CSPI04G17590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G17590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description1-aminocyclopropane-1-carboxylate oxidase
LocationChr4: 15064363 .. 15066390 (-)
RNA-Seq ExpressionCSPI04G17590
SyntenyCSPI04G17590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATAAACTACTACTCTCTCAATCAAATTTCATCCCTTCCAAAAGAAAAAAAAACCTGTGTTTTCAGCTTTCTAATGGCAATTCCAGTCATTGATTTCTCTAAGCTTAATGGGCAAGAAAGGGCCAAGACATTGGCTCAGATAGCCAATGGCTGCGAAGAATGGGGATTCTTTCAGGTACTTTAACTATTAGTCTTTTGTCTGCAAGTTTCAGGTTGATGCCAAGGGCATGTTTTTTCACGTATTCAAAATCAATTCAAAACATATTTTTAATTATTCAAAGTTGATTTTTCAGGACATTTCAATTGTGCTTAAAAATATAAAATATAACTTTAAATTAATTTTTAACGGTTGATATGTTTCAAAGTGATTTTATCATGTTCAAAGTATGTCCCAAACTATAGTACCTGTAGTGTAACGGCTGGTGTTCTAGTGTTTAAAGTGCCACTTGGTAGCTATATGGTTATTGGTTTTTGAAAATCGTGCCTCATTTCTCACTTCTTCTTACTTCTAGTTTTCATAGTTCACTTGAATTCTAAATCAATTTCTAAAAACAAAAATAAGTATATGAAGACTACTTTTTTTTTTTTTTGTTCTTTTTTCTTATTTTGAAACTTTAACTGACTTTAAAAACACTTTAAAAACACTTCTAACACATAGACAACAAAACAAAGAAATTAAAAGATGAAAGTGGTATTTTATAAGCTTAATTTTCAAATATTAAAAACCAACAGTTACCAAACGGGACATAAATATTTGGATTATGGTTTGATTAAAAGGAATATATGTTATTTGAATACGTCAGCTGGTGAACCATGGGATTCCTGAAGAGCTCCTAGAGAGGGTGAAGAAGGTTTCTTCAGAGTGCTACAAGGTGGAAAGAGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTTGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATGACAACGAATGGCCCTCCAACCTTCCTGGATTTAAGTAAGCATTCAACTTTCATGAAAATTCAGTGAAAATAGGGCATTGAAATCAGATTCTTCTTAAAAACAGGAAATAATATATGTATATGAATCCAATTATGGCAGGGAAACCATGAGAGAGTACAGATCGGAGCTGAGAAAACTAGCTGAAAAGGTGATGGCAGTAATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTTGGAACCAAAATCAGCCATTACCCACCATGTCCACACCCAGAGCTAGTGAATGGCCTCCGTGCCCACACCGATGCCGGCGGTGTCATCTTGCTCTTCCAAGACGATCAGGTGGGAGGCCTTCAAATCTTGAAAGATGGGCAGTGGATTGATGTGCAGCCATTTCCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGGAGATACAAGAGTGTTTGGCATCGGGTTTTAGCCTCACCGAATGGGAACAGGAGATCAATTGCTTCATTTTACAATCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAAGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTATATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGAACCTAGGTTTCAAGCTGTCAGGGCAATGTGAGGAGCATAAATCAAAAGGAGTTGGTGTTAAATTTCACTCTCCCGATGGCCTTTCATTCAAGGGTTTTCATATAGTAGTGACAAGATTTGAATAATGCTACTTCTTTCCCCTCCCCCCCACAAAAAGAAAGAACAAAAAAAGGAACCACTTTCCCTTCAGGGTTTGTGTGTGTTTGCTTTAGTGAATTTGATGTTGTTTCTCCATTAATTGTTGTATGTTATGGAATGTGTTCGTAAGACTAATAATATTCAGTATTGGGTATTGTTATTCAACATTGTATTTTGGACTTGCA

mRNA sequence

ACATAAACTACTACTCTCTCAATCAAATTTCATCCCTTCCAAAAGAAAAAAAAACCTGTGTTTTCAGCTTTCTAATGGCAATTCCAGTCATTGATTTCTCTAAGCTTAATGGGCAAGAAAGGGCCAAGACATTGGCTCAGATAGCCAATGGCTGCGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCTGAAGAGCTCCTAGAGAGGGTGAAGAAGGTTTCTTCAGAGTGCTACAAGGTGGAAAGAGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTTGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATGACAACGAATGGCCCTCCAACCTTCCTGGATTTAAGGAAACCATGAGAGAGTACAGATCGGAGCTGAGAAAACTAGCTGAAAAGGTGATGGCAGTAATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTTGGAACCAAAATCAGCCATTACCCACCATGTCCACACCCAGAGCTAGTGAATGGCCTCCGTGCCCACACCGATGCCGGCGGTGTCATCTTGCTCTTCCAAGACGATCAGGTGGGAGGCCTTCAAATCTTGAAAGATGGGCAGTGGATTGATGTGCAGCCATTTCCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGGAGATACAAGAGTGTTTGGCATCGGGTTTTAGCCTCACCGAATGGGAACAGGAGATCAATTGCTTCATTTTACAATCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAAGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTATATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGAACCTAGGTTTCAAGCTGTCAGGGCAATGTGAGGAGCATAAATCAAAAGGAGTTGGTGTTAAATTTCACTCTCCCGATGGCCTTTCATTCAAGGGTTTTCATATAGTAGTGACAAGATTTGAATAATGCTACTTCTTTCCCCTCCCCCCCACAAAAAGAAAGAACAAAAAAAGGAACCACTTTCCCTTCAGGGTTTGTGTGTGTTTGCTTTAGTGAATTTGATGTTGTTTCTCCATTAATTGTTGTATGTTATGGAATGTGTTCGTAAGACTAATAATATTCAGTATTGGGTATTGTTATTCAACATTGTATTTTGGACTTGCA

Coding sequence (CDS)

ATGGCAATTCCAGTCATTGATTTCTCTAAGCTTAATGGGCAAGAAAGGGCCAAGACATTGGCTCAGATAGCCAATGGCTGCGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCTGAAGAGCTCCTAGAGAGGGTGAAGAAGGTTTCTTCAGAGTGCTACAAGGTGGAAAGAGAAGAAAGTTTCAAGAGTTCAAAACCTGTGAAGCTCCTGAATGATTTGCTTGAGAACAAGAGTGGTGAGAAGCTGGAAAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGATGACAACGAATGGCCCTCCAACCTTCCTGGATTTAAGGAAACCATGAGAGAGTACAGATCGGAGCTGAGAAAACTAGCTGAAAAGGTGATGGCAGTAATGGATGAGAACTTAGGTTTACCTGAAGGATACATAAAGGCTGCCTTCAATGGTGGCGAAGGGCTGGAAAAGGCCTTCTTTGGAACCAAAATCAGCCATTACCCACCATGTCCACACCCAGAGCTAGTGAATGGCCTCCGTGCCCACACCGATGCCGGCGGTGTCATCTTGCTCTTCCAAGACGATCAGGTGGGAGGCCTTCAAATCTTGAAAGATGGGCAGTGGATTGATGTGCAGCCATTTCCCAACTCCATAGTCATCAACACAGGTGATCAGATTGAGGTCCTGAGCAATGGGAGATACAAGAGTGTTTGGCATCGGGTTTTAGCCTCACCGAATGGGAACAGGAGATCAATTGCTTCATTTTACAATCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAAGTGGAAGAAGGATATCCCAAGTTTGTGTTTGGAGACTATATGTCAGTTTATGCTGAGCAGAAGTTCCTCCCAAAGGAACCTAGGTTTCAAGCTGTCAGGGCAATGTGA

Protein sequence

MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVEREESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKEPRFQAVRAM*
Homology
BLAST of CSPI04G17590 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.5e-135
Identity = 233/313 (74.44%), Postives = 266/313 (84.98%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ER KTL++IA  CEEWGFFQLVNHGIP ELL +VKK+SS+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDDNEWPSNLPGFKETMREYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H+ NEWPSN+   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELRKLAEKVMAVMDENLGLPEGYIKAAFNGG--EGLEKAFFGTKISHYPPCPHPELVNGL 180
           E+RKLA K+M VMDENLGLP+GYIK AFN G  +G E AFFGTK+SHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDD+  GLQ+LKDG+WIDVQP PN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E+ YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQAVRAM 310
           +PKEPRF AV+++
Sbjct: 301 MPKEPRFLAVKSL 307

BLAST of CSPI04G17590 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 2.2e-131
Identity = 229/311 (73.63%), Postives = 268/311 (86.17%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKL+G+ERA+T+A+IANGCEEWGFFQLVNHGIP ELLERVKKVSSECYK+ R
Sbjct: 1   MAIPVIDFSKLDGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKL-R 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EE F+ SKPV+LL+ L++   G++L+N+DWEDVF+L DDNEWPSN P F+ETM+EYR E+
Sbjct: 61  EERFEGSKPVQLLDTLVKEGDGQRLDNVDWEDVFVLQDDNEWPSNPPDFEETMKEYREEI 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           RKLAEK+M VMDENLG  +G IK AF+ G+G    FFGTK+SHYPPCP  +LV GLRAHT
Sbjct: 121 RKLAEKMMEVMDENLGFEKGCIKKAFS-GDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQ+LKDG+WIDVQP  ++IVINTGDQIEVLSNGRYKS WHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSNGRYKSAWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEG--YPKFVFGDYMSVYAEQKFLP 300
           + +GNRRSIASFYNPS++ATIAPAA     A +E      YPKF+FGDYM VYA+QK+ P
Sbjct: 241 TSHGNRRSIASFYNPSLKATIAPAA---GAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 300

Query: 301 KEPRFQAVRAM 310
           KEPRF+AVRA+
Sbjct: 301 KEPRFEAVRAI 306

BLAST of CSPI04G17590 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.8e-91
Identity = 164/313 (52.40%), Postives = 220/313 (70.29%), Query Frame = 0

Query: 2   AIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVERE 61
           A PVID  KLNG+ERA T+ +I + CE WGFF+LVNHGI  EL++ V++++ E Y    E
Sbjct: 3   AFPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DDNEWPSNLPGFKETMREY 121
           + FK     K L  +       ++ +LDWE  F L      + +E P      ++ M+E+
Sbjct: 63  QRFKEMVATKGLEAV-----QSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEF 122

Query: 122 RSELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGL 181
             +L KLAE+++ ++ EN+GL +GY+K AF G +G     FGTK+S+YPPCP PEL+ GL
Sbjct: 123 AEKLEKLAEQLLDLLCENVGLEKGYLKKAFYGSKG---PTFGTKVSNYPPCPRPELIKGL 182

Query: 182 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQD++V GLQ+LKDG+WIDV P  +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMH 242

Query: 242 RVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 301
           RV+A P+GNR SIASFYNP  +A + PA  LVDK   + ++ YPKFVF DYM +YA  KF
Sbjct: 243 RVIAQPDGNRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKF 302

Query: 302 LPKEPRFQAVRAM 310
             KEPRF+A++AM
Sbjct: 303 QAKEPRFEAMKAM 307

BLAST of CSPI04G17590 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.0e-88
Identity = 160/311 (51.45%), Postives = 216/311 (69.45%), Query Frame = 0

Query: 4   PVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVEREES 63
           P+I   K+NG ERA T+  I + CE WGFF+LVNHGIP E+++ V+K++   YK   E+ 
Sbjct: 5   PIISLDKVNGVERAATMEMIKDACENWGFFELVNHGIPREVMDTVEKMTKGHYKKCMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-----HDDNEWPSNLPGFKETMREYRS 123
           FK     K L  +       ++ ++DWE  F L      + +E P     ++E MR++  
Sbjct: 65  FKELVASKALEGV-----QAEVTDMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAK 124

Query: 124 ELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRA 183
            L KLAE+++ ++ ENLGL +GY+K AF G +G     FGTK+S+YPPCP P+L+ GLRA
Sbjct: 125 RLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           HTDAGG+ILLFQDD+V GLQ+LKDGQWIDV P  +SIV+N GDQ+EV++NG+YKSV HRV
Sbjct: 185 HTDAGGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVMHRV 244

Query: 244 LASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLP 303
           +A  +G R S+ASFYNP  +A I PA  LV+K  +E ++ YPKFVF DYM +YA  KF  
Sbjct: 245 IAQKDGARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKLYAGLKFQA 304

Query: 304 KEPRFQAVRAM 310
           KEPRF+A++AM
Sbjct: 305 KEPRFEAMKAM 307

BLAST of CSPI04G17590 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 3.9e-88
Identity = 159/310 (51.29%), Postives = 220/310 (70.97%), Query Frame = 0

Query: 4   PVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVEREES 63
           PV+D S +NG+ERA TL +I + CE WGFF+LVNHG+  ELL+ V+K++ + YK   E+ 
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DDNEWPSNLPGFKETMREYRS 123
           FK     K L+D+       ++ +LDWE  F L      + +E P     +++TM+E+  
Sbjct: 65  FKEMVAAKGLDDV-----QSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAV 124

Query: 124 ELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRA 183
           EL KLAEK++ ++ ENLGL +GY+K  F G +G     FGTK+S+YPPCP P+L+ GLRA
Sbjct: 125 ELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           H+DAGG+ILLFQDD+V GLQ+LKDG+W+DV P  +SIVIN GDQIEV++NG+YKSV HRV
Sbjct: 185 HSDAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRV 244

Query: 244 LASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLP 303
           +A  +G R SIASFYNP  ++ I+PA  +++K  ++    YPKFVF DYM +Y+  KF  
Sbjct: 245 IAQSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPT-YPKFVFDDYMKLYSGLKFQA 304

Query: 304 KEPRFQAVRA 309
           KEPRF+A++A
Sbjct: 305 KEPRFEAMKA 305

BLAST of CSPI04G17590 vs. ExPASy TrEMBL
Match: A0A0A0L1U8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361270 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 6.5e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. ExPASy TrEMBL
Match: A0A5A7UFF3 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00040 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 3.9e-176
Identity = 304/309 (98.38%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTKISHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. ExPASy TrEMBL
Match: A0A1S3B9W8 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487610 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 3.9e-176
Identity = 304/309 (98.38%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTKISHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. ExPASy TrEMBL
Match: A0A6J1FFA0 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443415 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-170
Identity = 290/309 (93.85%), Postives = 304/309 (98.38%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKL+G+ERAKTL QIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP  NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRA+
Sbjct: 301 PRFQAVRAV 309

BLAST of CSPI04G17590 vs. ExPASy TrEMBL
Match: A0A6J1BWX3 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Momordica charantia OX=3673 GN=LOC111006422 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-170
Identity = 290/309 (93.85%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIP+IDFSKLNG+ERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLA+KVMAVMDENLGLPEGYI AAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQSVRAM 309

BLAST of CSPI04G17590 vs. NCBI nr
Match: XP_004142637.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucumis sativus] >KAE8637421.1 hypothetical protein CSA_021607 [Cucumis sativus] >KGN54542.1 hypothetical protein Csa_012615 [Cucumis sativus])

HSP 1 Score: 636.3 bits (1640), Expect = 1.3e-178
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. NCBI nr
Match: XP_008444197.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA0052209.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa] >TYK01776.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 8.1e-176
Identity = 304/309 (98.38%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFN GEGLEKAFFGTKISHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. NCBI nr
Match: XP_038898758.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Benincasa hispida])

HSP 1 Score: 618.2 bits (1593), Expect = 3.8e-173
Identity = 297/309 (96.12%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ER KTLAQIANGCEEWGFFQLVNHGIPEELLERVKK+SSE YKVER
Sbjct: 1   MAIPVIDFSKLNGEERPKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKISSEYYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETMREYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMREYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDD+VGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDEVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+GYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEKGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQAVRAM
Sbjct: 301 PRFQAVRAM 309

BLAST of CSPI04G17590 vs. NCBI nr
Match: XP_022134061.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Momordica charantia])

HSP 1 Score: 607.8 bits (1566), Expect = 5.1e-170
Identity = 290/309 (93.85%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIP+IDFSKLNG+ERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLA+KVMAVMDENLGLPEGYI AAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQAVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQSVRAM 309

BLAST of CSPI04G17590 vs. NCBI nr
Match: KAG7024737.1 (1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 607.8 bits (1566), Expect = 5.1e-170
Identity = 290/309 (93.85%), Postives = 304/309 (98.38%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKL+G+ERAKTL QIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 31  MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 90

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+NEWPSN+PGFKETM EYRSEL
Sbjct: 91  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 150

Query: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180
           +KLAEKVMA+MDENLGLPEGYIKAAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 151 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 210

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP  NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 211 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 270

Query: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300
           +PNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 271 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 330

Query: 301 PRFQAVRAM 310
           PRFQAVRA+
Sbjct: 331 PRFQAVRAV 339

BLAST of CSPI04G17590 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 483.0 bits (1242), Expect = 1.8e-136
Identity = 233/313 (74.44%), Postives = 266/313 (84.98%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           MAIPVIDFSKLNG+ER KTL++IA  CEEWGFFQLVNHGIP ELL +VKK+SS+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDDNEWPSNLPGFKETMREYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H+ NEWPSN+   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELRKLAEKVMAVMDENLGLPEGYIKAAFNGG--EGLEKAFFGTKISHYPPCPHPELVNGL 180
           E+RKLA K+M VMDENLGLP+GYIK AFN G  +G E AFFGTK+SHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDD+  GLQ+LKDG+WIDVQP PN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E+ YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQAVRAM 310
           +PKEPRF AV+++
Sbjct: 301 MPKEPRFLAVKSL 307

BLAST of CSPI04G17590 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 159/318 (50.00%), Postives = 217/318 (68.24%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           M  PV+D SKLNG+ER +T+A I   CE WGFF++VNHG+P +L+++++K++ + YK  +
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EESFKSSKPVKLLNDLLENKSGEKL----ENLDWEDVFLLHDDNEWPSNLPGFKETMREY 120
           E+ F         ND+L++K  + L    E++DWE  F +    +  SNL    +   EY
Sbjct: 65  EQKF---------NDMLKSKGLDNLETEVEDVDWESTFYVRHLPQ--SNLNDISDVSDEY 124

Query: 121 RS-------ELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPH 180
           R+        L  LAE ++ ++ ENLGL +GY+K  F+G +G     FGTK+S+YPPCP 
Sbjct: 125 RTAMKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKG---PTFGTKVSNYPPCPK 184

Query: 181 PELVNGLRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNG 240
           PE++ GLRAHTDAGG+ILLFQDD+V GLQ+LKDG WIDV P  +SIVIN GDQ+EV++NG
Sbjct: 185 PEMIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNG 244

Query: 241 RYKSVWHRVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMS 300
           +YKSV HRV+    GNR S+ASFYNP  +A I+PA  LV+K ++     YP FVF DYM 
Sbjct: 245 KYKSVLHRVVTQQEGNRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMK 303

Query: 301 VYAEQKFLPKEPRFQAVR 308
           +YA  KF PKEPRF A++
Sbjct: 305 LYAGVKFQPKEPRFAAMK 303

BLAST of CSPI04G17590 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.7e-86
Identity = 161/311 (51.77%), Postives = 212/311 (68.17%), Query Frame = 0

Query: 4   PVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVEREES 63
           PVID SKLNG+ER +T+A I + C+ WGFF+LVNHG+P +L++ +++++ E YK   E+ 
Sbjct: 8   PVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQK 67

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYR------ 123
           FK     K L D LE     ++E++DWE  F LH   +  SNL    +   EYR      
Sbjct: 68  FKEMLRSKGL-DTLET----EVEDVDWESTFYLHHLPQ--SNLYDIPDMSNEYRLAMKDF 127

Query: 124 -SELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGL 183
              L  LAE+++ ++ ENLGL +GY+K  F+G  G     F TK+S+YPPCP PE++ GL
Sbjct: 128 GKRLEILAEELLDLLCENLGLEKGYLKKVFHGTTG---PTFATKLSNYPPCPKPEMIKGL 187

Query: 184 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWH 243
           RAHTDAGG+ILLFQDD+V GLQ+LKDG W+DV P  +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 188 RAHTDAGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMH 247

Query: 244 RVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKF 303
           RV+    GNR SIASFYNP  +A I+PA  LVDK     +  YP FVF DYM +YA  KF
Sbjct: 248 RVMTQKEGNRMSIASFYNPGSDAEISPATSLVDK-----DSKYPSFVFDDYMKLYAGLKF 303

Query: 304 LPKEPRFQAVR 308
             KEPRF+A++
Sbjct: 308 QAKEPRFEAMK 303

BLAST of CSPI04G17590 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 314.7 bits (805), Expect = 8.3e-86
Identity = 155/315 (49.21%), Postives = 223/315 (70.79%), Query Frame = 0

Query: 2   AIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVERE 61
           + P+I+  KLNG+ERA T+ +I + CE WGFF+ VNHGI  ELL++V+K++ E YK   E
Sbjct: 3   SFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-----HDDNEWPSNLPGFKETMREY 121
           E FK S   + L+ L       ++ ++DWE  F L      + ++ P     ++  M+++
Sbjct: 63  ERFKESIKNRGLDSL-----RSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDF 122

Query: 122 RSELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGL 181
             ++ KL+E+++ ++ ENLGL +GY+K  F G    ++  FGTK+S+YPPCP+P+LV GL
Sbjct: 123 AGKIEKLSEELLDLLCENLGLEKGYLKKVFYGS---KRPTFGTKVSNYPPCPNPDLVKGL 182

Query: 182 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQDD+V GLQ+LKDG+W+DV P  +SIV+N GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEH 242

Query: 242 RVLASPNG-NRRSIASFYNPSMEATIAPAAQLVDK-ANQEVEEGYPKFVFGDYMSVYAEQ 301
           RVL+  +G  R SIASFYNP  ++ I PA +L+ K A +E +E YP+FVF DYM +Y+  
Sbjct: 243 RVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAV 302

Query: 302 KFLPKEPRFQAVRAM 310
           KF  KEPRF+A++AM
Sbjct: 303 KFQAKEPRFEAMKAM 309

BLAST of CSPI04G17590 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 268.9 bits (686), Expect = 5.2e-72
Identity = 142/316 (44.94%), Postives = 203/316 (64.24%), Query Frame = 0

Query: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60
           M IPVIDF++L+G++R+KT++ + + C++WGFF + NHGI +EL+E+VKK+ +  Y+   
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-HDDNEWPSNLPGFKE----TMRE 120
           +E F  S+ VK L++        K  + DWE  F + H        +P   E    TM E
Sbjct: 69  KEKFYQSEMVKALSE-------GKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDE 128

Query: 121 YRSELRKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNG 180
           Y  +L K AE++  +M ENLGL +  I  AF+G +G     FGTK++ YP CP PEL+ G
Sbjct: 129 YVCQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKG---PAFGTKVAKYPECPRPELMRG 188

Query: 181 LRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPFP-NSIVINTGDQIEVLSNGRYKSV 240
           LR HTDAGG+ILL QDDQV GL+  KDG+W+ + P   N+I +NTGDQ+E+LSNGRYKSV
Sbjct: 189 LREHTDAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSV 248

Query: 241 WHRVLASPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPK-FVFGDYMSVYAE 300
            HRV+   +G+R SIA+FYNP+ +A I+PA +L+          YP  + F DY+ +Y+ 
Sbjct: 249 VHRVMTVKHGSRLSIATFYNPAGDAIISPAPKLL----------YPSGYRFQDYLKLYST 304

Query: 301 QKFLPKEPRFQAVRAM 310
            KF  K PR + ++ M
Sbjct: 309 TKFGDKGPRLETMKKM 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPW42.5e-13574.441-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q9FR992.2e-13173.631-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=... [more]
P312373.8e-9152.401-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ... [more]
Q085063.0e-8851.451-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 P... [more]
Q009853.9e-8851.291-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1U86.5e-179100.00Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A5A7UFF33.9e-17698.381-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B9W83.9e-17698.381-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487... [more]
A0A6J1FFA02.5e-17093.851-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschat... [more]
A0A6J1BWX32.5e-17093.851-aminocyclopropane-1-carboxylate oxidase 5 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004142637.11.3e-178100.001-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-... [more]
XP_008444197.18.1e-17698.38PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA005220... [more]
XP_038898758.13.8e-17396.121-aminocyclopropane-1-carboxylate oxidase 5 [Benincasa hispida][more]
XP_022134061.15.1e-17093.851-aminocyclopropane-1-carboxylate oxidase 5 [Momordica charantia][more]
KAG7024737.15.1e-17093.851-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT1G77330.11.8e-13674.442-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G62380.11.2e-8750.00ACC oxidase 2 [more]
AT1G12010.11.7e-8651.772-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G05010.18.3e-8649.21ethylene-forming enzyme [more]
AT2G19590.15.2e-7244.94ACC oxidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 113..133
NoneNo IPR availablePANTHERPTHR47991:SF1201-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 5-LIKEcoord: 2..307
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 2..307
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 1..302
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 163..255
e-value: 5.0E-26
score: 91.1
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 3..100
e-value: 9.8E-19
score: 68.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..309
e-value: 2.2E-96
score: 324.9
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 151..255
score: 13.209667

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G17590.1CSPI04G17590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901576 organic substance biosynthetic process
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen