CSPI04G17960 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17960
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr4: 15407191 .. 15408899 (-)
RNA-Seq ExpressionCSPI04G17960
SyntenyCSPI04G17960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATCCATAAAGCCATTCCTTTCTCCACTCCCTCTCCCATCCTTTTTCCCCATTATATAAATTCTTCCCACATTCTGATTCCCTTTCCCAAATCATTTCTCTAACTAACAAAACTAATCACCAACTTCCACCATTTCCAAACATTTGTTCGTCTTTCCCATGTCTTTGACCCCACCCCAATCTACAACCAACAACCGCCATACCACAACCTTCCAGCTCTACTGGTGCCACCAATGCCACCGCACCGTTACCCTCGCCTCAGCACACCCTTCGGAGCTCACCTGCCCACGCTGTTTTGGGCAGTTCATTGAAGAGGTGCAACTCACACTCCCTGAATTTAATCCCTCGCCAGAGGGTCGTTTATTTGAAGCCCTCTCACTAATGCTCAACCAGCCGATTCGTCTCTTCAATAACAGAACCCCAAATGGGAACCGCCACCATCCACCATGGCACAGGTTCGAGGAATTTGATCGGAGATCGTTTAGTGATCCTGAAGGAGATGAATTGCCTCAATGGCGAAGACGATGGCGTAGTCGAAGTCTGGACGAGAGGGACAACTTTGGCCAACAACCTCCAAATCCAAATCGGTCTCGTACTGTTATCGTTTTTGGTCCCCCTGACCAACTTCAGCCAATCCAGCCGATAATACCACGGAGGATCAGTCCAAGAGACTACTTCACTGGACCACAACTGGATGAGTTAATTGAAGAGCTGACGCAGAACGACCGACCAGGACCGGCACCGGCAAGCGAGGAGGCAATCGAGAGAATTCCAACGGTGAAAATAGAAGCAGAGCATCTGAAGAACGAGTCGCACTGCCCAGTTTGTAAGGAGGAGTTTGAAGTGGGGGGAGAAGCAAGGGAGTTAAGTTGTAAGCATATATACCATTCGGAGTGCATAGTACCATGGCTTCGGCTTCATAACAGTTGCCCGGTTTGTAGGCAAGAGATGCCGTCATTTACACCGGAGAATGAAGCGTCGAGTAGTTCTGAAGAGGAAGGGATGGGGAGAAGATGTGCCAGATGGTGGAGTCACCTGGTGCCTTGGCCATTTCGGAATAGGTATAGGCAGATTAGTCCATTTCCACGGAACCGCTTCGATGCTTCTTCTCGAGGTGATGCATAATTTGTTCTTTCTACTCTTCAAAATAATGGAATGTCTCCAGAATAGGGATATTGATATACGAATAAATAAAATTTGTATGACCATAACTAAAGATGCACATACAATTAATTAGGGATCTATTGCTGATGATCTTACATTTGTTACGTCTCTAATGGGAGATAAAATCATGCCTAAAATTATTTTCCTCTTCATCCCATTATTCTAAATCTTCGGCCAAAGAAATCAATTCGCATACTTTCTACGGGCGGAGAAACAATTTTACGAATTAAAAAATCATTTAAAAAGCTAATGCATAAAATTTAAAACTAATAAATAAATAAGTATATATATACATATTTAGAAGTTTCACGAATTCTACTAAATTGAAAATAAAAGCATAAAAAGTAAACAAATAAAAACAGTTCTATAATTCCATCACGGATTCTAACCCAGATATTTTATTTCATTTCCCAGAAATTAATTCGAGGGGTGGTTCATCCTACCTCTCTTCCTCAGCCCTTCTAATGTCGATGTTTATGCTGCAATTAACTTGGATTATACTTGAAACTTTTCGCTAGGAATGTAAAATACATGTGGGCGTGTA

mRNA sequence

CATATCCATAAAGCCATTCCTTTCTCCACTCCCTCTCCCATCCTTTTTCCCCATTATATAAATTCTTCCCACATTCTGATTCCCTTTCCCAAATCATTTCTCTAACTAACAAAACTAATCACCAACTTCCACCATTTCCAAACATTTGTTCGTCTTTCCCATGTCTTTGACCCCACCCCAATCTACAACCAACAACCGCCATACCACAACCTTCCAGCTCTACTGGTGCCACCAATGCCACCGCACCGTTACCCTCGCCTCAGCACACCCTTCGGAGCTCACCTGCCCACGCTGTTTTGGGCAGTTCATTGAAGAGGTGCAACTCACACTCCCTGAATTTAATCCCTCGCCAGAGGGTCGTTTATTTGAAGCCCTCTCACTAATGCTCAACCAGCCGATTCGTCTCTTCAATAACAGAACCCCAAATGGGAACCGCCACCATCCACCATGGCACAGGTTCGAGGAATTTGATCGGAGATCGTTTAGTGATCCTGAAGGAGATGAATTGCCTCAATGGCGAAGACGATGGCGTAGTCGAAGTCTGGACGAGAGGGACAACTTTGGCCAACAACCTCCAAATCCAAATCGGTCTCGTACTGTTATCGTTTTTGGTCCCCCTGACCAACTTCAGCCAATCCAGCCGATAATACCACGGAGGATCAGTCCAAGAGACTACTTCACTGGACCACAACTGGATGAGTTAATTGAAGAGCTGACGCAGAACGACCGACCAGGACCGGCACCGGCAAGCGAGGAGGCAATCGAGAGAATTCCAACGGTGAAAATAGAAGCAGAGCATCTGAAGAACGAGTCGCACTGCCCAGTTTGTAAGGAGGAGTTTGAAGTGGGGGGAGAAGCAAGGGAGTTAAGTTGTAAGCATATATACCATTCGGAGTGCATAGTACCATGGCTTCGGCTTCATAACAGTTGCCCGGTTTGTAGGCAAGAGATGCCGTCATTTACACCGGAGAATGAAGCGTCGAGTAGTTCTGAAGAGGAAGGGATGGGGAGAAGATGTGCCAGATGGTGGAGTCACCTGGTGCCTTGGCCATTTCGGAATAGGTATAGGCAGATTAGTCCATTTCCACGGAACCGCTTCGATGCTTCTTCTCGAGAAATTAATTCGAGGGGTGGTTCATCCTACCTCTCTTCCTCAGCCCTTCTAATGTCGATGTTTATGCTGCAATTAACTTGGATTATACTTGAAACTTTTCGCTAGGAATGTAAAATACATGTGGGCGTGTA

Coding sequence (CDS)

ATGTCTTTGACCCCACCCCAATCTACAACCAACAACCGCCATACCACAACCTTCCAGCTCTACTGGTGCCACCAATGCCACCGCACCGTTACCCTCGCCTCAGCACACCCTTCGGAGCTCACCTGCCCACGCTGTTTTGGGCAGTTCATTGAAGAGGTGCAACTCACACTCCCTGAATTTAATCCCTCGCCAGAGGGTCGTTTATTTGAAGCCCTCTCACTAATGCTCAACCAGCCGATTCGTCTCTTCAATAACAGAACCCCAAATGGGAACCGCCACCATCCACCATGGCACAGGTTCGAGGAATTTGATCGGAGATCGTTTAGTGATCCTGAAGGAGATGAATTGCCTCAATGGCGAAGACGATGGCGTAGTCGAAGTCTGGACGAGAGGGACAACTTTGGCCAACAACCTCCAAATCCAAATCGGTCTCGTACTGTTATCGTTTTTGGTCCCCCTGACCAACTTCAGCCAATCCAGCCGATAATACCACGGAGGATCAGTCCAAGAGACTACTTCACTGGACCACAACTGGATGAGTTAATTGAAGAGCTGACGCAGAACGACCGACCAGGACCGGCACCGGCAAGCGAGGAGGCAATCGAGAGAATTCCAACGGTGAAAATAGAAGCAGAGCATCTGAAGAACGAGTCGCACTGCCCAGTTTGTAAGGAGGAGTTTGAAGTGGGGGGAGAAGCAAGGGAGTTAAGTTGTAAGCATATATACCATTCGGAGTGCATAGTACCATGGCTTCGGCTTCATAACAGTTGCCCGGTTTGTAGGCAAGAGATGCCGTCATTTACACCGGAGAATGAAGCGTCGAGTAGTTCTGAAGAGGAAGGGATGGGGAGAAGATGTGCCAGATGGTGGAGTCACCTGGTGCCTTGGCCATTTCGGAATAGGTATAGGCAGATTAGTCCATTTCCACGGAACCGCTTCGATGCTTCTTCTCGAGAAATTAATTCGAGGGGTGGTTCATCCTACCTCTCTTCCTCAGCCCTTCTAATGTCGATGTTTATGCTGCAATTAACTTGGATTATACTTGAAACTTTTCGCTAG

Protein sequence

MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEFNPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRNRYRQISPFPRNRFDASSREINSRGGSSYLSSSALLMSMFMLQLTWIILETFR*
Homology
BLAST of CSPI04G17960 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.6e-34
Identity = 108/315 (34.29%), Postives = 149/315 (47.30%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQL----TLPEFNPSPEGRLFEALSLML 80
           +WCH+C R V L +    +  C  C G F+EE+ +       +    P   L EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 81  NQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQ 140
                   +R    +       R       SFS                 +L     FG 
Sbjct: 68  -------RSRLAERSYDREISGRLGSAGSESFS-----------------NLAPLLIFGG 127

Query: 141 QPP-----NPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQ-NDR 200
           Q P       N S    V G    +      I R  +  DYF GP L+ELIE+L+     
Sbjct: 128 QAPFRLAGGDNSSVEAFVNGAAPGIG-----IARGTNAGDYFFGPGLEELIEQLSSGTHH 187

Query: 201 PGPAPASEEAIERIPTVKIEAEHLK-NESHCPVCKEEFEVGGEARELSCKHIYHSECIVP 260
            GP PA + +I+ +PT+KI  +HLK ++SHCPVCK+EFE+  EA+++ C HIYHS+CIVP
Sbjct: 188 RGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVP 247

Query: 261 WLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCAR---WWSHLVPWPFRNRYRQIS 320
           WL  HNSCPVCR+E+PS    +   SS      GR  +R    +S+L  WPFR+     S
Sbjct: 248 WLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNL--WPFRS---SSS 285

Query: 321 PFPRNRFDASSRGDA 322
              +NR D ++   A
Sbjct: 308 SSTQNRRDTNNTATA 285

BLAST of CSPI04G17960 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-33
Identity = 104/310 (33.55%), Postives = 150/310 (48.39%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHP-SELTCPRCFGQFIEEVQLTLPE-----FNPSPEGRLFEALSL 80
           ++C+QC+RTV +  + P S+ TCP C   F+EE     P       NP+P    F  L L
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 81  MLNQPI-RLFNNRTPNGNRHHPPWHRFEEFDRRSFSDP----EGDELPQWRRRWRSRSLD 140
            L+ P   L     P+ +          + +  S S P     GD        +    L+
Sbjct: 83  TLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLN 142

Query: 141 ERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQND 200
           +  + G Q         VI   P DQ       +P  I   DYF GP L++LI++L +ND
Sbjct: 143 DLRSSGAQ------IEFVIQNNPSDQ----GFRLPANIG--DYFIGPGLEQLIQQLAEND 202

Query: 201 --RPGPAPASEEAIERIPTVKIEAEHLKNE-SHCPVCKEEFEVGGEARELSCKHIYHSEC 260
             R G  PAS+ AIE +P V I   +L +E + C VC ++FE G EA+++ CKH+YH +C
Sbjct: 203 PNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDC 262

Query: 261 IVPWLRLHNSCPVCRQEMPSFTPENEA---------------SSSSEEEGMGRRCARWWS 302
           ++PWL LHNSCPVCR E+P+  P+ E                +S    +G  R   R + 
Sbjct: 263 LLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFR 320

BLAST of CSPI04G17960 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.6e-31
Identity = 97/302 (32.12%), Postives = 151/302 (50.00%), Query Frame = 0

Query: 19  QLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLP----EFNPSPEGRLFEALSL 78
           ++++C+QC++TVT++ +  ++  CP C   F+EE +   P     FNP+     F     
Sbjct: 19  KMFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADP 78

Query: 79  MLNQPIRLFNN--RTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERD 138
                  +F +    P+G             D  S   P     PQ R   ++   D  D
Sbjct: 79  FSTLLPLIFGSSAAAPSG------------MDFMSLFGPSMQ--PQARSTQQNPQSDAFD 138

Query: 139 NFGQQPPNPNRSRTVIVFGPPDQL----QPIQPIIPRRISPRDYFTGPQLDELIEELTQN 198
            F       N  +T+   G   +      P  P      +  DYF GP L++LI++L +N
Sbjct: 139 PFTFL---QNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEN 198

Query: 199 D--RPGPAPASEEAIERIPTVKIEAEHLKNE-SHCPVCKEEFEVGGEARELSCKHIYHSE 258
           D  R G  PAS+ AI+ +PTVK+  + LK+E + C VC +EFE G + +++ CKH++H +
Sbjct: 199 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 258

Query: 259 CIVPWLRLHNSCPVCRQEMPSFTPENE-ASSSSEEEGMGRRCARW-----WSHLVPWPFR 302
           C++PWL LHNSCPVCR E+P+  P+ E  S  S+  G G+          +S  +PWPFR
Sbjct: 259 CLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQLPWPFR 303

BLAST of CSPI04G17960 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-31
Identity = 110/306 (35.95%), Postives = 143/306 (46.73%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLP---EFNPSPEGRLFEALSLMLN 80
           +WCH+C R V L   H  E  C  C G F+EE+ +      +   S  G +    +  L 
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 81  QPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQQ 140
               +F       NR     H  E   R   S PE               L     FG Q
Sbjct: 68  DAFSVFMR-----NRLAERSHDREIRGRTISSGPE-----------NFPGLAPLLIFGGQ 127

Query: 141 PP----NPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQ-NDRPG 200
            P      N    +   G P        I   R +  DYF GP L+EL E+L+    R G
Sbjct: 128 VPYRLTGDNAVEALFNGGSPG-------IGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 187

Query: 201 PAPASEEAIERIPTVKIEAEHLK-NESHCPVCKEEFEVGGEARELSCKHIYHSECIVPWL 260
           P PA   AI+ +PT+KI   HL+ ++S+CPVCK+EFE+G EA+++ C HIYHS+CIVPWL
Sbjct: 188 PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWL 247

Query: 261 RLHNSCPVCRQEMPS----------FTP-------ENEASSSSEEEGMGRRCARWWSHLV 301
             HNSCPVCRQE+PS           TP        + +SS+S E G  RR    +S   
Sbjct: 248 VQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERR--NPFSSF- 283

BLAST of CSPI04G17960 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.1e-24
Identity = 93/303 (30.69%), Postives = 138/303 (45.54%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTL----ASAHPSELTCPRCFGQFIEEV--- 60
           M  TP   TT    T +   YWC+ C R V++     +     + CP C G FIE++   
Sbjct: 1   MPSTPTTPTTTTPITAS---YWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDS 60

Query: 61  -----QLTLPEFNPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSF 120
                +LT+P    S E R        +N   R    R  +G R  P ++        + 
Sbjct: 61  SSAATELTIP---ASTEVR-------SINNNRRSVIRRRRSGRR--PSFNPVIVLQGGAG 120

Query: 121 SDPEGDELPQWR-RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRI 180
              EG+E    R RR      D+    G +P                        +P  +
Sbjct: 121 EREEGEEGDAARDRRAFEFYYDDGSGSGLRP------------------------LPDSV 180

Query: 181 SPRDYFTGPQLDELIEELTQND-------RPGPAPASEEAIERIPTVKIEAEHLKNESHC 240
           S  +   G   + L+E+L+Q +       R G  PAS+ AIE +P V+I   H+ +E++C
Sbjct: 181 S--EILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANC 240

Query: 241 PVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEE 284
            VC E FE   EARE+ CKH++H +CIVPWL + NSCPVCR E+PS  P   ++++ E+ 
Sbjct: 241 AVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPS-EPNRRSNNNEEDN 261

BLAST of CSPI04G17960 vs. ExPASy TrEMBL
Match: A0A0A0L0L2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE=4 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.2e-191
Identity = 316/318 (99.37%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NPSPEGRLFEALSLMLNQPIR+FNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPSPEGRLFEALSLMLNQPIRIFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPI+PRRISPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPILPRRISPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSR 319
           RYRQISPFPRNRFDASSR
Sbjct: 301 RYRQISPFPRNRFDASSR 318

BLAST of CSPI04G17960 vs. ExPASy TrEMBL
Match: A0A5A7TUM6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00540 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.4e-184
Identity = 305/321 (95.02%), Postives = 311/321 (96.88%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSRGDA 322
           RYRQISPFPRNRFDASSRGDA
Sbjct: 301 RYRQISPFPRNRFDASSRGDA 321

BLAST of CSPI04G17960 vs. ExPASy TrEMBL
Match: A0A1S3B9M6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.5e-183
Identity = 303/319 (94.98%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSRG 320
           RYRQISPFPRNRFDASSRG
Sbjct: 301 RYRQISPFPRNRFDASSRG 319

BLAST of CSPI04G17960 vs. ExPASy TrEMBL
Match: A0A1S4DVY7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.7e-182
Identity = 302/318 (94.97%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSR 319
           RYRQISPFPRNRFDASSR
Sbjct: 301 RYRQISPFPRNRFDASSR 318

BLAST of CSPI04G17960 vs. ExPASy TrEMBL
Match: A0A6J1IKH7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.3e-142
Identity = 248/320 (77.50%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 1   MSLTPPQSTTNN-RHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPE 60
           M+L+PP  TTN+ RH  TF LYWCHQCHRTVTLASA PS+LTCPRCFG F+EEV L LPE
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLPE 60

Query: 61  FNPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQW 120
            +PSPE RL EALSLML+ P+RLFN R P+ +R  PP HRF++FDRRSFSDPEGDE   W
Sbjct: 61  LDPSPETRLLEALSLMLDPPVRLFNYRHPDPDRRRPPLHRFDDFDRRSFSDPEGDEWLHW 120

Query: 121 RRRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLD 180
           R R RSRSLDERDN G+QPPNPN+SRTVIV GPPDQ QPIQPIIPRR +PRDYFTGP+LD
Sbjct: 121 RTRRRSRSLDERDNSGRQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYFTGPRLD 180

Query: 181 ELIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCK 240
           ELIE+LTQNDRPGP PA EEAIERIPTVKI AEHLKN+SHCPVCKEEFEVGGEARELSCK
Sbjct: 181 ELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCK 240

Query: 241 HIYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEE-GMGRRCARWWSHLVPWPF 300
           HIYHSECIVPWLRLHNSCPVCRQE+P   PEN A+ +SE E  +GR C RWW+ LV W F
Sbjct: 241 HIYHSECIVPWLRLHNSCPVCRQEIPPLPPENGAAGNSETEVRIGRACTRWWNRLVSWLF 300

Query: 301 RNRYRQISP-FPRNRFDASS 318
           RNRYRQISP  P NRF+ASS
Sbjct: 301 RNRYRQISPSSPPNRFNASS 320

BLAST of CSPI04G17960 vs. NCBI nr
Match: XP_004142693.2 (E3 ubiquitin-protein ligase RZF1 [Cucumis sativus])

HSP 1 Score: 681.0 bits (1756), Expect = 4.9e-192
Identity = 317/319 (99.37%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NPSPEGRLFEALSLMLNQPIR+FNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPSPEGRLFEALSLMLNQPIRIFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPI+PRRISPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPILPRRISPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSRG 320
           RYRQISPFPRNRFDASSRG
Sbjct: 301 RYRQISPFPRNRFDASSRG 319

BLAST of CSPI04G17960 vs. NCBI nr
Match: KGN54579.1 (hypothetical protein Csa_012041 [Cucumis sativus])

HSP 1 Score: 678.7 bits (1750), Expect = 2.4e-191
Identity = 316/318 (99.37%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NPSPEGRLFEALSLMLNQPIR+FNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPSPEGRLFEALSLMLNQPIRIFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPI+PRRISPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPILPRRISPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSR 319
           RYRQISPFPRNRFDASSR
Sbjct: 301 RYRQISPFPRNRFDASSR 318

BLAST of CSPI04G17960 vs. NCBI nr
Match: KAA0045221.1 (E3 ubiquitin-protein ligase RING1 isoform X2 [Cucumis melo var. makuwa] >TYK19249.1 E3 ubiquitin-protein ligase RING1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 654.4 bits (1687), Expect = 4.9e-184
Identity = 305/321 (95.02%), Postives = 311/321 (96.88%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSRGDA 322
           RYRQISPFPRNRFDASSRGDA
Sbjct: 301 RYRQISPFPRNRFDASSRGDA 321

BLAST of CSPI04G17960 vs. NCBI nr
Match: XP_008444136.1 (PREDICTED: E3 ubiquitin-protein ligase RING1 isoform X2 [Cucumis melo])

HSP 1 Score: 650.6 bits (1677), Expect = 7.1e-183
Identity = 303/319 (94.98%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSRG 320
           RYRQISPFPRNRFDASSRG
Sbjct: 301 RYRQISPFPRNRFDASSRG 319

BLAST of CSPI04G17960 vs. NCBI nr
Match: XP_016899875.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucumis melo])

HSP 1 Score: 648.3 bits (1671), Expect = 3.5e-182
Identity = 302/318 (94.97%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLPEF 60
           MSLTPPQSTTNNRHTTTFQLYWCHQCHRT+TLASAH SEL CPRCFGQFIEE+QLTLPEF
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLPEF 60

Query: 61  NPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWR 120
           NP PE RLFEALSLMLNQPIRLFNNRTPNGNRHHPP HRFEEFDRRSFSDPEGDELPQWR
Sbjct: 61  NPLPEARLFEALSLMLNQPIRLFNNRTPNGNRHHPPLHRFEEFDRRSFSDPEGDELPQWR 120

Query: 121 RRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDE 180
           RRWRSRSLDERDNFG QPPNPN+SRTVIVFGPPDQLQPIQPI+PRR+SPRDYFTGPQLDE
Sbjct: 121 RRWRSRSLDERDNFGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFTGPQLDE 180

Query: 181 LIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEARELSCKH 240
           LIEELTQNDRPGPAPASEEAIERIP VKI AEHLKN+SHCPVCKEEFEVGGEARELSCKH
Sbjct: 181 LIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEARELSCKH 240

Query: 241 IYHSECIVPWLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCARWWSHLVPWPFRN 300
           IYHSECIVPWLRLHNSCPVCRQEMPS TPENEASSSSEEEGMGRRCARWWS LVPWPFRN
Sbjct: 241 IYHSECIVPWLRLHNSCPVCRQEMPSLTPENEASSSSEEEGMGRRCARWWSQLVPWPFRN 300

Query: 301 RYRQISPFPRNRFDASSR 319
           RYRQISPFPRNRFDASSR
Sbjct: 301 RYRQISPFPRNRFDASSR 318

BLAST of CSPI04G17960 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 6.7e-62
Identity = 143/332 (43.07%), Postives = 189/332 (56.93%), Query Frame = 0

Query: 1   MSLTPPQSTTN---NRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQL-- 60
           MSL+ P + T+   N    TF LYWC+ C R V +AS++PSE+ CPRC  QF+ E++   
Sbjct: 1   MSLSQPITRTDSAPNGAFRTFGLYWCYHCDRMVRIASSNPSEIACPRCLRQFVVEIETRQ 60

Query: 61  --------TLPEFNPSPEGRLFEALSLMLNQPIRLFNNRTPNGNRHHPPWHRFEEFDRRS 120
                     P F+ SPE RL EALSLM  +P  +       G     P+ R      R+
Sbjct: 61  RPRFTFNHATPPFDASPEARLLEALSLMF-EPATI-------GRFGADPFLRARS---RN 120

Query: 121 FSDPEGDELPQWRRRWRSRSLDERDNFGQQPPNPNRSRTVIVFGPPDQLQPIQPII---- 180
             +PE    PQ RRR    SLD  +N G   P     RT ++  P +   P+  II    
Sbjct: 121 ILEPESRPRPQHRRR---HSLDNVNNGGLPLPR----RTYVILRPNNPTSPLGNIIAPPN 180

Query: 181 ---PRRISPRDYFTG-PQLDELIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNE-S 240
              PR ++  DYFTG   L++LIE+LTQ+DRPGP PASE  I  +P+VKI  +HL N+ S
Sbjct: 181 QAPPRHVNSHDYFTGASSLEQLIEQLTQDDRPGPPPASEPTINSLPSVKITPQHLTNDMS 240

Query: 241 HCPVCKEEFEVGGEARELSCKHIYHSECIVPWLRLHNSCPVCRQEMP---SFTPENEASS 300
            C VC EEF VGG+A EL CKHIYH +CIVPWLRL+NSCP+CR+++P   +     E S+
Sbjct: 241 QCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPLVNTVAESRERSN 300

Query: 301 SSEEEGMGRRCARWWSHLVPWPFRNRYRQISP 308
              ++   RR  RW      WPFR RY+++SP
Sbjct: 301 PIRQDMPERRRPRWMQLGNIWPFRARYQRVSP 314

BLAST of CSPI04G17960 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-35
Identity = 108/315 (34.29%), Postives = 149/315 (47.30%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQL----TLPEFNPSPEGRLFEALSLML 80
           +WCH+C R V L +    +  C  C G F+EE+ +       +    P   L EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 81  NQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQ 140
                   +R    +       R       SFS                 +L     FG 
Sbjct: 68  -------RSRLAERSYDREISGRLGSAGSESFS-----------------NLAPLLIFGG 127

Query: 141 QPP-----NPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQ-NDR 200
           Q P       N S    V G    +      I R  +  DYF GP L+ELIE+L+     
Sbjct: 128 QAPFRLAGGDNSSVEAFVNGAAPGIG-----IARGTNAGDYFFGPGLEELIEQLSSGTHH 187

Query: 201 PGPAPASEEAIERIPTVKIEAEHLK-NESHCPVCKEEFEVGGEARELSCKHIYHSECIVP 260
            GP PA + +I+ +PT+KI  +HLK ++SHCPVCK+EFE+  EA+++ C HIYHS+CIVP
Sbjct: 188 RGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVP 247

Query: 261 WLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCAR---WWSHLVPWPFRNRYRQIS 320
           WL  HNSCPVCR+E+PS    +   SS      GR  +R    +S+L  WPFR+     S
Sbjct: 248 WLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNL--WPFRS---SSS 285

Query: 321 PFPRNRFDASSRGDA 322
              +NR D ++   A
Sbjct: 308 SSTQNRRDTNNTATA 285

BLAST of CSPI04G17960 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-35
Identity = 108/315 (34.29%), Postives = 149/315 (47.30%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQL----TLPEFNPSPEGRLFEALSLML 80
           +WCH+C R V L +    +  C  C G F+EE+ +       +    P   L EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 81  NQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQ 140
                   +R    +       R       SFS                 +L     FG 
Sbjct: 68  -------RSRLAERSYDREISGRLGSAGSESFS-----------------NLAPLLIFGG 127

Query: 141 QPP-----NPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQ-NDR 200
           Q P       N S    V G    +      I R  +  DYF GP L+ELIE+L+     
Sbjct: 128 QAPFRLAGGDNSSVEAFVNGAAPGIG-----IARGTNAGDYFFGPGLEELIEQLSSGTHH 187

Query: 201 PGPAPASEEAIERIPTVKIEAEHLK-NESHCPVCKEEFEVGGEARELSCKHIYHSECIVP 260
            GP PA + +I+ +PT+KI  +HLK ++SHCPVCK+EFE+  EA+++ C HIYHS+CIVP
Sbjct: 188 RGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVP 247

Query: 261 WLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCAR---WWSHLVPWPFRNRYRQIS 320
           WL  HNSCPVCR+E+PS    +   SS      GR  +R    +S+L  WPFR+     S
Sbjct: 248 WLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNL--WPFRS---SSS 285

Query: 321 PFPRNRFDASSRGDA 322
              +NR D ++   A
Sbjct: 308 SSTQNRRDTNNTATA 285

BLAST of CSPI04G17960 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.8e-35
Identity = 108/315 (34.29%), Postives = 149/315 (47.30%), Query Frame = 0

Query: 21  YWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQL----TLPEFNPSPEGRLFEALSLML 80
           +WCH+C R V L +    +  C  C G F+EE+ +       +    P   L EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 81  NQPIRLFNNRTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERDNFGQ 140
                   +R    +       R       SFS                 +L     FG 
Sbjct: 68  -------RSRLAERSYDREISGRLGSAGSESFS-----------------NLAPLLIFGG 127

Query: 141 QPP-----NPNRSRTVIVFGPPDQLQPIQPIIPRRISPRDYFTGPQLDELIEELTQ-NDR 200
           Q P       N S    V G    +      I R  +  DYF GP L+ELIE+L+     
Sbjct: 128 QAPFRLAGGDNSSVEAFVNGAAPGIG-----IARGTNAGDYFFGPGLEELIEQLSSGTHH 187

Query: 201 PGPAPASEEAIERIPTVKIEAEHLK-NESHCPVCKEEFEVGGEARELSCKHIYHSECIVP 260
            GP PA + +I+ +PT+KI  +HLK ++SHCPVCK+EFE+  EA+++ C HIYHS+CIVP
Sbjct: 188 RGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVP 247

Query: 261 WLRLHNSCPVCRQEMPSFTPENEASSSSEEEGMGRRCAR---WWSHLVPWPFRNRYRQIS 320
           WL  HNSCPVCR+E+PS    +   SS      GR  +R    +S+L  WPFR+     S
Sbjct: 248 WLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGRENSRRRNIFSNL--WPFRS---SSS 285

Query: 321 PFPRNRFDASSRGDA 322
              +NR D ++   A
Sbjct: 308 SSTQNRRDTNNTATA 285

BLAST of CSPI04G17960 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-32
Identity = 97/302 (32.12%), Postives = 151/302 (50.00%), Query Frame = 0

Query: 19  QLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLP----EFNPSPEGRLFEALSL 78
           ++++C+QC++TVT++ +  ++  CP C   F+EE +   P     FNP+     F     
Sbjct: 19  KMFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADP 78

Query: 79  MLNQPIRLFNN--RTPNGNRHHPPWHRFEEFDRRSFSDPEGDELPQWRRRWRSRSLDERD 138
                  +F +    P+G             D  S   P     PQ R   ++   D  D
Sbjct: 79  FSTLLPLIFGSSAAAPSG------------MDFMSLFGPSMQ--PQARSTQQNPQSDAFD 138

Query: 139 NFGQQPPNPNRSRTVIVFGPPDQL----QPIQPIIPRRISPRDYFTGPQLDELIEELTQN 198
            F       N  +T+   G   +      P  P      +  DYF GP L++LI++L +N
Sbjct: 139 PFTFL---QNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEN 198

Query: 199 D--RPGPAPASEEAIERIPTVKIEAEHLKNE-SHCPVCKEEFEVGGEARELSCKHIYHSE 258
           D  R G  PAS+ AI+ +PTVK+  + LK+E + C VC +EFE G + +++ CKH++H +
Sbjct: 199 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 258

Query: 259 CIVPWLRLHNSCPVCRQEMPSFTPENE-ASSSSEEEGMGRRCARW-----WSHLVPWPFR 302
           C++PWL LHNSCPVCR E+P+  P+ E  S  S+  G G+          +S  +PWPFR
Sbjct: 259 CLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQLPWPFR 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AK42.6e-3434.29E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
P0CH306.3e-3333.55E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN71.6e-3132.12E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
O221973.5e-3135.95Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T53.1e-2430.69E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L0L21.2e-19199.37RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE... [more]
A0A5A7TUM62.4e-18495.02RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B9M63.5e-18394.98RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 ... [more]
A0A1S4DVY71.7e-18294.97RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 ... [more]
A0A6J1IKH71.3e-14277.50RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 P... [more]
Match NameE-valueIdentityDescription
XP_004142693.24.9e-19299.37E3 ubiquitin-protein ligase RZF1 [Cucumis sativus][more]
KGN54579.12.4e-19199.37hypothetical protein Csa_012041 [Cucumis sativus][more]
KAA0045221.14.9e-18495.02E3 ubiquitin-protein ligase RING1 isoform X2 [Cucumis melo var. makuwa] >TYK1924... [more]
XP_008444136.17.1e-18394.98PREDICTED: E3 ubiquitin-protein ligase RING1 isoform X2 [Cucumis melo][more]
XP_016899875.13.5e-18294.97PREDICTED: E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G60360.16.7e-6243.07RING/U-box superfamily protein [more]
AT3G56580.21.8e-3534.29RING/U-box superfamily protein [more]
AT3G56580.11.8e-3534.29RING/U-box superfamily protein [more]
AT3G56580.31.8e-3534.29RING/U-box superfamily protein [more]
AT3G19950.11.1e-3232.12RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 220..260
e-value: 1.3E-6
score: 37.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 219..261
e-value: 4.8E-11
score: 42.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 220..261
score: 12.135206
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 183..267
e-value: 2.2E-22
score: 80.5
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 19..52
e-value: 1.2E-12
score: 47.9
NoneNo IPR availablePANTHERPTHR15710:SF29SUBFAMILY NOT NAMEDcoord: 14..276
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 14..276
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 220..261
e-value: 1.30979E-21
score: 84.2916
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 200..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17960.2CSPI04G17960.2mRNA
CSPI04G17960.1CSPI04G17960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity