CSPI04G16850 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G16850
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase Praja-2
Location	Chr4: 14216848 .. 14220953 (+)
RNA-Seq Expression	CSPI04G16850
Synteny	CSPI04G16850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTGTGTAATCATCAGATCCATAAATCCATTTTTTTCTCCATACAAACAAACCAATTAGATGAAACCATCAACACCCACACACACACACAATTGAATCATTCAATCCCACTCTTCAAGTTTTTCGTTCAATCTGCCTTCTTTTGAACGAGAATGTTGGTGGGCCATTCTCAAATATCTCGCTCAAGCGCGCTTTGATTAGGGAACTTCTCGTCTTTCATCCTCCAATGGCGGCCTATCCTCCTACAATACAAACCACTTTCCACGAAGAACTCCATTGACCTTCTCCGCTTCACAATCCTACTCTTTTTCGTTTACCCTCTTCATTTCTCGTCATCTTACTACCCATTTTCACCCACCCACCCACCCACCGTAGATGTTTCTCAACAGCGTTCTTCAATTATTTGTTTCACTTTTCCTTTTTTCCCCTTAATCGCTTGGCCCTTTTCGTTTATTTGCTTGTTTTCTTCGTTTTACTCAAGTTTGCGGTTCGTTTTGTTCGATTCGATGAGATTCAGCCGGATGTGGGAGTTTTGAAGAACTGGAAGCAAACTGACTGGTAATGATCGCAACCCATCTTCGTGTTCTCTGATATCTTTGATGCATTTTTTTTGTTTTCCACAATTGTTTCTAGTGCCGTTTTTTTATGTTTCTTGGGTCAATCATTTGGTTTTGATTTGGGATTTCTGGTCTCTGACTTGCTTTTGATACTCCGGGGACTACGATTGTAGTGAGAATCATCGTATTAAGTGGTTGCATCGTCTTCTCCTTGAAGAAACCATTAGTTTACATTTGCCTTTTTTTGTTTACTAATTGATCTTCTGTAACTTCTCTTGAACTCTTGAAATCCATGTTAGCAACTGCTGAACACTGGTTATTGAGATTGTTGTTGGAGGGTTTTAGTATCAAAGTTGGGGATGGAACTTGTGGCTGACATTGGTTATTTATTAACTCTCATCATGGTGTGAGAAAATTCTTCCCACAATTTATAGTGTCTGGGGTGTTTGGGACGTTAAGTTGGGTTATGAAGTCCGGAGATAATATGTTGGAGTTAGGAAATTTGTATTTGGAACGTAGATTTGTAAAGATGGAGTTTCTCAATAAATAAATATGCAAAAGAGAGTAAAGGAAAAGATGGATTTCCTTGATAATGTGCAAAATTGAACACTCGTAAAATTGGTGGAGTTGAGTTACTTAGAAGTTTTCACAGCACAAGTATTGATCAAATCTTTTCATCGCTGGGAAGACAACAGAGGGATATTGGATTTATATCATGCTTTTGATGAAAGGACAGTTCAATTTAGTTTGAGATATGAATATGCTTTAGTTTTAAGCAAAGGGTGAAGTGAGGTTAGGAATGAAGGGGTAAAGGTGCTATCTAGTGCTGCTTATCTAATGTTATCTCTGGACTCTGTTGAATATTTTTTCATCACGGGGTTGTAGAGAGTGGGGAGTAGATGTCAGTCTACTTGAGGAAACAGCGACGAGGAGTGACTTGTGACGGGTTTCTTACTGAAGTAATAGATAAATGAAACTGACCAAATGAAAATGATAATATGGGACAACATCGTCAAGAACTGAGTACATATCCACAAGTGCAGAAAACTGTGACAAGGCAGCTTGTGGTTTCTTTGTAACAAATTTATGGTATAATGTTGAACCCGTGTGGTCAGTCCCTCGAGAGGTTTGATTGTCTCTTGTTTAAAAGGGCTATATAACTTGCATTTTGTGTTTCATTGTTGACTGATAACCTTACTTGAACAAGATCTGATCTATATATTGTATAATTGCGTCCTTGAGTTTTGAGGCGACAAGTATTCCATCTTCTACTTACTTCAACTTAGTTAAATCTGATTTGGTCTCCTATACTTTTAAACTTCATGTTGATTTTCGCTGTTAATTATTTGTGCTTTCTTGTTTCATTTCAGATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCAATGTTCATGTCATCATGAACAACAACCAAAGTGTCGATGAACATAGTCCAATACAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTTGTACCTGACAGTGATGCCCCTAGTGAACCTGAACCCATTGATATATGTGGCGATTGTAAATTTTTATTACTTGAAGATATTGAAACCCCTGTACGGGATTCTTATCACCGGATGACGCCTAGAGGAAGAAGAGCTATGCGTAGTAGTTCAGAGTCTCCTGAAAATCTTTTTTCACAAGAGTTCACCCATATGATTAACATGGTGAGACAAAGGCAATCCAGCATCTCCAGCCATGGGGATCGATATGCAGACGGTTCGAATGCAATGGATTTCATGCAGCACTCAAGTTCCCATACAACCCCTAATCAGTCTAGAAGATGGCGACAAGTATACTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGAGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTATAGACATTCTTACGGTGATAGTGATATTGTTTCCAATAGAACTTATGGGGGTGAGTCGGATGCTTCCTTGGATGATGGACATAGTTTCTTGGATACAGACGTTTTTATTCTGCCAGGTGAGGGATCTGACACCGATACAGACATTGATCCCATGCGTGCTGGTGTTGGACATTGGGACTCAAGCGATGAGGAGGATGATCATGGTGAATTTACGGAAGCCGAGACAGAGCAAGAAGTAGGATCTTCTGAAGCTAGACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTTCTCGGAGAGGTAACTCGAGCAATTGGAATGAACAGCTTTCTTCACCGGAATATGAGGGTTCAACTCCTGGGCATATCAGGAGGAATAGGCGATTTTATTCTATCACCAACTATGCACAATCAGAGTTACTATCTTATGTTGGGGATTCTGGTGACTATCTTGACAGACAAGGGTTTGAAGAACTGCTTGAACAAATTGCTGAGACAACAACCAGCTCAAGACGTGGAGCACCTCCTGCAGCCGTTTCTTTTGTGAAAAACCTCCCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATTTCCTGTGCAATATGCAAAGACTTCCTACATTTAGGCGTTGAAGTGAATCAACTCCCTTGCCTTCATCTCTACCACCCTTCCTGCATCTTGCCATGGTTGAGTGCTCGGAATTCATGTCCTCTGTGTCGTTATGAACTTCCAACCGATGATCGAGATTATGAAGAGGTAAAGCAAAGTAGTATCAATGCTGCAGCGGTCCATGGTCTTCAACCTCGGGTAGTTGGACTCGAAAATCCCAGTGGCTTGAATGGAGTTGGAGCAAATCCTGAGTTTGAAACTAGCCAAGAAGAGGATCAGAGGAACATAGACTCAGCAGTTATCAGTAGTAACAATTCGGGTAGAGGAGCTAGACGACGTTGGCTATTCGTTGCTGCTCCAATAGTTGGTCTTGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGTTTCGGGAGGTCAATTAACAGATGGGAGGCAGCTCGATGCTCACAGTGCAGAATCGTCAAACCAAAGAGAGAACAGAAGCAGGAGATGGTGGCAAATCGTCTAGCAACCACAAGGTTTAGTACAGAAAAATTGATCTAACACGAGCTTTCTTGTTTTTGTTGATTTCCCAGGATTGAAATCTAAAGGAATTTAGGATGTAGTTCGGTATTATTTCAGTTTTCTCAATGTGTTGAAGTAGAAGAGTTGATTTAAACGCACAAAGCAGCAACCTGTGCCTCGCTTGTGAGCCCAATGGGGGCAGTAATAAATAGGCCAATTGGTTCTGTTGTGCATGTATATACTTCTTACAATTTTTGTAAATAGAATTATATATTTTAATAATAATAAAAAAAAAAAAAAAAGGATTTGTTTTATATGATCGCCATCATTTTTCATTTCAAGAATGTATTTTTTATGAAAATTATTTGAAAGAATAAAACTTGAATTTTAAGTATACAGAATTTATTACAATAAATTAAAAAGAGGCAATTGGGCAAGAAATGCATTCTCCATCTTCGTAAACGTAAAAAGAAAAATTCCCAACTTCCCTCC

mRNA sequence

TCTGTGTAATCATCAGATCCATAAATCCATTTTTTTCTCCATACAAACAAACCAATTAGATGAAACCATCAACACCCACACACACACACAATTGAATCATTCAATCCCACTCTTCAAGTTTTTCGTTCAATCTGCCTTCTTTTGAACGAGAATGTTGGTGGGCCATTCTCAAATATCTCGCTCAAGCGCGCTTTGATTAGGGAACTTCTCGTCTTTCATCCTCCAATGGCGGCCTATCCTCCTACAATACAAACCACTTTCCACGAAGAACTCCATTGACCTTCTCCGCTTCACAATCCTACTCTTTTTCGTTTACCCTCTTCATTTCTCGTCATCTTACTACCCATTTTCACCCACCCACCCACCCACCGTAGATGTTTCTCAACAGCGTTCTTCAATTATTTGTTTCACTTTTCCTTTTTTCCCCTTAATCGCTTGGCCCTTTTCGTTTATTTGCTTGTTTTCTTCGTTTTACTCAAGTTTGCGGTTCGTTTTGTTCGATTCGATGAGATTCAGCCGGATGTGGGAGTTTTGAAGAACTGGAAGCAAACTGACTGATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCAATGTTCATGTCATCATGAACAACAACCAAAGTGTCGATGAACATAGTCCAATACAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTTGTACCTGACAGTGATGCCCCTAGTGAACCTGAACCCATTGATATATGTGGCGATTGTAAATTTTTATTACTTGAAGATATTGAAACCCCTGTACGGGATTCTTATCACCGGATGACGCCTAGAGGAAGAAGAGCTATGCGTAGTAGTTCAGAGTCTCCTGAAAATCTTTTTTCACAAGAGTTCACCCATATGATTAACATGGTGAGACAAAGGCAATCCAGCATCTCCAGCCATGGGGATCGATATGCAGACGGTTCGAATGCAATGGATTTCATGCAGCACTCAAGTTCCCATACAACCCCTAATCAGTCTAGAAGATGGCGACAAGTATACTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGAGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTATAGACATTCTTACGGTGATAGTGATATTGTTTCCAATAGAACTTATGGGGGTGAGTCGGATGCTTCCTTGGATGATGGACATAGTTTCTTGGATACAGACGTTTTTATTCTGCCAGGTGAGGGATCTGACACCGATACAGACATTGATCCCATGCGTGCTGGTGTTGGACATTGGGACTCAAGCGATGAGGAGGATGATCATGGTGAATTTACGGAAGCCGAGACAGAGCAAGAAGTAGGATCTTCTGAAGCTAGACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTTCTCGGAGAGGTAACTCGAGCAATTGGAATGAACAGCTTTCTTCACCGGAATATGAGGGTTCAACTCCTGGGCATATCAGGAGGAATAGGCGATTTTATTCTATCACCAACTATGCACAATCAGAGTTACTATCTTATGTTGGGGATTCTGGTGACTATCTTGACAGACAAGGGTTTGAAGAACTGCTTGAACAAATTGCTGAGACAACAACCAGCTCAAGACGTGGAGCACCTCCTGCAGCCGTTTCTTTTGTGAAAAACCTCCCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATTTCCTGTGCAATATGCAAAGACTTCCTACATTTAGGCGTTGAAGTGAATCAACTCCCTTGCCTTCATCTCTACCACCCTTCCTGCATCTTGCCATGGTTGAGTGCTCGGAATTCATGTCCTCTGTGTCGTTATGAACTTCCAACCGATGATCGAGATTATGAAGAGGTAAAGCAAAGTAGTATCAATGCTGCAGCGGTCCATGGTCTTCAACCTCGGGTAGTTGGACTCGAAAATCCCAGTGGCTTGAATGGAGTTGGAGCAAATCCTGAGTTTGAAACTAGCCAAGAAGAGGATCAGAGGAACATAGACTCAGCAGTTATCAGTAGTAACAATTCGGGTAGAGGAGCTAGACGACGTTGGCTATTCGTTGCTGCTCCAATAGTTGGTCTTGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGTTTCGGGAGGTCAATTAACAGATGGGAGGCAGCTCGATGCTCACAGTGCAGAATCGTCAAACCAAAGAGAGAACAGAAGCAGGAGATGGTGGCAAATCGTCTAGCAACCACAAGGTTTAGTACAGAAAAATTGATCTAACACGAGCTTTCTTGTTTTTGTTGATTTCCCAGGATTGAAATCTAAAGGAATTTAGGATGTAGTTCGGTATTATTTCAGTTTTCTCAATGTGTTGAAGTAGAAGAGTTGATTTAAACGCACAAAGCAGCAACCTGTGCCTCGCTTGTGAGCCCAATGGGGGCAGTAATAAATAGGCCAATTGGTTCTGTTGTGCATGTATATACTTCTTACAATTTTTGTAAATAGAATTATATATTTTAATAATAATAAAAAAAAAAAAAAAAGGATTTGTTTTATATGATCGCCATCATTTTTCATTTCAAGAATGTATTTTTTATGAAAATTATTTGAAAGAATAAAACTTGAATTTTAAGTATACAGAATTTATTACAATAAATTAAAAAGAGGCAATTGGGCAAGAAATGCATTCTCCATCTTCGTAAACGTAAAAAGAAAAATTCCCAACTTCCCTCC

Coding sequence (CDS)

ATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCAATGTTCATGTCATCATGAACAACAACCAAAGTGTCGATGAACATAGTCCAATACAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTTGTACCTGACAGTGATGCCCCTAGTGAACCTGAACCCATTGATATATGTGGCGATTGTAAATTTTTATTACTTGAAGATATTGAAACCCCTGTACGGGATTCTTATCACCGGATGACGCCTAGAGGAAGAAGAGCTATGCGTAGTAGTTCAGAGTCTCCTGAAAATCTTTTTTCACAAGAGTTCACCCATATGATTAACATGGTGAGACAAAGGCAATCCAGCATCTCCAGCCATGGGGATCGATATGCAGACGGTTCGAATGCAATGGATTTCATGCAGCACTCAAGTTCCCATACAACCCCTAATCAGTCTAGAAGATGGCGACAAGTATACTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGAGGAGAGTGAATCAAATTTCAGTTTTGGGCCTTATAGACATTCTTACGGTGATAGTGATATTGTTTCCAATAGAACTTATGGGGGTGAGTCGGATGCTTCCTTGGATGATGGACATAGTTTCTTGGATACAGACGTTTTTATTCTGCCAGGTGAGGGATCTGACACCGATACAGACATTGATCCCATGCGTGCTGGTGTTGGACATTGGGACTCAAGCGATGAGGAGGATGATCATGGTGAATTTACGGAAGCCGAGACAGAGCAAGAAGTAGGATCTTCTGAAGCTAGACCTCAACTTCAAAATTTTCATGTTTCTAGTGCTTCTCGGAGAGGTAACTCGAGCAATTGGAATGAACAGCTTTCTTCACCGGAATATGAGGGTTCAACTCCTGGGCATATCAGGAGGAATAGGCGATTTTATTCTATCACCAACTATGCACAATCAGAGTTACTATCTTATGTTGGGGATTCTGGTGACTATCTTGACAGACAAGGGTTTGAAGAACTGCTTGAACAAATTGCTGAGACAACAACCAGCTCAAGACGTGGAGCACCTCCTGCAGCCGTTTCTTTTGTGAAAAACCTCCCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATTTCCTGTGCAATATGCAAAGACTTCCTACATTTAGGCGTTGAAGTGAATCAACTCCCTTGCCTTCATCTCTACCACCCTTCCTGCATCTTGCCATGGTTGAGTGCTCGGAATTCATGTCCTCTGTGTCGTTATGAACTTCCAACCGATGATCGAGATTATGAAGAGGTAAAGCAAAGTAGTATCAATGCTGCAGCGGTCCATGGTCTTCAACCTCGGGTAGTTGGACTCGAAAATCCCAGTGGCTTGAATGGAGTTGGAGCAAATCCTGAGTTTGAAACTAGCCAAGAAGAGGATCAGAGGAACATAGACTCAGCAGTTATCAGTAGTAACAATTCGGGTAGAGGAGCTAGACGACGTTGGCTATTCGTTGCTGCTCCAATAGTTGGTCTTGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGTTTCGGGAGGTCAATTAACAGATGGGAGGCAGCTCGATGCTCACAGTGCAGAATCGTCAAACCAAAGAGAGAACAGAAGCAGGAGATGGTGGCAAATCGTCTAG

Protein sequence

MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDICGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSISSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSFGPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEEDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQLDAHSAESSNQRENRSRRWWQIV*

Homology

[Close]

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 9.1e-27
Identity = 54/116 (46.55%), Postives = 75/116 (64.66%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G+ GDY    G E+L++Q+AE    +R G PPA+ S +  LP + ++K+ LK +   CA+
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAE-NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAV 219

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSS 443
           C D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   Q S
Sbjct: 220 CMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGS 274

[Close]

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 7.7e-26
Identity = 53/107 (49.53%), Postives = 70/107 (65.42%), Query Frame = 0


Query: 330 GDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKD 389
           GDY    G E+L++Q+AE    +R G PPA+ S ++ LP + I+K +L  +   CA+C D
Sbjct: 171 GDYFIGPGLEQLIQQLAE-NDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMD 230

Query: 390 FLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYE 437
               G E  Q+PC HLYH  C+LPWL   NSCP+CR+ELPTDD DYE
Sbjct: 231 DFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYE 276

[Close]

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 8.5e-25
Identity = 58/140 (41.43%), Postives = 78/140 (55.71%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G++GDY    G EEL EQ++  TT  RRG PPA  S +  LP + I++ HL+    +C +
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT--RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPV 194

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELP------------TDDRD 446
           CKD   LG E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+
Sbjct: 195 CKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRN 254

Query: 447 YEEVKQSSINAAAVHGLQPR 455
           Y     SS + +  +G + R
Sbjct: 255 YRSSSSSSSSNSRENGNERR 272

[Close]

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.4e-22
Identity = 59/155 (38.06%), Postives = 89/155 (57.42%), Query Frame = 0


Query: 283 GNSSNWNEQLSSPEYEGSTPGHIRRNRRFYSI--TNYAQSELLSYVGDSGDYLDRQGFEE 342
           G      E++ + E +G+T     R RR Y     + + S L        + L   GFE 
Sbjct: 114 GGGGGAGERVENEEGDGAT-----RERRAYEFYYDDGSGSGLRPLPDSVSEILMGSGFER 173

Query: 343 LLEQIAETTTS----SRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVE 402
           LLEQ+++   S     R G PPA+ S +++LPR+ IS  H K ++ +CA+C +    G+E
Sbjct: 174 LLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEA-NCAVCTEVFEAGIE 233

Query: 403 VNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTD 432
             ++PC H++H  CI+PWLS RNSCP+CR+ELP+D
Sbjct: 234 GREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-22
Identity = 53/117 (45.30%), Postives = 69/117 (58.97%), Query Frame = 0


Query: 328 DSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           ++GDY    G EEL+EQ++  T    RG PPA  S +  LP + I+++HLK     C +C
Sbjct: 132 NAGDYFFGPGLEELIEQLSSGT--HHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVC 191

Query: 388 KDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSIN 445
           KD   L  E  Q+PC H+YH  CI+PWL   NSCP+CR ELP+  R      QSS N
Sbjct: 192 KDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS--RGSSSSTQSSQN 244

[Close]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL
Match: A0A0A0L2Z4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 PE=4 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 560/563 (99.47%), Postives = 561/563 (99.64%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQSNVHVIMNNNQ VDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240
           GPYRHSYGDSD+VSNRTYGGESD SLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240

Query: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480
           CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480

Query: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540
           QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ
Sbjct: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540

Query: 541 LDAHSAESSNQRENRSRRWWQIV 564
           LDAHSAESSNQRENRSRRWWQIV
Sbjct: 541 LDAHSAESSNQRENRSRRWWQIV 563

[Close]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL
Match: A0A5D3C795 (E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold287G00690 PE=4 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.1e-301
Identity = 523/560 (93.39%), Postives = 539/560 (96.25%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQS VHVIMN+NQ VDEHSPIQLPTCAFCQRVL  DSDA SEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR MRSSSESPENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNA+DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240
           GPYRHSYGDSD+VSNRTYGG+SDASL DGHSFLDTD+FILPGEGSDTDTDIDPMRAG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASL-DGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQE+GSSEARPQLQN +VSSASRRGNSSNW EQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFE+LLEQIAE TTSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAE-TTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHP+CILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480
           CPLCRYELPTDDRDYEEVK+SSIN AA H LQP VVGLENPSGLNGVGAN EFE+SQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540
            RNIDSAVISSNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GGQLTDGRQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHSAESSNQRENRSRRWW 561
           LDA SAESSNQRENR+RRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL
Match: A0A1S3CIY7 (uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=4 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.1e-301
Identity = 523/560 (93.39%), Postives = 539/560 (96.25%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQS VHVIMN+NQ VDEHSPIQLPTCAFCQRVL  DSDA SEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR MRSSSESPENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNA+DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240
           GPYRHSYGDSD+VSNRTYGG+SDASL DGHSFLDTD+FILPGEGSDTDTDIDPMRAG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASL-DGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQE+GSSEARPQLQN +VSSASRRGNSSNW EQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFE+LLEQIAE TTSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAE-TTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHP+CILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480
           CPLCRYELPTDDRDYEEVK+SSIN AA H LQP VVGLENPSGLNGVGAN EFE+SQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540
            RNIDSAVISSNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GGQLTDGRQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHSAESSNQRENRSRRWW 561
           LDA SAESSNQRENR+RRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL
Match: A0A6J1GDT0 (uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 3.3e-237
Identity = 432/562 (76.87%), Postives = 464/562 (82.56%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDID QRILEEFP S   +IM+NNQ V+E  P+Q  TCAFC+ VL PD+DA +E E I I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR    SSES ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           S H DRY DGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240
           GPYRHSYGDSD+VS RTYGG+SDASL DGHSFLDTD F+LP EGS  DTDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASL-DGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300
            HWDSSDEEDD GE TEA+TE +         LQ+FHVSSA+ RGN  NWNE+LSS EYE
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYE 300

Query: 301 GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPP 360
           GS    IRRN RFYSITNYA SE +SYVGDSGDYLDRQ FEELLEQIAE TTSSRRGAPP
Sbjct: 301 GSAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAE-TTSSRRGAPP 360

Query: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSAR 420
           AA+SFVKNL RLVISKEHLKHDSISCAICKDF  +GVEVNQLPCLHLYHP CILPWL+AR
Sbjct: 361 AAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTAR 420

Query: 421 NSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQE 480
           NSCPLCRYELPTDDRDYEE KQSS NAA +  LQ +V  LE+ S LN VGANPEF+TSQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQG 480

Query: 481 EDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDG 540
           E+  NID AV +S NSGRG RR WLFVAAP+VGLVGIALM W GNP      SGG++TD 
Sbjct: 481 EELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLVGIALMFWVGNP------SGGRITDR 540

Query: 541 RQLDAHSAESSNQRENRSRRWW 561
           RQLD   A+SSNQRENR+RRWW
Sbjct: 541 RQLDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL
Match: A0A6J1GEL6 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.0e-235
Identity = 434/587 (73.94%), Postives = 467/587 (79.56%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDID QRILEEFP S   +IM+NNQ V+E  P+Q  TCAFC+ VL PD+DA +E E I I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR    SSES ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           S H DRY DGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240
           GPYRHSYGDSD+VS RTYGG+SDASL DGHSFLDTD F+LP EGS  DTDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASL-DGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300
            HWDSSDEEDD GE TEA+TE +         LQ+FHVSSA+ RGN  NWNE+LSS EYE
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYE 300

Query: 301 -------------------------GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYL 360
                                    GS+PG IRRN RFYSITNYA SE +SYVGDSGDYL
Sbjct: 301 GSARDSITNNAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAHSESVSYVGDSGDYL 360

Query: 361 DRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHL 420
           DRQ FEELLEQIAE TTSSRRGAPPAA+SFVKNL RLVISKEHLKHDSISCAICKDF  +
Sbjct: 361 DRQSFEELLEQIAE-TTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLV 420

Query: 421 GVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQP 480
           GVEVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEE KQSS NAA +  LQ 
Sbjct: 421 GVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQH 480

Query: 481 RVVGLENPSGLNGVGANPEFETSQEEDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLV 540
           +V  LE+ S LN VGANPEF+TSQ E+  NID AV +S NSGRG RR WLFVAAP+VGLV
Sbjct: 481 QVTRLESRSDLNEVGANPEFDTSQGEELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLV 540

Query: 541 GIALMLWFGNPLCDQRVSGGQLTDGRQLDAHSAESSNQRENRSRRWW 561
           GIALM W GNP      SGG++TD RQLD   A+SSNQRENR+RRWW
Sbjct: 541 GIALMFWVGNP------SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

[Close]

BLAST of CSPI04G16850 vs. NCBI nr
Match: XP_004142724.1 (E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical protein Csa_012302 [Cucumis sativus])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 560/563 (99.47%), Postives = 561/563 (99.64%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQSNVHVIMNNNQ VDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240
           GPYRHSYGDSD+VSNRTYGGESD SLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240

Query: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480
           CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480

Query: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540
           QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ
Sbjct: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540

Query: 541 LDAHSAESSNQRENRSRRWWQIV 564
           LDAHSAESSNQRENRSRRWWQIV
Sbjct: 541 LDAHSAESSNQRENRSRRWWQIV 563

[Close]

BLAST of CSPI04G16850 vs. NCBI nr
Match: XP_008463301.1 (PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ubiquitin-protein ligase Praja-2 [Cucumis melo var. makuwa])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.3e-301
Identity = 523/560 (93.39%), Postives = 539/560 (96.25%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQS VHVIMN+NQ VDEHSPIQLPTCAFCQRVL  DSDA SEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR MRSSSESPENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNA+DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240
           GPYRHSYGDSD+VSNRTYGG+SDASL DGHSFLDTD+FILPGEGSDTDTDIDPMRAG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASL-DGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGN 240

Query: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQE+GSSEARPQLQN +VSSASRRGNSSNW EQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFE+LLEQIAE TTSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAE-TTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHP+CILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480
           CPLCRYELPTDDRDYEEVK+SSIN AA H LQP VVGLENPSGLNGVGAN EFE+SQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540
            RNIDSAVISSNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GGQLTDGRQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHSAESSNQRENRSRRWW 561
           LDA SAESSNQRENR+RRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of CSPI04G16850 vs. NCBI nr
Match: XP_038882118.1 (E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida])

HSP 1 Score: 979.5 bits (2531), Expect = 1.2e-281
Identity = 493/562 (87.72%), Postives = 515/562 (91.64%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDIDLQRILEEFPQS  HVIMN+NQ VDEHSPIQLP+CAFCQRVLVPD+DA  EPE ID+
Sbjct: 1   MDIDLQRILEEFPQSTAHVIMNSNQRVDEHSPIQLPSCAFCQRVLVPDNDATGEPEAIDV 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLED+ETPVRDSYHR T RGRR    S ES ENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDVETPVRDSYHRTTSRGRRTRYGSVESAENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           SSHGDRYADGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240
           GPYRHSYGDSD VS RTYGG+SDASL DGHSFLDTD+F+LPGEGS  DTDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDAVSYRTYGGDSDASL-DGHSFLDTDIFVLPGEGSDVDTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300
           GHWDSSDEEDDHGEFTEA+TEQEV SSEARPQLQNFHVSSA  RG SSNWNEQ SSPEYE
Sbjct: 241 GHWDSSDEEDDHGEFTEADTEQEVESSEARPQLQNFHVSSAPGRGISSNWNEQFSSPEYE 300

Query: 301 GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPP 360
           GS PG IRRNRRFYSITNYAQSELLSY+GDSG+YLDRQ FEELLEQIAE TTSSRRGAPP
Sbjct: 301 GSAPGQIRRNRRFYSITNYAQSELLSYIGDSGEYLDRQVFEELLEQIAE-TTSSRRGAPP 360

Query: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSAR 420
           AA+S VKNLPRLVISKEHLKHD+ISCAICKDFL +GVEVNQLPCLHLYHPSCILPWLSAR
Sbjct: 361 AAISIVKNLPRLVISKEHLKHDTISCAICKDFLLVGVEVNQLPCLHLYHPSCILPWLSAR 420

Query: 421 NSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQE 480
           NSCPLCRYELPTDDRDYEEVK+SSIN A +HGLQPRVV LENP  LN VGA+P FETSQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKRSSINTAEIHGLQPRVVSLENPRDLN-VGASPAFETSQG 480

Query: 481 EDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDG 540
           EDQ NIDSAV +SNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQ TD 
Sbjct: 481 EDQTNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQFTDR 540

Query: 541 RQLDAHSAESSNQRENRSRRWW 561
           RQL+A  A+SSNQRENR+RRWW
Sbjct: 541 RQLNARIADSSNQRENRTRRWW 559

[Close]

BLAST of CSPI04G16850 vs. NCBI nr
Match: KAG7033695.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.0 bits (2148), Expect = 3.0e-237
Identity = 438/587 (74.62%), Postives = 469/587 (79.90%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDID QRILEEFPQS  H+IM+NNQ V+E  P+Q  TC FC+ VL PD+DA +E E I I
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCVFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR    SSES ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           S HGDRY DGSNA DFMQHSSSH TPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHMTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240
           GPYRHSYGDSD+VS RTYGG+SDASL DGHSFLDTD FILP EGS  DTDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASL-DGHSFLDTDNFILPDEGSGVDTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300
            HWDSSDEEDD GE TEA+TE +         LQ+FHVSSAS RGN  NWNE+LS  EYE
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSASGRGNYGNWNEELSLSEYE 300

Query: 301 -------------------------GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYL 360
                                    GS+PG IRRN RFYSITNYAQSE +SYVGDSGDYL
Sbjct: 301 GSARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYL 360

Query: 361 DRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHL 420
           DRQ FEELLEQIAE TTSSRRGAPPAA+SFVKNL RLVISKEHLKHD ISCAICKDF  +
Sbjct: 361 DRQSFEELLEQIAE-TTSSRRGAPPAAISFVKNLSRLVISKEHLKHDGISCAICKDFFLV 420

Query: 421 GVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQP 480
           GVEVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEE KQSS NAA +  LQ 
Sbjct: 421 GVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQH 480

Query: 481 RVVGLENPSGLNGVGANPEFETSQEEDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLV 540
           +V  LE+ S LNGVGANPEF+TSQ E+  NID AV +S NSGRG RR WLFVAAP+VGLV
Sbjct: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLV 540

Query: 541 GIALMLWFGNPLCDQRVSGGQLTDGRQLDAHSAESSNQRENRSRRWW 561
           GIALM W GNP      SGG++TD RQLD  SA+SSNQRENR+RRWW
Sbjct: 541 GIALMFWVGNP------SGGRITDRRQLDTRSADSSNQRENRTRRWW 570

[Close]

BLAST of CSPI04G16850 vs. NCBI nr
Match: XP_022950071.1 (uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata])

HSP 1 Score: 830.9 bits (2145), Expect = 6.7e-237
Identity = 432/562 (76.87%), Postives = 464/562 (82.56%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSNVHVIMNNNQSVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60
           MDID QRILEEFP S   +IM+NNQ V+E  P+Q  TCAFC+ VL PD+DA +E E I I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR    SSES ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180
           S H DRY DGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDIVSNRTYGGESDASLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240
           GPYRHSYGDSD+VS RTYGG+SDASL DGHSFLDTD F+LP EGS  DTDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASL-DGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300
            HWDSSDEEDD GE TEA+TE +         LQ+FHVSSA+ RGN  NWNE+LSS EYE
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYE 300

Query: 301 GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPP 360
           GS    IRRN RFYSITNYA SE +SYVGDSGDYLDRQ FEELLEQIAE TTSSRRGAPP
Sbjct: 301 GSAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAE-TTSSRRGAPP 360

Query: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSAR 420
           AA+SFVKNL RLVISKEHLKHDSISCAICKDF  +GVEVNQLPCLHLYHP CILPWL+AR
Sbjct: 361 AAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTAR 420

Query: 421 NSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQE 480
           NSCPLCRYELPTDDRDYEE KQSS NAA +  LQ +V  LE+ S LN VGANPEF+TSQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQG 480

Query: 481 EDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDG 540
           E+  NID AV +S NSGRG RR WLFVAAP+VGLVGIALM W GNP      SGG++TD 
Sbjct: 481 EELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLVGIALMFWVGNP------SGGRITDR 540

Query: 541 RQLDAHSAESSNQRENRSRRWW 561
           RQLD   A+SSNQRENR+RRWW
Sbjct: 541 RQLDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of CSPI04G16850 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 4.4e-85
Identity = 220/537 (40.97%), Postives = 291/537 (54.19%), Query Frame = 0


Query: 38  CAFCQRVLVPDSDAPSEPEPIDICGDCKFLLLEDIETPVRDSYHRMTPRGRRAMR----- 97
           C  C RV+   SD   + E   +C DCKFLLLED  TP      R T R RR  R     
Sbjct: 24  CNLCGRVVQSASD---DLEIFSLCVDCKFLLLEDFGTPAPPLARRQTIRRRRMRRRRRTT 83

Query: 98  ----SSSESPENLFSQEFTHMINMVRQRQSSISSHGDRYADGSNAMDFMQHSSSHTTPNQ 157
               S S    +L SQ+FTH+I++ RQ  S++ +  D   D          +SSHTTP+ 
Sbjct: 84  RHDSSESVGVGDLSSQQFTHLISIARQSLSTVQASEDLRDD--------LRTSSHTTPSG 143

Query: 158 SRRWRQVYSDSENDGLDSFDSMFEESESNFSFGPYRHSYGDSDIVSNRTYGGESDASLDD 217
           S RWR ++S+SE+D  D+F     E+ESN SF  YR  + D+D +S   YGGESDAS D 
Sbjct: 144 STRWR-LFSESESDDFDNFG----ETESNASFSLYRFPHADNDTISFSAYGGESDASTD- 203

Query: 218 GHSFLDTDVFILPGEGSDT--DTDIDPMRAGVGHWDSSDEEDDHGEFTEAETEQEVGSSE 277
                  D+F+ P + SD   DTDIDPMRAG+  W +SDEED        E E+  G S 
Sbjct: 204 ----RHADIFVQPDDRSDIDFDTDIDPMRAGLNQW-NSDEED-------REWEEGAGPSG 263

Query: 278 ARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGSTPGHIRRNRRFYS---ITNYAQSELL 337
                   +++S S   +S     +  SPE E S    I   R   S    T     +  
Sbjct: 264 VAGTRYRNYLASPSESYSSMT---RFDSPELERSFRQRIIERRHSLSRNIFTGLEDLDFS 323

Query: 338 SYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSIS 397
            Y  + GDYLD +GF+ELLEQ+AE + +SRRGAPPA+VS V+NLPR++I++EH+    + 
Sbjct: 324 PYAANVGDYLDERGFDELLEQLAE-SDNSRRGAPPASVSCVRNLPRVIIAEEHVM-KGLV 383

Query: 398 CAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSI 457
           CAICK+   L  E  QLPCLHLYH  CI+PWLSARNSCPLCRYELPTDD+DYEE K++ +
Sbjct: 384 CAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEEGKRNVL 443

Query: 458 NAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEEDQRNIDSAVISSNNSGRGARRRWL 517
           +           V  ++ S  +G  +  E    + E +  +           R +R RWL
Sbjct: 444 D-----------VSEDSSSSDDGTESGEEEYVERGESESGV----------SRVSRGRWL 489

Query: 518 FV-AAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQLDAHSAESSNQRENRSRRW 560
           F+ AAP+V LVG+ L +W  +P              R +    A S +QRENR+RRW
Sbjct: 504 FLAAAPVVSLVGVVLAMWLSSP------------HRRGI----AISHSQRENRTRRW 489

[Close]

BLAST of CSPI04G16850 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 6.5e-28
Identity = 54/116 (46.55%), Postives = 75/116 (64.66%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G+ GDY    G E+L++Q+AE    +R G PPA+ S +  LP + ++K+ LK +   CA+
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAE-NDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAV 219

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSS 443
           C D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   Q S
Sbjct: 220 CMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGS 274

[Close]

BLAST of CSPI04G16850 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 116.7 bits (291), Expect = 6.0e-26
Identity = 58/140 (41.43%), Postives = 78/140 (55.71%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G++GDY    G EEL EQ++  TT  RRG PPA  S +  LP + I++ HL+    +C +
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT--RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPV 194

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELP------------TDDRD 446
           CKD   LG E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+
Sbjct: 195 CKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRN 254

Query: 447 YEEVKQSSINAAAVHGLQPR 455
           Y     SS + +  +G + R
Sbjct: 255 YRSSSSSSSSNSRENGNERR 272

[Close]

BLAST of CSPI04G16850 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 116.7 bits (291), Expect = 6.0e-26
Identity = 58/140 (41.43%), Postives = 78/140 (55.71%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G++GDY    G EEL EQ++  TT  RRG PPA  S +  LP + I++ HL+    +C +
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT--RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPV 194

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELP------------TDDRD 446
           CKD   LG E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+
Sbjct: 195 CKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRN 254

Query: 447 YEEVKQSSINAAAVHGLQPR 455
           Y     SS + +  +G + R
Sbjct: 255 YRSSSSSSSSNSRENGNERR 272

[Close]

BLAST of CSPI04G16850 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 116.7 bits (291), Expect = 6.0e-26
Identity = 58/140 (41.43%), Postives = 78/140 (55.71%), Query Frame = 0


Query: 327 GDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAI 386
           G++GDY    G EEL EQ++  TT  RRG PPA  S +  LP + I++ HL+    +C +
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT--RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPV 194

Query: 387 CKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELP------------TDDRD 446
           CKD   LG E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+
Sbjct: 195 CKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRN 254

Query: 447 YEEVKQSSINAAAVHGLQPR 455
           Y     SS + +  +G + R
Sbjct: 255 YRSSSSSSSSNSRENGNERR 272

The following BLAST results are available for this feature:

BLAST of CSPI04G16850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	9.1e-27	46.55	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	7.7e-26	49.53	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	8.5e-25	41.43	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SNB6	1.4e-22	38.06	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q94AK4	2.3e-22	45.30	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of CSPI04G16850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2Z4	0.0e+00	99.47	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 P...	[more]
A0A5D3C795	1.1e-301	93.39	E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3CIY7	1.1e-301	93.39	uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=...	[more]
A0A6J1GDT0	3.3e-237	76.87	uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GEL6	4.0e-235	73.94	E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=366...	[more]

BLAST of CSPI04G16850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142724.1	0.0e+00	99.47	E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical p...	[more]
XP_008463301.1	2.3e-301	93.39	PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ub...	[more]
XP_038882118.1	1.2e-281	87.72	E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiqu...	[more]
KAG7033695.1	3.0e-237	74.62	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022950071.1	6.7e-237	76.87	uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata]	[more]

BLAST of CSPI04G16850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01980.1	4.4e-85	40.97	RING/U-box superfamily protein	[more]
AT3G19950.1	6.5e-28	46.55	RING/U-box superfamily protein	[more]
AT2G40830.2	6.0e-26	41.43	RING-H2 finger C1A	[more]
AT2G40830.1	6.0e-26	41.43	RING-H2 finger C1A	[more]
AT2G40830.3	6.0e-26	41.43	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 384..424 e-value: 2.1E-5 score: 34.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 384..425 score: 11.795887
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 384..424 e-value: 4.3E-9 score: 36.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 258..298
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 134..152
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 119..152
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..247
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 223..304
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 322..484
None	No IPR available	PANTHER	PTHR15710:SF41	OS06G0101300 PROTEIN	coord: 322..484
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 365..431
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 345..431 e-value: 3.9E-17 score: 63.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G16850.1	CSPI04G16850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity