CSPI04G09320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G09320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Ribonuclease T(2)
Location	Chr4: 7227633 .. 7230578 (-)
RNA-Seq Expression	CSPI04G09320
Synteny	CSPI04G09320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTTATCCATTAAAGCCAAGGCCATGGTGTTTGTTGTTGTTCTTCTTCTTCAGCTGCTGACGGTGGTGTTCGGGTTCTCTGCGGAGGCCCCAAGCAGCAGTGAGCAGAGGGAATTTGATTACTTTGTACTGGCTCTTCAATGGCCAGGTAGTTCTTGCAAAAACCCTGGCAAGTGTTGCCCATCCAACGCCTGCTGCCGTGGGTGAGTAGTTCTCCAAAATACATTGTAAACAAATGGGAACAAACCATCACAATTTACACAAGACAACAAAATAATAATTTTCATTACTGGATGCATGGATGTGTCTAATTTTGTTAATTAATTAGAAAAAGAATATTATTTTAAACCACAAAACCTTGAAAATATTTATATTATATAGTAAAACATATTAAGATATCCAATGTATATCATAAATTTAAAATATTTTCTTTTAGACTTTAGCCACATGCCAGTCTCTTGCCAAATAATTTCCTTTGGAGTTTTGTGTTTTTTTTGTTTTTAACTTCTGAAAGCTAACATTTTTTTTTTCTTCTGTGTGTGCGTAGAGCTGAGTCCCCCACTGAATTTACAATCCGTAAGTGAAATTTAATTAATAAGCTCCTAAGCTTTGGAAGGTTGTCAATTTTATTAGTATTTTTTATTTGGATGTTTAACTCATTTTGTGTATATATGTTTAATACATTGTGATAGATGGATTGTGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAAGCTTTAACGAAAATGAGGTTCCTTAGCTTTCTCTCCATACACTTTTAATTTAAATTGTTTTTTTCCATCTTCAACTTCTCTCACGTATTACATACTATTAAATTTTATGTTGATATATAGATATTTACATGTGCTTGTACATTTCACCTAATATATAAATGTTACATCAATTAATTTATTACAATATCATAGATATTGATGACATACAAAAATAGAAATATTATTATAAATACCGAAACGTACAAAAAAAATTTAAAAGTATATAGCAAAATTTTAAACTATACACTTGCACATTTTGAATTAATGGATTAATAAACCTTCCTACAGTTGAATATTATTTAAATTATTGTTAGTTTTATTTTTAAATCTCCATCCACCTCGATATTGGATATAAAATTGAAGTTTTCAAGTCACTACCATATTCTATACATATTGTAGGTTTTATTTCGTTGAATCTCTCTCCACACATATATATTCAAGAGAATATTTTGATATTTTGATATTATAGGTGAAAGAAGTGAATGATTAATCTTTGAAATGAAAATTTAAGTCTTATTTACTAAGCGACTCATGTTTGCAGATTAATATCTTGACAGAGGATATTCAAAAGTATTGGCCAACTTACAGATGTGGTACTACATCCACATGCCACCAGACGAAGGGATCATTTTGGGCACATGAGGTTCAAACTATTCAACTTTTACTCCTTTCCTTAAGAAGCTCACCAATATCAAAGTTTTGAGACTAACAAATTTTTTCTCTCTTTATTGCCATTACTAATATGATGTGGACCATTTGACATGTTACCCATTGCTATAGTATGGTGAGTTTCTTTTCTTTTTTCTTTTGGAATTCGATATGTGTGTGTGTGTTTTTTAAAAAACTTTTAAGTGTAACTTAAACTTAAGATCAGAATGATTCATAAAAGTAATTATTTTGCAGAGAAGCATGGGACATGTGCAGCTCCTGTGATTGTGGGAGAATATGATTACTTTTTGACAACTATCACCATCTTCTCCAAGTATAATGTCACGGTAGGCTTTTTACTTTTTATTTTTCTTTTTAAAGTATAATTTCTTCTTACCTTTTTTTTATATTTTATCATATGTCTATTAGAATATTGATATAAAAAAACAAATTCAATAGTTGAGTTTGGGTATTAAATTTGGGTGTCTTATTTGTTAACAGAAAGTTTTGAGTGATGCTGGATTTGTAGCTTCCAATACTGAAAAGTATCCTATTGAAGATGTGGTAGCTGCAATTAAGAATGAATTCAATAATGCAACTCCAAAGATTAGTTGTGCTAAAAAGGGGGCTGTCAAAGAACTTTGGTTATGCTTTGACAAAAGCTTTGAGGTAACAGTTTTTTTCCTTCTATTTTGAACCAGTTTTGTAGGCTCTTGATACTATCTTGTATCAACCTAGAGAGTAGTCTTAACAAAATTACATCTTTCTACCCTAAAAGACTTGAATTCTTAGTTTATTTCTAGAAAAAGAAAGACAATTACCTATTTTTTAAAATATTAGTAATAAAATTACAAATAAATAAATAAAGAGGTAAAAGTAGAATGAATATGAGTAAATTTTTCAAATTTTTTAGAAAATCAAATAGTTGTAAAACGTACCCTAATATTCATAATTACAAGATACTAACCAAAGTAAATATGTACTTGTTTGTTACAGCCACGCGACTGCAATCTACCGAATTCATGCCCCGATTTCATCAAGTTCCCAACATATGAACCTCAGGGTATGTTTTTCCTTTCCTTAGTTAAAATTAAGAAACTAATTTTAGAGTTTGATTGCATATGGAATAATGTCATCAAATCATTACTATACTCAAGAGCTTAAGTTAATTAGCTAATAATTAATATATATGATATATACTTAATCCTGTTTAATACATTGCTTTTAACATATTTGTATTATTTACAATGCAGAGCCTAACAAGGTTGAGGGAGCAATTCCATGGATTGCTGATGTTGAAGAAATTATTTAACCTTTTCATTTAAACCTAAAAGAGATGGCTAGCTAGAGGAACCCTTTAATTATTAACATTTTGGCAATGTCTTTTGGGCAATGCCTTTGTGATCTTAAGCTCACCAAATTTCTTTGAGTTGAAAAGATGCATTATGCATGTGGGTTTGCTTTTCCAAATAAAATGGCAGTTGATAATAAAACAATAAGTTGA

