CSPI04G09550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G09550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionendonuclease MutS2 isoform X1
LocationChr4: 7525714 .. 7533440 (-)
RNA-Seq ExpressionCSPI04G09550
SyntenyCSPI04G09550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATCGTTGGTACTATTGGATGAAGTAAGTATGAAAGTCAGTATGGAAACCGTTGAAAAACCATCGTCAATTTTAGTCTAGTTTTTTCTGCTTGGTAGGTTGGTGCAGGGACCAATCCTCTGGAGGGAGCCGCGCTTGGGATGTCACTGCTGGAATCTTTTGCTAAATCTGGCGCTGCATTGACAATTGCAACAACACATCATGGAGAGCTTAAAACCCTAAAATATAGGTACAATATGCAACTTATAGATAGGATTGAAAGCAAAATTTACAGGTTTTGGTTGCTTGACTTTTTAACTTTATAATAGAAACCTTGTTTATTATTATTATCATTTTTTTAAAAAGGAAACAAGCCCTCTCATTAAGATAATGAAATGAGACAATAGTACAATGGAAAGACAACAACTAATCCTAATGATTAGTGAGTGCACCTGGACATGAGACATCTCAACTAGGTTGACACCCCCTTAGCACTCATCATTTTCAATACAAAAATCGATGAAATCAAGTGTTACAAAGTTCAAGTAGAACATTCATACAGAAACAATACAAGAGCTAAAAATATTACAATTTGTAATACAAGAGAAAACAAGACCTTTGAACGAATGCTAAGAGGGAGGAAAAATAAAGGCCTTCAAGTTAAGATTAATTTCTTGGATGGAGAAAGCTTCAAGCTCTTTGTAGAGAGCACACCATGAAGAAGCATTTAGCCAAGCTATATCCGAGTGAATCATCCAAGGGGAGGCTTTGTCATGAAAGAGTCATTGGTTTCTCTCAAACCACAATTCAGCTAACATTGCTTTGATTGCAATATGCCAAATCAATCTTGGTTTTTTCTTTTAAGCTGGGCACTGGTTTCTCTCAAAATGTCACTCTTTGGGGTATCCTCGTAGTGGGTAGGCCAAGATAAGTGGGGGATGAATAGCTTTACAATTCAGCTCGATAATTGCTGTCATCAATATTAAGACCAAAGATTGCTGATTTCTCCCAATTTAGTCTTTGCCCCAAGCGCCATTCAAACAGTTCAATAATTTTCTTCAAATTATCTAACATCTCATCATTGTATTTGCAAAAATTCAAAGTGTCATCAGCATATTGGAGGAAACATGAACTTTGTCTTCTCCCGCAATAAAGCCTTTGAATTTCCATTTTTCATGTATCTTAGCCAGAAGTATATTCAAAACTTCCCTGACTAGAAGGAAAAGGAAAGGTGAGAGAGAATCACCTTGTCTAATATCTCTTGTAGCTTTTATCCTTCCCCTTGGTCTACCATTAATGAAAATTGAGAATTTTGAATTTGACACACAGCCCATTATCCATGAAGACCATATGCGATCAAAGTTTTTTCGGGCAAATTTTTTTCCTACAAAAGCCCAATCTACTCGGTCAAAGGCCTTCTCGAGGTCTAATTTCAGAATCCACCTCTTTTTCTTAGAGCAATATTCTTCCACAGTTCCACTACTTCATTGGCTATAAGAATCGGGTCTATTAATTTGTCTTCCTCCAATAAAGGCCCTTTGAGATAATAATTTTCTTAAGCCCTCGGCTAACACTTTTTACAACTAAACTTATATGTCAAAGTAGTTAAGCTGATTAATAGAAATCTTGTCTAAAAAATAATAAAACTAATAAATCAAAGTAACCTTTTGCATGTACATGCTTTTTTAGTAATTTTTTCCTTTTTCTAGTTCAAAGATCTTTGATTCATGGTTATGAAGTTTTTCTCCACAGCAATGAGGTCTTTGAAAACGCATGTATGGAATTTGATGAGGTGAACCTAAAGCCAACTTACAAGATTCTCTGGGGAATACCAGGTTCTCACTATTGAAATATGATTCACCATTTAATCCTCTCACATTTTATACATTCTCCCCCTCTCATTTTTTAAAACTTATATTCTTCCATTTTGGATAAGAGAAACATTGTTTTCATTTAATTCCGTAATACCATTAGGCACTTAGTATGCAAATATGAGAATGTGATGCCTAGAAATATGACTGTTTAGACATGGAAATAATTCTGTGAAACTTGATTTCAAATTTTATTTATTTTCTTTATAAAATCCAATATTTTTTCTTTTTTGAAATGGAACAAGCCTCTTCATTAGTAAAATGAAATGAGACTAATGGTCAAAATACAAGAGAATTATACAAAGAGCTAAAATTACAAAGGGATAAAGGAGGCGCACCTGGGCATCTCAACTAGGTTGACACCCCCTTAGTGCCCACGTTATATCCGAACTACTAAAACTGAAGAATTAAACATACCCTACATAAGACCAAGTCTCCCAACAACCTGTATAAGTGAGTAACAGATTACACACACAACAAAATTCAAACCAGAGAAGCTGAAAAACTCCAAACAAAGATAATCCAATACAAGTGATATGCTGGAAAAAACAAAAGTGCTCCAAAAGTCTAGGACCATGAAAGTAGCTGTGAAACACTTGGAGAAGATGCCCCAACCAGAGATAATCTGGATGAGCCAAAACAATGTTGAAGCACAAAAAGGAACTCCATTGAGGATAAATCTTCCAGGCCTCTTAATATCCACGAGAGAATCTTATTCAATAGAAGTGAACTGATTTTATAAAATTCCAATAAAGGATTCCCCAGGAATTCCTAGAATTCTTTTTATATTGGTAAACTAAATCTATTAATAATGTTTGATCTGATAAACCACTTCCTAATATATTTTAAACAAATTATTTTAAAATACGAATGAATTCTTAAAATTATAAAAATTTAAAGTAATTAATTTGTGAAGTTAATTACGTTTGGTACGTTTAGGATGTCAATGTCGAATTGTTGAAAGGAGGTCCTATGAAGAAACACTCTGCTCTAGCACAATGCCCTCCTTGATTATGAAGTGATATTTTTACCAATTTAAAACAAGTTTGGCATCTTAGCACCTTTGCAAAATTAGAGATAGATTAGCAAGACAAAGGTAAAGACTTTGTGTGCTTTGTTTGCAATTACATGTAAAGGCTTTTATGTAAAGTGGGAATGCAAATGAGATAATTCAAACCATAAAGATCTGGACCAGGGATTCGGAATCCATTCATTCCTTTGGTTGTTACAAACTTCTCAAGATATGCAATTTTTGCCAATTTTAACTCTCAGCTCACGAACCTAAGTCGTCTCAAGGTACATGTCCTAATGTTATGGTTAATTTCTTACCATCCTAGATGCGTAACTAATTAGGTACAGAAAGCGTCCATATTAATCAAGCAACTGTATTAAGCAAGGTATCTAGTTAAGTTGATCAAATAACCAGATAGCCCTACTTATTAATTAGTCGGTTCTTGAAATTAGTTAATCATGCTTTAAAAGAGCTATTTTAAATAACCTATAAATCACAGCAAGCATGATTTAACTTGTGCCCTTGAGATAATGAAATACGAAGAAACATTGCAATGAATTCCAAGAATAAACGAGAGTTGGCCAAAAATTCAAATATTCAATGCTTAGAATTAGAACTCTGTTAGATACCTAGATTAATATAGAGTAGGGGTATAAAGGTAATTAGATTTATTGTTAGATAGTTGGTTATAAATAGGAAGTTGGGGAAGGAAGAAGGGCATGAAGAATTTGGTGAATAAATAGGGCTGCAACATTCCTTGAAAGAGAGGAAGGGCAAGGGCAGAGGGTTAGGGTTCTTTTACTTGTTCTTTTACTTCTTTTAATATTTTCTATATTTGTATTTTCCTTTTGTGGTTTCTTCAGGTCTATTTATTTTTTATGTAAAAGGTCATATGTACTCTGAATTACTTTTGGTTTATTGAATATATTATATAAAATTATCAAAGTGGATGTCAAATCGACATTCTTGAGGGTCTATGTGGAACAACCATTAGGATTTGAAGCCATGGGTGAAGAGGATAAAGTGCTTCAATTGAAGAAGGATCTTTAAAGTTTGAAGCAAGCACCGAGATCATGGAATTCAAGGATTGACAAGTATTTTAGGGAGAGGAAGTGTGTGAAGTGTCCTTATGAGCATGCCCTATGTATCAAAATGCAAGTTGAAAACATACTAATAATTTGTTTGTGTGTTGACGATCCTTGCATTTTTTATGAATTTAAAAAGGAGATGACGAAGGAGTTTGAAATGACAGACATTGGGTGTCATGAGTTATTATCTTGACAAGTGAAGATGGGATTTTTATATCTCAAGAGGGCTATGCTAGGGAAATGCTTAAAAGGTTTAATATGGATGATGCTAATCCCGTTGGAACACCAATGGAATGTGGAGTCAAAATTACCAAACAGATTGGAGGAGAGAAGGTAGGTTCTACATATTTCAAAAGCTTGGTTGGAAGTTTGAGATACTTGATATACACAAGACGAGTCTTCTTTATTTAGTTGGAATTATTAGTCGATTAATGGAGGCGCTGACAACAATGTATTGCAAAATGGCAAAGAGTATACTTTGTTACATCAAAGGGACAATAGAGAATGGTCTTTCCTATGTCTCTTCTAGCAATTTTGATATTGTTGGTTACTGTGATAGTGATAGGAGTAGTGACCTGGATGATCGAAAGAACACTATGGGGTTTTTATTTTTTATTGGTGAAACAGTGTTCACGTGGATGTCAAAAAAACAACCGATAGTCACATTATCAACATGTGAGGCGGAGTATGTTGCGACAACTTAATGCATATGCCATGCAATTTGGCTAAAAAACCTAACGAAGGAGTTGAAGTTTCAAATGGAGGATCCAATGAAGATTTTTGTTGACAATAAATCAGCCATTGCATTAGCCAAAAATCTAGTATTTCATGGGAGGAACAAACACATTGATACCTGATTTCATTTCCTTCGGGATTGTGTTACAAAGAAAGAAGTGGAACTCAAATATTTCTAGTTGTGAGACCAAGCGGTGGACATTTTCACAAAAGCCTGCAAGCTGGTGTGTTTCTAGGCGTAGCAAATTAAGTTTGAGGGGATGTTGAGAATATGAAAGGTTTCATATTATTAATTAGATTTTGACGGTTTTAGAATTAAATTGATTTGTAACATTTTTTTTTATAACCAATTGTGTCCATTGAGTTTTTTTGGGAGTTTTTAGAAGGTATGACAACTAAATAATGTTATGGTTTCTTAACCATCGAAATTCTATTGTTACATTTTGTAAGTTCAAGGCACGTTTAGTTGAATCAAAATATCACTTTCTTCACACCTGTGTCATCACTCTTTTCTCTTCTAGCGCAGCAAACCTAAATGACGCGAAATTATGTTTTGTTGATTATTTCCTTTTTTGTATTATGTTTTATTGTATTGGTTGTACTATATTTACCAAATTTATTTTTTCCTTCTTTGTAATGGGTTGTTCTTCTATTTAAGAACACATTTCTCTCTTTGTGAAATACATGCTCTGGGTGAGGTCAAATTTATTTTGTCATGTTTGTAGGGCGTTCAAATGCTATAAATATAGCCGAAAGGTTAGGGTTGCCTAGTTCTGTTGTAGATGATGCTCGGGAACTTTATGGTGCGGGAAGTGCACAGATTGATGAGGTAGAATGCACAATTATTGCAAATTTGTGCTTTACTTTTTATGTATGTCTTTTATTTGTGAAGCATCATAAAAAGAAAAGTTTATGAGATACTATCATTATCAATTATTTTGTTATAGTTTACCACTATCCAATAATATTCTTATTTTTGTTCAGTCTGTATTCTTTCTTTAATTTTTTTAAAAAAACTGAAAGGTAGTCCTCGCGTCTCCAGTTGGCAGTTGCAGTAGCAGATATGGAATATTAATTCTTTATGCACTTAGTATATTGCTTCTCAAGATATGTTAGACTACATTATTTACAATTTTATGTTACATTTTAATGCAGGTCATAACCGATATGGAATGTATAAAGAAAAAATATGGAGACCTCTTACAAGAAGCTCAGAATAACTTGACGTAAGTCTCTTAGTAAGATTAATTTTATGGATACAGTCTCTGCCCAACCTAAATCCTTTAATAATATCGTAGCCATAGGTCTGATTCAAATTCTTCTGTGTTTATCAAGTTCTGATCATTGTTAATTCGTATAGTTGCTGAAACTGGGATCTCATTTTTTTTAAAATCATAGCTATTATTATCAGCTTGAAGCAATTTAAAAAAGAAATTAAAAGAACAGGAAAAATGTAATTCCGACCCCTGGCAATGTGGAGGAGAAGATCTTTGGACAAGGCGTTAGTGCTTTTTTATGTTATATCCATATCCAGATAGGTGGTTGAACAAAGTTTTCTTTAATGCCGTATGTACATATGTAATAATAGTCGGTCTCTAGAAAAACTAGCAAGATACAGGTTTGTTTATTGCCTATACCTAACAATTTTACAGGTTTGTTTCTTGCCTATACATAACAATTTTACTTTTAAGTAATGAATAGGGGTCTTTCCTAGTGTTTTAAAAAGCCCTCTCAGGTGCACGTTTAGGCTCAAGGCACAGGTCTAGTGCCTCACCTTGGAAAGACCAGGCTCACAAAATAAGGCGTGCATTAGGCGCTTGCCTTTTGTGAAGCCCCAAGGCTCAAAGCTCGGCTTTTTCATTTTTTAAAGAAATAGTAATAATGAAGGTTTTCCTTTATTAACTAAGGAATCCTAAATGCAAAAATTCTTGTGTTTGAGGTCTTTTTTTTCATATTTCCACTTTCACCATGCTTTCTCCTTTTAACGTAATATTTTGATATGTAGTACGCCTCAGAGAAAAAAGCTTTCACCTTTTTGTGCGCCTTGCATGTAGACTCTAGAAGGCCATTGTGCCTTATTGTGACTTGGGCATTTAAAAACACTAGTTCTTGCTCAAAATGCTAGCTTTAGTCCTTTTTTTTTTTTTTTTTTTGTCATTTTCAATAGATAATGTTCTTGACTAGTAGAATCGTGAATTTTTAACCATCCATATTCTTGTAACCACAGGGAGTCAAAAAATCTTTATGAAAAATTGTTGCTTGCTAGAAGAAACATTATTGAACATGGCAGACGACAGAGGCTTAGAAAAGTGCAAGAGGTAGCCAACGCTGCTACAACCGCTCGTTCTAACCTTCACCAAAAAGGACGAGAACTACGTGCATCTACAATTGAGTACACCCCGCCCTCTGCCATTGATAGGAGGCAACGTGCAGGAATAAACTCTAATAACCGAACTACAGCAGGCAAAAAGGATTTGATGGCTTTGCGTAGGCAAATATCTTCAACCAGTGATATCAGTCAACCACAATCAGGTTTGCCATTGACAAATCTCCTGTTTAATTGTATGTGTTTCGAAGAATTGCAAATTTTCTTCTTAATAGAAGCTCCGTCTTTTATAATTATGTTGTCCGTTTTTATGTGATTAAAAGAATTTTTGTTACCTCAATTTTGTGAGTTTTGTACACTACAGAGGAGCCTTTGTTTCCCACTGTTGGCGATACTGTATATGTTTCTTCCTTCGGAAAAAAAGCGACAGTTTTAGGTGTCGAGCCATCAAAAGACGAAGTCATTGTTCGAGTTGGTAGCATAAAGTTGAAGCTGAAGTTCACCGACATAATGAGATAATCCTCAAACTATCACTAACAAATACCGAACACCAATAATTTCAAATATAATGTTGGTGTATTAGTATTACAATGTTGTGTTGCTACCTTATTTTGAAAATTAATTTATATCACTAGTATAGGAATAGTTCTTAAATTTGCATTTTTTTTTTCGAAGTACACATTGCTACAAAA

mRNA sequence

CAATCGTTGGTACTATTGGATGAAGTAAGTATGAAAGTCAGTATGGAAACCGTTGAAAAACCATCGTCAATTTTAGTCTAGTTTTTTCTGCTTGGTAGGTTGGTGCAGGGACCAATCCTCTGGAGGGAGCCGCGCTTGGGATGTCACTGCTGGAATCTTTTGCTAAATCTGGCGCTGCATTGACAATTGCAACAACACATCATGGAGAGCTTAAAACCCTAAAATATAGCAATGAGGTCTTTGAAAACGCATGTATGGAATTTGATGAGGTGAACCTAAAGCCAACTTACAAGATTCTCTGGGGAATACCAGGGCGTTCAAATGCTATAAATATAGCCGAAAGGTTAGGGTTGCCTAGTTCTGTTGTAGATGATGCTCGGGAACTTTATGGTGCGGGAAGTGCACAGATTGATGAGGTCATAACCGATATGGAATGTATAAAGAAAAAATATGGAGACCTCTTACAAGAAGCTCAGAATAACTTGACGGAGTCAAAAAATCTTTATGAAAAATTGTTGCTTGCTAGAAGAAACATTATTGAACATGGCAGACGACAGAGGCTTAGAAAAGTGCAAGAGGTAGCCAACGCTGCTACAACCGCTCGTTCTAACCTTCACCAAAAAGGACGAGAACTACGTGCATCTACAATTGAGTACACCCCGCCCTCTGCCATTGATAGGAGGCAACGTGCAGGAATAAACTCTAATAACCGAACTACAGCAGGCAAAAAGGATTTGATGGCTTTGCGTAGGCAAATATCTTCAACCAGTGATATCAGTCAACCACAATCAGAGGAGCCTTTGTTTCCCACTGTTGGCGATACTGTATATGTTTCTTCCTTCGGAAAAAAAGCGACAGTTTTAGGTGTCGAGCCATCAAAAGACGAAGTCATTGTTCGAGTTGGTAGCATAAAGTTGAAGCTGAAGTTCACCGACATAATGAGATAATCCTCAAACTATCACTAACAAATACCGAACACCAATAATTTCAAATATAATGTTGGTGTATTAGTATTACAATGTTGTGTTGCTACCTTATTTTGAAAATTAATTTATATCACTAGTATAGGAATAGTTCTTAAATTTGCATTTTTTTTTTCGAAGTACACATTGCTACAAAA

Coding sequence (CDS)

ATGTCACTGCTGGAATCTTTTGCTAAATCTGGCGCTGCATTGACAATTGCAACAACACATCATGGAGAGCTTAAAACCCTAAAATATAGCAATGAGGTCTTTGAAAACGCATGTATGGAATTTGATGAGGTGAACCTAAAGCCAACTTACAAGATTCTCTGGGGAATACCAGGGCGTTCAAATGCTATAAATATAGCCGAAAGGTTAGGGTTGCCTAGTTCTGTTGTAGATGATGCTCGGGAACTTTATGGTGCGGGAAGTGCACAGATTGATGAGGTCATAACCGATATGGAATGTATAAAGAAAAAATATGGAGACCTCTTACAAGAAGCTCAGAATAACTTGACGGAGTCAAAAAATCTTTATGAAAAATTGTTGCTTGCTAGAAGAAACATTATTGAACATGGCAGACGACAGAGGCTTAGAAAAGTGCAAGAGGTAGCCAACGCTGCTACAACCGCTCGTTCTAACCTTCACCAAAAAGGACGAGAACTACGTGCATCTACAATTGAGTACACCCCGCCCTCTGCCATTGATAGGAGGCAACGTGCAGGAATAAACTCTAATAACCGAACTACAGCAGGCAAAAAGGATTTGATGGCTTTGCGTAGGCAAATATCTTCAACCAGTGATATCAGTCAACCACAATCAGAGGAGCCTTTGTTTCCCACTGTTGGCGATACTGTATATGTTTCTTCCTTCGGAAAAAAAGCGACAGTTTTAGGTGTCGAGCCATCAAAAGACGAAGTCATTGTTCGAGTTGGTAGCATAAAGTTGAAGCTGAAGTTCACCGACATAATGAGATAA

Protein sequence

MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSNAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKNLYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDRRQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATVLGVEPSKDEVIVRVGSIKLKLKFTDIMR*
Homology
BLAST of CSPI04G09550 vs. ExPASy Swiss-Prot
Match: P73625 (Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 85/253 (33.60%), Postives = 131/253 (51.78%), Query Frame = 0

Query: 14  LTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSNAINIAERLGLPS 73
           LT+ATTH+GELK LKY +  FENA +EFD+ +L PTY++LWGIPGRSNA+ IA+RLGLP 
Sbjct: 474 LTVATTHYGELKALKYQDARFENASVEFDDQSLSPTYRLLWGIPGRSNALAIAQRLGLPL 533

Query: 74  SVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKNLYEKLLLARRNII 133
           ++V+ A++  G  S  I++VI  +E  +++       AQ  L E++  Y+++     ++ 
Sbjct: 534 AIVEQAKDKLGGFSEDINQVIAGLESQRREQEQKAANAQKLLQETEIFYQQVSQKAASLQ 593

Query: 134 EHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDRRQRAGINSNNRTT 193
              R  +  + QEV  A   A+  + +  R+L+        PSA   +Q   I       
Sbjct: 594 ARERELKSYQDQEVQQAIAAAKEEIAKVIRQLQRG-----KPSAQKAQQATEILG----- 653

Query: 194 AGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATVLGVEPSKDEVIVR 253
                      QI +              PTVG+ + + SFG+ A V  V  +   V V 
Sbjct: 654 -----------QIQAEQKAKVAPKPIGYQPTVGERIRIPSFGQTAEVTQVNATAQTVNVT 705

Query: 254 VGSIKLKLKFTDI 267
           +G +K+ +   DI
Sbjct: 714 LGLMKMTVPMADI 705

BLAST of CSPI04G09550 vs. ExPASy Swiss-Prot
Match: B9KYW4 (Endonuclease MutS2 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) OX=309801 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.4e-25
Identity = 82/268 (30.60%), Postives = 134/268 (50.00%), Query Frame = 0

Query: 2   SLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSN 61
           ++LE   + G AL I TTH+ ELK         ENA +EFD V L PTY++L G+PGRS+
Sbjct: 441 AILERLLEIG-ALGIVTTHYPELKVFATGTPGLENASVEFDPVTLSPTYRLLVGLPGRSH 500

Query: 62  AINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKNL 121
           A+ +A RLGLP  V+  AREL G+G+ Q+D +I +M+   ++   L   A+ +  E+   
Sbjct: 501 ALEVARRLGLPEDVIARARELLGSGAPQLDRLIAEMQRRLEEAESLAAAAERSRREA--- 560

Query: 122 YEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGREL-RASTIEYTPPSAIDR 181
            E+L  A   ++    R+R    QEV        +   +  R++ RA+     PP  +  
Sbjct: 561 -EQLRAAAERLLAEAERERREARQEVLRELEAELARARELARKIERAARSPAYPPIEV-- 620

Query: 182 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 241
                      T    + L  ++R++ S+    Q + E      VGD V +++ G +  V
Sbjct: 621 -----------TQDSLRALEEVKRRVQSSG--RQSRQERVPEIAVGDRVELTALGLEGDV 680

Query: 242 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           + + P  +EV VR+G  +++     + R
Sbjct: 681 VAIHPESEEVEVRIGQFRVRQPQASVRR 688

BLAST of CSPI04G09550 vs. ExPASy Swiss-Prot
Match: Q38YN3 (Endonuclease MutS2 OS=Lactobacillus sakei subsp. sakei (strain 23K) OX=314315 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-24
Identity = 86/266 (32.33%), Postives = 145/266 (54.51%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           +++L+     GA + +A+TH+ ELK   Y+     NA MEFD   L+PTY++L G+PGRS
Sbjct: 430 IAILDQIGIVGANV-VASTHYPELKIYGYNRPQTINASMEFDVATLQPTYRLLIGVPGRS 489

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NA +I+ RLGLP+S+VD A++L    S  ++ +ITD+E  +K      Q  ++ LTE+ +
Sbjct: 490 NAFDISTRLGLPNSIVDQAKQLMNDESQDLNNMITDLENQRKAAETEYQALRHELTEATD 549

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           L+++L  A +   E  R   + K +E ANA          +  E++A  +  T    +  
Sbjct: 550 LHQQLSTAYQQFFE-DRETEMTKAKEKANAIV--------EKAEVKADKV-ITKLRDMQM 609

Query: 181 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 240
            Q A I  N    A K +L  L ++ +   +    +++      VGD V V+S+G++ T+
Sbjct: 610 NQGAQIKENQLIDA-KAELGQLHQETTLKKNKVLQRAKRRQTLKVGDDVLVTSYGQRGTL 669

Query: 241 LGVEPSKDEVIVRVGSIKLKLKFTDI 267
           +    SK+   V++G IK+K+   D+
Sbjct: 670 IRQVDSKNWE-VQMGIIKMKIANDDL 682

BLAST of CSPI04G09550 vs. ExPASy Swiss-Prot
Match: C0Z9F1 (Endonuclease MutS2 OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) OX=358681 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 6.5e-23
Identity = 87/269 (32.34%), Postives = 131/269 (48.70%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MS+++    SGA L +ATTH+ ELK   Y      NA +EFD   L+PTY++L G+PGRS
Sbjct: 430 MSIIDHVIDSGARL-VATTHYSELKAYAYDRPEVINASVEFDVQTLRPTYRLLIGVPGRS 489

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NA  IA RLGLP  ++D AR        Q++ +I  +E  +K        A+    E++ 
Sbjct: 490 NAFAIARRLGLPEHIIDVARGSISEEDNQVESMIASLERNRKSAEADRLAAKAARQEAEE 549

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELR---ASTIEYTPPSA 180
           L  +L   R    E   ++  R   E   A   A+       RELR   A  +E      
Sbjct: 550 LRTQLEEERAQFAEEKNKRMERAEDEARIAVQLAKEEAETIIRELREMMAEGMEIKEHRL 609

Query: 181 IDRRQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKK 240
           ID ++R G    N     +K+ +    +    + I            VGD V V+SFG+K
Sbjct: 610 IDAKKRLG----NAVLELEKEKVKKPAKAVRATQIK-----------VGDEVMVTSFGQK 669

Query: 241 ATVLGVEPSKDEVIVRVGSIKLKLKFTDI 267
            TVL  + + +E +V++G +K+K+K  D+
Sbjct: 670 GTVL-EKVNNEEFLVQIGIMKMKVKRDDM 681

BLAST of CSPI04G09550 vs. ExPASy Swiss-Prot
Match: A5D0W6 (Endonuclease MutS2 OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=mutS2 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-22
Identity = 84/268 (31.34%), Postives = 127/268 (47.39%), Query Frame = 0

Query: 2   SLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSN 61
           S+LE    +GA  T+ATTH+GELK    + E  ENA +EFD + L+PTY++L G PGRSN
Sbjct: 428 SILEKLHSAGAK-TVATTHYGELKDFALTRERVENASVEFDAITLRPTYRLLIGKPGRSN 487

Query: 62  AINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKNL 121
           A  IA RLGLP  VV  AR    A   Q +E++  +E  +++     + A    +E++ L
Sbjct: 488 AFEIAARLGLPEEVVKRARSFLTAEHIQAEELMRSLEKTQQEAEAERRRAAELASEARAL 547

Query: 122 YEKLLLARRNIIEHGRRQRLRKVQEVANA-ATTARSNLHQKGRELRASTIEYTPPSAIDR 181
            E+      ++    R   L K  E A A    AR       RELR    E         
Sbjct: 548 KERYEKIEADLASK-RESILSKAAEEAQALVRAARLEAEAAVRELREKMAE--------- 607

Query: 182 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 241
              A     N     ++ L  L++++           E P     G+ V+++ + +K  V
Sbjct: 608 --EAARERENAIREAREKLRKLQQRVGRAVPEKTVPGEAPAGLRPGEEVFLTRYNQKGYV 667

Query: 242 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           L    +  EV+V+VG IK+ +   ++ R
Sbjct: 668 LEPPGAGGEVLVQVGVIKMNVPLRELRR 682

BLAST of CSPI04G09550 vs. ExPASy TrEMBL
Match: A0A0A0KVZ5 (DNA_MISMATCH_REPAIR_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G114240 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.7e-141
Identity = 266/268 (99.25%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS
Sbjct: 20  MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 79

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 80  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 139

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYT PSAIDR
Sbjct: 140 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTSPSAIDR 199

Query: 181 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 240
            QRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV
Sbjct: 200 MQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 259

Query: 241 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           LGVEPSKDEVIVRVGSIKLKLKFTDIMR
Sbjct: 260 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 287

BLAST of CSPI04G09550 vs. ExPASy TrEMBL
Match: A0A5D3BZK6 (Endonuclease MutS2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold351G00060 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.5e-135
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 570 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 629

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 630 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 689

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 690 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 749

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 750 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 809

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 810 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 838

BLAST of CSPI04G09550 vs. ExPASy TrEMBL
Match: A0A1S3CIG3 (endonuclease MutS2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.5e-135
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 547 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 606

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 607 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 666

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 667 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 726

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 727 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 786

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 787 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 815

BLAST of CSPI04G09550 vs. ExPASy TrEMBL
Match: A0A1S3CJJ8 (endonuclease MutS2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.5e-135
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 486 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 545

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 546 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 605

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 606 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 665

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 666 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 725

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 726 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 754

BLAST of CSPI04G09550 vs. ExPASy TrEMBL
Match: A0A1S3CHZ5 (endonuclease MutS2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.5e-135
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 570 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 629

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 630 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 689

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 690 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 749

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 750 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 809

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 810 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 838

BLAST of CSPI04G09550 vs. NCBI nr
Match: XP_011653396.1 (uncharacterized protein LOC101220812 isoform X2 [Cucumis sativus])

HSP 1 Score: 510.4 bits (1313), Expect = 9.6e-141
Identity = 266/268 (99.25%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS
Sbjct: 569 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 628

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 629 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 688

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYT PSAIDR
Sbjct: 689 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTSPSAIDR 748

Query: 181 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 240
            QRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV
Sbjct: 749 MQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 808

Query: 241 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           LGVEPSKDEVIVRVGSIKLKLKFTDIMR
Sbjct: 809 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 836

BLAST of CSPI04G09550 vs. NCBI nr
Match: XP_004137269.1 (uncharacterized protein LOC101220812 isoform X1 [Cucumis sativus] >KGN53757.2 hypothetical protein Csa_015319 [Cucumis sativus])

HSP 1 Score: 510.4 bits (1313), Expect = 9.6e-141
Identity = 266/268 (99.25%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS
Sbjct: 570 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 629

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 630 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 689

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYT PSAIDR
Sbjct: 690 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTSPSAIDR 749

Query: 181 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 240
            QRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV
Sbjct: 750 MQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 809

Query: 241 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           LGVEPSKDEVIVRVGSIKLKLKFTDIMR
Sbjct: 810 LGVEPSKDEVIVRVGSIKLKLKFTDIMR 837

BLAST of CSPI04G09550 vs. NCBI nr
Match: XP_008462896.1 (PREDICTED: endonuclease MutS2 isoform X2 [Cucumis melo])

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 569 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 628

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 629 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 688

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 689 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 748

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 749 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 808

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 809 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 837

BLAST of CSPI04G09550 vs. NCBI nr
Match: XP_008462897.1 (PREDICTED: endonuclease MutS2 isoform X3 [Cucumis melo])

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 547 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 606

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 607 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 666

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 667 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 726

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 727 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 786

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 787 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 815

BLAST of CSPI04G09550 vs. NCBI nr
Match: XP_008462895.1 (PREDICTED: endonuclease MutS2 isoform X1 [Cucumis melo] >TYK04605.1 endonuclease MutS2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 256/269 (95.17%), Postives = 260/269 (96.65%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWG+PGRS
Sbjct: 570 MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGVPGRS 629

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN
Sbjct: 630 NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 689

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           LYEKLLLARRNIIEHGRRQRLRKVQEVA AATTARSNLHQKGRELRASTIEY+PPSAI R
Sbjct: 690 LYEKLLLARRNIIEHGRRQRLRKVQEVAKAATTARSNLHQKGRELRASTIEYSPPSAIGR 749

Query: 181 RQRAGINSNNRTTAGKKDLMALR-RQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKAT 240
           RQ AGINSNNRTT+GKKDLM L    ISSTSDISQPQSEEP FPTVGDTVYVSSFGKKAT
Sbjct: 750 RQHAGINSNNRTTSGKKDLMGLHTSHISSTSDISQPQSEEPEFPTVGDTVYVSSFGKKAT 809

Query: 241 VLGVEPSKDEVIVRVGSIKLKLKFTDIMR 269
           VLGVEPSKDEVIVRVG+IKLKLKFTDIMR
Sbjct: 810 VLGVEPSKDEVIVRVGTIKLKLKFTDIMR 838

BLAST of CSPI04G09550 vs. TAIR 10
Match: AT5G54090.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-64
Identity = 129/267 (48.31%), Postives = 183/267 (68.54%), Query Frame = 0

Query: 1   MSLLESFAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRS 60
           M++LESFA+SG+ LT+ATTHHGELKTLKYSN  FENACMEFD++NLKPTYKILWG+PGRS
Sbjct: 537 MAILESFAESGSLLTMATTHHGELKTLKYSNSAFENACMEFDDLNLKPTYKILWGVPGRS 596

Query: 61  NAINIAERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQEAQNNLTESKN 120
           NAINIA+RLGLP  +++ ARELYG+ SA+I+EVI DME  K++Y  LL E++  +  S+ 
Sbjct: 597 NAINIADRLGLPCDIIESARELYGSASAEINEVILDMERYKQEYQRLLNESRVYIRLSRE 656

Query: 121 LYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEYTPPSAIDR 180
           L+E LL A++NI +H  ++R +  QE+  A +  RS L +  ++ R+S  + +      +
Sbjct: 657 LHENLLTAQKNINDHSTKERRKMRQELTQAGSMTRSTLRRTLQQFRSSAGKSSQSKVATQ 716

Query: 181 RQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVSSFGKKATV 240
            Q     + +     +   +  RR +        P++     P VG +V+VSS GKKATV
Sbjct: 717 LQTKVKTTKDEDNGIRSSSVVERRPL--------PEAAAQKVPEVGSSVFVSSLGKKATV 776

Query: 241 LGVEPSKDEVIVRVGSIKLKLKFTDIM 268
           L VE SK E++V+VG +K+K+K TD++
Sbjct: 777 LKVEHSKKEILVQVGIMKMKVKLTDVV 795

BLAST of CSPI04G09550 vs. TAIR 10
Match: AT1G65070.1 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 70.9 bits (172), Expect = 1.8e-12
Identity = 65/276 (23.55%), Postives = 122/276 (44.20%), Query Frame = 0

Query: 7   FAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSNAINIA 66
           + K+   + + +TH+G+L  LK +   F+NA MEF    L+PT+++LWG  G SNA+ +A
Sbjct: 503 YIKNRVNVAVVSTHYGDLSRLKDNEPRFQNAAMEFSMETLQPTFRVLWGSTGLSNALRVA 562

Query: 67  ERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQ---EAQNNL-------- 126
           + +G    ++++A +          E +   + +++K G L Q   E +N L        
Sbjct: 563 KSIGFNKRILENAHKW--------TEKLNPEQDVERK-GSLFQSLMEERNKLKLQATKTA 622

Query: 127 ---TESKNLYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEY 186
               +  NLY +L     ++ +  R    ++ Q+V     +A+S + +   E   S +E 
Sbjct: 623 AFHRDLMNLYHELEHESHDLDKRERALLKKETQKVQEDLNSAKSKMERLVAEFE-SQLEI 682

Query: 187 TPPSAIDRRQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVS 246
           T               N+     ++ +  +          S  +      P  G+ V V+
Sbjct: 683 TQAD----------QYNSLILKTEEAVAEIIEACCPMDPDSLEEEYSDYSPQAGEKVLVT 742

Query: 247 SFGKKATVLGVEPSKDE--VIVRVGSIKLKLKFTDI 267
             G K   +  EP  D+  V+V+ G I++++K  DI
Sbjct: 743 GLGDKLGTVVEEPGDDDDTVLVQHGKIRVRIKKKDI 758

BLAST of CSPI04G09550 vs. TAIR 10
Match: AT1G65070.2 (DNA mismatch repair protein MutS, type 2 )

HSP 1 Score: 70.9 bits (172), Expect = 1.8e-12
Identity = 65/276 (23.55%), Postives = 122/276 (44.20%), Query Frame = 0

Query: 7   FAKSGAALTIATTHHGELKTLKYSNEVFENACMEFDEVNLKPTYKILWGIPGRSNAINIA 66
           + K+   + + +TH+G+L  LK +   F+NA MEF    L+PT+++LWG  G SNA+ +A
Sbjct: 503 YIKNRVNVAVVSTHYGDLSRLKDNEPRFQNAAMEFSMETLQPTFRVLWGSTGLSNALRVA 562

Query: 67  ERLGLPSSVVDDARELYGAGSAQIDEVITDMECIKKKYGDLLQ---EAQNNL-------- 126
           + +G    ++++A +          E +   + +++K G L Q   E +N L        
Sbjct: 563 KSIGFNKRILENAHKW--------TEKLNPEQDVERK-GSLFQSLMEERNKLKLQATKTA 622

Query: 127 ---TESKNLYEKLLLARRNIIEHGRRQRLRKVQEVANAATTARSNLHQKGRELRASTIEY 186
               +  NLY +L     ++ +  R    ++ Q+V     +A+S + +   E   S +E 
Sbjct: 623 AFHRDLMNLYHELEHESHDLDKRERALLKKETQKVQEDLNSAKSKMERLVAEFE-SQLEI 682

Query: 187 TPPSAIDRRQRAGINSNNRTTAGKKDLMALRRQISSTSDISQPQSEEPLFPTVGDTVYVS 246
           T               N+     ++ +  +          S  +      P  G+ V V+
Sbjct: 683 TQAD----------QYNSLILKTEEAVAEIIEACCPMDPDSLEEEYSDYSPQAGEKVLVT 742

Query: 247 SFGKKATVLGVEPSKDE--VIVRVGSIKLKLKFTDI 267
             G K   +  EP  D+  V+V+ G I++++K  DI
Sbjct: 743 GLGDKLGTVVEEPGDDDDTVLVQHGKIRVRIKKKDI 758

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P736252.1e-2933.60Endonuclease MutS2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN... [more]
B9KYW41.4e-2530.60Endonuclease MutS2 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2... [more]
Q38YN31.2e-2432.33Endonuclease MutS2 OS=Lactobacillus sakei subsp. sakei (strain 23K) OX=314315 GN... [more]
C0Z9F16.5e-2332.34Endonuclease MutS2 OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) ... [more]
A5D0W61.1e-2231.34Endonuclease MutS2 OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10... [more]
Match NameE-valueIdentityDescription
A0A0A0KVZ54.7e-14199.25DNA_MISMATCH_REPAIR_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A5D3BZK68.5e-13595.17Endonuclease MutS2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CIG38.5e-13595.17endonuclease MutS2 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1[more]
A0A1S3CJJ88.5e-13595.17endonuclease MutS2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1[more]
A0A1S3CHZ58.5e-13595.17endonuclease MutS2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501168 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_011653396.19.6e-14199.25uncharacterized protein LOC101220812 isoform X2 [Cucumis sativus][more]
XP_004137269.19.6e-14199.25uncharacterized protein LOC101220812 isoform X1 [Cucumis sativus] >KGN53757.2 hy... [more]
XP_008462896.11.8e-13495.17PREDICTED: endonuclease MutS2 isoform X2 [Cucumis melo][more]
XP_008462897.11.8e-13495.17PREDICTED: endonuclease MutS2 isoform X3 [Cucumis melo][more]
XP_008462895.11.8e-13495.17PREDICTED: endonuclease MutS2 isoform X1 [Cucumis melo] >TYK04605.1 endonuclease... [more]
Match NameE-valueIdentityDescription
AT5G54090.11.2e-6448.31DNA mismatch repair protein MutS, type 2 [more]
AT1G65070.11.8e-1223.55DNA mismatch repair protein MutS, type 2 [more]
AT1G65070.21.8e-1223.55DNA mismatch repair protein MutS, type 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 101..128
NoneNo IPR availablePANTHERPTHR11361:SF132DNA MISMATCH REPAIR PROTEIN MUTS, TYPE 2coord: 1..265
IPR000432DNA mismatch repair protein MutS, C-terminalSMARTSM00534mutATP5coord: 1..84
e-value: 2.2E-4
score: -60.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..170
e-value: 7.5E-25
score: 89.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 4..84
IPR045076DNA mismatch repair MutS familyPANTHERPTHR11361DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBERcoord: 1..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G09550.1CSPI04G09550.1mRNA
CSPI04G09550.2CSPI04G09550.2mRNA
CSPI04G09550.3CSPI04G09550.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006298 mismatch repair
biological_process GO:0045910 negative regulation of DNA recombination
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0030983 mismatched DNA binding