CSPI04G11360 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G11360
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	ribonuclease 3-like protein 2
Location	Chr4: 9622654 .. 9624560 (+)
RNA-Seq Expression	CSPI04G11360
Synteny	CSPI04G11360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTCTTCAAATTAACCTTCACTTCTCCTTTGTTCCTAAAAATCTTCTTTTTTTTTTCTTTTTTTTTTTTTTGCCCTGTTTTAACTTCATGTCTGTATTTTTTTTATTGTTCAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGGTAAGTTCTTAGTTACATTTGAAGTATGACTATTGTTACTTTATTAGCTGGGCACCAATTAGTTTTCTTTTCGTCTTCAATCTTTTGTAGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGGTATATATTTTTATTGTATGATATTTGAGATGTTGCATACTTGCATTGTTTTGGACTCTGAATTAGGAGGTAGTTTTTATGTCGTAGTTGTCTCTTTTTCATTTAAAAGCATGCACCTTCACTTATATATTTGCTTTCTAACCAAGTAGGATGCTGAATTTTTCAGTAATTTATAATGAAATGCATTTTTTTTCTCTAAAGTCTGCCTGCTTCAATTGTAACCTTGTATCTTCATCGAACTATTTTCATTCTTTCTGTTCATTGTAAAAGTTGCAGCATTTTTTAAATTTAAGATTCATTTCACTGATCAATGCTCACTTATTTTAGAGAAATTAGTTTCAGCAACGACAGTGACCTTCTCAATTAATTTTAATGTTATTATATCTGTACACTTATTTGTTTAAAACTTATTTTCTTTTGAATCTCCACCCTCTGTGCATACCTTATCAACTAAAGGCCTGTTTCCCTTCCTTTTCCATCTTACACATTGATTTTTTATGTGAATAGAACTCTTCGATATCTGTGGGGATTATCTCGAGTCGTTTTGTGCGGGTCATAAACAAAAGATAAAACTCAGAGAGATCATTTACATACCATGAAGTTATTTGACTATCTTTTTCCCTTCTCCTTGTCCTCCTTTTTCAAACGTGTTCTAGTGCTAAATACTTTATGAATTTCTTACTTGCAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA

mRNA sequence

ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA

Coding sequence (CDS)

ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA

Protein sequence

MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKIATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL*

Homology

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BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.5e-97
Identity = 188/333 (56.46%), Postives = 253/333 (75.98%), Query Frame = 0


Query: 4   SVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHL 63
           S+ A+E+IL+Y F+NKSLL+EA+TH S +   SYERLEF+GDSAIGLAI+NY +L  P L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR++P++D KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQ 183
            L+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D + +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI-A 303
                + ID      ++  AK KL+++C K+KW KP YSVE+D      + FVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           T   T Y+ GDE+S++K AE+S+A  MIRAL++
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRK 387

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BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-67
Identity = 142/277 (51.26%), Postives = 192/277 (69.31%), Query Frame = 0


Query: 8   LERILSYNFNNKSLLEEALTHPSYSASA-SYERLEFVGDSAIGLAITNYFFLASPHLHQG 67
           +ER+L Y F +  LLEEALTH S++  A SY+RLEFVGDSA+GLA +N+ +L +P L  G
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFADDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGPG 99

Query: 68  HLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEG----SSVPY- 127
            LS LRAAN+STEKLARVAV H LY  +RR+ P +D+ V +F+++V  E     S+VPY 
Sbjct: 100 PLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYG 159

Query: 128 GRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLF 187
           G ++KAPKVLADIVE++A AVYVD  FDL+KLW + R L EPI T E   ++ QPVT+L 
Sbjct: 160 GSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAE--TIDEQPVTMLH 219

Query: 188 QVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMD 247
           ++CQ++GK    K W+ G  ++ +V+V G+ V  GSS QK IA+LNAAR+A  KL+ +  
Sbjct: 220 ELCQKHGKMAQFKTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKLAGAKK 279

Query: 248 TRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKP 279
             + T V     DE  E+   KQKL++ CS++ W KP
Sbjct: 280 QVLTTGVGNGLGDEIGELRECKQKLNEQCSRQNWPKP 314

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BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot
Match: Q9FKF0 (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 138/384 (35.94%), Postives = 209/384 (54.43%), Query Frame = 0


Query: 3   SSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPH 62
           +SV A+E+IL+Y+F NK+LL+EA+T      S  ++RLEF GDS + +A TNY     P+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAIT----QKSPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYG 122
           L    L  LR ANVS EK AR+AV H L++++   +P++  KVK F ++V  E   VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQ-PVTVLF 182
            L+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT ++L ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 QVCQRNGKQ-----------------------VDIKHWKNGSNSIASVYVDGKFVASGSS 242
           ++  ++G +                       VD++  K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNAAREALIKLSDSMDTRIKTF---------------------------VTID 302
           +  E+++     +      +  D + K F                           + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVK--IATCYGTFYI 334
                 E    + +L ++C+K KW +P +SV+++ GP +E  FVCSVK  I    GTF++
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

[Close]

BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 122/349 (34.96%), Postives = 190/349 (54.44%), Query Frame = 0


Query: 6    IALERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHL 65
            + LER L Y F  K LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGR 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  S A++ +++EFV  V  E S   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  L----MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
                  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  DSMDTRIKTFVTIDGIDESFEIEG----------AKQKLHDVCSKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D+C ++ W  P+Y   K+ G
Sbjct: 1800 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1859

Query: 306  PSHERIFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H + F   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

[Close]

BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 122/338 (36.09%), Postives = 187/338 (55.33%), Query Frame = 0


Query: 8    LERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHLHQ 67
            L+ +L   F NK LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L  
Sbjct: 1541 LKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTDLPP 1600

Query: 68   GHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRL- 127
            G L+ LRAA V+ E  ARVAV H L+ ++R  S A++ +++EFV  V  E     +    
Sbjct: 1601 GRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFG 1660

Query: 128  ---MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLF 187
                KAPKVL DIVES+AGA+++D  +D   +W + + LL P+ T E L +   PV  L 
Sbjct: 1661 LGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPM--HPVRELQ 1720

Query: 188  QVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMD 247
            + CQ+  + ++ K  + G+ +   V+VDG  +    + QK++A+  AAR AL+ L +  +
Sbjct: 1721 ERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-E 1780

Query: 248  TRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKIA 307
            T  K     DG  ++      +Q L+D+C +R+W  P Y    + GP+H + FV SV++ 
Sbjct: 1781 TATKKEDERDGEKKNGAQMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYSVRVN 1840

Query: 308  TC--YGTFYIVGDEKSRVKDAENSAASLMIRALQERKP 338
            T     T   +G+    VK A++SAA L++  L    P
Sbjct: 1841 TSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNRDFP 1875

[Close]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL
Match: A0A0A0L1E2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 3.1e-182
Identity = 331/338 (97.93%), Postives = 334/338 (98.82%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMD KVKEFVDSV LEGSSVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASV+VDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL
Match: A0A5D3DKE4 (Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00580 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.3e-161
Identity = 298/338 (88.17%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M SSVIA+ERILSY F NK LLEEALTH SY  SASYERLEF+GDSAIGLA+TNYFFLA 
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR++  MD KVKEFVDSV LEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F VCQRNGKQVDIK+WKNGS +IASVYVDG FVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DT+IKT VTI+GIDESFEIEGAKQKLHD C ++KWQKP YS+EK LGPSH R FVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL
Match: A0A1S3BM12 (ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.3e-161
Identity = 298/338 (88.17%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M SSVIA+ERILSY F NK LLEEALTH SY  SASYERLEF+GDSAIGLA+TNYFFLA 
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR++  MD KVKEFVDSV LEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F VCQRNGKQVDIK+WKNGS +IASVYVDG FVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DT+IKT VTI+GIDESFEIEGAKQKLHD C ++KWQKP YS+EK LGPSH R FVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL
Match: A0A6J1HKS4 (ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.7e-145
Identity = 263/335 (78.51%), Postives = 295/335 (88.06%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSV+A+E I+SYNF NK LLEEALTH SY  SASY+RLEF+GDSAIGLA TNYFFLA 
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR + A+D KV+EFVDSV LE +SVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT E+L+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+W NGS +++SV+VDG FVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           D  I+T VTIDGIDESFEIEGAKQKLHD C K+KW KPNYS EKD GPSH++ F CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQER 336
           +T  G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEK 335

[Close]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL
Match: A0A6J1F189 (ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.9e-142
Identity = 258/335 (77.01%), Postives = 292/335 (87.16%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSV+A+E I+SYNF NK LLEEALTH SY  SASY+RLEF+GDSAIGLA TNYFFLA 
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR + A+D KV+EFVDSV LE +S+P
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSIP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT E+L+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+W NGS +++SV+VDG FVASGSS  K IARLNAAREAL K+S +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGIFVASGSSTHKVIARLNAAREALAKMSKTM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           D  I+  VTIDGIDESFEIEGAKQKLHD C K+KW KPNYS EKD GPSH++ F CSVKI
Sbjct: 241 DINIRAAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQER 336
           +   G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+
Sbjct: 301 SPPSGMLYMVGDEKSRVKDSENSAASMMIRALQEK 335

[Close]

BLAST of CSPI04G11360 vs. NCBI nr
Match: XP_031740595.1 (ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical protein Csa_021577 [Cucumis sativus])

HSP 1 Score: 652.5 bits (1682), Expect = 2.0e-183
Identity = 335/338 (99.11%), Postives = 335/338 (99.11%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSV LEGSSV 
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVALEGSSVH 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. NCBI nr
Match: XP_031738803.1 (LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 3.3e-178
Identity = 326/338 (96.45%), Postives = 328/338 (97.04%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYF    
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFXSRF 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
            HLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMD KVKEFVDSV LEGSSVP
Sbjct: 61  SHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVC KRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCCKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. NCBI nr
Match: XP_008449146.1 (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa] >TYK24086.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 578.9 bits (1491), Expect = 2.8e-161
Identity = 298/338 (88.17%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M SSVIA+ERILSY F NK LLEEALTH SY  SASYERLEF+GDSAIGLA+TNYFFLA 
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR++  MD KVKEFVDSV LEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F VCQRNGKQVDIK+WKNGS +IASVYVDG FVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DT+IKT VTI+GIDESFEIEGAKQKLHD C ++KWQKP YS+EK LGPSH R FVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of CSPI04G11360 vs. NCBI nr
Match: KAE8650131.1 (hypothetical protein Csa_010028 [Cucumis sativus])

HSP 1 Score: 529.6 bits (1363), Expect = 1.9e-146
Identity = 284/338 (84.02%), Postives = 298/338 (88.17%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P       + ++A ++  E L        L + +   SP   + +   +  + +  SSVP
Sbjct: 61  P-------TSIKAISLYLELLML-----ALRSLLVLQSPMDCITMLGAIHLLWMI-SSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVC KRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCCKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 325

[Close]

BLAST of CSPI04G11360 vs. NCBI nr
Match: XP_038903239.1 (ribonuclease 3-like protein 2 [Benincasa hispida])

HSP 1 Score: 527.3 bits (1357), Expect = 9.6e-146
Identity = 270/338 (79.88%), Postives = 297/338 (87.87%), Query Frame = 0


Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSV A ERILSYNF NK LLEEALTH SY  SASYERLEF+GDSAIGLAI+NYFFLA 
Sbjct: 1   MESSVRAAERILSYNFKNKKLLEEALTHSSYPDSASYERLEFIGDSAIGLAISNYFFLAF 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P LHQG LSLLRAAN+STEKLARVAV  GLYNYVRR++ A+D KV+EFVDSV LE +SV 
Sbjct: 61  PDLHQGQLSLLRAANISTEKLARVAVRLGLYNYVRRNAAALDDKVREFVDSVALENNSVA 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG L+KAPKVLADIVESVAGAVY+DVNFDLQ+LWVIIRGLLEPIYTLE LQVEPQPVT+L
Sbjct: 121 YGGLVKAPKVLADIVESVAGAVYIDVNFDLQRLWVIIRGLLEPIYTLESLQVEPQPVTLL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+WKNG N++ SVYVDG FVAS SSMQKEIA+L+AAREAL++LS+ M
Sbjct: 181 FEVCQKNGKQVDIKNWKNGPNNVVSVYVDGSFVASSSSMQKEIAKLSAAREALVELSNHM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DT  KT VTIDGIDESFEIEGAK KLHD C K+KW KP YS EKDLGPSHE+ FVCSV I
Sbjct: 241 DTNTKTVVTIDGIDESFEIEGAKHKLHDFCCKKKWPKPTYSTEKDLGPSHEKRFVCSVII 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           +T   T Y++GDEKSRVKDAENSAASLMIRALQER  L
Sbjct: 301 STPSDTLYMLGDEKSRVKDAENSAASLMIRALQERNYL 338

[Close]

BLAST of CSPI04G11360 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 355.1 bits (910), Expect = 6.1e-98
Identity = 188/333 (56.46%), Postives = 253/333 (75.98%), Query Frame = 0


Query: 4   SVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHL 63
           S+ A+E+IL+Y F+NKSLL+EA+TH S +   SYERLEF+GDSAIGLAI+NY +L  P L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR++P++D KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQ 183
            L+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D + +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI-A 303
                + ID      ++  AK KL+++C K+KW KP YSVE+D      + FVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           T   T Y+ GDE+S++K AE+S+A  MIRAL++
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRK 387

[Close]

BLAST of CSPI04G11360 vs. TAIR 10
Match: AT5G45150.1 (RNAse THREE-like protein 3 )

HSP 1 Score: 218.4 bits (555), Expect = 8.9e-57
Identity = 138/384 (35.94%), Postives = 209/384 (54.43%), Query Frame = 0


Query: 3   SSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPH 62
           +SV A+E+IL+Y+F NK+LL+EA+T      S  ++RLEF GDS + +A TNY     P+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAIT----QKSPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYG 122
           L    L  LR ANVS EK AR+AV H L++++   +P++  KVK F ++V  E   VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQ-PVTVLF 182
            L+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT ++L ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 QVCQRNGKQ-----------------------VDIKHWKNGSNSIASVYVDGKFVASGSS 242
           ++  ++G +                       VD++  K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNAAREALIKLSDSMDTRIKTF---------------------------VTID 302
           +  E+++     +      +  D + K F                           + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVK--IATCYGTFYI 334
                 E    + +L ++C+K KW +P +SV+++ GP +E  FVCSVK  I    GTF++
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

[Close]

BLAST of CSPI04G11360 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 122/349 (34.96%), Postives = 190/349 (54.44%), Query Frame = 0


Query: 6    IALERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHL 65
            + LER L Y F  K LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGR 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  S A++ +++EFV  V  E S   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  L----MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
                  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  DSMDTRIKTFVTIDGIDESFEIEG----------AKQKLHDVCSKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D+C ++ W  P+Y   K+ G
Sbjct: 1800 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1859

Query: 306  PSHERIFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H + F   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

[Close]

BLAST of CSPI04G11360 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 122/349 (34.96%), Postives = 190/349 (54.44%), Query Frame = 0


Query: 6    IALERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHL 65
            + LER L Y F  K LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L
Sbjct: 1561 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1620

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGR 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  S A++ +++EFV  V  E S   +  
Sbjct: 1621 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1680

Query: 126  L----MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
                  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1681 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1740

Query: 186  LFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1741 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1800

Query: 246  DSMDTRIKTFVTIDGIDESFEIEG----------AKQKLHDVCSKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D+C ++ W  P+Y   K+ G
Sbjct: 1801 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1860

Query: 306  PSHERIFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H + F   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1861 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1907

[Close]

BLAST of CSPI04G11360 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 150.2 bits (378), Expect = 3.0e-36
Identity = 97/231 (41.99%), Postives = 139/231 (60.17%), Query Frame = 0


Query: 7   ALERILSYNFNNKSLLEEALTHPSY--SASASYERLEFVGDSAIGLAITNYFFLASPHLH 66
           +LE+IL+Y F +KSLL +A T  SY    S SYE LE +GDS + + I   F    P   
Sbjct: 31  SLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEA 90

Query: 67  QGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRL 126
            G L+ LRA NV TEKLARVAV H LY+Y+R   P ++ ++ EFV++  +E   +    L
Sbjct: 91  PGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVEA--MEKYPLHSNGL 150

Query: 127 MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVC 186
           +K PKVLADIVES  GA+++D N   + +W +I+ LLEPI  L+++  +  P+T L ++C
Sbjct: 151 LKVPKVLADIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLDKM--KSHPMTELNEMC 210

Query: 187 Q-RNGKQVDIKHWKNGSNSIASVYVDGKFVASG-SSMQKEIARLNAAREAL 234
           Q RN K      W+   N     +++ KFV  G   ++KE AR  AA+ A+
Sbjct: 211 QKRNLKLTSKDTWE--ENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAI 254

The following BLAST results are available for this feature:

BLAST of CSPI04G11360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTQ0	8.5e-97	56.46	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q6ATG6	1.9e-67	51.26	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]
Q9FKF0	1.2e-55	35.94	Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1	[more]
Q9SP32	1.6e-47	34.96	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q8LMR2	1.6e-47	36.09	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]

BLAST of CSPI04G11360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1E2	3.1e-182	97.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1	[more]
A0A5D3DKE4	1.3e-161	88.17	Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BM12	1.3e-161	88.17	ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1	[more]
A0A6J1HKS4	8.7e-145	78.51	ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 S...	[more]
A0A6J1F189	6.9e-142	77.01	ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4...	[more]

BLAST of CSPI04G11360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740595.1	2.0e-183	99.11	ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical prote...	[more]
XP_031738803.1	3.3e-178	96.45	LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus]	[more]
XP_008449146.1	2.8e-161	88.17	PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclea...	[more]
KAE8650131.1	1.9e-146	84.02	hypothetical protein Csa_010028 [Cucumis sativus]	[more]
XP_038903239.1	9.6e-146	79.88	ribonuclease 3-like protein 2 [Benincasa hispida]	[more]

BLAST of CSPI04G11360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20420.1	6.1e-98	56.46	RNAse THREE-like protein 2	[more]
AT5G45150.1	8.9e-57	35.94	RNAse THREE-like protein 3	[more]
AT1G01040.1	1.2e-48	34.96	dicer-like 1	[more]
AT1G01040.2	1.2e-48	34.96	dicer-like 1	[more]
AT4G15417.1	3.0e-36	41.99	RNAse II-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 262..333 e-value: 8.6E-11 score: 51.8 coord: 176..237 e-value: 0.016 score: 22.1
IPR014720	Double-stranded RNA-binding domain	PFAM	PF00035	dsrm	coord: 176..236 e-value: 7.7E-6 score: 26.5 coord: 262..332 e-value: 2.0E-12 score: 47.6
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 261..334 score: 10.630184
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 175..238 score: 9.586303
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 20..170 e-value: 1.3E-31 score: 121.0
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 38..145 e-value: 6.5E-21 score: 74.9
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 38..46
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 5..147 score: 33.394505
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 20..170 e-value: 1.4655E-39 score: 134.277
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 1..168 e-value: 9.5E-48 score: 164.3
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 3..166
None	No IPR available	GENE3D	3.30.160.20		coord: 172..245 e-value: 7.6E-8 score: 34.2
None	No IPR available	GENE3D	3.30.160.20		coord: 259..335 e-value: 7.4E-18 score: 66.6
None	No IPR available	PANTHER	PTHR14950:SF49	RIBONUCLEASE 3-LIKE PROTEIN 2-RELATED	coord: 7..329
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 7..329
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 129..236
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 261..336

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G11360.1	CSPI04G11360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding