CSPI04G13440 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G13440
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionLipoxygenase
LocationChr4: 11481104 .. 11484015 (+)
RNA-Seq ExpressionCSPI04G13440
SyntenyCSPI04G13440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCTGACAATTAAATTGAAATTTCAATCTATAAATATGGTAAGAGTTGTTGGTAGCTAAACACCATCACACAAGAAAGAGTATTACAGCAGCAGAGGCCATGGCGTCTAACATCATTGCAATTGTTTCTGTGATACCCAAAATCAATGAAACGTTTCCTTCTGATGATACTCTCTTGCTCTTCTTTGCTAGCTCAGATTTGGACAGAGGTAAAAAATAAGAAAAAGAATGCAAACTTTTTTGTTAATTTGTGTTGTATTGTATAGTATTGATCGTATGTTAAAATTAATGGATTGGTAATACTGTTCTTGATCAGATGGGCAGCAAAAACCATTGATCGGAGGTGTGGCAGAATTAATCAAGAATGAATCTACAGCAGAAAAGTACAAATACGTTGCTAAAATTCAAGTTCCAAAAGGTTTTGGAGAGATTGGAGCTGTGATTGTTGAATTGAAAGACGACTCGTCTGAGAAATTTATCGATACTGTTATTGTTGCAAATCCAACATCTCATAACACAATTACGTTCAGCTGTACCTCATGGGTTCAACCCAAAAGTCTCATTCCTGACCAACGCAGGGTTTTCTTTTCCACGAAGGTTCGTTGGTTCATATATATATATATATATATATATATATTGTTGTGTAATGATTATTACGAGAGATGATTCAAATTAATGTATTGTGTTGCTAATAATTGAATATGGAATAATATGAGCAGTCCTATTTGCCCGGAGATACGCCAGCAGGGCTGCTGAAGTTGAGAGCAGAAGATCTCGAGAATTTGAGAGGCCAAAAAGAAGATGGAACAGTGGATACAAATGAGCGTAAGGCGTTCGAAAGGATTTACGATTACGACGTGTACAATGACCTCGGAGATCCCGACGTAGACACCAAATGGAAAAGACCAGTTCTTGGTGGTTCAGTTCAGTATCCTTACCCCCGACGTTGTAGAACTGGTCGTCCTGCTACCGTATCAGGTATGTATATATACACTTCTCTTGTTTCTATATCATACATTTTCATTATGGAGTTTGCAAAAATAGCAAAAAAATTACGATAACAGAGTCCATCTCAATTACTTTTTCAAAATTGTAAAAATAACAAATTTTAAAAGTAGAATATCGTCGATTACTGTCATATTTGCCAAATTTGCAATATGAAAAAAAAAATGTTATGAGCTATTTTTTCTAAAAAAATTTTGTTCCTTCTCGTTAAAGTTTTTCTTTTTCATGGTTTTTTTTTTACTTATATTTTCAAATTCTCATAGTTTACGTTTTAGTTTATTTTGATTTTTATACATTTTGTCTAAAGTTTTTAAAATTGGACCAACTTGATTTCTTAAAAATAAAAATATGAATGGGATGAATCATTGAATGAAAGTTGAAAAATAAAAAATTTGGTATTTTTAAATATAGCAAAATAAATAAAAATATTTCAAAATATTCCTATCTATTACTTACATACACTAATAGTATTTAAATTAAGGGTAATTATAACGAGCAACAACTTTAAAAATAATAATTAAGTATATAGTAACATTTTTTTTAAAAAAATTATAAATATAGTCCACCAAACCAATATTAGAGCAACATAGAGATTGATTTTGGTATGTGGGTAACTTTTTAAAATGTTGGTATATACTTTATTTTGCAACTCTTCCTTCAATTAAAGCAACATTTTGTTGTCCACTTACATTATACATAAAAATAAAAACTCAAAAAATATTTTTAAGAATAAGAAAATAAGAGTTGGCATTTAGTTAATAATATAAAAGAAAATTAAACATATAGTTACTTGAAATTAAATATAATAATGTTTTTGGGAAGTAATTAACAAATGATACAATTGTGGTTAGATCCAAAGTCAGAGCAAAGAAGTACAGAAAGCTTTTATGTTCCAAGAGATGAAGCATTCTCAGAAGTTAAGCAGAAAATGTTCCCATCAAACCCAGGGAAGAAAGATAAGTTAGGAGCAAAGCCTTTCACCGACTTTACTCAGATTGACTTAATGTTCAGAGACGGCATTGCACCTCCATCCAATCCACATGAAATTCTTCAATTTAACATTATCTCCACACTTAATGCATCAAATCAACCCGCCCCACCTTCTGTTCAGCCCACTCCTGTTCAACCCGACACCCCTGTAATCAAATTCCCACCACCGGAGGCTCTCAAAAGTAAGCACCATACATACACAGTACATAAACATAACTTTTCTTTTTCTTTTTCTTTTTTGTAAGCAATTATTAGTACAACTTAATTACTTCATTTACCTCACAATACGTAATCCTTGTGTTTTTCCAGGAGACAAGTTCAATTGGCTTAGTGACAGTGAATTTGCAAGACAAACAATTGCGGGTCTTAATCCCTATTGTATACAGTTGGTCAAGGTGAGTGACAGACTAACAATAATCAATAAATATGTATCAAACTACCCTTACTCACTAGCTTTAATTAAAACCAAGTCAAATATTAGGGATTTAAGACAGATATTTTTAATCTGCTCTCTATAGTATATATGAAATTATAGCTTAATTATTTTGTATGAATAAATTCAGAGTTGGCCTTTGAAGAGTGAGCTGGACCCCGTGGAATATGGTCCTCAAGAATCAGCATTCACCACGGAATTGGTTCAAAAATTAATAGGAAGTTCCATCACAGTTGAAGAGGCTATAGCGCAAAAGAAATTGTTCGTATTGGATTACCATGATACTTTGATGCAATATGTAGAGAAAGTAAGAAGCATTAAGCGAACCACACTCTATGGATCAAGAACCTTGTTCTTCCTAAACGATGATGACACTTTGACTCCCTTGGGTATTGAGCTAACTCGGCCACCAATGGCTGGAAAACCCCAATGGAAACAAGTTTTCTCACCAAGCACTCAAGCGAAAAAACAAACACAAATTTAA

mRNA sequence

TTCCTGACAATTAAATTGAAATTTCAATCTATAAATATGGTAAGAGTTGTTGGTAGCTAAACACCATCACACAAGAAAGAGTATTACAGCAGCAGAGGCCATGGCGTCTAACATCATTGCAATTGTTTCTGTGATACCCAAAATCAATGAAACGTTTCCTTCTGATGATACTCTCTTGCTCTTCTTTGCTAGCTCAGATTTGGACAGAGATGGGCAGCAAAAACCATTGATCGGAGGTGTGGCAGAATTAATCAAGAATGAATCTACAGCAGAAAAGTACAAATACGTTGCTAAAATTCAAGTTCCAAAAGGTTTTGGAGAGATTGGAGCTGTGATTGTTGAATTGAAAGACGACTCGTCTGAGAAATTTATCGATACTGTTATTGTTGCAAATCCAACATCTCATAACACAATTACGTTCAGCTGTACCTCATGGGTTCAACCCAAAAGTCTCATTCCTGACCAACGCAGGGTTTTCTTTTCCACGAAGTCCTATTTGCCCGGAGATACGCCAGCAGGGCTGCTGAAGTTGAGAGCAGAAGATCTCGAGAATTTGAGAGGCCAAAAAGAAGATGGAACAGTGGATACAAATGAGCGTAAGGCGTTCGAAAGGATTTACGATTACGACGTGTACAATGACCTCGGAGATCCCGACGTAGACACCAAATGGAAAAGACCAGTTCTTGGTGGTTCAGTTCAGTATCCTTACCCCCGACGTTGTAGAACTGGTCGTCCTGCTACCGTATCAGATCCAAAGTCAGAGCAAAGAAGTACAGAAAGCTTTTATGTTCCAAGAGATGAAGCATTCTCAGAAGTTAAGCAGAAAATGTTCCCATCAAACCCAGGGAAGAAAGATAAGTTAGGAGCAAAGCCTTTCACCGACTTTACTCAGATTGACTTAATGTTCAGAGACGGCATTGCACCTCCATCCAATCCACATGAAATTCTTCAATTTAACATTATCTCCACACTTAATGCATCAAATCAACCCGCCCCACCTTCTGTTCAGCCCACTCCTGTTCAACCCGACACCCCTGTAATCAAATTCCCACCACCGGAGGCTCTCAAAAGAGACAAGTTCAATTGGCTTAGTGACAGTGAATTTGCAAGACAAACAATTGCGGGTCTTAATCCCTATTGTATACAGTTGGTCAAGAGTTGGCCTTTGAAGAGTGAGCTGGACCCCGTGGAATATGGTCCTCAAGAATCAGCATTCACCACGGAATTGGTTCAAAAATTAATAGGAAGTTCCATCACAGTTGAAGAGGCTATAGCGCAAAAGAAATTGTTCGTATTGGATTACCATGATACTTTGATGCAATATGTAGAGAAAGTAAGAAGCATTAAGCGAACCACACTCTATGGATCAAGAACCTTGTTCTTCCTAAACGATGATGACACTTTGACTCCCTTGGGTATTGAGCTAACTCGGCCACCAATGGCTGGAAAACCCCAATGGAAACAAGTTTTCTCACCAAGCACTCAAGCGAAAAAACAAACACAAATTTAA

Coding sequence (CDS)

ATGGCGTCTAACATCATTGCAATTGTTTCTGTGATACCCAAAATCAATGAAACGTTTCCTTCTGATGATACTCTCTTGCTCTTCTTTGCTAGCTCAGATTTGGACAGAGATGGGCAGCAAAAACCATTGATCGGAGGTGTGGCAGAATTAATCAAGAATGAATCTACAGCAGAAAAGTACAAATACGTTGCTAAAATTCAAGTTCCAAAAGGTTTTGGAGAGATTGGAGCTGTGATTGTTGAATTGAAAGACGACTCGTCTGAGAAATTTATCGATACTGTTATTGTTGCAAATCCAACATCTCATAACACAATTACGTTCAGCTGTACCTCATGGGTTCAACCCAAAAGTCTCATTCCTGACCAACGCAGGGTTTTCTTTTCCACGAAGTCCTATTTGCCCGGAGATACGCCAGCAGGGCTGCTGAAGTTGAGAGCAGAAGATCTCGAGAATTTGAGAGGCCAAAAAGAAGATGGAACAGTGGATACAAATGAGCGTAAGGCGTTCGAAAGGATTTACGATTACGACGTGTACAATGACCTCGGAGATCCCGACGTAGACACCAAATGGAAAAGACCAGTTCTTGGTGGTTCAGTTCAGTATCCTTACCCCCGACGTTGTAGAACTGGTCGTCCTGCTACCGTATCAGATCCAAAGTCAGAGCAAAGAAGTACAGAAAGCTTTTATGTTCCAAGAGATGAAGCATTCTCAGAAGTTAAGCAGAAAATGTTCCCATCAAACCCAGGGAAGAAAGATAAGTTAGGAGCAAAGCCTTTCACCGACTTTACTCAGATTGACTTAATGTTCAGAGACGGCATTGCACCTCCATCCAATCCACATGAAATTCTTCAATTTAACATTATCTCCACACTTAATGCATCAAATCAACCCGCCCCACCTTCTGTTCAGCCCACTCCTGTTCAACCCGACACCCCTGTAATCAAATTCCCACCACCGGAGGCTCTCAAAAGAGACAAGTTCAATTGGCTTAGTGACAGTGAATTTGCAAGACAAACAATTGCGGGTCTTAATCCCTATTGTATACAGTTGGTCAAGAGTTGGCCTTTGAAGAGTGAGCTGGACCCCGTGGAATATGGTCCTCAAGAATCAGCATTCACCACGGAATTGGTTCAAAAATTAATAGGAAGTTCCATCACAGTTGAAGAGGCTATAGCGCAAAAGAAATTGTTCGTATTGGATTACCATGATACTTTGATGCAATATGTAGAGAAAGTAAGAAGCATTAAGCGAACCACACTCTATGGATCAAGAACCTTGTTCTTCCTAAACGATGATGACACTTTGACTCCCTTGGGTATTGAGCTAACTCGGCCACCAATGGCTGGAAAACCCCAATGGAAACAAGTTTTCTCACCAAGCACTCAAGCGAAAAAACAAACACAAATTTAA

Protein sequence

MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQAKKQTQI*
Homology
BLAST of CSPI04G13440 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.7e-98
Identity = 206/452 (45.58%), Postives = 268/452 (59.29%), Query Frame = 0

Query: 24  TLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVELK 83
           + LL   SSDLD  G++K  +   A   + +  ++ YKY  + +VP  FGEIGAV+V+  
Sbjct: 106 SFLLELVSSDLDSSGKEKDTVKAYATYDELDKESKLYKYQCEFEVPDDFGEIGAVLVQ-- 165

Query: 84  DDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLK 143
              +E+  D   V N      +TF+C SW+  K   PD +R+FF  KSYLP +TP GL  
Sbjct: 166 ---NERHRD-AYVKNIVLDEIVTFTCDSWIHSKFDNPD-KRIFFLNKSYLPSETPEGLKS 225

Query: 144 LRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPY 203
           LR +DLE+LRG  E       ER++F+RIYDYD YND+GDPD D+   RPVLGG+ ++P+
Sbjct: 226 LRQKDLESLRGNGE------GERQSFDRIYDYDTYNDIGDPDTDSDMARPVLGGN-EHPF 285

Query: 204 PRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQ------KMFPSNPGKKDKLGA- 263
           PRRCRTGR  T ++P SE R+T  FYVPRDE F+E+KQ       ++    G    L + 
Sbjct: 286 PRRCRTGRKMTSTEPWSESRTTLPFYVPRDEDFAEIKQITRGATTLYSVLHGVIPALSSV 345

Query: 264 -----KPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSVQPTPVQPDT 323
                K F  F  I+L++  G+     P            ++    A P +         
Sbjct: 346 LKDEDKGFPLFRDIELLYEKGVDIDPPP------------DSGTLSALPRLVKAITNSTK 405

Query: 324 PVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESA 383
            V++F  P    +D F+W  D EF RQT+AGLNPY IQLV  WPL S+LDP  YGP ESA
Sbjct: 406 KVLQFETPRIKHKDSFSWFRDEEFCRQTLAGLNPYSIQLVTEWPLMSKLDPEVYGPAESA 465

Query: 384 FTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLND 443
            T E V++ I   +T EEA+ QK+LF+LDYHD L+ YV KVR I+ TTLYGSRTL FL  
Sbjct: 466 ITKETVEEEIKGFMTXEEALEQKRLFLLDYHDLLLPYVNKVREIEGTTLYGSRTLMFLTC 525

Query: 444 DDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
             TL PL IELTRPP  GKPQWK V++P   A
Sbjct: 526 TGTLRPLAIELTRPPNNGKPQWKHVYTPCWDA 531

BLAST of CSPI04G13440 vs. ExPASy Swiss-Prot
Match: P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 4.8e-98
Identity = 205/461 (44.47%), Postives = 277/461 (60.09%), Query Frame = 0

Query: 24  TLLLFFASSDLD-RDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIV-- 83
           +L L   SS+LD + G++K  +   A  + ++  +    Y A   VP GFG IGA+IV  
Sbjct: 113 SLSLELVSSELDAKTGKEKATVRSYAHNVDDDDHS-VVTYEADFDVPSGFGPIGAIIVTN 172

Query: 84  ELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIPD---QRRVFFSTKSYLPGDT 143
           EL+ +   + I+        +   +   C SWVQPKS+  +    +R+FF+ K+YLPG T
Sbjct: 173 ELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANKTYLPGQT 232

Query: 144 PAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGG 203
           PAGL   R  DL+  RG   DG   T ER+A +R+YDYDVYNDLG+PD +    RPVLGG
Sbjct: 233 PAGLRSYRKNDLQQKRG---DG---TGEREADDRVYDYDVYNDLGNPDSNGDLARPVLGG 292

Query: 204 SVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQKMF----------PSN 263
           + Q+PYPRRCRTGRP +  DPKSE R   + YVPRDE FS  K+  F           + 
Sbjct: 293 NKQFPYPRRCRTGRPPSKKDPKSETRK-GNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAV 352

Query: 264 PGKKDKLGAK-----PFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSV 323
           P  +  L  K     PF  F  ID +F DG+  P           +  LN      P  +
Sbjct: 353 PAAQSLLLDKLKWNLPFPSFFVIDKLFEDGVELPG----------VDKLNFLESVVPRLL 412

Query: 324 QPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDP 383
           +     P   +++F  P  +++DKF WL D EFAR+T+AG+NPY I+LV+ +PLKS+LDP
Sbjct: 413 EHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDP 472

Query: 384 VEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLYG 443
             YGP ESA T +L+++ +   +TVEEAI+QK+LF+LD+HD  + YV K+RS+  TT+YG
Sbjct: 473 AVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLDHTTMYG 532

Query: 444 SRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           SRT+FFL DD TL  L IELTRP    +PQW+QVF+PST A
Sbjct: 533 SRTVFFLTDDGTLQLLAIELTRPASPSQPQWRQVFTPSTDA 555

BLAST of CSPI04G13440 vs. ExPASy Swiss-Prot
Match: Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.1e-97
Identity = 208/454 (45.81%), Postives = 278/454 (61.23%), Query Frame = 0

Query: 24  TLLLFFASSDLD-RDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVEL 83
           TLLL   SS+LD + G+++  + G A +   E T     Y AK+ VP  FG +GAV+VE 
Sbjct: 100 TLLLELVSSELDPKTGRERERVKGFAHMTLKEGT-----YEAKMSVPASFGPVGAVLVE- 159

Query: 84  KDDSSEKFI-DTVIVANPTSHNTITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGL 143
            +   E FI D  ++        ITF   SWV  K   P+  R FF+ KSYLP  TP G+
Sbjct: 160 NEHHREMFIKDIKLITGGDESTAITFDVASWVHSKFDDPEP-RAFFTVKSYLPSQTPPGI 219

Query: 144 LKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQY 203
             LR ++LE LRG   DG    +ERK  ER+YDYD YNDLGDPD +   KRPVL G+ ++
Sbjct: 220 EALRKKELETLRG---DG---HSERKFHERVYDYDTYNDLGDPDKNIDHKRPVL-GTKEH 279

Query: 204 PYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQKMFPSNPGKKDKLGAKP--- 263
           PYPRRCRTGRP T+ DP++E RS+   YVPRDE FS+VK + F +   +       P   
Sbjct: 280 PYPRRCRTGRPKTLYDPETETRSS-PVYVPRDEQFSDVKGRTFSATTLRSGLHAILPAVA 339

Query: 264 --------FTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSVQPTPVQPD 323
                   F+ F  ID ++ DGI  P + H    FN+I      N   P  VQ   ++  
Sbjct: 340 PLLNNSHGFSHFPAIDALYSDGIPLPVDGHGGNSFNVI------NDVIPRVVQ--MIEDT 399

Query: 324 TP-VIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQE 383
           T  V++F  PE L+RD+F+W  D EFARQT+AGLNP CI+ +  +P+ S+LDP  YGP E
Sbjct: 400 TEHVLRFEVPEMLERDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAE 459

Query: 384 SAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFL 443
           SA + E+++K++   +TVEEA+ +K+LF+LDYHD  + YV +VR +  TTLYGSRT+FFL
Sbjct: 460 SALSKEILEKMMNGRMTVEEAMEKKRLFLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFL 519

Query: 444 NDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           +D+ TL PL IELTRP    KPQWK+ F+  + A
Sbjct: 520 SDEGTLMPLAIELTRPQSPTKPQWKRAFTHGSDA 530

BLAST of CSPI04G13440 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.4e-96
Identity = 206/464 (44.40%), Postives = 275/464 (59.27%), Query Frame = 0

Query: 24  TLLLFFASSDLD-RDGQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVEL 83
           +L L   SS+L+ + G++K  +   A  + ++       Y A   VP GFG IGAV+V  
Sbjct: 131 SLFLELVSSELEAKTGKKKATVHSYAHKVDDDDHG-VVTYEADFDVPTGFGPIGAVVV-T 190

Query: 84  KDDSSEKFI-DTVIVANPTSHNT--ITFSCTSWVQPKSLIPD---QRRVFFSTKSYLPGD 143
            +   E F+ D  + A   + N+  +   C SWVQPKS I +    +R+FF+ K+YLPG 
Sbjct: 191 NELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFFA-KAYLPGQ 250

Query: 144 TPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLG 203
           TPAGL   R EDL+  RG          +R+A +R+YDYDVYNDLG+PD +    RPVLG
Sbjct: 251 TPAGLRSYREEDLKQKRGN------GAGQREADDRVYDYDVYNDLGNPDSNGDLARPVLG 310

Query: 204 GSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVKQKMF------------ 263
           GS Q+PYPRRCRTGRP +  DPKSE R   + YVPRDE FSEVK   F            
Sbjct: 311 GSKQFPYPRRCRTGRPPSKKDPKSETRK-GNVYVPRDEEFSEVKNAQFLLKTLQSVLHAA 370

Query: 264 --PSNPGKKDKLGAK-PFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPS 323
              +     D L    PF  F  ID +F DG+  P           +  L   +   P  
Sbjct: 371 VPAAQSALIDNLSLNLPFPSFFVIDKLFEDGVELPG----------VEKLGFLHSIVPRL 430

Query: 324 VQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELD 383
           ++     P   ++ F  P  +++DKF WL D EFAR+T+AG+NPY I+LV+ +PLKS+LD
Sbjct: 431 LELLRDSPGDKILLFDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLD 490

Query: 384 PVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLY 443
           P  YGP ESA T +L+++ +   +TVEEAI+QK+LF+LD+HD  + YV K+RS+K TT+Y
Sbjct: 491 PAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLKHTTMY 550

Query: 444 GSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQAKK 466
           GSRT+FFL DD TL  L IELTRP    +PQW+QVF+PST   K
Sbjct: 551 GSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFTPSTDTTK 574

BLAST of CSPI04G13440 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.7e-95
Identity = 212/483 (43.89%), Postives = 283/483 (58.59%), Query Frame = 0

Query: 2   ASNIIAIVSVIPKINETFPS--DD-------TLLLFFASSDLD-RDGQQKPLIGGVAELI 61
           A+ + A+V+V  ++N       DD       +LLL+  +++LD + G +KP I   A   
Sbjct: 69  ATGVKAVVTVQKQVNLNLSRGLDDIGDLLGKSLLLWIVAAELDHKTGIEKPGIRAYAH-- 128

Query: 62  KNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTS 121
           +         Y A   +P+ FGE+GA+++E  +   E ++  +++ +   H  +  +C S
Sbjct: 129 RGRDVDGDTHYEADFVIPQDFGEVGAILIE-NEHHKEMYVKNIVI-DGFVHGKVEITCNS 188

Query: 122 WVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFER 181
           WV  K   PD +R+FF+ KSYLP  TP+G+ +LR E+L  LRG   DG     ERK FER
Sbjct: 189 WVHSKFDNPD-KRIFFTNKSYLPSQTPSGVSRLREEELVTLRG---DG---IGERKVFER 248

Query: 182 IYDYDVYNDLGDPDVDT-KWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYV 241
           IYDYDVYNDLG+ D +    KRPVLGG  + PYPRRC+TGRP +  DP SE RST   YV
Sbjct: 249 IYDYDVYNDLGEADSNNDDAKRPVLGGK-ELPYPRRCKTGRPRSKKDPLSETRST-FVYV 308

Query: 242 PRDEAFSEVKQKMFPSNPGKKDKLGAKP------------FTDFTQIDLMFRDGI-APPS 301
           PRDEAFSEVK   F  N          P            F  F  ID +F  G+  P  
Sbjct: 309 PRDEAFSEVKSVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNVGVDLPGL 368

Query: 302 NPHEILQFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFAR 361
              +   FN++           P +          V+ F  P+ ++RDKF+W  D EFAR
Sbjct: 369 GDKKSGLFNVV-----------PRLIKAISDTRKDVLLFESPQLVQRDKFSWFRDVEFAR 428

Query: 362 QTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLF 421
           QT+AGLNPY I+LV  WPL+S+LDP  YGP ES  T EL++K IG+ +TVE+A+ QKKLF
Sbjct: 429 QTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQQKKLF 488

Query: 422 VLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVF 461
           +LDYHD L+ YV KV  +K + LYGSRT+FFL    TL PL IELTRPP+  KPQWK+V+
Sbjct: 489 ILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQWKEVY 527

BLAST of CSPI04G13440 vs. ExPASy TrEMBL
Match: A0A5D3DDX5 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00120 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.4e-249
Identity = 431/463 (93.09%), Postives = 444/463 (95.90%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA+NIIAI SVIPKINE FPSDDTL L FAS DLD DG  KPLIGG AELIKNEST +KY
Sbjct: 1   MAANIIAIASVIPKINEKFPSDDTLFLQFASCDLDADGLLKPLIGGEAELIKNESTEQKY 60

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYVAKI+VPKGFGEIGAVIVELKDDS EKFIDTV+VANPTSHNTITFSCTSWVQPK+LIP
Sbjct: 61  KYVAKIEVPKGFGEIGAVIVELKDDSPEKFIDTVVVANPTSHNTITFSCTSWVQPKNLIP 120

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           DQRR+FFSTKSYLP +TPAGL+KLRAEDLENLRGQKEDGTVDTN+RKAFERIYDYDVYND
Sbjct: 121 DQRRIFFSTKSYLPEETPAGLVKLRAEDLENLRGQKEDGTVDTNQRKAFERIYDYDVYND 180

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRS ESFYVPRDEAFSEVK
Sbjct: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSAESFYVPRDEAFSEVK 240

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ   P
Sbjct: 241 QKMFPNNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ---P 300

Query: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360
           SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQTIAGLNPYCIQLVKSWPLKSEL
Sbjct: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTIAGLNPYCIQLVKSWPLKSEL 360

Query: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420
           DP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHDTLMQYVEKVRSIKRTTL
Sbjct: 361 DPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHDTLMQYVEKVRSIKRTTL 420

Query: 421 YGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           YGSRTLFFLN DDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA
Sbjct: 421 YGSRTLFFLNSDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 460

BLAST of CSPI04G13440 vs. ExPASy TrEMBL
Match: A0A5A7TH32 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00110 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.7e-245
Identity = 430/480 (89.58%), Postives = 443/480 (92.29%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRD-----------------GQQKPL 60
           MA+NIIAI SVIPKINE FPSDDTL L FAS DLD                   G  KPL
Sbjct: 1   MAANIIAIASVIPKINEKFPSDDTLFLQFASCDLDAGKKKKKEEECNLFDDDFYGLLKPL 60

Query: 61  IGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHN 120
           IGG AELIKNEST +KYKYVAKI+VPKGFGEIGAVIVELKDDS EKFIDTV+VANPTSHN
Sbjct: 61  IGGEAELIKNESTEQKYKYVAKIEVPKGFGEIGAVIVELKDDSPEKFIDTVVVANPTSHN 120

Query: 121 TITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDT 180
           TITFSCTSWVQPK+LIPDQRR+FFSTKSYLP +TPAGL+KLRAEDLENLRGQKEDGTVDT
Sbjct: 121 TITFSCTSWVQPKNLIPDQRRIFFSTKSYLPEETPAGLVKLRAEDLENLRGQKEDGTVDT 180

Query: 181 NERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR 240
           N+RKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR
Sbjct: 181 NQRKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR 240

Query: 241 STESFYVPRDEAFSEVKQKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL 300
           S ESFYVPRDEAFSEVKQKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL
Sbjct: 241 SAESFYVPRDEAFSEVKQKMFPTNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL 300

Query: 301 QFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGL 360
           QFNIISTLNASNQ   PSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQTIAGL
Sbjct: 301 QFNIISTLNASNQ---PSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTIAGL 360

Query: 361 NPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHD 420
           NPYCIQLVKSWPLKSELDP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHD
Sbjct: 361 NPYCIQLVKSWPLKSELDPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHD 420

Query: 421 TLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           TLMQYVEKVRSIKRTTLYGSRTLFFLN DDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA
Sbjct: 421 TLMQYVEKVRSIKRTTLYGSRTLFFLNSDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 477

BLAST of CSPI04G13440 vs. ExPASy TrEMBL
Match: A0A5A7TJ25 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00140 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.5e-243
Identity = 419/463 (90.50%), Postives = 437/463 (94.38%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA+NIIA  SVIPKINE FPSDD+L   FAS DLD DGQ KP+IGG A+LIKNEST +KY
Sbjct: 1   MAANIIAFASVIPKINEKFPSDDSLFFLFASCDLDADGQLKPMIGGEAQLIKNESTEQKY 60

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYV KI+VPKGFGEIGAVIVELKD S EKFI+TV+VANPTSHNTITFSCTSWVQPK+LIP
Sbjct: 61  KYVVKIEVPKGFGEIGAVIVELKDGSPEKFINTVVVANPTSHNTITFSCTSWVQPKNLIP 120

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           +QRR+FFSTKSYLP +TPAGL+ LRAEDL NLRGQKEDGTVDTNERKAFERIYDYDVYND
Sbjct: 121 NQRRIFFSTKSYLPEETPAGLVNLRAEDLANLRGQKEDGTVDTNERKAFERIYDYDVYND 180

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRS ESFYVPRDEAFSEVK
Sbjct: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSAESFYVPRDEAFSEVK 240

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ    
Sbjct: 241 QKMFPTNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ---S 300

Query: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360
           SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQT+AGLNPYCIQLVKSWPLKSEL
Sbjct: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTLAGLNPYCIQLVKSWPLKSEL 360

Query: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420
           DP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHDTLMQYVEKVRSIKRTTL
Sbjct: 361 DPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHDTLMQYVEKVRSIKRTTL 420

Query: 421 YGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           YGSRTLFFLN DDTLTPLGIELTRPP AGKPQWKQVFSPSTQA
Sbjct: 421 YGSRTLFFLNSDDTLTPLGIELTRPPKAGKPQWKQVFSPSTQA 460

BLAST of CSPI04G13440 vs. ExPASy TrEMBL
Match: A0A6J1J654 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483812 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.5e-214
Identity = 378/464 (81.47%), Postives = 413/464 (89.01%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA +IIA+++V PK+NE FPSDDTL L FASSDLD DGQQKPL+GGVA  I+NES AE+Y
Sbjct: 10  MAVDIIAVITVKPKLNEKFPSDDTLSLKFASSDLDTDGQQKPLLGGVA-TIQNES-AEEY 69

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYVAKIQVPKG+GEIGAVI+ELK++S+E+FIDTV +AN +SHN++TFSCTSWVQPK+LIP
Sbjct: 70  KYVAKIQVPKGYGEIGAVIIELKENSTERFIDTVSIANSSSHNSVTFSCTSWVQPKNLIP 129

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           DQRR+FFSTKSYLPG+TPAGL+KLRAEDL NLRGQK DGT D NERK FERIYDYDVYND
Sbjct: 130 DQRRIFFSTKSYLPGNTPAGLVKLRAEDLANLRGQKPDGTEDKNERKGFERIYDYDVYND 189

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVD KWKRPVLGGS QYPYPRRCRTGRPA VSDP SE R  ESFYVPRDEAFSEVK
Sbjct: 190 LGDPDVDMKWKRPVLGGSAQYPYPRRCRTGRPAAVSDPSSEHRGEESFYVPRDEAFSEVK 249

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLG+KPF+DFTQIDLMFRDGI  P  PHEIL+FNI STL  S   APP
Sbjct: 250 QKMFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTL--STMHAPP 309

Query: 301 SVQPTPVQPD-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSE 360
            V P+ +QP    ++KFPPPEALKRDKFNWLSDSEFARQT+AGLNPY IQLVKS PL SE
Sbjct: 310 FVDPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSE 369

Query: 361 LDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTT 420
           L+P +YGPQES FTT+LVQKLIG SITVEEA+AQKKLFVLDYHDTLMQYV KVRSIKRTT
Sbjct: 370 LNPKDYGPQESGFTTQLVQKLIGCSITVEEALAQKKLFVLDYHDTLMQYVTKVRSIKRTT 429

Query: 421 LYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           LYGSRTLFFLN DDTL PLGIELTRPPM GKPQWKQVFSPST A
Sbjct: 430 LYGSRTLFFLNSDDTLRPLGIELTRPPMDGKPQWKQVFSPSTHA 469

BLAST of CSPI04G13440 vs. ExPASy TrEMBL
Match: A0A6J1FUE7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.6e-213
Identity = 381/464 (82.11%), Postives = 411/464 (88.58%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA +I+A+V+V PK NE FPSDDTL L FASSDLD DGQQKPLIGGVA  I+NES AE+Y
Sbjct: 14  MAVDIVAVVTVRPKFNEKFPSDDTLSLKFASSDLDADGQQKPLIGGVA-TIQNES-AEEY 73

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
            YVAKIQVP+GFGEIGAVI+ELKD+S+E+FIDTV VAN +S N++TFSCTSWVQPK+LIP
Sbjct: 74  TYVAKIQVPRGFGEIGAVIIELKDNSTERFIDTVSVANSSSQNSVTFSCTSWVQPKNLIP 133

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           DQRR+FFSTKSYLPG+TPAGL+KLRAEDL NLRGQK DGT D NERKAFERIYDYDVYND
Sbjct: 134 DQRRIFFSTKSYLPGNTPAGLVKLRAEDLANLRGQKPDGTEDENERKAFERIYDYDVYND 193

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVD KWKRPVLGGS QYPYPRRCRTGRPA VSDP SE R  ESFYVPRDEAFSEVK
Sbjct: 194 LGDPDVDMKWKRPVLGGSAQYPYPRRCRTGRPAAVSDPWSEHRGEESFYVPRDEAFSEVK 253

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLG+KPF+DFTQIDLMFRDGI  P  PHEIL+FNI STL  S   APP
Sbjct: 254 QKMFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTL--STMHAPP 313

Query: 301 SVQPTPVQPD-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSE 360
            V P+ +QP    ++KFPPPEALKRDKFNWLSDSEFARQT+AGLNPY IQLVKS PL SE
Sbjct: 314 FVDPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSE 373

Query: 361 LDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTT 420
           L+P +YGPQES FTTELVQKLIG SITVEEA+A KKLFVLDYHDTLMQYV KVRSIKRTT
Sbjct: 374 LNPKDYGPQESGFTTELVQKLIGCSITVEEALAHKKLFVLDYHDTLMQYVTKVRSIKRTT 433

Query: 421 LYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           LYGSRTLFFLN DDTLTPLGIELTRPPM GKPQWKQVFSPST A
Sbjct: 434 LYGSRTLFFLNSDDTLTPLGIELTRPPMDGKPQWKQVFSPSTHA 473

BLAST of CSPI04G13440 vs. NCBI nr
Match: XP_004142240.3 (linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KAE8649493.1 hypothetical protein Csa_017995 [Cucumis sativus])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 462/463 (99.78%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY
Sbjct: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP
Sbjct: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND
Sbjct: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK
Sbjct: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP
Sbjct: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300

Query: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360
           SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL
Sbjct: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360

Query: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420
           DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL
Sbjct: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420

Query: 421 YGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           YGSRTLFFLNDDDTL+PLGIELTRPPMAGKPQWKQVFSPSTQA
Sbjct: 421 YGSRTLFFLNDDDTLSPLGIELTRPPMAGKPQWKQVFSPSTQA 463

BLAST of CSPI04G13440 vs. NCBI nr
Match: TYK21650.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 870.9 bits (2249), Expect = 4.9e-249
Identity = 431/463 (93.09%), Postives = 444/463 (95.90%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA+NIIAI SVIPKINE FPSDDTL L FAS DLD DG  KPLIGG AELIKNEST +KY
Sbjct: 1   MAANIIAIASVIPKINEKFPSDDTLFLQFASCDLDADGLLKPLIGGEAELIKNESTEQKY 60

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYVAKI+VPKGFGEIGAVIVELKDDS EKFIDTV+VANPTSHNTITFSCTSWVQPK+LIP
Sbjct: 61  KYVAKIEVPKGFGEIGAVIVELKDDSPEKFIDTVVVANPTSHNTITFSCTSWVQPKNLIP 120

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           DQRR+FFSTKSYLP +TPAGL+KLRAEDLENLRGQKEDGTVDTN+RKAFERIYDYDVYND
Sbjct: 121 DQRRIFFSTKSYLPEETPAGLVKLRAEDLENLRGQKEDGTVDTNQRKAFERIYDYDVYND 180

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRS ESFYVPRDEAFSEVK
Sbjct: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSAESFYVPRDEAFSEVK 240

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ   P
Sbjct: 241 QKMFPNNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ---P 300

Query: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360
           SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQTIAGLNPYCIQLVKSWPLKSEL
Sbjct: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTIAGLNPYCIQLVKSWPLKSEL 360

Query: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420
           DP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHDTLMQYVEKVRSIKRTTL
Sbjct: 361 DPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHDTLMQYVEKVRSIKRTTL 420

Query: 421 YGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           YGSRTLFFLN DDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA
Sbjct: 421 YGSRTLFFLNSDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 460

BLAST of CSPI04G13440 vs. NCBI nr
Match: KAA0041366.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 857.4 bits (2214), Expect = 5.6e-245
Identity = 430/480 (89.58%), Postives = 443/480 (92.29%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRD-----------------GQQKPL 60
           MA+NIIAI SVIPKINE FPSDDTL L FAS DLD                   G  KPL
Sbjct: 1   MAANIIAIASVIPKINEKFPSDDTLFLQFASCDLDAGKKKKKEEECNLFDDDFYGLLKPL 60

Query: 61  IGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHN 120
           IGG AELIKNEST +KYKYVAKI+VPKGFGEIGAVIVELKDDS EKFIDTV+VANPTSHN
Sbjct: 61  IGGEAELIKNESTEQKYKYVAKIEVPKGFGEIGAVIVELKDDSPEKFIDTVVVANPTSHN 120

Query: 121 TITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDT 180
           TITFSCTSWVQPK+LIPDQRR+FFSTKSYLP +TPAGL+KLRAEDLENLRGQKEDGTVDT
Sbjct: 121 TITFSCTSWVQPKNLIPDQRRIFFSTKSYLPEETPAGLVKLRAEDLENLRGQKEDGTVDT 180

Query: 181 NERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR 240
           N+RKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR
Sbjct: 181 NQRKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR 240

Query: 241 STESFYVPRDEAFSEVKQKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL 300
           S ESFYVPRDEAFSEVKQKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL
Sbjct: 241 SAESFYVPRDEAFSEVKQKMFPTNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEIL 300

Query: 301 QFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGL 360
           QFNIISTLNASNQ   PSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQTIAGL
Sbjct: 301 QFNIISTLNASNQ---PSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTIAGL 360

Query: 361 NPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHD 420
           NPYCIQLVKSWPLKSELDP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHD
Sbjct: 361 NPYCIQLVKSWPLKSELDPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHD 420

Query: 421 TLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           TLMQYVEKVRSIKRTTLYGSRTLFFLN DDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA
Sbjct: 421 TLMQYVEKVRSIKRTTLYGSRTLFFLNSDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 477

BLAST of CSPI04G13440 vs. NCBI nr
Match: KAA0041369.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 850.9 bits (2197), Expect = 5.2e-243
Identity = 419/463 (90.50%), Postives = 437/463 (94.38%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKINETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEKY 60
           MA+NIIA  SVIPKINE FPSDD+L   FAS DLD DGQ KP+IGG A+LIKNEST +KY
Sbjct: 1   MAANIIAFASVIPKINEKFPSDDSLFFLFASCDLDADGQLKPMIGGEAQLIKNESTEQKY 60

Query: 61  KYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIP 120
           KYV KI+VPKGFGEIGAVIVELKD S EKFI+TV+VANPTSHNTITFSCTSWVQPK+LIP
Sbjct: 61  KYVVKIEVPKGFGEIGAVIVELKDGSPEKFINTVVVANPTSHNTITFSCTSWVQPKNLIP 120

Query: 121 DQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYND 180
           +QRR+FFSTKSYLP +TPAGL+ LRAEDL NLRGQKEDGTVDTNERKAFERIYDYDVYND
Sbjct: 121 NQRRIFFSTKSYLPEETPAGLVNLRAEDLANLRGQKEDGTVDTNERKAFERIYDYDVYND 180

Query: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEVK 240
           LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRS ESFYVPRDEAFSEVK
Sbjct: 181 LGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSAESFYVPRDEAFSEVK 240

Query: 241 QKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPP 300
           QKMFP+NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ    
Sbjct: 241 QKMFPTNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQ---S 300

Query: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSEL 360
           SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSD EFARQT+AGLNPYCIQLVKSWPLKSEL
Sbjct: 301 SVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDGEFARQTLAGLNPYCIQLVKSWPLKSEL 360

Query: 361 DPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTL 420
           DP EYGPQESAFTT+LVQKLIGSS+TVEEAIAQKKLF+LDYHDTLMQYVEKVRSIKRTTL
Sbjct: 361 DPEEYGPQESAFTTDLVQKLIGSSLTVEEAIAQKKLFILDYHDTLMQYVEKVRSIKRTTL 420

Query: 421 YGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQA 464
           YGSRTLFFLN DDTLTPLGIELTRPP AGKPQWKQVFSPSTQA
Sbjct: 421 YGSRTLFFLNSDDTLTPLGIELTRPPKAGKPQWKQVFSPSTQA 460

BLAST of CSPI04G13440 vs. NCBI nr
Match: XP_011653576.2 (linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KAE8649492.1 hypothetical protein Csa_018105 [Cucumis sativus])

HSP 1 Score: 837.8 bits (2163), Expect = 4.6e-239
Identity = 420/463 (90.71%), Postives = 434/463 (93.74%), Query Frame = 0

Query: 1   MASNIIAIVSVIPKIN-ETFPSDDTLLLFFASSDLDRDGQQKPLIGGVAELIKNESTAEK 60
           MASNIIAIVSVIPK N      +D+LLLFFASSDLDRDGQQKPLI GVAELIKNEST  K
Sbjct: 1   MASNIIAIVSVIPKSNGSIIHYNDSLLLFFASSDLDRDGQQKPLIRGVAELIKNESTKAK 60

Query: 61  YKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLI 120
           +KYVAK+QVPKG+GEIGAV+VEL+  SSEKFIDTVIVAN TS NTITFSCTSWVQPKSLI
Sbjct: 61  HKYVAKVQVPKGYGEIGAVVVELETHSSEKFIDTVIVANSTSQNTITFSCTSWVQPKSLI 120

Query: 121 PDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYN 180
           PDQRR+FFSTKSYLP DTPAGLLKLRAEDLENLRGQK+DGTVDTNER  FERIYDYD+YN
Sbjct: 121 PDQRRIFFSTKSYLPEDTPAGLLKLRAEDLENLRGQKQDGTVDTNERTKFERIYDYDLYN 180

Query: 181 DLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTESFYVPRDEAFSEV 240
           DLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRP TVSDPKSEQRS +SFYVPRDEAFSEV
Sbjct: 181 DLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPHTVSDPKSEQRSAKSFYVPRDEAFSEV 240

Query: 241 KQKMFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAP 300
           KQ MFPSN G KDKLG KPFTDFTQIDLMFRDGIA PSNPHEILQFNI+STLNASNQPAP
Sbjct: 241 KQTMFPSNSGNKDKLGTKPFTDFTQIDLMFRDGIALPSNPHEILQFNILSTLNASNQPAP 300

Query: 301 PSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSE 360
           P VQP PVQP+TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSE
Sbjct: 301 PPVQPIPVQPNTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSE 360

Query: 361 LDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTT 420
           LDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTT
Sbjct: 361 LDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTT 420

Query: 421 LYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFSPSTQ 463
           LYGSRTLFFL  DDTLTP+GIELTRPPM GKPQWKQVFSPSTQ
Sbjct: 421 LYGSRTLFFLEYDDTLTPMGIELTRPPMDGKPQWKQVFSPSTQ 463

BLAST of CSPI04G13440 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 340.9 bits (873), Expect = 1.6e-93
Identity = 194/436 (44.50%), Postives = 258/436 (59.17%), Query Frame = 0

Query: 40  QKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANP 99
           Q+  +   A+ +  E+  EKY+   + ++P+ FG +GA+ ++      + F+  V +  P
Sbjct: 117 QRITVEDYAQRVWAEAPDEKYE--CEFEMPEDFGPVGAIKIQ-NQYHRQLFLKGVELKLP 176

Query: 100 TSHNTITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDG 159
               +ITF+C SWV PKS+ P  +R+FFS KSYLP  TP  L K R E+LE L+G+  + 
Sbjct: 177 --GGSITFTCESWVAPKSVDP-TKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNRE- 236

Query: 160 TVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPK 219
             +  E   FERIYDYDVYND+GDPD D +  RPV+GG + +PYPRRC+TGR    +DP 
Sbjct: 237 --EVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGG-LTHPYPRRCKTGRKPCETDPS 296

Query: 220 SEQRSTESFYVPRDEAFSEVKQKMFPSN----------PGKKDKL--GAKPFTDFTQIDL 279
           SEQR    FYVPRDE FS  K   F             P  +  L    +PF  F  I  
Sbjct: 297 SEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQN 356

Query: 280 MFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKF 339
           +F +GI  P               +A   P  P +     +    +++F  P  + RD+F
Sbjct: 357 LFEEGIQLPK--------------DAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRF 416

Query: 340 NWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITV 399
           +WL D EFARQT+AGLNPY IQLV+ WPL S+LDP  YG   S  T E+V++ +  ++TV
Sbjct: 417 SWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTV 476

Query: 400 EEAIAQKKLFVLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPM 459
           +EA+  K+LFVLDYHD L+ YV KVR +  TTLY SRTLFFL+DD TL P+ IELT PP 
Sbjct: 477 DEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPN 528

Query: 460 AGKPQWKQVFSPSTQA 464
             KPQWKQVF+P   A
Sbjct: 537 INKPQWKQVFTPGYDA 528

BLAST of CSPI04G13440 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 271.9 bits (694), Expect = 9.4e-73
Identity = 164/425 (38.59%), Postives = 245/425 (57.65%), Query Frame = 0

Query: 52  KNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTS 111
           K+++ AE+  Y A+  V   FG  GA+ V +     E F++++ +    +   + F C S
Sbjct: 149 KSKTKAERVHYTAEFTVDAAFGSPGAITV-MNKHQKEFFLESITIEG-FALGPVHFPCNS 208

Query: 112 WVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFER 171
           WVQ +   PD +R+FF+ + YLP +TP+GL  LR ++L+NLRG   DG   +  RK  +R
Sbjct: 209 WVQSQKDHPD-KRIFFTNQPYLPNETPSGLRVLREKELKNLRG---DG---SGVRKLSDR 268

Query: 172 IYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTE--SFY 231
           IYD+DVYNDLG+PD  ++  RP LGG  + PYPRRCRTGR +TVSD  +E R  +    Y
Sbjct: 269 IYDFDVYNDLGNPDKSSELSRPKLGGK-EVPYPRRCRTGRQSTVSDKDAESRVEKPLPMY 328

Query: 232 VPRDEAFSEVKQKMFPSN----------PGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNP 291
           VPRDE F E KQ  F +           P  K  + A+ F DF +ID ++++G+      
Sbjct: 329 VPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGL------ 388

Query: 292 HEILQFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQT 351
             +L+      +     P P  V  T  +    ++K+  P+ L +DK  WL D EFARQ 
Sbjct: 389 --LLKLGFQDDI-FKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQA 448

Query: 352 IAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTE-LVQKLIGSSITVEEAIAQKKLFV 411
           IAG+NP  I+ VK++P  S LDP  YGPQ SA T + ++  L G S  V++A+ + +L++
Sbjct: 449 IAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDGFS--VQQALEENRLYM 508

Query: 412 LDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQWKQVFS 464
           LDYHD  + +++++ ++     Y +RT+FFL    TL P+ IEL+ PP   K + K+V +
Sbjct: 509 LDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLPPHGPKHRSKRVLT 552

BLAST of CSPI04G13440 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-66
Identity = 171/444 (38.51%), Postives = 240/444 (54.05%), Query Frame = 0

Query: 25  LLLFFASSDLDRD--GQQKPLIGGVAELIKNESTAEKYKYVAKIQVPKGFGEIGAVIVEL 84
           +L+   S ++D +    +K L   V  L K         + A   VP  FG+ GA++V  
Sbjct: 115 MLIQLVSEEIDPETGKGRKSLESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILV-T 174

Query: 85  KDDSSEKFIDTVIVANPTSHNTITFSCTSWVQPKSLIPDQRRVFFSTKSYLPGDTPAGLL 144
              S+E  +  +I+ + T  +TI F   +W+  K+  P Q R+ F ++  LP +TP G+ 
Sbjct: 175 NLLSTEICLSEIIIEDST--DTILFPANTWIHSKNDNP-QARIIFRSQPCLPSETPDGIK 234

Query: 145 KLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYP 204
           +LR +DL ++RG   DG     ERK  ERIYDYDVYNDLGDP   T+  RPVL G  + P
Sbjct: 235 ELREKDLVSVRG---DG---KGERKPHERIYDYDVYNDLGDPR-KTERVRPVL-GVPETP 294

Query: 205 YPRRCRTGRPATVSDPKSEQR--STESFYVPRDEAFSEVKQKMF-------------PSN 264
           YPRRCRTGRP    DP  E R    E FYVPRDE F E+K+  F             PS 
Sbjct: 295 YPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSI 354

Query: 265 PGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPSVQPTPV 324
                 L   PFT F+ ID +++  I      H   +   +            +V  T +
Sbjct: 355 AAALSNLDI-PFTCFSDIDNLYKSNIV---LGHTEPKDTGLGGFIGGFMNGILNVTETLL 414

Query: 325 QPDTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELDPVEYGP 384
           + DTP +       +K D+F WL D+EF RQ +AG+NP  I+L+K  P++S LDP  YGP
Sbjct: 415 KYDTPAV-------IKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGP 474

Query: 385 QESAFTTELVQKLIGS-SITVEEAIAQKKLFVLDYHDTLMQYVEKVRSIKR--TTLYGSR 444
           QES  T E++ + +     T+E+A+ +K+LF++DYHD L+ +VEK+ SIK      Y SR
Sbjct: 475 QESVLTEEIIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASR 534

Query: 445 TLFFLNDDDTLTPLGIELTRPPMA 449
           T+FF + +  L PL IEL+ PP A
Sbjct: 535 TIFFYSKNGALRPLAIELSLPPTA 535

BLAST of CSPI04G13440 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-66
Identity = 154/427 (36.07%), Postives = 237/427 (55.50%), Query Frame = 0

Query: 52  KNESTAEKYKYVAKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTS 111
           K+ S AE+  Y A+  V   FG  GA+ V  K    E F++++ +    +   + F C S
Sbjct: 155 KSNSKAERVHYTAEFTVDSAFGSPGAITVTNK-HQKEFFLESITIEG-FACGPVHFPCNS 214

Query: 112 WVQPKSLIPDQRRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFER 171
           WVQ +   P  +R+ F+ + YLP +TP+GL  LR ++LENLRG  +       ERK  +R
Sbjct: 215 WVQSQKDHP-SKRILFTNQPYLPSETPSGLRTLREKELENLRGNGK------GERKLSDR 274

Query: 172 IYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQRSTE--SFY 231
           IYDYDVYND+G+PD+  +  RP LGG  ++PYPRRCRTGR +T +D  SE+R  +    Y
Sbjct: 275 IYDYDVYNDIGNPDISRELARPTLGGR-EFPYPRRCRTGRSSTDTDMMSERRVEKPLPMY 334

Query: 232 VPRDEAFSEVKQKMFPS----------NPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNP 291
           VPRDE F E KQ  F +           P  K  + A+ F +F +ID ++++G+      
Sbjct: 335 VPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGEIDSLYKEGL------ 394

Query: 292 HEILQFNIISTLNASNQPAPPSVQPTPVQPDTPVIKFPPPEALKRDKFNWLSDSEFARQT 351
             +L+      +    +   P +  T  +    ++++  P+ + +DK+ WL D EFARQ 
Sbjct: 395 --LLKLGFQDDM--FKKFPLPKIVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQA 454

Query: 352 IAGLNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIG--SSITVEEAIAQKKLF 411
           IAG+NP  I+ V S+P  S LDP  YGP   +  TE    +IG    +TV++A+   +LF
Sbjct: 455 IAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTE--DHIIGQLDGLTVQQALETNRLF 514

Query: 412 VLDYHDTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRPPMAGKPQ-WKQV 464
           ++DYHD  + +++++ ++     Y +RT+ FL    TL P+ IEL+ P  +   Q  K+V
Sbjct: 515 MVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSSNQKSKRV 559

BLAST of CSPI04G13440 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 240.4 bits (612), Expect = 3.0e-63
Identity = 173/460 (37.61%), Postives = 242/460 (52.61%), Query Frame = 0

Query: 10  SVIPKINETFPSDDTLLLFFASSDL-DRDGQQKPLIGGVAEL-----IKNESTAEKYKYV 69
           S + +++E   +  TL L   SSD+ D +   K  +G  A L          TA +  + 
Sbjct: 41  SFLDRLHEFLGNKITLRL--VSSDVTDSENGSKGKLGKAAHLEDWITTITSLTAGESAFK 100

Query: 70  AKIQVPKGFGEIGAVIVELKDDSSEKFIDTVIVANPTSHNTITFSCTSWVQP-KSLIPDQ 129
                   FG  GA ++      SE  + ++ + +   H  + + C SW+ P K    D 
Sbjct: 101 VTFDYETDFGYPGAFLIR-NSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTD- 160

Query: 130 RRVFFSTKSYLPGDTPAGLLKLRAEDLENLRGQKEDGTVDTNERKAFERIYDYDVYNDLG 189
            RVFFS K+YLP +TPA LLK R E+L +LRG  E       E K ++R+YDY  YNDLG
Sbjct: 161 -RVFFSNKTYLPHETPATLLKYREEELVSLRGTGE------GELKEWDRVYDYAYYNDLG 220

Query: 190 DPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR----STESFYVPRDEAFSE 249
            P    K  RPVLGG+ +YPYPRR RTGR  T  DP++E R    S+   YVPRDE F  
Sbjct: 221 VP---PKNPRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGH 280

Query: 250 VKQKMFPSNPGK------KDKLGA------KPFTDFTQIDLMFRDGIAPPSNPHEILQFN 309
           +K   F +   K      +  L A      K F  F  +  ++ +GI     P++ L  +
Sbjct: 281 LKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIYEEGI---DLPNQALIDS 340

Query: 310 IISTLNASNQPAPPSVQPTPVQPD-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNP 369
           I+  +       P  +     + D    +KFP P+ +K DK  W +D EFAR+ +AGLNP
Sbjct: 341 IVKNI-------PLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNP 400

Query: 370 YCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTL 429
             IQL+K +P KS+LD   YG Q S  T   ++  +   +TVEEA+ +++LF+LD+HDTL
Sbjct: 401 VVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNL-DGLTVEEALEKERLFILDHHDTL 460

Query: 430 MQYVEKVRSIKRTTLYGSRTLFFLNDDDTLTPLGIELTRP 446
           M Y+ +V +   T  Y SRTL FL DD TL PL IEL+ P
Sbjct: 461 MPYLGRVNT-TTTKTYASRTLLFLKDDGTLKPLVIELSLP 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
R9WS041.7e-9845.58Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=... [more]
P384194.8e-9844.47Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX... [more]
Q8GSM23.1e-9745.81Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1[more]
Q84YK83.4e-9644.40Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O243701.7e-9543.89Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... [more]
Match NameE-valueIdentityDescription
A0A5D3DDX52.4e-24993.09Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00120 P... [more]
A0A5A7TH322.7e-24589.58Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00110 P... [more]
A0A5A7TJ252.5e-24390.50Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00140 P... [more]
A0A6J1J6545.5e-21481.47Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483812 PE=3 SV=1[more]
A0A6J1FUE71.6e-21382.11Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448228 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142240.31.5e-26699.78linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KAE8649493.1 hy... [more]
TYK21650.14.9e-24993.09linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa][more]
KAA0041366.15.6e-24589.58linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa][more]
KAA0041369.15.2e-24390.50linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa][more]
XP_011653576.24.6e-23990.71linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KAE8649492.1 hy... [more]
Match NameE-valueIdentityDescription
AT3G45140.11.6e-9344.50lipoxygenase 2 [more]
AT1G17420.19.4e-7338.59lipoxygenase 3 [more]
AT1G67560.11.0e-6638.51PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G72520.12.2e-6636.07PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G55020.13.0e-6337.61lipoxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 314..330
score: 39.17
coord: 353..372
score: 46.91
coord: 97..115
score: 34.94
coord: 202..221
score: 69.15
coord: 169..185
score: 78.47
coord: 421..445
score: 59.91
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 3..129
e-value: 9.3E-7
score: 38.4
IPR001024PLAT/LH2 domainPFAMPF01477PLATcoord: 56..127
e-value: 3.8E-4
score: 21.0
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 5..127
score: 13.571579
IPR027433Lipoxygenase, domain 3GENE3D4.10.372.10coord: 251..345
e-value: 1.4E-17
score: 66.1
NoneNo IPR availableGENE3D3.10.450.60coord: 346..469
e-value: 3.4E-38
score: 132.7
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 3..150
e-value: 2.6E-27
score: 97.5
NoneNo IPR availableGENE3D4.10.375.10coord: 152..250
e-value: 4.4E-34
score: 118.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 454..469
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 449..469
NoneNo IPR availablePANTHERPTHR11771:SF156LIPOXYGENASEcoord: 22..463
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 141..466
e-value: 9.4E-101
score: 338.3
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 131..469
score: 90.150482
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 22..463
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 133..463
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 25..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G13440.1CSPI04G13440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity