CSPI04G13870 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G13870
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	Chr4: 11788813 .. 11793591 (-)
RNA-Seq Expression	CSPI04G13870
Synteny	CSPI04G13870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTAAATTGGATAATATGTTTTTAGCTTATCGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGCGAATCCCGCCGTTTGGTGCTCTGTTCCGATCTCCCATTTCACCTCTCTTCCCCTTACCCCTTCAATTCTCAGACCTCTTCTTCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCATCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTGTGTTATGTTATATGGTTCTTGTGTTTTCTTGCAAATTATGGTAGCTGATAGAGTGAATGCTGGCGCAATTGTGAGTAATCAAATGGACATTTCAGCTCGAAGGTAATAACCTTTTTGTGTGAACGCACTTTAATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCCTCTAAGTCCATCTAGAGTTACAAAGGTTAATTTTTCCAACGAATATTCAAATGAAATAAAGGATTTGTTGGGAAAGTTGAATATAATTCAAGATACCCACCAGTAATGTCTTTCTTCTGTAGTTTGTGCTCAAGAGAGATCTTTGAGCTGAAAATGTTATGTAAATTGGTTTTCAGGTTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTAAGTGCATTTACAACTACAATTTTATCCTATAGCTTTGTTGGATATGTTAACTATATATTAATACATCCAATCAATTTACTTGTAGCTGTTCCGGCTGACCATCTTCCTCATTCATTCTTTTCTAGCATGACAAACTGGTATTGGGTTCTCATATATGTCTACTGAATATTGTTTATTTCATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGCAACACAAGAATAAAGTTTGGTATGTTCATTAGATTGACATGAAAATTTGGTTATATTGTCAAGCTTTCTATTTATGTTTATGATTTCGTTTGTATTAATCTGAAATATGGCAAGCCCTGCTTTTTCCAGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTATCTGGAAACGATTGCATGGACCTTTCTTTTAATCTAACCTGTCCTATAGGGAGTATCGGTTATTCCTTGCTTAACTTCAATATTTTGTGGTAGGGAAAGGGATCGTAGTGAGGTTTGGTCTTTCATTAGATTTCATGTGTCTATTTGGGCTTCGATTTCAAAGTCCTTTTGTAATTATTCCATAGGTAATATCCTTCTAAGTTGGTCCCCCTTCCTTGAGTTGGGGTTGCTCTCTGTGGGCTGGTTTTTTTTGTATGCCCTTTAGGTAGGACACTATTTTGCTATTTTCATGACTTTTGTCTATATTCTACACTACTCATGGCAGTTAGTAATGCCTTTAATAATTTAACTTGAAGTTTCATGTATAAACTCAGTGGAATAGATATGACTAATATTGCATTATACGGCCAATATTTGTTGCAATCCTTCAGGAAACAACATCATTAATACGCTTTATGATTTATGTATCACTTACCTAGTTCAAGGTTTATCATCAAACCCATTTATCCCTTCCTTGTTCCTTCTTGCTCCAAAATAAAGATTTCTTATTTTACTTTGAATATTGTTGTTCTTAGGTCAAGGATCAAACGTATTTTTTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATCCCAAAGGTTTGTTCAAGCTAAATTTGTAGTATTTGTTATCATGTATTTTTTTTTAGGTATCTCTTACGTTTCAGACTAGTGTAAATTTGTATGATTTGGTACTGGTCTTCTAAGCTTGTACAGACATAAACACCTAGTGGCTAACATTGGTTGCATTCAGTTGAGAAATTTGTGTATTTAATAAAAGTTAAAAATTGTATTAGAGACTTTGGCACGCCAGTTGATGTAGATAACATTTTAGTGATTTGTGGATGTTATATTGCAGTATTGCAGTTTGAACCTATTAAGTTTTTACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTAATATCATGCTAACTCCTTTCCCAACTAGCTACATTCTTTCAACTTATGCTCTATCTTTTCTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATGTTGTTAATATGTTTTCTAGGGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTACTTTGGATGTGGTGATTCATACATTTCTTTTGTTTGTCTTTTTTTTTCGGGAGGCTAATGCCTTTCTCAAAATTCTTTGTTTCAATACTTATATATTTCTAATATTTTGGTGTTGCTATTTTATATTTTAGAATATTCTTTACTACTTTTTAGAACTATTTATAGTAATAAAGGCTGGCCTAAAGGATTTAGTACAAGATTTGCCAACACAAGTTGAAATTTTCTGATGGCTATACTTTCACTGAGCAAGTGTTTTATTTCCTTATTCTTCCTGACAGCAACTATTTTCATTTATCCTGTTTCTGAAGATGGGTCCTGTTTAAGTTATCTTGTGGTAGAATTCATGTAGAACCTGTTTCTTTGACGTGTAGATAAAATTTAGTGAATTTGTAGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACAATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTTTCAGTTTCTTTTCTTTAAGAACAAATTTGATGATCTCTAGCTGTTGTATGACCATTTTACCAAAACTTCAAGAACATCTTCAACCGAGAGATTTGAGCATTCCGTTGTGCCAGTTTAGGATTTAGTGACGTGATTACATTGAGATTATTTCCAATCTGGTGCTTCGCAAGCTCACATTCTTTACTTACGTCTCTTGCTGAGCTATATTACAATGACGATTTCAGCTTTTTTGCCTTCATCTAATGCAGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTGTTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGAGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTTCCTCCAGACCAATTTACTGAGCTTCAATGTAAGGTATAGTTTCAGCTTCCATCTCCTCCCATATTTCTTGTTAGTTTCAGTACTTCAAGTTGGCCTGGCCGTAACAGTTTAACATAAAAGAAAATTTTCTTAACTTTTTTACAAACCTTTCCAGAGAACCCATCAGTTTATTCCTGCAAGCTTGTGTTGGAAGTTTGACAAGCATGCTTGGTAATATTGATAATCCGGAAATGTTGACTGTTACATGAAAAAAAGAAAAATTGTCTTCCATGATTATTTAATAGAACAAGCATCTCGACATAATCTGTTTTTCTTTCAAACAAAGGATTTAAGTTTAGAATTCAATTCTATCTGTTAGGGGACTGTGGCCTTTCTCTTTGAGAAGGGAAAATGTAGTAAGATTAAGTAGATGATCTAGAAATGTTATGGGGAGGCTATGTCCGTTATATTCCGCCCATTTGCTTTGAGATTGTGTTATTGGACTCTAGTTCTCATCATGTCATTTCGACTTCATTGTTTCATAATTTTATACAATTCAATTTCTAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAGTGAGGATGGACATGAAGACGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTATGAAAGAATCTAAAGCCAAAGACGATACAGAAATACACGACAAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACACCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTTTCTTTTAAAATTAGAGTGCTATATTGAAATCATGATTCATGGAATGGTAATGAGATTTTATTGACGGATGTAACGTGTAAACAACAAATGAGGTTTGCTTTTTAGATTACCGTTGTATCAACTCTATACGTTTTTTAAATGACACAATTTAGCGTTTGATGTATTATGATAAGCTTTGTTGGCTTCCCCTGC

mRNA sequence

ATGAAATTAAATTGGATAATATGTTTTTAGCTTATCGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGCGAATCCCGCCGTTTGGTGCTCTGTTCCGATCTCCCATTTCACCTCTCTTCCCCTTACCCCTTCAATTCTCAGACCTCTTCTTCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCATCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGCAACACAAGAATAAAGTTTGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTCAAGGATCAAACGTATTTTTTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATCCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTAGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACAATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTGTTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGAGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTTCCTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAGTGAGGATGGACATGAAGACGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTATGAAAGAATCTAAAGCCAAAGACGATACAGAAATACACGACAAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACACCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTTTCTTTTAAAATTAGAGTGCTATATTGAAATCATGATTCATGGAATGGTAATGAGATTTTATTGACGGATGTAACGTGTAAACAACAAATGAGGTTTGCTTTTTAGATTACCGTTGTATCAACTCTATACGTTTTTTAAATGACACAATTTAGCGTTTGATGTATTATGATAAGCTTTGTTGGCTTCCCCTGC

Coding sequence (CDS)

ATGATGCTGAGACTGAGAGCGAATCCCGCCGTTTGGTGCTCTGTTCCGATCTCCCATTTCACCTCTCTTCCCCTTACCCCTTCAATTCTCAGACCTCTTCTTCGCCGCCACAACCCTCGTCTCTTCCTCACCCGATTCTCCGCTTCCTCTGCCGTGCATAATACCTCCCTATCTTCTTCATCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCTCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTTCTTTGCAACACAAGAATAAAGTTTGGAGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGACATGGTCAAGGATCAAACGTATTTTTTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTTGGTTGTATCCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTAGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACAATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTGTTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGAGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTTTTCCTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACAGTGAGGATGGACATGAAGACGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGACCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGGTATGAAAGAATCTAAAGCCAAAGACGATACAGAAATACACGACAAGTTAAGGAATGAAGAAGCCACTGTGGTTGAGCAAATAAGAGAAGTATCTCCAGATGAAGCAATGGCCACACCCTCAATGAAATGGCTTGGAAGTCTTAAGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF*

Homology

[Close]

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.5e-100
Identity = 170/365 (46.58%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 382 FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.2e-99
Identity = 167/365 (45.75%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 382 FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  +     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.2e-99
Identity = 167/365 (45.75%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 382 FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  +     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.0e-99
Identity = 166/365 (45.48%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 381
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 382 FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
           F   + K+RHG   YSC L +  +     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 442 GVILE 446
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 352.8 bits (904), Expect = 6.7e-96
Identity = 166/366 (45.36%), Postives = 245/366 (66.94%), Query Frame = 0


Query: 82  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 141
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 142 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 201
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 202 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 262 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 322 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 381
           F+TIGQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 382 FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441
             +L  K+RHG     C + M  +     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 442 GVILES 447
             IL++
Sbjct: 361 AQILQT 352

[Close]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 537/538 (99.81%), Postives = 537/538 (99.81%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60
           MMLRLR NPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60

Query: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420
           RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420

Query: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480

Query: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF
Sbjct: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.7e-304
Identity = 514/539 (95.36%), Postives = 524/539 (97.22%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLR NPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPDQFT LQCKVRHGP FYSCNLTMEH+EDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           P+SGM ESKAKDDT+I D+ RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 5.0e-304
Identity = 513/539 (95.18%), Postives = 524/539 (97.22%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLR NPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 5.0e-304
Identity = 513/539 (95.18%), Postives = 524/539 (97.22%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLR NPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.2e-284
Identity = 481/537 (89.57%), Postives = 503/537 (93.67%), Query Frame = 0


Query: 2   MLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61
           MLRLR NP VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S SSS+
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421
           RR+FRVGSLKWLSG  PDQFTELQCKVRHGPSFYSCNLTME +ED  EDVAVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           +  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of CSPI04G13870 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 537/538 (99.81%), Postives = 537/538 (99.81%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60
           MMLRLR NPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60

Query: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420
           RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDD 420

Query: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 480

Query: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF
Sbjct: 481 ESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of CSPI04G13870 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 514/539 (95.36%), Postives = 524/539 (97.22%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLR NPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPDQFT LQCKVRHGP FYSCNLTMEH+EDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           P+SGM ESKAKDDT+I D+ RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of CSPI04G13870 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.0e-303
Identity = 513/539 (95.18%), Postives = 524/539 (97.22%), Query Frame = 0


Query: 1   MMLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60
           MMLRLR NPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of CSPI04G13870 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 1012.3 bits (2616), Expect = 1.6e-291
Identity = 490/537 (91.25%), Postives = 511/537 (95.16%), Query Frame = 0


Query: 2   MLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61
           MLRLR  P VWCS+PISHFTSLPL+PSILRP+LRR NPRLFLTRFSASSA+  T  SSS 
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360

Query: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421
           RRVFRVGSLKWL+G PPDQF+ LQCKVRHGPSFYSCNLTMEH+EDG ED+AVVHLSEDDQ
Sbjct: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           S +KES+AKDDT+IH +  N+E TV++QI EVS DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

[Close]

BLAST of CSPI04G13870 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 988.4 bits (2554), Expect = 2.4e-284
Identity = 481/537 (89.57%), Postives = 503/537 (93.67%), Query Frame = 0


Query: 2   MLRLRANPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61
           MLRLR NP VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S SSS+
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121
           K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421
           RR+FRVGSLKWLSG  PDQFTELQCKVRHGPSFYSCNLTME +ED  EDVAVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 539
           +  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of CSPI04G13870 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 711.4 bits (1835), Expect = 5.3e-205
Identity = 349/459 (76.03%), Postives = 386/459 (84.10%), Query Frame = 0


Query: 40  RLFLTRFSASSAVH---------NTSLSSSSKPLSDADFL--PFLSCSMPTYPLKVAVLL 99
           R+F + FS+SS+            T L SS++  S   F+   +LSCSMP  PL+VAVLL
Sbjct: 29  RVFPSFFSSSSSSQLPSPQSSNPITPLISSNRNYSRHAFIVDQYLSCSMPEKPLRVAVLL 88

Query: 100 SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPL 159
           SGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++CPW+DDLKYAK VC+QVDVPL
Sbjct: 89  SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWEDDLKYAKHVCEQVDVPL 148

Query: 160 EVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYA 219
           EVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+ME+DYV SGHYA
Sbjct: 149 EVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISDMEYDYVGSGHYA 208

Query: 220 NVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKF 279
            V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ QLKRLLFPLGC+ KDEVRKLA +F
Sbjct: 209 KVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLLFPLGCVKKDEVRKLATQF 268

Query: 280 NLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQ 339
           +LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAESGDFLG HRGFWFYTIGQRQ
Sbjct: 269 DLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAESGDFLGNHRGFWFYTIGQRQ 328

Query: 340 GLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQFTELQCKV 399
           GLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR+FRVGSL+W SG P  +  EL+CKV
Sbjct: 329 GLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWNSGKPSGKVRELRCKV 388

Query: 400 RHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWD 459
           RHGP FYSC+  ME    G  DVAVVHL EDDQGLAAGQFAAFY+G +CIGSGVILESWD
Sbjct: 389 RHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWD 448

Query: 460 DQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKES 488
           DQ FPVC KA++ A +EDK+KLGKP+KI   P     E+
Sbjct: 449 DQCFPVCAKALQLAALEDKTKLGKPVKIMTMPTMSSVEA 483

The following BLAST results are available for this feature:

BLAST of CSPI04G13870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0SMH1	3.5e-100	46.58	tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN...	[more]
O51625	2.2e-99	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ...	[more]
B7J2N7	2.2e-99	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598...	[more]
Q660I8	5.0e-99	45.48	tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496...	[more]
B2S125	6.7e-96	45.36	tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314...	[more]

BLAST of CSPI04G13870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX16	0.0e+00	99.81	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7TDN6	1.7e-304	95.36	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3B8G8	5.0e-304	95.18	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BNL8	5.0e-304	95.18	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1FY63	1.2e-284	89.57	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of CSPI04G13870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142116.1	0.0e+00	99.81	uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy...	[more]
KAA0041410.1	3.6e-304	95.36	tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa]	[more]
XP_008449720.1	1.0e-303	95.18	PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp...	[more]
XP_038901654.1	1.6e-291	91.25	tRNA-specific 2-thiouridylase MnmA [Benincasa hispida]	[more]
XP_022944568.1	2.4e-284	89.57	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]

BLAST of CSPI04G13870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	5.3e-205	76.03	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 74..295 e-value: 2.4E-85 score: 287.4
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 362..451 e-value: 2.7E-14 score: 54.5
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 83..443 e-value: 1.2E-126 score: 422.3
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 71..476
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 83..328
IPR004506	tRNA-specific 2-thiouridylase	TIGRFAM	TIGR00420	TIGR00420	coord: 83..443 e-value: 9.9E-110 score: 364.6
IPR004506	tRNA-specific 2-thiouridylase	HAMAP	MF_00144	tRNA_thiouridyl_MnmA	coord: 82..444 score: 33.298386
IPR004506	tRNA-specific 2-thiouridylase	CDD	cd01998	tRNA_Me_trans	coord: 83..443 e-value: 1.25002E-161 score: 461.606
IPR023382	Adenine nucleotide alpha hydrolase-like domains	GENE3D	2.30.30.280		coord: 296..355 e-value: 7.0E-19 score: 69.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G13870.1	CSPI04G13870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061708	tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0016740	transferase activity