CSPI04G13220 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G13220
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic
Location	Chr4: 11332132 .. 11335039 (-)
RNA-Seq Expression	CSPI04G13220
Synteny	CSPI04G13220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAGTATCTAATAGCTTCTCATCAGCATAAACCCTACACCCTGTGAAGTGAAGAAGAGAGGAAAAATGTTGATTGCTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTTTGCGTTTCATTTCACACTTGTCTTCTTCACCTATCTGCTCAAACCCATCAATCCTTTTCCCACTTCCTTCCACTCTTTCCCGTTCTTTCTCTTCCAAACCCACTTCTCCACAAGCTTTGAGTCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGTTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCATCAAACCCTCTAAGAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTTCCCGTCGATGATGAATCCGAATGCTCTGAATTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGAACGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTGAGGCACTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCTGATTTTGATTTCAATCATACTCTCGGTGGGAGATTGGTGTCTACTACGGGAACCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCAAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGAATAATTCAGCCGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAACAGATTTGCTGCCTAGTTCTTTATCACCAGAAAAATTCGAGCACTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATAAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCAAGAGGAAATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGGAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTAGAGGGAATTTTTCCTGCTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTGAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATCAGCAAGGAATTGAAACCCAGGACTTACAGGATTAAGGTAATGAAGTTGATCTTTTTTATGATCCTTTCTCATGCCTGAAACTTGATTCTGCTTCATTACCATTGAACCCTCGTTCTTTAAGTTTTAGTTTCAGAGAAGGTTGGGTCTTGGGATGGATAGGCAGTAGGCTATAATTTTCAGTCAGGTTATGTCCATCCCACTAATCTACTAATCTCACTAGATAACCTGCCTAATCCTACCAAGAAAGTTCAAGAAATTTCTAGGGTATCAAATGGATCGAATTTGTGACCTCATGTGCTCCTATTGACTAAATTAAAAAAATATGGTTTAGGAACCATATCGAAAATCTAAGTGAATATCAAGTTTTGTCCATATCGAAAATCTAAGTGAATATCAAGTTTTGTTATGTTGTGAAATTGCGATTGGCTAAATAGCAAAATGGTATAATACTATATAATGATTTTGAAATTGTGAGCGTTAAGCTTCTACTTCCAATTTGATCCTCTATAGACAAATATTTTCAGCAAGAAATGTGGTTAGGAATTCATAACTTTGGTGTTGCATTTTATAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATTTGGATACAATTTATGTTACAGTGTGGGCATCTCCTTATCTTCCACTACACATGGGGAAAACAGAAAATGCAACCAAAATGCAAGAGGACCATTTTGGTATTCAATTGCAGGTATTGTTTGCACTAATTAATTTGATGATGAACATACAGGATGGTCATTTTGTGGATAATCATTGTTTCTTGCTTTGCTTCAGCCACCAATAGGCAAAGATCGAGTAGAAGAACTTGGAAAATGGGTGAGAAGGGAGTTTCGTGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGTTGCTGGCCTGGGTTGGTTCGCCATTGGCCTAAAAGGCAAAGTAGTCTTAAGCATATGGACTTACGAGGGGGTCGATATTGTTTTACGAAGTTCTGTCATTCCTCATCGAGCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCCAAGGCTGATCAGGCTTCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCGAGGTTCATGTGCTTGCTCAAACGTAAGCATTGAGAGCTCACTTCGACTATGATTTTGCTTAAATGTTGTAGCATTGAACAGTAAGTGATCTATATCAATGTACAAGAATGGGGTTTGTCGGAATTTATGCTGGAGAATAGCAATGCAAGCAACTTCAAGCCTTTCAGCCCGTGATAAGTGTCCGGGATTCGTGATATATGAAGCCACATTCACGCTCGAAACTCGAACTAGGTTGGCATCATATCAGAGAGTTGTATACTTAAATCTACTGCTTCATTTTCGAGGAAGGCTTTAGCTTCTATATTCACTACGGGAGGCAGTGCAAAATGGAAGTAATTATAAACTTGAGGAATTGATTGTTTCCTCTACGGCTTTGCAGCCCCAAAATGTCGAGAGTTGTGGTTTTTTCTTTTTTTTCTTTTTCTTTTTTTGTAATCAAGTTGCCCATGTCTGATTAACATCCATCAAGATTAGTTCTTAGTCTTTCGATTTTTTCTATTCATTTGCAATAACCAGACTTCCATATTTATAATATAGGTTGAAATTGATA

mRNA sequence

ACAGTATCTAATAGCTTCTCATCAGCATAAACCCTACACCCTGTGAAGTGAAGAAGAGAGGAAAAATGTTGATTGCTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTTTGCGTTTCATTTCACACTTGTCTTCTTCACCTATCTGCTCAAACCCATCAATCCTTTTCCCACTTCCTTCCACTCTTTCCCGTTCTTTCTCTTCCAAACCCACTTCTCCACAAGCTTTGAGTCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGTTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCATCAAACCCTCTAAGAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTTCCCGTCGATGATGAATCCGAATGCTCTGAATTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGAACGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTGAGGCACTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCTGATTTTGATTTCAATCATACTCTCGGTGGGAGATTGGTGTCTACTACGGGAACCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCAAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGAATAATTCAGCCGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAACAGATTTGCTGCCTAGTTCTTTATCACCAGAAAAATTCGAGCACTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATAAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCAAGAGGAAATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGGAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTAGAGGGAATTTTTCCTGCTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTGAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATCAGCAAGGAATTGAAACCCAGGACTTACAGGATTAAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATTTGGATACAATTTATGTTACAGTGTGGGCATCTCCTTATCTTCCACTACACATGGGGAAAACAGAAAATGCAACCAAAATGCAAGAGGACCATTTTGGTATTCAATTGCAGCCACCAATAGGCAAAGATCGAGTAGAAGAACTTGGAAAATGGGTGAGAAGGGAGTTTCGTGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGTTGCTGGCCTGGGTTGGTTCGCCATTGGCCTAAAAGGCAAAGTAGTCTTAAGCATATGGACTTACGAGGGGGTCGATATTGTTTTACGAAGTTCTGTCATTCCTCATCGAGCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCCAAGGCTGATCAGGCTTCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCGAGGTTCATGTGCTTGCTCAAACGTAAGCATTGAGAGCTCACTTCGACTATGATTTTGCTTAAATGTTGTAGCATTGAACAGTAAGTGATCTATATCAATGTACAAGAATGGGGTTTGTCGGAATTTATGCTGGAGAATAGCAATGCAAGCAACTTCAAGCCTTTCAGCCCGTGATAAGTGTCCGGGATTCGTGATATATGAAGCCACATTCACGCTCGAAACTCGAACTAGGTTGGCATCATATCAGAGAGTTGTATACTTAAATCTACTGCTTCATTTTCGAGGAAGGCTTTAGCTTCTATATTCACTACGGGAGGCAGTGCAAAATGGAAGTAATTATAAACTTGAGGAATTGATTGTTTCCTCTACGGCTTTGCAGCCCCAAAATGTCGAGAGTTGTGGTTTTTTCTTTTTTTTCTTTTTCTTTTTTTGTAATCAAGTTGCCCATGTCTGATTAACATCCATCAAGATTAGTTCTTAGTCTTTCGATTTTTTCTATTCATTTGCAATAACCAGACTTCCATATTTATAATATAGGTTGAAATTGATA

Coding sequence (CDS)

ATGTTGATTGCTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTTTGCGTTTCATTTCACACTTGTCTTCTTCACCTATCTGCTCAAACCCATCAATCCTTTTCCCACTTCCTTCCACTCTTTCCCGTTCTTTCTCTTCCAAACCCACTTCTCCACAAGCTTTGAGTCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGTTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCATCAAACCCTCTAAGAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTTCCCGTCGATGATGAATCCGAATGCTCTGAATTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGAACGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTGAGGCACTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCTGATTTTGATTTCAATCATACTCTCGGTGGGAGATTGGTGTCTACTACGGGAACCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCAAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGAATAATTCAGCCGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAACAGATTTGCTGCCTAGTTCTTTATCACCAGAAAAATTCGAGCACTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATAAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCAAGAGGAAATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGGAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTAGAGGGAATTTTTCCTGCTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTGAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTTCACATCAGCAAGGAATTGAAACCCAGGACTTACAGGATTAAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATTTGGATACAATTTATGTTACAGTGTGGGCATCTCCTTATCTTCCACTACACATGGGGAAAACAGAAAATGCAACCAAAATGCAAGAGGACCATTTTGGTATTCAATTGCAGCCACCAATAGGCAAAGATCGAGTAGAAGAACTTGGAAAATGGGTGAGAAGGGAGTTTCGTGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGTTGCTGGCCTGGGTTGGTTCGCCATTGGCCTAAAAGGCAAAGTAGTCTTAAGCATATGGACTTACGAGGGGGTCGATATTGTTTTACGAAGTTCTGTCATTCCTCATCGAGCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCCAAGGCTGATCAGGCTTCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCGAGGTTCATGTGCTTGCTCAAACGTAA

Protein sequence

MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECSEFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKADQASNKPLGQSEKKKKGDPEVHVLAQT*

Homology

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BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot
Match: Q8W4I6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPG2 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.6e-125
Identity = 231/573 (40.31%), Postives = 340/573 (59.34%), Query Frame = 0


Query: 66  DETTSRFSLVCPGCGVHMQDSNPKHPGFFIK----------------------------- 125
           DE      ++CPGCG+ MQD++P  PG++ K                             
Sbjct: 88  DEDEDYGKIICPGCGIFMQDNDPDLPGYYQKRKVIANNLEGDEHVENDELAGFEMVDDDA 147

Query: 126 -------------------------------------PSKKDSNYRLLTHLVPV----DD 185
                                                  KK+ N   L    P      +
Sbjct: 148 DEEEEGEDDEMDDEIKNAIEGSNSESESGFEWESDEWEEKKEVNDVELDGFAPAGVGYGN 207

Query: 186 ESECSEFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 245
            +E  E  KR    E +K   EE   +     TVC+RCHSLR+YG+VK+ + ENLLPDFD
Sbjct: 208 VTEEKEKKKRVSKTERKKIAREEAKKDNYDDVTVCARCHSLRNYGQVKNQAAENLLPDFD 267

Query: 246 FNHTLGGRLVS--TTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGN 305
           F+  +  RL+   +  + +VV++VVD  DFDGSFPK+ A  +   ++      K  K  N
Sbjct: 268 FDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGSFPKRAAKSLFQVLQKAENDPKGSK--N 327

Query: 306 VPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDV 365
           +P++VLV TK DLLP+ +SP + + WVR RA+ GG  K++ ++MVS+ +D G+KNL+  +
Sbjct: 328 LPKLVLVATKVDLLPTQISPARLDRWVRHRAKAGGAPKLSGVYMVSARKDIGVKNLLAYI 387

Query: 366 IELAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQ 425
            ELAG RGNVW IGAQNAGKSTLIN++ K  G K+T+LTEAPVPGTTLGI+++ GI  A+
Sbjct: 388 KELAGPRGNVWVIGAQNAGKSTLINALSKKDGAKVTRLTEAPVPGTTLGILKIGGILSAK 447

Query: 426 AKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETN 485
           AK++DTPGLL+P+ ++ RL  EE+K+V I KE++PR+YR+K G ++H+ GL+RLD+   +
Sbjct: 448 AKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQPRSYRVKAGQSVHIGGLVRLDLVSAS 507

Query: 486 LDTIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCG 545
           ++TIY+T+WAS  + LH+GKTENA ++ + H G++LQPPIG++R  ELG W  +E +V G
Sbjct: 508 VETIYITIWASHSVSLHLGKTENAEEIFKGHSGLRLQPPIGENRASELGTWEEKEIQVSG 567

Query: 546 TSWDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVS 567
            SWD   +D +VAGLGW ++GLKG   L++WTY+G+D+ LR  ++  RA + E  GF + 
Sbjct: 568 NSWDVKSIDISVAGLGWLSLGLKGAATLALWTYQGIDVTLREPLVIDRAPYLERPGFWLP 627

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BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot
Match: P54453 (Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.0e-41
Identity = 130/402 (32.34%), Postives = 199/402 (49.50%), Query Frame = 0


Query: 124 KRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTLGGR 183
           K GL   P    T+E+V        +C RC  L++Y +++D S    L D DF + L G 
Sbjct: 18  KTGLGYAPPASLTKENV--------ICQRCFRLKNYNEIQDVS----LTDDDFLNILHG- 77

Query: 184 LVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTK 243
                 T S+V+ +VD  DF+GS+   +  LV                GN   ++LV  K
Sbjct: 78  ---IGETDSLVVKIVDIFDFNGSWINGLQRLV---------------GGN--PILLVGNK 137

Query: 244 TDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNV 303
            D+LP SL  E+   W+++ A+E G+ K   + +VS+ R  GI+ ++ D IE      +V
Sbjct: 138 ADILPKSLKRERLIQWMKREAKELGL-KPVDVFLVSAGRGQGIREVI-DAIEHYRNGKDV 197

Query: 304 WAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLL 363
           + +G  N GKST IN I K V G+   +T +  PGTTL  I +       + L+DTPG++
Sbjct: 198 YVVGCTNVGKSTFINRIIKEVSGEEDIITTSQFPGTTLDAIEIP--LDDGSSLYDTPGII 257

Query: 364 NPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVTVWA 423
           N HQ+   + +++ K++   KELKPRT+++    T++  GL R D         YV+   
Sbjct: 258 NNHQMAHYVNKKDLKILSPKKELKPRTFQLNDQQTLYFGGLARFD---------YVSGER 317

Query: 424 SPY-------LPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSW 483
           SP+       L +H  K ENA  + E H G  L PP GKD ++E  + V   F +     
Sbjct: 318 SPFICYMPNELMIHRTKLENADALYEKHAGELLTPP-GKDEMDEFPELVAHTFTI----- 366

Query: 484 DSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVI 519
                D   +GLGW  +    K V + +  +GV + +R S+I
Sbjct: 378 KDKKTDIVFSGLGWVTVHDADKKV-TAYAPKGVHVFVRRSLI 366

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BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot
Match: Q66GP9 (NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NOA1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 3.8e-25
Identity = 95/372 (25.54%), Postives = 164/372 (44.09%), Query Frame = 0


Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     L  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TK DLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R +V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F    KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNLDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEE-LGKWVRREFRVCGTSWDSSCV 494
               L +H   T+ AT   E   G+ L PP GK++++E  G    R  ++          
Sbjct: 444 GPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

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BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot
Match: Q6YPG5 (Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0104700 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.1e-24
Identity = 93/366 (25.41%), Postives = 165/366 (45.08%), Query Frame = 0


Query: 146 KPTVCSRCHSLRHYGKVKDPSVENLLP---DFDFNHTLGGRLVSTTGTRSVVLIVVDAAD 205
           +  +C RC  L H   +         P    F     L  +L      +++++ +VD  D
Sbjct: 133 RTVLCGRCKLLSHGHMITAVGGHGGYPGGKQFVSADQLRDKLSYLRHEKALIIKLVDIVD 192

Query: 206 FDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQ 265
           F+GSF  +V +   A+                  ++LV+TK DLLP          WV +
Sbjct: 193 FNGSFLARVRDFAGAN-----------------PIILVITKVDLLPRDTDLNCIGDWVVE 252

Query: 266 RAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGK 325
              +  +N + S+H+ SS    G+  ++ ++ +    R +V+ +G+ N GKS  I+++ +
Sbjct: 253 AVVKKKLN-VLSVHLTSSKSLVGVTGVISEIQQEKKGR-DVYILGSANVGKSAFISAMLR 312

Query: 326 HVGGKIT--------QLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTR 385
            +  K          +  ++ VPGTTLG I++E  F    KL+DTPG+   H+    +  
Sbjct: 313 TMAYKDPVAAAAQKYKPIQSAVPGTTLGPIQIEA-FLGGGKLYDTPGVHLHHRQAAVIHA 372

Query: 386 EEQKLVHISKELKPRTYRIK------VGHTIHVAGLMRLDVEETNLDTIYVTVWASPYLP 445
           ++   +     L+ R +          G+++   GL+R+DV +  L    +T +    L 
Sbjct: 373 DDLPSLAPQSRLRARCFPANDTDVGLSGNSLFWGGLVRIDVVKA-LPRTRLTFYGPKKLK 432

Query: 446 LHMGKTENATKMQEDHFGIQLQPPIGKDRVEE-LGKWVRREFRVCGTSWDSSCVDAAVAG 494
           ++M  T  A +  E   G+ L PP GK++ E  +G    RE ++     D    D A++G
Sbjct: 433 INMVPTTEADEFYEREVGVTLTPPAGKEKAEGWVGLQGVRELQIKYEESDRPACDIAISG 477

[Close]

BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot
Match: Q9JJG9 (Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.1e-11
Identity = 48/171 (28.07%), Postives = 82/171 (47.95%), Query Frame = 0


Query: 357 FDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDT 416
           +DTPG+     I   LT +E   V  +  + PRT+ +K G  + + G+ R+D  + N  +
Sbjct: 496 YDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPGMVLFLGGIARIDFLQGN-QS 555

Query: 417 IYVTVWASPYLPLHMGKTENATKMQEDHFG--IQLQPPIGKDRVEELGKWVRREFRVCGT 476
            + TV AS +LP+H+   + A  + E H G  + L P  GK+R+ +    V  +  + G 
Sbjct: 556 AWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAEDITLKGG 615

Query: 477 SWDSSCVDAAVAGLGWFAIG--LKGKVVLSIWTYEGVDIVLRSSVIPHRAN 524
               +  D   +  GW A+    +G + L   T EG  + +   V+P+  N
Sbjct: 616 GKFEAVADIKFSSAGWVAVTPYSEGTLHLRGHTPEGTALTVHPPVLPYIVN 665

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BLAST of CSPI04G13220 vs. ExPASy TrEMBL
Match: A0A0A0KZ58 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 562/566 (99.29%), Postives = 563/566 (99.47%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFL LRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQA SLS
Sbjct: 1   MLIARKLSASNLKSFLFLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQAFSLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAAVAGLGWFAIGLKG+VVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAVAGLGWFAIGLKGEVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QASNKPLGQSEKKKK DPEVHVLAQT
Sbjct: 541 QASNKPLGQSEKKKKSDPEVHVLAQT 566

[Close]

BLAST of CSPI04G13220 vs. ExPASy TrEMBL
Match: A0A1S3BNH1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491566 PE=4 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.1e-307
Identity = 528/566 (93.29%), Postives = 545/566 (96.29%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QA+NK LGQSEKKKKGDP+VH+LA+T
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

[Close]

BLAST of CSPI04G13220 vs. ExPASy TrEMBL
Match: A0A5D3DDC5 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00340 PE=4 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 1.9e-306
Identity = 527/566 (93.11%), Postives = 545/566 (96.29%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPV+DESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVNDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QA+NK LGQSEKKKKGDP+VH+LA+T
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

[Close]

BLAST of CSPI04G13220 vs. ExPASy TrEMBL
Match: A0A5A7TAR0 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00150 PE=4 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.5e-303
Identity = 523/559 (93.56%), Postives = 538/559 (96.24%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPE 560
           QA+NK LGQSEKKKKGDP+
Sbjct: 541 QAANKQLGQSEKKKKGDPK 559

[Close]

BLAST of CSPI04G13220 vs. ExPASy TrEMBL
Match: A0A6J1FW06 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448990 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 480/581 (82.62%), Postives = 527/581 (90.71%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFI-----SHLSSSPICSNPSILFPL---------PSTLSRS 60
           MLIARKLSA  LK  LSLRF+     S L SSP CSNP IL PL         PS+ S+ 
Sbjct: 1   MLIARKLSALKLKPILSLRFVSPLQRSQLFSSPSCSNPRILCPLPSATPLPLSPSSSSQP 60

Query: 61  FSSKPTSPQALSLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRL 120
           FSSKPT P++  L+RDGN+ E TSRFSLVCPGCGVHMQD+N KHPGFF+KPS+KDSNYRL
Sbjct: 61  FSSKPTPPESPLLTRDGNYSEATSRFSLVCPGCGVHMQDTNRKHPGFFVKPSRKDSNYRL 120

Query: 121 LTHLVPVDDESECSEFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPS 180
           LTHLVPVDDESECS+ LKRGLV+EPE +K+EE+VNEK QKPTVCSRCHSLRHYGKVKDP+
Sbjct: 121 LTHLVPVDDESECSDLLKRGLVMEPEHQKSEENVNEKAQKPTVCSRCHSLRHYGKVKDPA 180

Query: 181 VENLLPDFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAW 240
           +ENLLPDFDF+HT+G RLVSTTGTRSVVL+VVDAADFDGSFPKKVANLVSA+IE+NS  W
Sbjct: 181 LENLLPDFDFDHTIGRRLVSTTGTRSVVLLVVDAADFDGSFPKKVANLVSATIEDNSTVW 240

Query: 241 KQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGI 300
           KQGKSGNVPRVVLVVTK DLLPSS+SP +FEHWVRQRAREGGINKITSLHMVS++RDWG+
Sbjct: 241 KQGKSGNVPRVVLVVTKIDLLPSSVSPTRFEHWVRQRAREGGINKITSLHMVSAVRDWGL 300

Query: 301 KNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV 360
           K+LVEDVI+L G RGNVWA+GAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV
Sbjct: 301 KSLVEDVIDLVGPRGNVWAVGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV 360

Query: 361 EGIFPAQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMR 420
           EG+FP QAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIK GHTIHVAGLMR
Sbjct: 361 EGMFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKAGHTIHVAGLMR 420

Query: 421 LDVEETNLDTIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVR 480
           LDVEET++DTIYVTVWASPYLPLHMGKTENA+K+ EDHFG QLQPPIGKDRV ELGKWVR
Sbjct: 421 LDVEETSVDTIYVTVWASPYLPLHMGKTENASKILEDHFGNQLQPPIGKDRVAELGKWVR 480

Query: 481 REFRVCGTSWDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFE 540
           REF VCG+SWDSSC+DAA AGLGWFAIGLKG+ VLSIWTYEG+++VLRSSVIP R+NFFE
Sbjct: 481 REFHVCGSSWDSSCMDAAAAGLGWFAIGLKGEAVLSIWTYEGIEVVLRSSVIPDRSNFFE 540

Query: 541 DAGFTVSKIVSKADQASNKPLGQSEKK-KKGDPEVHVLAQT 567
           DAGFTVSK+VSKADQA+NK L QSE+K KKGD +VHVLA+T
Sbjct: 541 DAGFTVSKVVSKADQAANKSLDQSERKTKKGDSKVHVLAET 581

[Close]

BLAST of CSPI04G13220 vs. NCBI nr
Match: XP_004142247.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Cucumis sativus] >KGN54094.1 hypothetical protein Csa_018182 [Cucumis sativus])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 562/566 (99.29%), Postives = 563/566 (99.47%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFL LRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQA SLS
Sbjct: 1   MLIARKLSASNLKSFLFLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQAFSLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAAVAGLGWFAIGLKG+VVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAVAGLGWFAIGLKGEVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QASNKPLGQSEKKKK DPEVHVLAQT
Sbjct: 541 QASNKPLGQSEKKKKSDPEVHVLAQT 566

[Close]

BLAST of CSPI04G13220 vs. NCBI nr
Match: XP_008449781.1 (PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.1e-306
Identity = 528/566 (93.29%), Postives = 545/566 (96.29%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QA+NK LGQSEKKKKGDP+VH+LA+T
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

[Close]

BLAST of CSPI04G13220 vs. NCBI nr
Match: TYK21671.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1060.8 bits (2742), Expect = 4.0e-306
Identity = 527/566 (93.11%), Postives = 545/566 (96.29%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPV+DESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVNDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPEVHVLAQT 567
           QA+NK LGQSEKKKKGDP+VH+LA+T
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

[Close]

BLAST of CSPI04G13220 vs. NCBI nr
Match: KAA0040048.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.2e-303
Identity = 523/559 (93.56%), Postives = 538/559 (96.24%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQALSLS 60
           MLIARKLSASNLKSFLS RFI HLSSSP CSNPSILFPLPSTL  SFSSKPT PQAL+LS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFFIKPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180
           EFLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HTL
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240
           GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300
           VTKTDLLPSSLSP  FE WVRQRAREGGINKITSLHMVS+I+DWGIKNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360
           GNVWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK++EDHFGIQLQPPIGKDRV ELGKWVR+EFRVCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWF+IGLKG+ VLSIWTYEGV+IVLRSSVIP RANFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 QASNKPLGQSEKKKKGDPE 560
           QA+NK LGQSEKKKKGDP+
Sbjct: 541 QAANKQLGQSEKKKKGDPK 559

[Close]

BLAST of CSPI04G13220 vs. NCBI nr
Match: XP_038901740.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.4e-292
Identity = 496/571 (86.87%), Postives = 537/571 (94.05%), Query Frame = 0


Query: 1   MLIARKLSASNLKSFLSLRFI-----SHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQ 60
           MLI RKLSASNLKSFLSLR +     S L SSP+C NP ILFP PSTL RSFSSKPT P+
Sbjct: 1   MLITRKLSASNLKSFLSLRSVSLSLSSQLFSSPLCLNPPILFPHPSTLPRSFSSKPTPPE 60

Query: 61  ALSLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDD 120
           A +L+RDGN+DE TSRFSLVCPGCGVHMQD+NPKHPGFF+KPS+KD NYRLLTHLVPVDD
Sbjct: 61  ASNLTRDGNYDEATSRFSLVCPGCGVHMQDTNPKHPGFFVKPSRKDPNYRLLTHLVPVDD 120

Query: 121 ESECSEFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 180
           ESECSEFLKRGLVI+PE +KTEE+V E+PQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD
Sbjct: 121 ESECSEFLKRGLVIDPENQKTEENVIEQPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 180

Query: 181 FNHTLGGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVP 240
           F+HT+G RLVSTTGTRSVV+IVVDA DFDGSFPKKVANLVSA+IE+NSAAWKQGKSGNVP
Sbjct: 181 FDHTIGRRLVSTTGTRSVVMIVVDATDFDGSFPKKVANLVSATIEDNSAAWKQGKSGNVP 240

Query: 241 RVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIE 300
           RVVLVVTK DLLPSS+SP +FEHWVRQRAREGGINKITSLHMVS++RDWG+KNLVEDVI+
Sbjct: 241 RVVLVVTKIDLLPSSISPTRFEHWVRQRAREGGINKITSLHMVSAVRDWGLKNLVEDVID 300

Query: 301 LAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAK 360
           L GARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIR+EGIFP QA+
Sbjct: 301 LVGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRMEGIFPGQAR 360

Query: 361 LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLD 420
           LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEET++D
Sbjct: 361 LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETSVD 420

Query: 421 TIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTS 480
           TIYVTVWASPYLPLHMGKTENA+K+Q DHFG QLQPPIGKDRV ELGKWVRREFRVCG+S
Sbjct: 421 TIYVTVWASPYLPLHMGKTENASKIQ-DHFGNQLQPPIGKDRVAELGKWVRREFRVCGSS 480

Query: 481 WDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKI 540
           WDSSCVDAA AGLGWFAIGLKG+ VLSIWTYEGV++V+RSSVIP+R+NFFEDAGFT+SKI
Sbjct: 481 WDSSCVDAAAAGLGWFAIGLKGEAVLSIWTYEGVEVVIRSSVIPYRSNFFEDAGFTISKI 540

Query: 541 VSKADQASNKPLGQSEKKKKGDPEVHVLAQT 567
           VSKADQ + KPL Q EKKKKGDP+VHVLA+T
Sbjct: 541 VSKADQVATKPLDQREKKKKGDPKVHVLAET 570

[Close]

BLAST of CSPI04G13220 vs. TAIR 10
Match: AT4G10620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.2e-210
Identity = 363/570 (63.68%), Postives = 444/570 (77.89%), Query Frame = 0


Query: 4   ARKLSASNLKSFLSLRFISHLSSSPICSNPSILFPLPSTLS------------RSFSSKP 63
           AR+LS+S LK   +L   S  SS P   NPS     PS L+              F S  
Sbjct: 6   ARELSSSKLKPLFALHLSSFKSSIPTKPNPS----PPSYLNPHHFNNISKPPFLRFYSSS 65

Query: 64  TSPQALSLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPS-KKDSNYRLLTHL 123
           +S   L L+RDGN+++TTS    VCPGCGVHMQ+SNPKHPGFFIKPS +K  N   L  L
Sbjct: 66  SSSNLLPLNRDGNYNDTTSITISVCPGCGVHMQNSNPKHPGFFIKPSTEKQRNDLNLRDL 125

Query: 124 VPVDDESECSEFLKRGLVIEP--EKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVE 183
            P+  E E  + +KRG +IEP    +    D      +P VC+RCHSLRHYG+VKDP+VE
Sbjct: 126 TPISQEPEFIDSIKRGFIIEPISSSDLNPRDDEPSDSRPLVCARCHSLRHYGRVKDPTVE 185

Query: 184 NLLPDFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQ 243
           NLLPDFDF+HT+G RL S +G R+VVL+VVDA+DFDGSFPK+VA LVS +I+ N+ AWK+
Sbjct: 186 NLLPDFDFDHTVGRRLGSASGARTVVLMVVDASDFDGSFPKRVAKLVSRTIDENNMAWKE 245

Query: 244 GKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKN 303
           GKSGNVPRVV+VVTK DLLPSSLSP +FE WVR RAREGG++KIT LH VS +++WGIK+
Sbjct: 246 GKSGNVPRVVVVVTKIDLLPSSLSPNRFEQWVRLRAREGGLSKITKLHFVSPVKNWGIKD 305

Query: 304 LVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEG 363
           LVEDV  +AG RG+VWA+G+QNAGKSTLIN++GK VGGK+  LTEAPVPGTTLGIIR+EG
Sbjct: 306 LVEDVAAMAGKRGHVWAVGSQNAGKSTLINAVGKVVGGKVWHLTEAPVPGTTLGIIRIEG 365

Query: 364 IFPAQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLD 423
           + P +AKLFDTPGLLNPHQITTRLTREEQ+LVHISKELKPRTYRIK G+T+H+ GLMRLD
Sbjct: 366 VLPFEAKLFDTPGLLNPHQITTRLTREEQRLVHISKELKPRTYRIKEGYTVHIGGLMRLD 425

Query: 424 VEETNLDTIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRRE 483
           ++E ++D++YVTVWASPY+PLHMGK ENA K  EDHFG +LQPPIG+ RVEELGKWVR+E
Sbjct: 426 IDEASVDSLYVTVWASPYVPLHMGKKENAYKTLEDHFGCRLQPPIGEKRVEELGKWVRKE 485

Query: 484 FRVCGTSWDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDA 543
           FRV GTSWD+S VD AV+GLGWFA+GLKG  +L +WT+EG+D+  R S++P RA+ FED+
Sbjct: 486 FRVSGTSWDTSSVDIAVSGLGWFALGLKGDAILGVWTHEGIDVFCRDSLLPQRAHTFEDS 545

Query: 544 GFTVSKIVSKADQASNKPLGQSEKKKKGDP 559
           GFTVSKIV+KAD+  N+ + + E +KK  P
Sbjct: 546 GFTVSKIVAKADRNFNQ-IHKEETQKKRKP 570

[Close]

BLAST of CSPI04G13220 vs. TAIR 10
Match: AT3G57180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 4.0e-126
Identity = 231/573 (40.31%), Postives = 340/573 (59.34%), Query Frame = 0


Query: 66  DETTSRFSLVCPGCGVHMQDSNPKHPGFFIK----------------------------- 125
           DE      ++CPGCG+ MQD++P  PG++ K                             
Sbjct: 88  DEDEDYGKIICPGCGIFMQDNDPDLPGYYQKRKVIANNLEGDEHVENDELAGFEMVDDDA 147

Query: 126 -------------------------------------PSKKDSNYRLLTHLVPV----DD 185
                                                  KK+ N   L    P      +
Sbjct: 148 DEEEEGEDDEMDDEIKNAIEGSNSESESGFEWESDEWEEKKEVNDVELDGFAPAGVGYGN 207

Query: 186 ESECSEFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 245
            +E  E  KR    E +K   EE   +     TVC+RCHSLR+YG+VK+ + ENLLPDFD
Sbjct: 208 VTEEKEKKKRVSKTERKKIAREEAKKDNYDDVTVCARCHSLRNYGQVKNQAAENLLPDFD 267

Query: 246 FNHTLGGRLVS--TTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGN 305
           F+  +  RL+   +  + +VV++VVD  DFDGSFPK+ A  +   ++      K  K  N
Sbjct: 268 FDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGSFPKRAAKSLFQVLQKAENDPKGSK--N 327

Query: 306 VPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDV 365
           +P++VLV TK DLLP+ +SP + + WVR RA+ GG  K++ ++MVS+ +D G+KNL+  +
Sbjct: 328 LPKLVLVATKVDLLPTQISPARLDRWVRHRAKAGGAPKLSGVYMVSARKDIGVKNLLAYI 387

Query: 366 IELAGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQ 425
            ELAG RGNVW IGAQNAGKSTLIN++ K  G K+T+LTEAPVPGTTLGI+++ GI  A+
Sbjct: 388 KELAGPRGNVWVIGAQNAGKSTLINALSKKDGAKVTRLTEAPVPGTTLGILKIGGILSAK 447

Query: 426 AKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETN 485
           AK++DTPGLL+P+ ++ RL  EE+K+V I KE++PR+YR+K G ++H+ GL+RLD+   +
Sbjct: 448 AKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQPRSYRVKAGQSVHIGGLVRLDLVSAS 507

Query: 486 LDTIYVTVWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCG 545
           ++TIY+T+WAS  + LH+GKTENA ++ + H G++LQPPIG++R  ELG W  +E +V G
Sbjct: 508 VETIYITIWASHSVSLHLGKTENAEEIFKGHSGLRLQPPIGENRASELGTWEEKEIQVSG 567

Query: 546 TSWDSSCVDAAVAGLGWFAIGLKGKVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVS 567
            SWD   +D +VAGLGW ++GLKG   L++WTY+G+D+ LR  ++  RA + E  GF + 
Sbjct: 568 NSWDVKSIDISVAGLGWLSLGLKGAATLALWTYQGIDVTLREPLVIDRAPYLERPGFWLP 627

[Close]

BLAST of CSPI04G13220 vs. TAIR 10
Match: AT3G47450.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-26
Identity = 95/372 (25.54%), Postives = 164/372 (44.09%), Query Frame = 0


Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     L  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TK DLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R +V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F    KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNLDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEE-LGKWVRREFRVCGTSWDSSCV 494
               L +H   T+ AT   E   G+ L PP GK++++E  G    R  ++          
Sbjct: 444 GPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

[Close]

BLAST of CSPI04G13220 vs. TAIR 10
Match: AT3G47450.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 4.7e-26
Identity = 95/372 (25.54%), Postives = 164/372 (44.09%), Query Frame = 0


Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFNHTLGGRLVSTTGTRSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     L  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLVVTKTDLLPSSLSPEKFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TK DLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGIKNLVEDVIELAGARGNVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R +V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F    KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNLDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEE-LGKWVRREFRVCGTSWDSSCV 494
               L +H   T+ AT   E   G+ L PP GK++++E  G    R  ++          
Sbjct: 444 GPKALEIHAVPTKTATAFYEAKLGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

The following BLAST results are available for this feature:

BLAST of CSPI04G13220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W4I6	5.6e-125	40.31	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arab...	[more]
P54453	1.0e-41	32.34	Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH...	[more]
Q66GP9	3.8e-25	25.54	NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]
Q6YPG5	1.1e-24	25.41	Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g...	[more]
Q9JJG9	1.1e-11	28.07	Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1	[more]

BLAST of CSPI04G13220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ58	0.0e+00	99.29	G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1	[more]
A0A1S3BNH1	5.1e-307	93.29	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu...	[more]
A0A5D3DDC5	1.9e-306	93.11	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m...	[more]
A0A5A7TAR0	1.5e-303	93.56	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m...	[more]
A0A6J1FW06	1.6e-279	82.62	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu...	[more]

BLAST of CSPI04G13220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142247.1	0.0e+00	99.29	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform...	[more]
XP_008449781.1	1.1e-306	93.29	PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplas...	[more]
TYK21671.1	4.0e-306	93.11	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. mak...	[more]
KAA0040048.1	3.2e-303	93.56	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. mak...	[more]
XP_038901740.1	7.4e-292	86.87	GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Beninc...	[more]

BLAST of CSPI04G13220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10620.1	1.2e-210	63.68	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT3G57180.1	4.0e-126	40.31	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT3G47450.2	2.7e-26	25.54	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT3G47450.1	4.7e-26	25.54	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 303..378 e-value: 7.4E-9 score: 35.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 183..367 e-value: 5.2E-34 score: 119.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 191..380
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 542..566
None	No IPR available	PANTHER	PTHR46434	GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL	coord: 22..546
None	No IPR available	CDD	cd01855	YqeH	coord: 150..362 e-value: 7.66336E-61 score: 197.872

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G13220.1	CSPI04G13220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004517	nitric-oxide synthase activity