CSPI04G13830 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G13830
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin ligase BIG BROTHER-like
Location	Chr4: 11757815 .. 11762054 (+)
RNA-Seq Expression	CSPI04G13830
Synteny	CSPI04G13830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAACGGTTATCCTTATAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCGGGTGCCTCACATTCTCAGGTACTATAAATCTTTTTTATTTTAGATGCTCTCCATGAATGTCTTGTCTGGAAGCTGGACTTATAGAACTTGTGGCATTTAAATGCTGCTTTATCTTTACCTTATAGACTTGATTCTCTGTTCTGTAGATTTTTTGACAATTGTCTCAAGTAAAAAAGGGGAAATCATGTGACATTTCATCTTCTAACTACTTTTGGCTTCTCAACTATGGCCTTTTCATTTTGCTTTAACTTCCTGATGCATGCTTGATTTGACATTTATTCTCAAGATCAAAGGGTCTTTATATCTGATTTTCATGATTCCAAATTAATAAACTTATTTTCCCGACATGGAAGTATAATATTTTAATTTCATTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACAAGTTTGGGCATTCTGATTTATGGAGCGCATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCACTATTGATGAACACAGGAGGCCGCTCTCGACAGTCCAGAATGAACAGAGTTCAGGAAATAGCGTGTGGGAAGAAAATGCCAATCCTAATATGTCCGGTAACAGCATGGAATGTAAGGGTTAATGCCTGTTTTGTTTCAAGCTTTTGAATGAACACTATAGTATGATTGTTTGTTAAACAGAAGTTTTCAAATAGTATGATTGTTTGTTAAACAGAAGTTGTCAAGTTAAGGTAATGGAACAAACATTCAGATATTGAATGTTACGGTTCTATAAAATCACACGCTGCAAAGAAGAGATACTTATTGACTCTTTATTTAGATTATTTTTCTCAGTTCATGAATGGAGGCATTTGTAAGAGAAAGCAGTTGATGTCATCCATTTCTGGTCAATCGACAAACTTCATTTTTAGGCTGTTTATTATTTCTCTCAGTTCATAAATGATAGTGCAATATGGTTCTGAGGGTTTTCTTATGCTACATGTAGAAAAATTGGCTTGTGCCATATTAGTCATGTTGTAAGATGGGTAACTTATTCAATGCACGAAATCAAATTTAGGGTGACTTAACGAGTATCTTGTAGATGAGATACAATCTTTCTCAGTTCATAAACGATAATGTAAAATATGTTCATTTGTTTTCTTATGCTACACATAGGAAAATGGCTTTAGACGTAGTAGTCATGCTGTAAAGTGGGAAGCTTATTTGATGCAACAATTGAATTTGGGGTGATTTAATGAGTATCCTGTAAATGAGATACAATCGCACCATCCATTTATACACATATCTTCTATCATATTTCAACGTTAACTTGTAATTACTTGGTGCATCTCATTTTACCAAGTACTGTTCTGTTGTAGTATTAACTATCCTGCTAGGTATAGTACCAAAGATATGCACAAAATCTAACAGCAAAATCATAATACAGAAAAATCCTATTTTATATCATTTTATTACTCATGATTGTTTGCTCCTTTTCTTCTTTCTGATTCAATTATAATGTTTATGGAAATTTAAATACCAGTTCCTAAACTCCGTTTTTCTTTATTACTTTTTCAGGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGGTCTGCAGCTTATCGATTCATAAACATGTTATGCCACTAAGTTTCAGTTGTAGTGTTAAGGTTGTTTTCGGTGGAGTACATTTTTGGTTAGTGTCCTGTATTCTGTGGTTCATGCTGGCAACCAGTCACATACTTGTGTTAATACATAAAATAAAATTATTCCTGTAAGATATAAAACTGGCTTATCTGAAATCACAGAATAAAGGAAAAGATAGAGGATATTAGAATACAAATTCATTTCTAATCCAGTGGTGACAAACTTGGTGGCGGTTCAACTCCCTTAACATCTATTATTCCAGATTTTCTATTCCGCGCCAAATAGACCTGGAATTTACAGTTCCAATGCTTCAGAAGACAACTATAAATGCATGATTCCTAATCTGTTCCATCTTGAAATGTGCAGACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGTTAGAGCAGCTGATTTTCTTTCCTATTTACTTTTGTATATTACTGAACAAAATGTTTTCTCCTGTAACTTTATATGTTCTTATTGAAGCTATATTTACTTCAATGGCTATTTATTATGATGGATATTGCTAATTGAAATGTTATATTACGGCCAGTCAGTAAGAAGTCTGCTGTGTAGCAAAACCAGTAGCCATTACAGCTAATAGGCTAGGTGTTGGACGCTTTCTTGCTAAGGTAGAAAGGATAGAAATGATGGCAGAAGTTAACCTAATTGTTTATTCTGCGTTTTGGTTGTTGGTACACTTCGACGTAATTTTCTATCCTTGAACTTGGTTTCTTGGAGGGCCTTACATATATACCAAAGAAAGCTTGCTTCTTTGGAGCGCTCTGCCTGTTGACGCTGCTTTTTAAGAAGCTAAAATTGATTAGATAGGAGCATAGCTGCATAAGTAATCAGCTGTAAATCCACTCAATACATTCTGAAATCAAGTTCCATGTTTAATAAACGTAAAATTTGTCTGGCTAACTGTTATATGTAAAGTCAACTATATGCCTCCTGAATTATGTCGATTACTCTCTTCAATAAAGTGACGGTTTAAAGAAACTTATTTCAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTAACACTAGATTGATTGATCATCTTATCTCGAAATATTTTAAACTTTGTATTATTTTTCGGTTGTTTTTTAGAATTGTTCCAGCTATCAAGATGTAGTAATTCTCACCAATGCCCTTATACTTAGTAACGCCTATAACAAGTGGGGTATCGGGGGGTTTGATAAGCAACACATAATAATTATTGCCAAGCTATGTTTATTTTGACTTGTGTACCTCATATTTTCATTTTCATTATTATTGTTAAGAACAATATACAGTTACGATGAATGATCCTTCTCAATATTATCTCATTTACTCAAAATCATCTCAATAAAGAGAGAAAACAAGTGGTTTGGCCGACTAGCCTGTCTGCTACTTGCAACGCCACCCTTTATTCAATGGGGAAGAAGGAAAACGATCAATAGATTCAAATTGGTGCACAAGCCTTCCTCATTAACTTTTTTGAGGAATTACCGATTGACAATGATATATTTCTTGCATTAATTTTTTTAACATTATATATATGAAGACACATCTTGATTTGTATACAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGTGACTTTCTGATATCTTTTATCCCTCTTTTAGTAAAACGTACAATCAGCAATTTTACTCAATATTGCATAATCTCTCTCCTCCAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAAAGAAAAGAAGGAAAAGAAAAAAAAGGCTTTGATTAATTGTCATGAGAAAGGGAACCCTTAAAAAGGTTTGGAGTATACTTATTTCGTTTCCTTTCTCATTTACAGCTTTACACAATCGTCGTTTGATGTTAAAGATTAGGAGAAATCTTTGATTTCAATACAGAGGATGATAGAGCCAGATAGTAGAATTTGATTGAAATCAATTTGTTTGTTTGTTTGTTTGGTTTTGTTTTTGTTATTTTTTCAACTTGGCTCATATTGAACCACGACGACTTGGTTTTTGGCTCTACCGATTATAAATATTTCTTATTAGTTGGAGCCTGGAGGGGGCAGAATTTGATGGACAAAAATGGTCAAACTCAAAACTCAAATCCAGTTCATTTTTTTGCCTCAACGTGCTTCCTAGTTGAAGCTATGGGGGAAATAGTCTTCTTCTTCTTCTTCTTCTTCTTTTTTCTTCTTTGTTTTTTTTTTTTTTCCATTTTATATAAGCAAGTTGAGTTATACACCATAGCTTTTAATTATATAGAAAATCACCATCTCACACCTTGGAATTATCTTCTAAGAAAATCG

mRNA sequence

AAAATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAACGGTTATCCTTATAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCGGGTGCCTCACATTCTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACAAGTTTGGGCATTCTGATTTATGGAGCGCATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCACTATTGATGAACACAGGAGGCCGCTCTCGACAGTCCAGAATGAACAGAGTTCAGGAAATAGCGTGTGGGAAGAAAATGCCAATCCTAATATGTCCGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGGTCTGCAGCTTATCGATTCATAAACATGTTATGCCACTAAGTTTCAGTTGTAGTGTTAAGGTTGTTTTCGGTGGAACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAAAGAAAAGAAGGAAAAGAAAAAAAAGGCTTTGATTAATTGTCATGAGAAAGGGAACCCTTAAAAAGGTTTGGAGTATACTTATTTCGTTTCCTTTCTCATTTACAGCTTTACACAATCGTCGTTTGATGTTAAAGATTAGGAGAAATCTTTGATTTCAATACAGAGGATGATAGAGCCAGATAGTAGAATTTGATTGAAATCAATTTGTTTGTTTGTTTGTTTGGTTTTGTTTTTGTTATTTTTTCAACTTGGCTCATATTGAACCACGACGACTTGGTTTTTGGCTCTACCGATTATAAATATTTCTTATTAGTTGGAGCCTGGAGGGGGCAGAATTTGATGGACAAAAATGGTCAAACTCAAAACTCAAATCCAGTTCATTTTTTTGCCTCAACGTGCTTCCTAGTTGAAGCTATGGGGGAAATAGTCTTCTTCTTCTTCTTCTTCTTCTTTTTTCTTCTTTGTTTTTTTTTTTTTTCCATTTTATATAAGCAAGTTGAGTTATACACCATAGCTTTTAATTATATAGAAAATCACCATCTCACACCTTGGAATTATCTTCTAAGAAAATCG

Coding sequence (CDS)

ATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAACGGTTATCCTTATAGTACTGCTTCTTTTATGGAATACTTTGAAGGTCTTACGTATGATCATGTGAATTTCATTTTTTCGGGTGCCTCACATTCTCAGGAGACTGTTTGTCCATCAACTAACTCAAGTTACAAGTTTGGGCATTCTGATTTATGGAGCGCATCATACTTTGATGCTCAATCGTTTGAGGTTCAAGATCATGAATCCACTATTGATGAACACAGGAGGCCGCTCTCGACAGTCCAGAATGAACAGAGTTCAGGAAATAGCGTGTGGGAAGAAAATGCCAATCCTAATATGTCCGGTAACAGCATGGAATGCCCTCGGAGGCATTCAAATTATCATGAGTATCAGGTCTGCAGCTTATCGATTCATAAACATGTTATGCCACTAAGTTTCAGTTGTAGTGTTAAGGTTGTTTTCGGTGGAACTATTTGGCAAGAGATTGTTGATCCTGATAACATGACTTATGAGGAATTAGTAGATTTAGGTGAGACAGTAGGAACTCAAAGTAGAGGCCTTACACAAGAACTAATTGCATTGCTTCCAGTATCGAGGTACAGATGGGGATTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTGATATGCCAAATGGAATACAAACGTGGGGATAAAATAATTACTCTACCATGCAAACACAAGTACCATACAAGTTGCGGGACCAAGTGGCTTAGTATCAAGAAGGCATGTCCCATTTGCTACACTGAGGTTTTTGGTGACACTTCAAAACGATAA

Protein sequence

MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFGHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMECPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTSKR*

Homology

[Close]

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 8.9e-41
Identity = 95/265 (35.85%), Postives = 144/265 (54.34%), Query Frame = 0


Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN-SSYKFGHSDLW 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N ++YKFG S   
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 68  SASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMECPRRH- 127
           +AS++   S+++ DH S                S G + W+ +   N++ +      R  
Sbjct: 70  NASFYG--SYDMNDHLS--------------RMSIGRTNWDYHPMVNVADDPENTVARSV 129

Query: 128 -----SNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 187
                  + E + C  + H    P               WQ+ +DPD MTYEELV+LGE 
Sbjct: 130 QIGDTDEHSEAEECIANEHDPDSP------------QVSWQDDIDPDTMTYEELVELGEA 189

Query: 188 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 247
           VGT+SRGL+QELI  LP  +Y++G    +K   ERCVICQ++YK G++ + LPCKH YH+
Sbjct: 190 VGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHS 246

Query: 248 SCGTKWLSIKKACPICYTEVFGDTS 265
            C +KWLSI K CP+C +EVFG+ S
Sbjct: 250 ECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.8e-25
Identity = 52/106 (49.06%), Postives = 70/106 (66.04%), Query Frame = 0


Query: 159 WQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVIC 218
           W E +DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   NE CVIC
Sbjct: 235 WDE-MDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTNESCVIC 294

Query: 219 QMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTS 265
           +++Y+  + +I LPCKH YH+ C   WL I K CP+C  EV   TS
Sbjct: 295 RLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 9.0e-17
Identity = 40/95 (42.11%), Postives = 56/95 (58.95%), Query Frame = 0


Query: 163 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 222
           +D DNM+YEEL+ LGE +G  S GL++E+I  +          +      E C +CQ EY
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 223 KRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYT 258
             GD + TL C H++HT+C  +WL +K  CPIC T
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.3e-15
Identity = 43/100 (43.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 163 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKK----SRNERCVIC 222
           +D DNM+YEEL+ LGE +G  S GL++E+I L  + +++    S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 223 QMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTE 259
           Q EY  GD + TL C H++H  C  +W+ IK  CPIC TE
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 8.4e-15
Identity = 39/93 (41.94%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 163 VDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEY 222
           +D DNM+YEEL+ L E +G  S GL++E +  L   R    +        E C ICQ EY
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEY 627

Query: 223 KRGDKIITLPCKHKYHTSCGTKWLSIKKACPIC 256
             GD + TL C H +H  C  +WL +K  CPIC
Sbjct: 628 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL
Match: A0A0A0L1Z5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.4e-137
Identity = 241/266 (90.60%), Postives = 241/266 (90.60%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG
Sbjct: 9   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 68

Query: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120
           HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC
Sbjct: 69  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 128

Query: 121 PRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGETV 180
           PRRHSNYHEYQ                         TIWQEIVDPDNMTYEELVDLGETV
Sbjct: 129 PRRHSNYHEYQ-------------------------TIWQEIVDPDNMTYEELVDLGETV 188

Query: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240
           GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS
Sbjct: 189 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 248

Query: 241 CGTKWLSIKKACPICYTEVFGDTSKR 267
           CGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 249 CGTKWLSIKKACPICYTEVFGDTSKR 249

[Close]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL
Match: A0A1S3BNC2 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491520 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.3e-130
Identity = 231/267 (86.52%), Postives = 235/267 (88.01%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSME 120
           G+SDLWS SYFDAQ FEVQDHES I EHRRPLSTVQNEQS GNSVWEENANPNMSGNSME
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVWEENANPNMSGNSME 120

Query: 121 CPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 180
           CPRRHSNYHEYQ                         TIWQ+IVDPDNMTYEELVDLGET
Sbjct: 121 CPRRHSNYHEYQ-------------------------TIWQDIVDPDNMTYEELVDLGET 180

Query: 181 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 240
           VGTQSRGLTQELIALLPVS YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHT
Sbjct: 181 VGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHT 240

Query: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 267
           SCGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 242

[Close]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL
Match: A0A1S3BNM5 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491520 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 7.9e-125
Identity = 226/267 (84.64%), Postives = 230/267 (86.14%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSME 120
           G+SDLWS SYFDAQ FEVQDHES I EHRRPLSTVQNEQS GNSVWEENANPNMSG    
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVWEENANPNMSG---- 120

Query: 121 CPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 180
            PRRHSNYHEYQ                         TIWQ+IVDPDNMTYEELVDLGET
Sbjct: 121 -PRRHSNYHEYQ-------------------------TIWQDIVDPDNMTYEELVDLGET 180

Query: 181 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 240
           VGTQSRGLTQELIALLPVS YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHT
Sbjct: 181 VGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHT 237

Query: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 267
           SCGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 237

[Close]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL
Match: A0A6J1K714 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.7e-109
Identity = 200/269 (74.35%), Postives = 217/269 (80.67%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFS  SHS+ETVCP+TNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSAPSHSEETVCPATNSSYYKF 60

Query: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHR--RPLSTVQNEQSSGNSVWEENANPNMSGNS 120
            H+D WSAS FD QSFEVQ HES IDEHR  +  STV +EQS GN V EEN NP+M+ NS
Sbjct: 61  EHADSWSASCFDTQSFEVQGHESVIDEHRMQQEFSTVPDEQSLGNRVREENVNPDMASNS 120

Query: 121 MECPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLG 180
           MECPRRHSNYHEYQ                         TIWQ+I+DPDNMTYEELVDLG
Sbjct: 121 MECPRRHSNYHEYQ-------------------------TIWQDIIDPDNMTYEELVDLG 180

Query: 181 ETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKY 240
           ETVG+QSRGL+QELIALLPVS+++  FFSRKKSR ERCVICQM YK GD+ ITLPCKHKY
Sbjct: 181 ETVGSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKY 240

Query: 241 HTSCGTKWLSIKKACPICYTEVFGDTSKR 267
           HTSCG+KWLSI KACPICYTEV+GD SKR
Sbjct: 241 HTSCGSKWLSINKACPICYTEVYGDISKR 244

[Close]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL
Match: A0A6J1D445 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.0e-108
Identity = 203/272 (74.63%), Postives = 219/272 (80.51%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSGASHSQETV PSTNSS YK
Sbjct: 13  MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETVYPSTNSSYYK 72

Query: 61  FGHSDLWSASYFDAQSFEVQDHESTIDEHRRP--LSTVQNEQSSGNSVWEENANPNMSG- 120
           FGHSD WS+SYFDAQS EV  HEST+DEHRRP   ST+ NEQSSGN  WEENANPNMS  
Sbjct: 73  FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTLPNEQSSGNRAWEENANPNMSSN 132

Query: 121 -NSMECPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELV 180
            N+MECPRRH NY EYQ                         TIWQ+IVDPDNMTYEEL+
Sbjct: 133 TNTMECPRRHPNYQEYQ-------------------------TIWQDIVDPDNMTYEELL 192

Query: 181 DLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCK 240
           DLGETVGT +RGL QELIALLPVS+Y+ GFF  KKSRNERCVICQMEYKRGD+ ITLPCK
Sbjct: 193 DLGETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCK 252

Query: 241 HKYHTSCGTKWLSIKKACPICYTEVFGDTSKR 267
           H+YHT CGTKWLSI KACPICYTEVFG TSKR
Sbjct: 253 HRYHTCCGTKWLSINKACPICYTEVFGGTSKR 259

[Close]

BLAST of CSPI04G13830 vs. NCBI nr
Match: XP_004142225.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_011653590.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739641.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_031739642.1 E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >KAE8649510.1 hypothetical protein Csa_017925 [Cucumis sativus])

HSP 1 Score: 498.0 bits (1281), Expect = 4.9e-137
Identity = 241/266 (90.60%), Postives = 241/266 (90.60%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60

Query: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120
           HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC
Sbjct: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120

Query: 121 PRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGETV 180
           PRRHSNYHEYQ                         TIWQEIVDPDNMTYEELVDLGETV
Sbjct: 121 PRRHSNYHEYQ-------------------------TIWQEIVDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240
           GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS
Sbjct: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240

Query: 241 CGTKWLSIKKACPICYTEVFGDTSKR 267
           CGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 CGTKWLSIKKACPICYTEVFGDTSKR 241

[Close]

BLAST of CSPI04G13830 vs. NCBI nr
Match: XP_031739643.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X2 [Cucumis sativus])

HSP 1 Score: 491.1 bits (1263), Expect = 6.0e-135
Identity = 240/266 (90.23%), Postives = 240/266 (90.23%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ TVCPSTNSSYKFG
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ-TVCPSTNSSYKFG 60

Query: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120
           HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC
Sbjct: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120

Query: 121 PRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGETV 180
           PRRHSNYHEYQ                         TIWQEIVDPDNMTYEELVDLGETV
Sbjct: 121 PRRHSNYHEYQ-------------------------TIWQEIVDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240
           GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS
Sbjct: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240

Query: 241 CGTKWLSIKKACPICYTEVFGDTSKR 267
           CGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 CGTKWLSIKKACPICYTEVFGDTSKR 240

[Close]

BLAST of CSPI04G13830 vs. NCBI nr
Match: XP_031739644.1 (E3 ubiquitin-protein ligase BIG BROTHER isoform X3 [Cucumis sativus])

HSP 1 Score: 481.1 bits (1237), Expect = 6.2e-132
Identity = 236/266 (88.72%), Postives = 236/266 (88.72%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSSYKFG 60

Query: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMEC 120
           HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSG     
Sbjct: 61  HSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSG----- 120

Query: 121 PRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGETV 180
           PRRHSNYHEYQ                         TIWQEIVDPDNMTYEELVDLGETV
Sbjct: 121 PRRHSNYHEYQ-------------------------TIWQEIVDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 240
           GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS
Sbjct: 181 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 236

Query: 241 CGTKWLSIKKACPICYTEVFGDTSKR 267
           CGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 CGTKWLSIKKACPICYTEVFGDTSKR 236

[Close]

BLAST of CSPI04G13830 vs. NCBI nr
Match: XP_008449725.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo])

HSP 1 Score: 473.4 bits (1217), Expect = 1.3e-129
Identity = 231/267 (86.52%), Postives = 235/267 (88.01%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSME 120
           G+SDLWS SYFDAQ FEVQDHES I EHRRPLSTVQNEQS GNSVWEENANPNMSGNSME
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVWEENANPNMSGNSME 120

Query: 121 CPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 180
           CPRRHSNYHEYQ                         TIWQ+IVDPDNMTYEELVDLGET
Sbjct: 121 CPRRHSNYHEYQ-------------------------TIWQDIVDPDNMTYEELVDLGET 180

Query: 181 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 240
           VGTQSRGLTQELIALLPVS YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHT
Sbjct: 181 VGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHT 240

Query: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 267
           SCGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 242

[Close]

BLAST of CSPI04G13830 vs. NCBI nr
Match: XP_008449726.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo])

HSP 1 Score: 456.4 bits (1173), Expect = 1.6e-124
Identity = 226/267 (84.64%), Postives = 230/267 (86.14%), Query Frame = 0


Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTNSS-YKF 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQ+TVCPSTNSS YKF
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQDTVCPSTNSSYYKF 60

Query: 61  GHSDLWSASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSME 120
           G+SDLWS SYFDAQ FEVQDHES I EHRRPLSTVQNEQS GNSVWEENANPNMSG    
Sbjct: 61  GNSDLWSTSYFDAQLFEVQDHESAIYEHRRPLSTVQNEQSLGNSVWEENANPNMSG---- 120

Query: 121 CPRRHSNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 180
            PRRHSNYHEYQ                         TIWQ+IVDPDNMTYEELVDLGET
Sbjct: 121 -PRRHSNYHEYQ-------------------------TIWQDIVDPDNMTYEELVDLGET 180

Query: 181 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 240
           VGTQSRGLTQELIALLPVS YRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKH+YHT
Sbjct: 181 VGTQSRGLTQELIALLPVSSYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHRYHT 237

Query: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 267
           SCGTKWLSIKKACPICYTEVFGDTSKR
Sbjct: 241 SCGTKWLSIKKACPICYTEVFGDTSKR 237

[Close]

BLAST of CSPI04G13830 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 6.3e-42
Identity = 95/265 (35.85%), Postives = 144/265 (54.34%), Query Frame = 0


Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN-SSYKFGHSDLW 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N ++YKFG S   
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 68  SASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMECPRRH- 127
           +AS++   S+++ DH S                S G + W+ +   N++ +      R  
Sbjct: 70  NASFYG--SYDMNDHLS--------------RMSIGRTNWDYHPMVNVADDPENTVARSV 129

Query: 128 -----SNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 187
                  + E + C  + H    P               WQ+ +DPD MTYEELV+LGE 
Sbjct: 130 QIGDTDEHSEAEECIANEHDPDSP------------QVSWQDDIDPDTMTYEELVELGEA 189

Query: 188 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 247
           VGT+SRGL+QELI  LP  +Y++G    +K   ERCVICQ++YK G++ + LPCKH YH+
Sbjct: 190 VGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHS 246

Query: 248 SCGTKWLSIKKACPICYTEVFGDTS 265
            C +KWLSI K CP+C +EVFG+ S
Sbjct: 250 ECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CSPI04G13830 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 6.3e-42
Identity = 95/265 (35.85%), Postives = 144/265 (54.34%), Query Frame = 0


Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGASHSQETVCPSTN-SSYKFGHSDLW 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q+ +  + N ++YKFG S   
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNAYKFGFSGSD 69

Query: 68  SASYFDAQSFEVQDHESTIDEHRRPLSTVQNEQSSGNSVWEENANPNMSGNSMECPRRH- 127
           +AS++   S+++ DH S                S G + W+ +   N++ +      R  
Sbjct: 70  NASFYG--SYDMNDHLS--------------RMSIGRTNWDYHPMVNVADDPENTVARSV 129

Query: 128 -----SNYHEYQVCSLSIHKHVMPLSFSCSVKVVFGGTIWQEIVDPDNMTYEELVDLGET 187
                  + E + C  + H    P               WQ+ +DPD MTYEELV+LGE 
Sbjct: 130 QIGDTDEHSEAEECIANEHDPDSP------------QVSWQDDIDPDTMTYEELVELGEA 189

Query: 188 VGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHT 247
           VGT+SRGL+QELI  LP  +Y++G    +K   ERCVICQ++YK G++ + LPCKH YH+
Sbjct: 190 VGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHS 246

Query: 248 SCGTKWLSIKKACPICYTEVFGDTS 265
            C +KWLSI K CP+C +EVFG+ S
Sbjct: 250 ECISKWLSINKVCPVCNSEVFGEPS 246

[Close]

BLAST of CSPI04G13830 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-26
Identity = 52/106 (49.06%), Postives = 70/106 (66.04%), Query Frame = 0


Query: 159 WQEIVDPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVIC 218
           W E +DPD ++YEEL+ LG+ VGT+SRGL+ + IA LP  RY+ G    +   NE CVIC
Sbjct: 235 WDE-MDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGTNESCVIC 294

Query: 219 QMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVFGDTS 265
           +++Y+  + +I LPCKH YH+ C   WL I K CP+C  EV   TS
Sbjct: 295 RLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of CSPI04G13830 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.9e-22
Identity = 50/105 (47.62%), Postives = 65/105 (61.90%), Query Frame = 0


Query: 164 DPDNMTYEELVDLGETVGTQSRGLTQELIALLPVSRY-----RWGFFSRKK---SRNERC 223
           DP+NMTYEEL +LG++VG   +GL+QE I+ L   +Y      W     KK   + + +C
Sbjct: 175 DPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQC 234

Query: 224 VICQMEYKRGDKIITLPCKHKYHTSCGTKWLSIKKACPICYTEVF 261
            IC MEY +GDKI TLPCKH YH  C ++WL   K C IC  EV+
Sbjct: 235 SICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of CSPI04G13830 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 3.3e-22
Identity = 46/101 (45.54%), Postives = 67/101 (66.34%), Query Frame = 0


Query: 160 QEIVDPDNMTYEELVDLGETVGTQSRGLTQ-ELIALLPVSRYRWGFFSRKKSRNERCVIC 219
           ++ +DPD ++YEEL+ LG+ +G ++RGLT  E+   L  S Y    FS  K+  +RCV+C
Sbjct: 109 EDEIDPDQLSYEELIALGDFIGVENRGLTPIEISTCLNASTY---VFSHNKNEIDRCVVC 168

Query: 220 QMEYKRGDKIITL-PCKHKYHTSCGTKWLSIKKACPICYTE 259
           QME++  + ++ L PC H YH+ C TKWL  KK CPIC +E
Sbjct: 169 QMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSE 206

The following BLAST results are available for this feature:

BLAST of CSPI04G13830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	8.9e-41	35.85	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	1.8e-25	49.06	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	9.0e-17	42.11	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q9ZQF9	1.3e-15	43.00	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	8.4e-15	41.94	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of CSPI04G13830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1Z5	2.4e-137	90.60	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 P...	[more]
A0A1S3BNC2	6.3e-130	86.52	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3BNM5	7.9e-125	84.64	E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1K714	2.7e-109	74.35	E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765...	[more]
A0A6J1D445	1.0e-108	74.63	E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of CSPI04G13830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142225.1	4.9e-137	90.60	E3 ubiquitin-protein ligase BIG BROTHER isoform X1 [Cucumis sativus] >XP_0116535...	[more]
XP_031739643.1	6.0e-135	90.23	E3 ubiquitin-protein ligase BIG BROTHER isoform X2 [Cucumis sativus]	[more]
XP_031739644.1	6.2e-132	88.72	E3 ubiquitin-protein ligase BIG BROTHER isoform X3 [Cucumis sativus]	[more]
XP_008449725.1	1.3e-129	86.52	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X1 [Cucumis melo]	[more]
XP_008449726.1	1.6e-124	84.64	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like isoform X2 [Cucumis melo]	[more]

BLAST of CSPI04G13830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	6.3e-42	35.85	RING/U-box superfamily protein	[more]
AT3G63530.2	6.3e-42	35.85	RING/U-box superfamily protein	[more]
AT3G19910.1	1.3e-26	49.06	RING/U-box superfamily protein	[more]
AT5G52140.1	1.9e-22	47.62	RING/U-box superfamily protein	[more]
AT3G47180.1	3.3e-22	45.54	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 215..255 e-value: 2.6E-6 score: 37.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 214..255 e-value: 2.7E-11 score: 43.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 215..255 score: 11.087743
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 177..260 e-value: 9.6E-18 score: 65.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 93..119
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..119
None	No IPR available	PANTHER	PTHR46400:SF5	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..265
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 214..260
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G13830.1	CSPI04G13830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity