CSPI04G10340 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G10340
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Acetate kinase
Location	Chr4: 8432958 .. 8433574 (+)
RNA-Seq Expression	CSPI04G10340
Synteny	CSPI04G10340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCACAACTCTATAAATGTGCAACTACCATCAGAACAAACCCTCAACAATTATAAAGCCTTGCTTAATTACCTTCGAATGAGTAATAATTCTGTTGCAACGGTAGTCCCTAATGATCGAGAAGATTGCAAACAGATTTCAAATGTTGAGTATAATGAGTATTTGCAAGCAATGGGAAAGTTTGCAGTAGAGGAGCATACACATAACATGAGCGGAATAGAAAGAAAGTTCATCAGTGTGGGGTTTGCGGTGGTTTGTTCAGTGCCTGTGGGAGGAGATAAATATCACCTCCTAGTGAAGGTAGATGAATTTATATTGGCTCCATCATTCCCAAAGTATGAAAAGCTATATATGGCTACTGTGCTTCATAATAAAGTTAATCACTCGTGGGCACTCTTACGATTTGAAAATGTGGCACCTCCTACTGTTTACCCCCCGAGCATTGATTATCTTGATGCCTAGCTCAGTTGTCTACTTTAGATGATGATGCACATGTTGCATTTTTCTTTATCTATGTCAAATATTATATGGGAATATCTAAAATAAAGGATGCTATATATATATGCTTAATCTATCTATGTCTTACTGTTCATTTTTCCACGTGTATTATAAATAAG

mRNA sequence

ATCCACAACTCTATAAATGTGCAACTACCATCAGAACAAACCCTCAACAATTATAAAGCCTTGCTTAATTACCTTCGAATGAGTAATAATTCTGTTGCAACGGTAGTCCCTAATGATCGAGAAGATTGCAAACAGATTTCAAATGTTGAGTATAATGAGTATTTGCAAGCAATGGGAAAGTTTGCAGTAGAGGAGCATACACATAACATGAGCGGAATAGAAAGAAAGTTCATCAGTGTGGGGTTTGCGGTGGTTTGTTCAGTGCCTGTGGGAGGAGATAAATATCACCTCCTAGTGAAGGTAGATGAATTTATATTGGCTCCATCATTCCCAAAGTATGAAAAGCTATATATGGCTACTGTGCTTCATAATAAAGTTAATCACTCGTGGGCACTCTTACGATTTGAAAATGTGGCACCTCCTACTGTTTACCCCCCGAGCATTGATTATCTTGATGCCTAGCTCAGTTGTCTACTTTAGATGATGATGCACATGTTGCATTTTTCTTTATCTATGTCAAATATTATATGGGAATATCTAAAATAAAGGATGCTATATATATATGCTTAATCTATCTATGTCTTACTGTTCATTTTTCCACGTGTATTATAAATAAG

Coding sequence (CDS)

ATGAGTAATAATTCTGTTGCAACGGTAGTCCCTAATGATCGAGAAGATTGCAAACAGATTTCAAATGTTGAGTATAATGAGTATTTGCAAGCAATGGGAAAGTTTGCAGTAGAGGAGCATACACATAACATGAGCGGAATAGAAAGAAAGTTCATCAGTGTGGGGTTTGCGGTGGTTTGTTCAGTGCCTGTGGGAGGAGATAAATATCACCTCCTAGTGAAGGTAGATGAATTTATATTGGCTCCATCATTCCCAAAGTATGAAAAGCTATATATGGCTACTGTGCTTCATAATAAAGTTAATCACTCGTGGGCACTCTTACGATTTGAAAATGTGGCACCTCCTACTGTTTACCCCCCGAGCATTGATTATCTTGATGCCTAG

Protein sequence

MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPPSIDYLDA*

Homology

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BLAST of CSPI04G10340 vs. ExPASy TrEMBL
Match: A0A0A0KW14 (Acetate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G151760 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.2e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC 60
           MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC
Sbjct: 1   MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC 60

Query: 61  SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP 120
           SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP
Sbjct: 61  SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP 120

Query: 121 SIDYLDA 128
           SIDYLDA
Sbjct: 121 SIDYLDA 127

[Close]

BLAST of CSPI04G10340 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.0e-08
Identity = 40/95 (42.11%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 20  ISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVGGDKYHLLVKVDEFI 79
           I N+  + Y+Q +G+FAV EHTHN+ G   +FI V      S  VGG KY L+++V E I
Sbjct: 28  IKNIN-DPYVQELGRFAVMEHTHNLCGASYEFIRVVSGE--SQVVGGKKYCLVIEVKETI 87

Query: 80  LAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAP 115
           + PS P   K + A +L      SWALL F    P
Sbjct: 88  IIPSMPSSIKFFKAIMLDKAWEKSWALLSFVPCEP 119

[Close]

BLAST of CSPI04G10340 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.6e-06
Identity = 36/98 (36.73%), Postives = 52/98 (53.06%), Query Frame = 0


Query: 25  YNEYLQAMGKFAVEEHTHNMSGIERKFISV---GFAVVCSVPVGGDKYHLLVKVDEFILA 84
           +++Y+Q +G+FAV EH H + G+   FI V      +VC     G KY L+V+V E  + 
Sbjct: 29  HDQYVQELGRFAVMEHVHGLCGVGMTFICVVSGESKMVCE----GKKYSLVVEVKEVPVL 88

Query: 85  PSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYP 120
           P      KLY+A VL  +   SW LL F+    P + P
Sbjct: 89  PHACPAIKLYVADVLDKQCAKSWELLAFKPFCLPPMGP 122

[Close]

BLAST of CSPI04G10340 vs. NCBI nr
Match: KGN53820.1 (hypothetical protein Csa_014847 [Cucumis sativus])

HSP 1 Score: 265.4 bits (677), Expect = 2.6e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC 60
           MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC
Sbjct: 1   MSNNSVATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVC 60

Query: 61  SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP 120
           SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP
Sbjct: 61  SVPVGGDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPP 120

Query: 121 SIDYLDA 128
           SIDYLDA
Sbjct: 121 SIDYLDA 127

[Close]

BLAST of CSPI04G10340 vs. NCBI nr
Match: KAE8649343.1 (hypothetical protein Csa_014384 [Cucumis sativus])

HSP 1 Score: 192.6 bits (488), Expect = 2.1e-45
Identity = 92/119 (77.31%), Postives = 104/119 (87.39%), Query Frame = 0


Query: 6   VATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVG 65
           V  VVPN++EDCKQISNVEYN YLQAMG+FAVEE+TH   GI+RKFISVGFAVVCSV VG
Sbjct: 5   VEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVG 64

Query: 66  GDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPPSIDY 125
            +KYHLLVKVDEFILAPS PK+EK+Y+ATVLH KVNHSWALLRFE+ A  TV P +I+Y
Sbjct: 65  EEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTNIEY 123

[Close]

BLAST of CSPI04G10340 vs. NCBI nr
Match: KAG6590492.1 (hypothetical protein SDJN03_15915, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 70.9 bits (172), Expect = 9.3e-09
Identity = 40/99 (40.40%), Postives = 57/99 (57.58%), Query Frame = 0


Query: 16  DCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVGGDKYHLLVKV 75
           D + I N + + Y+  MG+FAV EH HN+ G E KF+SV       VP  G  Y L+++V
Sbjct: 22  DWELIKNKD-DPYVHEMGRFAVMEHAHNLLGAEWKFVSVLSCKTQVVP-KGKLYCLVIEV 81

Query: 76  DEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAP 115
           D  I+ PS+P   +LY A VL  +   SW L+ F+ + P
Sbjct: 82  DIAIVIPSYPHSIELYEAIVLDKQCERSWELISFDPIKP 118

[Close]

BLAST of CSPI04G10340 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 69.7 bits (169), Expect = 2.1e-08
Identity = 40/95 (42.11%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 20  ISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVGGDKYHLLVKVDEFI 79
           I N+  + Y+Q +G+FAV EHTHN+ G   +FI V      S  VGG KY L+++V E I
Sbjct: 28  IKNIN-DPYVQELGRFAVMEHTHNLCGASYEFIRVVSGE--SQVVGGKKYCLVIEVKETI 87

Query: 80  LAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAP 115
           + PS P   K + A +L      SWALL F    P
Sbjct: 88  IIPSMPSSIKFFKAIMLDKAWEKSWALLSFVPCEP 119

[Close]

BLAST of CSPI04G10340 vs. NCBI nr
Match: KAG6590493.1 (hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 65.9 bits (159), Expect = 3.0e-07
Identity = 38/99 (38.38%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 16  DCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVGGDKYHLLVKV 75
           D + I N + + Y+Q MG+FAV EH H + G +  F+ V       VP  G  Y LL+KV
Sbjct: 22  DWELIKNKD-DPYVQEMGRFAVMEHVHGLVGAKWSFVGVVSCESLVVP-KGKLYRLLIKV 81

Query: 76  DEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAP 115
           D  I+ P  P   ++Y A VL  +   SW L+ F+ V P
Sbjct: 82  DIEIVVPCHPHAIEVYEAIVLDKECERSWELMSFKRVLP 118

The following BLAST results are available for this feature:

BLAST of CSPI04G10340 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI04G10340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW14	1.2e-67	100.00	Acetate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G151760 PE=4 SV=1	[more]
A0A6J1BU61	1.0e-08	42.11	cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1GCT2	3.6e-06	36.73	uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC1114530...	[more]

BLAST of CSPI04G10340 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN53820.1	2.6e-67	100.00	hypothetical protein Csa_014847 [Cucumis sativus]	[more]
KAE8649343.1	2.1e-45	77.31	hypothetical protein Csa_014384 [Cucumis sativus]	[more]
KAG6590492.1	9.3e-09	40.40	hypothetical protein SDJN03_15915, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022132557.1	2.1e-08	42.11	cysteine proteinase inhibitor 6-like [Momordica charantia]	[more]
KAG6590493.1	3.0e-07	38.38	hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI04G10340 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 15..112 e-value: 1.3E-7 score: 33.7
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 20..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G10340.1	CSPI04G10340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity