CSPI04G08390 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G08390
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase
Location	Chr4: 6138905 .. 6140176 (+)
RNA-Seq Expression	CSPI04G08390
Synteny	CSPI04G08390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGATTTCTCTGATTTCTTGGAGAGATCAGTTGAGATATTCATGGATGATTTCTCGATCTTTAGGGAATCTTTCAAAGAATGTCTAAATAGTTTGGAGGAAGTAATGGAGAACTGTGAAGAGACACGACTCTTGTTGAACTGGGAGAAGCATCATTGCATGGTGAAAGAAGGAATTGTGCTTGGACACAAAGTCTCCAATGCAGGATTCGAAGTAGACTCCACAAAAATTGATGTAGTTAGTAAGTTGCCACCACCTTGTGATGTTAAGCCAGTAAGAAGCTTTTTGGGCCACGTTGGGTTTTATAGAAGATTTTTATTTGTGGGTTCTCTCAGATTGCCAAACTATTGAGTAACCTATTATGCATCGATCAACCCTTTATTTTCGATGAAAAGTGTAACCAAATGTTTCATGTTATCAAAGACGGGTTGACCTAAGCACCTATCCTCATAACACAAGATCGGTCACAACCATTTGAACTCATGTGTGACGTAGGTGACGTAGCGATAAGGGCTATATTGGGTCAGAAGAAGAATAAGGTGATTCATCCAATCTACTATGCTAACAAGGTTCTCAATGAAGCACAAGAAAATTATACCACCACCAAGAAGAAATTGTTAGTGGCCGTGTTTACGATGGAAAAATTCAGGAGTTACATCCTTGGCACCAAAGTTACGGTGCATTCTAATCATTCTGCGATCAGATATCTCATGGCAAAAAATGATGTTAAACCGCGGTTAATAAGATCATAGACTGCAAAGGGACTGAGAACCAAGTAGCGGATCATTTATCACATCTTCGGAATGCAGAACTTTAATGCGAAAAGACAAATATTGAAGAAATATTTTTAGATGAGCAGCTCTTTTACGTTGAAATAAAAGAACCTTGGTATGTAGACATAGTCAGCTATCTTGTTTGTAAGGTTTGGCCACAAGATTTCAATAGCCAGAAATGAAAGAAGTTATTTCATGAATATCGCTTTTATAGGTGGGATGAGCCTTTCCTTTACAAATTTGACGCATACCATCTATTAAGACGGTGTGTCTCAAAATATGAAACAACTGACATACTAGCCAAATATCATGAAGCATCATATGGAGGACATGTTGGTGGACAAAAGACAGAGGCAAAATTATTGCAAAGTGGGTATTTCTAGACTGCCATTTTCCAGGACGAGGGAAATTTTGTGGTCAAATGCGACAGGTGTCAGAGGACGGGAAATCTGTGTCGCCACGACGAATTACCTCAGAAACCCATCTTCGAGATTGA

mRNA sequence

ATGGTTGATTTCTCTGATTTCTTGGAGAGATCAGTTGAGATATTCATGGATGATTTCTCGATCTTTAGGGAATCTTTCAAAGAATGTCTAAATAGTTTGGAGGAAGTAATGGAGAACTGTGAAGAGACACGACTCTTGTTGAACTGGGAGAAGCATCATTGCATGGTGAAAGAAGGAATTGTGCTTGGACACAAAGTCTCCAATGCAGGATTCGAAGTAGACTCCACAAAAATTGATGTAGTTAGTAAGTTGCCACCACCTTGTGATGTTAAGCCAGTAAGAAGCTTTTTGGGCCACGTTGGGTTTTATAGAAGATTTTTATTTGTGGGTGACGTAGCGATAAGGGCTATATTGGGTCAGAAGAAGAATAAGGTGATTCATCCAATCTACTATGCTAACAAGGTTCTCAATGAAGCACAAGAAAATTATACCACCACCAAGAAGAAATTGTTAGTGGCCGTGTTTACGATGGAAAAATTCAGGAGTTACATCCTTGGCACCAAAGTTACGGTGCATTCTAATCATTCTGCGATCAGATATCTCATGGCAAAAAATGATGTTAAACCGCGGTGGGATGAGCCTTTCCTTTACAAATTTGACGCATACCATCTATTAAGACGGTGTGTCTCAAAATATGAAACAACTGACATACTAGCCAAATATCATGAAGCATCATATGGAGGACATGTGTCAGAGGACGGGAAATCTGTGTCGCCACGACGAATTACCTCAGAAACCCATCTTCGAGATTGA

Coding sequence (CDS)

ATGGTTGATTTCTCTGATTTCTTGGAGAGATCAGTTGAGATATTCATGGATGATTTCTCGATCTTTAGGGAATCTTTCAAAGAATGTCTAAATAGTTTGGAGGAAGTAATGGAGAACTGTGAAGAGACACGACTCTTGTTGAACTGGGAGAAGCATCATTGCATGGTGAAAGAAGGAATTGTGCTTGGACACAAAGTCTCCAATGCAGGATTCGAAGTAGACTCCACAAAAATTGATGTAGTTAGTAAGTTGCCACCACCTTGTGATGTTAAGCCAGTAAGAAGCTTTTTGGGCCACGTTGGGTTTTATAGAAGATTTTTATTTGTGGGTGACGTAGCGATAAGGGCTATATTGGGTCAGAAGAAGAATAAGGTGATTCATCCAATCTACTATGCTAACAAGGTTCTCAATGAAGCACAAGAAAATTATACCACCACCAAGAAGAAATTGTTAGTGGCCGTGTTTACGATGGAAAAATTCAGGAGTTACATCCTTGGCACCAAAGTTACGGTGCATTCTAATCATTCTGCGATCAGATATCTCATGGCAAAAAATGATGTTAAACCGCGGTGGGATGAGCCTTTCCTTTACAAATTTGACGCATACCATCTATTAAGACGGTGTGTCTCAAAATATGAAACAACTGACATACTAGCCAAATATCATGAAGCATCATATGGAGGACATGTGTCAGAGGACGGGAAATCTGTGTCGCCACGACGAATTACCTCAGAAACCCATCTTCGAGATTGA

Protein sequence

MVDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFLFVGDVAIRAILGQKKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAKNDVKPRWDEPFLYKFDAYHLLRRCVSKYETTDILAKYHEASYGGHVSEDGKSVSPRRITSETHLRD*

Homology

[Close]

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.5e-16
Identity = 60/236 (25.42%), Postives = 97/236 (41.10%), Query Frame = 0


Query: 8   LERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHKVS 67
           L +   +++DD  +F  S  E L SL  V E   +  L L  +K   + +E   LGH ++
Sbjct: 357 LNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLT 416

Query: 68  NAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL-------------------- 127
             G + +  KI+ + K P P   K +++FLG  G+YR+F+                    
Sbjct: 417 PDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKI 476

Query: 128 -------------------------------------FVGDVAIRAILGQKKNKVIHPIY 187
                                                   DVA+ A+L Q      HP+ 
Sbjct: 477 DTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDG----HPLS 536

[Close]

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.0e-14
Identity = 59/231 (25.54%), Postives = 98/231 (42.42%), Query Frame = 0


Query: 8   LERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHKVS 67
           L +   +++DD  IF  S  E LNS++ V     +  L L  +K   + KE   LGH V+
Sbjct: 356 LNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVT 415

Query: 68  NAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL-------------------- 127
             G + +  K+  +   P P   K +R+FLG  G+YR+F+                    
Sbjct: 416 PDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYADIAKPMTSCLKKRTKI 475

Query: 128 --------------------------------FV-----GDVAIRAILGQKKNKVIHPIY 182
                                           FV      ++A+ A+L Q      HPI 
Sbjct: 476 DTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNG----HPIS 535

[Close]

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.8e-12
Identity = 59/238 (24.79%), Postives = 89/238 (37.39%), Query Frame = 0


Query: 2   VDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIV 61
           + FS        ++MDD  +   S K  L +L EV   C E  L L+ EK    + E   
Sbjct: 459 IAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTF 518

Query: 62  LGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL-------------- 121
           LGHK ++ G   D  K DV+   P P D    R F+    +YRRF+              
Sbjct: 519 LGHKCTDKGILPDDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFADYSRHITRLC 578

Query: 122 ---------------FV---------------------------GDVAIRAILGQKKNKV 181
                          F+                              A  A+L Q  N  
Sbjct: 579 KKNVPFEWTDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQNHNGH 638

Query: 182 IHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMA 184
             P+ YA++   + + N +TT+++L    + +  FR YI G   TV ++H  + YL +
Sbjct: 639 QLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFS 696

[Close]

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 29/94 (30.85%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 14  IFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHKVSNAGFEV 73
           +++DD  +F E +     +L  V+ +  +  L +N EK H +  +   LG+ V+  G + 
Sbjct: 279 VYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKA 338

Query: 74  DSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL 108
           D  K+  +S++PPP  VK ++ FLG   +YR+F+
Sbjct: 339 DPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFI 372

[Close]

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 62.8 bits (151), Expect = 6.5e-09
Identity = 61/255 (23.92%), Postives = 100/255 (39.22%), Query Frame = 0


Query: 10  RSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHKVSNA 69
           R V +++DD  IF ES +E    L+ V+E  +   L++  +K     +E   LG+ +   
Sbjct: 741 RFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQ 800

Query: 70  GFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRF------------LFVGDVA---- 129
                  K   +   P P  VK  + FLG + +YRRF            LF+ D +    
Sbjct: 801 KIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQPIQLFICDKSQWTE 860

Query: 130 --------------------------------------IRAILGQ--KKNKVIHPIYYAN 189
                                                 I A+L +   KNK++  + Y +
Sbjct: 861 KQDKAIDKLKDALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVVGYFS 920

Query: 190 KVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAKNDVK---PR 205
           K L  AQ+NY   + +LL  +  +  FR  + G   T+ ++H ++  L  KN+      R
Sbjct: 921 KSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQNKNEPARRVQR 980

[Close]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL
Match: A0A2G9HJB6 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_09711 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 8.4e-68
Identity = 131/248 (52.82%), Postives = 171/248 (68.95%), Query Frame = 0


Query: 1   MVDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGI 60
           M  F+D +E  +E+FMDDFS++  SF ECLN+L  V++ CE+T L+LNWEK H MV+EGI
Sbjct: 622 MAIFTDMVENFLEVFMDDFSVYGNSFDECLNNLSSVLKRCEDTNLVLNWEKCHFMVQEGI 681

Query: 61  VLGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFLFVGDVAIRAILGQ 120
           VLGHKVSN G EVD  K++ + KLPPP  VK VR+FLGH         VGD AI A+LGQ
Sbjct: 682 VLGHKVSNRGIEVDKAKLETIEKLPPPTSVKGVRNFLGH---------VGDFAIGAVLGQ 741

Query: 121 KKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRY 180
           +K+K+   IYYA+K LN+AQ NYTTTKK+LL  VF  +KFRSY++GTKV V+++H+AIRY
Sbjct: 742 RKDKISRSIYYASKTLNDAQLNYTTTKKELLAIVFAFDKFRSYLVGTKVIVYTDHAAIRY 801

Query: 181 LMAKNDVKPR---------------WDEPFLYKFDAYHLLRRCVSKYETTDILAKYHEAS 234
           L+ K D KPR               WD+PFL+K    ++LRRCV + E  D+L + H + 
Sbjct: 802 LIEKKDAKPRLIRWKKFLFDTRRYFWDDPFLFKQGPDYILRRCVPEVEMNDVLEQCHASP 860

[Close]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL
Match: A0A151U533 (Transposon Ty3-I Gag-Pol polyprotein (Fragment) OS=Cajanus cajan OX=3821 GN=KK1_007083 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 9.0e-62
Identity = 138/340 (40.59%), Postives = 177/340 (52.06%), Query Frame = 0


Query: 4   FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
           FSD +E+ +EIFMDDFS+F  SF  CL +L  V+  C ET L+LNWEK H MV EGIVLG
Sbjct: 54  FSDLVEKCIEIFMDDFSVFGASFDTCLENLNIVLRRCIETNLVLNWEKCHFMVTEGIVLG 113

Query: 64  HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
           HK+S  G EVD  K++V+SKLPPP +VK +RSFLGH GFYRRF+                
Sbjct: 114 HKISGKGIEVDPAKVEVISKLPPPANVKGIRSFLGHAGFYRRFIQDFSKIAKPLSNLLVK 173

Query: 124 FVGDVAIRAILGQKKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGT 183
            V D A+ A+LGQ+KNK+ H I+YA+KVLNEAQ NY TT+K+LL  V+ +EKFRSY++G+
Sbjct: 174 DVNDYAVGAVLGQRKNKIFHVIHYASKVLNEAQMNYATTEKELLAIVYALEKFRSYLIGS 233

Query: 184 KVTVHSNHSAIRYLMAKNDVKPR------------------------------------- 234
           K+TV ++H+AI+YL+ K+D KPR                                     
Sbjct: 234 KITVFTDHAAIKYLLTKSDSKPRLIRWILLLQEFDLEIKDKKGCENHIADHLSRLENEEV 293

[Close]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL
Match: A0A151R7X5 (Reverse transcriptase OS=Cajanus cajan OX=3821 GN=KK1_040083 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.6e-58
Identity = 131/336 (38.99%), Postives = 174/336 (51.79%), Query Frame = 0


Query: 4    FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
            FSD +E+ +E+FMDDFS+F  SF  CL++L+ V++ C ET L+LNWEK H MV EGIVLG
Sbjct: 681  FSDLVEKCIEVFMDDFSVFGSSFDTCLDNLDTVLKRCVETNLVLNWEKCHFMVTEGIVLG 740

Query: 64   HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
            HK+S  G EVD  KI+V+ KLPPP +VK ++SFLGH GFYRRF+                
Sbjct: 741  HKISCRGIEVDKAKIEVIEKLPPPVNVKGIKSFLGHAGFYRRFIKDFSKIAKPLSNLLNK 800

Query: 124  FVGDVAIRAILGQKKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGT 183
               D AI A+LGQ+K KV H I+YA+KVLN+ Q NY TT+K+LL  V+ +EKFR+Y++G+
Sbjct: 801  DTPDYAIGAVLGQRKEKVFHVIHYASKVLNDTQANYATTEKELLAIVYALEKFRAYLIGS 860

Query: 184  KVTVHSNHSAIRYLMAKNDVKPR------------------------------------- 230
            K+ V ++H+AI+YL+ K+D KPR                                     
Sbjct: 861  KIIVFTDHAAIKYLLTKSDSKPRLIRWILLLQEFDLEIKDKKGCENNVADHLSRLVNNEV 920

[Close]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL
Match: A0A5N6N9T2 (Reverse transcriptase OS=Mikania micrantha OX=192012 GN=E3N88_22237 PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 8.7e-57
Identity = 116/243 (47.74%), Postives = 150/243 (61.73%), Query Frame = 0


Query: 4    FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
            F D +E S+E+FMDDFS+F  SF  CL++L++++  CEE+ L+LNWEK+H MVKEGIVLG
Sbjct: 1756 FHDMIEESMEVFMDDFSVFGSSFDHCLSNLKKMLARCEESNLVLNWEKYHFMVKEGIVLG 1815

Query: 64   HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
            HK+SNAG EVD  K+D +SKLPPP  VK +RSFLGH GFYRRF+                
Sbjct: 1816 HKISNAGLEVDRAKVDTISKLPPPTSVKAIRSFLGHAGFYRRFIKDFSKIARPMTQLLEK 1875

Query: 124  ---FV-------------------------------------GDVAIRAILGQKKNKVIH 183
               FV                                      D A+  +LGQ+K+K  H
Sbjct: 1876 DVQFVFSDECLKAFNCLKEKLVNAPIMVAPDWTLPFELMCDASDYAVGVVLGQRKDKHFH 1935

Query: 184  PIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAKNDV 191
            PIYYA+K LN+AQENYTTT+KKLL  VF  +KFRSY++ +K  V+++H+A+RYL  K D 
Sbjct: 1936 PIYYASKTLNDAQENYTTTEKKLLAVVFAFDKFRSYLILSKTVVYTDHAALRYLFGKQDA 1995

[Close]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL
Match: A0A2G9HYD8 (Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04775 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 8.7e-57
Identity = 137/385 (35.58%), Postives = 177/385 (45.97%), Query Frame = 0


Query: 1    MVDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGI 60
            M  F+D +E  +E+FMDDFS++ +SF ECLN+L  V++ CE+T L+LNWEK H MV+EGI
Sbjct: 824  MAIFTDMVENCLEVFMDDFSVYGDSFDECLNNLSSVLKRCEDTNLVLNWEKCHFMVQEGI 883

Query: 61   VLGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL------------- 120
            VLGHKVSN G EVD  K++ + KLPPP  VK VRSFLGH GFYRRF+             
Sbjct: 884  VLGHKVSNRGIEVDKAKLETIEKLPPPTSVKGVRSFLGHAGFYRRFIKDFSKISKPLCNL 943

Query: 121  -------------------------------------------FVGDVAIRAILGQKKNK 180
                                                          D AI A+LGQ+K+K
Sbjct: 944  LEKDTPFKFDDACLDAFDDLKGRLISAPIITVPDWSFPFELMCDASDFAIGAVLGQRKDK 1003

Query: 181  VIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAK 234
            +   IYYA+K LN+AQ NYTTT+K+LL  VF  +KFRSY++GTKV V+++H+AIRYL+ K
Sbjct: 1004 IFRSIYYASKTLNDAQLNYTTTEKELLAVVFAFDKFRSYLVGTKVIVYTDHAAIRYLIEK 1063

[Close]

BLAST of CSPI04G08390 vs. NCBI nr
Match: PIN17626.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-67
Identity = 131/248 (52.82%), Postives = 171/248 (68.95%), Query Frame = 0


Query: 1   MVDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGI 60
           M  F+D +E  +E+FMDDFS++  SF ECLN+L  V++ CE+T L+LNWEK H MV+EGI
Sbjct: 622 MAIFTDMVENFLEVFMDDFSVYGNSFDECLNNLSSVLKRCEDTNLVLNWEKCHFMVQEGI 681

Query: 61  VLGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFLFVGDVAIRAILGQ 120
           VLGHKVSN G EVD  K++ + KLPPP  VK VR+FLGH         VGD AI A+LGQ
Sbjct: 682 VLGHKVSNRGIEVDKAKLETIEKLPPPTSVKGVRNFLGH---------VGDFAIGAVLGQ 741

Query: 121 KKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRY 180
           +K+K+   IYYA+K LN+AQ NYTTTKK+LL  VF  +KFRSY++GTKV V+++H+AIRY
Sbjct: 742 RKDKISRSIYYASKTLNDAQLNYTTTKKELLAIVFAFDKFRSYLVGTKVIVYTDHAAIRY 801

Query: 181 LMAKNDVKPR---------------WDEPFLYKFDAYHLLRRCVSKYETTDILAKYHEAS 234
           L+ K D KPR               WD+PFL+K    ++LRRCV + E  D+L + H + 
Sbjct: 802 LIEKKDAKPRLIRWKKFLFDTRRYFWDDPFLFKQGPDYILRRCVPEVEMNDVLEQCHASP 860

[Close]

BLAST of CSPI04G08390 vs. NCBI nr
Match: KYP74405.1 (Transposon Ty3-I Gag-Pol polyprotein, partial [Cajanus cajan])

HSP 1 Score: 246.9 bits (629), Expect = 1.9e-61
Identity = 138/340 (40.59%), Postives = 177/340 (52.06%), Query Frame = 0


Query: 4   FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
           FSD +E+ +EIFMDDFS+F  SF  CL +L  V+  C ET L+LNWEK H MV EGIVLG
Sbjct: 54  FSDLVEKCIEIFMDDFSVFGASFDTCLENLNIVLRRCIETNLVLNWEKCHFMVTEGIVLG 113

Query: 64  HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
           HK+S  G EVD  K++V+SKLPPP +VK +RSFLGH GFYRRF+                
Sbjct: 114 HKISGKGIEVDPAKVEVISKLPPPANVKGIRSFLGHAGFYRRFIQDFSKIAKPLSNLLVK 173

Query: 124 FVGDVAIRAILGQKKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGT 183
            V D A+ A+LGQ+KNK+ H I+YA+KVLNEAQ NY TT+K+LL  V+ +EKFRSY++G+
Sbjct: 174 DVNDYAVGAVLGQRKNKIFHVIHYASKVLNEAQMNYATTEKELLAIVYALEKFRSYLIGS 233

Query: 184 KVTVHSNHSAIRYLMAKNDVKPR------------------------------------- 234
           K+TV ++H+AI+YL+ K+D KPR                                     
Sbjct: 234 KITVFTDHAAIKYLLTKSDSKPRLIRWILLLQEFDLEIKDKKGCENHIADHLSRLENEEV 293

[Close]

BLAST of CSPI04G08390 vs. NCBI nr
Match: KYP38652.1 (Transposon Ty3-I Gag-Pol polyprotein [Cajanus cajan])

HSP 1 Score: 236.1 bits (601), Expect = 3.3e-58
Identity = 131/336 (38.99%), Postives = 174/336 (51.79%), Query Frame = 0


Query: 4    FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
            FSD +E+ +E+FMDDFS+F  SF  CL++L+ V++ C ET L+LNWEK H MV EGIVLG
Sbjct: 681  FSDLVEKCIEVFMDDFSVFGSSFDTCLDNLDTVLKRCVETNLVLNWEKCHFMVTEGIVLG 740

Query: 64   HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
            HK+S  G EVD  KI+V+ KLPPP +VK ++SFLGH GFYRRF+                
Sbjct: 741  HKISCRGIEVDKAKIEVIEKLPPPVNVKGIKSFLGHAGFYRRFIKDFSKIAKPLSNLLNK 800

Query: 124  FVGDVAIRAILGQKKNKVIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGT 183
               D AI A+LGQ+K KV H I+YA+KVLN+ Q NY TT+K+LL  V+ +EKFR+Y++G+
Sbjct: 801  DTPDYAIGAVLGQRKEKVFHVIHYASKVLNDTQANYATTEKELLAIVYALEKFRAYLIGS 860

Query: 184  KVTVHSNHSAIRYLMAKNDVKPR------------------------------------- 230
            K+ V ++H+AI+YL+ K+D KPR                                     
Sbjct: 861  KIIVFTDHAAIKYLLTKSDSKPRLIRWILLLQEFDLEIKDKKGCENNVADHLSRLVNNEV 920

[Close]

BLAST of CSPI04G08390 vs. NCBI nr
Match: XP_040871056.1 (uncharacterized protein LOC121174822 [Glycine max])

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-56
Identity = 138/380 (36.32%), Postives = 179/380 (47.11%), Query Frame = 0


Query: 4    FSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLG 63
            FSD +E+S+E+FMDDFS+F  SF  CL +LE V++ C ET L+LNWEK H MV+EGIVLG
Sbjct: 1056 FSDMVEKSIEVFMDDFSVFGSSFDSCLRNLEMVLQRCVETNLVLNWEKCHFMVREGIVLG 1115

Query: 64   HKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL---------------- 123
            HK+S  G EVD  KI+V+ KLPPP ++K VRSFLGH GFYRRF+                
Sbjct: 1116 HKISARGIEVDRAKIEVIEKLPPPLNIKGVRSFLGHAGFYRRFIKDFSKIARPLSNLLNK 1175

Query: 124  ----------------------------------------FVGDVAIRAILGQKKNKVIH 183
                                                       D AI A+LGQ+ +KV H
Sbjct: 1176 DVAFKFDEECSAAFQTLKDKLTTAPVMIAPDWSKDFELMCDASDYAIGAVLGQRHDKVFH 1235

Query: 184  PIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAKNDV 234
             IYYA++VLNEAQ NY TT+K++L  VF +EKFRSY++G+KVT+ ++H+AI++L+AK D 
Sbjct: 1236 AIYYASRVLNEAQLNYATTEKEMLAVVFALEKFRSYLIGSKVTIFTDHAAIKHLLAKTDS 1295

[Close]

BLAST of CSPI04G08390 vs. NCBI nr
Match: PIN22518.1 (DNA-directed DNA polymerase [Handroanthus impetiginosus])

HSP 1 Score: 230.3 bits (586), Expect = 1.8e-56
Identity = 137/385 (35.58%), Postives = 177/385 (45.97%), Query Frame = 0


Query: 1    MVDFSDFLERSVEIFMDDFSIFRESFKECLNSLEEVMENCEETRLLLNWEKHHCMVKEGI 60
            M  F+D +E  +E+FMDDFS++ +SF ECLN+L  V++ CE+T L+LNWEK H MV+EGI
Sbjct: 824  MAIFTDMVENCLEVFMDDFSVYGDSFDECLNNLSSVLKRCEDTNLVLNWEKCHFMVQEGI 883

Query: 61   VLGHKVSNAGFEVDSTKIDVVSKLPPPCDVKPVRSFLGHVGFYRRFL------------- 120
            VLGHKVSN G EVD  K++ + KLPPP  VK VRSFLGH GFYRRF+             
Sbjct: 884  VLGHKVSNRGIEVDKAKLETIEKLPPPTSVKGVRSFLGHAGFYRRFIKDFSKISKPLCNL 943

Query: 121  -------------------------------------------FVGDVAIRAILGQKKNK 180
                                                          D AI A+LGQ+K+K
Sbjct: 944  LEKDTPFKFDDACLDAFDDLKGRLISAPIITVPDWSFPFELMCDASDFAIGAVLGQRKDK 1003

Query: 181  VIHPIYYANKVLNEAQENYTTTKKKLLVAVFTMEKFRSYILGTKVTVHSNHSAIRYLMAK 234
            +   IYYA+K LN+AQ NYTTT+K+LL  VF  +KFRSY++GTKV V+++H+AIRYL+ K
Sbjct: 1004 IFRSIYYASKTLNDAQLNYTTTEKELLAVVFAFDKFRSYLVGTKVIVYTDHAAIRYLIEK 1063

[Close]

BLAST of CSPI04G08390 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 8.4e-04
Identity = 24/80 (30.00%), Postives = 40/80 (50.00%), Query Frame = 0


Query: 30  LNSLEEVMENCEETRLLLNWEKHHCMVKEGIVLGHK--VSNAGFEVDSTKIDVVSKLPPP 89
           +N L  V++  E+ +   N +K      +   LGH+  +S  G   D  K++ +   P P
Sbjct: 1   MNHLGMVLQIWEQHQFYANRKKCAFGQPQIAYLGHRHIISGEGVSADPAKLEAMVGWPEP 60

Query: 90  CDVKPVRSFLGHVGFYRRFL 108
            +   +R FLG  G+YRRF+
Sbjct: 61  KNTTELRGFLGLTGYYRRFV 80

The following BLAST results are available for this feature:

BLAST of CSPI04G08390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	5.5e-16	25.42	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	1.0e-14	25.54	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
P10394	4.8e-12	24.79	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
Q8I7P9	2.2e-09	30.85	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
Q99315	6.5e-09	23.92	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]

BLAST of CSPI04G08390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2G9HJB6	8.4e-68	52.82	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_09711 PE=...	[more]
A0A151U533	9.0e-62	40.59	Transposon Ty3-I Gag-Pol polyprotein (Fragment) OS=Cajanus cajan OX=3821 GN=KK1_...	[more]
A0A151R7X5	1.6e-58	38.99	Reverse transcriptase OS=Cajanus cajan OX=3821 GN=KK1_040083 PE=4 SV=1	[more]
A0A5N6N9T2	8.7e-57	47.74	Reverse transcriptase OS=Mikania micrantha OX=192012 GN=E3N88_22237 PE=3 SV=1	[more]
A0A2G9HYD8	8.7e-57	35.58	Reverse transcriptase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_04775 PE=...	[more]

BLAST of CSPI04G08390 vs. NCBI nr


Match Name	E-value	Identity	Description
PIN17626.1	1.7e-67	52.82	DNA-directed DNA polymerase [Handroanthus impetiginosus]	[more]
KYP74405.1	1.9e-61	40.59	Transposon Ty3-I Gag-Pol polyprotein, partial [Cajanus cajan]	[more]
KYP38652.1	3.3e-58	38.99	Transposon Ty3-I Gag-Pol polyprotein [Cajanus cajan]	[more]
XP_040871056.1	1.1e-56	36.32	uncharacterized protein LOC121174822 [Glycine max]	[more]
PIN22518.1	1.8e-56	35.58	DNA-directed DNA polymerase [Handroanthus impetiginosus]	[more]

BLAST of CSPI04G08390 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00860.1	8.4e-04	30.00	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 23..43
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..250
None	No IPR available	PANTHER	PTHR24559:SF324	TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEIN	coord: 15..106 coord: 111..205
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 15..106 coord: 111..205
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 2..71 e-value: 2.8E-14 score: 55.1
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 76..161 e-value: 5.9E-7 score: 31.4
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 111..190 e-value: 6.5E-17 score: 61.8
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 4..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G08390.1	CSPI04G08390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044260	cellular macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0016740	transferase activity