CSPI04G16520 (gene) Cucumber (PI 183967) v1

BLAST of CSPI04G16520 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.9e-198
Identity = 337/578 (58.30%), Postives = 427/578 (73.88%), Query Frame = 0

Query: 10  TASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVLHNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATKSLSTD 129
            +   G +   +CSF EMA+ FN + G+    P G FN++F +TG  Q DA++TKSL+  
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  L L   ++RAVP++WDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+ FL++QI R WAP    +   QRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGSRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV++VTGAQLGVWDFGS+NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQG-NNKLAKLVDTT 549
           +S++PGCTIRR++WDH P       + SS        ++SS T+   G + KLAK+VD++
Sbjct: 500 FSKVPGCTIRRSVWDHTP-------VASSGRLEPGGPSTSSSTEEVSGQSGKLAKIVDSS 559

Query: 550 EMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 EMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of CSPI04G16520 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.1e-118
Identity = 254/607 (41.85%), Postives = 364/607 (59.97%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN++F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP-GRQRKEPV 366
           IT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAP+  ++P G QRK+  
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 366

Query: 367 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYW 426
           C SLQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     
Sbjct: 367 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 426

Query: 427 DAHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLG 486
           D + +I        E  D R++E V WKN+SHV T P+E+ +    +LS   VVTGAQL 
Sbjct: 427 DLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLH 486

Query: 487 VWDFGSRNVLYMKLLYSRLPGCT-IRRTLWDHLPPIEKSKKLVS---------------- 546
           V   G +NVL+++L +SR+ G T ++ + WD          L+S                
Sbjct: 487 VESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPR 546

Query: 547 -SSDNTNNADNSSSDTKNTQGNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIV 584
            +  N N+A         TQ   KL K VDT+EM++GPQ+SPG+W+V+GA+L VEKGKI 
Sbjct: 547 PADVNINSAIYPGGPPVPTQA-PKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of CSPI04G16520 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.2e-106
Identity = 232/599 (38.73%), Postives = 340/599 (56.76%), Query Frame = 0

Query: 11  ASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEVDDEHTRDICLYDDVVLHNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V++NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATKSLSTD 130
            +       S I SF++M++ FNQ   LS   PSG FN++F F+     DAS+ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  E  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP-GRQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAP++  +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMLPYWDAHVAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++  P + L + D +  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   +VT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPGCTIRRTLWDHLPPIEKSK---KLVSSSDNTNNADNSSSDTKNTQ---- 550
           L+++L +S       R++ WD+L    +      ++S+  +T  + N ++    ++    
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 554

Query: 551 -----------GNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLL 585
                         KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSLL
Sbjct: 555 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of CSPI04G16520 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 3.5e-98
Identity = 223/621 (35.91%), Postives = 333/621 (53.62%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVEVDDEHTRDICLYDDVVLHNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGF-TGLKQTDASATKSLST 133
            +       S I  F++M++YFNQ+  ++   PSG+FN+ FGF +G   TDA+  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQP 253
            V+Q +SS L    ++ ++ D+ +Q F      TGS  +  +         ++Q  +P+ 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF------TGSCLLSTRRLNKAYHHSHSQPKFPEA 251

Query: 254 TSA-----------PYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPI 313
            +              +  +  +TVI  +RGGD   ++H  W  TV   PD I   F PI
Sbjct: 252 FNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 311

Query: 314 TALLDGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP--GRQRKEPV 373
           T+LL  V G   L+ A+ LYL YKPPL +L+ FL+F  PR WAP+H+ +P          
Sbjct: 312 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 371

Query: 374 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMLPY 433
            P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +    
Sbjct: 372 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 431

Query: 434 WDAHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG------- 493
            D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S        
Sbjct: 432 TDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDV 491

Query: 494 VYVVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKK---------- 553
            ++VTGAQL V   GS++VL+++L Y+++    + +  W H  PI  S+K          
Sbjct: 492 AFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVH-GPIGTSQKSGIFSSMSMP 551

Query: 554 LVSSSDNTNNADNSSSDTKNTQG----------NNKLAKLVDTTEMSKGPQDSPGHWLVT 586
           L S S + N      ++     G          NNK+ K VD +++ +GPQ SPGHWLVT
Sbjct: 552 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVT 611

BLAST of CSPI04G16520 vs. ExPASy TrEMBL
Match: A0A0A0L2V7 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332620 PE=4 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 582/586 (99.32%), Postives = 584/586 (99.66%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
           HNV WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM+PYWDAH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of CSPI04G16520 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 572/586 (97.61%), Postives = 579/586 (98.81%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNS FGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLELKEN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAPIHDK+PGRQRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSS TK+TQGNNK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of CSPI04G16520 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 572/586 (97.61%), Postives = 579/586 (98.81%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNS FGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLELKEN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAPIHDK+PGRQRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSS TK+TQGNNK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of CSPI04G16520 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 533/586 (90.96%), Postives = 554/586 (94.54%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFNS FGF G KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+ENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWD HV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHL   +KSKKLVSSSDN +NAD+SSS ++N QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLN-DKSKKLVSSSDN-SNADDSSSGSRNIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of CSPI04G16520 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.4e-310
Identity = 530/587 (90.29%), Postives = 551/587 (93.87%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
            NVSWDIKNFQ+PIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFN+ FGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +A K+LSTDGFYIPLAKFQ T T L L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELR FLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTN-NADNSSSDTKNTQGNN 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP  +KSKKLVSSSDN+N  AD+SSS +KN QG N
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSKKLVSSSDNSNAAADDSSSGSKNIQG-N 540

Query: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of CSPI04G16520 vs. NCBI nr
Match: XP_011653720.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KGN54446.1 hypothetical protein Csa_011952 [Cucumis sativus])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 582/586 (99.32%), Postives = 584/586 (99.66%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
           HNV WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM+PYWDAH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of CSPI04G16520 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 572/586 (97.61%), Postives = 579/586 (98.81%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
           HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNS FGFTGL+QTDA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           SATKSLSTDGFYIPLAKFQFTKTPLELKEN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAPIHDK+PGRQRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSS TK+TQGNNK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of CSPI04G16520 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 540/586 (92.15%), Postives = 560/586 (95.56%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFD+RLLYCKGV+GSTIVE+DDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIY+KQHQSSPLSTME+KRYVQDIANQRFSDTQGQ GSGSIK KEKGL+GGLFNN
Sbjct: 181 IGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYL+YKPPLEELR FLEFQIPRVWAP  D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTIRR LWDHLP  +KSKKLV+SSDN++  D+SSS TK+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRMLWDHLPN-DKSKKLVTSSDNSSKVDDSSSGTKSIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAK+VDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of CSPI04G16520 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 533/586 (90.96%), Postives = 554/586 (94.54%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFNS FGF G KQ DA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+ENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELR FLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWD HV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHL   +KSKKLVSSSDN +NAD+SSS ++N QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLN-DKSKKLVSSSDN-SNADDSSSGSRNIQG-NK 540

Query: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of CSPI04G16520 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1073.2 bits (2774), Expect = 9.0e-310
Identity = 530/587 (90.29%), Postives = 551/587 (93.87%), Query Frame = 0

Query: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120
            NVSWDIKNFQ+PIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFN+ FGFTG KQ DA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +A K+LSTDGFYIPLAKFQ T T L L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELR FLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTN-NADNSSSDTKNTQGNN 540
           RNVLYMKLLYSRLPGCTIRRTLWDHLP  +KSKKLVSSSDN+N  AD+SSS +KN QG N
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPN-DKSKKLVSSSDNSNAAADDSSSGSKNIQG-N 540

Query: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of CSPI04G16520 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.8e-199
Identity = 337/578 (58.30%), Postives = 427/578 (73.88%), Query Frame = 0

Query: 10  TASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVLHNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATKSLSTD 129
            +   G +   +CSF EMA+ FN + G+    P G FN++F +TG  Q DA++TKSL+  
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  L L   ++RAVP++WDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+ FL++QI R WAP    +   QRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYWDAHVAIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGSRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV++VTGAQLGVWDFGS+NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQG-NNKLAKLVDTT 549
           +S++PGCTIRR++WDH P       + SS        ++SS T+   G + KLAK+VD++
Sbjct: 500 FSKVPGCTIRRSVWDHTP-------VASSGRLEPGGPSTSSSTEEVSGQSGKLAKIVDSS 559

Query: 550 EMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 587
           EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 EMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of CSPI04G16520 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 427.2 bits (1097), Expect = 2.2e-119
Identity = 254/607 (41.85%), Postives = 364/607 (59.97%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN++F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP-GRQRKEPV 366
           IT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAP+  ++P G QRK+  
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 366

Query: 367 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMLPYW 426
           C SLQFS  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     
Sbjct: 367 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 426

Query: 427 DAHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLG 486
           D + +I        E  D R++E V WKN+SHV T P+E+ +    +LS   VVTGAQL 
Sbjct: 427 DLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLH 486

Query: 487 VWDFGSRNVLYMKLLYSRLPGCT-IRRTLWDHLPPIEKSKKLVS---------------- 546
           V   G +NVL+++L +SR+ G T ++ + WD          L+S                
Sbjct: 487 VESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPR 546

Query: 547 -SSDNTNNADNSSSDTKNTQGNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIV 584
            +  N N+A         TQ   KL K VDT+EM++GPQ+SPG+W+V+GA+L VEKGKI 
Sbjct: 547 PADVNINSAIYPGGPPVPTQA-PKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of CSPI04G16520 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 388.7 bits (997), Expect = 8.6e-108
Identity = 232/599 (38.73%), Postives = 340/599 (56.76%), Query Frame = 0

Query: 11  ASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEVDDEHTRDICLYDDVVLHNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V++NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATKSLSTD 130
            +       S I SF++M++ FNQ   LS   PSG FN++F F+     DAS+ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  E  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP-GRQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAP++  +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMLPYWDAHVAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++  P + L + D +  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   +VT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPGCTIRRTLWDHLPPIEKSK---KLVSSSDNTNNADNSSSDTKNTQ---- 550
           L+++L +S       R++ WD+L    +      ++S+  +T  + N ++    ++    
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 554

Query: 551 -----------GNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLL 585
                         KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSLL
Sbjct: 555 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of CSPI04G16520 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.5e-99
Identity = 223/621 (35.91%), Postives = 333/621 (53.62%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVEVDDEHTRDICLYDDVVLHNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGF-TGLKQTDASATKSLST 133
            +       S I  F++M++YFNQ+  ++   PSG+FN+ FGF +G   TDA+  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNNQGVYPQP 253
            V+Q +SS L    ++ ++ D+ +Q F      TGS  +  +         ++Q  +P+ 
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF------TGSCLLSTRRLNKAYHHSHSQPKFPEA 251

Query: 254 TSA-----------PYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPI 313
            +              +  +  +TVI  +RGGD   ++H  W  TV   PD I   F PI
Sbjct: 252 FNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 311

Query: 314 TALLDGVAGKEHLTRAILLYLEYKPPLEELRCFLEFQIPRVWAPIHDKIP--GRQRKEPV 373
           T+LL  V G   L+ A+ LYL YKPPL +L+ FL+F  PR WAP+H+ +P          
Sbjct: 312 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 371

Query: 374 CPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMLPY 433
            P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +    
Sbjct: 372 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 431

Query: 434 WDAHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG------- 493
            D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S        
Sbjct: 432 TDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDV 491

Query: 494 VYVVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKK---------- 553
            ++VTGAQL V   GS++VL+++L Y+++    + +  W H  PI  S+K          
Sbjct: 492 AFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVH-GPIGTSQKSGIFSSMSMP 551

Query: 554 LVSSSDNTNNADNSSSDTKNTQG----------NNKLAKLVDTTEMSKGPQDSPGHWLVT 586
           L S S + N      ++     G          NNK+ K VD +++ +GPQ SPGHWLVT
Sbjct: 552 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVT 611

BLAST of CSPI04G16520 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 206.1 bits (523), Expect = 7.9e-53
Identity = 124/326 (38.04%), Postives = 189/326 (57.98%), Query Frame = 0

Query: 7   ALHTASNAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLHNVS 66
           A   A  A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 67  WDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDASATK 126
             IK  +       S I  F +MA+ FNQ+  L+   PSG FN++F F+   Q DA+ TK
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTK 126

Query: 127 SLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGK 186
           +L+ DG +I L      K+ + L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGK
Sbjct: 127 NLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGK 186

Query: 187 DVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLEGG 246
           DVIY KQ  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L   
Sbjct: 187 DVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFA 246

Query: 247 LFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFNP 306
             ++ G Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F P
Sbjct: 247 DTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 306

Query: 307 ITALLDGVAGKEHLTRAILLYLEYKP 322
           IT+LL+GV G   L+ AI LYL  KP
Sbjct: 307 ITSLLNGVPGSGFLSHAINLYLRCKP 323