CSPI04G07970 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G07970
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	NADH kinase
Location	Chr4: 5760154 .. 5764100 (+)
RNA-Seq Expression	CSPI04G07970
Synteny	CSPI04G07970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCAAAGCCCTAAAACCCTGACCCGACCCGAAAGATCTTGTAATCGGCCGAACGTATCAGAAAGGCCGACGTCGATCTCTGCACCGTCTTCACTTCCGTTCGTCCCATTTCCAAACTTTCTCCTTCGAACCCCATTTTTATGTGCGAGATAATGGCTATGAAGAGATTACTTCTGTTTCTTAAGCCATTTGATGCCAACCCTGTTCTCCATTCTGATGCCTTTTCTCGCGTCACCACCCCACAGGTTAATCCTCATTCGACTTTTATTCTCATCCAAACCTTCTATAGTTGTGTGGAAGTTCATTTATTGATGCTTCTGATACCCATCTTCTTTCTTGATGGGTTTTTGCGTTTACTTTTGATGGTCATTTGCAATTATGTGTGGGAATACTTCGAACTTGCATTGGATTTTTGTTCCTTTTAACTCTACTTCACGTCGGATTTCATAGTGATGCTTTAGATAGTGAAAAGAAAAAACAAGTAATGAAACTTTGAAGTATGGGTTCAAAGTTCCACATAAATTCCAATGCTCATTGGATTAATCAAGGTGCTTGCCTTAATATGGGAAAAATGTACATAGAGTATTATTGACTGTTTTAGTTTTTGTTATCAATTTGTTGTAATTTACCTAACAATAATGTGGATTTATGCATGTATGTATTTTGATTTCATCTATCTCATGTGTAGACATATCTATGGACAATTATTGAGAACTATCATATTTTGAAATTAATTCAAAATTTGATAGAAGATTGATGGCAATGACTAAAATGGGGATTTTCTTCAAATGGGCATTATTAATTTTTGAGGAAATGTGACTAGTGTCTATTTCTGCCTTCTTTGTGAGCATTCAATTCTTCTTTGTTCTGTTCAAGTTTAAAGTTAGACCATTTGGAATTAAATTATATATGTTGAGAGCAGCTAGTTTGGTTGTAAAATTAACAGATTTGTGTGAGGCCGTTCTCTTTGTTTATTTCTTCTTTTGGCTCCCATATCGGAAAATTTTAGTTATTTTTATTATGTTCTTAGTTGATTCATGTTTTGCTTTATGTTGGATGTTTGTGTCATTGCAGATCTTACGCCACCTCGAGAACAGGCAGGAGGTACACAGGGAAGCTATAGATGTTTGTAAGGATATTTTGCAGCAGAAGCATGTGGATTGGGAACCTGTATTACGCAATGATTTATCTGAGCCAATTACCAACGTAGACCTTGTTGTTACAGTTGGTGGTGATGGCACCCTATTGCGTGCCAGCCATTTCTTAGATGAGTCCATTCCTATTCTTGGAGTAAATTCTGATCCTACACAAGTGGATGAGGTAACTGTGTGATTTGATTATGCATTACTTTGAATCCCTTGGAAAGGATTAACTATTGGTGGAGATTTATTTTAGTTAAACCACTCTTTCATTGAAAATGAAATCCCGGTTTCAATTATGTCATTGGCCTTTGAACAACGAGTATGTGGAATCACTATCATTATCAATAATGATATTAAACACCGCATTGGTCGATTTGGTACAACATTCTAGAAAATTTGCATAATGGTGTGATCAAAGTTCTTTTATTAAACATGATACTGTATTATATCGAACTATCCTCCATGTATCATAACTTAAAAGTTCTAAGATGTCAATGAAGTGTTTTAAGGTTGGGGAATAGATAGATACACACATCTTTAAATTTGTAATTTAACCTTTTACAGGGCATTTGGGGCAAGGACTATCTAAAACTACGATAGTGATCTCTTGCTACAATAAATATTGTTTAAAAGTCCCCAATTAACTATAGTAAACAATATTTCAAAATCTCCATTCGCTACCGTTTTACTATTTTACATTAATATTTTTTGTATTATGCTACAGTATTTACTATTTCGTTACCAAACTAAAATAGTTTACTCACATAACACATACTATTATTAACTAGAACTAAAACAATATACACCCCAAAAACAAACTCTTATAACCCAACCATAATAATTCAAGATTATAAAAATTCACTCATTGCCCCAAAGGTGCCCTTATTACTTTATACAGTATTTACCAGATGTTTTCTGGTCTAGGTTGAAGAGTTCAGCAATGAGTTTGATGCTTCAAGAAGCACAGGCCATCTATGTGCTGCAACTGTCAATAACTTTGAGCAAGTAAGTCTGCTTTACCTTGGATTCAAATGGTGTTTGGCTTTAGATTTCAATTTGCCCTCTCTCTAGTATTGACAATGATCTATGTATGTTAGTAATAGCAGGATTAATCTGGTTTTGGGAAACAATATTTAGCTCTAAAGAATTTGTGCCTTCAAATGATTGAGTTGGCCAGTATCTTATTTTGAGTAGTATAAGTTTTGACGCCTATTCTACCTGTTAAATTTGATGTTGCCTTATTCAAAAAATGGTATATCATGTTTGTTAGCAATAGCAACTTCGTTTCAGTGGAACATGTAAAGGATCTTATAAACTTTCCAAGGCACTGTTTGAGGCATTTAGCAAGGGGTTTCATCTTTATATAAATGTGTGTAGGGAAGACCGTTTATAAGCCGAGGGAAAACCAGATAGAGAAAACCATCAAATTAGATTACCAGCGAAAATCAGCAGCGAGGGTAAGAACTAAGAGTTTTGTAATTGTGGGAAGTCTAAAAAAATTGAAAGATTTAAATGATTTTCTTTCTATAAAGTAGCACGGTGGACAACAAGATGAATGAAAGTATAAGAGAACAGGGCTTGGGAAGAGATTACTTAGAGAAAGGAAAGGAGGTGTCTGGGAGAATATTTCTTAATTTATTCATTAAAAAGTTTAGAAAAGGTTAAGATGACTAAGGGGCTACGAAGAATTAAAGTCATGGAGGTAGATTCAAATCCTTGGTCTAGTGGTCGAAAATATAATGCTTTAACCAATTAAGCAAAGCTCGAGCCACATCATGGAGTAAGGATTATGTAAAATATTTGACGAACTGCTGCTGCAACATTAATTGAATAATTATCGCTCAATTTTATGGAAATGATAGAGATTAATAAAAATATCGAATATATTGGTCGTTGATTTAATTTATATTCTATTTGAAGAATATTGTAGCTGTAGTCGGAAGAATGGGATACAAGGAGTGAGTGTAATGGACACCTTAATTTGCTACATAGTTGGATTCTTGTAAAATTCAATCCTATAAGGTCAGATCCTTTTAGTTATCACCGGAAAATACAACTACATACAGGTATTAGACAGCATCCTTAATGGTGAGGCAGTTCCTTCCAAGTTGTCAAGGATATCTTTATCTGTAAATTCGGAGCTGCTCTCAAAATATCCTCTTAATGACGTACTAATTGCACATCCGTGTCCTGCATCAGTCTCTCGATTCTCGTTCAAGTAAGAAATTGTACTCTCGCATTATTCTACATCCATATGTTGGTTCCTCTTTCTACCCTCTTCCTTTTGTTTAAGTTATCTTCATTAATCTCTTCCACTCTGCAGAGTTAGGAACGAGCAGTCATGTTCGCCTTTGTTGAACTGTCGATCGAGTGGTCTAAGAGTTTCGACTGCTGCTGGATCAACAGCTGCAATGCTATCTGCAGGTGGATTTCCTATGCCCATTCTATCACAGAAGCTTCAGTACATGGTACGAGAGCCCATTGCCCCAGGAAAGCTGTACAGTTATTTGCATGGAACAATATCGCCTGATCAATCGATTGAAATGGCATGGCTTTGTAATGAGGGCATGATATATATCGATGGTTCACATGTTTGTCATCCTATCCAATATGGCGACATTGTTGAAATATCCTCAAAGGCTCCGAGTCTCAGAGTTTTCTTGCCCCATCAGATGATGAACACTGTTGGGGCAGAAGAAGCTTCAGAGCAACATACACATTCAAAATTGTAGATAAATGCATATATGCAGCTGCATCGGCAAACATAAGATTTTATTTTGAGTAAGTGATTAAGTATGAAGTTTA

mRNA sequence

GTTCAAAGCCCTAAAACCCTGACCCGACCCGAAAGATCTTGTAATCGGCCGAACGTATCAGAAAGGCCGACGTCGATCTCTGCACCGTCTTCACTTCCGTTCGTCCCATTTCCAAACTTTCTCCTTCGAACCCCATTTTTATGTGCGAGATAATGGCTATGAAGAGATTACTTCTGTTTCTTAAGCCATTTGATGCCAACCCTGTTCTCCATTCTGATGCCTTTTCTCGCGTCACCACCCCACAGATCTTACGCCACCTCGAGAACAGGCAGGAGGTACACAGGGAAGCTATAGATGTTTGTAAGGATATTTTGCAGCAGAAGCATGTGGATTGGGAACCTGTATTACGCAATGATTTATCTGAGCCAATTACCAACGTAGACCTTGTTGTTACAGTTGGTGGTGATGGCACCCTATTGCGTGCCAGCCATTTCTTAGATGAGTCCATTCCTATTCTTGGAGTAAATTCTGATCCTACACAAGTGGATGAGGTTGAAGAGTTCAGCAATGAGTTTGATGCTTCAAGAAGCACAGGCCATCTATGTGCTGCAACTGTCAATAACTTTGAGCAAGTATTAGACAGCATCCTTAATGGTGAGGCAGTTCCTTCCAAGTTGTCAAGGATATCTTTATCTGTAAATTCGGAGCTGCTCTCAAAATATCCTCTTAATGACGTACTAATTGCACATCCGTGTCCTGCATCAGTCTCTCGATTCTCGTTCAAAGTTAGGAACGAGCAGTCATGTTCGCCTTTGTTGAACTGTCGATCGAGTGGTCTAAGAGTTTCGACTGCTGCTGGATCAACAGCTGCAATGCTATCTGCAGGTGGATTTCCTATGCCCATTCTATCACAGAAGCTTCAGTACATGGTACGAGAGCCCATTGCCCCAGGAAAGCTGTACAGTTATTTGCATGGAACAATATCGCCTGATCAATCGATTGAAATGGCATGGCTTTGTAATGAGGGCATGATATATATCGATGGTTCACATGTTTGTCATCCTATCCAATATGGCGACATTGTTGAAATATCCTCAAAGGCTCCGAGTCTCAGAGTTTTCTTGCCCCATCAGATGATGAACACTGTTGGGGCAGAAGAAGCTTCAGAGCAACATACACATTCAAAATTGTAGATAAATGCATATATGCAGCTGCATCGGCAAACATAAGATTTTATTTTGAGTAAGTGATTAAGTATGAAGTTTA

Coding sequence (CDS)

ATGTGCGAGATAATGGCTATGAAGAGATTACTTCTGTTTCTTAAGCCATTTGATGCCAACCCTGTTCTCCATTCTGATGCCTTTTCTCGCGTCACCACCCCACAGATCTTACGCCACCTCGAGAACAGGCAGGAGGTACACAGGGAAGCTATAGATGTTTGTAAGGATATTTTGCAGCAGAAGCATGTGGATTGGGAACCTGTATTACGCAATGATTTATCTGAGCCAATTACCAACGTAGACCTTGTTGTTACAGTTGGTGGTGATGGCACCCTATTGCGTGCCAGCCATTTCTTAGATGAGTCCATTCCTATTCTTGGAGTAAATTCTGATCCTACACAAGTGGATGAGGTTGAAGAGTTCAGCAATGAGTTTGATGCTTCAAGAAGCACAGGCCATCTATGTGCTGCAACTGTCAATAACTTTGAGCAAGTATTAGACAGCATCCTTAATGGTGAGGCAGTTCCTTCCAAGTTGTCAAGGATATCTTTATCTGTAAATTCGGAGCTGCTCTCAAAATATCCTCTTAATGACGTACTAATTGCACATCCGTGTCCTGCATCAGTCTCTCGATTCTCGTTCAAAGTTAGGAACGAGCAGTCATGTTCGCCTTTGTTGAACTGTCGATCGAGTGGTCTAAGAGTTTCGACTGCTGCTGGATCAACAGCTGCAATGCTATCTGCAGGTGGATTTCCTATGCCCATTCTATCACAGAAGCTTCAGTACATGGTACGAGAGCCCATTGCCCCAGGAAAGCTGTACAGTTATTTGCATGGAACAATATCGCCTGATCAATCGATTGAAATGGCATGGCTTTGTAATGAGGGCATGATATATATCGATGGTTCACATGTTTGTCATCCTATCCAATATGGCGACATTGTTGAAATATCCTCAAAGGCTCCGAGTCTCAGAGTTTTCTTGCCCCATCAGATGATGAACACTGTTGGGGCAGAAGAAGCTTCAGAGCAACATACACATTCAAAATTGTAG

Protein sequence

MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQKHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEEFSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVLIAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKLQYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSKAPSLRVFLPHQMMNTVGAEEASEQHTHSKL*

Homology

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BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot
Match: Q500Y9 (NADH kinase OS=Arabidopsis thaliana OX=3702 GN=NADK3 PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.8e-107
Identity = 190/308 (61.69%), Postives = 239/308 (77.60%), Query Frame = 0


Query: 5   MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQKHVD 64
           MA+++LLL LKP D  P L ++  S +  PQ+L++LE+R +VH+ AI  C++IL +K V+
Sbjct: 1   MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE 60

Query: 65  WEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEEFSNE 124
           W+P+ RNDLS PI +VD+V+TVGGDGTLL ASHF+D+S+P+LGVNSDPTQ  EVEE S++
Sbjct: 61  WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ 120

Query: 125 FDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVLIAHP 184
           FDASRSTGHLCAATV NFEQVLD IL G  VP+K+SRISL +NSE L  + LND+LIA P
Sbjct: 121 FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP 180

Query: 185 CPASVSRFSFKVRNEQSCS--PLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKLQY 244
           CPA+VSRFSFK++N+   S    +NCRSSGLR+ TAAGSTAAM SAGGF MP+LS+ LQ+
Sbjct: 181 CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF 240

Query: 245 MVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSKAP 304
           MVREPI+PG   S +H T  PDQ +++ W  + G IYIDG  V H +Q GD +EISS AP
Sbjct: 241 MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP 300

Query: 305 SLRVFLPH 311
            L VFL H
Sbjct: 301 VLNVFLSH 308

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BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot
Match: Q6EQG2 (Probable NADH kinase OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0345700 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.3e-99
Identity = 188/321 (58.57%), Postives = 237/321 (73.83%), Query Frame = 0


Query: 5   MAMKRLLLFLKPFDANP--VLHSDAFS--------RVTTPQILRHLENRQEVHREAIDVC 64
           MA++R+LLF+KPFD  P   L + A S        RV+ P++L +L++R  VH+E I++C
Sbjct: 1   MALRRVLLFVKPFDVYPPRPLAAAASSPPPPPPPLRVSNPKVLNYLDDRCRVHKETINLC 60

Query: 65  KDILQQKHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQ 124
           K +LQ+K +DW  V RND+S PI +VDLV++VGGDGTLLRASHFL+ SIP+LGVNSDPT 
Sbjct: 61  KSVLQRKSIDWISVQRNDMSNPIHDVDLVISVGGDGTLLRASHFLNSSIPVLGVNSDPTC 120

Query: 125 VDEVEEFSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKY 184
            DEV+E ++EFDA RSTGHLCAAT  NFEQ+LD+ L+G   PS+LSRIS+ +N   L  Y
Sbjct: 121 PDEVDELTDEFDARRSTGHLCAATAANFEQILDATLDGSRQPSELSRISVKLNGLQLPTY 180

Query: 185 PLNDVLIAHPCPASVSRFSFKVR-NEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPM 244
            LND+L++HPCPASVSRFSF+ R N    S L+NCRSSGLRV+T AGSTAAMLSAGGF M
Sbjct: 181 ALNDILVSHPCPASVSRFSFRKRSNTGESSHLINCRSSGLRVATPAGSTAAMLSAGGFVM 240

Query: 245 PILSQKLQYMVREPIAPGKL-YSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYG 304
           PI S +LQYM+REPI+P       LHG +   Q I + W   EG +Y DGSHV H IQ+G
Sbjct: 241 PISSHELQYMIREPISPRDADKPLLHGLVKQGQHILVVWYNEEGAVYFDGSHVMHSIQHG 300

Query: 305 DIVEISSKAPSLRVFLPHQMM 314
           D +EISS AP L+V LP  ++
Sbjct: 301 DTLEISSDAPILKVILPENLL 321

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BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot
Match: Q58327 (Bifunctional NADP phosphatase/NAD kinase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0917 PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-13
Identity = 65/224 (29.02%), Postives = 109/224 (48.66%), Query Frame = 0


Query: 41  ENRQEVHREAIDVCKDILQQKHVDW--EPVLRNDLSE---PITNVDLVVTVGGDGTLLRA 100
           E+++E    AI++CK  L+ K++ +  E  LR  +      I+ +  ++ +GGDGT+LRA
Sbjct: 310 EDKEEAINLAIEICK-YLKDKNIPFCVEDFLRERVGGDKFDISAISHIIAIGGDGTILRA 369

Query: 101 SHFLD-ESIPILGVNSDPTQVDEVEEFSNEFDASRSTGHLCAATVNNFEQVLDSILNGEA 160
           S  ++ E+IPI+ VN                      G L     +   +++D ++ GE 
Sbjct: 370 SRLVNGETIPIIAVNMG------------------KVGFLAEFCKDEVFEIIDKVIYGEY 429

Query: 161 VPSKLSRISLSV---NSELLSKYPLNDVLIAHPCPASVSRFSFKVRNEQSCSPLLNCRSS 220
              K S++S  +   N  + +   LN++++    PA +  F   V +    + + N R+ 
Sbjct: 430 EIEKRSKLSCKIIKDNRVIKTPSALNEMVVITKNPAKILEFDVYVND----TLVENVRAD 489

Query: 221 GLRVSTAAGSTAAMLSAGGFPMPILSQKLQYMVREPIAPGKLYS 256
           G+ VST  GSTA  LSAGG   PI+   +   +  PI P KL S
Sbjct: 490 GIIVSTPTGSTAYSLSAGG---PIVEPNVDCFIISPICPFKLSS 507

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BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot
Match: Q661V4 (NAD kinase OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=nadK PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.4e-13
Identity = 69/246 (28.05%), Postives = 113/246 (45.93%), Query Frame = 0


Query: 52  DVCKDILQQKHVDWEPVLRNDLSEPI-----TNVDLVVTVGGDGTLLRASHFLDES---- 111
           D  K  L+ KH  +  V+  D+S+P+      N   ++T+GGDGT+L A + L E+    
Sbjct: 21  DDIKVYLETKH--FVEVVLIDVSKPLLSFPRENFLFLITLGGDGTVLLAVNLLLENKNVD 80

Query: 112 IPILGVNSDPTQVDEVEEFSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRI 171
           IPI+ +N                      G L    + +F++V+D       V +K   +
Sbjct: 81  IPIISINMG------------------KVGFLADIKIEDFKKVIDRFFKNSLVINKKFLL 140

Query: 172 SLSV---NSELLSKYPLNDVLIAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAA 231
            ++V     +L+S+Y LND++I       +     KV +E      L+ +S G+ VST  
Sbjct: 141 HVTVYKHGKDLISRYALNDIIIRSSLLNKMIHVDLKVNSEN----FLSYKSDGIIVSTPT 200

Query: 232 GSTAAMLSAGGFPMPILSQKLQYMVREPIAPGKLY--SYLHGTISP-DQSIEMAWLCNEG 283
           GST    SAGG   PIL  +L+  +  PI+P  +Y  S++   +S    S    +     
Sbjct: 201 GSTGYSFSAGG---PILEAELEGFLLTPISPHSVYNRSFVFSKLSKLSLSFSKEYFIAPA 239

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BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot
Match: A4XKP6 (NAD kinase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=nadK PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.4e-13
Identity = 67/261 (25.67%), Postives = 123/261 (47.13%), Query Frame = 0


Query: 43  RQEVHREAIDVCKDILQQKHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLR-ASHFLDE 102
           ++E+ +E +D    +L+ + +DW  VL N+ ++    V+ ++T+GGDGTLL        E
Sbjct: 10  QKELSKEILDKIVSVLKNEKIDW--VLMNEKNKDSVKVNFLITIGGDGTLLNVVEKVAKE 69

Query: 103 SIPILGVNSDPTQVDEVEEFSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR 162
           ++P+LG+N                      G+L     +N    +  I++ +    +   
Sbjct: 70  NLPVLGINCG------------------RVGYLTEEVADNIHFAIKKIIDNDYFIEERHL 129

Query: 163 ISLSVNSELLSKYPLNDVLIAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGS 222
           +      ++   Y LND+ +A    ++ +     +  ++  +     RS G+ ++TA GS
Sbjct: 130 VEAHFKDKIF--YALNDICLAR---STFNIIDLSLYIDEVFAQ--EYRSDGIIIATATGS 189

Query: 223 TAAMLSAGGFPMPILSQKLQYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYID 282
           TA  LSAGG   PI+  +L  MV  PI P  L S     +  D+ +++    +E ++  D
Sbjct: 190 TAYSLSAGG---PIVEPQLGVMVVTPICPHSL-SSRSLVLGDDRVVKIKSESDEVLVVSD 238

Query: 283 GSHVCHPIQYGDIVE--ISSK 301
           G  V   ++ G+ +E  ISSK
Sbjct: 250 G-RVADTLKKGEYLECKISSK 238

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BLAST of CSPI04G07970 vs. ExPASy TrEMBL
Match: A0A0A0KVK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G083530 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.5e-184
Identity = 325/330 (98.48%), Postives = 327/330 (99.09%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MCE MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ
Sbjct: 1   MCERMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL
Sbjct: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSC PLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 181 IAHPCPASVSRFSFKIRNEQSCLPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYSY+HGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK
Sbjct: 241 QYMVREPIAPGKLYSYMHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPHQMMNTVGAEE SEQHTHSKL
Sbjct: 301 APSLRVFLPHQMMNTVGAEEVSEQHTHSKL 330

[Close]

BLAST of CSPI04G07970 vs. ExPASy TrEMBL
Match: A0A5D3CWJ3 (NADH kinase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G00400 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.2e-176
Identity = 310/330 (93.94%), Postives = 320/330 (96.97%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MC+ MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENR EVHREAI+ CKDILQQ
Sbjct: 18  MCQRMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRHEVHREAINFCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWE V RNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 78  KHVDWESVSRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR+SLSVNSELLSKYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRMSLSVNSELLSKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSCS LLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSCSLLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYS++HGTISPDQSIEMAWLCN+G+IYIDGSHVC+PIQYGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLYSFMHGTISPDQSIEMAWLCNDGVIYIDGSHVCYPIQYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPH MMN +GAEE SEQHTH KL
Sbjct: 318 APSLRVFLPHHMMNMIGAEETSEQHTHPKL 347

[Close]

BLAST of CSPI04G07970 vs. ExPASy TrEMBL
Match: A0A1S3BRL4 (NADH kinase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492940 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.2e-176
Identity = 310/330 (93.94%), Postives = 320/330 (96.97%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MC+ MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENR EVHREAI+ CKDILQQ
Sbjct: 18  MCQRMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRHEVHREAINFCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWE V RNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 78  KHVDWESVSRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR+SLSVNSELLSKYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRMSLSVNSELLSKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSCS LLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSCSLLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYS++HGTISPDQSIEMAWLCN+G+IYIDGSHVC+PIQYGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLYSFMHGTISPDQSIEMAWLCNDGVIYIDGSHVCYPIQYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPH MMN +GAEE SEQHTH KL
Sbjct: 318 APSLRVFLPHHMMNMIGAEETSEQHTHPKL 347

[Close]

BLAST of CSPI04G07970 vs. ExPASy TrEMBL
Match: A0A5A7V757 (NADH kinase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G001980 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.8e-174
Identity = 309/330 (93.64%), Postives = 319/330 (96.67%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MC+ MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENR EVHREAI+ CKDILQQ
Sbjct: 18  MCQRMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRHEVHREAINFCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWE V RNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 78  KHVDWESVSRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR+SLSVNSELLSKYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRMSLSVNSELLSKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQS S LLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSRSLLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYS++HGTISPDQSIEMAWLCN+G+IYIDGSHVC+PIQYGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLYSFMHGTISPDQSIEMAWLCNDGVIYIDGSHVCYPIQYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPH MMN +GAEE SEQHTH KL
Sbjct: 318 APSLRVFLPHHMMNMIGAEETSEQHTHPKL 347

[Close]

BLAST of CSPI04G07970 vs. ExPASy TrEMBL
Match: A0A6J1FT64 (NADH kinase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447911 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.2e-156
Identity = 272/328 (82.93%), Postives = 300/328 (91.46%), Query Frame = 0


Query: 2   CEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQK 61
           CE MAM+RLLLFLKPFDANPVLHSD FSRVT+PQILRHLENR  VHREAI  CKD+LQQK
Sbjct: 19  CEKMAMRRLLLFLKPFDANPVLHSDGFSRVTSPQILRHLENRHAVHREAIKFCKDVLQQK 78

Query: 62  HVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEEF 121
            VDWE V RNDLSEPI+NVDLVVTVGGDGTLL+ASH LDESIP+LGVNSDPTQVDEVEEF
Sbjct: 79  PVDWEAVFRNDLSEPISNVDLVVTVGGDGTLLQASHLLDESIPVLGVNSDPTQVDEVEEF 138

Query: 122 SNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVLI 181
            N+FDASRSTGHLCAATVNNFEQVLDS+L+G A PSKLSR+S+SVNSELL+KYPLND+LI
Sbjct: 139 GNQFDASRSTGHLCAATVNNFEQVLDSVLSGVASPSKLSRLSISVNSELLTKYPLNDILI 198

Query: 182 AHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKLQ 241
           AHPCPASVSRFSFK+RNEQ CSPLLNCRSSGLR+STAAGSTAAM SAGGFPMPILS+ LQ
Sbjct: 199 AHPCPASVSRFSFKIRNEQPCSPLLNCRSSGLRISTAAGSTAAMRSAGGFPMPILSRDLQ 258

Query: 242 YMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSKA 301
           YMVREP+ PGKL +++HG +  DQSIEMAWLCN+G+IYIDGSHVCHPIQYGD++EISSKA
Sbjct: 259 YMVREPMTPGKLSNFMHGMVLSDQSIEMAWLCNDGVIYIDGSHVCHPIQYGDVIEISSKA 318

Query: 302 PSLRVFLPHQMMNTVGAEEASEQHTHSK 330
           PSL+VFLPH M+ T   EE SEQHT+ K
Sbjct: 319 PSLKVFLPHHMLYT-AREETSEQHTYPK 345

[Close]

BLAST of CSPI04G07970 vs. NCBI nr
Match: XP_004137227.1 (NADH kinase [Cucumis sativus] >KGN53578.1 hypothetical protein Csa_014537 [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 5.1e-184
Identity = 325/330 (98.48%), Postives = 327/330 (99.09%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MCE MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ
Sbjct: 1   MCERMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL
Sbjct: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSC PLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 181 IAHPCPASVSRFSFKIRNEQSCLPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYSY+HGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK
Sbjct: 241 QYMVREPIAPGKLYSYMHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPHQMMNTVGAEE SEQHTHSKL
Sbjct: 301 APSLRVFLPHQMMNTVGAEEVSEQHTHSKL 330

[Close]

BLAST of CSPI04G07970 vs. NCBI nr
Match: XP_008451739.1 (PREDICTED: NADH kinase isoform X1 [Cucumis melo] >TYK16271.1 NADH kinase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 626.3 bits (1614), Expect = 1.5e-175
Identity = 310/330 (93.94%), Postives = 320/330 (96.97%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MC+ MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENR EVHREAI+ CKDILQQ
Sbjct: 18  MCQRMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRHEVHREAINFCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWE V RNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 78  KHVDWESVSRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR+SLSVNSELLSKYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRMSLSVNSELLSKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSCS LLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSCSLLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYS++HGTISPDQSIEMAWLCN+G+IYIDGSHVC+PIQYGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLYSFMHGTISPDQSIEMAWLCNDGVIYIDGSHVCYPIQYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPH MMN +GAEE SEQHTH KL
Sbjct: 318 APSLRVFLPHHMMNMIGAEETSEQHTHPKL 347

[Close]

BLAST of CSPI04G07970 vs. NCBI nr
Match: KAA0063080.1 (NADH kinase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 621.7 bits (1602), Expect = 3.6e-174
Identity = 309/330 (93.64%), Postives = 319/330 (96.67%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           MC+ MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENR EVHREAI+ CKDILQQ
Sbjct: 18  MCQRMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRHEVHREAINFCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           KHVDWE V RNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE
Sbjct: 78  KHVDWESVSRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSR+SLSVNSELLSKYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRMSLSVNSELLSKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQS S LLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSRSLLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKLYS++HGTISPDQSIEMAWLCN+G+IYIDGSHVC+PIQYGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLYSFMHGTISPDQSIEMAWLCNDGVIYIDGSHVCYPIQYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTHSKL 331
           APSLRVFLPH MMN +GAEE SEQHTH KL
Sbjct: 318 APSLRVFLPHHMMNMIGAEETSEQHTHPKL 347

[Close]

BLAST of CSPI04G07970 vs. NCBI nr
Match: XP_038905969.1 (NADH kinase isoform X1 [Benincasa hispida])

HSP 1 Score: 599.0 bits (1543), Expect = 2.5e-167
Identity = 296/327 (90.52%), Postives = 311/327 (95.11%), Query Frame = 0


Query: 1   MCEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQ 60
           +C  MAMKRLLLFLKPFDANPVLHSD FSRVTTPQILRHLENR EVHREAI+VCKDILQQ
Sbjct: 18  LCNRMAMKRLLLFLKPFDANPVLHSDGFSRVTTPQILRHLENRNEVHREAINVCKDILQQ 77

Query: 61  KHVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 120
           K VDWEPVLRNDLSEPI+NVDLVVTVGGDGTLLRASHFLDESIP+LGVNSDPTQVDEVEE
Sbjct: 78  KDVDWEPVLRNDLSEPISNVDLVVTVGGDGTLLRASHFLDESIPVLGVNSDPTQVDEVEE 137

Query: 121 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVL 180
           FSNEFDASRSTGHLCAATVNNFEQVLDSIL+GEAVPSKLSRISLSVNSELL+KYPLNDVL
Sbjct: 138 FSNEFDASRSTGHLCAATVNNFEQVLDSILSGEAVPSKLSRISLSVNSELLTKYPLNDVL 197

Query: 181 IAHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKL 240
           IAHPCPASVSRFSFK+RNEQSCSPLLNCRSSGLR+STAAGSTAAMLSAGGFPMPILS+KL
Sbjct: 198 IAHPCPASVSRFSFKIRNEQSCSPLLNCRSSGLRISTAAGSTAAMLSAGGFPMPILSRKL 257

Query: 241 QYMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSK 300
           QYMVREPIAPGKL S++H TI PDQSIEMAWLCNEG+IYIDGSH+CHPI+YGDIVEISSK
Sbjct: 258 QYMVREPIAPGKLSSFMHETILPDQSIEMAWLCNEGVIYIDGSHICHPIEYGDIVEISSK 317

Query: 301 APSLRVFLPHQMMNTVGAEEASEQHTH 328
           APSLRVFLPH MM+TV A E  E   H
Sbjct: 318 APSLRVFLPHLMMHTVRAGETGETSEH 344

[Close]

BLAST of CSPI04G07970 vs. NCBI nr
Match: XP_022943059.1 (NADH kinase isoform X1 [Cucurbita moschata])

HSP 1 Score: 561.6 bits (1446), Expect = 4.5e-156
Identity = 272/328 (82.93%), Postives = 300/328 (91.46%), Query Frame = 0


Query: 2   CEIMAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQK 61
           CE MAM+RLLLFLKPFDANPVLHSD FSRVT+PQILRHLENR  VHREAI  CKD+LQQK
Sbjct: 19  CEKMAMRRLLLFLKPFDANPVLHSDGFSRVTSPQILRHLENRHAVHREAIKFCKDVLQQK 78

Query: 62  HVDWEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEEF 121
            VDWE V RNDLSEPI+NVDLVVTVGGDGTLL+ASH LDESIP+LGVNSDPTQVDEVEEF
Sbjct: 79  PVDWEAVFRNDLSEPISNVDLVVTVGGDGTLLQASHLLDESIPVLGVNSDPTQVDEVEEF 138

Query: 122 SNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVLI 181
            N+FDASRSTGHLCAATVNNFEQVLDS+L+G A PSKLSR+S+SVNSELL+KYPLND+LI
Sbjct: 139 GNQFDASRSTGHLCAATVNNFEQVLDSVLSGVASPSKLSRLSISVNSELLTKYPLNDILI 198

Query: 182 AHPCPASVSRFSFKVRNEQSCSPLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKLQ 241
           AHPCPASVSRFSFK+RNEQ CSPLLNCRSSGLR+STAAGSTAAM SAGGFPMPILS+ LQ
Sbjct: 199 AHPCPASVSRFSFKIRNEQPCSPLLNCRSSGLRISTAAGSTAAMRSAGGFPMPILSRDLQ 258

Query: 242 YMVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSKA 301
           YMVREP+ PGKL +++HG +  DQSIEMAWLCN+G+IYIDGSHVCHPIQYGD++EISSKA
Sbjct: 259 YMVREPMTPGKLSNFMHGMVLSDQSIEMAWLCNDGVIYIDGSHVCHPIQYGDVIEISSKA 318

Query: 302 PSLRVFLPHQMMNTVGAEEASEQHTHSK 330
           PSL+VFLPH M+ T   EE SEQHT+ K
Sbjct: 319 PSLKVFLPHHMLYT-AREETSEQHTYPK 345

[Close]

BLAST of CSPI04G07970 vs. TAIR 10
Match: AT1G78590.1 (NAD(H) kinase 3 )

HSP 1 Score: 390.6 bits (1002), Expect = 1.3e-108
Identity = 190/308 (61.69%), Postives = 239/308 (77.60%), Query Frame = 0


Query: 5   MAMKRLLLFLKPFDANPVLHSDAFSRVTTPQILRHLENRQEVHREAIDVCKDILQQKHVD 64
           MA+++LLL LKP D  P L ++  S +  PQ+L++LE+R +VH+ AI  C++IL +K V+
Sbjct: 1   MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE 60

Query: 65  WEPVLRNDLSEPITNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEEFSNE 124
           W+P+ RNDLS PI +VD+V+TVGGDGTLL ASHF+D+S+P+LGVNSDPTQ  EVEE S++
Sbjct: 61  WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ 120

Query: 125 FDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLSVNSELLSKYPLNDVLIAHP 184
           FDASRSTGHLCAATV NFEQVLD IL G  VP+K+SRISL +NSE L  + LND+LIA P
Sbjct: 121 FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP 180

Query: 185 CPASVSRFSFKVRNEQSCS--PLLNCRSSGLRVSTAAGSTAAMLSAGGFPMPILSQKLQY 244
           CPA+VSRFSFK++N+   S    +NCRSSGLR+ TAAGSTAAM SAGGF MP+LS+ LQ+
Sbjct: 181 CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF 240

Query: 245 MVREPIAPGKLYSYLHGTISPDQSIEMAWLCNEGMIYIDGSHVCHPIQYGDIVEISSKAP 304
           MVREPI+PG   S +H T  PDQ +++ W  + G IYIDG  V H +Q GD +EISS AP
Sbjct: 241 MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP 300

Query: 305 SLRVFLPH 311
            L VFL H
Sbjct: 301 VLNVFLSH 308

[Close]

BLAST of CSPI04G07970 vs. TAIR 10
Match: AT3G21070.1 (NAD kinase 1 )

HSP 1 Score: 43.5 bits (101), Expect = 3.8e-04
Identity = 50/197 (25.38%), Postives = 84/197 (42.64%), Query Frame = 0


Query: 65  WEPVLRNDLSEPI----TNVDLVVTVGGDGTLLRASHFLDESIPILGVNSDPTQVDEVEE 124
           WE V+  D  + I    T VDL++T+GGDGT+L A+      +P             +  
Sbjct: 269 WEDVMIYDADKEISLLHTKVDLLITLGGDGTVLWAASMFKGPVP------------PIVP 328

Query: 125 FSNEFDASRSTGHLCAATVNNFEQVLDSILNGEAVPSKLSRISLS-VNSELLSKY-PLND 184
           FS       S G +       +   L++IL G    +   R+    +  +   +Y P   
Sbjct: 329 FS-----MGSLGFMTPFHSEQYRDCLEAILKGPISITLRHRLQCHIIRDKATHEYEPEET 388

Query: 185 VLIAHPCPASVSRFSFKVRNEQSC-SPLLNC-RSSGLRVSTAAGSTAAMLSAGGFPMPIL 244
           +L+ +         S+    E  C +  + C +  GL +ST +GSTA  L+AGG    ++
Sbjct: 389 MLVLNEVTIDRGISSYLTNLECYCDNSFVTCVQGDGLILSTTSGSTAYSLAAGG---SMV 445

Query: 245 SQKLQYMVREPIAPGKL 254
             ++  ++  PI P  L
Sbjct: 449 HPQVPGILFTPICPHSL 445

The following BLAST results are available for this feature:

BLAST of CSPI04G07970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q500Y9	1.8e-107	61.69	NADH kinase OS=Arabidopsis thaliana OX=3702 GN=NADK3 PE=1 SV=1	[more]
Q6EQG2	2.3e-99	58.57	Probable NADH kinase OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0345700 PE...	[more]
Q58327	2.6e-13	29.02	Bifunctional NADP phosphatase/NAD kinase OS=Methanocaldococcus jannaschii (strai...	[more]
Q661V4	4.4e-13	28.05	NAD kinase OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=2...	[more]
A4XKP6	4.4e-13	25.67	NAD kinase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903...	[more]

BLAST of CSPI04G07970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVK3	2.5e-184	98.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G083530 PE=3 SV=1	[more]
A0A5D3CWJ3	7.2e-176	93.94	NADH kinase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A1S3BRL4	7.2e-176	93.94	NADH kinase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492940 PE=3 SV=1	[more]
A0A5A7V757	1.8e-174	93.64	NADH kinase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4...	[more]
A0A6J1FT64	2.2e-156	82.93	NADH kinase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447911 PE=3 SV=1	[more]

BLAST of CSPI04G07970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137227.1	5.1e-184	98.48	NADH kinase [Cucumis sativus] >KGN53578.1 hypothetical protein Csa_014537 [Cucum...	[more]
XP_008451739.1	1.5e-175	93.94	PREDICTED: NADH kinase isoform X1 [Cucumis melo] >TYK16271.1 NADH kinase isoform...	[more]
KAA0063080.1	3.6e-174	93.64	NADH kinase isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038905969.1	2.5e-167	90.52	NADH kinase isoform X1 [Benincasa hispida]	[more]
XP_022943059.1	4.5e-156	82.93	NADH kinase isoform X1 [Cucurbita moschata]	[more]

BLAST of CSPI04G07970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78590.1	1.3e-108	61.69	NAD(H) kinase 3	[more]
AT3G21070.1	3.8e-04	25.38	NAD kinase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017437	ATP-NAD kinase, PpnK-type, C-terminal	GENE3D	2.60.200.30		coord: 171..297 e-value: 9.0E-23 score: 82.4
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 43..170 e-value: 6.2E-19 score: 69.8
IPR002504	NAD kinase	PFAM	PF01513	NAD_kinase	coord: 131..299 e-value: 1.6E-5 score: 24.3 coord: 40..110 e-value: 1.6E-9 score: 37.4
None	No IPR available	PANTHER	PTHR20275	NAD KINASE	coord: 7..313
None	No IPR available	PANTHER	PTHR20275:SF28	NADH KINASE	coord: 7..313
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 61..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G07970.1	CSPI04G07970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019674	NAD metabolic process
biological_process	GO:0006741	NADP biosynthetic process
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0003951	NAD+ kinase activity