CSPI04G15140 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G15140
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein MIZU-KUSSEI 1
Location	Chr4: 12603655 .. 12604401 (+)
RNA-Seq Expression	CSPI04G15140
Synteny	CSPI04G15140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCCTGTTTCTTTCCCCCCACCGCCGCCACCGCCGCCGCCTCCTCCGCTGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACCTCGAAACCACCACCGCCACCCTCAATGAAGATCCAACCCATGGCCAAGATTCTCCCGCCACCACTCCCGACAGCGTCACACCCAAATTCGCTGTCTCTGCCTCCATTGTAGCCCCACCCCGTCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCATCGGAGAGGACACGTTTGGTTCTGTGTCCAACATGACCGTCTCCGTAACAAACCGTTTCTCCTACTCGAGTTTCCGATTCTGACTCACCAACTCGTGAATGAAATGCGATTCGGACTGGTTCGAATCGCTCTCGAGTGTAACCGGGTGGAACTGGGGTTCTGCCCACTTCGTTCGATTCCTATTTGGGCAATGTCATGTAATGGGCGAAAACTGGGGTTCGCGGCTAAGAAAAAAGCCGGTGAACCGGTCCGGTCCATGTTGAAGACAATGCAATCGACAACGGTGGGAGCCGGAGTAATGCCATCCGGTTTTGGGTCGGGTTCAGAGGAGGTTATGTACATGAGAGCTAATTATGAACACGTGGTCGGAAGTGCTGACTCGGAATCATTTCATCTTATTAACCCGGACGAGTGCCCGGGTCAAGAACTCAGTATATTCTTGCTCAGATCTCGAAATGGGTAA

mRNA sequence

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCCTGTTTCTTTCCCCCCACCGCCGCCACCGCCGCCGCCTCCTCCGCTGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACCTCGAAACCACCACCGCCACCCTCAATGAAGATCCAACCCATGGCCAAGATTCTCCCGCCACCACTCCCGACAGCGTCACACCCAAATTCGCTGTCTCTGCCTCCATTGTAGCCCCACCCCGTCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCATCGGAGAGGACACGTTTGGTTCTGTGTCCAACATGACCGTCTCCGTAACAAACCGTTTCTCCTACTCGAGTTTCCGATTCTGACTCACCAACTCGTGAATGAAATGCGATTCGGACTGGTTCGAATCGCTCTCGAGTGTAACCGGGTGGAACTGGGGTTCTGCCCACTTCGTTCGATTCCTATTTGGGCAATGTCATGTAATGGGCGAAAACTGGGGTTCGCGGCTAAGAAAAAAGCCGGTGAACCGGTCCGGTCCATGTTGAAGACAATGCAATCGACAACGGTGGGAGCCGGAGTAATGCCATCCGGTTTTGGGTCGGGTTCAGAGGAGGTTATGTACATGAGAGCTAATTATGAACACGTGGTCGGAAGTGCTGACTCGGAATCATTTCATCTTATTAACCCGGACGAGTGCCCGGGTCAAGAACTCAGTATATTCTTGCTCAGATCTCGAAATGGGTAA

Coding sequence (CDS)

ATGGCCAAGATCGACGCCCTTCGTCGGTTTCTCCTCCCCTGTTTCTTTCCCCCCACCGCCGCCACCGCCGCCGCCTCCTCCGCTGTTCCCAAGAAACGTCTAAGCACCTCGCTTCGCGACGACCTCGAAACCACCACCGCCACCCTCAATGAAGATCCAACCCATGGCCAAGATTCTCCCGCCACCACTCCCGACAGCGTCACACCCAAATTCGCTGTCTCTGCCTCCATTGTAGCCCCACCCCGTCCTTCGAAAACCATGGTCATCGGAACCATCTTCGGCCATCGGAGAGGACACGTTTGGTTCTGTGTCCAACATGACCGTCTCCGTAACAAACCGTTTCTCCTACTCGAGTTTCCGATTCTGACTCACCAACTCGTGAATGAAATGCGATTCGGACTGGTTCGAATCGCTCTCGAGTGTAACCGGGTGGAACTGGGGTTCTGCCCACTTCGTTCGATTCCTATTTGGGCAATGTCATGTAATGGGCGAAAACTGGGGTTCGCGGCTAAGAAAAAAGCCGGTGAACCGGTCCGGTCCATGTTGAAGACAATGCAATCGACAACGGTGGGAGCCGGAGTAATGCCATCCGGTTTTGGGTCGGGTTCAGAGGAGGTTATGTACATGAGAGCTAATTATGAACACGTGGTCGGAAGTGCTGACTCGGAATCATTTCATCTTATTAACCCGGACGAGTGCCCGGGTCAAGAACTCAGTATATTCTTGCTCAGATCTCGAAATGGGTAA

Protein sequence

MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSIFLLRSRNG*

Homology

[Close]

BLAST of CSPI04G15140 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-32
Identity = 97/250 (38.80%), Postives = 137/250 (54.80%), Query Frame = 0


Query: 10  FLLP---CFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSPATTPDS 69
           FL+P   C + P +++++AS  + K  L  SL   L +    +N         P+    S
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLL--LRSFINIINIPACKMLSLPSPPSSS 109

Query: 70  VTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQL 129
            +    + + +          V GT++GH+RGHV F VQ+++ R+ P LLL+  + T  L
Sbjct: 110 SSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATL 169

Query: 130 VNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG--EPVRSMLKT 189
           V EM  GLVRIALEC +       L   P W M CNGRK G+A  +     +    +L T
Sbjct: 170 VKEMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNT 229

Query: 190 MQSTTVGAGVMP--------SGFGSGSE--EVMYMRANYEHVVGSADSESFHLINPDECP 245
           +   TVGAGV+P        SG GSG+E  E++YMR  +E VVGS DSE+F+++NPD+  
Sbjct: 230 VSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNG 289

[Close]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL
Match: A0A0A0KXC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 7.6e-138
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60

Query: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120
           ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP
Sbjct: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120

Query: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180
           ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS
Sbjct: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180

Query: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240
           MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI
Sbjct: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240

Query: 241 FLLRSRNG 249
           FLLRSRNG
Sbjct: 241 FLLRSRNG 248

[Close]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL
Match: A0A5D3DK02 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001000 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.1e-134
Identity = 241/248 (97.18%), Postives = 244/248 (98.39%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRRFLLPCFFPPTAATAAASS VPKKRLSTSLRDDLETTTAT++EDPTH QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQDSP 60

Query: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120
           ATTPDSVTPKFA+SASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP
Sbjct: 61  ATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120

Query: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180
           ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGE VRS
Sbjct: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGESVRS 180

Query: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240
           MLKTMQSTTVGAGVMPSG GSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI
Sbjct: 181 MLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240

Query: 241 FLLRSRNG 249
           FLLRSRNG
Sbjct: 241 FLLRSRNG 248

[Close]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL
Match: A0A1S3BKH4 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.1e-134
Identity = 241/248 (97.18%), Postives = 244/248 (98.39%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRRFLLPCFFPPTAATAAASS VPKKRLSTSLRDDLETTTAT++EDPTH QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQDSP 60

Query: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120
           ATTPDSVTPKFA+SASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP
Sbjct: 61  ATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120

Query: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180
           ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGE VRS
Sbjct: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGESVRS 180

Query: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240
           MLKTMQSTTVGAGVMPSG GSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI
Sbjct: 181 MLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240

Query: 241 FLLRSRNG 249
           FLLRSRNG
Sbjct: 241 FLLRSRNG 248

[Close]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL
Match: A0A6J1ISJ8 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.1e-119
Identity = 222/252 (88.10%), Postives = 229/252 (90.87%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLE-TTTATLNEDPTHGQDS 60
           MAKIDALRRF LPCFFPPTA TA A+SA PKKRLSTSLRDDLE + + T N  PTH QDS
Sbjct: 1   MAKIDALRRFFLPCFFPPTATTATAASAAPKKRLSTSLRDDLEISASTTANGGPTHDQDS 60

Query: 61  PATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120
           PATTPDSVTPKF  +A+IVAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRNKPFLLLEF
Sbjct: 61  PATTPDSVTPKFVTAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFLLLEF 120

Query: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVR 180
           PILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG+ +R
Sbjct: 121 PILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAGDSIR 180

Query: 181 SMLKTMQSTTVGAGVMPSGFGSGS----EEVMYMRANYEHVVGSADSESFHLINPDECPG 240
           SMLKTMQSTTVGAGVMPSGFG GS    EEVMYMRANYEHVVGSADSESFHLINPDE PG
Sbjct: 181 SMLKTMQSTTVGAGVMPSGFGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPDEFPG 240

Query: 241 QELSIFLLRSRN 248
           QELSIFLLRS N
Sbjct: 241 QELSIFLLRSPN 252

[Close]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL
Match: A0A6J1F0T8 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.3e-116
Identity = 219/249 (87.95%), Postives = 229/249 (91.97%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRR+LLPCFFPPT ATA ASSA PKKRLSTSLRDD+ETTT   N  PT  QDSP
Sbjct: 1   MAKIDALRRYLLPCFFPPT-ATATASSAAPKKRLSTSLRDDVETTT---NGYPTDDQDSP 60

Query: 61  ATTPDSVTPKFAVSA-SIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120
           +TTPDSVTPKF  +A +IVAPPRPSKTMVIGTIFGHRRGH+WFCVQHDRLR KPFLLLE 
Sbjct: 61  STTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLEL 120

Query: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVR 180
           PILTHQLVNEMRFGLVRIALE N VELGFCPLRSIPIWAMSCNGRKLGFAA+KK G+PVR
Sbjct: 121 PILTHQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGGDPVR 180

Query: 181 SMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELS 240
           SMLKTMQSTTVGAGV+PSGFGS SEE+MYMRANYEHVVGSADSESFHLINPDE PGQELS
Sbjct: 181 SMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELS 240

Query: 241 IFLLRSRNG 249
           IFLLRSRNG
Sbjct: 241 IFLLRSRNG 245

[Close]

BLAST of CSPI04G15140 vs. NCBI nr
Match: XP_004142185.2 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017911 [Cucumis sativus])

HSP 1 Score: 499.6 bits (1285), Expect = 1.6e-137
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60

Query: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120
           ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP
Sbjct: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120

Query: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180
           ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS
Sbjct: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180

Query: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240
           MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI
Sbjct: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240

Query: 241 FLLRSRNG 249
           FLLRSRNG
Sbjct: 241 FLLRSRNG 248

[Close]

BLAST of CSPI04G15140 vs. NCBI nr
Match: XP_008448402.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK23589.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.1e-133
Identity = 241/248 (97.18%), Postives = 244/248 (98.39%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60
           MAKIDALRRFLLPCFFPPTAATAAASS VPKKRLSTSLRDDLETTTAT++EDPTH QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQDSP 60

Query: 61  ATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120
           ATTPDSVTPKFA+SASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP
Sbjct: 61  ATTPDSVTPKFAISASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFP 120

Query: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRS 180
           ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGE VRS
Sbjct: 121 ILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGESVRS 180

Query: 181 MLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240
           MLKTMQSTTVGAGVMPSG GSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI
Sbjct: 181 MLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSI 240

Query: 241 FLLRSRNG 249
           FLLRSRNG
Sbjct: 241 FLLRSRNG 248

[Close]

BLAST of CSPI04G15140 vs. NCBI nr
Match: XP_038880832.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 455.3 bits (1170), Expect = 3.4e-124
Identity = 228/253 (90.12%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTA-TLNEDPTH---- 60
           MAKID+LRRFLLPCFFPPT   A A+SAVPKKRLSTSLRDD+ETTT+ T N DPT+    
Sbjct: 1   MAKIDSLRRFLLPCFFPPTVTNATAASAVPKKRLSTSLRDDVETTTSTTANGDPTNDQDS 60

Query: 61  GQDSPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
            QDSPATTPD+VTPKFA +A+I APPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL
Sbjct: 61  AQDSPATTPDNVTPKFAATAAIAAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLEFPILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240
           + VRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG
Sbjct: 181 DSVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPG 240

Query: 241 QELSIFLLRSRNG 249
           QELSIFLLRSRNG
Sbjct: 241 QELSIFLLRSRNG 253

[Close]

BLAST of CSPI04G15140 vs. NCBI nr
Match: XP_022978268.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 438.3 bits (1126), Expect = 4.3e-119
Identity = 222/252 (88.10%), Postives = 229/252 (90.87%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLE-TTTATLNEDPTHGQDS 60
           MAKIDALRRF LPCFFPPTA TA A+SA PKKRLSTSLRDDLE + + T N  PTH QDS
Sbjct: 1   MAKIDALRRFFLPCFFPPTATTATAASAAPKKRLSTSLRDDLEISASTTANGGPTHDQDS 60

Query: 61  PATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120
           PATTPDSVTPKF  +A+IVAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRNKPFLLLEF
Sbjct: 61  PATTPDSVTPKFVTAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFLLLEF 120

Query: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVR 180
           PILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG+ +R
Sbjct: 121 PILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAGDSIR 180

Query: 181 SMLKTMQSTTVGAGVMPSGFGSGS----EEVMYMRANYEHVVGSADSESFHLINPDECPG 240
           SMLKTMQSTTVGAGVMPSGFG GS    EEVMYMRANYEHVVGSADSESFHLINPDE PG
Sbjct: 181 SMLKTMQSTTVGAGVMPSGFGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPDEFPG 240

Query: 241 QELSIFLLRSRN 248
           QELSIFLLRS N
Sbjct: 241 QELSIFLLRSPN 252

[Close]

BLAST of CSPI04G15140 vs. NCBI nr
Match: KAG7033814.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434.1 bits (1115), Expect = 8.1e-118
Identity = 222/254 (87.40%), Postives = 230/254 (90.55%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLE-TTTATLNEDPTHGQDS 60
           M KIDALRRF LPCFFPPTA TA A++A PKKRLSTSLRDDLE +T+ T N  PTH QDS
Sbjct: 1   MTKIDALRRFFLPCFFPPTATTATAATAAPKKRLSTSLRDDLEISTSTTANGGPTHDQDS 60

Query: 61  PATTPDSVTPKF----AVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           PATTPDSVTPKF    A +A+IVAPPRPSKTMVIGTIFGHRRGHVWFCVQ DRLRNKPFL
Sbjct: 61  PATTPDSVTPKFAAAAAAAAAIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG 180
           LLE PILTHQLVNEMRFGLVRIALECNRVELG CPLRSIPIWAMSCNGRKLGFAAKKKAG
Sbjct: 121 LLELPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAG 180

Query: 181 EPVRSMLKTMQSTTVGAGVMPSGFGSGS--EEVMYMRANYEHVVGSADSESFHLINPDEC 240
           + +RSMLKTMQSTTVGAGVMPSGFGSGS  EEVMYMRANYEHVVGSADSESFHLINPDE 
Sbjct: 181 DSIRSMLKTMQSTTVGAGVMPSGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPDEF 240

Query: 241 PGQELSIFLLRSRN 248
           PGQELSIFLLRS N
Sbjct: 241 PGQELSIFLLRSPN 254

[Close]

BLAST of CSPI04G15140 vs. TAIR 10
Match: AT2G37880.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 277.3 bits (708), Expect = 1.2e-74
Identity = 156/257 (60.70%), Postives = 178/257 (69.26%), Query Frame = 0


Query: 1   MAKIDALRRFLLPCFFPPTAAT--AAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQD 60
           M KID+LRRFLLPC   PT  T  +  S+   KKRLSTSLRDD++             QD
Sbjct: 1   MTKIDSLRRFLLPCISTPTNPTTVSTTSTGASKKRLSTSLRDDIDV------------QD 60

Query: 61  SPATTPDSVTPKFAVSASIVA---PPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFL 120
           S +++  S     AV  SI A   P RPSKTMVIGTIFG R+GHVWFCVQHDRL  KP L
Sbjct: 61  SASSSASSSEATSAVDYSISAVTVPQRPSKTMVIGTIFGRRKGHVWFCVQHDRLSVKPIL 120

Query: 121 LLEFPILTHQLVNEMRFGLVRIALEC-NRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKA 180
           LLE  I T QLV+EM  GLVR+ALEC  R EL  C LRS+P+W M CNGRKLGFA ++ A
Sbjct: 121 LLELSIATSQLVHEMGSGLVRVALECPTRPELKSCLLRSVPVWTMFCNGRKLGFAVRRSA 180

Query: 181 GEPVRSMLKTMQSTTVGAGVMPSGFGSG------SEEVMYMRANYEHVVGSADSESFHLI 240
            E  R MLK ++S TVGAGV+PSG G G      ++EVMYMRANYEHVVGS+DSESFHLI
Sbjct: 181 NEETRMMLKRLESMTVGAGVLPSGSGLGGSDESDTDEVMYMRANYEHVVGSSDSESFHLI 240

Query: 241 NPDECPGQELSIFLLRS 246
           NPD    QELSIFLLR+
Sbjct: 241 NPDANSAQELSIFLLRT 245

[Close]

BLAST of CSPI04G15140 vs. TAIR 10
Match: AT1G21050.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 154.1 bits (388), Expect = 1.5e-37
Identity = 83/166 (50.00%), Postives = 108/166 (65.06%), Query Frame = 0


Query: 84  SKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEM-RFGLVRIALECN 143
           S + V GT FGHRRG V FC+Q   + + P LLLE  + T  L  EM   G++RIALEC+
Sbjct: 83  SSSTVSGTFFGHRRGRVSFCLQDAAVGSSPLLLLELAVPTAALAKEMDEEGVLRIALECD 142

Query: 144 RVELGFCPLRSI---PIWAMSCNGRKLGFAAKKKAGEPVRSMLKTMQSTTVGAGVMPSGF 203
           R         SI   P+W+M CNGRK+GFA ++K  E     L+ MQS +VGAGV+PS  
Sbjct: 143 RRRSSNSRSSSIFDVPVWSMFCNGRKMGFAVRRKVTENDAVFLRMMQSVSVGAGVVPS-- 202

Query: 204 GSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELSIFLLRS 246
               ++++Y+RA +E V GS+DSESFH++NP    GQELSIFLLRS
Sbjct: 203 -EEEDQMLYLRARFERVTGSSDSESFHMMNPGGSYGQELSIFLLRS 245

[Close]

BLAST of CSPI04G15140 vs. TAIR 10
Match: AT1G76610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-34
Identity = 86/192 (44.79%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 57  QDSPATTPDSVTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHD-RLRNKPFL 116
           Q  P    D+V+   + S+S       +  +V GT +GHRRGHV FC+Q D R  + P L
Sbjct: 50  QPPPTDQNDTVSVSSSTSSS-----DQTGVVVTGTFYGHRRGHVSFCLQDDTRPSSPPLL 109

Query: 117 LLEFPILTHQLVNEMRFGLVRIAL--ECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKK 176
           LLE  + T  L  EM  G +RIAL  + NR    F    ++P+W+M CNGRK GFA +++
Sbjct: 110 LLELAVPTAALAREMEEGFLRIALRSKSNRRSSIF----NVPVWSMYCNGRKFGFAVRRE 169

Query: 177 AGEPVRSMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDEC 236
             E     L+ MQS +VGAGV+P+G      E +Y+RA +E V GS+DSESFH++N    
Sbjct: 170 TTENDVGFLRLMQSVSVGAGVIPNG------ETLYLRAKFERVTGSSDSESFHMVNQGGG 226

Query: 237 PGQELSIFLLRS 246
            GQELSIFL RS
Sbjct: 230 YGQELSIFLSRS 226

[Close]

BLAST of CSPI04G15140 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 141.7 bits (356), Expect = 7.7e-34
Identity = 97/250 (38.80%), Postives = 137/250 (54.80%), Query Frame = 0


Query: 10  FLLP---CFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSPATTPDS 69
           FL+P   C + P +++++AS  + K  L  SL   L +    +N         P+    S
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLL--LRSFINIINIPACKMLSLPSPPSSS 109

Query: 70  VTPKFAVSASIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQL 129
            +    + + +          V GT++GH+RGHV F VQ+++ R+ P LLL+  + T  L
Sbjct: 110 SSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATL 169

Query: 130 VNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAG--EPVRSMLKT 189
           V EM  GLVRIALEC +       L   P W M CNGRK G+A  +     +    +L T
Sbjct: 170 VKEMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNT 229

Query: 190 MQSTTVGAGVMP--------SGFGSGSE--EVMYMRANYEHVVGSADSESFHLINPDECP 245
           +   TVGAGV+P        SG GSG+E  E++YMR  +E VVGS DSE+F+++NPD+  
Sbjct: 230 VSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNG 289

[Close]

BLAST of CSPI04G15140 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 132.1 bits (331), Expect = 6.1e-31
Identity = 77/170 (45.29%), Postives = 102/170 (60.00%), Query Frame = 0


Query: 88  VIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEFPILTHQLVNEMRFGLVRIALECNRVEL- 147
           V+GT+FG+RRGHV+F VQ D  R  P +L++ P  T  LV EM  GLVRIALE    +  
Sbjct: 98  VVGTLFGNRRGHVYFAVQDDPTR-LPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 157

Query: 148 GFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVRSMLKTMQSTTVGAGVMPS--------- 207
               L     W   CNG+K G+AA+K+ GE    +LK +   T+GAGV+P+         
Sbjct: 158 SKKKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEG 217

Query: 208 --GFGSGSEEVMYMRANYEHVVGSADSESFHLINPD-ECPGQELSIFLLR 245
               GS   E+MYMRA +E VVGS DSE+F+++NPD    G ELS++ LR
Sbjct: 218 NGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLR 266

The following BLAST results are available for this feature:

BLAST of CSPI04G15140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	1.1e-32	38.80	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of CSPI04G15140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXC2	7.6e-138	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1	[more]
A0A5D3DK02	5.1e-134	97.18	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50...	[more]
A0A1S3BKH4	5.1e-134	97.18	protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103490601 PE=4 SV=1	[more]
A0A6J1ISJ8	2.1e-119	88.10	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1	[more]
A0A6J1F0T8	1.3e-116	87.95	protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1	[more]

BLAST of CSPI04G15140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142185.2	1.6e-137	100.00	protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017...	[more]
XP_008448402.1	1.1e-133	97.18	PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0045148.1 protein MIZU-KUSSE...	[more]
XP_038880832.1	3.4e-124	90.12	protein MIZU-KUSSEI 1 [Benincasa hispida]	[more]
XP_022978268.1	4.3e-119	88.10	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
KAG7033814.1	8.1e-118	87.40	Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI04G15140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37880.1	1.2e-74	60.70	Protein of unknown function, DUF617	[more]
AT1G21050.1	1.5e-37	50.00	Protein of unknown function, DUF617	[more]
AT1G76610.1	1.2e-34	44.79	Protein of unknown function, DUF617	[more]
AT2G41660.1	7.7e-34	38.80	Protein of unknown function, DUF617	[more]
AT3G25640.1	6.1e-31	45.29	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 88..244 e-value: 1.4E-58 score: 197.5
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 88..245 e-value: 2.1E-58 score: 194.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..67
None	No IPR available	PANTHER	PTHR31276	FAMILY NOT NAMED	coord: 1..246
None	No IPR available	PANTHER	PTHR31276:SF6	PROTEIN MIZU-KUSSEI 1	coord: 1..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G15140.1	CSPI04G15140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism