CSPI04G16020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G16020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPyruvate decarboxylase
LocationChr4: 13347839 .. 13351785 (+)
RNA-Seq ExpressionCSPI04G16020
SyntenyCSPI04G16020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAATAATTGTAATATTTTTACCATGCACAAAGATAATAAATCAAACATTTATAATTTGAGATTAGATGGTATATTTAGCTTTCAATGGAGTTACCATTCTTTTAATCATGTGCATGTGTTTTTCAACATTCAATCACGGTATAAAATTGTGAATCTAACCGGTTTCCTCCACAAACTTGGATTGTCGTTGATATAAATAAGTTGGGCAGTACAAACTTTGTTATTCACCATTACACAATCCTTCCTACGCCCTACACAAGATTCATTCATCCATAAAAACTCAAAAGCTATGGCTACGAAGCTCGAAAATGGCTTCCATACTGAAACCAACCCAAAGGTCACAACGACAATGGAAGCATCCCACAGTCCTACAGTTAACCCACTAGCCAATGGTATAGCAGCTGCTGACAAGCTTCGTAGCCAATCTCGCAGCAGTCATAATAATGGCGCCATCACGCCCTCAACGGTCATAATCCCACCTCCCACAACTACCCCGTCCACGCTTGGGCACTACCTAGCCCGTCGTCTGGTTCAAATCGGTATCTCCGATATCTTTTCCGTTCCGGGAGACTCTAATTTGGTGCTGTTTGATTACTTTGTGGCAGCAAAAGGGTTGAACCTTGTGGGATGTTGTAGTGAGCTTAATGCTGGCTATGCGGCTGATGGGTATGCTAGGCGCCGTGGTGTTGGAGCATGTGCTGTAACCTTCACAGTTGGTAGTCTTAGTCTCATCAATGCTATTGCCGGTGCTTACTCGGAAGATCTTCCTGTGATTTGCATTGTGGGTGGACCAAACTCTAATGATTATGGAAGTAAGAAGATTCTCCATCATACCATTGGATTGCCGGATTTCGGTCAAGAACTTCGATGCTTTCAAAATGTCACTTGTTATCAGGTAGCTCCAAAATCTAAATATTTTCTCAAATATTGGAATATGATTTTTAGGGATTAAAAAGTGATCTTTTAGTTTATAGAGTCCGTGCGAAATTATTTTAAACAATCAGTACTTGACGTACTTTAGTTTAAGTTATCATATTCTTGGTTTAAGATCAATAGTAACTCAACCTACCATTCAACGTCAAGATATTGGAGCTCTATATTATGATGGGTCACACTCTCTATCTAGTCTGTCGCCTGATCCAGCTAGGTTCAAGCAACCCTCAGTAATTGTATGGGTAAAAAAACAAAAACAAAAGGTGCTCAATTTTTTTTTGGTTGTACAGAAACGTCATTGTTACATAAAATTAAAGAGAGAAGCAAATCTACAAAACAGAAAGCAGTTCTATAGCCAATAAATGTTAATATATATTCCTTTGATCTAAAAAATATCCATATATTCCCTCAATAACTTTGACAGGCAGCTCATAAATCTAAATACCTTTTTCGATATTGAAATATGAAATTAATTTTATGGGCTAAAAAGGTAACTTTAGTTTACAAAATAACTTTGAGTGAAATTATTTTAAACCGGCAATTCATTGTTTTGTTGGTCCACTGGAACTCTAAAATATTGTCTAACCACATTACATCAGAAAAGCAAATATGTCTTAGCTTGTTGTAGCGTATAAATATCCAATTATTCTTTGGAAGCTACATAACATTAAAAGGGTGTTAAATTAAGTTTAAGTTTGATTAAAGTAATTTATGAATGTGATTATAATCATCGTCGATTATTGTGTTTCTGTGTGTAGGCCATTATTGACAACTTGGAAGATGCACAATGGCAAATAGACACAGCAATTTCAAAATGCCTAGAAGAAAGCAAACCAATTTATATTAGCATTTGTTGCAATTTGGTGGCCATCCCTCATCCTTCTTTCTCTGCTCAACAACTCATCCCACTCTCACTCTCCTCCAAGTAATTCTAATTTCCCCTCTCAAATCTAACCTAATATTAATTAATATCTTTTCATCGTTTGAGCCTTTCAACTCTTGTTTTTATTTCTAGTCTCAAAACTTTAAAATAGTACAAATTTACTTTAGAGATTTCAAAATTTTCACTATTAAAAATGTTAAAAATTCAGTATTTTAATCCTTATTTTCTGGATGATCATTCACAGGGAAAGTAACCAAATGGCTTTAGAGGCAGCAGTAGAAAAAGCAGCTCAACTACTAAACACAGCTATAAAACCCGTCATGATCGGAGGCAGGAAACTCCGACTAACCAACTCACAAGAACCATTCATCGAACTAGCCAACGCTTGTGGGTACCCCGTCGCCGTGATGCCGTCGGCAAAAGGCATGTTCCCAGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAATCAGCTCCACCTATTGCGGGGAGACGGTAGAAATAGCGGACGCCTCAATCTTCGTGGGAGCCATTTTAGACGAACTAGATACCGTTGCCTTCTCACTTACCTACAAAAAGAACAAAGGCATTATGGTAGAAGCCGATCGAGTTGTGTTTCCAAATGGGCCGAGTTTGGGGCCAATATTAATGAAGGATTTTCTACGGGCTTTAGGGAAACGATTGAAGCCCAATTCAACGGCTTACGAGAATTATAGGCGGATTTATATAGCGGAGTCGGGTCCACTAGAATCGGGTCCGGGTGAAGCGTTACGGGTGAATATTGTGTTCAAACACATTCAGAAAATGTTATCGAGTAATATGGCTGTGATATCAGAGGCGGGTGATTCTTGGTTCCACAGTCAAAGATTAAAACTACCGAAATCATGCGGGTAAAACACATCTTTACCGATTACAATTATCATTTTGGTCCGAGTATATAATATGAAATGGTTTTGGATATATAACATGTAGGTATGAGGTACAACTCCTTTATGCATCTATAGGATGGTCTTTGGGTGCAACTCTTGGATATGCACAAGCTGCACCCGAAAAACGAGTGGTTCTTCTTATTGGTGATGGCAGCTTTCAGGTCCATTCTTGTTTATATATTCAATTATATTATTTAATACTATTTTCCTTAATTCATATCTTCTAGGTTAAATTATAAAAAAATTGTTCTTGAATTTATTTTAACTTTCAAAAGTTTTTAAAATACAATAAATAAATAAAAAGGGTTAAATATGAAAACTGTTATTATTTTTTTCCTTTAAACTTTTAAAAGTTGCATTTAGAATGCTTGCTTATCTAATTTTAAAATGCTTTTATGATTAATGTATATGTTTTATTTTAGATTTTTTTTAAGAAAAAAGAAAAAATAAAAGATATTTTTGTGTTAAGTTAAACGTTTGAAAATCATTTGAATGTTTAAAAAATAATTCAAACTAAGTTAAATGTAAAATTATTATTTTTGAAATAACTGGAATGATTTACATGACAGATGACTCCACAAGATGTGTCTACAATGATAACATTGAAGCAAAATAATATAATATTTTTAATAAATAATGGTGGATACACAATTGAAGTTGAAATTCATGATGGCCCCTACAATGTTATCAAGAATTGGGACTACACTGCCATTGTTAATGCTATGCACAATCATGATGGAAATTGTTGGACTACCAAGGTAAAATCTCCCTACTCTATTTGTATTTATTTTTTAACATGCGTACAAACATGAATTAATAGTTAAAGAGAGAAAAAGTGATATGGTACATTTTGTCACATTTTTCTAACTTAAAGTTTTGGTTGTCATGTAGGTTCATACTGAGGAAGAGTTGGTAAATGCAATCGAGATAGTATTGAAAGAAAGAAAAGATAGTTTGTGCTTCATAGAGGTTATTGTTCATAGAGATGATACTAGTAAAGAGTTATTAGAATTTGGAAGCAAGTTTGCTGCTGTTAGTAGTCGTCCCCCAAGAGTACTCCTATGAAATAGAAAATTGAAATAAAAAATTTGGTTCACATAGAATGAGACTCATGTTAGAATTTAGTTTGTACGATTTAAAAAGATAGATATCATATTCATATTCATATCTATATTATTATTATTATATATAAAAG

mRNA sequence

AAAAATAATTGTAATATTTTTACCATGCACAAAGATAATAAATCAAACATTTATAATTTGAGATTAGATGGTATATTTAGCTTTCAATGGAGTTACCATTCTTTTAATCATGTGCATGTGTTTTTCAACATTCAATCACGGTATAAAATTGTGAATCTAACCGGTTTCCTCCACAAACTTGGATTGTCGTTGATATAAATAAGTTGGGCAGTACAAACTTTGTTATTCACCATTACACAATCCTTCCTACGCCCTACACAAGATTCATTCATCCATAAAAACTCAAAAGCTATGGCTACGAAGCTCGAAAATGGCTTCCATACTGAAACCAACCCAAAGGTCACAACGACAATGGAAGCATCCCACAGTCCTACAGTTAACCCACTAGCCAATGGTATAGCAGCTGCTGACAAGCTTCGTAGCCAATCTCGCAGCAGTCATAATAATGGCGCCATCACGCCCTCAACGGTCATAATCCCACCTCCCACAACTACCCCGTCCACGCTTGGGCACTACCTAGCCCGTCGTCTGGTTCAAATCGGTATCTCCGATATCTTTTCCGTTCCGGGAGACTCTAATTTGGTGCTGTTTGATTACTTTGTGGCAGCAAAAGGGTTGAACCTTGTGGGATGTTGTAGTGAGCTTAATGCTGGCTATGCGGCTGATGGGTATGCTAGGCGCCGTGGTGTTGGAGCATGTGCTGTAACCTTCACAGTTGGTAGTCTTAGTCTCATCAATGCTATTGCCGGTGCTTACTCGGAAGATCTTCCTGTGATTTGCATTGTGGGTGGACCAAACTCTAATGATTATGGAAGTAAGAAGATTCTCCATCATACCATTGGATTGCCGGATTTCGGTCAAGAACTTCGATGCTTTCAAAATGTCACTTGTTATCAGGCCATTATTGACAACTTGGAAGATGCACAATGGCAAATAGACACAGCAATTTCAAAATGCCTAGAAGAAAGCAAACCAATTTATATTAGCATTTGTTGCAATTTGGTGGCCATCCCTCATCCTTCTTTCTCTGCTCAACAACTCATCCCACTCTCACTCTCCTCCAAGGAAAGTAACCAAATGGCTTTAGAGGCAGCAGTAGAAAAAGCAGCTCAACTACTAAACACAGCTATAAAACCCGTCATGATCGGAGGCAGGAAACTCCGACTAACCAACTCACAAGAACCATTCATCGAACTAGCCAACGCTTGTGGGTACCCCGTCGCCGTGATGCCGTCGGCAAAAGGCATGTTCCCAGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAATCAGCTCCACCTATTGCGGGGAGACGGTAGAAATAGCGGACGCCTCAATCTTCGTGGGAGCCATTTTAGACGAACTAGATACCGTTGCCTTCTCACTTACCTACAAAAAGAACAAAGGCATTATGGTAGAAGCCGATCGAGTTGTGTTTCCAAATGGGCCGAGTTTGGGGCCAATATTAATGAAGGATTTTCTACGGGCTTTAGGGAAACGATTGAAGCCCAATTCAACGGCTTACGAGAATTATAGGCGGATTTATATAGCGGAGTCGGGTCCACTAGAATCGGGTCCGGGTGAAGCGTTACGGGTGAATATTGTGTTCAAACACATTCAGAAAATGTTATCGAGTAATATGGCTGTGATATCAGAGGCGGGTGATTCTTGGTTCCACAGTCAAAGATTAAAACTACCGAAATCATGCGGGTATGAGGTACAACTCCTTTATGCATCTATAGGATGGTCTTTGGGTGCAACTCTTGGATATGCACAAGCTGCACCCGAAAAACGAGTGGTTCTTCTTATTGGTGATGGCAGCTTTCAGATGACTCCACAAGATGTGTCTACAATGATAACATTGAAGCAAAATAATATAATATTTTTAATAAATAATGGTGGATACACAATTGAAGTTGAAATTCATGATGGCCCCTACAATGTTATCAAGAATTGGGACTACACTGCCATTGTTAATGCTATGCACAATCATGATGGAAATTGTTGGACTACCAAGGTTCATACTGAGGAAGAGTTGGTAAATGCAATCGAGATAGTATTGAAAGAAAGAAAAGATAGTTTGTGCTTCATAGAGGTTATTGTTCATAGAGATGATACTAGTAAAGAGTTATTAGAATTTGGAAGCAAGTTTGCTGCTGTTAGTAGTCGTCCCCCAAGAGTACTCCTATGAAATAGAAAATTGAAATAAAAAATTTGGTTCACATAGAATGAGACTCATGTTAGAATTTAGTTTGTACGATTTAAAAAGATAGATATCATATTCATATTCATATCTATATTATTATTATTATATATAAAAG

Coding sequence (CDS)

ATGGCTACGAAGCTCGAAAATGGCTTCCATACTGAAACCAACCCAAAGGTCACAACGACAATGGAAGCATCCCACAGTCCTACAGTTAACCCACTAGCCAATGGTATAGCAGCTGCTGACAAGCTTCGTAGCCAATCTCGCAGCAGTCATAATAATGGCGCCATCACGCCCTCAACGGTCATAATCCCACCTCCCACAACTACCCCGTCCACGCTTGGGCACTACCTAGCCCGTCGTCTGGTTCAAATCGGTATCTCCGATATCTTTTCCGTTCCGGGAGACTCTAATTTGGTGCTGTTTGATTACTTTGTGGCAGCAAAAGGGTTGAACCTTGTGGGATGTTGTAGTGAGCTTAATGCTGGCTATGCGGCTGATGGGTATGCTAGGCGCCGTGGTGTTGGAGCATGTGCTGTAACCTTCACAGTTGGTAGTCTTAGTCTCATCAATGCTATTGCCGGTGCTTACTCGGAAGATCTTCCTGTGATTTGCATTGTGGGTGGACCAAACTCTAATGATTATGGAAGTAAGAAGATTCTCCATCATACCATTGGATTGCCGGATTTCGGTCAAGAACTTCGATGCTTTCAAAATGTCACTTGTTATCAGGCCATTATTGACAACTTGGAAGATGCACAATGGCAAATAGACACAGCAATTTCAAAATGCCTAGAAGAAAGCAAACCAATTTATATTAGCATTTGTTGCAATTTGGTGGCCATCCCTCATCCTTCTTTCTCTGCTCAACAACTCATCCCACTCTCACTCTCCTCCAAGGAAAGTAACCAAATGGCTTTAGAGGCAGCAGTAGAAAAAGCAGCTCAACTACTAAACACAGCTATAAAACCCGTCATGATCGGAGGCAGGAAACTCCGACTAACCAACTCACAAGAACCATTCATCGAACTAGCCAACGCTTGTGGGTACCCCGTCGCCGTGATGCCGTCGGCAAAAGGCATGTTCCCAGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAATCAGCTCCACCTATTGCGGGGAGACGGTAGAAATAGCGGACGCCTCAATCTTCGTGGGAGCCATTTTAGACGAACTAGATACCGTTGCCTTCTCACTTACCTACAAAAAGAACAAAGGCATTATGGTAGAAGCCGATCGAGTTGTGTTTCCAAATGGGCCGAGTTTGGGGCCAATATTAATGAAGGATTTTCTACGGGCTTTAGGGAAACGATTGAAGCCCAATTCAACGGCTTACGAGAATTATAGGCGGATTTATATAGCGGAGTCGGGTCCACTAGAATCGGGTCCGGGTGAAGCGTTACGGGTGAATATTGTGTTCAAACACATTCAGAAAATGTTATCGAGTAATATGGCTGTGATATCAGAGGCGGGTGATTCTTGGTTCCACAGTCAAAGATTAAAACTACCGAAATCATGCGGGTATGAGGTACAACTCCTTTATGCATCTATAGGATGGTCTTTGGGTGCAACTCTTGGATATGCACAAGCTGCACCCGAAAAACGAGTGGTTCTTCTTATTGGTGATGGCAGCTTTCAGATGACTCCACAAGATGTGTCTACAATGATAACATTGAAGCAAAATAATATAATATTTTTAATAAATAATGGTGGATACACAATTGAAGTTGAAATTCATGATGGCCCCTACAATGTTATCAAGAATTGGGACTACACTGCCATTGTTAATGCTATGCACAATCATGATGGAAATTGTTGGACTACCAAGGTTCATACTGAGGAAGAGTTGGTAAATGCAATCGAGATAGTATTGAAAGAAAGAAAAGATAGTTTGTGCTTCATAGAGGTTATTGTTCATAGAGATGATACTAGTAAAGAGTTATTAGAATTTGGAAGCAAGTTTGCTGCTGTTAGTAGTCGTCCCCCAAGAGTACTCCTATGA

Protein sequence

MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTVIIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPPRVLL*
Homology
BLAST of CSPI04G16020 vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 7.1e-230
Identity = 381/568 (67.08%), Postives = 453/568 (79.75%), Query Frame = 0

Query: 63  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 122
           P  TT+ STLG +L+RRLVQ G++D+FSVPGD NL L D+ +A   LN +GCC+ELNAGY
Sbjct: 34  PITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGY 93

Query: 123 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 182
           AADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LPVICIVGGPNSND+G+ +ILHHT
Sbjct: 94  AADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHT 153

Query: 183 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 242
           IGLPDF QELRCFQ VTCYQA+++NLEDA  QID AI+  L+ESKP+YISI CNL A PH
Sbjct: 154 IGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPH 213

Query: 243 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 302
           P+F A+  +P  L+ + SN M LEAAVE   + LN A+KPVM+GG KLR+  + E F+EL
Sbjct: 214 PTF-ARDPVPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLEL 273

Query: 303 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 362
           A+A GYP+AVMPS KG+ PENHPHFIGTYWG +S+ +C E VE ADA IF G I ++  +
Sbjct: 274 ADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSS 333

Query: 363 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 422
           V +SL  KK K I+V  DRVV  NGP+ G +LM DF R L KR+K N TAYENY RI++ 
Sbjct: 334 VGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVP 393

Query: 423 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 482
           E  PL+  PGE LRVN +F+HIQKMLSS  AVI+E GDSWF+ Q+LKLPK CGYE Q+ Y
Sbjct: 394 EGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQY 453

Query: 483 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 542
            SIGWS+GATLGYAQA PEKRV+  IGDGSFQ+T QD+STMI   Q  IIFLINNGGYTI
Sbjct: 454 GSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTI 513

Query: 543 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 602
           EVEIHDGPYNVIKNW+YT +V+A+HN +G CWTTKV  EEELV AI+    E+KDSLCFI
Sbjct: 514 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFI 573

Query: 603 EVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           EVIVH+DDTSKELLE+GS+ +A + RPP
Sbjct: 574 EVIVHKDDTSKELLEWGSRVSAANGRPP 600

BLAST of CSPI04G16020 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 3.1e-225
Identity = 379/603 (62.85%), Postives = 471/603 (78.11%), Query Frame = 0

Query: 30  NPLANGIAAADKLRSQSRSSHNNGAITPSTVIIPPPTTTP--STLGHYLARRLVQIGISD 89
           NP +NG+A        S +S  +  +  S  +I PP T+   +TLG +LARRLVQIG +D
Sbjct: 8   NP-SNGVA------KPSCNSVGSLPVVSSNAVIHPPVTSAAGATLGRHLARRLVQIGATD 67

Query: 90  IFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSL 149
           +F+VPGD NL L DY +A  GL L+GCC+ELNAGYAADGYAR RGVGACAVTFTVG LS+
Sbjct: 68  VFAVPGDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSV 127

Query: 150 INAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDN 209
           +NAIAGAYSE+LPVICIVGGPNSNDYG+ +ILHHTIGLPDF QELRCFQ +TCYQA+I+N
Sbjct: 128 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINN 187

Query: 210 LEDAQWQIDTAISKCLEESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEA 269
           L+DA  QIDTAI+  L ESKP+YIS+ CNL  + HP+FS ++ +PL +S + SN+  LE 
Sbjct: 188 LDDAHEQIDTAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEY 247

Query: 270 AVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHF 329
           AVE AA  LN A+KPVM+GG K+R+  +++ F  +A + GYP+AVMPSAKG+ PE+HP F
Sbjct: 248 AVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPIAVMPSAKGLVPEHHPRF 307

Query: 330 IGTYWGTISSTYCGETVEIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNG 389
           IGTYWG +S+T+C E VE ADA +F G I ++  +V +SL  K+ K ++V+ DRVV  NG
Sbjct: 308 IGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNG 367

Query: 390 PSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKM 449
           P+ G ILM +FL AL KRL  N+TAY+NYRRI+I +  P    P E LRVNI+FKHI++M
Sbjct: 368 PAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEM 427

Query: 450 LSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLL 509
           LS + AVI+E GDSWF+ Q+L+LP+ CGYE Q+ Y SIGWS+GATLGYAQAA +KRV+  
Sbjct: 428 LSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISC 487

Query: 510 IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMH 569
           IGDGSFQMT QDVSTM+   Q +IIFLINNGGYTIEVEIHDGPYNVIKNWDYT +++A+H
Sbjct: 488 IGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIH 547

Query: 570 NHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSS 629
           N DGNCWT KV TEEEL+ AI      +KD LCFIE+IVH+DDTSKELLE+GS+ +A +S
Sbjct: 548 NSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANS 602

Query: 630 RPP 631
           RPP
Sbjct: 608 RPP 602

BLAST of CSPI04G16020 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 9.0e-225
Identity = 379/603 (62.85%), Postives = 470/603 (77.94%), Query Frame = 0

Query: 30  NPLANGIAAADKLRSQSRSSHNNGAITPSTVIIPPPTTTP--STLGHYLARRLVQIGISD 89
           NP +NG+A        S +S  +  +  S  +I PP T+   +TLG +LARRLVQIG +D
Sbjct: 8   NP-SNGVA------KPSCNSVGSLPVVSSNAVINPPVTSAAGATLGRHLARRLVQIGATD 67

Query: 90  IFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSL 149
           +F+VPGD NL L DY +A  GL L+GCC+ELNAGYAADGYAR RGVGACAVTFTVG LS+
Sbjct: 68  VFAVPGDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSV 127

Query: 150 INAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDN 209
           +NAIAGAYSE+LPVICIVGGPNSNDYG+ +ILHHTIGLPDF QELRCFQ +TCYQA+I+N
Sbjct: 128 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINN 187

Query: 210 LEDAQWQIDTAISKCLEESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEA 269
           L+DA  QIDTAI+  L ESKP+YIS+ CNL  + HP+FS ++ +PL +S + SN+  LE 
Sbjct: 188 LDDAHEQIDTAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEY 247

Query: 270 AVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHF 329
           AVE AA  LN A+KPVM+GG K+R+  +++ F  +A + GYP AVMPSAKG+ PE+HP F
Sbjct: 248 AVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPFAVMPSAKGLVPEHHPRF 307

Query: 330 IGTYWGTISSTYCGETVEIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNG 389
           IGTYWG +S+T+C E VE ADA +F G I ++  +V +SL  K+ K ++V+ DRVV  NG
Sbjct: 308 IGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNG 367

Query: 390 PSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKM 449
           P+ G ILM +FL AL KRL  N+TAY+NYRRI+I +  P    P E LRVNI+FKHI++M
Sbjct: 368 PAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEM 427

Query: 450 LSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLL 509
           LS + AVI+E GDSWF+ Q+L+LP+ CGYE Q+ Y SIGWS+GATLGYAQAA +KRV+  
Sbjct: 428 LSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISC 487

Query: 510 IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMH 569
           IGDGSFQMT QDVSTM+   Q +IIFLINNGGYTIEVEIHDGPYNVIKNWDYT +++A+H
Sbjct: 488 IGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIH 547

Query: 570 NHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSS 629
           N DGNCWT KV TEEEL+ AI      +KD LCFIE+IVH+DDTSKELLE+GS+ +A +S
Sbjct: 548 NSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANS 602

Query: 630 RPP 631
           RPP
Sbjct: 608 RPP 602

BLAST of CSPI04G16020 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.5e-224
Identity = 376/586 (64.16%), Postives = 456/586 (77.82%), Query Frame = 0

Query: 49  SHNNGAITPSTVIIPPPTTT----PSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFV 108
           S  NG +      +P    T     +TLG +LARRLVQ G++D+FSVPGD NL L D+ +
Sbjct: 20  SPTNGTVATIHNSVPSSAITINYCDATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLM 79

Query: 109 AAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICI 168
           A   LNL+GCC+ELNAGYAADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LP+ICI
Sbjct: 80  AEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICI 139

Query: 169 VGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLE 228
           VGGPNSNDYG+ +ILHHTIGLPDF QELRCFQ VTCYQA+++NL+DA  QID AIS  L+
Sbjct: 140 VGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALK 199

Query: 229 ESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVM 288
           ESKP+YIS+ CNL AIPH +FS +  +P SL+ + SN+M LEAAVE   + LN A+KPVM
Sbjct: 200 ESKPVYISVSCNLAAIPHHTFS-RDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVM 259

Query: 289 IGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETV 348
           +GG KLR+  + + F+ELA+A GY +A+MPSAKG  PE+HPHFIGTYWG +S+ +C E V
Sbjct: 260 VGGPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIV 319

Query: 349 EIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGK 408
           E ADA IF G I ++  +V +SL  KK K I+V+ DR+   NGP+ G ILM DF R L K
Sbjct: 320 ESADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVANGPTFGCILMSDFFRELSK 379

Query: 409 RLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFH 468
           R+K N TAYENY RI++ E  PL+    E LRVN +F+HIQKMLSS  AVI+E GDSWF+
Sbjct: 380 RVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFN 439

Query: 469 SQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMI 528
            Q+LKLPK CGYE Q+ Y SIGWS+GATLGYAQA+PEKRV+  IGDGSFQ+T QD+STM+
Sbjct: 440 CQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTML 499

Query: 529 TLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEEL 588
              Q  IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +GNCWT KV  EEEL
Sbjct: 500 RNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEEL 559

Query: 589 VNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           V AI     E+KD LCFIEVI+H+DDTSKELLE+GS+ +A +SRPP
Sbjct: 560 VEAITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CSPI04G16020 vs. ExPASy Swiss-Prot
Match: Q9M039 (Pyruvate decarboxylase 3 OS=Arabidopsis thaliana OX=3702 GN=PDC3 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.5e-224
Identity = 378/589 (64.18%), Postives = 458/589 (77.76%), Query Frame = 0

Query: 42  LRSQSRSSHNNGAITPSTVIIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFD 101
           L S   ++  + A T +T++     ++ +TLG +L+RRLVQ G++DIF+VPGD NL L D
Sbjct: 6   LPSNGVATIQDSAPTAATIL----GSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLD 65

Query: 102 YFVAAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPV 161
             +A   LN +GCC+ELNAGYAADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LPV
Sbjct: 66  QLIANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPV 125

Query: 162 ICIVGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISK 221
           ICIVGGPNSND+G+ +ILHHTIGLPDF QELRCFQ VTCYQA++++LEDA  QID AI+ 
Sbjct: 126 ICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIAT 185

Query: 222 CLEESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIK 281
            L ESKP+YISI CNL AIPHP+F A   +P  L+ + SN+  LEAAVE   + LN A+K
Sbjct: 186 ALRESKPVYISISCNLAAIPHPTF-ASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVK 245

Query: 282 PVMIGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCG 341
           PVM+GG KLR+  +++ F+ELA+A GYPVAVMPSAKG  PENHPHFIGTYWG +S+ +C 
Sbjct: 246 PVMVGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCS 305

Query: 342 ETVEIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRA 401
           E VE ADA IF G I ++  +V +SL  KK K I+V  D VV  NGP+ G + M +F R 
Sbjct: 306 EIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRE 365

Query: 402 LGKRLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDS 461
           L KR+KPN TAYENY RI++ E  PL+  P E LR+N +F+HIQKMLS+  AVI+E GDS
Sbjct: 366 LAKRVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDS 425

Query: 462 WFHSQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVS 521
           WF+ Q+LKLPK CGYE Q+ Y SIGWS+GATLGYAQA PEKRV+  IGDGSFQ+T QDVS
Sbjct: 426 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVS 485

Query: 522 TMITLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTE 581
           TMI   Q  IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +G CWTTKV  E
Sbjct: 486 TMIRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYE 545

Query: 582 EELVNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           EELV AI     E+KDSLCFIEVIVH+DDTSKELLE+GS+ +A + RPP
Sbjct: 546 EELVEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPP 589

BLAST of CSPI04G16020 vs. ExPASy TrEMBL
Match: A0A0A0L2P1 (Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312240 PE=3 SV=1)

HSP 1 Score: 1237.6 bits (3201), Expect = 0.0e+00
Identity = 631/698 (90.40%), Postives = 631/698 (90.40%), Query Frame = 0

Query: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60
           MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV
Sbjct: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60

Query: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNA 120
           IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVA KGLNLVGCCSELNA
Sbjct: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAEKGLNLVGCCSELNA 120

Query: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180
           GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH
Sbjct: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180

Query: 181 HTIGLPDFGQELRCFQNVTCYQ-------------------------------------- 240
           HTIGLPDFGQELRCFQNVTCYQ                                      
Sbjct: 181 HTIGLPDFGQELRCFQNVTCYQVATKSKYFLKYWNMIFRVTQPTTQRQDIGALYYDGSHS 240

Query: 241 --------------------------AIIDNLEDAQWQIDTAISKCLEESKPIYISICCN 300
                                     AIIDNLEDAQWQIDTAISKCLEESKPIYISICCN
Sbjct: 241 LSSLSPDPARFKHPSVIVWVKKQKQKAIIDNLEDAQWQIDTAISKCLEESKPIYISICCN 300

Query: 301 LVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQ 360
           LVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQ
Sbjct: 301 LVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQ 360

Query: 361 EPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAI 420
           EPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAI
Sbjct: 361 EPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAI 420

Query: 421 LDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENY 480
           LDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENY
Sbjct: 421 LDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENY 480

Query: 481 RRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGY 540
           RRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGY
Sbjct: 481 RRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGY 540

Query: 541 EVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLIN 600
           EVQLLYASIGWSLGATLGYAQAAPEKRVVL IGDGSFQMTPQDVSTMITLKQNNIIFLIN
Sbjct: 541 EVQLLYASIGWSLGATLGYAQAAPEKRVVLFIGDGSFQMTPQDVSTMITLKQNNIIFLIN 600

Query: 601 NGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERK 635
           NGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERK
Sbjct: 601 NGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERK 660

BLAST of CSPI04G16020 vs. ExPASy TrEMBL
Match: A0A5D3E0N5 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00060 PE=3 SV=1)

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 581/631 (92.08%), Postives = 599/631 (94.93%), Query Frame = 0

Query: 4   KLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSR-SSHNNGAITPSTVII 63
           K+ENGF TETNP VTTT+ ASHSPTV PLANGIAAADKLRSQSR ++HNNGA+T     I
Sbjct: 16  KVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNHNNGAVT-----I 75

Query: 64  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 123
           P PTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLV+FDYFVA KGLNLVGCC+ELNAGY
Sbjct: 76  PTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGY 135

Query: 124 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 183
           AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT
Sbjct: 136 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 195

Query: 184 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 243
           IGLPDFGQELRCFQNVTCYQAIIDNLEDAQW+IDTAISKCLEE KPIYISICCNLVAIPH
Sbjct: 196 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPH 255

Query: 244 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 303
           PSFSAQ LIPLSLS K+SNQMALEAAVEKAAQLLNTAIKPVMIGG+KLR T SQEPFIEL
Sbjct: 256 PSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIEL 315

Query: 304 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 363
           ANACGYPVAV PSAKGMFPENHPHFIG YWGT+SS YCGETVEIADASIFVGA LDEL+T
Sbjct: 316 ANACGYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELET 375

Query: 364 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 423
           VAF+LTYKKNK IMVEADRVVFPNGPS GPILMKDFL AL KRLKPNSTAYENYRRIYIA
Sbjct: 376 VAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIA 435

Query: 424 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 483
           ESGPLESGPGEALRVNI+FKHIQKMLSSNM VISEAGDSWFH QRLKLPKSCGYEVQLLY
Sbjct: 436 ESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLY 495

Query: 484 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 543
           ASIGWSLGATLGYAQAAPE+RVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI
Sbjct: 496 ASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 555

Query: 544 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 603
           EVEIHDGPYNVIKNWDYTAIV AMHNHDG CWTTKVHTEEEL+NAIEIVL+ERKD LCFI
Sbjct: 556 EVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFI 615

Query: 604 EVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           EVIVHRDDTS+ELLEFGSKFAAVSSRPPR L
Sbjct: 616 EVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of CSPI04G16020 vs. ExPASy TrEMBL
Match: A0A5A7T827 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00370 PE=3 SV=1)

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 580/631 (91.92%), Postives = 599/631 (94.93%), Query Frame = 0

Query: 4   KLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSR-SSHNNGAITPSTVII 63
           K+ENGF TETNP VTTT+ ASHSPTV PLANGIAAADKLRSQSR ++HNNGA+T     I
Sbjct: 16  KVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNHNNGAVT-----I 75

Query: 64  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 123
           P PTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLV+FDYFVA KGLNLVGCC+ELNAGY
Sbjct: 76  PTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGY 135

Query: 124 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 183
           AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT
Sbjct: 136 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 195

Query: 184 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 243
           IGLPDFGQELRCFQNVTCYQAIIDNLEDAQW+IDTAISKCLEE KPIYISICCNLVAIPH
Sbjct: 196 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPH 255

Query: 244 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 303
           PSFSAQ LIPLSLS K+SNQMALEAAVEKAAQLLNTAIKPVMIGG+KLR T SQEPFIEL
Sbjct: 256 PSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIEL 315

Query: 304 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 363
           ANACGYPVAV PSAKGMFPE+HPHFIG YWGT+SS YCGETVEIADASIFVGA LDEL+T
Sbjct: 316 ANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELET 375

Query: 364 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 423
           VAF+LTYKKNK IMVEADRVVFPNGPS GPILMKDFL AL KRLKPNSTAYENYRRIYIA
Sbjct: 376 VAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIA 435

Query: 424 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 483
           ESGPLESGPGEALRVNI+FKHIQKMLSSNM VISEAGDSWFH QRLKLPKSCGYEVQLLY
Sbjct: 436 ESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLY 495

Query: 484 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 543
           ASIGWSLGATLGYAQAAPE+RVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI
Sbjct: 496 ASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 555

Query: 544 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 603
           EVEIHDGPYNVIKNWDYTAIV AMHNHDG CWTTKVHTEEEL+NAIEIVL+ERKD LCFI
Sbjct: 556 EVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFI 615

Query: 604 EVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           EVIVHRDDTS+ELLEFGSKFAAVSSRPPR L
Sbjct: 616 EVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of CSPI04G16020 vs. ExPASy TrEMBL
Match: A0A1S3CKZ0 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502148 PE=3 SV=1)

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 580/631 (91.92%), Postives = 599/631 (94.93%), Query Frame = 0

Query: 4   KLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSR-SSHNNGAITPSTVII 63
           K+ENGF TETNP VTTT+ ASHSPTV PLANGIAAADKLRSQSR ++HNNGA+T     I
Sbjct: 16  KVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNHNNGAVT-----I 75

Query: 64  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 123
           P PTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLV+FDYFVA KGLNLVGCC+ELNAGY
Sbjct: 76  PTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGY 135

Query: 124 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 183
           AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT
Sbjct: 136 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 195

Query: 184 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 243
           IGLPDFGQELRCFQNVTCYQAIIDNLEDAQW+IDTAISKCLEE KPIYISICCNLVAIPH
Sbjct: 196 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPH 255

Query: 244 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 303
           PSFSAQ LIPLSLS K+SNQMALEAAVEKAAQLLNTAIKPVMIGG+KLR T SQEPFIEL
Sbjct: 256 PSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIEL 315

Query: 304 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 363
           ANACGYPVAV PSAKGMFPE+HPHFIG YWGT+SS YCGETVEIADASIFVGA LDEL+T
Sbjct: 316 ANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELET 375

Query: 364 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 423
           VAF+LTYKKNK IMVEADRVVFPNGPS GPILMKDFL AL KRLKPNSTAYENYRRIYIA
Sbjct: 376 VAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIA 435

Query: 424 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 483
           ESGPLESGPGEALRVNI+FKHIQKMLSSNM VISEAGDSWFH QRLKLPKSCGYEVQLLY
Sbjct: 436 ESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLY 495

Query: 484 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 543
           ASIGWSLGATLGYAQAAPE+RVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI
Sbjct: 496 ASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 555

Query: 544 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 603
           EVEIHDGPYNVIKNWDYTAIV AMHNHDG CWTTKVHTEEEL+NAIEIVL+ERKD LCFI
Sbjct: 556 EVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFI 615

Query: 604 EVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           EVIVHRDDTS+ELLEFGSKFAAVSSRPPR L
Sbjct: 616 EVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of CSPI04G16020 vs. ExPASy TrEMBL
Match: A0A5D3E1K9 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00080 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.6e-293
Identity = 500/627 (79.74%), Postives = 548/627 (87.40%), Query Frame = 0

Query: 7   NGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTVIIPPPT 66
           N F T+T P       A+++P +  + NG  A+DKL     SS+NNG+     VI+PPP 
Sbjct: 22  NHFQTQTRPM------AAYNPRIEHVTNGSLASDKL----ISSNNNGS---PLVIVPPPV 81

Query: 67  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGYAADG 126
           T PSTLGHYLARRLV+IG+SDIFSVPGDSNLVLFDYFVA KGLNLVGCC+ELNAGYAADG
Sbjct: 82  TVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADG 141

Query: 127 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 186
           YAR RGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP
Sbjct: 142 YARNRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 201

Query: 187 DFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPHPSFS 246
           DF QELRCFQNVTCYQA+IDNLEDAQWQID AI KCLEESKP+YISICCNLVAIPHPSFS
Sbjct: 202 DFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFS 261

Query: 247 AQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIELANAC 306
           A+ LIPLSLS K+SNQM LE AVEKAA LLNTAIKPVMIGG+KLR   ++  F+ELAN C
Sbjct: 262 AKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLELANTC 321

Query: 307 GYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDTVAFS 366
           GY VAV PSAKGMFPENHPHFIGTYWGT+S+ +CGETVEIADASIFVGA LDEL+TV +S
Sbjct: 322 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETVGYS 381

Query: 367 LTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIAESGP 426
           LTYKKNK IMV+ D VVFPNG S G + +KDFLRALGKRLKPN  AYENYRRIYIAESGP
Sbjct: 382 LTYKKNKAIMVKPDGVVFPNGASYGGVQIKDFLRALGKRLKPNLRAYENYRRIYIAESGP 441

Query: 427 LESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLYASIG 486
            ES  GE LRVN++FKHIQKMLSSNM VI+E GDSWFHSQ+LKLPKSCGYE+QLLYASIG
Sbjct: 442 PESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEIQLLYASIG 501

Query: 487 WSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 546
           WSLGATLGYAQ+AP+KR+VL IGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 502 WSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLINNGGYTIEVEI 561

Query: 547 HDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFIEVIV 606
           HDGPYN+I NWDYTA V A++NH GNCWTTKVHTEEELV AIEI +++R D LCFIEVIV
Sbjct: 562 HDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDRNDYLCFIEVIV 621

Query: 607 HRDDTSKELLEFGSKFAAVSSRPPRVL 634
           HRDDTSKELLEFGS+ AA+ S PP++L
Sbjct: 622 HRDDTSKELLEFGSRIAAMGSHPPKLL 635

BLAST of CSPI04G16020 vs. NCBI nr
Match: XP_004148632.1 (pyruvate decarboxylase 1 [Cucumis sativus] >KAE8649566.1 hypothetical protein Csa_018180 [Cucumis sativus])

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 631/634 (99.53%), Postives = 631/634 (99.53%), Query Frame = 0

Query: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60
           MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV
Sbjct: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60

Query: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNA 120
           IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVA KGLNLVGCCSELNA
Sbjct: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAEKGLNLVGCCSELNA 120

Query: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180
           GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH
Sbjct: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180

Query: 181 HTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAI 240
           HTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAI
Sbjct: 181 HTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAI 240

Query: 241 PHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFI 300
           PHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFI
Sbjct: 241 PHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFI 300

Query: 301 ELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDEL 360
           ELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDEL
Sbjct: 301 ELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDEL 360

Query: 361 DTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIY 420
           DTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIY
Sbjct: 361 DTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIY 420

Query: 421 IAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQL 480
           IAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQL
Sbjct: 421 IAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQL 480

Query: 481 LYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540
           LYASIGWSLGATLGYAQAAPEKRVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY
Sbjct: 481 LYASIGWSLGATLGYAQAAPEKRVVLFIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540

Query: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLC 600
           TIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLC
Sbjct: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLC 600

Query: 601 FIEVIVHRDDTSKELLEFGSKFAAVSSRPPRVLL 635
           FIEVIVHRDDTSKELLEFGSKFA VSSRPPRVLL
Sbjct: 601 FIEVIVHRDDTSKELLEFGSKFANVSSRPPRVLL 634

BLAST of CSPI04G16020 vs. NCBI nr
Match: TYK29657.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 581/631 (92.08%), Postives = 599/631 (94.93%), Query Frame = 0

Query: 4   KLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSR-SSHNNGAITPSTVII 63
           K+ENGF TETNP VTTT+ ASHSPTV PLANGIAAADKLRSQSR ++HNNGA+T     I
Sbjct: 16  KVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNHNNGAVT-----I 75

Query: 64  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 123
           P PTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLV+FDYFVA KGLNLVGCC+ELNAGY
Sbjct: 76  PTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGY 135

Query: 124 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 183
           AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT
Sbjct: 136 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 195

Query: 184 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 243
           IGLPDFGQELRCFQNVTCYQAIIDNLEDAQW+IDTAISKCLEE KPIYISICCNLVAIPH
Sbjct: 196 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPH 255

Query: 244 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 303
           PSFSAQ LIPLSLS K+SNQMALEAAVEKAAQLLNTAIKPVMIGG+KLR T SQEPFIEL
Sbjct: 256 PSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIEL 315

Query: 304 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 363
           ANACGYPVAV PSAKGMFPENHPHFIG YWGT+SS YCGETVEIADASIFVGA LDEL+T
Sbjct: 316 ANACGYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELET 375

Query: 364 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 423
           VAF+LTYKKNK IMVEADRVVFPNGPS GPILMKDFL AL KRLKPNSTAYENYRRIYIA
Sbjct: 376 VAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIA 435

Query: 424 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 483
           ESGPLESGPGEALRVNI+FKHIQKMLSSNM VISEAGDSWFH QRLKLPKSCGYEVQLLY
Sbjct: 436 ESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLY 495

Query: 484 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 543
           ASIGWSLGATLGYAQAAPE+RVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI
Sbjct: 496 ASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 555

Query: 544 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 603
           EVEIHDGPYNVIKNWDYTAIV AMHNHDG CWTTKVHTEEEL+NAIEIVL+ERKD LCFI
Sbjct: 556 EVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFI 615

Query: 604 EVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           EVIVHRDDTS+ELLEFGSKFAAVSSRPPR L
Sbjct: 616 EVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of CSPI04G16020 vs. NCBI nr
Match: XP_008464213.1 (PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037741.1 pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 580/631 (91.92%), Postives = 599/631 (94.93%), Query Frame = 0

Query: 4   KLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSR-SSHNNGAITPSTVII 63
           K+ENGF TETNP VTTT+ ASHSPTV PLANGIAAADKLRSQSR ++HNNGA+T     I
Sbjct: 16  KVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNHNNGAVT-----I 75

Query: 64  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 123
           P PTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLV+FDYFVA KGLNLVGCC+ELNAGY
Sbjct: 76  PTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGY 135

Query: 124 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 183
           AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT
Sbjct: 136 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 195

Query: 184 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 243
           IGLPDFGQELRCFQNVTCYQAIIDNLEDAQW+IDTAISKCLEE KPIYISICCNLVAIPH
Sbjct: 196 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPH 255

Query: 244 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 303
           PSFSAQ LIPLSLS K+SNQMALEAAVEKAAQLLNTAIKPVMIGG+KLR T SQEPFIEL
Sbjct: 256 PSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIEL 315

Query: 304 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 363
           ANACGYPVAV PSAKGMFPE+HPHFIG YWGT+SS YCGETVEIADASIFVGA LDEL+T
Sbjct: 316 ANACGYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELET 375

Query: 364 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 423
           VAF+LTYKKNK IMVEADRVVFPNGPS GPILMKDFL AL KRLKPNSTAYENYRRIYIA
Sbjct: 376 VAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIA 435

Query: 424 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 483
           ESGPLESGPGEALRVNI+FKHIQKMLSSNM VISEAGDSWFH QRLKLPKSCGYEVQLLY
Sbjct: 436 ESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLY 495

Query: 484 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 543
           ASIGWSLGATLGYAQAAPE+RVVL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI
Sbjct: 496 ASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 555

Query: 544 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 603
           EVEIHDGPYNVIKNWDYTAIV AMHNHDG CWTTKVHTEEEL+NAIEIVL+ERKD LCFI
Sbjct: 556 EVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFI 615

Query: 604 EVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           EVIVHRDDTS+ELLEFGSKFAAVSSRPPR L
Sbjct: 616 EVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of CSPI04G16020 vs. NCBI nr
Match: XP_038881352.1 (pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 548/633 (86.57%), Postives = 581/633 (91.79%), Query Frame = 0

Query: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60
           M+ KLENGFHTET PK T         T+ P+ANG+AAADKLRSQSRS  NNGA  PS V
Sbjct: 1   MSMKLENGFHTETMPKAT---------TIKPIANGVAAADKLRSQSRSIDNNGAAAPSPV 60

Query: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNA 120
           IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVA +GLNLVGCC+ELNA
Sbjct: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAERGLNLVGCCNELNA 120

Query: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180
           GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH
Sbjct: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180

Query: 181 HTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAI 240
           HTIGLPDF QELRCFQNVTCYQAIIDNLEDAQWQID AISKCLEE KP+YISICCNLVAI
Sbjct: 181 HTIGLPDFSQELRCFQNVTCYQAIIDNLEDAQWQIDRAISKCLEERKPVYISICCNLVAI 240

Query: 241 PHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFI 300
           PHPSFSAQQLIPLSLS K+SNQMALE AVEKAAQLLN+AIKPVMIGG+KLR   +QE F+
Sbjct: 241 PHPSFSAQQLIPLSLSPKKSNQMALEVAVEKAAQLLNSAIKPVMIGGKKLRPAKAQEAFM 300

Query: 301 ELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDEL 360
           ELA+AC Y VAV PSAKGMFPE HPHFIGTYWGT+S+ YCGETVEIADASIF GA LDEL
Sbjct: 301 ELADACRYAVAVTPSAKGMFPEYHPHFIGTYWGTVSTAYCGETVEIADASIFAGATLDEL 360

Query: 361 DTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIY 420
           +TVAFSLTYKKNK I+VEADRVVFPNGPS GPILMKDFL A+ KRL PN TAYENYRRIY
Sbjct: 361 ETVAFSLTYKKNKAIIVEADRVVFPNGPSFGPILMKDFLSAIAKRLNPNPTAYENYRRIY 420

Query: 421 IAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQL 480
           IAESGPL+S PGEALRVN++FKHIQKMLSSN+ VISE GDSWF  Q+LKLPKSCGYE+QL
Sbjct: 421 IAESGPLQSNPGEALRVNVLFKHIQKMLSSNIVVISETGDSWFQCQKLKLPKSCGYEIQL 480

Query: 481 LYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540
           LYASIGWSLGATLGYAQAAPEKR+VL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY
Sbjct: 481 LYASIGWSLGATLGYAQAAPEKRMVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540

Query: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLC 600
           TIEVEIHDGPYNVIKNWDYTAIVNAMHN DGNCWTTK+HTEEELVNAIE  + ++KD LC
Sbjct: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNRDGNCWTTKIHTEEELVNAIEKAMGDQKDCLC 600

Query: 601 FIEVIVHRDDTSKELLEFGSKFAAVSSRPPRVL 634
           FIEVI+HRDDTSKELLEFGSKFAA+SSRPPR++
Sbjct: 601 FIEVIIHRDDTSKELLEFGSKFAAISSRPPRLV 624

BLAST of CSPI04G16020 vs. NCBI nr
Match: XP_038881353.1 (pyruvate decarboxylase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.6e-295
Identity = 504/577 (87.35%), Postives = 529/577 (91.68%), Query Frame = 0

Query: 1   MATKLENGFHTETNPKVTTTMEASHSPTVNPLANGIAAADKLRSQSRSSHNNGAITPSTV 60
           M+ KLENGFHTET PK T         T+ P+ANG+AAADKLRSQSRS  NNGA  PS V
Sbjct: 1   MSMKLENGFHTETMPKAT---------TIKPIANGVAAADKLRSQSRSIDNNGAAAPSPV 60

Query: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNA 120
           IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVA +GLNLVGCC+ELNA
Sbjct: 61  IIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAERGLNLVGCCNELNA 120

Query: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180
           GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH
Sbjct: 121 GYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILH 180

Query: 181 HTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAI 240
           HTIGLPDF QELRCFQNVTCYQAIIDNLEDAQWQID AISKCLEE KP+YISICCNLVAI
Sbjct: 181 HTIGLPDFSQELRCFQNVTCYQAIIDNLEDAQWQIDRAISKCLEERKPVYISICCNLVAI 240

Query: 241 PHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFI 300
           PHPSFSAQQLIPLSLS K+SNQMALE AVEKAAQLLN+AIKPVMIGG+KLR   +QE F+
Sbjct: 241 PHPSFSAQQLIPLSLSPKKSNQMALEVAVEKAAQLLNSAIKPVMIGGKKLRPAKAQEAFM 300

Query: 301 ELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDEL 360
           ELA+AC Y VAV PSAKGMFPE HPHFIGTYWGT+S+ YCGETVEIADASIF GA LDEL
Sbjct: 301 ELADACRYAVAVTPSAKGMFPEYHPHFIGTYWGTVSTAYCGETVEIADASIFAGATLDEL 360

Query: 361 DTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIY 420
           +TVAFSLTYKKNK I+VEADRVVFPNGPS GPILMKDFL A+ KRL PN TAYENYRRIY
Sbjct: 361 ETVAFSLTYKKNKAIIVEADRVVFPNGPSFGPILMKDFLSAIAKRLNPNPTAYENYRRIY 420

Query: 421 IAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQL 480
           IAESGPL+S PGEALRVN++FKHIQKMLSSN+ VISE GDSWF  Q+LKLPKSCGYE+QL
Sbjct: 421 IAESGPLQSNPGEALRVNVLFKHIQKMLSSNIVVISETGDSWFQCQKLKLPKSCGYEIQL 480

Query: 481 LYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540
           LYASIGWSLGATLGYAQAAPEKR+VL IGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY
Sbjct: 481 LYASIGWSLGATLGYAQAAPEKRMVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGY 540

Query: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTK 578
           TIEVEIHDGPYNVIKNWDYTAIVNAMHN DGNCWTTK
Sbjct: 541 TIEVEIHDGPYNVIKNWDYTAIVNAMHNRDGNCWTTK 568

BLAST of CSPI04G16020 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 798.1 bits (2060), Expect = 5.1e-231
Identity = 381/568 (67.08%), Postives = 453/568 (79.75%), Query Frame = 0

Query: 63  PPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGY 122
           P  TT+ STLG +L+RRLVQ G++D+FSVPGD NL L D+ +A   LN +GCC+ELNAGY
Sbjct: 34  PITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGY 93

Query: 123 AADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 182
           AADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LPVICIVGGPNSND+G+ +ILHHT
Sbjct: 94  AADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHT 153

Query: 183 IGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESKPIYISICCNLVAIPH 242
           IGLPDF QELRCFQ VTCYQA+++NLEDA  QID AI+  L+ESKP+YISI CNL A PH
Sbjct: 154 IGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPH 213

Query: 243 PSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGGRKLRLTNSQEPFIEL 302
           P+F A+  +P  L+ + SN M LEAAVE   + LN A+KPVM+GG KLR+  + E F+EL
Sbjct: 214 PTF-ARDPVPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLEL 273

Query: 303 ANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDT 362
           A+A GYP+AVMPS KG+ PENHPHFIGTYWG +S+ +C E VE ADA IF G I ++  +
Sbjct: 274 ADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSS 333

Query: 363 VAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLKPNSTAYENYRRIYIA 422
           V +SL  KK K I+V  DRVV  NGP+ G +LM DF R L KR+K N TAYENY RI++ 
Sbjct: 334 VGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVP 393

Query: 423 ESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQRLKLPKSCGYEVQLLY 482
           E  PL+  PGE LRVN +F+HIQKMLSS  AVI+E GDSWF+ Q+LKLPK CGYE Q+ Y
Sbjct: 394 EGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQY 453

Query: 483 ASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTI 542
            SIGWS+GATLGYAQA PEKRV+  IGDGSFQ+T QD+STMI   Q  IIFLINNGGYTI
Sbjct: 454 GSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTI 513

Query: 543 EVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNAIEIVLKERKDSLCFI 602
           EVEIHDGPYNVIKNW+YT +V+A+HN +G CWTTKV  EEELV AI+    E+KDSLCFI
Sbjct: 514 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFI 573

Query: 603 EVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           EVIVH+DDTSKELLE+GS+ +A + RPP
Sbjct: 574 EVIVHKDDTSKELLEWGSRVSAANGRPP 600

BLAST of CSPI04G16020 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 780.4 bits (2014), Expect = 1.1e-225
Identity = 376/586 (64.16%), Postives = 456/586 (77.82%), Query Frame = 0

Query: 49  SHNNGAITPSTVIIPPPTTT----PSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFV 108
           S  NG +      +P    T     +TLG +LARRLVQ G++D+FSVPGD NL L D+ +
Sbjct: 20  SPTNGTVATIHNSVPSSAITINYCDATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLM 79

Query: 109 AAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICI 168
           A   LNL+GCC+ELNAGYAADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LP+ICI
Sbjct: 80  AEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICI 139

Query: 169 VGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLE 228
           VGGPNSNDYG+ +ILHHTIGLPDF QELRCFQ VTCYQA+++NL+DA  QID AIS  L+
Sbjct: 140 VGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALK 199

Query: 229 ESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVM 288
           ESKP+YIS+ CNL AIPH +FS +  +P SL+ + SN+M LEAAVE   + LN A+KPVM
Sbjct: 200 ESKPVYISVSCNLAAIPHHTFS-RDPVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVM 259

Query: 289 IGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETV 348
           +GG KLR+  + + F+ELA+A GY +A+MPSAKG  PE+HPHFIGTYWG +S+ +C E V
Sbjct: 260 VGGPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIV 319

Query: 349 EIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGK 408
           E ADA IF G I ++  +V +SL  KK K I+V+ DR+   NGP+ G ILM DF R L K
Sbjct: 320 ESADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVANGPTFGCILMSDFFRELSK 379

Query: 409 RLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFH 468
           R+K N TAYENY RI++ E  PL+    E LRVN +F+HIQKMLSS  AVI+E GDSWF+
Sbjct: 380 RVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFN 439

Query: 469 SQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMI 528
            Q+LKLPK CGYE Q+ Y SIGWS+GATLGYAQA+PEKRV+  IGDGSFQ+T QD+STM+
Sbjct: 440 CQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTML 499

Query: 529 TLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEEL 588
              Q  IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +GNCWT KV  EEEL
Sbjct: 500 RNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEEL 559

Query: 589 VNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           V AI     E+KD LCFIEVI+H+DDTSKELLE+GS+ +A +SRPP
Sbjct: 560 VEAITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CSPI04G16020 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 780.4 bits (2014), Expect = 1.1e-225
Identity = 378/589 (64.18%), Postives = 458/589 (77.76%), Query Frame = 0

Query: 42  LRSQSRSSHNNGAITPSTVIIPPPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFD 101
           L S   ++  + A T +T++     ++ +TLG +L+RRLVQ G++DIF+VPGD NL L D
Sbjct: 6   LPSNGVATIQDSAPTAATIL----GSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLD 65

Query: 102 YFVAAKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPV 161
             +A   LN +GCC+ELNAGYAADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LPV
Sbjct: 66  QLIANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPV 125

Query: 162 ICIVGGPNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISK 221
           ICIVGGPNSND+G+ +ILHHTIGLPDF QELRCFQ VTCYQA++++LEDA  QID AI+ 
Sbjct: 126 ICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIAT 185

Query: 222 CLEESKPIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIK 281
            L ESKP+YISI CNL AIPHP+F A   +P  L+ + SN+  LEAAVE   + LN A+K
Sbjct: 186 ALRESKPVYISISCNLAAIPHPTF-ASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVK 245

Query: 282 PVMIGGRKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCG 341
           PVM+GG KLR+  +++ F+ELA+A GYPVAVMPSAKG  PENHPHFIGTYWG +S+ +C 
Sbjct: 246 PVMVGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCS 305

Query: 342 ETVEIADASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRA 401
           E VE ADA IF G I ++  +V +SL  KK K I+V  D VV  NGP+ G + M +F R 
Sbjct: 306 EIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRE 365

Query: 402 LGKRLKPNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDS 461
           L KR+KPN TAYENY RI++ E  PL+  P E LR+N +F+HIQKMLS+  AVI+E GDS
Sbjct: 366 LAKRVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDS 425

Query: 462 WFHSQRLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVS 521
           WF+ Q+LKLPK CGYE Q+ Y SIGWS+GATLGYAQA PEKRV+  IGDGSFQ+T QDVS
Sbjct: 426 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVS 485

Query: 522 TMITLKQNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTE 581
           TMI   Q  IIFLINNGGYTIEVEIHDGPYNVIKNW+YT +V+A+HN +G CWTTKV  E
Sbjct: 486 TMIRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYE 545

Query: 582 EELVNAIEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           EELV AI     E+KDSLCFIEVIVH+DDTSKELLE+GS+ +A + RPP
Sbjct: 546 EELVEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPP 589

BLAST of CSPI04G16020 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 777.7 bits (2007), Expect = 7.1e-225
Identity = 375/583 (64.32%), Postives = 454/583 (77.87%), Query Frame = 0

Query: 52  NGAITPSTVIIPPPTTTPS----TLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAAK 111
           NG ++      P  +TT S    TLG YLARRLV+IG++D+FSVPGD NL L D+ +A  
Sbjct: 23  NGGVSTVQNTSPLHSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEP 82

Query: 112 GLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGG 171
            L L+GCC+ELNAGYAADGYAR RGVGAC VTFTVG LS++NAIAGAYSE+LP+ICIVGG
Sbjct: 83  NLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGG 142

Query: 172 PNSNDYGSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWQIDTAISKCLEESK 231
           PNSNDYG+ +ILHHTIGLPDF QELRCFQ VTC+QA+I+NLE+A   IDTAIS  L+ESK
Sbjct: 143 PNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESK 202

Query: 232 PIYISICCNLVAIPHPSFSAQQLIPLSLSSKESNQMALEAAVEKAAQLLNTAIKPVMIGG 291
           P+YISI CNL AIP P+FS +  +P  L  K SNQ+ L+AAVE AA+ LN A+KPV++GG
Sbjct: 203 PVYISISCNLPAIPLPTFS-RHPVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGG 262

Query: 292 RKLRLTNSQEPFIELANACGYPVAVMPSAKGMFPENHPHFIGTYWGTISSTYCGETVEIA 351
            K+R+  + + F+ELA+A GY +AVMPSAKG  PE+H HFIGTYWG +S+ +C E VE A
Sbjct: 263 PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESA 322

Query: 352 DASIFVGAILDELDTVAFSLTYKKNKGIMVEADRVVFPNGPSLGPILMKDFLRALGKRLK 411
           DA +F G I ++  +V +SL  KK K I+V+ DRV   NGP+ G +LMKDFL  L KR+K
Sbjct: 323 DAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIK 382

Query: 412 PNSTAYENYRRIYIAESGPLESGPGEALRVNIVFKHIQKMLSSNMAVISEAGDSWFHSQR 471
            N+T+YENY RIY+ E  PL   P E+LRVN++F+HIQ MLSS  AV++E GDSWF+ Q+
Sbjct: 383 HNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQK 442

Query: 472 LKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLK 531
           LKLP+ CGYE Q+ Y SIGWS+GATLGYAQA P +RV+  IGDGSFQ+T QDVSTMI   
Sbjct: 443 LKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCG 502

Query: 532 QNNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTAIVNAMHNHDGNCWTTKVHTEEELVNA 591
           Q  IIFLINNGGYTIEVEIHDGPYNVIKNW+YTA V A+HN +G CWT KV  EEELV A
Sbjct: 503 QKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKA 562

Query: 592 IEIVLKERKDSLCFIEVIVHRDDTSKELLEFGSKFAAVSSRPP 631
           I     E K+S CFIEVIVH+DDTSKELLE+GS+ +A +SRPP
Sbjct: 563 INTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of CSPI04G16020 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 78.6 bits (192), Expect = 2.1e-14
Identity = 114/534 (21.35%), Postives = 220/534 (41.20%), Query Frame = 0

Query: 42  LRSQSRSSHNNGAITPSTVIIPPPTTTPSTLGHYLAR---------------RLVQIGIS 101
           ++S S SS +    T + V   P  T P+    +++R                L + G+ 
Sbjct: 54  IKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGADILVEALERQGVE 113

Query: 102 DIFSVPGDSNLVLFDYFVAAKGLNLVGCCSELNAGYAADGYARRRG-VGACAVTFTVGSL 161
            +F+ PG +++ +      +  +  V    E    +AA+GYAR  G  G C  T   G+ 
Sbjct: 114 TVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGAT 173

Query: 162 SLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFGQE--LRCFQNVTCYQA 221
           +L++ +A A  + +P++ I G          ++    IG   F +   +   +++T +  
Sbjct: 174 NLVSGLADALLDSVPLVAITG----------QVPRRMIGTDAFQETPIVEVTRSITKHNY 233

Query: 222 IIDNLEDAQWQIDTAISKCLE-ESKPIYISICCNL---VAIPHPSFSAQQLIPLSLSSKE 281
           ++ ++ED    I+ A          P+ + +  ++   +AIP    + +Q + L      
Sbjct: 234 LVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIP----NWEQAMRLPGYMSR 293

Query: 282 SNQMALEAAVEKAAQLLNTAIKPVM-IGGRKLRLTNSQEPFIELANACGYPVAVMPSAKG 341
             +   ++ +E+  +L++ + KPV+ +GG  L  ++    F+EL    G PVA      G
Sbjct: 294 MPKPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVELT---GIPVASTLMGLG 353

Query: 342 MFP---ENHPHFIGTYWGTISSTYCGETVEIADASIFVGAILDELDTVAFSLTYKKNKGI 401
            +P   E   H +G + GT+ + Y    VE +D  +  G   D+  T        + K +
Sbjct: 354 SYPCDDELSLHMLGMH-GTVYANY---AVEHSDLLLAFGVRFDDRVTGKLEAFASRAKIV 413

Query: 402 MVEADRVVF-----PNGPSLGPIL-----MKDFLRALGKRLKPNSTAYENYRRIYIAESG 461
            ++ D         P+    G +      M   L    + LK +   + N   +   +  
Sbjct: 414 HIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELNVQ-KQKF 473

Query: 462 PLESGP-GEALRVNIVFKHIQKMLSSNMAVISEAGD-SWFHSQRLKLPKSCGYEVQLLYA 521
           PL     GEA+      K + ++      + +  G    + +Q     K   +       
Sbjct: 474 PLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKKPRQWLSSGGLG 533

Query: 522 SIGWSLGATLGYAQAAPEKRVVLLIGDGSFQMTPQDVSTMITLKQNNIIFLINN 538
           ++G+ L A +G + A P+  VV + GDGSF M  Q+++T+        + L+NN
Sbjct: 534 AMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVLLLNN 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0407.1e-23067.08Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1[more]
A2Y5L93.1e-22562.85Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
Q0DHF69.0e-22562.85Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
O826471.5e-22464.16Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1[more]
Q9M0391.5e-22464.18Pyruvate decarboxylase 3 OS=Arabidopsis thaliana OX=3702 GN=PDC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2P10.0e+0090.40Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312240 PE=3 SV=1[more]
A0A5D3E0N50.0e+0092.08Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
A0A5A7T8270.0e+0091.92Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3CKZ00.0e+0091.92Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502148 PE=3 SV=1[more]
A0A5D3E1K91.6e-29379.74Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
Match NameE-valueIdentityDescription
XP_004148632.10.0e+0099.53pyruvate decarboxylase 1 [Cucumis sativus] >KAE8649566.1 hypothetical protein Cs... [more]
TYK29657.10.0e+0092.08pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_008464213.10.0e+0091.92PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037741.1 pyruvate d... [more]
XP_038881352.10.0e+0086.57pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida][more]
XP_038881353.13.6e-29587.35pyruvate decarboxylase 1-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G01320.15.1e-23167.08Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT4G33070.11.1e-22564.16Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01330.11.1e-22564.18pyruvate decarboxylase-3 [more]
AT5G54960.17.1e-22564.32pyruvate decarboxylase-2 [more]
AT3G48560.12.1e-1421.35chlorsulfuron/imidazolinone resistant 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 66..628
e-value: 1.8E-190
score: 630.8
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 45..631
NoneNo IPR availableGENE3D3.40.50.970coord: 67..245
e-value: 4.9E-57
score: 194.4
NoneNo IPR availableGENE3D3.40.50.970coord: 429..619
e-value: 2.8E-53
score: 182.1
NoneNo IPR availableGENE3D3.40.50.1220coord: 258..421
e-value: 9.1E-38
score: 131.4
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 71..615
e-value: 3.7E-165
score: 547.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..63
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 45..631
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 75..235
e-value: 1.13775E-86
score: 265.512
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 434..616
e-value: 8.99461E-81
score: 250.914
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 269..381
e-value: 3.7E-20
score: 72.0
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 71..238
e-value: 1.6E-31
score: 109.2
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 479..604
e-value: 3.4E-16
score: 59.4
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 493..512
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 436..626
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 71..244
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 264..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G16020.1CSPI04G16020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity