CSPI04G13510 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G13510
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Lipoxygenase
Location	Chr4: 11540896 .. 11541498 (+)
RNA-Seq Expression	CSPI04G13510
Synteny	CSPI04G13510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGAGAAATACAATACAAGAACCAACAGAAAAAGCAAGAGGGGCCATGGTGACAAAAAAGACGAGCCATGGTGGCCACTTCTCAACACTCCTCAAGATCTAATAGACATAGTGACAAACATTGCTTGGGTAGCATCAGCACACCATGCGGCTGTGAACTTTGGGCAATATTCATATGCAGCGTATTTTCCAAACATGCCAAGTATAGCAAGAACCAATATGCCTACAGAAGATAATAATCCTGCACTTTGGAAAAGCTTCTTAGAAAAGCCGGAGGAGTTGCTTCTAAATTCCTTTCCTTCGCAATATCAAGCAACACAGGTAATGATGGTGTTGAATCTGTTATCAAGTCACTCTCGTGATGAAGAGTTTGTTGGAAATAAAATGGAGCCATCGTGGGGTGATAATCCGACAATAAAGGCGGCATTTGAAAGGTTTAGTGCAAATATAAAAAACTTGGACCTAATAATTGATACCAGGAACGCTGATTACAACTTGAAGAATAGAACTGGAGCTGGAGTTCCACCTTATGAACTTTTGAAGCCATTTTCAGGTCCTGGAGTTACCGGAAAGGGAGTTCCATACAGTGTTTCTATTTAA

mRNA sequence

ATGATAGAGAAATACAATACAAGAACCAACAGAAAAAGCAAGAGGGGCCATGGTGACAAAAAAGACGAGCCATGGTGGCCACTTCTCAACACTCCTCAAGATCTAATAGACATAGTGACAAACATTGCTTGGGTAGCATCAGCACACCATGCGGCTGTGAACTTTGGGCAATATTCATATGCAGCGTATTTTCCAAACATGCCAAGTATAGCAAGAACCAATATGCCTACAGAAGATAATAATCCTGCACTTTGGAAAAGCTTCTTAGAAAAGCCGGAGGAGTTGCTTCTAAATTCCTTTCCTTCGCAATATCAAGCAACACAGGTAATGATGGTGTTGAATCTGTTATCAAGTCACTCTCGTGATGAAGAGTTTGTTGGAAATAAAATGGAGCCATCGTGGGGTGATAATCCGACAATAAAGGCGGCATTTGAAAGGTTTAGTGCAAATATAAAAAACTTGGACCTAATAATTGATACCAGGAACGCTGATTACAACTTGAAGAATAGAACTGGAGCTGGAGTTCCACCTTATGAACTTTTGAAGCCATTTTCAGGTCCTGGAGTTACCGGAAAGGGAGTTCCATACAGTGTTTCTATTTAA

Coding sequence (CDS)

ATGATAGAGAAATACAATACAAGAACCAACAGAAAAAGCAAGAGGGGCCATGGTGACAAAAAAGACGAGCCATGGTGGCCACTTCTCAACACTCCTCAAGATCTAATAGACATAGTGACAAACATTGCTTGGGTAGCATCAGCACACCATGCGGCTGTGAACTTTGGGCAATATTCATATGCAGCGTATTTTCCAAACATGCCAAGTATAGCAAGAACCAATATGCCTACAGAAGATAATAATCCTGCACTTTGGAAAAGCTTCTTAGAAAAGCCGGAGGAGTTGCTTCTAAATTCCTTTCCTTCGCAATATCAAGCAACACAGGTAATGATGGTGTTGAATCTGTTATCAAGTCACTCTCGTGATGAAGAGTTTGTTGGAAATAAAATGGAGCCATCGTGGGGTGATAATCCGACAATAAAGGCGGCATTTGAAAGGTTTAGTGCAAATATAAAAAACTTGGACCTAATAATTGATACCAGGAACGCTGATTACAACTTGAAGAATAGAACTGGAGCTGGAGTTCCACCTTATGAACTTTTGAAGCCATTTTCAGGTCCTGGAGTTACCGGAAAGGGAGTTCCATACAGTGTTTCTATTTAA

Protein sequence

MIEKYNTRTNRKSKRGHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNMPTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWGDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVPYSVSI*

Homology

[Close]

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 2.4e-70
Identity = 122/185 (65.95%), Postives = 143/185 (77.30%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GHGDK+DEPWWP L TP DLI I+T I WV S HHAAVNFGQYSYA YFPN P++AR+ M
Sbjct: 715 GHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKM 774

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED     W+ F+ KPEE LL  FPSQ QAT+VM +L++LS+HS DEE++G K+EP W 
Sbjct: 775 PTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWA 834

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           ++P I AAFE FS  +K L+ IID RN D  L NR GAGV PYELLKP+S PGVTGKGVP
Sbjct: 835 EDPVINAAFEVFSGKLKELEGIIDARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVP 894

Query: 196 YSVSI 201
           YS+SI
Sbjct: 895 YSISI 899

[Close]

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 2.5e-67
Identity = 118/185 (63.78%), Postives = 143/185 (77.30%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DKKDEPWWP L T QDLI +V+ I WV+S HH+AVNFGQY +  YFPN P+IART M
Sbjct: 715 GHADKKDEPWWPQLKTQQDLIGVVSTIMWVSSGHHSAVNFGQYDFGGYFPNRPTIARTKM 774

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           P ED     W++F+EKPE++LLN FP+Q QAT+VM +L++LSSHS DEE++G  ME SW 
Sbjct: 775 PNEDPTFEEWEAFMEKPEDVLLNCFPTQIQATKVMAILDVLSSHSPDEEYIGTSMEASWE 834

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
             P IK+AFE F   +K LD IID+RN D  L+NRTGAG+  Y+LLKPFSG GVTGKGVP
Sbjct: 835 AEPAIKSAFEEFCGRLKKLDDIIDSRNRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVP 894

Query: 196 YSVSI 201
           YS+SI
Sbjct: 895 YSISI 899

[Close]

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 8.2e-63
Identity = 113/185 (61.08%), Postives = 133/185 (71.89%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GHGDKKDEPWWP+L T  DLI +VT IAWV S HHAAVNFGQY Y  YFPN P+  R  M
Sbjct: 712 GHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRM 771

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED      K F E PE++LL ++PSQ QAT VM+ L+LLS+HS DEE++G + E SW 
Sbjct: 772 PTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWA 831

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           + P I AAFERF   ++ L+ +ID RN +  LKNR GAGV  YELLKP S  GVTG GVP
Sbjct: 832 NEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVP 891

Query: 196 YSVSI 201
           YS+SI
Sbjct: 892 YSISI 896

[Close]

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot
Match: Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-60
Identity = 107/186 (57.53%), Postives = 136/186 (73.12%), Query Frame = 0


Query: 15  RGHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTN 74
           +GH DKKDEPWWP+ +T ++L+ I+T I WV S HHAAVNFGQY YA YFPN P++ R N
Sbjct: 711 KGHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRN 770

Query: 75  MPTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSW 134
           +P E+N     K F+ +PEE+LL S PSQ QA +VM  L++LSSHS DEE++G   EP+W
Sbjct: 771 IPVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAW 830

Query: 135 GDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGV 194
              P +KAAFE+FS  +K  +  ID RN +   KNR GAG+ PYELLKPFS PGVTG+G+
Sbjct: 831 LAEPMVKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGAGIVPYELLKPFSEPGVTGRGI 890

Query: 195 PYSVSI 201
           P S+SI
Sbjct: 891 PNSISI 896

[Close]

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot
Match: P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 3.3e-56
Identity = 102/187 (54.55%), Postives = 134/187 (71.66%), Query Frame = 0


Query: 15  RGHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTN 74
           +GHGDKKD PWWP L++P+ L   +T I WVA+AHHAAVNFGQY +  YFPN PSIART 
Sbjct: 738 KGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTV 797

Query: 75  MPTEDN-NPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPS 134
           MP E+  + A  + FL+ P++ L   FPSQ QAT VM VL++LSSHS DEE++G +    
Sbjct: 798 MPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGEQTRP 857

Query: 135 WGDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKG 194
           W  +  ++AA++ F+A +K ++ +ID RN D  LKNR GAG+ PY+L+KPFS  GVTG G
Sbjct: 858 WNSDAAVQAAYDGFAARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVTGMG 917

Query: 195 VPYSVSI 201
           +P S SI
Sbjct: 918 IPNSTSI 924

[Close]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL
Match: A0A5D3DD89 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00030 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.8e-89
Identity = 157/185 (84.86%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK++EPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y+ YFPN PSI RTNM
Sbjct: 753 GHADKQEEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYSGYFPNRPSITRTNM 812

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTEDNNPALWKSFLEKPEELLL+SFPSQ+QATQVM+V+N+LSSHS DEE++G  MEPSWG
Sbjct: 813 PTEDNNPALWKSFLEKPEELLLDSFPSQFQATQVMLVMNMLSSHSPDEEYIGKDMEPSWG 872

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNPTIKAAFERFS +IKN++LIID RNAD NLKNRTGAGV PYELLKPFS  GVTGKGVP
Sbjct: 873 DNPTIKAAFERFSTSIKNMELIIDHRNADSNLKNRTGAGVTPYELLKPFSESGVTGKGVP 932

Query: 196 YSVSI 201
           YS+SI
Sbjct: 933 YSISI 937

[Close]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL
Match: A0A6J1H2B7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.4e-89
Identity = 156/185 (84.32%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+YA YFP+ PSIARTNM
Sbjct: 718 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNM 777

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 778 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 837

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 838 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 897

Query: 196 YSVSI 201
           YS+SI
Sbjct: 898 YSISI 902

[Close]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL
Match: A0A6J1JXK9 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111490700 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.9e-89
Identity = 155/185 (83.78%), Postives = 171/185 (92.43%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y  YFP+ PSIARTNM
Sbjct: 718 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYGGYFPSRPSIARTNM 777

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 778 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 837

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 838 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 897

Query: 196 YSVSI 201
           YS+SI
Sbjct: 898 YSISI 902

[Close]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL
Match: A0A125S6K8 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX13 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.2e-88
Identity = 155/185 (83.78%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+++PWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y+ YFPN PSI RTNM
Sbjct: 722 GHADKQEKPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYSGYFPNRPSITRTNM 781

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTEDNNPALWKSFLEKPEELLL+SFPSQ+QATQVM+V+N+LSSHS DEE++G  MEPSWG
Sbjct: 782 PTEDNNPALWKSFLEKPEELLLDSFPSQFQATQVMLVMNMLSSHSPDEEYIGKDMEPSWG 841

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNPTIKAAFERFS +IKN++LIID RN+D NLKNRTGAGV PYELLKPFS  GVTGKGVP
Sbjct: 842 DNPTIKAAFERFSTSIKNMELIIDHRNSDSNLKNRTGAGVTPYELLKPFSESGVTGKGVP 901

Query: 196 YSVSI 201
           YS+SI
Sbjct: 902 YSISI 906

[Close]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL
Match: A0A1S3BMS1 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491539 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.2e-88
Identity = 155/185 (83.78%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+++PWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y+ YFPN PSI RTNM
Sbjct: 722 GHADKQEKPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYSGYFPNRPSITRTNM 781

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTEDNNPALWKSFLEKPEELLL+SFPSQ+QATQVM+V+N+LSSHS DEE++G  MEPSWG
Sbjct: 782 PTEDNNPALWKSFLEKPEELLLDSFPSQFQATQVMLVMNMLSSHSPDEEYIGKDMEPSWG 841

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNPTIKAAFERFS +IKN++LIID RN+D NLKNRTGAGV PYELLKPFS  GVTGKGVP
Sbjct: 842 DNPTIKAAFERFSTSIKNMELIIDHRNSDSNLKNRTGAGVTPYELLKPFSESGVTGKGVP 901

Query: 196 YSVSI 201
           YS+SI
Sbjct: 902 YSISI 906

[Close]

BLAST of CSPI04G13510 vs. NCBI nr
Match: TYK21641.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 338.6 bits (867), Expect = 3.7e-89
Identity = 157/185 (84.86%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK++EPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y+ YFPN PSI RTNM
Sbjct: 753 GHADKQEEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYSGYFPNRPSITRTNM 812

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTEDNNPALWKSFLEKPEELLL+SFPSQ+QATQVM+V+N+LSSHS DEE++G  MEPSWG
Sbjct: 813 PTEDNNPALWKSFLEKPEELLLDSFPSQFQATQVMLVMNMLSSHSPDEEYIGKDMEPSWG 872

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNPTIKAAFERFS +IKN++LIID RNAD NLKNRTGAGV PYELLKPFS  GVTGKGVP
Sbjct: 873 DNPTIKAAFERFSTSIKNMELIIDHRNADSNLKNRTGAGVTPYELLKPFSESGVTGKGVP 932

Query: 196 YSVSI 201
           YS+SI
Sbjct: 933 YSISI 937

[Close]

BLAST of CSPI04G13510 vs. NCBI nr
Match: KAG6605891.1 (Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 338.2 bits (866), Expect = 4.9e-89
Identity = 156/185 (84.32%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+YA YFP+ PSIARTNM
Sbjct: 717 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNM 776

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 777 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 836

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 837 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 896

Query: 196 YSVSI 201
           YS+SI
Sbjct: 897 YSISI 901

[Close]

BLAST of CSPI04G13510 vs. NCBI nr
Match: XP_022958148.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 338.2 bits (866), Expect = 4.9e-89
Identity = 156/185 (84.32%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+YA YFP+ PSIARTNM
Sbjct: 718 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNM 777

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 778 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 837

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 838 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 897

Query: 196 YSVSI 201
           YS+SI
Sbjct: 898 YSISI 902

[Close]

BLAST of CSPI04G13510 vs. NCBI nr
Match: XP_023532782.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 338.2 bits (866), Expect = 4.9e-89
Identity = 156/185 (84.32%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+YA YFP+ PSIARTNM
Sbjct: 717 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNM 776

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 777 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 836

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 837 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 896

Query: 196 YSVSI 201
           YS+SI
Sbjct: 897 YSISI 901

[Close]

BLAST of CSPI04G13510 vs. NCBI nr
Match: XP_022995022.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 1.4e-88
Identity = 155/185 (83.78%), Postives = 171/185 (92.43%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH DK+DEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQY+Y  YFP+ PSIARTNM
Sbjct: 718 GHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYGGYFPSRPSIARTNM 777

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED NPALWKSFLEKPEE+LLN+FPSQYQATQVM+VL++LSSHS DEE++G  MEPSWG
Sbjct: 778 PTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWG 837

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           DNP IKAAF+RF+  +K L+LIID RNAD+NLKNRTGAGV PYELLKPFSGPGVTGKGVP
Sbjct: 838 DNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVP 897

Query: 196 YSVSI 201
           YS+SI
Sbjct: 898 YSISI 902

[Close]

BLAST of CSPI04G13510 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 241.5 bits (615), Expect = 5.8e-64
Identity = 113/185 (61.08%), Postives = 133/185 (71.89%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GHGDKKDEPWWP+L T  DLI +VT IAWV S HHAAVNFGQY Y  YFPN P+  R  M
Sbjct: 712 GHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRM 771

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPSWG 135
           PTED      K F E PE++LL ++PSQ QAT VM+ L+LLS+HS DEE++G + E SW 
Sbjct: 772 PTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWA 831

Query: 136 DNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGVP 195
           + P I AAFERF   ++ L+ +ID RN +  LKNR GAGV  YELLKP S  GVTG GVP
Sbjct: 832 NEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVP 891

Query: 196 YSVSI 201
           YS+SI
Sbjct: 892 YSISI 896

[Close]

BLAST of CSPI04G13510 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 184.9 bits (468), Expect = 6.4e-47
Identity = 90/188 (47.87%), Postives = 123/188 (65.43%), Query Frame = 0


Query: 15  RGHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTN 74
           +GH DKKDEPWWP LNT QDL  I+TN+ W+AS  HAA+NFGQY +  Y PN P++ R  
Sbjct: 732 KGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKL 791

Query: 75  MPTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFV--GNKMEP 134
           +P E +    ++ F+  P+   L S P+Q QAT+VM V   LS+HS DEE++    +++ 
Sbjct: 792 IPQETDPD--YEMFMRNPQYSFLGSLPTQLQATKVMAVQETLSTHSPDEEYLIELREVQR 851

Query: 135 SWGDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGK 194
            W  +  +   F +FS  +  ++  I+ RN D  LKNRTGAG+PPYELL P S  GVTG+
Sbjct: 852 HWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGR 911

Query: 195 GVPYSVSI 201
           G+P S+SI
Sbjct: 912 GIPNSISI 917

[Close]

BLAST of CSPI04G13510 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 181.0 bits (458), Expect = 9.3e-46
Identity = 90/186 (48.39%), Postives = 121/186 (65.05%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH D +D  WWP L+T +DL+ ++T I W+ASA HAA+NFGQY Y  Y PN P + R  +
Sbjct: 743 GHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLI 802

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPS-W 135
           P  D +   + SF+E P++   +S PS  Q T+ M V++ LS+HS DEE++G + +PS W
Sbjct: 803 P--DESDPEFTSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIW 862

Query: 136 GDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGV 195
             +  I  AF  FSA I  ++  ID RN D + +NR GAGV PYEL+ P S PGVT +GV
Sbjct: 863 TGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGV 922

Query: 196 PYSVSI 201
           P SVSI
Sbjct: 923 PNSVSI 926

[Close]

BLAST of CSPI04G13510 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 175.6 bits (444), Expect = 3.9e-44
Identity = 89/186 (47.85%), Postives = 120/186 (64.52%), Query Frame = 0


Query: 16  GHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIARTNM 75
           GH D +D  WWP L+T  DL+ I+T + W+ASA HAA+NFGQY Y  Y PN P + R  +
Sbjct: 736 GHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLI 795

Query: 76  PTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPS-W 135
           P  D +   + SF+  PE+   +S PS  Q ++ M V++ LS+HS DEE++G + +PS W
Sbjct: 796 P--DESDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIW 855

Query: 136 GDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKGV 195
             +  I  AF  F+A I  ++  I+ RNAD + +NR GAGV PYELL P S PGVT +GV
Sbjct: 856 TGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGV 915

Query: 196 PYSVSI 201
           P SVSI
Sbjct: 916 PNSVSI 919

[Close]

BLAST of CSPI04G13510 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 167.9 bits (424), Expect = 8.2e-42
Identity = 84/187 (44.92%), Postives = 114/187 (60.96%), Query Frame = 0


Query: 14  KRGHGDKKDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYSYAAYFPNMPSIART 73
           + GHGDKK EPWWP + T ++L++  T I WVASA HAAVNFGQY  A Y PN P+I+R 
Sbjct: 675 EEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQ 734

Query: 74  NMPTEDNNPALWKSFLEKPEELLLNSFPSQYQATQVMMVLNLLSSHSRDEEFVGNKMEPS 133
            MP E  N   ++   + P+++ L +  +Q Q    + ++ +LS+HS DE ++G +    
Sbjct: 735 YMPKE--NTPEFEELEKNPDKVFLKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKE 794

Query: 134 WGDNPTIKAAFERFSANIKNLDLIIDTRNADYNLKNRTGAGVPPYELLKPFSGPGVTGKG 193
           W        AFE+F   +K ++  ID RN D  LKNRTG    PY LL P S  GVTG+G
Sbjct: 795 WAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRG 854

Query: 194 VPYSVSI 201
           +P SVSI
Sbjct: 855 IPNSVSI 859

The following BLAST results are available for this feature:

BLAST of CSPI04G13510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24370	2.4e-70	65.95	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO...	[more]
R9WS04	2.5e-67	63.78	Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=...	[more]
P38418	8.2e-63	61.08	Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1	[more]
Q8GSM2	1.0e-60	57.53	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1	[more]
P38419	3.3e-56	54.55	Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX...	[more]

BLAST of CSPI04G13510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DD89	1.8e-89	84.86	Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00030 P...	[more]
A0A6J1H2B7	2.4e-89	84.32	Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1	[more]
A0A6J1JXK9	6.9e-89	83.78	Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111490700 PE=3 SV=1	[more]
A0A125S6K8	1.2e-88	83.78	Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX13 PE=2 SV=1	[more]
A0A1S3BMS1	1.2e-88	83.78	Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491539 PE=3 SV=1	[more]

BLAST of CSPI04G13510 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK21641.1	3.7e-89	84.86	linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa]	[more]
KAG6605891.1	4.9e-89	84.32	Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma s...	[more]
XP_022958148.1	4.9e-89	84.32	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata]	[more]
XP_023532782.1	4.9e-89	84.32	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022995022.1	1.4e-88	83.78	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of CSPI04G13510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G45140.1	5.8e-64	61.08	lipoxygenase 2	[more]
AT1G67560.1	6.4e-47	47.87	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G72520.1	9.3e-46	48.39	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G17420.1	3.9e-44	47.85	lipoxygenase 3	[more]
AT1G55020.1	8.2e-42	44.92	lipoxygenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 14..183 e-value: 1.0E-67 score: 229.1
IPR013819	Lipoxygenase, C-terminal	PROSITE	PS51393	LIPOXYGENASE_3	coord: 1..200 score: 66.952232
None	No IPR available	GENE3D	1.20.245.10		coord: 8..199 e-value: 3.9E-58 score: 199.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR11771:SF89	LINOLEATE 13S-LIPOXYGENASE 2-1, CHLOROPLASTIC	coord: 14..198
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 14..198
IPR036226	Lipoxigenase, C-terminal domain superfamily	SUPERFAMILY	48484	Lipoxigenase	coord: 15..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G13510.1	CSPI04G13510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0034440	lipid oxidation
biological_process	GO:0031408	oxylipin biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen