = 152/154 (98.70%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSW DCRWSMDASDF
Sbjct: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWFDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           LRGSLTSGTIRFG YNHEGNWKRGLNIRALAIQA
Sbjct: 121 LRGSLTSGTIRFGLYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. NCBI nr
Match: AAM77344.1 (17 kDa phloem lectin [Cucumis sativus])

HSP 1 Score: 322.4 bits (825), Expect = 2.1e-84
Identity = 150/154 (97.40%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           LRGSLTSGTIRFGFYNHEGNWKRGLNIR LAIQA
Sbjct: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRTLAIQA 154

[Close]

BLAST of CSPI04G20400 vs. NCBI nr
Match: KAE8649724.1 (hypothetical protein Csa_012573 [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 3.7e-84
Identity = 150/154 (97.40%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSW DCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWFDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           LRGSLTSGTIRFG YNHEGNWKRGLNIRALAIQA
Sbjct: 121 LRGSLTSGTIRFGLYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. NCBI nr
Match: XP_031740319.1 (lectin [Cucumis sativus])

HSP 1 Score: 321.2 bits (822), Expect = 4.8e-84
Identity = 150/154 (97.40%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           L GSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA
Sbjct: 121 LGGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. NCBI nr
Match: XP_031740318.1 (lectin-like [Cucumis sativus] >AAM77342.1 17 kDa phloem lectin [Cucumis sativus])

HSP 1 Score: 317.8 bits (813), Expect = 5.3e-83
Identity = 148/154 (96.10%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MAGYSTHYLAFPRASTITWSDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60
           MAG STHYLAFPRASTITW DDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF
Sbjct: 1   MAGQSTHYLAFPRASTITWGDDTRYWSWATVDFCSYAIEEARLLQVSWLDCRWSMDASDF 60

Query: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFI 120
           KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGS Q+SQIVLAGRQPNVW KIPIGKFI
Sbjct: 61  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSTQKSQIVLAGRQPNVWFKIPIGKFI 120

Query: 121 LRGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 155
           L GSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA
Sbjct: 121 LSGSLTSGTIRFGFYNHEGNWKRGLNIRALAIQA 154

[Close]

BLAST of CSPI04G20400 vs. TAIR 10
Match: AT4G19840.1 (phloem protein 2-A1 )

HSP 1 Score: 94.4 bits (233), Expect = 8.8e-20
Identity = 50/153 (32.68%), Postives = 81/153 (52.94%), Query Frame = 0


Query: 5   STHYLAFPRASTITWSDDTRYWSWAT-VDFCSYAIEEARLLQVSWLDCRWSMDASDFKQD 64
           S  ++ F +  +ITWSDD  YW+W T  +  +  +E   L  V WLD     D  +    
Sbjct: 92  SNCFMLFAKNLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPG 151

Query: 65  IWYNASVEVMLTSNASGWNVPLHLEIELPDGSK--QESQIVLAGRQPNVWLKIPIGKFIL 124
           I Y    +V L   A GW+ P++L++ LP+G +  QE ++ L       W+ + +G+F+ 
Sbjct: 152 IVYEVVFKVKLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVP 211

Query: 125 RGSLTSGTIRFGFYNH-EGNWKRGLNIRALAIQ 154
             S  +G I F  Y H  G WK+GL+++ +AI+
Sbjct: 212 EKS-AAGEITFSMYEHAAGVWKKGLSLKGVAIR 243

[Close]

BLAST of CSPI04G20400 vs. TAIR 10
Match: AT1G33920.1 (phloem protein 2-A4 )

HSP 1 Score: 91.7 bits (226), Expect = 5.7e-19
Identity = 51/156 (32.69%), Postives = 77/156 (49.36%), Query Frame = 0


Query: 4   YSTHYLAFPRASTITWSDDTRYWSWATV--DFCSYAIEEARLLQ-VSWLDCRWSMDASDF 63
           Y   ++ + R  +I WSD   YWSW  +  D  S  + +A +L+ V WLD     D  + 
Sbjct: 8   YGQKHMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTREL 67

Query: 64  KQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSK--QESQIVLAGRQPNVWLKIPIGK 123
             +  Y     V L   ASGWN+P++L++ LPDG K  QE  + L       W+ I  G+
Sbjct: 68  TLETTYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGE 127

Query: 124 FILRGSLTSGTIRFGFYNHEG-NWKRGLNIRALAIQ 154
           F+      +G I F  Y  +   WKRGL ++ + I+
Sbjct: 128 FVTSPD-NAGEISFSMYETKSCCWKRGLFVKCVEIR 162

[Close]

BLAST of CSPI04G20400 vs. TAIR 10
Match: AT5G45080.1 (phloem protein 2-A6 )

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-11
Identity = 42/147 (28.57%), Postives = 73/147 (49.66%), Query Frame = 0


Query: 17  ITWSDDTRYWSWATVDFC--SYAIEEARLLQVSWLDCRWSMDASDFKQDIWYNASVEVML 76
           IT S+  + W+W+T++    S  IE A L +V WL    ++   +      Y A   V L
Sbjct: 246 ITHSEKPQKWTWSTINEAPNSAEIEIATLNKVYWLKIVGTITTENLTPGAKYEAVFVVKL 305

Query: 77  TSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNV-------WLKIPIGKFILRGSLTS 136
            +NASGW  P++L++++      + ++    R  N+       W+ I  G F++    T 
Sbjct: 306 ENNASGWEQPVNLKLKVVQHDGDDDRV---DRTENLNDYIGQNWVDILAGVFVVPPKTTP 365

Query: 137 GTIRFGFYNHEGNW-KRGLNIRALAIQ 154
            TI F  Y +E  + K+GL ++ +AI+
Sbjct: 366 ATIIFTMYQYEDKYKKKGLVVKGVAIR 389

[Close]

BLAST of CSPI04G20400 vs. TAIR 10
Match: AT4G19850.1 (lectin-related )

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-10
Identity = 31/116 (26.72%), Postives = 59/116 (50.86%), Query Frame = 0


Query: 39  EEARLLQVSWLDCRWSMDASDFKQDIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQE 98
           E A++ +V+WL+     +      +  Y     V L  +A GW+  ++ ++ LP G  +E
Sbjct: 76  EVAKMERVAWLEVVGKFETEKLTPNSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGETKE 135

Query: 99  SQIVLAGRQPNVWLKIPIGKFILRGSLTSGTIRFGFYNHEGN-WKRGLNIRALAIQ 154
            +  +   + N W++IP G+F++     SG I F     + + WK GL ++ +AI+
Sbjct: 136 RRENVNLLERNKWVEIPAGEFMISPEHLSGKIEFSMLEVKSDQWKSGLIVKGVAIR 191

[Close]

BLAST of CSPI04G20400 vs. TAIR 10
Match: AT4G19850.2 (lectin-related )

HSP 1 Score: 62.4 bits (150), Expect = 3.7e-10
Identity = 35/128 (27.34%), Postives = 65/128 (50.78%), Query Frame = 0


Query: 8   YLAFPRASTITW--SDDTRYWSW-ATVDFCSYAI--EEARLLQVSWLDCRWSMDASDFKQ 67
           ++ + R  +ITW  S   +YWSW + +D  S  +  E A++ +V+WL+     +      
Sbjct: 65  FMLYARDLSITWAESQTNKYWSWFSDLDQTSSDVRTEVAKMERVAWLEVVGKFETEKLTP 124

Query: 68  DIWYNASVEVMLTSNASGWNVPLHLEIELPDGSKQESQIVLAGRQPNVWLKIPIGKFILR 127
           +  Y     V L  +A GW+  ++ ++ LP G  +E +  +   + N W++IP G+F++ 
Sbjct: 125 NSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGETKERRENVNLLERNKWVEIPAGEFMIS 184

Query: 128 GSLTSGTI 131
               SG I
Sbjct: 185 PEHLSGKI 192

The following BLAST results are available for this feature:

BLAST of CSPI04G20400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0DSP5	6.1e-50	54.00	Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1	[more]
C0HJV2	2.3e-25	38.26	Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1	[more]
O81865	1.2e-18	32.68	Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana OX=3702 GN=PP2A1 PE=2 S...	[more]
Q9C8U9	8.1e-18	32.69	Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FHE8	6.2e-10	28.57	Protein PHLOEM PROTEIN 2-LIKE A6 OS=Arabidopsis thaliana OX=3702 GN=PP2A6 PE=2 S...	[more]

BLAST of CSPI04G20400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q8LK69	1.0e-84	97.40	17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17-7 PE=4 SV=1	[more]
A0A0A0KYP0	2.3e-84	97.40	Phloem lectin OS=Cucumis sativus OX=3659 GN=Csa_4G501830 PE=4 SV=1	[more]
Q8LK70	2.6e-83	96.10	17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17-1 PE=4 SV=1	[more]
Q8L5A9	3.2e-78	91.56	17 kDa phloem lectin OS=Cucumis sativus OX=3659 GN=Lec17 PE=2 SV=1	[more]
A0A1S3CE66	3.8e-71	77.92	uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like OS=Cucumis melo OX=3656 GN...	[more]

BLAST of CSPI04G20400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740320.1	1.5e-85	98.70	lectin-like [Cucumis sativus]	[more]
AAM77344.1	2.1e-84	97.40	17 kDa phloem lectin [Cucumis sativus]	[more]
KAE8649724.1	3.7e-84	97.40	hypothetical protein Csa_012573 [Cucumis sativus]	[more]
XP_031740319.1	4.8e-84	97.40	lectin [Cucumis sativus]	[more]
XP_031740318.1	5.3e-83	96.10	lectin-like [Cucumis sativus] >AAM77342.1 17 kDa phloem lectin [Cucumis sativus]	[more]

BLAST of CSPI04G20400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19840.1	8.8e-20	32.68	phloem protein 2-A1	[more]
AT1G33920.1	5.7e-19	32.69	phloem protein 2-A4	[more]
AT5G45080.1	4.4e-11	28.57	phloem protein 2-A6	[more]
AT4G19850.1	2.8e-10	26.72	lectin-related	[more]
AT4G19850.2	3.7e-10	27.34	lectin-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025886	Phloem protein 2-like	PFAM	PF14299	PP2	coord: 12..153 e-value: 7.1E-21 score: 74.8
None	No IPR available	PANTHER	PTHR32278	FAMILY NOT NAMED	coord: 7..152
None	No IPR available	PANTHER	PTHR32278:SF42	PROTEIN PHLOEM PROTEIN 2-LIKE A2	coord: 7..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20400.1	CSPI04G20400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0030246	carbohydrate binding