mRNA sequence

ATGGCTTCTTTATCCATTAAAGCCAAGGCCATGGTGTTTGTTGTTGTTCTTCTTCTTCAGCTGCTGACGGTGGTGTTCGGGTTCTCTGCGGAGGCCCCAAGCAGCAGTGAGCAGAGGGAATTTGATTACTTTGTACTGGCTCTTCAATGGCCAGGTAGTTCTTGCAAAAACCCTGGCAAGTGTTGCCCATCCAACGCCTGCTGCCGTGGAGCTGAGTCCCCCACTGAATTTACAATCCATGGATTGTGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAAGCTTTAACGAAAATGAGATTAATATCTTGACAGAGGATATTCAAAAGTATTGGCCAACTTACAGATGTGGTACTACATCCACATGCCACCAGACGAAGGGATCATTTTGGGCACATGAGTATGAGAAGCATGGGACATGTGCAGCTCCTGTGATTGTGGGAGAATATGATTACTTTTTGACAACTATCACCATCTTCTCCAAGTATAATGTCACGAAAGTTTTGAGTGATGCTGGATTTGTAGCTTCCAATACTGAAAAGTATCCTATTGAAGATGTGGTAGCTGCAATTAAGAATGAATTCAATAATGCAACTCCAAAGATTAGTTGTGCTAAAAAGGGGGCTGTCAAAGAACTTTGGTTATGCTTTGACAAAAGCTTTGAGCCACGCGACTGCAATCTACCGAATTCATGCCCCGATTTCATCAAGTTCCCAACATATGAACCTCAGGAGCCTAACAAGGTTGAGGGAGCAATTCCATGGATTGCTGATGTTGAAGAAATTATTTAACCTTTTCATTTAAACCTAAAAGAGATGGCTAGCTAGAGGAACCCTTTAATTATTAACATTTTGGCAATGTCTTTTGGGCAATGCCTTTGTGATCTTAAGCTCACCAAATTTCTTTGAGTTGAAAAGATGCATTATGCATGTGGGTTTGCTTTTCCAAATAAAATGGCAGTTGATAATAAAACAATAAGTTGA

Coding sequence (CDS)

ATGGCTTCTTTATCCATTAAAGCCAAGGCCATGGTGTTTGTTGTTGTTCTTCTTCTTCAGCTGCTGACGGTGGTGTTCGGGTTCTCTGCGGAGGCCCCAAGCAGCAGTGAGCAGAGGGAATTTGATTACTTTGTACTGGCTCTTCAATGGCCAGGTAGTTCTTGCAAAAACCCTGGCAAGTGTTGCCCATCCAACGCCTGCTGCCGTGGAGCTGAGTCCCCCACTGAATTTACAATCCATGGATTGTGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAAGCTTTAACGAAAATGAGATTAATATCTTGACAGAGGATATTCAAAAGTATTGGCCAACTTACAGATGTGGTACTACATCCACATGCCACCAGACGAAGGGATCATTTTGGGCACATGAGTATGAGAAGCATGGGACATGTGCAGCTCCTGTGATTGTGGGAGAATATGATTACTTTTTGACAACTATCACCATCTTCTCCAAGTATAATGTCACGAAAGTTTTGAGTGATGCTGGATTTGTAGCTTCCAATACTGAAAAGTATCCTATTGAAGATGTGGTAGCTGCAATTAAGAATGAATTCAATAATGCAACTCCAAAGATTAGTTGTGCTAAAAAGGGGGCTGTCAAAGAACTTTGGTTATGCTTTGACAAAAGCTTTGAGCCACGCGACTGCAATCTACCGAATTCATGCCCCGATTTCATCAAGTTCCCAACATATGAACCTCAGGAGCCTAACAAGGTTGAGGGAGCAATTCCATGGATTGCTGATGTTGAAGAAATTATTTAA

Protein sequence

MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFIKFPTYEPQEPNKVEGAIPWIADVEEII*

Homology

[Close]

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 4.9e-71
Identity = 119/238 (50.00%), Postives = 164/238 (68.91%), Query Frame = 0


Query: 17  LLLQLLTVVFGFSAEA-PSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPT 76
           LLL +  +   F+ +    +  QREFDYF L+LQWPG+ C+    CC  NACCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 77  EFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFW 136
           +FTIHGLWP YN+  WPSCC  + F E EI+ L + ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 137 AHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAI 196
            HE+EKHGTC++PV   EY+YFLTT+ ++ K+NVT VL  AG+VASN+EKYP+  +V AI
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 197 KNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDC------NLPNSCPDFIKFPTYEP 248
           +N F + TP++ C K+ A+ E+ +CF K F+PRDC          SCP ++  P Y P
Sbjct: 187 QNAF-HITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTP 242

[Close]

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.9e-27
Identity = 76/216 (35.19%), Postives = 117/216 (54.17%), Query Frame = 0


Query: 15  VVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESP 74
           ++ LL +L  VF       +SS   +FD+F    QWPGS C    KCC  N+     +  
Sbjct: 10  LISLLVILPSVFS------ASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPA 69

Query: 75  TEFTIHGLWPQYNEKGWPSCCTDAS--FNENEINILTEDIQKYWPTYRCGTTSTCHQTKG 134
            +F IHGLWP Y +  +PS C DAS  F+ + I+ L   ++K WPT  C + S       
Sbjct: 70  ADFGIHGLWPNYKDGTYPSNC-DASKPFDSSTISDLLTSMKKSWPTLACPSGS-----GE 129

Query: 135 SFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVV 194
           +FW HE+EKHGTC+  VI  +++YF T + +  K N+   L+ AG +  + + Y +E + 
Sbjct: 130 AFWEHEWEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYSLESIR 189

Query: 195 AAIKNEFNNATPKISCAKKGA----VKELWLCFDKS 225
            +IK E    TP + C + G+    + +++LC D+S
Sbjct: 190 DSIK-ESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 9.6e-27
Identity = 76/242 (31.40%), Postives = 118/242 (48.76%), Query Frame = 0


Query: 13  FVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAE 72
           F+ +L LQ L V           S  ++FD+F   LQWPG+ C +   CC         +
Sbjct: 4   FIFILALQQLYV----------QSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQT----GK 63

Query: 73  SPTEFTIHGLWPQYNEKGWP-SCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTK 132
              +F IHGLWP Y   GWP +C  D+ F++  ++ L  D+Q+ WPT  C +        
Sbjct: 64  PAADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM---- 123

Query: 133 GSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDV 192
             FW HE+EKHGTCA   +  ++DYF   + +  K N+   L++AG +  + + Y ++D+
Sbjct: 124 -KFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAG-IKPDDKFYEMKDI 183

Query: 193 VAAIKNEFNNATPKISCAKKGA----VKELWLCFDKSFEPRDCNLP----NSCPDFIKFP 246
              IK     A P I C K  +    + +++LC D S   +  N P      C   ++FP
Sbjct: 184 ENTIKQVVGFA-PGIECNKDSSHNSQLYQIYLCVDTS-ASKFINCPVMPHGRCDSRVQFP 222

[Close]

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 115.5 bits (288), Expect = 9.0e-25
Identity = 71/214 (33.18%), Postives = 104/214 (48.60%), Query Frame = 0


Query: 35  SSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSC 94
           SS+  +FD+++   QW  S        C S  C +  E    FTIHGLWP+ ++  +PS 
Sbjct: 26  SSKPGDFDFYLFVQQWIYS-------YCDSQTCIQNKEREA-FTIHGLWPENSDGSYPSF 85

Query: 95  CTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFWAHEYEKHGTCA-APVIVGE 154
           C+  SFN N I  L + +   WP+     T         FW HE+ KHGTC+    I   
Sbjct: 86  CSGPSFNVNAIQDLEDQLNFDWPSLTGPNT--------DFWTHEFSKHGTCSITGPITDI 145

Query: 155 YDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGA 214
           +DYF T I +++++N+T  L       S++  Y   D+  AI   F    P I C+  G 
Sbjct: 146 HDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHF-GGKPGIQCS-SGQ 205

Query: 215 VKELWLCFDK-SFEPRDC-NLPN-SCPDFIKFPT 245
           +  + +C DK S    DC +L   SC   +KFP+
Sbjct: 206 LSTVAVCIDKNSLSIMDCPDLQGWSCSGSVKFPS 221

[Close]

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 2.6e-24
Identity = 71/253 (28.06%), Postives = 125/253 (49.41%), Query Frame = 0


Query: 6   IKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSN 65
           +K++  + + ++++Q L V+   S         ++FD+F    QWP S C     CC   
Sbjct: 2   MKSQKKLLIKIIVVQCLLVLCVTS---------QDFDFFYFVQQWPASYCDTRRSCCYPT 61

Query: 66  ACCRGAESPTEFTIHGLWPQYNEKGWP-SCCTDASFNENEINILTEDIQKYWPTYRCGTT 125
                 +   +F+IHGLWP Y +  WP +C  ++S +E+E + L   ++K WP+  C ++
Sbjct: 62  T----GKPDEDFSIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSS 121

Query: 126 STCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTE 185
                    FW+HE+ KHGTC+A   + ++ YF T +   +K N+ + L++AG    N +
Sbjct: 122 DGL-----KFWSHEWLKHGTCSA---LNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGD 181

Query: 186 KYPIEDVVAAIKNEFNNATPKISC----AKKGAVKELWLCFDKSFEP-RDCNL---PNSC 245
            Y +E +  AI+    + TP I C         + +++LC D S     DC +      C
Sbjct: 182 YYGVESIKKAIEKGVGH-TPFIECNVDSQGNHQLYQVYLCVDSSASKFIDCPIFPHGGKC 232

Query: 246 PDFIKFPTYEPQE 250
              I+FP++   +
Sbjct: 242 GSKIEFPSFSTND 232

[Close]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL
Match: A0A0A0L0P9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.2e-158
Identity = 266/267 (99.63%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MASLSIKAKAMVFVVV+LLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           KFPTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 267

[Close]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL
Match: A0A1S3CHX1 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.2e-142
Identity = 236/267 (88.39%), Postives = 251/267 (94.01%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MAS SIK K MVF + L+++LLTVVFGFSAEAPS SEQREFDYFVLALQWP +SCKN GK
Sbjct: 1   MASFSIKGKPMVF-LFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGA+SPTEFTIHGLWPQYNEKGWPSCCTDASFNENEI IL EDIQKYWPTYR
Sbjct: 61  CCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGT+STCH+TKGSFWAHEYEKHGTCAAPV+VGEYDYFLT +TIFSKYNVTKVLS+ GFVA
Sbjct: 121 CGTSSTCHRTKGSFWAHEYEKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDV+AAIKN+F NATPKI+CAKKG VKELWLCFDKSFEPRDCNL +SCPDFI
Sbjct: 181 SNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           K PTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KLPTYEPQEPNKVEGAIPWIADVEEII 266

[Close]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL
Match: A0A5A7V3T6 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00520 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.3e-139
Identity = 234/267 (87.64%), Postives = 249/267 (93.26%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MAS SIK K MVF + L+++LLTVVFGFSAEAPS SEQREFDYFVLALQWP +SCKN GK
Sbjct: 1   MASFSIKGKPMVF-LFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGA+SPTEFTIHGLWPQYNEKGWPSCCTDASFNENEI IL EDIQKYWPTYR
Sbjct: 61  CCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGT+STCH+TKGSFWAH  EKHGTCAAPV+VGEYDYFLT +TIFSKYNVTKVLS+ GFVA
Sbjct: 121 CGTSSTCHRTKGSFWAH--EKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDV+AAIKN+F NATPKI+CAKKG VKELWLCFDKSFEPRDCNL +SCPDFI
Sbjct: 181 SNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           K PTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KLPTYEPQEPNKVEGAIPWIADVEEII 264

[Close]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL
Match: A0A5D3E236 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold652G00240 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 220/267 (82.40%), Postives = 233/267 (87.27%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MAS SIK K MVF + L+++LLTVVFGFSAEAPS SEQREFDYFVLALQWP +SCKN GK
Sbjct: 1   MASFSIKGKPMVF-LFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGA+SPTEFTIHGLWPQYNEKGWPSCCTDASFNENEI IL EDIQKYWPTYR
Sbjct: 61  CCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           C                  EKHGTCAAPV+VGEYDYFLT +TIFSKYNVTKVLS+ GFVA
Sbjct: 121 C------------------EKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDV+AAIKN+F NATPKI+CAKKG VKELWLCFDKSFEPRDCNL +SCPDFI
Sbjct: 181 SNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           K PTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KLPTYEPQEPNKVEGAIPWIADVEEII 248

[Close]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL
Match: A0A6J1CGP7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 3.9e-124
Identity = 216/271 (79.70%), Postives = 236/271 (87.08%), Query Frame = 0


Query: 1   MASLSIK----AKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCK 60
           MAS SIK    A A VF++V +LQL    F   A AP S EQREFDYFVLALQWP +SC 
Sbjct: 1   MASSSIKGNPTAAATVFLLV-VLQLQAAAFAAGA-APGSEEQREFDYFVLALQWPATSCT 60

Query: 61  NPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYW 120
           NPGKCCPSN CCRGAESPTEFTIHGLWPQY EKGWPSCC+DASF+ENEI  L +DIQKYW
Sbjct: 61  NPGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYW 120

Query: 121 PTYRCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDA 180
           PTYRCGTTSTCH+TKGSFWAHEYEKHGTCAAPVIVGE+DYFLTT+TIFSKYNVTKVL+DA
Sbjct: 121 PTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDA 180

Query: 181 GFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSC 240
           GFVAS+TEKY + DVVAA+++ F NATPKISCAKKGAVKELWLCFDK+F+PRDC+L NSC
Sbjct: 181 GFVASDTEKYALGDVVAAVQDAF-NATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSC 240

Query: 241 PDFIKFPTYEPQEPNKVEGAIPWIADVEEII 268
           PDFIK PTYEPQE NKVEGAIPW+ D E II
Sbjct: 241 PDFIKLPTYEPQEANKVEGAIPWMGDAEAII 268

[Close]

BLAST of CSPI04G09320 vs. NCBI nr
Match: XP_004137162.1 (ribonuclease 2 [Cucumis sativus])

HSP 1 Score: 567.0 bits (1460), Expect = 8.6e-158
Identity = 266/267 (99.63%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MASLSIKAKAMVFVVV+LLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           KFPTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 267

[Close]

BLAST of CSPI04G09320 vs. NCBI nr
Match: KAE8649307.1 (hypothetical protein Csa_015411 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 1.2e-146
Identity = 248/252 (98.41%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MASLSIKAKAMVFVVV+LLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK
Sbjct: 1   MASLSIKAKAMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR
Sbjct: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA
Sbjct: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI
Sbjct: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240

Query: 241 KFPTYEPQEPNK 253
           KFPTYEPQ+ +K
Sbjct: 241 KFPTYEPQDVDK 252

[Close]

BLAST of CSPI04G09320 vs. NCBI nr
Match: XP_008462866.1 (PREDICTED: ribonuclease 2-like [Cucumis melo])

HSP 1 Score: 513.1 bits (1320), Expect = 1.5e-141
Identity = 236/267 (88.39%), Postives = 251/267 (94.01%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MAS SIK K MVF + L+++LLTVVFGFSAEAPS SEQREFDYFVLALQWP +SCKN GK
Sbjct: 1   MASFSIKGKPMVF-LFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGA+SPTEFTIHGLWPQYNEKGWPSCCTDASFNENEI IL EDIQKYWPTYR
Sbjct: 61  CCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGT+STCH+TKGSFWAHEYEKHGTCAAPV+VGEYDYFLT +TIFSKYNVTKVLS+ GFVA
Sbjct: 121 CGTSSTCHRTKGSFWAHEYEKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDV+AAIKN+F NATPKI+CAKKG VKELWLCFDKSFEPRDCNL +SCPDFI
Sbjct: 181 SNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           K PTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KLPTYEPQEPNKVEGAIPWIADVEEII 266

[Close]

BLAST of CSPI04G09320 vs. NCBI nr
Match: XP_038876700.1 (ribonuclease 2-like [Benincasa hispida])

HSP 1 Score: 505.4 bits (1300), Expect = 3.1e-139
Identity = 234/268 (87.31%), Postives = 249/268 (92.91%), Query Frame = 0


Query: 1   MASLSIKAKAMVF-VVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPG 60
           MAS SIKAK M   VV LLLQLL V FGF+A APS SEQREFDYFVLALQWP +SCKNPG
Sbjct: 1   MASFSIKAKPMAAPVVFLLLQLLAVAFGFTAVAPSGSEQREFDYFVLALQWPATSCKNPG 60

Query: 61  KCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTY 120
           KCCP+N CCRG +SPTEFTIHGLWPQYN++GWPSCCTDA+FNENEINIL EDIQKYWPTY
Sbjct: 61  KCCPANGCCRGVDSPTEFTIHGLWPQYNDRGWPSCCTDANFNENEINILAEDIQKYWPTY 120

Query: 121 RCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFV 180
           RCGTTSTCH+TKGSFWAHEYEKHGTCAAPVIVGEYDYFLTT+TIFSKYNVTKVL+DAGFV
Sbjct: 121 RCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKVLNDAGFV 180

Query: 181 ASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDF 240
           ASNTEKYPIEDVVAAIKNEF N+TPKI+CAKKG VKELW+CFDKSFEPRDC+L NSCPDF
Sbjct: 181 ASNTEKYPIEDVVAAIKNEF-NSTPKITCAKKGVVKELWICFDKSFEPRDCDLQNSCPDF 240

Query: 241 IKFPTYEPQEPNKVEGAIPWIADVEEII 268
           IK PTY+PQEPNKVEGAIPWIAD EEII
Sbjct: 241 IKLPTYDPQEPNKVEGAIPWIADAEEII 267

[Close]

BLAST of CSPI04G09320 vs. NCBI nr
Match: KAA0061176.1 (ribonuclease 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 503.8 bits (1296), Expect = 9.0e-139
Identity = 234/267 (87.64%), Postives = 249/267 (93.26%), Query Frame = 0


Query: 1   MASLSIKAKAMVFVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGK 60
           MAS SIK K MVF + L+++LLTVVFGFSAEAPS SEQREFDYFVLALQWP +SCKN GK
Sbjct: 1   MASFSIKGKPMVF-LFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKNAGK 60

Query: 61  CCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYR 120
           CCPSNACCRGA+SPTEFTIHGLWPQYNEKGWPSCCTDASFNENEI IL EDIQKYWPTYR
Sbjct: 61  CCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWPTYR 120

Query: 121 CGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVA 180
           CGT+STCH+TKGSFWAH  EKHGTCAAPV+VGEYDYFLT +TIFSKYNVTKVLS+ GFVA
Sbjct: 121 CGTSSTCHRTKGSFWAH--EKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGGFVA 180

Query: 181 SNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSCPDFI 240
           SNTEKYPIEDV+AAIKN+F NATPKI+CAKKG VKELWLCFDKSFEPRDCNL +SCPDFI
Sbjct: 181 SNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCPDFI 240

Query: 241 KFPTYEPQEPNKVEGAIPWIADVEEII 268
           K PTYEPQEPNKVEGAIPWIADVEEII
Sbjct: 241 KLPTYEPQEPNKVEGAIPWIADVEEII 264

[Close]

BLAST of CSPI04G09320 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 269.2 bits (687), Expect = 3.5e-72
Identity = 119/238 (50.00%), Postives = 164/238 (68.91%), Query Frame = 0


Query: 17  LLLQLLTVVFGFSAEA-PSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPT 76
           LLL +  +   F+ +    +  QREFDYF L+LQWPG+ C+    CC  NACCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 77  EFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFW 136
           +FTIHGLWP YN+  WPSCC  + F E EI+ L + ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 137 AHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAI 196
            HE+EKHGTC++PV   EY+YFLTT+ ++ K+NVT VL  AG+VASN+EKYP+  +V AI
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 197 KNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDC------NLPNSCPDFIKFPTYEP 248
           +N F + TP++ C K+ A+ E+ +CF K F+PRDC          SCP ++  P Y P
Sbjct: 187 QNAF-HITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTP 242

[Close]

BLAST of CSPI04G09320 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 208.8 bits (530), Expect = 5.6e-54
Identity = 100/238 (42.02%), Postives = 137/238 (57.56%), Query Frame = 0


Query: 17  LLLQLLTVVFGFSAEA-PSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPT 76
           LLL +  +   F+ +    +  QREFDYF L+LQWPG+ C+    CC  NACCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 77  EFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFW 136
           +FTIHGLWP YN+  WPSCC  + F E EI+ L + ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 137 AHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAI 196
            HE                                 VL  AG+VASN+EKYP+  +V AI
Sbjct: 127 GHE--------------------------------DVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 197 KNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDC------NLPNSCPDFIKFPTYEP 248
           +N F + TP++ C K+ A+ E+ +CF K F+PRDC          SCP ++  P Y P
Sbjct: 187 QNAF-HITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTP 210

[Close]

BLAST of CSPI04G09320 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-29
Identity = 73/219 (33.33%), Postives = 115/219 (52.51%), Query Frame = 0


Query: 35  SSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPS- 94
           S  Q +FD+F   LQWPG+ C     CC   +     +   +F IHGLWP YN   WPS 
Sbjct: 22  SRPQEDFDFFYFVLQWPGAYCDTSRACCYPTS----GKPAADFGIHGLWPNYNGGSWPSN 81

Query: 95  CCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGE 154
           C  D+ F+ ++I+ L   ++K WPT  C +         +FW HE+EKHGTC+  V + +
Sbjct: 82  CDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGF-----NFWEHEWEKHGTCSESV-MDQ 141

Query: 155 YDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVVAAIKNEFNNATPKISCAK--- 214
           ++YF   + +  K N+ ++L ++G +  +   Y ++ +  AIK+     TP I C K   
Sbjct: 142 HEYFENALKLKQKANLLQILKNSG-INPDDGFYNLDKITNAIKDGI-GFTPGIECNKDPE 201

Query: 215 -KGAVKELWLCFDKS-FEPRDCN-LP-NSCPDFIKFPTY 246
               + ++++C D S  E  +C  LP  SCP  I+F  +
Sbjct: 202 RNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

[Close]

BLAST of CSPI04G09320 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 124.4 bits (311), Expect = 1.4e-28
Identity = 76/216 (35.19%), Postives = 117/216 (54.17%), Query Frame = 0


Query: 15  VVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAESP 74
           ++ LL +L  VF       +SS   +FD+F    QWPGS C    KCC  N+     +  
Sbjct: 10  LISLLVILPSVFS------ASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPA 69

Query: 75  TEFTIHGLWPQYNEKGWPSCCTDAS--FNENEINILTEDIQKYWPTYRCGTTSTCHQTKG 134
            +F IHGLWP Y +  +PS C DAS  F+ + I+ L   ++K WPT  C + S       
Sbjct: 70  ADFGIHGLWPNYKDGTYPSNC-DASKPFDSSTISDLLTSMKKSWPTLACPSGS-----GE 129

Query: 135 SFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDVV 194
           +FW HE+EKHGTC+  VI  +++YF T + +  K N+   L+ AG +  + + Y +E + 
Sbjct: 130 AFWEHEWEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYSLESIR 189

Query: 195 AAIKNEFNNATPKISCAKKGA----VKELWLCFDKS 225
            +IK E    TP + C + G+    + +++LC D+S
Sbjct: 190 DSIK-ESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of CSPI04G09320 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 122.1 bits (305), Expect = 6.8e-28
Identity = 76/242 (31.40%), Postives = 118/242 (48.76%), Query Frame = 0


Query: 13  FVVVLLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCKNPGKCCPSNACCRGAE 72
           F+ +L LQ L V           S  ++FD+F   LQWPG+ C +   CC         +
Sbjct: 4   FIFILALQQLYV----------QSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQT----GK 63

Query: 73  SPTEFTIHGLWPQYNEKGWP-SCCTDASFNENEINILTEDIQKYWPTYRCGTTSTCHQTK 132
              +F IHGLWP Y   GWP +C  D+ F++  ++ L  D+Q+ WPT  C +        
Sbjct: 64  PAADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM---- 123

Query: 133 GSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDAGFVASNTEKYPIEDV 192
             FW HE+EKHGTCA   +  ++DYF   + +  K N+   L++AG +  + + Y ++D+
Sbjct: 124 -KFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAG-IKPDDKFYEMKDI 183

Query: 193 VAAIKNEFNNATPKISCAKKGA----VKELWLCFDKSFEPRDCNLP----NSCPDFIKFP 246
              IK     A P I C K  +    + +++LC D S   +  N P      C   ++FP
Sbjct: 184 ENTIKQVVGFA-PGIECNKDSSHNSQLYQIYLCVDTS-ASKFINCPVMPHGRCDSRVQFP 222

The following BLAST results are available for this feature:

BLAST of CSPI04G09320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42814	4.9e-71	50.00	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]
P42813	1.9e-27	35.19	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	9.6e-27	31.40	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q7M438	9.0e-25	33.18	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
P80196	2.6e-24	28.06	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of CSPI04G09320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0P9	4.2e-158	99.63	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1	[more]
A0A1S3CHX1	7.2e-142	88.39	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1	[more]
A0A5A7V3T6	4.3e-139	87.64	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00...	[more]
A0A5D3E236	5.0e-127	82.40	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold652G00...	[more]
A0A6J1CGP7	3.9e-124	79.70	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1	[more]

BLAST of CSPI04G09320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137162.1	8.6e-158	99.63	ribonuclease 2 [Cucumis sativus]	[more]
KAE8649307.1	1.2e-146	98.41	hypothetical protein Csa_015411 [Cucumis sativus]	[more]
XP_008462866.1	1.5e-141	88.39	PREDICTED: ribonuclease 2-like [Cucumis melo]	[more]
XP_038876700.1	3.1e-139	87.31	ribonuclease 2-like [Benincasa hispida]	[more]
KAA0061176.1	9.0e-139	87.64	ribonuclease 2-like [Cucumis melo var. makuwa]	[more]

BLAST of CSPI04G09320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39780.1	3.5e-72	50.00	ribonuclease 2	[more]
AT2G39780.2	5.6e-54	42.02	ribonuclease 2	[more]
AT1G14220.1	1.6e-29	33.33	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.4e-28	35.19	ribonuclease 1	[more]
AT1G26820.1	6.8e-28	31.40	ribonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 37..247 e-value: 5.1E-72 score: 244.2
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 40..245
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 41..230 e-value: 5.7E-47 score: 160.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 32..251
None	No IPR available	PANTHER	PTHR11240:SF66	OS01G0897300 PROTEIN	coord: 32..251
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 134..145
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 77..84
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 41..242 e-value: 1.82506E-59 score: 185.614

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G09320.1	CSPI04G09320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding