CSPI04G12670 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G12670
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	NADH-cytochrome b5 reductase
Location	Chr4: 11005947 .. 11010772 (+)
RNA-Seq Expression	CSPI04G12670
Synteny	CSPI04G12670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAATTTCATATAAATGTTAATCCCATCAGAAGAAGATTCGAAGTCAGTTCAGTAATGGGAGTGGTAGTGGGTTGTAAAAAGCGTATTATGATTTGATTTATGCGGAAAGTAAAAAAAAATCCATTGATTCATTCATTCCGTATTTCCCTTCATCCGCATCTCTCGAGAACCATTGAAGCCTTGTGATCCGATTCCTCGCTTTTCCTCCCCAAATGCCGACGAGTTACAGGCCTGAGCTTCTCACCGTCGCCGTCGCTGTGGTTGCTGTCGGCGTTGCCACTGCCTGCTATTTCTACGTCGCCAAGAAATCCAAACCCAAAGGTTCTTGTTCCTTCTACTCGTGCCTTTTTTTGTTGTTTTGCTTGATGAGGATAGGATTCCGGGCTCCGAGTATTGGGATTTGAGAATACCTTGAACAAACCCTTCCCAACCCTGTTTCATTTTATTGTAATTTGCGATTTCTTGCTACTTCAAGATGTGTTCGATGTCTTTTTTTTTACTTGTTGTTTTCTGTTATGATAGGTTGCTTGGACCCTGATAACTTCAAGGAATTTAAACTAGTTAAACGCACACAACTTAGCCATAATGTAGCAAAGTTCAGATTCTCTCTTCCGACTCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGCTGCAGGTTTTATTGACTTTAATACTTGTTTGATTCAAGAAGAAACTCTCTCCACTTTGTATAGTGGAGTGAAGATTCATCTATGGAATGTTATGAATCTTCAAACGATTTGTTGATGTGTTGTTGTACAGAGGAAAAGATAGCCAAAGTGAAGATGTTGTGAAACCTTACACTCCAACCACTTTGGACATAGATGTTGGATACTTTGAGTTGGTCATTAAGGTATACCCTTAAACTTCCAATATGCAATAGTTTGCAGTTTTTCTTGTTAATTAACCATTTGCTCTCTTCTATCTATGACATTTGGTATTTTGGCTTACAACTACGATTGTATGTAGATGTATCCACAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTACTTGGCTGTGAAAGGTCCAAAGGTATTGCTAACTTGTCCTTCAACAGCAACTACATTAAGTAGAATTTGTTAATTTGAACATTGAATATAAGGCTTCAATAAGTAATATAGTTAATACCTTTCATTAGTCTACTTTTCTTCTCTTTTTTTTCCCAATATCTGATTAGTCGCGAGTATTTACACATATGGCTGGATTGGGTGTGGATGTGTTTGTGGGTGCTAGTTTCTGTGGTGGTATTGGATGCTTTTTAGATGTGTTTAAGTATTCTATTGCAGAGTTGATGAGTTGTTTTCTTTTGTTTTAATATTTTGGTTCATACAAGTTTTTGGCTCGATTCTAGTCGTTTATTTGTTGAGTTTTTAGTTGATTTATTGATTGGTATGTGAGTAATGTGTATTAGTTATTCATTTCATCTAAGGAGGTATTGATTTAACCACTTATCAAATCTTTAGAGTTTGAATTGGATGGAATTAAAAATTGAAGGCAAAGACTGACCATCTAGGATAAAAATAACCTCTTTTTGGGAGATTCATCGTTCAATATTAGGTTATTAGAACTTCGAGGAGTGCATTGAACAGTCAACTAGTTTTGGTCCCAAATTTTTAGTTCTCGAGGTGCGAGGTGCTCTTAAATATTGATTTCTTTACCTTTGATTTGCAAAAATCTAAATACTGGGTCAGGGAATCTTGGATTTGGGCCATTTGGCATAATATTAGTAATGCCATAAGGTTATATGGTCTCTGGAAGAAGGTTCAATGGGGGTATTCCGAGTGCAGGGTTGTGAGGCAGGGGAGTTTCACAAATTTGAACAACAGTGACTTGAACTACCATATTAAACCCACAATTTCATCCAAATGTTAAGTTACTGGTTAAAGGCAAATTTAATTATATATCACTAACAATCTTATTTGCATTCAACTATATTTCTTTATTTAGTACACAAATTTACATTCAAGTCCAGTCATGACAAGAGACTGGTGCAGTTAGTTCCGATGAAAGAGTGCATTGAAGTTCATTTTTCCTTGATTAATTCTATTATCCAAGTAACTAACAATATTTTATCTTGATAACCGTTTGCTTATATGGCCCCTACAGCCCTACCCAACTTGAAAATTTATAGAGAATAAAGCACTTAATTAGCCTTTGCACACACTGTCGCACTCGAACCATATTATTTATTATTTATTATTTATGGAGTTCTTTTATGTCTGTGTGTGTAAATGGTGGCAGGGACGATTCAAATATGAACCTAATGAAGTAGAGGCCTTTGGAATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTATGAGCATGGGCTAAGTTTTGAGTGAAGATTCGTATTGGTATTTGTGTTCTATCTGTGTGTCTTAACAAACAAATATATTCAACATTGCTGAACGTTTTCTTTTTCAGGTTGCAAGAGCAATACTAGAAAATGAAGTGGATCGCACCAACGTGCATCTTATCTATGCTAATGTTACTTACGAAGACATTCTTCTGAAGGTGCTCTTTCTGGTCTCTTAATTTGGATTTGGAAATTGAAACTACCCTACAATAAACCACGTATACCCTCTATTCTACTTGTGTTTGCAGGAAGAATTGGATAGTCTTGCAACTACGTTCCCTACCCGTTTTTCTATCTACTATGTGCTCAATCAGGTAAATAACTTGGGGGTTTTACAAATACATTCTGCAGTTTCACTATGTCTTTTTGTAATTGCTCTCTGCACTGTACAGCCTCCAGAAGAATGGGATGGTGGTGTTGGCTTTGTAACTGAGGAAATGATACGACTACACTGTCCTGCGCCATCCCCTAATGTCCGGGTGTGTACTCTGCTTTGCTTTACTACACTCTTGACACTTCATTCGCTTTCCATTCTTTCTGTAAGAGAGAGTTTGTGCTAAAAGAAGGCAATGTTCTTCATTTTGCAACAGATTTTGAGGTGCGGACCACCCCCTATGAACAAAGCTATGGCTGCTTACCTGAACAGTATTGGCTACACTTCTCAAATGCAGTTCCAGTTCTAATAATCCAAAGTTTCCAATCCCATTATTTACTTTTTTTACTTTACTTACCCCATTAAATTCAATTCATCCAATCTCGTGAAATTAAAATGTATATCCATCCAAACGAAGAATCACATTAATAAAATGGTGGACTATATCAATGTTTGGAATAAAACTTGATTTGATGATTTCACACAAAAACAAAGCTAAGGCAGACTACAACTACAAACAAAGCTAGAGGAAAGATGCAACCTTGTGAGTCTCTGTTCTCCCAACAATTCCACACTTGATGAGACTTCCAATGGTGTTCCACATCAACTTAGCATCTTCCAATCTGTTTCCCTCAGCCAATTTCTTGTACAGATAGCTTTCAAATGTCATTCTCTGCACATACTCATTCCCACACATTTCCACCAATGCCTCCCTTCCGGCATTGTCCCCATAGAACACTCTCTGCAACACATCCAAGAACCTGAACGTTCCCACGTACTTGGCATCCCACTCTCTCAAATACTCTCTCTTCAGATCCCTTTCTCCGATCATCCTCTCCCCTCCTTCCGATGCTCTCACCACCGCCTCTCCGCACATTCTCCCCGATTTCGCTGCAAAATATATGCCTTCACCCGAGCACTTTGTCACGTATCCTGCCGCGTCACCCACCAGCGCCACCCGCCCTACCACTCGCCTTGCTCGCGGGTGCTCCGGTATCGGATGTGCCTCTACTTTCATCACCTTCCCTCCGCTTATCTTCCTCTTTGCCCTTTCCCTTATTCCTCTTTGGTAACGCTTTATGTCTTGTTTCGAGCACACCGTCCCCGTCCCCACTGCCACATGGTCGCATTTTGGAAATACCCAAGCATAAAAATCTGGCGATACGTCGTTCCCTACGTACACTTCCGCCAGATTCTCGTAGTACTCCATTTTCTCCTCTGGCAATTTGATTCTCTCTTGGAAGGCGATCGCACACGTGTAATCTCCAGCGTCGATCGACTTCGCTACACGGCTGTTGGCGCCGTCCGCACCGATCACCACATCCACGGCCAAGGATTTCCGGGAGTTTTCAGCTGTGTAATGCACGACGTACGGTTCTTTGGAGGAGGTTGGGAGCTCGAGATTCAGCACGAGGCCATGGATGAGATTAGCACCGTTGGACTGAGCGCGGGAACGGAGGAAGGAATCGAGCACTTCGCGGCGTAGCATGGGTATGAACTCATGGGCTTTCAAGGTTTTTCCGAAGTCGACGGCGAGATTGGAAGGGGAAATAAATTTCATACGAGTAACATGGCGATCGATGAGATGAGCAGGGATGTCGAACTCATTTAGCATACATAGAGGGAGAGCGCCGCCACAGGGCTTGGCAGAGGATGGACTGCGCTCGAATAGGTAAGTCTCGATGCCACCAGCTGCGAGAGCTTCGGCAGCGGAAGATCCCGCAGGGCCACCGCCAATGACGGCAGCTCGGAGCTTGCGGCCAATAAGAGGGGCATTGTCGGATTTAGAGGAGGAGGTTATGAGAAGAGAAGGAGGGCGGCGGGAGGATGGAGTGTGTTTGGTGGTAGGGTTTAGAGAAAGAGAGGAGAAACCCTGCAGGCAACGCGCTGCCATTGAAGGAAGTTAAGATCTGGAAATGAAGGCGGAATGATTTAGGTTTCTGCCGTCTAGTCTTTTTTGAGAAACCTTTGTTTAAAGGGATGTTGAGTGCTTCCCATTACCAGTTTGGCAGTACGAAAAGGACATTGGATTTGGTTTATTTTAT

mRNA sequence

AACAATTTCATATAAATGTTAATCCCATCAGAAGAAGATTCGAAGTCAGTTCAGTAATGGGAGTGGTAGTGGGTTGTAAAAAGCGTATTATGATTTGATTTATGCGGAAAGTAAAAAAAAATCCATTGATTCATTCATTCCGTATTTCCCTTCATCCGCATCTCTCGAGAACCATTGAAGCCTTGTGATCCGATTCCTCGCTTTTCCTCCCCAAATGCCGACGAGTTACAGGCCTGAGCTTCTCACCGTCGCCGTCGCTGTGGTTGCTGTCGGCGTTGCCACTGCCTGCTATTTCTACGTCGCCAAGAAATCCAAACCCAAAGGTTGCTTGGACCCTGATAACTTCAAGGAATTTAAACTAGTTAAACGCACACAACTTAGCCATAATGTAGCAAAGTTCAGATTCTCTCTTCCGACTCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGCTGCAGAGGAAAAGATAGCCAAAGTGAAGATGTTGTGAAACCTTACACTCCAACCACTTTGGACATAGATGTTGGATACTTTGAGTTGGTCATTAAGATGTATCCACAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTACTTGGCTGTGAAAGGTCCAAAGGGACGATTCAAATATGAACCTAATGAAGTAGAGGCCTTTGGAATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTTGCAAGAGCAATACTAGAAAATGAAGTGGATCGCACCAACGTGCATCTTATCTATGCTAATGTTACTTACGAAGACATTCTTCTGAAGGAAGAATTGGATAGTCTTGCAACTACGTTCCCTACCCGTTTTTCTATCTACTATGTGCTCAATCAGCCTCCAGAAGAATGGGATGGTGGTGTTGGCTTTGTAACTGAGGAAATGATACGACTACACTGTCCTGCGCCATCCCCTAATGTCCGGATTTTGAGGTGCGGACCACCCCCTATGAACAAAGCTATGGCTGCTTACCTGAACAGTATTGGCTACACTTCTCAAATGCAGTTCCAGTTCTAATAATCCAAAGTTTCCAATCCCATTATTTACTTTTTTTACTTTACTTACCCCATTAAATTCAATTCATCCAATCTCGTGAAATTAAAATGTATATCCATCCAAACGAAGAATCACATTAATAAAATGGTGGACTATATCAATGTTTGGAATAAAACTTGATTTGATGATTTCACACAAAAACAAAGCTAAGGCAGACTACAACTACAAACAAAGCTAGAGGAAAGATGCAACCTTGTGAGTCTCTGTTCTCCCAACAATTCCACACTTGATGAGACTTCCAATGGTGTTCCACATCAACTTAGCATCTTCCAATCTGTTTCCCTCAGCCAATTTCTTGTACAGATAGCTTTCAAATGTCATTCTCTGCACATACTCATTCCCACACATTTCCACCAATGCCTCCCTTCCGGCATTGTCCCCATAGAACACTCTCTGCAACACATCCAAGAACCTGAACGTTCCCACGTACTTGGCATCCCACTCTCTCAAATACTCTCTCTTCAGATCCCTTTCTCCGATCATCCTCTCCCCTCCTTCCGATGCTCTCACCACCGCCTCTCCGCACATTCTCCCCGATTTCGCTGCAAAATATATGCCTTCACCCGAGCACTTTGTCACGTATCCTGCCGCGTCACCCACCAGCGCCACCCGCCCTACCACTCGCCTTGCTCGCGGGTGCTCCGGTATCGGATGTGCCTCTACTTTCATCACCTTCCCTCCGCTTATCTTCCTCTTTGCCCTTTCCCTTATTCCTCTTTGGTAACGCTTTATGTCTTGTTTCGAGCACACCGTCCCCGTCCCCACTGCCACATGGTCGCATTTTGGAAATACCCAAGCATAAAAATCTGGCGATACGTCGTTCCCTACGTACACTTCCGCCAGATTCTCGTAGTACTCCATTTTCTCCTCTGGCAATTTGATTCTCTCTTGGAAGGCGATCGCACACGTGTAATCTCCAGCGTCGATCGACTTCGCTACACGGCTGTTGGCGCCGTCCGCACCGATCACCACATCCACGGCCAAGGATTTCCGGGAGTTTTCAGCTGTGTAATGCACGACGTACGGTTCTTTGGAGGAGGTTGGGAGCTCGAGATTCAGCACGAGGCCATGGATGAGATTAGCACCGTTGGACTGAGCGCGGGAACGGAGGAAGGAATCGAGCACTTCGCGGCGTAGCATGGGTATGAACTCATGGGCTTTCAAGGTTTTTCCGAAGTCGACGGCGAGATTGGAAGGGGAAATAAATTTCATACGAGTAACATGGCGATCGATGAGATGAGCAGGGATGTCGAACTCATTTAGCATACATAGAGGGAGAGCGCCGCCACAGGGCTTGGCAGAGGATGGACTGCGCTCGAATAGGTAAGTCTCGATGCCACCAGCTGCGAGAGCTTCGGCAGCGGAAGATCCCGCAGGGCCACCGCCAATGACGGCAGCTCGGAGCTTGCGGCCAATAAGAGGGGCATTGTCGGATTTAGAGGAGGAGGTTATGAGAAGAGAAGGAGGGCGGCGGGAGGATGGAGTGTGTTTGGTGGTAGGGTTTAGAGAAAGAGAGGAGAAACCCTGCAGGCAACGCGCTGCCATTGAAGGAAGTTAAGATCTGGAAATGAAGGCGGAATGATTTAGGTTTCTGCCGTCTAGTCTTTTTTGAGAAACCTTTGTTTAAAGGGATGTTGAGTGCTTCCCATTACCAGTTTGGCAGTACGAAAAGGACATTGGATTTGGTTTATTTTAT

Coding sequence (CDS)

ATGCCGACGAGTTACAGGCCTGAGCTTCTCACCGTCGCCGTCGCTGTGGTTGCTGTCGGCGTTGCCACTGCCTGCTATTTCTACGTCGCCAAGAAATCCAAACCCAAAGGTTGCTTGGACCCTGATAACTTCAAGGAATTTAAACTAGTTAAACGCACACAACTTAGCCATAATGTAGCAAAGTTCAGATTCTCTCTTCCGACTCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGCTGCAGAGGAAAAGATAGCCAAAGTGAAGATGTTGTGAAACCTTACACTCCAACCACTTTGGACATAGATGTTGGATACTTTGAGTTGGTCATTAAGATGTATCCACAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTACTTGGCTGTGAAAGGTCCAAAGGGACGATTCAAATATGAACCTAATGAAGTAGAGGCCTTTGGAATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTTGCAAGAGCAATACTAGAAAATGAAGTGGATCGCACCAACGTGCATCTTATCTATGCTAATGTTACTTACGAAGACATTCTTCTGAAGGAAGAATTGGATAGTCTTGCAACTACGTTCCCTACCCGTTTTTCTATCTACTATGTGCTCAATCAGCCTCCAGAAGAATGGGATGGTGGTGTTGGCTTTGTAACTGAGGAAATGATACGACTACACTGTCCTGCGCCATCCCCTAATGTCCGGATTTTGAGGTGCGGACCACCCCCTATGAACAAAGCTATGGCTGCTTACCTGAACAGTATTGGCTACACTTCTCAAATGCAGTTCCAGTTCTAA

Protein sequence

MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF*

Homology

[Close]

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 1.9e-118
Identity = 206/273 (75.46%), Postives = 233/273 (85.35%), Query Frame = 0


Query: 6   RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 65
           R  LL V VA VAVG A A YF  + K K + CLDP+NFKEFKLVKR QLSHNVAKF F 
Sbjct: 11  RQILLGVFVAFVAVG-AGAAYFLTSSK-KRRVCLDPENFKEFKLVKRHQLSHNVAKFVFE 70

Query: 66  LPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHHF 125
           LPT TS+LGLPIGQH+SCRGKD Q EDV+KPYTPTTLD DVG FELVIKMYPQGRMSHHF
Sbjct: 71  LPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQGRMSHHF 130

Query: 126 REMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLI 185
           REMR GD+LAVKGPKGRFKY+P +  AFGMLAGG+GITPMFQVARAILEN  D+T VHLI
Sbjct: 131 REMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVHLI 190

Query: 186 YANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSP 245
           YANVTY+DILLKEEL+ L T +P +F I+YVLNQPPE WDGGVGFV++EMI+ HCPAP+ 
Sbjct: 191 YANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAPAS 250

Query: 246 NVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           +++ILRCGPPPMNKAMAA L ++GY+ +MQFQF
Sbjct: 251 DIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 4.8e-69
Identity = 133/272 (48.90%), Postives = 177/272 (65.07%), Query Frame = 0


Query: 6   RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 65
           + E +  AVA+ A+      +      SKP+  L+P  F+EF L ++T +SHNV  +RF+
Sbjct: 27  KKEFVPYAVALTAILAGLKLF---TGGSKPRKVLNPTEFQEFVLKEKTDISHNVCIYRFA 86

Query: 66  LPTPTSILGLPIGQHMSCRGK-DSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHH 125
           LP P  ILGLPIGQH+S     + Q ++VV+ YTP + D + GYF+L++K YPQG +S +
Sbjct: 87  LPRPADILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKY 146

Query: 126 FREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEV-----DR 185
              ++ GD + V+GPKG   Y PN     GM+AGGTGITPM Q+ +A++ N       D 
Sbjct: 147 LTTLKIGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDT 206

Query: 186 TNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLH 245
           T + LI+ANV  +DILLKEELD LA   P  F+IYYVLN PP+ W GGVGFVT EMI+ H
Sbjct: 207 TKLDLIFANVNPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEH 266

Query: 246 CPAPSPNVRILRCGPPPMNKAMAAYLNSIGYT 272
            PAP+ +V+IL CGPPPM  AM     S+GYT
Sbjct: 267 LPAPASDVKILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot
Match: A1C7E9 (NADH-cytochrome b5 reductase 1 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=cbr1 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 8.1e-69
Identity = 136/272 (50.00%), Postives = 177/272 (65.07%), Query Frame = 0


Query: 6   RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 65
           + E +  AVA+ AV    A +      SK +  L+P  F+EF L ++T +SHNV+ +RF+
Sbjct: 27  KKEFVPYAVALTAV---LAGFKLFTGDSKARKVLNPTEFQEFVLKEKTDISHNVSIYRFA 86

Query: 66  LPTPTSILGLPIGQHMSCRGK-DSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHH 125
           LP PT ILGLPIGQH+S     + Q ++VV+ YTP + D + GYF+L++K YPQG +S H
Sbjct: 87  LPRPTDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKH 146

Query: 126 FREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENE-----VDR 185
              ++ GD + V+GPKG   Y PN     GM+AGGTGITPM QV +AI+ N       D 
Sbjct: 147 LTTLKVGDVMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQVIKAIIRNRPRNGGTDI 206

Query: 186 TNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLH 245
           T V LI+ANV  EDILLKEELD LA      F+IYYVLN PP+ W GGVGFVT EMI+  
Sbjct: 207 TKVDLIFANVNPEDILLKEELDKLAAE-DEDFNIYYVLNNPPQGWTGGVGFVTPEMIKER 266

Query: 246 CPAPSPNVRILRCGPPPMNKAMAAYLNSIGYT 272
            PAP+ +V++L CGPPPM  AM     S+G+T
Sbjct: 267 LPAPASDVKVLLCGPPPMISAMKKATESLGFT 294

[Close]

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-68
Identity = 133/272 (48.90%), Postives = 177/272 (65.07%), Query Frame = 0


Query: 6   RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 65
           + E +  AVAV A+      +      +KP+  L+P  F+EF L ++  +SHNV  +RF+
Sbjct: 27  KKEFVPYAVAVTAILAGLKLF---TGGNKPRKVLNPTEFQEFVLKEKIDVSHNVCIYRFA 86

Query: 66  LPTPTSILGLPIGQHMSCRGK-DSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHH 125
           LP PT ILGLPIGQH+S     + Q ++VV+ YTP + D + GYF+L++K YPQG +S +
Sbjct: 87  LPRPTDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKY 146

Query: 126 FREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEV-----DR 185
              ++ GD + V+GPKG   Y PN     GM+AGGTGITPM Q+ +A++ N       D 
Sbjct: 147 LTTLKIGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDT 206

Query: 186 TNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLH 245
           T + LI+ANV  +DILLKEELD LA   P  F+IYYVLN PP+ W GGVGFVT EMI+ H
Sbjct: 207 TKLDLIFANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEH 266

Query: 246 CPAPSPNVRILRCGPPPMNKAMAAYLNSIGYT 272
            PAP+ +V+IL CGPPPM  AM     S+GYT
Sbjct: 267 LPAPASDVKILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot
Match: Q6BUX2 (NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.8e-68
Identity = 125/264 (47.35%), Postives = 172/264 (65.15%), Query Frame = 0


Query: 7   PELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFSL 66
           P ++   VA +        +F +++K+ P   L P++F++F L+++T++SHN   +RF L
Sbjct: 8   PFIIFATVAAIISSAVAYYFFQLSRKNAP--VLKPNDFQKFPLIEKTRVSHNTCVYRFGL 67

Query: 67  PTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHHFR 126
           P  T  LGLPIGQH++  G     ++VV+ YTP + D ++GYF+L+IK Y  G +S H  
Sbjct: 68  PRSTDRLGLPIGQHIAI-GATINDKEVVRSYTPISTDDELGYFDLLIKAYENGNISRHVE 127

Query: 127 EMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLIY 186
             + G+ + ++GPKG F Y P  VE+FGM+AGGTGITPM+Q+  AIL N  D+T V L+Y
Sbjct: 128 SKKIGETIDIRGPKGFFTYTPGMVESFGMIAGGTGITPMYQILTAILRNPEDKTKVSLVY 187

Query: 187 ANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSPN 246
           ANVT +DILLKEEL+ +A   P RF IYYVLN PPE W GGVGFVT E++  H P  S  
Sbjct: 188 ANVTEDDILLKEELNKMAREHPDRFKIYYVLNTPPENWTGGVGFVTPEIMDKHLPKASEA 247

Query: 247 VRILRCGPPPMNKAMAAYLNSIGY 271
             +L CGPPPM  AM      +GY
Sbjct: 248 TNLLLCGPPPMISAMKKAAVGLGY 268

[Close]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL
Match: A0A0A0KZ12 (NADH-cytochrome b5 reductase OS=Cucumis sativus OX=3659 GN=Csa_4G269170 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.6e-160
Identity = 277/278 (99.64%), Postives = 277/278 (99.64%), Query Frame = 0


Query: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60
           MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA
Sbjct: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60

Query: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGR 120
           KFRFSLPTPTSILGLPIGQHMSCRGKDSQSED VKPYTPTTLDIDVGYFELVIKMYPQGR
Sbjct: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDAVKPYTPTTLDIDVGYFELVIKMYPQGR 120

Query: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180
           MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT
Sbjct: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180

Query: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240
           NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC
Sbjct: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240

Query: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF
Sbjct: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 278

[Close]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL
Match: A0A5D3DDX7 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00930 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.6e-155
Identity = 269/278 (96.76%), Postives = 272/278 (97.84%), Query Frame = 0


Query: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60
           MPTS+RPELLT+ VAVVAVGVATA YFYVAKKSK KGCLDPDNFKEFKLVKRTQLSHNVA
Sbjct: 1   MPTSFRPELLTITVAVVAVGVATAYYFYVAKKSKSKGCLDPDNFKEFKLVKRTQLSHNVA 60

Query: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGR 120
           KFRFSLPTPTSILGLPIGQHMSCRGKDSQ EDVVKPYTPTTLD DVGYFELVIKMYPQGR
Sbjct: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQGEDVVKPYTPTTLDTDVGYFELVIKMYPQGR 120

Query: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180
           MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILEN VDRT
Sbjct: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENPVDRT 180

Query: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240
           NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC
Sbjct: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240

Query: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           PAPSPNVRILRCGPPPMNKAMAAYLN+IGYTSQMQFQF
Sbjct: 241 PAPSPNVRILRCGPPPMNKAMAAYLNTIGYTSQMQFQF 278

[Close]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL
Match: A0A1S3BN07 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103491618 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.6e-155
Identity = 269/278 (96.76%), Postives = 272/278 (97.84%), Query Frame = 0


Query: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60
           MPTS+RPELLT+ VAVVAVGVATA YFYVAKKSK KGCLDPDNFKEFKLVKRTQLSHNVA
Sbjct: 1   MPTSFRPELLTITVAVVAVGVATAYYFYVAKKSKSKGCLDPDNFKEFKLVKRTQLSHNVA 60

Query: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGR 120
           KFRFSLPTPTSILGLPIGQHMSCRGKDSQ EDVVKPYTPTTLD DVGYFELVIKMYPQGR
Sbjct: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQGEDVVKPYTPTTLDTDVGYFELVIKMYPQGR 120

Query: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180
           MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILEN VDRT
Sbjct: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENPVDRT 180

Query: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240
           NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC
Sbjct: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240

Query: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           PAPSPNVRILRCGPPPMNKAMAAYLN+IGYTSQMQFQF
Sbjct: 241 PAPSPNVRILRCGPPPMNKAMAAYLNTIGYTSQMQFQF 278

[Close]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL
Match: A0A6J1FXX1 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.0e-143
Identity = 246/275 (89.45%), Postives = 260/275 (94.55%), Query Frame = 0


Query: 4   SYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFR 63
           S+RPE LTVAVA VA+GVATA YFY+ KKSKP+GCLDPDNFKEFKLVKR QLSHNVAKFR
Sbjct: 8   SHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLSHNVAKFR 67

Query: 64  FSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSH 123
           FSLPTPTSILGLPIGQHMSCRGKDS+ E+VVKPYTPT+LD DVGYF+LVIKMYPQGRMSH
Sbjct: 68  FSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSH 127

Query: 124 HFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVH 183
           HFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILEN VD TNVH
Sbjct: 128 HFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVH 187

Query: 184 LIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAP 243
           LIYAN TYEDILLKEELDSLA+ FP+RFS+YYVL+QPPE WDGGVGFVTEEMIRLHCP P
Sbjct: 188 LIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGP 247

Query: 244 SPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           SPNVRILRCGPPPMNKAMAAYL +IGYTSQMQFQF
Sbjct: 248 SPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

[Close]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL
Match: A0A6J1J953 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.3e-141
Identity = 242/275 (88.00%), Postives = 256/275 (93.09%), Query Frame = 0


Query: 4   SYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFR 63
           S+RPE LTVAVA VA+GVAT  YFY+ KKSKP+GCLDPD FKEFKLVKRTQLSHNVAKFR
Sbjct: 8   SHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLSHNVAKFR 67

Query: 64  FSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSH 123
           FSLPTPTSILGLPIGQHMSCRGKDS+ E+VVKPYTPT+LD DVGYF+LVIKMYPQGRMSH
Sbjct: 68  FSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSH 127

Query: 124 HFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVH 183
           HFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILEN VD TN H
Sbjct: 128 HFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNAH 187

Query: 184 LIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAP 243
           LIYAN TYEDILLKEELDSLA+ FP+RFS+YYVL+QPPE WDGGVGFVTEEMIRLHCP P
Sbjct: 188 LIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGP 247

Query: 244 SPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           SPNVRILRCGPPPMNKAM AYL +IGYT QMQFQF
Sbjct: 248 SPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 282

[Close]

BLAST of CSPI04G12670 vs. NCBI nr
Match: XP_004149649.1 (NADH--cytochrome b5 reductase 1 [Cucumis sativus] >KGN54034.1 hypothetical protein Csa_021640 [Cucumis sativus])

HSP 1 Score: 573.9 bits (1478), Expect = 7.3e-160
Identity = 277/278 (99.64%), Postives = 277/278 (99.64%), Query Frame = 0


Query: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60
           MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA
Sbjct: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60

Query: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGR 120
           KFRFSLPTPTSILGLPIGQHMSCRGKDSQSED VKPYTPTTLDIDVGYFELVIKMYPQGR
Sbjct: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDAVKPYTPTTLDIDVGYFELVIKMYPQGR 120

Query: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180
           MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT
Sbjct: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180

Query: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240
           NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC
Sbjct: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240

Query: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF
Sbjct: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 278

[Close]

BLAST of CSPI04G12670 vs. NCBI nr
Match: XP_008449872.1 (PREDICTED: NADH--cytochrome b5 reductase 1-like [Cucumis melo] >KAA0040098.1 NADH--cytochrome b5 reductase 1-like [Cucumis melo var. makuwa] >TYK21723.1 NADH--cytochrome b5 reductase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 557.8 bits (1436), Expect = 5.4e-155
Identity = 269/278 (96.76%), Postives = 272/278 (97.84%), Query Frame = 0


Query: 1   MPTSYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVA 60
           MPTS+RPELLT+ VAVVAVGVATA YFYVAKKSK KGCLDPDNFKEFKLVKRTQLSHNVA
Sbjct: 1   MPTSFRPELLTITVAVVAVGVATAYYFYVAKKSKSKGCLDPDNFKEFKLVKRTQLSHNVA 60

Query: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGR 120
           KFRFSLPTPTSILGLPIGQHMSCRGKDSQ EDVVKPYTPTTLD DVGYFELVIKMYPQGR
Sbjct: 61  KFRFSLPTPTSILGLPIGQHMSCRGKDSQGEDVVKPYTPTTLDTDVGYFELVIKMYPQGR 120

Query: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRT 180
           MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILEN VDRT
Sbjct: 121 MSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENPVDRT 180

Query: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240
           NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC
Sbjct: 181 NVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHC 240

Query: 241 PAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           PAPSPNVRILRCGPPPMNKAMAAYLN+IGYTSQMQFQF
Sbjct: 241 PAPSPNVRILRCGPPPMNKAMAAYLNTIGYTSQMQFQF 278

[Close]

BLAST of CSPI04G12670 vs. NCBI nr
Match: XP_038901049.1 (NADH--cytochrome b5 reductase 1-like [Benincasa hispida])

HSP 1 Score: 535.4 bits (1378), Expect = 2.9e-148
Identity = 258/282 (91.49%), Postives = 266/282 (94.33%), Query Frame = 0


Query: 1   MPTSY----RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLS 60
           MPTS+    RPELLT AVAVVA+GVATA YFY+ KKSKPK CLDPDNFKEFKL KRTQLS
Sbjct: 1   MPTSFVPSHRPELLTAAVAVVAIGVATAYYFYITKKSKPKACLDPDNFKEFKLAKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQH+SCRGKDS+ EDVVKPYTPTTLD DVGYF+LVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHISCRGKDSEGEDVVKPYTPTTLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENE 180
           PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILEN 
Sbjct: 121 PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDRTNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMI 240
           VDRTNVHLIYANVTYEDILLKEELDS AT FPTRF+IYYVLNQPPE WDGGVGFVTE+MI
Sbjct: 181 VDRTNVHLIYANVTYEDILLKEELDSFATRFPTRFTIYYVLNQPPEGWDGGVGFVTEQMI 240

Query: 241 RLHCPAPSPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           RLHCPAPSPNVRILRCGPPPMNKAMAAYL SIGYTSQMQFQF
Sbjct: 241 RLHCPAPSPNVRILRCGPPPMNKAMAAYLQSIGYTSQMQFQF 282

[Close]

BLAST of CSPI04G12670 vs. NCBI nr
Match: KAG6570317.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 246/275 (89.45%), Postives = 260/275 (94.55%), Query Frame = 0


Query: 4   SYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFR 63
           S+RPE LTVAVA VA+GVATA YFY+ KKSKP+GCLDPDNFKEFKLVKRTQLSHNVAKFR
Sbjct: 8   SHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLSHNVAKFR 67

Query: 64  FSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSH 123
           FSLPTPTSILGLPIGQHMSCRGKDS+ E+VVKPYTPT+LD DVGYF+LVIKMYPQGRMSH
Sbjct: 68  FSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSH 127

Query: 124 HFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVH 183
           HFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILEN VD TNVH
Sbjct: 128 HFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVH 187

Query: 184 LIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAP 243
           LIYAN TYEDILLKEELDSL + FP+RFS+YYVL+QPPE WDGGVGFVTEEMIRLHCP P
Sbjct: 188 LIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGP 247

Query: 244 SPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           SPNVRILRCGPPPMNKAMAAYL +IGYTSQMQFQF
Sbjct: 248 SPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

[Close]

BLAST of CSPI04G12670 vs. NCBI nr
Match: XP_022944293.1 (NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 519.2 bits (1336), Expect = 2.1e-143
Identity = 246/275 (89.45%), Postives = 260/275 (94.55%), Query Frame = 0


Query: 4   SYRPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFR 63
           S+RPE LTVAVA VA+GVATA YFY+ KKSKP+GCLDPDNFKEFKLVKR QLSHNVAKFR
Sbjct: 8   SHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLSHNVAKFR 67

Query: 64  FSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSH 123
           FSLPTPTSILGLPIGQHMSCRGKDS+ E+VVKPYTPT+LD DVGYF+LVIKMYPQGRMSH
Sbjct: 68  FSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSH 127

Query: 124 HFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVH 183
           HFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILEN VD TNVH
Sbjct: 128 HFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVH 187

Query: 184 LIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAP 243
           LIYAN TYEDILLKEELDSLA+ FP+RFS+YYVL+QPPE WDGGVGFVTEEMIRLHCP P
Sbjct: 188 LIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGP 247

Query: 244 SPNVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           SPNVRILRCGPPPMNKAMAAYL +IGYTSQMQFQF
Sbjct: 248 SPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

[Close]

BLAST of CSPI04G12670 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 426.8 bits (1096), Expect = 1.4e-119
Identity = 206/273 (75.46%), Postives = 233/273 (85.35%), Query Frame = 0


Query: 6   RPELLTVAVAVVAVGVATACYFYVAKKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 65
           R  LL V VA VAVG A A YF  + K K + CLDP+NFKEFKLVKR QLSHNVAKF F 
Sbjct: 11  RQILLGVFVAFVAVG-AGAAYFLTSSK-KRRVCLDPENFKEFKLVKRHQLSHNVAKFVFE 70

Query: 66  LPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHHF 125
           LPT TS+LGLPIGQH+SCRGKD Q EDV+KPYTPTTLD DVG FELVIKMYPQGRMSHHF
Sbjct: 71  LPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQGRMSHHF 130

Query: 126 REMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLI 185
           REMR GD+LAVKGPKGRFKY+P +  AFGMLAGG+GITPMFQVARAILEN  D+T VHLI
Sbjct: 131 REMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVHLI 190

Query: 186 YANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSP 245
           YANVTY+DILLKEEL+ L T +P +F I+YVLNQPPE WDGGVGFV++EMI+ HCPAP+ 
Sbjct: 191 YANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAPAS 250

Query: 246 NVRILRCGPPPMNKAMAAYLNSIGYTSQMQFQF 279
           +++ILRCGPPPMNKAMAA L ++GY+ +MQFQF
Sbjct: 251 DIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CSPI04G12670 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 209.9 bits (533), Expect = 2.6e-54
Identity = 107/266 (40.23%), Postives = 160/266 (60.15%), Query Frame = 0


Query: 31  KKSKPKGCLDPDNFKEFKLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCR-----G 90
           +++ PK  L+PD + EFKL    ++SHN   FRFS   P++ LGL +   +  R      
Sbjct: 65  EETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSF-DPSAELGLHVASCLLTRAPLGYN 124

Query: 91  KDSQSEDVVKPYTPTTLDIDVGYFELVIKMYPQGRMSHHFREMREGDYLAVKGPKGRFKY 150
            + +++ V++PYTP +     GYF+L+IK+YP G+MS HF  ++ GD L VKGP  +FKY
Sbjct: 125 AEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKY 184

Query: 151 EPNEVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLIYANVTYEDILLKEELDSLAT 210
            PN  +  GM+AGG+GITPM QV  AI++N  D T + L+YANV+ +DILLK++LD L  
Sbjct: 185 SPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLDVLQA 244

Query: 211 TFPTRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSPNVRILRCGPPPMNKAMA--- 270
             P    I+Y ++ P + W GGVG+++++M     P P+ +  IL CGPP M + ++   
Sbjct: 245 NHP-NLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHISGGK 304

Query: 271 ----------AYLNSIGYTSQMQFQF 279
                       L  +GYT +M F+F
Sbjct: 305 APDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of CSPI04G12670 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 169.9 bits (429), Expect = 3.0e-42
Identity = 91/230 (39.57%), Postives = 135/230 (58.70%), Query Frame = 0


Query: 48  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVG 107
           +LV++T +SH+V KFRF+LP    +LGLP+G+H+         +  ++ YTP++    VG
Sbjct: 666 QLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCA-TINDKLCLRAYTPSSTVDVVG 725

Query: 108 YFELVIKMY-----PQ----GRMSHHFREMREGDYLAVKGPKGRFKY----------EPN 167
           YFELV+K+Y     P+    G MS +   +  G  L +KGP G  +Y          +P 
Sbjct: 726 YFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHGKPK 785

Query: 168 EVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLIYANVTYEDILLKEELDSLATTFP 227
             +   MLAGGTGITP++Q+ +AIL++  D T +++IYAN T EDILL+EELD  A  +P
Sbjct: 786 FADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREELDGWAEQYP 845

Query: 228 TRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCP-APSPNVRILRCGPPPM 258
            R  ++YV+    E W    GF++E ++R H P     +   + CGPPPM
Sbjct: 846 DRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPM 894

[Close]

BLAST of CSPI04G12670 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 163.3 bits (412), Expect = 2.8e-40
Identity = 89/244 (36.48%), Postives = 140/244 (57.38%), Query Frame = 0


Query: 48  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQSEDVVKPYTPTTLDIDVG 107
           +L+++T +SH+V KFRF+LP+    LGLP+G+H+     +   +  ++ YTPT+    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCA-NINDKLCLRAYTPTSAIDAVG 725

Query: 108 YFELVIKMY-----PQ----GRMSHHFREMREGDYLAVKGPKGRFKY----------EPN 167
           + +LV+K+Y     P+    G MS H   +  G  + +KGP G  +Y          +P 
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 168 EVEAFGMLAGGTGITPMFQVARAILENEVDRTNVHLIYANVTYEDILLKEELDSLATTFP 227
             +   MLAGGTGITP++Q+ ++IL +  D T ++++YAN T +DIL++EEL+  A+   
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 228 TRFSIYYVLNQPPEEWDGGVGFVTEEMIRLHCPAPSPNVRI-LRCGPPPMNK-AMAAYLN 271
            R  I+YV+    E W    GF+TE ++R H P       + L CGPPPM + A+   L 
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

The following BLAST results are available for this feature:

BLAST of CSPI04G12670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	1.9e-118	75.46	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
A1DHW1	4.8e-69	48.90	NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]
A1C7E9	8.1e-69	50.00	NADH-cytochrome b5 reductase 1 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 5...	[more]
Q4X0B5	1.1e-68	48.90	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
Q6BUX2	1.8e-68	47.35	NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS...	[more]

BLAST of CSPI04G12670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ12	3.6e-160	99.64	NADH-cytochrome b5 reductase OS=Cucumis sativus OX=3659 GN=Csa_4G269170 PE=3 SV=...	[more]
A0A5D3DDX7	2.6e-155	96.76	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BN07	2.6e-155	96.76	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103491618 PE=3 SV=1	[more]
A0A6J1FXX1	1.0e-143	89.45	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 ...	[more]
A0A6J1J953	1.3e-141	88.00	NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=3 SV...	[more]

BLAST of CSPI04G12670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149649.1	7.3e-160	99.64	NADH--cytochrome b5 reductase 1 [Cucumis sativus] >KGN54034.1 hypothetical prote...	[more]
XP_008449872.1	5.4e-155	96.76	PREDICTED: NADH--cytochrome b5 reductase 1-like [Cucumis melo] >KAA0040098.1 NAD...	[more]
XP_038901049.1	2.9e-148	91.49	NADH--cytochrome b5 reductase 1-like [Benincasa hispida]	[more]
KAG6570317.1	1.3e-143	89.45	NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944293.1	2.1e-143	89.45	NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita moschata]	[more]

BLAST of CSPI04G12670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	1.4e-119	75.46	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	2.6e-54	40.23	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	3.0e-42	39.57	nitrate reductase 2	[more]
AT1G77760.1	2.8e-40	36.48	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 248..256 score: 37.29 coord: 154..173 score: 41.26 coord: 192..203 score: 44.16
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 154..173 score: 66.67 coord: 95..102 score: 44.79 coord: 192..203 score: 64.76 coord: 73..84 score: 53.47 coord: 248..256 score: 59.26 coord: 131..145 score: 48.19
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 136..278 e-value: 1.5E-41 score: 143.5
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 134..272
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 47..145 e-value: 2.7E-30 score: 104.7
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 34..135 e-value: 3.8E-33 score: 115.9
None	No IPR available	PANTHER	PTHR19370:SF204	NADH-CYTOCHROME B5 REDUCTASE	coord: 3..278
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 3..278
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..25 score: 5.0
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 47..278 e-value: 2.34973E-111 score: 319.127
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 155..261 e-value: 1.2E-28 score: 100.0
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 42..146 score: 13.848082
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 38..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G12670.1	CSPI04G12670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity