CSPI04G08400 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G08400
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionIntegrase catalytic domain-containing protein
LocationChr4: 6140236 .. 6142965 (+)
RNA-Seq ExpressionCSPI04G08400
SyntenyCSPI04G08400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACCATTTCCTCAATCTAAAAATCATGTTTACATCTTGTTGGCTGAAGACTATGTTTCTAAGTGGGTTGAAGCAATTTCCTGTGTTAAGAATGATGTAGTTACAGTGAGTAGATTTCTGAAAAAGAACATCCTTACACATTTTGGGACCCCTAGAGCAATTATCAGCGATGAAGGACTCCATTTCGTTAATCATATCATCACTAAGGTGCTTGCAAAGTACAATATAAGACATAAGATAGACATTGCCTATCATCCGCAAATAAATAGTCAAGCAGAAGTATCCAACAGAGAAATTAAGAAGATCTTAGAAAAATTGGTAAATCATTCCTGCAAGGATTGGGTAGATCACCTAGACTCTGCGCTTTGGGCATATCGTACAGCGTACAAGACGCCAATTGGGATGTCCCCTTATGGGATAGTTTTTAGGAAAGCTTGCCACTTACCGCTAGAATTGAAACACAAGGCGTTATAGGCATGCAAAAAGTTGAACTTTGGCCATCGCGCCACAGGAAGACAAGATTACTATAATTGCACAACCTTCAAGAATGGCATTCTCAAGCATATGAAAATGGTAAGATTTACAAGACAAAGACTAAGGCTTGGCATAATAACCACATTCATAAACACGAACTTCATATTGGACAAAAAGTATTGTTATTCAATTCTCGTCTATGACTATTTCCGAGTAAGCTCCATTTGCGATGGTCTGAACCATTAAGCAAATCTTTCTATAGAGATTGCTTCACTAGACGGAACAAATGTGTTTAAGGTAAATGGACAATGACTCAAGGCATACCTTGAAGAAGAACAATGTAATAAATGTTCTGTGGATTTATTGTAAAACGTCTTTTAGTACTTACCTTCAAAACGCATAAATGCATTTTGTATCTTTCAACAAGATCATATCATTTATGCATAAAGCATTGTTCTGTGCATCACTTTCGCATTATTTTCATTTTTGTTTGCTTTACTTTCATTTACATCGTATTCTTTATTGCATTCTTTACTACTTTCTTTATCACATATCTTTATGCCTTTGTAACTTAGGAACATTTGTGCCTATGGGAAAACTCAGGAAGCTACACAATAGGATTTAGTTAGGAAAAGTTATAATTCAAACCTAATTCTTCAGGAGTATAGTGTAAGGGAATTGAAGCATTTTTAAGCAGGAACGACGTCTAGGGTTTTGAAGGGACGTGTCAACCTTTTGTCAGACACATTAAATGCAGAAACAACCGTACTTATTGCCTTGGCGTGTATAAATATGAACAAAACTGTTCTTCTCCCTTTTTATTCTTTCATTAGACTCTTAAGTAAACCTCAGAAAACTCATCACCTTCAACCATCCTTCATTTTTCACCTGAAAACTCATCACCTTCAACCATCTTTCACTTTCAAGTGGCTGGCACATCTAAAAACACTCCTGAAATTGCCCAAACCCTTAAGAAACCTCAAAAATCTCTTTATAGAAATCCAAACTGAGGATTCTAGGAAGAAAATAGTCTTCAAACCACAAAACCAGTCGTCACCCTAAACCAACTCTGTTAGATCTTATTCGGAGGAAGCTCAAAAACCTAACCTAACCGCTAGAGACTCCATTCTTGAAGTTAATCAATGGATGAGGCAAGAAGAGAAATTTTATACTGAATTATCAGACTTAGGCACAGGGGTGGAAACCACCCGTTTAGCAGGCAATTCGTCGTGTTACACGCATTTGAAGCATGGGGCGGGGAAAGTGCTTGTTGACTGCAAGGAGATAAGAGGAGAGGCAAGTTTGCAAATTCCATCACACCTCTTCGATCAAGTTAAGAAATTCATAGGACATGAGCGAGTAGGTAGAAAGGGTTGTCGTAGCTAATGGGGAGACAGAACAGTATGAGGTTGATCCTATGGATATTGTGGTAGAAGAGTTAACATAGGAAGTTGGCAGGATGAGCTCTATAGTGTGATTGGTAAGACCTGATGACCCTGGACTCCCAAGCTCCCAATGTTCGCAAAAAGGTAAAAGTAAAGCGGGAACGTCCAGACCTGGGGATGTTAAAAAAAGGGAAAATGGTGGGCACCCCTGAACTAGACAAGCTCATTAAGATCGAAAAGGGATTACTACCCTTTAAGGGCCCACTACCTGACTTCCTTCCTGACCCAATTAAGGCGTTCAAATGGAAAAAGTTCTTTATCGGTGAGATATAAATACAATTAGATCTAGTAGATATGTTCTACGCGATAAAGTTTCACCCTGAAGAATCATATATCGTAGTGGAAGGAGACAAAGTTCCCTCCACCGTAAAAGCGATTAGCAAGTTGTATGATTTACCTAATGGTTCTTACGCATATCCCGACCAAAGAATTATTGACAACCCAATGAGGAGTGATGTGAAAAAAATTATTTAGTTAATCGCGTGGCCAGGGGCTGATGGATAGAAACACCAGCTGGGAGGCTTCAATTATTCCCGCATCAATTAACAACTGAGGCAAACGTCTCATTGGTCTTCATAAAAATAAGATGCTCCCAACTCGCCATGACAGCATAGTTTCCATTGAACATGAGTTAGTTCTATACTATATCTTGATGAAGCAGCCATTTAATTTGAGGAGTATAATTAATGGAGCTCTCCTTGTCTGGAGGAGGAACCCTAAGGGCGCAAAGCCTTTTCCGTCTACCATGGAGAAGTTATGTTTGAAGTACTTGCCCACCCTCGCGAGATACCACAAACTCCCATGGTGA

mRNA sequence

ATGAGACCATTTCCTCAATCTAAAAATCATGTTTACATCTTGTTGGCTGAAGACTATGTTTCTAAGTGGGTTGAAGCAATTTCCTGTGTTAAGAATGATGTAGTTACAGTGAGTAGATTTCTGAAAAAGAACATCCTTACACATTTTGGGACCCCTAGAGCAATTATCAGCGATGAAGGACTCCATTTCGTTAATCATATCATCACTAAGGTGCTTGCAAAGTACAATATAAGACATAAGATAGACATTGCCTATCATCCGCAAATAAATAGTCAAGCAGAAGTATCCAACAGAGAAATTAAGAAGATCTTAGAAAAATTGGTAAATCATTCCTGCAAGGATTGGGTAGATCACCTAGACTCTGCGCTTTGGGCATATCGTACAGCGTACAAGACGCCAATTGGGATGTCCCCTTATGGGATAGTTTTTAGGAAAGCTTGCCACTTACCGCTAGAATTGAAACACAAGGCATCTTATTCGGAGGAAGCTCAAAAACCTAACCTAACCGCTAGAGACTCCATTCTTGAAGTTAATCAATGGATGAGGCAAGAAGAGAAATTTTATACTGAATTATCAGACTTAGGCACAGGGGTGGAAACCACCCGTTTAGCAGGCAATTCGTCGTGTTACACGCATTTGAAGCATGGGGCGGGGAAAGTGCTTGTTGACTGCAAGGAGATAAGAGGAGAGGCAAGTTTGCAAATTCCATCACACCTCTTCGATCAAGTTAAGAAATTCATAGGACATGAGCGAGTAGACCTGATGACCCTGGACTCCCAAGCTCCCAATGTTCGCAAAAAGGTAAAAGTAAAGCGGGAACGTCCAGACCTGGGGATGTTAAAAAAAGGGAAAATGGTGGGCACCCCTGAACTAGACAAGCTCATTAAGATCGAAAAGGGATTACTACCCTTTAAGGGCCCACTACCTGACTTCCTTCCTGACCCAATTAAGGCGTTCAAATGGAAAAAGTTCTTTATCGTGGAAGGAGACAAAGTTCCCTCCACCGTAAAAGCGATTAGCAAGTTGTATGATTTACCTAATGGTTCTTACGCATATCCCGACCAAAGAATTATTGACAACCCAATGAGGAGTGATATGCTCCCAACTCGCCATGACAGCATAGTTTCCATTGAACATGAGTTAGTTCTATACTATATCTTGATGAAGCAGCCATTTAATTTGAGGAGTATAATTAATGGAGCTCTCCTTGTCTGGAGGAGGAACCCTAAGGGCGCAAAGCCTTTTCCGTCTACCATGGAGAAGTTATGTTTGAAGTACTTGCCCACCCTCGCGAGATACCACAAACTCCCATGGTGA

Coding sequence (CDS)

ATGAGACCATTTCCTCAATCTAAAAATCATGTTTACATCTTGTTGGCTGAAGACTATGTTTCTAAGTGGGTTGAAGCAATTTCCTGTGTTAAGAATGATGTAGTTACAGTGAGTAGATTTCTGAAAAAGAACATCCTTACACATTTTGGGACCCCTAGAGCAATTATCAGCGATGAAGGACTCCATTTCGTTAATCATATCATCACTAAGGTGCTTGCAAAGTACAATATAAGACATAAGATAGACATTGCCTATCATCCGCAAATAAATAGTCAAGCAGAAGTATCCAACAGAGAAATTAAGAAGATCTTAGAAAAATTGGTAAATCATTCCTGCAAGGATTGGGTAGATCACCTAGACTCTGCGCTTTGGGCATATCGTACAGCGTACAAGACGCCAATTGGGATGTCCCCTTATGGGATAGTTTTTAGGAAAGCTTGCCACTTACCGCTAGAATTGAAACACAAGGCATCTTATTCGGAGGAAGCTCAAAAACCTAACCTAACCGCTAGAGACTCCATTCTTGAAGTTAATCAATGGATGAGGCAAGAAGAGAAATTTTATACTGAATTATCAGACTTAGGCACAGGGGTGGAAACCACCCGTTTAGCAGGCAATTCGTCGTGTTACACGCATTTGAAGCATGGGGCGGGGAAAGTGCTTGTTGACTGCAAGGAGATAAGAGGAGAGGCAAGTTTGCAAATTCCATCACACCTCTTCGATCAAGTTAAGAAATTCATAGGACATGAGCGAGTAGACCTGATGACCCTGGACTCCCAAGCTCCCAATGTTCGCAAAAAGGTAAAAGTAAAGCGGGAACGTCCAGACCTGGGGATGTTAAAAAAAGGGAAAATGGTGGGCACCCCTGAACTAGACAAGCTCATTAAGATCGAAAAGGGATTACTACCCTTTAAGGGCCCACTACCTGACTTCCTTCCTGACCCAATTAAGGCGTTCAAATGGAAAAAGTTCTTTATCGTGGAAGGAGACAAAGTTCCCTCCACCGTAAAAGCGATTAGCAAGTTGTATGATTTACCTAATGGTTCTTACGCATATCCCGACCAAAGAATTATTGACAACCCAATGAGGAGTGATATGCTCCCAACTCGCCATGACAGCATAGTTTCCATTGAACATGAGTTAGTTCTATACTATATCTTGATGAAGCAGCCATTTAATTTGAGGAGTATAATTAATGGAGCTCTCCTTGTCTGGAGGAGGAACCCTAAGGGCGCAAAGCCTTTTCCGTCTACCATGGAGAAGTTATGTTTGAAGTACTTGCCCACCCTCGCGAGATACCACAAACTCCCATGGTGA

Protein sequence

MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLDSALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYSEEAQKPNLTARDSILEVNQWMRQEEKFYTELSDLGTGVETTRLAGNSSCYTHLKHGAGKVLVDCKEIRGEASLQIPSHLFDQVKKFIGHERVDLMTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPDPIKAFKWKKFFIVEGDKVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSIVSIEHELVLYYILMKQPFNLRSIINGALLVWRRNPKGAKPFPSTMEKLCLKYLPTLARYHKLPW*
Homology
BLAST of CSPI04G08400 vs. ExPASy Swiss-Prot
Match: P03359 (Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 49/135 (36.30%), Postives = 68/135 (50.37%), Query Frame = 0

Query: 12   YILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFVNHIITKV 71
            Y+L+  D  S WVEA        +TV + + + IL  FG P+ + SD G  FV  +   +
Sbjct: 1418 YLLVFIDTFSGWVEAFPTKTETALTVCKKILEEILPRFGIPKVLGSDNGPAFVAQVSQGL 1477

Query: 72   LAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKL-VNHSCKDWVDHLDSALWAYRTAY 131
              +  I  K+  AY PQ + Q E  NR IK+ L KL +    KDWV  L  AL   R   
Sbjct: 1478 ATQLGINWKLHCAYRPQSSGQVERMNRTIKETLTKLALETGXKDWVALLPLALLRAR--- 1537

Query: 132  KTP--IGMSPYGIVF 144
             TP   G++PY I++
Sbjct: 1538 NTPGRFGLTPYEILY 1549

BLAST of CSPI04G08400 vs. ExPASy Swiss-Prot
Match: P21414 (Gag-Pol polyprotein OS=Gibbon ape leukemia virus OX=11840 GN=pol PE=3 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 6.4e-12
Identity = 49/142 (34.51%), Postives = 69/142 (48.59%), Query Frame = 0

Query: 5    PQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFV 64
            P    + Y+L+  D  S WVEA        + V + + + IL  FG P+ + SD G  FV
Sbjct: 1410 PGRYGNKYLLVFIDTFSGWVEAFPTKTETALIVCKKILEEILPRFGIPKVLGSDNGPAFV 1469

Query: 65   NHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKL-VNHSCKDWVDHLDSAL 124
              +   +  +  I  K+  AY PQ + Q E  NR IK+ L KL +    KDWV  L  AL
Sbjct: 1470 AQVSQGLATQLGINWKLHCAYRPQSSGQVERMNRTIKETLTKLALETGGKDWVTLLPLAL 1529

Query: 125  WAYRTAYKTP--IGMSPYGIVF 144
               R    TP   G++PY I++
Sbjct: 1530 LRAR---NTPGRFGLTPYEILY 1548

BLAST of CSPI04G08400 vs. ExPASy Swiss-Prot
Match: Q9TTC1 (Gag-Pol polyprotein OS=Koala retrovirus OX=394239 GN=pro-pol PE=3 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-11
Identity = 49/134 (36.57%), Postives = 67/134 (50.00%), Query Frame = 0

Query: 12   YILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFVNHIITKV 71
            Y+L+  D  S WVEA        +TV + + + IL  FG P+ + SD G  FV  +   +
Sbjct: 1418 YLLVFIDTFSGWVEAFPTKTETALTVCKKILEEILPRFGIPKVLGSDNGPAFVAQVSQGL 1477

Query: 72   LAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKL-VNHSCKDWVDHLDSALWAYRTAY 131
              +  I  K+  AY PQ + Q E  NR IK+ L KL +    KDWV  L  AL   R   
Sbjct: 1478 ATQLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLALETGGKDWVTLLPLALLRAR--- 1537

Query: 132  KTP--IGMSPYGIV 143
             TP   G++PY I+
Sbjct: 1538 NTPGQFGLTPYEIL 1548

BLAST of CSPI04G08400 vs. ExPASy Swiss-Prot
Match: O92815 (Gag-Pol polyprotein OS=Walleye dermal sarcoma virus OX=39720 GN=gag-pol PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 2.4e-11
Identity = 54/188 (28.72%), Postives = 87/188 (46.28%), Query Frame = 0

Query: 8    KNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFVNHI 67
            K  +Y L+  D  SKW E I C K D  TV   L K+I+  +G P  I SD+G HF   I
Sbjct: 1500 KKPMYALVIIDVFSKWPEIIPCNKEDAKTVCDILMKDIIPRWGLPDQIDSDQGTHFTAKI 1559

Query: 68   ITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIK-KILEKLVNHSCKDWVDHLDSALWAY 127
              ++     +  K+    HP+ +   E +NR +K KI++         W + L   L   
Sbjct: 1560 SQELTHSIGVAWKLHCPGHPRSSGIVERTNRTLKSKIIKAQEQLQLSKWTEVLPYVLLEM 1619

Query: 128  RTAYKTPIGMSPYGIVFRKACHLPLELKHKASYSEEAQKPNLTARDSILE-VNQWMRQEE 187
            R   K   G+SP+ IV  +    P++  + +  S       L A D+++  +N+  RQ  
Sbjct: 1620 RATPKKH-GLSPHEIVMGR----PMKTTYLSDMSP------LWATDTLVTYMNKLTRQLS 1676

Query: 188  KFYTELSD 194
             ++ ++ D
Sbjct: 1680 AYHQQVVD 1676

BLAST of CSPI04G08400 vs. ExPASy Swiss-Prot
Match: P03360 (Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=pol PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 5.4e-11
Identity = 46/134 (34.33%), Postives = 66/134 (49.25%), Query Frame = 0

Query: 12   YILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHFVNHIITKV 71
            Y+L+  D  S WVEA    +     V + L  +I+  FG P  I SD G  FV  +  ++
Sbjct: 887  YLLVLVDTFSGWVEAYPAKRETSQVVIKHLIHDIIPRFGLPVQIGSDNGPAFVAKVTQQL 946

Query: 72   LAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLDSALWAYRTAYK 131
                N+  K+  AY PQ + Q E  NR +K+ + KL   +  DWV  L  AL   R    
Sbjct: 947  CEALNVSWKLHCAYRPQSSGQVERMNRTLKETIAKLRIETGGDWVSLLPQALLRARC--- 1006

Query: 132  TP--IGMSPYGIVF 144
            TP   G+SP+ I++
Sbjct: 1007 TPGREGLSPFEILY 1017

BLAST of CSPI04G08400 vs. ExPASy TrEMBL
Match: A0A0A0KXQ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G091900 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 8.8e-97
Identity = 177/183 (96.72%), Postives = 179/183 (97.81%), Query Frame = 0

Query: 255 MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD 314
           MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD
Sbjct: 1   MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD 60

Query: 315 PIKAFKWKKFFIVEGDKVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI 374
           PIKAFKWKKFFIVEG+KVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI
Sbjct: 61  PIKAFKWKKFFIVEGEKVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI 120

Query: 375 VSIEHELVLYYILMKQPFNLRSIINGALLVWRRNPKGAKPFPSTMEKLCLKYLPTLARYH 434
           V IEHELVLYYILMKQPFNLRSIINGAL VWRRNPKGAKPFPSTMEKLCLKYLPTLARY 
Sbjct: 121 VPIEHELVLYYILMKQPFNLRSIINGALFVWRRNPKGAKPFPSTMEKLCLKYLPTLARYP 180

Query: 435 KLP 438
           + P
Sbjct: 181 QTP 183

BLAST of CSPI04G08400 vs. ExPASy TrEMBL
Match: A0A151QL68 (Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-53
Identity = 101/160 (63.12%), Postives = 122/160 (76.25%), Query Frame = 0

Query: 1    MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
            M PFP S    YILLA DYVSKW+EA+   K+D  TV++F+K NIL  FG PRAIISD+G
Sbjct: 1249 MGPFPPSNGFTYILLAVDYVSKWIEAVPTRKDDAQTVAKFVKSNILCRFGIPRAIISDQG 1308

Query: 61   LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
             HF N +   +LAK+ +RHK+   YHPQ N QAEVSNRE+KKILE++V  S KDW   L+
Sbjct: 1309 THFCNRLFNSLLAKHGVRHKVSTPYHPQTNGQAEVSNREVKKILERVVQPSRKDWSSRLE 1368

Query: 121  SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYS 161
             ALWAYRTAYKTPIGMSPY +VF KACHLP+EL+HKA ++
Sbjct: 1369 EALWAYRTAYKTPIGMSPYRLVFGKACHLPVELEHKAYWA 1408

BLAST of CSPI04G08400 vs. ExPASy TrEMBL
Match: A0A803R2M6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 2.3e-52
Identity = 104/160 (65.00%), Postives = 122/160 (76.25%), Query Frame = 0

Query: 1   MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
           M PFP S +++YILLA DYVSKWVEA +   ND  TV RFL+KNI T FGTPRAIISDEG
Sbjct: 287 MGPFPSSFSNLYILLAVDYVSKWVEAAATPANDGKTVLRFLQKNIFTRFGTPRAIISDEG 346

Query: 61  LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
            HF N     +L++Y +RH+  + YHPQ N QAE+SNREIK ILEK V  S KDW   LD
Sbjct: 347 SHFCNKQFEALLSRYGVRHRTALPYHPQSNGQAEISNREIKMILEKTVQRSRKDWSRKLD 406

Query: 121 SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYS 161
            ALWAYRTA+KTPIGMSPY +VF KACHLP+EL+HKA ++
Sbjct: 407 DALWAYRTAFKTPIGMSPYRLVFGKACHLPVELEHKAYWA 446

BLAST of CSPI04G08400 vs. ExPASy TrEMBL
Match: A0A5A7U2P4 (Integrase catalytic domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00730 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.0e-52
Identity = 108/154 (70.13%), Postives = 121/154 (78.57%), Query Frame = 0

Query: 3   PFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLH 62
           PF QS  H+YILL  DYVSKWVEAIS VK DV+TVS+FLKKNI + FGTPRA+I+DEG H
Sbjct: 523 PFSQSGGHLYILLDMDYVSKWVEAISNVKIDVITVSKFLKKNIFSRFGTPRALINDEGSH 582

Query: 63  FVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLDSA 122
           F+NHIITK+L  YNI HK+  A  PQ N Q +V NREI KILEK+VN S KD  DHLDS 
Sbjct: 583 FINHIITKLLVMYNINHKVATANKPQTNGQVDVCNREI-KILEKVVNSSLKDLADHLDST 642

Query: 123 LWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHK 157
           L AY TAYKTPIGMSPY +VF KACHLP EL+ K
Sbjct: 643 LLAYCTAYKTPIGMSPYALVFGKACHLPRELECK 675

BLAST of CSPI04G08400 vs. ExPASy TrEMBL
Match: A0A1U7Y2Z2 (uncharacterized protein LOC104240470 OS=Nicotiana sylvestris OX=4096 GN=LOC104240470 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.9e-52
Identity = 111/230 (48.26%), Postives = 148/230 (64.35%), Query Frame = 0

Query: 1   MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
           M PFP S+ + YILLA DYVSKWVEAI+   ND + V+ F+KKNI + FGTPRA+ISDEG
Sbjct: 291 MGPFPPSRGNKYILLAVDYVSKWVEAIALPTNDAMVVATFVKKNIFSRFGTPRALISDEG 350

Query: 61  LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
            HF N ++  +L KY +RH++  AYHPQ + QA VSNREIKKILEK V+ + K W   LD
Sbjct: 351 THFGNRLLNNLLDKYGVRHRVATAYHPQTSGQANVSNREIKKILEKTVSVNRKGWAAKLD 410

Query: 121 SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYSEEAQKPNLTA--RDSILEVN 180
            ALWAYRTAYK PIG SPY +V+ KACHLP+EL+HKA ++ +    N+ A     ++++N
Sbjct: 411 DALWAYRTAYKMPIGASPYKLVYGKACHLPVELEHKAYWAIKMLNMNIEAACEKRLMQLN 470

Query: 181 QWMRQEEKFYTELSDLGTGVETTRLAGNSSCYTHLKHGAGKVLVDCKEIR 229
           +      K  +  S L    E  R+    +      +G  K LV+   ++
Sbjct: 471 ELWLFPGKLKSRCSGL---FEVVRVMPYGAIELRALNGERKFLVNGPRVK 517

BLAST of CSPI04G08400 vs. NCBI nr
Match: KGN53624.1 (hypothetical protein Csa_015395 [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 1.8e-96
Identity = 177/183 (96.72%), Postives = 179/183 (97.81%), Query Frame = 0

Query: 255 MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD 314
           MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD
Sbjct: 1   MTLDSQAPNVRKKVKVKRERPDLGMLKKGKMVGTPELDKLIKIEKGLLPFKGPLPDFLPD 60

Query: 315 PIKAFKWKKFFIVEGDKVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI 374
           PIKAFKWKKFFIVEG+KVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI
Sbjct: 61  PIKAFKWKKFFIVEGEKVPSTVKAISKLYDLPNGSYAYPDQRIIDNPMRSDMLPTRHDSI 120

Query: 375 VSIEHELVLYYILMKQPFNLRSIINGALLVWRRNPKGAKPFPSTMEKLCLKYLPTLARYH 434
           V IEHELVLYYILMKQPFNLRSIINGAL VWRRNPKGAKPFPSTMEKLCLKYLPTLARY 
Sbjct: 121 VPIEHELVLYYILMKQPFNLRSIINGALFVWRRNPKGAKPFPSTMEKLCLKYLPTLARYP 180

Query: 435 KLP 438
           + P
Sbjct: 181 QTP 183

BLAST of CSPI04G08400 vs. NCBI nr
Match: XP_038887969.1 (uncharacterized protein LOC120077927 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 8.3e-57
Identity = 113/188 (60.11%), Postives = 137/188 (72.87%), Query Frame = 0

Query: 4   FPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEGLHF 63
           FP S  H YILL  DYVSKWV+AISC  ND  TVS+FL+KNI T FGT  A ISDEG HF
Sbjct: 21  FPSSNGHNYILLVVDYVSKWVKAISCASNDAFTVSKFLQKNIFTRFGTLHATISDEGTHF 80

Query: 64  VNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLDSAL 123
           +N I++K+L KYN+ HKI   YHPQ N +AEVSNREIK +LEK+VN + KDW    D AL
Sbjct: 81  INRIVSKILIKYNVHHKIATVYHPQTNGRAEVSNREIKTVLEKVVNLTRKDWAQRQDEAL 140

Query: 124 WAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYSEEAQKPNL-TARDS----ILEVN 183
           WAY T YKTPIGMSPY +VFRKACHLPLEL+HKA ++ +    +L +A D+    +LE++
Sbjct: 141 WAYHTTYKTPIGMSPYALVFRKACHLPLELEHKAFWAIKNLNFDLKSAGDARKLELLELD 200

Query: 184 QWMRQEEK 187
           +W  Q  K
Sbjct: 201 EWRLQSYK 208

BLAST of CSPI04G08400 vs. NCBI nr
Match: XP_038889328.1 (uncharacterized protein K02A2.6-like [Benincasa hispida])

HSP 1 Score: 229.6 bits (584), Expect = 5.3e-56
Identity = 116/198 (58.59%), Postives = 138/198 (69.70%), Query Frame = 0

Query: 1   MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
           M PFP S    YI LA DYVSKWVE ++C +ND  TVS+FL +NI THFGT RA++SDEG
Sbjct: 20  MGPFPLSCGQQYIQLAVDYVSKWVEVVACARNDASTVSKFLTRNIFTHFGTLRALVSDEG 79

Query: 61  LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
            HF+N II+K LAKYN+RH I  AYHPQ N QAEVSNREIK ILEK+VN S K+    LD
Sbjct: 80  THFINRIISKFLAKYNVRHNIATAYHPQTNGQAEVSNREIKSILEKVVNVSRKELTLRLD 139

Query: 121 SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYSEEAQKPNLTA-----RDSIL 180
             LWAYRTAYKTPI MSPY ++F KACHLPLELKHKA ++ +    NL A     +  + 
Sbjct: 140 ETLWAYRTAYKTPICMSPYSLIFGKACHLPLELKHKAFWALKKLNLNLDAAGDQRKLQLN 199

Query: 181 EVNQW---MRQEEKFYTE 191
           E+ +W     +  K Y E
Sbjct: 200 ELEEWWLNTYENNKLYKE 217

BLAST of CSPI04G08400 vs. NCBI nr
Match: WP_217833161.1 (DDE-type integrase/transposase/recombinase, partial [Synechococcus sp. PCC 7002])

HSP 1 Score: 229.2 bits (583), Expect = 7.0e-56
Identity = 105/160 (65.62%), Postives = 127/160 (79.38%), Query Frame = 0

Query: 1   MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
           M PFP S  + YIL+A DYVSKWVEA +C KND  TVS+FLKK I + FGTPRAIISDEG
Sbjct: 168 MGPFPPSCGNQYILVAVDYVSKWVEAAACAKNDANTVSKFLKKQIFSRFGTPRAIISDEG 227

Query: 61  LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
            HF+N IIT +L K+N+ H++  AYHPQ N QAE++N+EIK ILEK+V+ S KDW + LD
Sbjct: 228 THFINRIITNLLTKFNVSHRVATAYHPQTNDQAEITNQEIKSILEKVVSTSRKDWTERLD 287

Query: 121 SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYS 161
            ALWAYRT +KTPIGMSPY +VF KACHL LEL+HKA ++
Sbjct: 288 EALWAYRTTFKTPIGMSPYALVFGKACHLSLELEHKAIWA 327

BLAST of CSPI04G08400 vs. NCBI nr
Match: XP_030479372.1 (uncharacterized protein LOC115696618 [Cannabis sativa])

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-55
Identity = 105/160 (65.62%), Postives = 129/160 (80.62%), Query Frame = 0

Query: 1   MRPFPQSKNHVYILLAEDYVSKWVEAISCVKNDVVTVSRFLKKNILTHFGTPRAIISDEG 60
           M PFPQS  ++YIL+A DYVSKWVEAI+  KND   V +FL K++ T FGTPRA+ISDEG
Sbjct: 194 MGPFPQSFGNLYILVAVDYVSKWVEAIASPKNDARVVMKFLHKHVFTRFGTPRALISDEG 253

Query: 61  LHFVNHIITKVLAKYNIRHKIDIAYHPQINSQAEVSNREIKKILEKLVNHSCKDWVDHLD 120
            HFVN ++  +LAKY+++HKI  AYHPQ N QAE+SNREIK ILEK+VN + KDW   LD
Sbjct: 254 THFVNKVLAALLAKYSVKHKIATAYHPQTNGQAEISNREIKGILEKVVNPNRKDWSQRLD 313

Query: 121 SALWAYRTAYKTPIGMSPYGIVFRKACHLPLELKHKASYS 161
            ALWAYRTAYKTP+GMSPY +V+ KACHLP+EL+HKA ++
Sbjct: 314 DALWAYRTAYKTPLGMSPYRLVYGKACHLPVELEHKAFWA 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P033592.2e-1236.30Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2[more]
P214146.4e-1234.51Gag-Pol polyprotein OS=Gibbon ape leukemia virus OX=11840 GN=pol PE=3 SV=2[more]
Q9TTC11.1e-1136.57Gag-Pol polyprotein OS=Koala retrovirus OX=394239 GN=pro-pol PE=3 SV=2[more]
O928152.4e-1128.72Gag-Pol polyprotein OS=Walleye dermal sarcoma virus OX=39720 GN=gag-pol PE=1 SV=... [more]
P033605.4e-1134.33Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KXQ28.8e-9796.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G091900 PE=4 SV=1[more]
A0A151QL681.6e-5363.13Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4... [more]
A0A803R2M62.3e-5265.00Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5A7U2P43.0e-5270.13Integrase catalytic domain-containing protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1U7Y2Z23.9e-5248.26uncharacterized protein LOC104240470 OS=Nicotiana sylvestris OX=4096 GN=LOC10424... [more]
Match NameE-valueIdentityDescription
KGN53624.11.8e-9696.72hypothetical protein Csa_015395 [Cucumis sativus][more]
XP_038887969.18.3e-5760.11uncharacterized protein LOC120077927 [Benincasa hispida][more]
XP_038889328.15.3e-5658.59uncharacterized protein K02A2.6-like [Benincasa hispida][more]
WP_217833161.17.0e-5665.63DDE-type integrase/transposase/recombinase, partial [Synechococcus sp. PCC 7002][more]
XP_030479372.11.2e-5565.63uncharacterized protein LOC115696618 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..179
e-value: 8.5E-42
score: 144.7
NoneNo IPR availablePANTHERPTHR46148:SF31PROTEIN NYNRIN-LIKEcoord: 1..169
NoneNo IPR availablePANTHERPTHR46148FAMILY NOT NAMEDcoord: 1..169
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 1..146
score: 14.955422
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 3..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G08400.1CSPI04G08400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding