CSPI04G18790 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G18790
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type domain-containing protein
Location	Chr4: 16316681 .. 16317412 (+)
RNA-Seq Expression	CSPI04G18790
Synteny	CSPI04G18790

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCAACCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTGTTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTTGGTGAAGATTCTTGA

mRNA sequence

ATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCAACCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTGTTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTTGGTGAAGATTCTTGA

Coding sequence (CDS)

ATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCAACCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTGTTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTTGGTGAAGATTCTTGA

Protein sequence

MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVEEQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQVNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFGEDS*

Homology

[Close]

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.4e-10
Identity = 30/86 (34.88%), Postives = 47/86 (54.65%), Query Frame = 0


Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           DH + P   K + + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 47  DHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEET---AIEMPCHHLFHSSCI 106

Query: 218 ENWFKNSHCCPICRFEMPISSDFGED 243
             W   ++ CP+CR+E+P   D  E+
Sbjct: 107 LPWLSKTNSCPLCRYELPTDDDTYEE 129

[Close]

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 0


Query: 183 ERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 239
           E  CVICL+E   ++  +   EMPC H FHG CIE W      CP+CR+EMP+  D
Sbjct: 110 EGECVICLEEWKSEETVK---EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGD 162

[Close]

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = 0


Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           DH + P   K   + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 47  DHHLPPPAAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEET---AIEMPCHHLFHSNCI 106

Query: 218 ENWFKNSHCCPICRFEMPISSDFGED 243
             W   ++ CP+CR E+P   D  E+
Sbjct: 107 LPWLSKTNSCPLCRHELPTDDDTYEE 129

[Close]

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 30/86 (34.88%), Postives = 47/86 (54.65%), Query Frame = 0


Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           +H + P   KA+ + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 59  EHHLPPPAAKAVVESLPRTVIRSSKAELKCPVCLLEFEEEET---VIEMPCHHLFHSNCI 118

Query: 218 ENWFKNSHCCPICRFEMPISSDFGED 243
             W   ++ CP+CR E+P   D  E+
Sbjct: 119 LPWLSKTNSCPLCRHELPTDDDSYEE 141

[Close]

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.8e-09
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 0


Query: 186 CVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFGE 242
           CVIC +E+ +   GR   EMPC H FH KCI  W    + CP CRF++P    F E
Sbjct: 214 CVICKEEMSE---GRDVCEMPCQHFFHWKCILPWLSKKNTCPFCRFQLPTDDVFSE 266

[Close]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL
Match: A0A0A0L236 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 9.7e-138
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQISRYSTTAVEVDIDVPKWTKIPK PSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFG 240
           EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD  
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240

Query: 241 EDS 244
           EDS
Sbjct: 241 EDS 243

[Close]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.4e-120
Identity = 208/242 (85.95%), Postives = 224/242 (92.56%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I K PSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFG 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS FG
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 243
           ED
Sbjct: 241 ED 242

[Close]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.4e-120
Identity = 208/242 (85.95%), Postives = 224/242 (92.56%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I K PSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFG 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS FG
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 243
           ED
Sbjct: 241 ED 242

[Close]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL
Match: A0A6J1GK53 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3662 GN=LOC111455016 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 4.0e-75
Identity = 143/237 (60.34%), Postives = 181/237 (76.37%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPK PS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 236
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL
Match: A0A6J1I8N9 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=3661 GN=LOC111471014 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.2e-73
Identity = 141/240 (58.75%), Postives = 180/240 (75.00%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPK PS IE IGL+FTLR R + F+V E+GER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L  SF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RLE  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 239
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI  +
Sbjct: 181 EVDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQKN 237

[Close]

BLAST of CSPI04G18790 vs. NCBI nr
Match: XP_011654386.1 (RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_012982 [Cucumis sativus])

HSP 1 Score: 499.2 bits (1284), Expect = 2.0e-137
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQISRYSTTAVEVDIDVPKWTKIPK PSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFG 240
           EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD  
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240

Query: 241 EDS 244
           EDS
Sbjct: 241 EDS 243

[Close]

BLAST of CSPI04G18790 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger protein 141-like [Cucumis melo var. makuwa] >TYK09674.1 RING finger protein 141-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 441.4 bits (1134), Expect = 5.0e-120
Identity = 208/242 (85.95%), Postives = 224/242 (92.56%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I K PSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFG 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS FG
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 243
           ED
Sbjct: 241 ED 242

[Close]

BLAST of CSPI04G18790 vs. NCBI nr
Match: KAG6571896.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.5 bits (750), Expect = 1.7e-75
Identity = 145/237 (61.18%), Postives = 181/237 (76.37%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPK PS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLTI  +V +Y +G  GS  GG+   +VPAC  A+ KML+RLE  +
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGV-GS--GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 236
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of CSPI04G18790 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7011584.1 HRD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 291.2 bits (744), Expect = 8.3e-75
Identity = 143/237 (60.34%), Postives = 181/237 (76.37%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPK PS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 236
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of CSPI04G18790 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 289.7 bits (740), Expect = 2.4e-74
Identity = 143/237 (60.34%), Postives = 181/237 (76.37%), Query Frame = 0


Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKQPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY +  VEV  D+P  ++IPK PS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYESLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLTI  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 236
           E + +CVICLDE+   KKKNGR  L+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

[Close]

BLAST of CSPI04G18790 vs. TAIR 10
Match: AT1G21960.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 7.4e-13
Identity = 45/158 (28.48%), Postives = 74/158 (46.84%), Query Frame = 0


Query: 87  FQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQVNSRSRDKVFSMTVNLTITDVVLQY- 146
           ++ +  S+ N   K++  + ++ L EQ+++I N   +    K  SM  +     +V  Y 
Sbjct: 49  YEGVISSYPNDSKKLNDWLYSAGLSEQYVDIANSQLTERICKSISMVDSSVCATLVYMYC 108

Query: 147 --VVGEDGSTFGGEDHRVV---PA----CDKALKKMLKRLEMREEDERVCVICLDEVGKK 206
             +V     +F    + +    PA     D+  +K+  + +    D  VC ICL+E  K 
Sbjct: 109 KVIVPPRIDSFDETTNNIELIRPANKLVVDRLARKVYNKKKKSNSDASVCTICLEEFEK- 168

Query: 207 KNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMP 235
             G I + +PC H F   CI  WF   H CP+CRFE+P
Sbjct: 169 --GEIVVTLPCGHEFDDGCIGKWFLKDHVCPLCRFELP 203

[Close]

BLAST of CSPI04G18790 vs. TAIR 10
Match: AT3G15740.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-12
Identity = 39/100 (39.00%), Postives = 52/100 (52.00%), Query Frame = 0


Query: 143 LQYVVGEDGSTFGGEDHR--------VVPACDKALKKMLKRLEMREEDERVCVICLDEVG 202
           +Q  +G   S F GED          V PA D+A++  L+ L +  E+E  CVIC+D + 
Sbjct: 127 VQVKIGVTVSRFPGEDDHVDVQVQLAVAPANDEAVEMHLETLVV--ENEGYCVICMDNIR 186

Query: 203 KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMP 235
              +   G  MPC HVFH  C E W +NS  CP+CR   P
Sbjct: 187 VGSDVEAG-RMPCSHVFHRTCGEEWLRNSGICPVCRALFP 223

[Close]

BLAST of CSPI04G18790 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-12
Identity = 36/90 (40.00%), Postives = 50/90 (55.56%), Query Frame = 0


Query: 149 EDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERV---CVICLDEVGKKKNGRIGLEM 208
           +D  + GG+  R  PA    ++ M  R+ + E+ E+    C ICLDE  K   G +  EM
Sbjct: 71  DDFFSDGGKQGR-SPALKSEVENM-PRVVIGEDKEKYGGSCAICLDEWSK---GDVAAEM 130

Query: 209 PCLHVFHGKCIENWFKNSHCCPICRFEMPI 236
           PC H FH KC+E W      CP+CR+EMP+
Sbjct: 131 PCKHKFHSKCVEEWLGRHATCPMCRYEMPV 155

[Close]

BLAST of CSPI04G18790 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-10
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 0


Query: 183 ERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 239
           E  CVICL+E   ++  +   EMPC H FHG CIE W      CP+CR+EMP+  D
Sbjct: 110 EGECVICLEEWKSEETVK---EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGD 162

[Close]

BLAST of CSPI04G18790 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 2.0e-10
Identity = 34/104 (32.69%), Postives = 54/104 (51.92%), Query Frame = 0


Query: 140 DVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERVCVICLDEVGKKKNG 199
           D++LQ++   D +  G       P   K   + L  +++ E  +  C +CLD+  K   G
Sbjct: 221 DLLLQHLAENDPNRQG------TPPARKEAVEALPTVKIMEPLQ--CSVCLDDFEK---G 280

Query: 200 RIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDFGEDS 244
               EMPC H FH +CI  W +    CP+CRFE+P S+D  +++
Sbjct: 281 TEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPSSADDDDET 313

The following BLAST results are available for this feature:

BLAST of CSPI04G18790 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9P0P0	4.4e-10	34.88	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]
Q9LQX2	2.2e-09	48.21	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q3T0W3	2.2e-09	34.88	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q6AXU4	2.8e-09	34.88	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
Q8GXF8	4.8e-09	46.43	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of CSPI04G18790 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L236	9.7e-138	98.77	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 P...	[more]
A0A5A7SNF1	2.4e-120	85.95	RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S4E0Q9	2.4e-120	85.95	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]
A0A6J1GK53	4.0e-75	60.34	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1I8N9	2.2e-73	58.75	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=366...	[more]

BLAST of CSPI04G18790 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654386.1	2.0e-137	98.77	RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_0...	[more]
XP_016901804.1	5.0e-120	85.95	PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger...	[more]
KAG6571896.1	1.7e-75	61.18	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022952293.1	8.3e-75	60.34	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7...	[more]
XP_023511497.1	2.4e-74	60.34	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]

BLAST of CSPI04G18790 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21960.1	7.4e-13	28.48	RING/U-box superfamily protein	[more]
AT3G15740.1	2.2e-12	39.00	RING/U-box superfamily protein	[more]
AT1G14200.1	2.8e-12	40.00	RING/U-box superfamily protein	[more]
AT1G26800.1	1.5e-10	48.21	RING/U-box superfamily protein	[more]
AT5G56340.1	2.0e-10	32.69	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 185..230 e-value: 0.0042 score: 10.4
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 186..229 e-value: 1.1E-5 score: 34.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 186..230 e-value: 6.4E-11 score: 42.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 186..230 score: 12.046688
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 162..237 e-value: 5.2E-16 score: 59.6
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 104..234
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 104..234
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G18790.1	CSPI04G18790.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding