CSPI04G15130 (gene) Cucumber (PI 183967) v1

BLAST of CSPI04G15130 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 560.5 bits (1443), Expect = 2.3e-158
Identity = 283/537 (52.70%), Postives = 377/537 (70.20%), Query Frame = 0

Query: 17  KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 76
           K+ +GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 77  PLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGESNCPQPLPWQ 136
            LAGAF++DAYLGRF+TLL GS+AS +GMG+  L A LP +RP  CI   +   QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 137 LGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISLVIAL 196
           LG L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F+++LVIAL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 197 TIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRK 256
           T VVY+QTNISW +GF IPTAC   SI  F++G   YIC   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 257 WEIPKTTKFDQLHNPPMNSSK---LAHTNRFLIFDKAATVVDSSVELDEEGKSKNEWRLC 316
            ++   +       P  + S    +   +R   FDKA+ V + + EL+E+G +K +WRLC
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPN-ELNEDGNAKYKWRLC 314

Query: 317 SVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPH-FQIPPAWMSL 376
           SV QV+  KCV  I PVW+ GI CF+   Q + +GILQA+QM+++ GPH FQ+P  WM+L
Sbjct: 315 SVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNL 374

Query: 377 TPMIALSIWIYIYE-KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFRRD 436
             MI L+IWI +YE   +  +K+ T   KR ++K RIE  IVM ++CM+VAG  EK RR 
Sbjct: 375 VSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRA 434

Query: 437 AAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRTVAGAIFFFSLS 496
           +A++N SF+SP+ + +L+P+FAL+GLTEAF A+A+ME LT  +PE +R VAGAIFF S S
Sbjct: 435 SALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSS 494

Query: 497 LASYLSSVLTGIVRGVERE----WLGGNDLNKNRLDYFFSVVGVIAALNFFYFRFFA 545
           +ASY+ ++L  ++  V R+    WLG  DLNKNRL+ +F ++  I   N  YFR FA
Sbjct: 495 IASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFA 548

BLAST of CSPI04G15130 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.5e-112
Identity = 215/549 (39.16%), Postives = 341/549 (62.11%), Query Frame = 0

Query: 11  LDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWG 70
           LDA + ++  GGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  IW 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 71  GTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGES-NC 130
           G TN  PL GA+++D Y+GRF T+ F S A+LLG+  +TL A  PQ+ P  C S +  +C
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSC 162

Query: 131 PQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFS 190
             P   Q+G L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F+
Sbjct: 163 GGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFT 222

Query: 191 ISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKV 250
           + L+I  T+VVY+Q  +SW +GF IPT     ++ +F  G + Y+   P+GS+F+ +A+V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 251 VVATCRKWEIPKTTKFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSVELDEE 310
           +VA  +K ++    + D     ++P + S   SKL  +N+F   DKAA V++   +L  E
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPE 342

Query: 311 GKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHF 370
           G   ++WRLCSV +VE+ KC++ I P+W AGI    +M    +F + QA++M+R++GP F
Sbjct: 343 GPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKF 402

Query: 371 QIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVA 430
           +IP   +S+  ++ + I++  Y++ +V  M++ T      ++  RI  GIV ++  M+VA
Sbjct: 403 EIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVA 462

Query: 431 GVLEKFRRDAAVE--NKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 490
           G++E+ RR  ++   + + ++P+ V+ L P+  L GL EAF  I  +E   +  PE +R+
Sbjct: 463 GIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRS 522

Query: 491 VAGAIFFFSLSLASYLSSVLTGIVR----GVER-EWLGGNDLNKNRLDYFFSVVGVIAAL 545
           +A ++F  S + +SYLSS L  +V     G +R +WL  N LN  +LDYF+ ++ V+  +
Sbjct: 523 IANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVV 582

BLAST of CSPI04G15130 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 404.4 bits (1038), Expect = 2.1e-111
Identity = 218/540 (40.37%), Postives = 336/540 (62.22%), Query Frame = 0

Query: 21  GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGE-SNCPQPLPWQLGF 140
           A ++DAY+GRF T+ + S+ S+LG+  +TL A LPQ+ PPPC +     C  P   QLG 
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISLVIALTIV 200
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ L+ + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRKWEI 260
           VY+QT +SW +GF IPT+    ++ +F +G R+Y+   P+GSVF+ +A+V+VA  +K ++
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKTTKFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSVELDEEGKSKNEWRLC 320
             +   D   + + PP+     SKL  T++F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHFQIPPAWMSLT 380
           S+ +VE+ KC++ + PVW AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYE----KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFR 440
             I + IW+ IYE     ++  M+K      R ++  R+ IGIV ++L M  AG +E  R
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRMRK-----FRVTLLQRMGIGIVFAILSMFTAGFVEGVR 435

Query: 441 RDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRTVAGAIFFFS 500
           R  A E    ++ + V+ L     L GL E+F  I ++E   +  PE +R++A ++F  S
Sbjct: 436 RTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLS 495

Query: 501 LSLASYLSSVLTGIVRGV-----EREWLGGNDLNKNRLDYFFSVVGVIAALNFFYFRFFA 545
            + A+YLSS+L   V  V       +WL   DL++ +LDYF+ ++ V+  +N  YF + A
Sbjct: 496 FAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 542

BLAST of CSPI04G15130 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.4e-99
Identity = 201/546 (36.81%), Postives = 317/546 (58.06%), Query Frame = 0

Query: 14  SRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 73
           ++ K    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT 
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 74  NFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPC-ISGESNCPQP 133
           NF     AFL D Y GR+ TL    +A  LG  V+ L A +PQ+ P  C  + +S C  P
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 134 LPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISL 193
              Q+ FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ + 
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 194 VIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVA 253
           +++LT+VVYVQ+N+SWT+G  IP      +  IF  G + Y+     GS  A +A+V+  
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 254 TCRKWEI-PKTTKFDQLHN---PPMNSSKLAHTNRFLIFDKAATVVDSSVELDEEGKSKN 313
             +K  + P    +  L+N   P   +SKL +T++F   DKAA ++    +L  +GK  +
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPAD 337

Query: 314 EWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP-HFQIPP 373
            W+LC++ QVE+ KC+V + P+W A    ++++ Q  ++ + QA+Q +R +G   F IP 
Sbjct: 338 PWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPA 397

Query: 374 AWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLE 433
           A   +  M  ++++I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E
Sbjct: 398 ATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVE 457

Query: 434 KFRRDAAV--------ENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESL 493
           + RR  A+          K  IS +    LIP+ +L+G+ EAF AI  ME      PE++
Sbjct: 458 ERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENM 517

Query: 494 RTVAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGN----DLNKNRLDYFFSVVGVIAA 541
           R+ AG+IF+    ++SYL S L   V    +   GGN    DLNK RLD F+ ++  I A
Sbjct: 518 RSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILA 577

BLAST of CSPI04G15130 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.5e-98
Identity = 194/545 (35.60%), Postives = 327/545 (60.00%), Query Frame = 0

Query: 9   ASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           +S D +  +    GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGESN 128
           W   TNFAP+ GAF++D+Y G+F T++FGS+A LLGM VLT  + +P +RPPPC + +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPC-TADQI 127

Query: 129 CPQPLPWQLGFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY 188
             Q +P+    LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y
Sbjct: 128 TGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYY 187

Query: 189 FLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFAD 248
              +I  ++++T+V+YVQ NISW +GF IPT    F++ +  +G RYY+   P+GSVF+ 
Sbjct: 188 TTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSG 247

Query: 249 MAKVVVATCRKWEIPKTTKFDQLHNP----PMNSSKLAHTNRFLIFDKAATVVDSSVELD 308
           + KV+VA  +K +   T+  D  H P     + S+KL  T++F   +KA  V+++    D
Sbjct: 248 VFKVLVAAYKKRKARFTSGID-YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----D 307

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI-G 368
           E G    EWR C+V Q+E  K ++ I P++ + I  F++M Q  +F + QA++M+    G
Sbjct: 308 EAG--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPG 367

Query: 369 PHFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 428
             + IPPA +++  ++ + IW+  YE   V H++  T+ N   S+  ++ IG + S+  M
Sbjct: 368 TSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTM 427

Query: 429 VVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLR 488
           +++G++E+ RRD ++        + V+ L P+  L G  + F  + + E     +P ++R
Sbjct: 428 LISGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 487

Query: 489 TVAGAIFFFSLSLASYLSSVLTGIVRGVE----REWLGGNDLNKNRLDYFFSVVGVIAAL 541
           ++  ++ +  LSLASYLSS +  IV  V     + WL  +D++K++LD F+  +  ++ L
Sbjct: 488 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTL 539

BLAST of CSPI04G15130 vs. ExPASy TrEMBL
Match: A0A0A0KZQ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 9.0e-259
Identity = 468/563 (83.13%), Postives = 470/563 (83.48%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGESNCPQPLPWQLGFLYLGLGLIVIGAG                            
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAG---------------------------- 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
               DMAKVVVATCRKW+IPKTT+FDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL
Sbjct: 241 ----DMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG
Sbjct: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT
Sbjct: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 471

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 540
           VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF
Sbjct: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 471

Query: 541 RFFAVAFLPKPDVDPSHKVHQMQ 564
           RFFAVAFLPKPDVDPSHK HQMQ
Sbjct: 541 RFFAVAFLPKPDVDPSHKAHQMQ 471

BLAST of CSPI04G15130 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.4e-257
Identity = 450/578 (77.85%), Postives = 499/578 (86.33%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+NG  L +SLD S  K   GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV
Sbjct: 1   MDNGT-LRSSLDPS-PKPQLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGS+AS LGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC SG+++C Q   WQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSI+LVIALT+VVY+QTNISWTLGF IPTA F+FSI IFLLG   YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
           SVFADMAKV++AT RK +IP+ +  + LH+PPM+SSKL HTNRFL+FDKAA V+DSS+EL
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+G+SKNEW+LC+VHQVEQ KCVVGI P+W AGI CF+SMQQM SFGILQAIQMNR +G
Sbjct: 301 DEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHF+IPPAWMSL PMIALS+WIYIYEKYVE M++KT  NKRFSMK RIEIGIVMSVLCM+
Sbjct: 361 PHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMI 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAG+LEK RRD+A+EN++F+SPLHV VLIPEFALSGLTEAF AI+VMELLTT LPESLRT
Sbjct: 421 VAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRT 480

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVER------EWLGGNDLNKNRLDYFFSVVGVIAA 540
           VAGAIFF SLSLASYLSSVL GIV  V +       WL GNDLNKN LDYFF VVGVIAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           LNFFYFRF    FLP+ DVDP  K  + + ED E G R
Sbjct: 541 LNFFYFRFVGTRFLPEGDVDPRVKASERRSEDEESGFR 575

BLAST of CSPI04G15130 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.7e-255
Identity = 439/468 (93.80%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGAD 164
           MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQL FLYLGLGLIVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIF 224
           QFDTNTEKGRAKLESFLNWWYFLFS++LVIALTIVVYVQTN+SWTLGFGIPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 225 IFLLGHRYYICKAPQGSVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRF 284
           IFLLGHRYYICKAPQGSVFAD+AKVVVATCRK +IPK T+FDQLHNPPMNSSKLAHTNRF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 285 LIFDKAATVVDSSVELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQM 344
           LIFDKAATVVDSSVELDE+GKSKNEWRLCSVHQVE+FKCVVGI PVWLAGIPCFMSMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMI LSIWIYIYEKYVEHMKKKT+SNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAI 464
           KTRIEIGIVMSVLCMVVAG+LEK RRDAAVENKSFISPLHVWVLIPEFALSGLTEAF AI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 465 AVMELLTTHLPESLRTVAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDY 524
           AVMELLTTHLPESLRTVAGAIFF SLSLASYLSSVLTGIV  VEREWLGGNDLNKNRLDY
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVEREWLGGNDLNKNRLDY 420

Query: 525 FFSVVGVIAALNFFYFRFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           FF VVGVIAALNFFYFRFFA AFLPKPDVDP  K HQ QLEDAELGRR
Sbjct: 421 FFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of CSPI04G15130 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 6.2e-244
Identity = 426/585 (72.82%), Postives = 478/585 (81.71%), Query Frame = 0

Query: 1   MENGNLLHASLD---ASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNL 60
           MEN  + H+SLD    + +K   GGWRAVRFIL NETFEKLASMSL+ NL+LYLHTMYNL
Sbjct: 1   MENDAIDHSSLDPPPVAPSKAAVGGWRAVRFILANETFEKLASMSLVANLILYLHTMYNL 60

Query: 61  DNVASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQM 120
           DNVASA V  IWGGTTNFAP+AGAFLADAYLGRFYTLLFGS+AS  GMGVLTLGAGLP++
Sbjct: 61  DNVASANVINIWGGTTNFAPIAGAFLADAYLGRFYTLLFGSIASFFGMGVLTLGAGLPEL 120

Query: 121 RPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKL 180
           RPP C   ++ CPQP PWQL FLYLGLGL+VIGAGGIRPCNISFGADQFDT T KGRAKL
Sbjct: 121 RPPSCSHSQAKCPQPQPWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTTTYKGRAKL 180

Query: 181 ESFLNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKA 240
           ESFLNWWYFLFSI+L++ALT+VVY+QTNISW  GF IPTACFV SI IFLLG   YI K 
Sbjct: 181 ESFLNWWYFLFSIALIVALTVVVYIQTNISWVTGFAIPTACFVLSILIFLLGRGTYIRKT 240

Query: 241 PQGSVFADMAKVVVATCRKWEIPKTTKFDQLHNPP---MNSSKLAHTNRFLIFDKAATVV 300
           PQGSVFADMAKV++ATCRK ++ K    + LH+PP    +S+KL HTNRFL+FDKAATVV
Sbjct: 241 PQGSVFADMAKVIIATCRKSKLSKPNNQNNLHDPPPTIPDSTKLPHTNRFLVFDKAATVV 300

Query: 301 DSSVELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQ 360
           D   ELDE G S + WRLCSV QVEQ KCV+GI PVW+AGI CF++MQQM SFG+LQAIQ
Sbjct: 301 DPVAELDEHGNSVSHWRLCSVQQVEQLKCVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQ 360

Query: 361 MNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVM 420
           M+R +G HF+IPPAWM+LTPMIALSIWI++YEKYV  MK+KT  NKR SM+ RIEIGIVM
Sbjct: 361 MDRFLGRHFEIPPAWMNLTPMIALSIWIFVYEKYVAMMKRKTGQNKRISMQRRIEIGIVM 420

Query: 421 SVLCMVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHL 480
           SV+CMVV+G+ EK RRD AV N SF++ LHV  L PEFALSGLTEAF AIAVMELLTTHL
Sbjct: 421 SVVCMVVSGITEKLRRDLAVGNGSFVARLHVTALXPEFALSGLTEAFAAIAVMELLTTHL 480

Query: 481 PESLRTVAGAIFFFSLSLASYLSSVLTGIVR-------GVEREWLGGNDLNKNRLDYFFS 540
           PESLRTVAGAIFF SLSLASYLSSVL GI+        G +  W+GGNDLN NRL+YFF 
Sbjct: 481 PESLRTVAGAIFFLSLSLASYLSSVLVGIISKISTTIDGKKTAWIGGNDLNHNRLEYFFY 540

Query: 541 VVGVIAALNFFYFRFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           VV VI  LNFFYFRFFA  FLPK DVD   + H+ QLED E GRR
Sbjct: 541 VVAVIGTLNFFYFRFFAAGFLPKSDVDDHDQTHERQLEDEETGRR 585

BLAST of CSPI04G15130 vs. ExPASy TrEMBL
Match: A0A6J1IS88 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111478233 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 3.3e-221
Identity = 381/455 (83.74%), Postives = 408/455 (89.67%), Query Frame = 0

Query: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGAD 164
           MGVLTLGAG P+MRPPPC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 165 QFDTNTEKGRAKLESFLNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIF 224
           QFDTNTEKGRAKLESFLNWWY LFS++LVIALT+VVY+QT+ISWTLGF IPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 225 IFLLGHRYYICKAPQGSVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRF 284
           IFLLG R+YICK PQGSVFADMAKV+VATCRK  +PK T+ D L++PP++SSKLAHT+RF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 285 LIFDKAATVVDSSVELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQM 344
           L+FDKAATV DSS ELDE+GKSKNEW+LCSVHQVEQ KCVVGI P+W AGIPCF+SMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT  NKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAI 464
           KTRIEIGIVMSVLCMVVAG+LE  RR +AVENK F+SPLHVW LIPEFALSGLTEAF AI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 465 AVMELLTTHLPESLRTVAGAIFFFSLSLASYLSSVLTGIVRGVER------EWLGGNDLN 524
           AVMELLTTHLPESLRTVAGAIFF SLSL SY+SSVL  IV  + R      EWLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 525 KNRLDYFFSVVGVIAALNFFYFRFFAVAFLPKPDV 554
           KNRLDYFF VVGVIAA NFFYFRFFA  FLPKP+V
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of CSPI04G15130 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 571/572 (99.83%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG
Sbjct: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
           SVFADMAKVVVATCRKW+IPKTT+FDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL
Sbjct: 241 SVFADMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG
Sbjct: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT
Sbjct: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 540
           VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF
Sbjct: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGNDLNKNRLDYFFSVVGVIAALNFFYF 540

Query: 541 RFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           RFFAVAFLPKPDVDPSHK HQMQLEDAELGRR
Sbjct: 541 RFFAVAFLPKPDVDPSHKAHQMQLEDAELGRR 572

BLAST of CSPI04G15130 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1005.4 bits (2598), Expect = 2.0e-289
Identity = 503/578 (87.02%), Postives = 526/578 (91.00%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+N N  H+S DA R K  RGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV
Sbjct: 1   MDNEN-RHSSSDAPRKKTARGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
           ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGS+AS LGMGVL+LGAGLPQMRPP
Sbjct: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PCISGES+CPQP PWQLGFLYLGLGLIV+GAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFSI+LVIALTIVVYVQTNISWTLGF IPTACFV SI IFL G R+Y+CKAPQG
Sbjct: 181 LNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
           SVF+D+AKVVVA  RK +IPK  + D LHNPPMNS KLAHT+RFL+FDKAATVVDSS+EL
Sbjct: 241 SVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRFLVFDKAATVVDSSIEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DEEGKSK+EWRLCSVHQVEQFKCVVGI PVWLAGI CF+SMQQM SFGILQAIQMNR IG
Sbjct: 301 DEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT SNKRFSMKTRIEIGIVMSVLCMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAG+LEK RRDAAVENKSF+SPLHVW+LIPEFALSGLTEAF AIAVMELLTTHLPESLRT
Sbjct: 421 VAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGV------EREWLGGNDLNKNRLDYFFSVVGVIAA 540
           VAGAIFF SLSLASYLSSVL GIV  +      E +WLGGNDLNKNRLDYFF VVG IAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAA 540

Query: 541 LNFFYFRFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           LNFFYFRFFA  FLP PDVDP  K HQ QLEDAELGRR
Sbjct: 541 LNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 577

BLAST of CSPI04G15130 vs. NCBI nr
Match: XP_023544027.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.5e-268
Identity = 467/559 (83.54%), Postives = 501/559 (89.62%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           ME+G  LH+S   +R K   GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V
Sbjct: 1   MESGR-LHSSF--NRPKAALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLLFGS ASLLGMGVLTLGAG P+MRPP
Sbjct: 61  DSAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFS++LVIALT+VVYVQT+ISWTLGF IPTACFVFSI IFLLG R+YICK PQG
Sbjct: 181 LNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
           SVFADMAKV+VATCRK  +PK T+ D LH+PPM+SSKL+HT+RFL+FDKAATVVDSS EL
Sbjct: 241 SVFADMAKVLVATCRKRNVPKFTQIDHLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+GKSKNEW+LCSVHQVEQFKCVVGI P+W+AGIPCF+SMQQM SFGILQAIQMNR IG
Sbjct: 301 DEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWVAGIPCFISMQQMGSFGILQAIQMNRFIG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHFQIPPAWMSLTPMIALSIWIYIYEK+VE MKKK   NKRFSMKTRIEIGIVMSV+CMV
Sbjct: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKFVEFMKKKNHKNKRFSMKTRIEIGIVMSVICMV 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAG+LE  RR++AVENKSF+SPLHVW+LIPEFALSGLTEAF AIAVMELLTTHLPESLRT
Sbjct: 421 VAGILENVRRNSAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRT 480

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVER------EWLGGNDLNKNRLDYFFSVVGVIAA 540
           VAGAIFF SLSL SY+SSVL  IV  + R       WLGGNDLNKNRLDYFF VVGVIAA
Sbjct: 481 VAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVAFLPKPDV 554
            NF YFRFFA  FLPKP+V
Sbjct: 541 FNFLYFRFFATHFLPKPNV 556

BLAST of CSPI04G15130 vs. NCBI nr
Match: KAG6603629.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.4 bits (2406), Expect = 3.7e-267
Identity = 468/560 (83.57%), Postives = 500/560 (89.29%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           ME+G  LH+S+  +R K   GGWRAVRFILGNETFEKLASMSLI NLVLYLHTMYNLD+V
Sbjct: 1   MESGR-LHSSV--NRPKIALGGWRAVRFILGNETFEKLASMSLIANLVLYLHTMYNLDSV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIW GTTNFA LAGAFLADAYLGRFYTLLFGS ASLLGMGVLTLGAG P+MRPP
Sbjct: 61  DSAYVFQIWSGTTNFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC  GESNCPQP PWQLGFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWY LFS++LVIALT+VVYVQT+ISWTLGF IPTACFVFSI IFLLG R+YICK PQG
Sbjct: 181 LNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQ-LHNPPMNSSKLAHTNRFLIFDKAATVVDSSVE 300
           SVFADMAKV+VATCRK  +PK T+ D  LH+PPM+SSKL+HT+RFL+FDKAATVVDSS E
Sbjct: 241 SVFADMAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTE 300

Query: 301 LDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI 360
           LDE+GKSKNEW+LCSVHQVEQFKC VGI P+W AGIPCF+SMQQM SFGILQAIQMNR I
Sbjct: 301 LDEQGKSKNEWKLCSVHQVEQFKCFVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFI 360

Query: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 420
           GPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT  NKRFS+KTRIEIGIVMSVLCM
Sbjct: 361 GPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSLKTRIEIGIVMSVLCM 420

Query: 421 VVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLR 480
           VVAG+LE  RR +A ENKSF+SPLHVW+LIPEFALSGLTEAF AIAVMELLTTHLPESLR
Sbjct: 421 VVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLR 480

Query: 481 TVAGAIFFFSLSLASYLSSVLTGIVRGVER------EWLGGNDLNKNRLDYFFSVVGVIA 540
           TVAGAIFF SLSL SY+SSVL  IV  + R       WLGGNDLNKNRLDYFF VVGVIA
Sbjct: 481 TVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIA 540

Query: 541 ALNFFYFRFFAVAFLPKPDV 554
           A NFFYFRFFA  FLPKP+V
Sbjct: 541 AFNFFYFRFFATHFLPKPNV 557

BLAST of CSPI04G15130 vs. NCBI nr
Match: XP_023518809.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 896.3 bits (2315), Expect = 1.3e-256
Identity = 451/578 (78.03%), Postives = 499/578 (86.33%), Query Frame = 0

Query: 1   MENGNLLHASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNV 60
           M+NG  L +SLD S  K   GGWRAVRFIL NETFEKLASMSLI NL+LYLHTMYNLDNV
Sbjct: 1   MDNGT-LRSSLDPS-PKPPLGGWRAVRFILANETFEKLASMSLIANLILYLHTMYNLDNV 60

Query: 61  ASAYVFQIWGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPP 120
            SAYVFQIWGGTTNF+PLAGAFLADAYLGRFYTLLFGS+AS LGMGVLTLGA LP+MRPP
Sbjct: 61  DSAYVFQIWGGTTNFSPLAGAFLADAYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPP 120

Query: 121 PCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180
           PC SG+++C Q   WQL FLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESF
Sbjct: 121 PCSSGKTDCIQSKSWQLAFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESF 180

Query: 181 LNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQG 240
           LNWWYFLFSI+LVIALT+VVY+QTNISWTLGF IPTA F+FSI IFLLG   YIC+ PQG
Sbjct: 181 LNWWYFLFSIALVIALTLVVYIQTNISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQG 240

Query: 241 SVFADMAKVVVATCRKWEIPKTTKFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVEL 300
           SVFADMAKV++AT RK +IP+ +  + LH+PPM+SSKL HTNRFL+FDKAA V+DSS+EL
Sbjct: 241 SVFADMAKVLIATYRKRQIPRPSP-NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMEL 300

Query: 301 DEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIG 360
           DE+G+SKNEW+LC+VHQVEQ KCVVGI P+W AGI CF+SMQQM SFGILQAIQMNR +G
Sbjct: 301 DEQGRSKNEWKLCTVHQVEQLKCVVGIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLG 360

Query: 361 PHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMV 420
           PHF+IPPAWMSL PMIALS+WIYIYEKYVE MK+KT  NKRFSMK RIEIGIVMSVLCMV
Sbjct: 361 PHFEIPPAWMSLIPMIALSLWIYIYEKYVEFMKRKTHHNKRFSMKMRIEIGIVMSVLCMV 420

Query: 421 VAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 480
           VAG+LEK RRD+A+EN++F+SPLHV VLIPEFALSGLTEAF AI+VMELLTT LPESLRT
Sbjct: 421 VAGILEKIRRDSAIENRTFVSPLHVSVLIPEFALSGLTEAFAAISVMELLTTQLPESLRT 480

Query: 481 VAGAIFFFSLSLASYLSSVLTGIVRGVER------EWLGGNDLNKNRLDYFFSVVGVIAA 540
           VAGAIFF SLSLASYLSSVL GIV  V +       WL GNDLNKN LDYFF VVGVIAA
Sbjct: 481 VAGAIFFLSLSLASYLSSVLMGIVNRVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAA 540

Query: 541 LNFFYFRFFAVAFLPKPDVDPSHKVHQMQLEDAELGRR 573
           LNFFYFRF    FLP+ DVDP  K  + + +D E G R
Sbjct: 541 LNFFYFRFVGTRFLPEGDVDPRGKAGERRSDDEESGFR 575

BLAST of CSPI04G15130 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 560.5 bits (1443), Expect = 1.6e-159
Identity = 283/537 (52.70%), Postives = 377/537 (70.20%), Query Frame = 0

Query: 17  KETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFA 76
           K+ +GGWRA+++I+ NE+FEKLASMSLIGNL +YL T YNL  V    V  IW G+ N  
Sbjct: 15  KKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNIL 74

Query: 77  PLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGESNCPQPLPWQ 136
            LAGAF++DAYLGRF+TLL GS+AS +GMG+  L A LP +RP  CI   +   QP  WQ
Sbjct: 75  TLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQ 134

Query: 137 LGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISLVIAL 196
           LG L+ GLGL+ IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWYF F+++LVIAL
Sbjct: 135 LGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIAL 194

Query: 197 TIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRK 256
           T VVY+QTNISW +GF IPTAC   SI  F++G   YIC   +GSVFAD+ KVV A C+K
Sbjct: 195 TGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKK 254

Query: 257 WEIPKTTKFDQLHNPPMNSSK---LAHTNRFLIFDKAATVVDSSVELDEEGKSKNEWRLC 316
            ++   +       P  + S    +   +R   FDKA+ V + + EL+E+G +K +WRLC
Sbjct: 255 RKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPN-ELNEDGNAKYKWRLC 314

Query: 317 SVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPH-FQIPPAWMSL 376
           SV QV+  KCV  I PVW+ GI CF+   Q + +GILQA+QM+++ GPH FQ+P  WM+L
Sbjct: 315 SVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNL 374

Query: 377 TPMIALSIWIYIYE-KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFRRD 436
             MI L+IWI +YE   +  +K+ T   KR ++K RIE  IVM ++CM+VAG  EK RR 
Sbjct: 375 VSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRA 434

Query: 437 AAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRTVAGAIFFFSLS 496
           +A++N SF+SP+ + +L+P+FAL+GLTEAF A+A+ME LT  +PE +R VAGAIFF S S
Sbjct: 435 SALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSS 494

Query: 497 LASYLSSVLTGIVRGVERE----WLGGNDLNKNRLDYFFSVVGVIAALNFFYFRFFA 545
           +ASY+ ++L  ++  V R+    WLG  DLNKNRL+ +F ++  I   N  YFR FA
Sbjct: 495 IASYICTLLINVIDAVTRKEGKSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFA 548

BLAST of CSPI04G15130 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 407.5 bits (1046), Expect = 1.7e-113
Identity = 215/549 (39.16%), Postives = 341/549 (62.11%), Query Frame = 0

Query: 11  LDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWG 70
           LDA + ++  GGWRAV FILGNET E+L S+ L+ N ++YL  +++L+ V +A V  IW 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 71  GTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGES-NC 130
           G TN  PL GA+++D Y+GRF T+ F S A+LLG+  +TL A  PQ+ P  C S +  +C
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSC 162

Query: 131 PQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFS 190
             P   Q+G L LGL  + +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y  F+
Sbjct: 163 GGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFT 222

Query: 191 ISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKV 250
           + L+I  T+VVY+Q  +SW +GF IPT     ++ +F  G + Y+   P+GS+F+ +A+V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 251 VVATCRKWEIPKTTKFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSVELDEE 310
           +VA  +K ++    + D     ++P + S   SKL  +N+F   DKAA V++   +L  E
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPE 342

Query: 311 GKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHF 370
           G   ++WRLCSV +VE+ KC++ I P+W AGI    +M    +F + QA++M+R++GP F
Sbjct: 343 GPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKF 402

Query: 371 QIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVA 430
           +IP   +S+  ++ + I++  Y++ +V  M++ T      ++  RI  GIV ++  M+VA
Sbjct: 403 EIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVA 462

Query: 431 GVLEKFRRDAAVE--NKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRT 490
           G++E+ RR  ++   + + ++P+ V+ L P+  L GL EAF  I  +E   +  PE +R+
Sbjct: 463 GIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRS 522

Query: 491 VAGAIFFFSLSLASYLSSVLTGIVR----GVER-EWLGGNDLNKNRLDYFFSVVGVIAAL 545
           +A ++F  S + +SYLSS L  +V     G +R +WL  N LN  +LDYF+ ++ V+  +
Sbjct: 523 IANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVV 582

BLAST of CSPI04G15130 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 404.4 bits (1038), Expect = 1.5e-112
Identity = 218/540 (40.37%), Postives = 336/540 (62.22%), Query Frame = 0

Query: 21  GGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFAPLAG 80
           GGWRA+ FILGNET EKL S+ +  N +LYL  +++++ V +  V+ +W G TNFAPL G
Sbjct: 16  GGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLG 75

Query: 81  AFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGE-SNCPQPLPWQLGF 140
           A ++DAY+GRF T+ + S+ S+LG+  +TL A LPQ+ PPPC +     C  P   QLG 
Sbjct: 76  ALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGI 135

Query: 141 LYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISLVIALTIV 200
           L+LGLG + IG+GGIRPC+I FG DQFD  TE+G   + SF NW+Y   ++ L+ + T+V
Sbjct: 136 LFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVV 195

Query: 201 VYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVATCRKWEI 260
           VY+QT +SW +GF IPT+    ++ +F +G R+Y+   P+GSVF+ +A+V+VA  +K ++
Sbjct: 196 VYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL 255

Query: 261 PKTTKFD---QLHNPPMNS---SKLAHTNRFLIFDKAATVVDSSVELDEEGKSKNEWRLC 320
             +   D   + + PP+     SKL  T++F   DKAA ++D   +L  EG   N+WRLC
Sbjct: 256 KISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLC 315

Query: 321 SVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHFQIPPAWMSLT 380
           S+ +VE+ KC++ + PVW AGI   ++M   ++F + QA +M+R +GPHF+IP A +++ 
Sbjct: 316 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVI 375

Query: 381 PMIALSIWIYIYE----KYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFR 440
             I + IW+ IYE     ++  M+K      R ++  R+ IGIV ++L M  AG +E  R
Sbjct: 376 SYITIGIWVPIYEHLLVPFLWRMRK-----FRVTLLQRMGIGIVFAILSMFTAGFVEGVR 435

Query: 441 RDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRTVAGAIFFFS 500
           R  A E    ++ + V+ L     L GL E+F  I ++E   +  PE +R++A ++F  S
Sbjct: 436 RTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLS 495

Query: 501 LSLASYLSSVLTGIVRGV-----EREWLGGNDLNKNRLDYFFSVVGVIAALNFFYFRFFA 545
            + A+YLSS+L   V  V       +WL   DL++ +LDYF+ ++ V+  +N  YF + A
Sbjct: 496 FAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 542

BLAST of CSPI04G15130 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.7e-100
Identity = 201/546 (36.81%), Postives = 317/546 (58.06%), Query Frame = 0

Query: 14  SRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 73
           ++ K    GW+ + FI+GNETFEKL  +  + NL++YL  ++NL ++ +A +   + GT 
Sbjct: 38  NQKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTI 97

Query: 74  NFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPC-ISGESNCPQP 133
           NF     AFL D Y GR+ TL    +A  LG  V+ L A +PQ+ P  C  + +S C  P
Sbjct: 98  NFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGP 157

Query: 134 LPWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISL 193
              Q+ FL +GLG +V+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++F F+ + 
Sbjct: 158 SGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQ 217

Query: 194 VIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVA 253
           +++LT+VVYVQ+N+SWT+G  IP      +  IF  G + Y+     GS  A +A+V+  
Sbjct: 218 ILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAV 277

Query: 254 TCRKWEI-PKTTKFDQLHN---PPMNSSKLAHTNRFLIFDKAATVVDSSVELDEEGKSKN 313
             +K  + P    +  L+N   P   +SKL +T++F   DKAA ++    +L  +GK  +
Sbjct: 278 AIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPAD 337

Query: 314 EWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGP-HFQIPP 373
            W+LC++ QVE+ KC+V + P+W A    ++++ Q  ++ + QA+Q +R +G   F IP 
Sbjct: 338 PWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPA 397

Query: 374 AWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLE 433
           A   +  M  ++++I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E
Sbjct: 398 ATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVE 457

Query: 434 KFRRDAAV--------ENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESL 493
           + RR  A+          K  IS +    LIP+ +L+G+ EAF AI  ME      PE++
Sbjct: 458 ERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENM 517

Query: 494 RTVAGAIFFFSLSLASYLSSVLTGIVRGVEREWLGGN----DLNKNRLDYFFSVVGVIAA 541
           R+ AG+IF+    ++SYL S L   V    +   GGN    DLNK RLD F+ ++  I A
Sbjct: 518 RSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILA 577

BLAST of CSPI04G15130 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-99
Identity = 194/545 (35.60%), Postives = 327/545 (60.00%), Query Frame = 0

Query: 9   ASLDASRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQI 68
           +S D +  +    GW+A+ +I+GNET E+LA+  L+ N ++Y+   Y++D V +  +   
Sbjct: 8   SSSDLTTRQRKPLGWKAMPYIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINT 67

Query: 69  WGGTTNFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGESN 128
           W   TNFAP+ GAF++D+Y G+F T++FGS+A LLGM VLT  + +P +RPPPC + +  
Sbjct: 68  WSALTNFAPIIGAFISDSYTGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPC-TADQI 127

Query: 129 CPQPLPWQLGFLYL---GLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY 188
             Q +P+    LY+   GL L+ +G GGIR C++ F  DQFD +TE+GR    SF +W+Y
Sbjct: 128 TGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYY 187

Query: 189 FLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFAD 248
              +I  ++++T+V+YVQ NISW +GF IPT    F++ +  +G RYY+   P+GSVF+ 
Sbjct: 188 TTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSG 247

Query: 249 MAKVVVATCRKWEIPKTTKFDQLHNP----PMNSSKLAHTNRFLIFDKAATVVDSSVELD 308
           + KV+VA  +K +   T+  D  H P     + S+KL  T++F   +KA  V+++    D
Sbjct: 248 VFKVLVAAYKKRKARFTSGID-YHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----D 307

Query: 309 EEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSI-G 368
           E G    EWR C+V Q+E  K ++ I P++ + I  F++M Q  +F + QA++M+    G
Sbjct: 308 EAG--NEEWRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPG 367

Query: 369 PHFQIPPAWMSLTPMIALSIWIYIYEK-YVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCM 428
             + IPPA +++  ++ + IW+  YE   V H++  T+ N   S+  ++ IG + S+  M
Sbjct: 368 TSYLIPPASITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTM 427

Query: 429 VVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLR 488
           +++G++E+ RRD ++        + V+ L P+  L G  + F  + + E     +P ++R
Sbjct: 428 LISGIVERKRRDLSLNGVK----MSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMR 487

Query: 489 TVAGAIFFFSLSLASYLSSVLTGIVRGVE----REWLGGNDLNKNRLDYFFSVVGVIAAL 541
           ++  ++ +  LSLASYLSS +  IV  V     + WL  +D++K++LD F+  +  ++ L
Sbjct: 488 SIGNSLLYLGLSLASYLSSAMVSIVHSVTARGGQSWL-TDDIDKSKLDCFYYFIAALSTL 539