CSPI04G16240 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G16240
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	macrophage migration inhibitory factor homolog
Location	Chr4: 13526656 .. 13527928 (-)
RNA-Seq Expression	CSPI04G16240
Synteny	CSPI04G16240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTTCTCTCCCAATACTCAAAAACTCCCTCCTCCCTCTTCTCTCATCTCATCGCCATGCCTTGCCTTTACATCTCCACCAATGTCAACTTGGCCGGAGTCGACTCCGCTCCCATCTTCTCCGCCACTACCACCGCCGTCTCCTCCATTATTGGCAAACCCGAAAATGTATAAACCTTTGCTTCTTAAAATACATACTTCTTTTCATTCTTTCTCTTGTCCTCTCATCCTAATGCATCCTACAGTCCACTTATCTTCTTCTACACTATTTAGTTGGTTAATTAAAGCTACATAAATCATTGTCTTTTTAGACAATTTTTTATAGACGAGAAGAAAAACTATTTATAAGATATAGCACAAAAAAAACTTATTTTTTTATAAAAATATTCCATTTTTTAAAAATATATTTTTCCTATTGTTGGATTTGTGCTTCGGAGCCAAACGCCTAATTATGGACAATGCAAAAGTTTAATTTAATGGAAACATTCCTAACCATTTCTTTTTTTGATAGAACGAAGTGAAATTTCACTTTTATTAACTCTATATCTTTATTTATGTAATCCATTAATTTCAAGTTTTGTACCTTCTGTAAAATCATTTTCCCTCCTGTTTATATTTTAGCGTACGTGTTCGAATTTTAGTTTGGTTGAGAATGATATAATATCAAATTAACTCTTATGTTTGAGTTCAAATTTTTTGATTAATTAATAATTTGAGATTTTGGTATAGAGCAAGTGGTCAAAGTCCTGCAGTTTTATTTTCCTTTCAATTAAAATTGATTCACTTATTTGGTTTTGTTCATGTTTCCAATACATAAGTTGGAGTTCAAGTGGTGATTTAAGAGTTTTTTAAAAAAATCTCAATTTACTGTGATCCATCGTTTTGAGTTTGATTAGTGATTTAGTGCTCTTTTCATTACTTATGCCTGTAATTACCTTTCTCCTTTCTTTCTTCGACCGTCAAGTTGACAGTTTTTTTTTTGCCAACAGTATGTGATGGTGTTGCTGAATGGGTCGGTGCCGATATCGTTTGGAGGGAATGGGGATCCAGCAGCGTTTGCAGAGGTGGTGTCCATGGGAGGAATAAACTCAGAAGTGAAGAGGAGGTTGATCTCAACCCTCGGAAGCATTTTGAATGAAAAGCTTTCAGTTCCACCGGCGAGATTCTTCCTTAAAGTCCACGACACGACGGCTGGTCGCCCCATTTCCAAATTATGACTCTACCCGGTGGCTGCCTTTTCCACCTCTTCCAACTTTCATATATATATATATATA

mRNA sequence

CTCTTCTCTCCCAATACTCAAAAACTCCCTCCTCCCTCTTCTCTCATCTCATCGCCATGCCTTGCCTTTACATCTCCACCAATGTCAACTTGGCCGGAGTCGACTCCGCTCCCATCTTCTCCGCCACTACCACCGCCGTCTCCTCCATTATTGGCAAACCCGAAAATTATGTGATGGTGTTGCTGAATGGGTCGGTGCCGATATCGTTTGGAGGGAATGGGGATCCAGCAGCGTTTGCAGAGGTGGTGTCCATGGGAGGAATAAACTCAGAAGTGAAGAGGAGGTTGATCTCAACCCTCGGAAGCATTTTGAATGAAAAGCTTTCAGTTCCACCGGCGAGATTCTTCCTTAAAGTCCACGACACGACGGCTGGTCGCCCCATTTCCAAATTATGACTCTACCCGGTGGCTGCCTTTTCCACCTCTTCCAACTTTCATATATATATATATATA

Coding sequence (CDS)

ATGCCTTGCCTTTACATCTCCACCAATGTCAACTTGGCCGGAGTCGACTCCGCTCCCATCTTCTCCGCCACTACCACCGCCGTCTCCTCCATTATTGGCAAACCCGAAAATTATGTGATGGTGTTGCTGAATGGGTCGGTGCCGATATCGTTTGGAGGGAATGGGGATCCAGCAGCGTTTGCAGAGGTGGTGTCCATGGGAGGAATAAACTCAGAAGTGAAGAGGAGGTTGATCTCAACCCTCGGAAGCATTTTGAATGAAAAGCTTTCAGTTCCACCGGCGAGATTCTTCCTTAAAGTCCACGACACGACGGCTGGTCGCCCCATTTCCAAATTATGA

Protein sequence

MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAFAEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL*

Homology

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BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot
Match: P81748 (Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 4.5e-10
Identity = 35/102 (34.31%), Postives = 55/102 (53.92%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP    STNV    + S     +T+  ++ ++GKPE+YV V +NG   I+FGG   PA F
Sbjct: 1   MPIFTFSTNVPSENI-SVDFLKSTSKLIAGMLGKPESYVAVHINGGQKITFGGTDAPAGF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHD 103
            +++S+GG+  E  R   + L   L + L +P  R ++   D
Sbjct: 61  GQLLSLGGVGGEKNRSHSAKLFKHLTDGLGIPGNRMYINFVD 101

[Close]

BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot
Match: P81529 (Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 2.2e-09
Identity = 36/98 (36.73%), Postives = 55/98 (56.12%), Query Frame = 0


Query: 1  MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
          MP   ++TN+    V S    S+T+  V +I+ KP +YV V +N    +SFGG+  PAAF
Sbjct: 1  MPIFTLNTNIKATDVPS-DFLSSTSALVGNILSKPGSYVAVHINTDQQLSFGGSTKPAAF 60

Query: 61 AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFL 99
            ++S+GGI     R   + L   LN+KL +P  R ++
Sbjct: 61 GTLMSIGGIEPSRNRDHSAKLFDHLNKKLGIPKNRMYI 97

[Close]

BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot
Match: P91850 (Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_28435 PE=3 SV=4)

HSP 1 Score: 56.6 bits (135), Expect = 2.1e-07
Identity = 32/105 (30.48%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP   I TN+    + SA      +  V+  +GKPE+YV + +NG   + FGG+ DP A 
Sbjct: 1   MPYFTIDTNIPQNSISSA-FLKKASNVVAKALGKPESYVSIHVNGGQAMVFGGSEDPCAV 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTA 106
             + S+G +  +V       L  +L ++L +P  R +++  D  A
Sbjct: 61  CVLKSIGCVGPKVNNSHAEKLYKLLADELKIPKNRCYIEFVDIEA 104

[Close]

BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot
Match: O44786 (Macrophage migration inhibitory factor homolog OS=Wuchereria bancrofti OX=6293 GN=MIF PE=3 SV=3)

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 32/105 (30.48%), Postives = 50/105 (47.62%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP   I TN     + SA         V   +GKPE+YV + +NG  P+ FGG+ DP   
Sbjct: 1   MPYFTIDTNKPQDSISSA-FLKKAPNVVPKALGKPESYVSIHVNGGQPMVFGGSEDPCPV 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTA 106
             + S+G +  +V       L  +L ++L +P  R +++  D  A
Sbjct: 61  CVLKSIGCVGPKVNNSHAEKLYKLLADELKIPKNRCYIESVDIEA 104

[Close]

BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot
Match: P80177 (Macrophage migration inhibitory factor OS=Bos taurus OX=9913 GN=MIF PE=1 SV=6)

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-06
Identity = 31/105 (29.52%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP   ++TNV  A V    + S  T  ++   GKP  Y+ V +     ++FGG+ +P A 
Sbjct: 1   MPMFVVNTNVPRASVPDG-LLSELTQQLAQATGKPAQYIAVHVVPDQLMTFGGSSEPCAL 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTA 106
             + S+G I     R     L  +L E+L + P R ++   D  A
Sbjct: 61  CSLHSIGKIGGAQNRSYSKLLCGLLTERLRISPDRIYINFCDMNA 104

[Close]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL
Match: A0A0A0L1F7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314440 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 6.9e-54
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF
Sbjct: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL
Match: A0A1S3CL09 (macrophage migration inhibitory factor homolog OS=Cucumis melo OX=3656 GN=LOC103502168 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.7e-52
Identity = 110/112 (98.21%), Postives = 110/112 (98.21%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNVNLAGVDSA IFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPA F
Sbjct: 1   MPCLYISTNVNLAGVDSAAIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAVF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL
Match: A0A6J1GDU8 (macrophage migration inhibitory factor homolog OS=Cucurbita moschata OX=3662 GN=LOC111453099 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-46
Identity = 99/112 (88.39%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNV+LAG DSA IF+ATT+AVSSIIGKPENYVMVLL GSV ISFGGN +PAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLI+T+GSIL EKLSVPPARFFLKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFFLKVHDTTAGRPVSKL 112

[Close]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL
Match: A0A6J1CQN7 (macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN=LOC111013868 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.8e-46
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNV+LAG D+A IFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PAAF
Sbjct: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL
Match: A0A6J1IKS8 (macrophage migration inhibitory factor homolog OS=Cucurbita maxima OX=3661 GN=LOC111478343 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.9e-46
Identity = 98/112 (87.50%), Postives = 105/112 (93.75%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNV+LAG DSA IF+ATT+AVSSIIGKPENYVMVLL GSV ISFGGN +PAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLI+T+GSIL EKLSVPPARF LKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFVLKVHDTTAGRPVSKL 112

[Close]

BLAST of CSPI04G16240 vs. NCBI nr
Match: XP_004148599.1 (macrophage migration inhibitory factor homolog [Cucumis sativus])

HSP 1 Score: 219.5 bits (558), Expect = 1.4e-53
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF
Sbjct: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. NCBI nr
Match: XP_008464238.1 (PREDICTED: macrophage migration inhibitory factor homolog [Cucumis melo])

HSP 1 Score: 214.9 bits (546), Expect = 3.5e-52
Identity = 110/112 (98.21%), Postives = 110/112 (98.21%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNVNLAGVDSA IFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPA F
Sbjct: 1   MPCLYISTNVNLAGVDSAAIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAVF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. NCBI nr
Match: XP_038883562.1 (macrophage migration inhibitory factor homolog [Benincasa hispida])

HSP 1 Score: 209.5 bits (532), Expect = 1.5e-50
Identity = 107/112 (95.54%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNVNLAG DS  IFSATTTAVSSIIGKPENYVMVLL GSVPISFGGNG+PAAF
Sbjct: 1   MPCLYISTNVNLAGADSTAIFSATTTAVSSIIGKPENYVMVLLKGSVPISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. NCBI nr
Match: XP_022949815.1 (macrophage migration inhibitory factor homolog [Cucurbita moschata] >XP_023543101.1 macrophage migration inhibitory factor homolog [Cucurbita pepo subsp. pepo] >KAG6603558.1 hypothetical protein SDJN03_04167, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033748.1 hypothetical protein SDJN02_03473, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 195.7 bits (496), Expect = 2.2e-46
Identity = 99/112 (88.39%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNV+LAG DSA IF+ATT+AVSSIIGKPENYVMVLL GSV ISFGGN +PAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINSEVKRRLI+T+GSIL EKLSVPPARFFLKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFFLKVHDTTAGRPVSKL 112

[Close]

BLAST of CSPI04G16240 vs. NCBI nr
Match: XP_022144090.1 (macrophage migration inhibitory factor homolog [Momordica charantia])

HSP 1 Score: 194.9 bits (494), Expect = 3.8e-46
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYISTNV+LAG D+A IFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PAAF
Sbjct: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of CSPI04G16240 vs. TAIR 10
Match: AT5G01650.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.1e-30
Identity = 61/103 (59.22%), Postives = 83/103 (80.58%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCL +STNVNL GVD++ I S  ++ V+ IIGKPENYVM++L GSVP+SFGG  DPAA+
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDT 104
            E+VS+GG+N++V ++L + + +IL  KLSVP +RFFLK +DT
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDT 103

[Close]

BLAST of CSPI04G16240 vs. TAIR 10
Match: AT5G01650.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.1e-30
Identity = 61/103 (59.22%), Postives = 83/103 (80.58%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCL +STNVNL GVD++ I S  ++ V+ IIGKPENYVM++L GSVP+SFGG  DPAA+
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDT 104
            E+VS+GG+N++V ++L + + +IL  KLSVP +RFFLK +DT
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDT 103

[Close]

BLAST of CSPI04G16240 vs. TAIR 10
Match: AT3G51660.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.2e-29
Identity = 64/112 (57.14%), Postives = 82/112 (73.21%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MPCLYI+TNVN  GV++ P +S  T AV+SI+G+P+N VMV+L GSV I FGGN + AA+
Sbjct: 1   MPCLYITTNVNFDGVNTDPFYSEVTKAVASIVGRPQNLVMVVLKGSVEIVFGGNKEAAAY 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
           AE+VSMGGI  +VKR LI+T+GSIL+   S+ P RF  KV D  +    SKL
Sbjct: 61  AEIVSMGGITKQVKRELIATVGSILHTHFSIHPTRFIFKVFDINSLPLPSKL 112

[Close]

BLAST of CSPI04G16240 vs. TAIR 10
Match: AT5G57170.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 1.7e-20
Identity = 49/112 (43.75%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP L + TN+ +  V  + I    T AV+ IIGKPE+YVM+LLN  VPI+F G  +PAA+
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 113
            E++S+GG+   V  +L  T+  IL  KLS+  +RF++K +D+    P+S L
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDS----PVSPL 108

[Close]

BLAST of CSPI04G16240 vs. TAIR 10
Match: AT5G57170.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 3.8e-20
Identity = 46/103 (44.66%), Postives = 68/103 (66.02%), Query Frame = 0


Query: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60
           MP L + TN+ +  V  + I    T AV+ IIGKPE+YVM+LLN  VPI+F G  +PAA+
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDT 104
            E++S+GG+   V  +L  T+  IL  KLS+  +RF++K +D+
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDS 103

The following BLAST results are available for this feature:

BLAST of CSPI04G16240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P81748	4.5e-10	34.31	Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 P...	[more]
P81529	2.2e-09	36.73	Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 P...	[more]
P91850	2.1e-07	30.48	Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_2...	[more]
O44786	1.4e-06	30.48	Macrophage migration inhibitory factor homolog OS=Wuchereria bancrofti OX=6293 G...	[more]
P80177	6.7e-06	29.52	Macrophage migration inhibitory factor OS=Bos taurus OX=9913 GN=MIF PE=1 SV=6	[more]

BLAST of CSPI04G16240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1F7	6.9e-54	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314440 PE=3 SV=1	[more]
A0A1S3CL09	1.7e-52	98.21	macrophage migration inhibitory factor homolog OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1GDU8	1.1e-46	88.39	macrophage migration inhibitory factor homolog OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1CQN7	1.8e-46	87.50	macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1IKS8	6.9e-46	87.50	macrophage migration inhibitory factor homolog OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of CSPI04G16240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148599.1	1.4e-53	100.00	macrophage migration inhibitory factor homolog [Cucumis sativus]	[more]
XP_008464238.1	3.5e-52	98.21	PREDICTED: macrophage migration inhibitory factor homolog [Cucumis melo]	[more]
XP_038883562.1	1.5e-50	95.54	macrophage migration inhibitory factor homolog [Benincasa hispida]	[more]
XP_022949815.1	2.2e-46	88.39	macrophage migration inhibitory factor homolog [Cucurbita moschata] >XP_02354310...	[more]
XP_022144090.1	3.8e-46	87.50	macrophage migration inhibitory factor homolog [Momordica charantia]	[more]

BLAST of CSPI04G16240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01650.1	1.1e-30	59.22	Tautomerase/MIF superfamily protein	[more]
AT5G01650.2	1.1e-30	59.22	Tautomerase/MIF superfamily protein	[more]
AT3G51660.1	1.2e-29	57.14	Tautomerase/MIF superfamily protein	[more]
AT5G57170.2	1.7e-20	43.75	Tautomerase/MIF superfamily protein	[more]
AT5G57170.1	3.8e-20	44.66	Tautomerase/MIF superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001398	Macrophage migration inhibitory factor	PFAM	PF01187	MIF	coord: 2..104 e-value: 4.6E-13 score: 49.5
IPR001398	Macrophage migration inhibitory factor	PANTHER	PTHR11954	D-DOPACHROME DECARBOXYLASE	coord: 1..112
IPR014347	Tautomerase/MIF superfamily	GENE3D	3.30.429.10	Macrophage Migration Inhibitory Factor	coord: 2..107 e-value: 1.5E-32 score: 114.4
IPR014347	Tautomerase/MIF superfamily	SUPERFAMILY	55331	Tautomerase/MIF	coord: 1..104
None	No IPR available	PANTHER	PTHR11954:SF25	LS1-LIKE PROTEIN	coord: 1..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G16240.1	CSPI04G16240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0050178	phenylpyruvate tautomerase activity