CSPI04G07270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G07270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionReticulon-like protein
LocationChr4: 5131859 .. 5136689 (-)
RNA-Seq ExpressionCSPI04G07270
SyntenyCSPI04G07270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCAGCTCTCCTTTTTTGTTTCGAAGTCAGCAAAGCGATCGTATTACTTTCCAATACGAAACTAATAGTTCCCCGAAAGGGATTCGCATTTGGCGTCCGTTAAAAGAAATGCAACCTAGAAAAAGTGAAAGATAAAGTGAAGAAAAGGAAAAATTCAATCCGACCCACTTGAAGAACAGAAAAAAAGAGGGAATTTTTCATCTGGGTTGGTGGTGGCCCGCTTATAATTTCAGTATGTCAGCTTCTCCACGCCATTTCTTGTCGATATTTTTGGAATCCCATAACTCTTTCTTTCTCTCCTCGTTTTCTGTTCTTCTACCTCCCGTTTTTGTGTAATTCCGAACGGAAAATTTTCAGAACCCATTTTCATCTGTCTGTTTGCTTTCTTCTTCTTCAATTTCTTACTTGAAGACCCTTTAATTTCCTGCTCCTGGTTTGTTCTCTTCAGTACCATTTTAGATTTTGTTGGTTTCCTGCTTGTTTTGTGTTCACTTTCAGCTGATTTCGTCTTTTAATTTGCTTGCTTGATGCTTAAATGAGAATAAATGCTGCTAATTCTGCTTACCTTTGTGGTAGTTGTAGTTTTGCTCTCTCTTTTTGTTAACTTCTGCTGTTTATCTTACTATTCGACCAATTAATTTCTTGCACATAGCTTCATCTTTCCGTGTACCGTCTGTCATTTGAAGATTCTATTTATTTTTTTTATCTTGTATTACTGCGGTTCAGTTCTTGGTTGAATCTGAAGTTTATAATGCTTAAATGAGGTTGGATTGCTTTGATTTTGACCATGTCTCTTCCTTCTAGGTGGGTTGAAACATCGACTGACTTCTGCTTTTGAGTTGAAAACGTTTTGCTAAAAATCGCGTTCCTTCCTATTTTATGACATTTCATTGTGTTTTGCCTTTCTGGCTTCCTTATGTTTCATGGTCAGTATTGATCAGCATTTTGTTTTGTCTCTAAATTCTTTTGTGCTTTTGAACGTCTGGTGTTTATTCTTCTGAAGCTTTTTCTAAGCTTTAGTTTCGAGTCATTTCTTTTAGGTAACTTTTGCTTTTTGTGAGCGTTTTACATCTATCTTTGCATTCTTTTATTTATTTATTTTTCTCAATGGAAGTTTGGTTATTCATTAGAAGAAAAAGAAAAAAACCTCTTCACGCCCCTTGTTTCCATAAATTTCATACAAGGCTTCTGTCAATCTCTGGTTGGCCTCAAAAATATCAAATTTCTGTCATTTTTTAGCACAATCAAGGTTTGGTAAGTTATAATATAGGTTTTGAAAGCTCTGCGGATTTTCATTATGCCTATGGAATCATACAGCCTTTCGCCTATTTTGGTGATTTCTACCTTATATGACCTCTTGTTGGAGTGCCTTCCACAAAAATATGTTGTGTAATCTTAATACTTTGATGATTAACATGGACGGTAGAAGTTAACTGTAATAATTTTCTGCCATTGGGACTCTTTCCTCCACCCCTGGTCTTCAGAGTTAGTAACCCTTTTGTTGGCTTGATATTTACCCCGTTAATTGAGCAAAAGTTAAATTCTGGAGCTAATTAGACTGGACCCTCTTCTTTGATCAACTTTCTTACTCACTCACACTCTTCAAATCTTATGTCCTCGTGATCAATTGTGAAAATTTCAGTGTTGGCTAAAACTCAGAATTCAGTAAATTTTTGTTTTTTATCTTGTTCTATCAGATTAAGTGGATTTTCCTTCTTTATTGTGCCTGCTTATACACTAAAGACTCCTTCTTCGTTTTCTTTTTCCTTTATGTATCTTTTTCTATACCTTTTAGTACCTGGCCCAACTTGTGTCTTTTCCTTGAATGTGATTCCATTAAACTCTTTCCCACTCTATATGCTCCTGGTACGAAAGATCTCTTGCTATTCTGATAGTTTGTTTCAAATCAATTTATAATTTTGATTTTGTTGTTGTTACCCAACATTTCGACTACATGCTCCCTTTTTTTTGTGGAAAACTAATTCCATTTGATGAAGAGATTTGTATGTGCCAAAATTTGTAATCCCTCTTGGATGACGATCACCGAAATATCCAACCTGTTGCCTCCTTGCCTTGCTCTAAGAATTGTAGAAAGTGATGGCTCTTTTATGTTCTCTGTCACTTGGTTTCCTTCATAAACAAGATTTGGTGCTGAGAAAGCAAATTGAAAATGAAAACCTCAGCGGTATTTGGTGTTGGTTTGGACATGACATGTAATGGAACTAAGGAAATGCATTACCAGAAGGTGGATCTCCAGCCGAAACATCCTCCTAAAATTTGATCCTGGAGCTAAGACCTAGATTTGCTCCATTACCCATATGATGCCTCTCATATATCCTATTGACTATTATTTAAATTTGTTGTATTGTGTTCAACTTTTACTCTTTTACATAAGTATAGTTCGTACTTTGTGCTCGTTATGTTATCATAAATCTACCTTCTTGGTGAAGAACAGCCTGGTCCTTAACTTTAGCTCCCTTGTTGTTATTTCTTTTCTGAAGCACGAGAACGATGTCTGAGAAGATGACTGCTGAGGATTTTATTAATAACATTTCTGAAGTTCTTACTGAACATGGTCAAAAGGGAAAATCAGTTTCATTTTTTGGGGAAGATAGCAAATCAAGTTCAGTGGCTGGTCAGATCAATCGGATATTTGGACGCCAAAAACCTGTTTACAATCTTCTTGGGGGTGGAAAATGTACATCCTTATTTATGTGTTCTTAGTCTTTTTTTCATCTTCTACAATCTTTTTTCACGTCACAGATCATATTACTTCATTTTGATTTCTATGCTGACCAAATATTTGTAGCTGCTGATGTGCTCCTATGGAGGAACAAGAAAATCTCAGCTAGTGTTTTGACTGGTGCAACTATTATATGGGTGCTTTTTGAATGGCTTAATTACCACTTCTTGACTCTTGTTTGCTTCGCTTTGGTTCTTGGTATGTTGGCTCAGTTTATGTGGACAAATGCGTCAGGATTTCTGAACAGGTGATTGAAAGTTGGATGTTTATGCCTTTGTTTTTCATTGACTTTGATGCTCTTTCTGGTTTAGCGTGCCTGTGCATTCTGTAATAGAATTTCATGCACTTGCAGATCATCGTCCAACGTTCCTCGCCTTGTTCTTCCCAAGGAACTATTTGTCAATATTGCCGTTACGTTTGGTGCTGAGGTTAACCGAGCTCTCTGTTATCTCCAAGATATTGCTTGTGAAGGAAATTTGAAACACTTCCTTGTGGTATGCTAACATATTTCATGTACTTGTTTTGCAATTCTGTTGTATTTCTAAATGTCCTTAGAACGACTTGCTCTAGACATTCATATTTGAGTATTTAGGTACAAGATGTTGAAATTTGATAAAAACAAGCATTGATTTTCTCTTATTGACTTTAAGCTCCTAGTTTGCCTTGCCTCTTGACTTAGGCGTTTTTTTTATTATAGCTCCAGGAACACCAAAACATGGGATATTTGAGAAATATGCTGCCTCAGTTTTGCAAGCGTCTGCTATATTGAATGGAGTTTCTTTGATTATAGCTCCTAGGAAACCTGTTAGATTGCTGTTTTTACTTTTTGATTTTCTTGTCGATACTTCTTTAATGTGAATGTATTTCCAGGCCGTAGTGAGCTTATGGGTTGGTGCTGTGATTAGTAGCTGGTGCAATTTCGTAACTGTCTTGTATATTGGTAAGTGTTTCAGATTCTGCTTTTCAAAACATTTACATCTTCAATTTCTTTGATGATATTATTTAGAAACCAGAAAAAAGATTTAGAGTGTGTTTGGATTGCCTTATATATTTGATTCAAATTGTGATGGCTACTTATAACTTCTTAAATTTTTTAAAAAACTTAATGTTGTAGGATCATATGATTGAGTGAAACTATTTTATGCCACATGGAAAGTATGGTACAGTTTACTGTTTAAAGTGTTTGAATTATTTTGCTTCTTTGTGGAAGGGACTTTAGCTATTTCATTTCATCACCTAATATAGGATGAGCTTTTTCCAATTATATGCCACATGGAAAGTATGGTACTCCACAATGATTGACTTCCGTGAGATTGTTGCGCTATGAAATTAGTTGAGAAGAATGTAAACTGTCTAAAAAAGAACAAAAAAGCATAACCGATGAAAGTGCTGAAATTATCTTGCTTTTTAGTCAGCCGCAACGAATCAAAGTCTGGATAGAAACTTTTTGGTCATGGGAGACTACTAACACATTTCAAATATGTTCAGGCTTTGTTGCGGCCCATACGCTTCCAATTCTGTACGAGAAATATGAAGATGAAGTCGACACCTTCGCATATAAGGTGTTCGATCAGCTTTGCGGCCACTACCAAAAGTTCGATTCAAGTGTCCTCAGCAAGATTCCCAGAGGAGCTTTCAAGGGAAAGAAGTATGAATAATAGATCATTTTCAACTGTCAACAATGCCTAAACCAGCCCTAGTTTTTGCATGGAATTGGTTGTATCATAAGATTTGGTAAAATATTATCTACCTCATGGTGAAATCATAGAGAGACCAAGTGGTAGAAATGAATCTTTAGTTTAAATTTGTATTTGCCAATTTGCTGAAATGGGAACCATCTTTAACTGATTCCTGCAGCTATTAACTTCCATTATTTTAACTGCTTCACATTCCTATCCTTTTGACTTCAGCGCATGAACTGTTGTGAACAAGGAAATAGTTTTTCCCAGTTCAACAATTTTTTAGGGTCGAGATTCAATTATCGACCTTTTAGGTGGTAATTAGTACCTTCTCTACTCAGTTATGCATATATATGTATTATGATGCATCTTTTCTTCTTCAAATTTATGATACAAGTTTGCTTCTTCA

mRNA sequence

ACCAGCTCTCCTTTTTTGTTTCGAAGTCAGCAAAGCGATCGTATTACTTTCCAATACGAAACTAATAGTTCCCCGAAAGGGATTCGCATTTGGCGTCCGTTAAAAGAAATGCAACCTAGAAAAAGTGAAAGATAAAGTGAAGAAAAGGAAAAATTCAATCCGACCCACTTGAAGAACAGAAAAAAAGAGGGAATTTTTCATCTGGGTTGGTGGTGGCCCGCTTATAATTTCAGTATGTCAGCTTCTCCACGCCATTTCTTGTCGATATTTTTGGAATCCCATAACTCTTTCTTTCTCTCCTCGTTTTCTGTTCTTCTACCTCCCGTTTTTGTGTAATTCCGAACGGAAAATTTTCAGAACCCATTTTCATCTGTCTGTTTGCTTTCTTCTTCTTCAATTTCTTACTTGAAGACCCTTTAATTTCCTGCTCCTGCACGAGAACGATGTCTGAGAAGATGACTGCTGAGGATTTTATTAATAACATTTCTGAAGTTCTTACTGAACATGGTCAAAAGGGAAAATCAGTTTCATTTTTTGGGGAAGATAGCAAATCAAGTTCAGTGGCTGGTCAGATCAATCGGATATTTGGACGCCAAAAACCTGTTTACAATCTTCTTGGGGGTGGAAAATCTGCTGATGTGCTCCTATGGAGGAACAAGAAAATCTCAGCTAGTGTTTTGACTGGTGCAACTATTATATGGGTGCTTTTTGAATGGCTTAATTACCACTTCTTGACTCTTGTTTGCTTCGCTTTGGTTCTTGGTATGTTGGCTCAGTTTATGTGGACAAATGCGTCAGGATTTCTGAACAGATCATCGTCCAACGTTCCTCGCCTTGTTCTTCCCAAGGAACTATTTGTCAATATTGCCGTTACGTTTGGTGCTGAGGTTAACCGAGCTCTCTGTTATCTCCAAGATATTGCTTGTGAAGGAAATTTGAAACACTTCCTTGTGGCCGTAGTGAGCTTATGGGTTGGTGCTGTGATTAGTAGCTGGTGCAATTTCGTAACTGTCTTGTATATTGGCTTTGTTGCGGCCCATACGCTTCCAATTCTGTACGAGAAATATGAAGATGAAGTCGACACCTTCGCATATAAGGTGTTCGATCAGCTTTGCGGCCACTACCAAAAGTTCGATTCAAGTGTCCTCAGCAAGATTCCCAGAGGAGCTTTCAAGGGAAAGAAGTATGAATAATAGATCATTTTCAACTGTCAACAATGCCTAAACCAGCCCTAGTTTTTGCATGGAATTGGTTGTATCATAAGATTTGGTAAAATATTATCTACCTCATGGTGAAATCATAGAGAGACCAAGTGGTAGAAATGAATCTTTAGTTTAAATTTGTATTTGCCAATTTGCTGAAATGGGAACCATCTTTAACTGATTCCTGCAGCTATTAACTTCCATTATTTTAACTGCTTCACATTCCTATCCTTTTGACTTCAGCGCATGAACTGTTGTGAACAAGGAAATAGTTTTTCCCAGTTCAACAATTTTTTAGGGTCGAGATTCAATTATCGACCTTTTAGGTGGTAATTAGTACCTTCTCTACTCAGTTATGCATATATATGTATTATGATGCATCTTTTCTTCTTCAAATTTATGATACAAGTTTGCTTCTTCA

Coding sequence (CDS)

ATGTCTGAGAAGATGACTGCTGAGGATTTTATTAATAACATTTCTGAAGTTCTTACTGAACATGGTCAAAAGGGAAAATCAGTTTCATTTTTTGGGGAAGATAGCAAATCAAGTTCAGTGGCTGGTCAGATCAATCGGATATTTGGACGCCAAAAACCTGTTTACAATCTTCTTGGGGGTGGAAAATCTGCTGATGTGCTCCTATGGAGGAACAAGAAAATCTCAGCTAGTGTTTTGACTGGTGCAACTATTATATGGGTGCTTTTTGAATGGCTTAATTACCACTTCTTGACTCTTGTTTGCTTCGCTTTGGTTCTTGGTATGTTGGCTCAGTTTATGTGGACAAATGCGTCAGGATTTCTGAACAGATCATCGTCCAACGTTCCTCGCCTTGTTCTTCCCAAGGAACTATTTGTCAATATTGCCGTTACGTTTGGTGCTGAGGTTAACCGAGCTCTCTGTTATCTCCAAGATATTGCTTGTGAAGGAAATTTGAAACACTTCCTTGTGGCCGTAGTGAGCTTATGGGTTGGTGCTGTGATTAGTAGCTGGTGCAATTTCGTAACTGTCTTGTATATTGGCTTTGTTGCGGCCCATACGCTTCCAATTCTGTACGAGAAATATGAAGATGAAGTCGACACCTTCGCATATAAGGTGTTCGATCAGCTTTGCGGCCACTACCAAAAGTTCGATTCAAGTGTCCTCAGCAAGATTCCCAGAGGAGCTTTCAAGGGAAAGAAGTATGAATAA

Protein sequence

MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPRGAFKGKKYE*
Homology
BLAST of CSPI04G07270 vs. ExPASy Swiss-Prot
Match: Q9SS37 (Reticulon-like protein B8 OS=Arabidopsis thaliana OX=3702 GN=RTNLB8 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.3e-83
Identity = 152/249 (61.04%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           M +K   ED I ++ +  TE  QK K  S F E  +  SV+ + NR+F RQKP++++LGG
Sbjct: 1   MPDKNIVEDVIGDLVDNFTETVQKNKHGSSFFE--QEDSVSSRFNRLFDRQKPIHHVLGG 60

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVL GAT IWVLFEW+N+HFL+LVC+AL+LGM+AQF+W+NASGF
Sbjct: 61  GKSADVLLWRNKKISASVLMGATAIWVLFEWINFHFLSLVCYALLLGMIAQFVWSNASGF 120

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRS S VPRLVLPK+ F  + V  G EVNR L +LQD+AC+GNLK FL+AV+ LWV A+
Sbjct: 121 LNRSQSRVPRLVLPKDFFAEVGVAVGKEVNRGLLFLQDLACKGNLKQFLMAVIGLWVAAM 180

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           + S CNF+TVLYIGFV AHT+P+LYE+YEDEVD F   +  +   HY+K D+  LS+IP 
Sbjct: 181 VGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMDSMIMKFHSHYKKLDTGFLSRIPS 240

Query: 241 GAFKGKKYE 250
           G F  KK E
Sbjct: 241 GKFGLKKRE 247

BLAST of CSPI04G07270 vs. ExPASy Swiss-Prot
Match: O82352 (Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.0e-54
Identity = 107/241 (44.40%), Postives = 155/241 (64.32%), Query Frame = 0

Query: 6   TAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSAD 65
           T E  +  ISE +  H     S S + +    S+V  +I R+FGR+KPV+ +LGGGK AD
Sbjct: 11  TEESLMEKISEKIHHHDSSSSSESEYEKPDSPSAVKAKIYRMFGREKPVHKVLGGGKPAD 70

Query: 66  VLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSS 125
           V LWR+KK+S +VL  AT IWVLFE + YH L+L+C   +L +   F+W+NA   +N++S
Sbjct: 71  VFLWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLWSNAHTLINKTS 130

Query: 126 SNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWC 185
             +P + +P+E F+ +A +   E+N+A   L+ IA   +LK FL+ VV LW+ +V+ +W 
Sbjct: 131 PQIPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVVVGLWIISVVGNWF 190

Query: 186 NFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPRGAFKG 245
           NF+T++YI FV  HT+P+LYEK+ED+VD  A K   +L   Y  FD  VLSKIP  + K 
Sbjct: 191 NFLTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDEKVLSKIPIASLKA 250

Query: 246 K 247
           K
Sbjct: 251 K 251

BLAST of CSPI04G07270 vs. ExPASy Swiss-Prot
Match: Q6DBN4 (Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-52
Identity = 104/239 (43.51%), Postives = 152/239 (63.60%), Query Frame = 0

Query: 8   EDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSADVL 67
           E  +  I++ + +H     S S   +    S++  +I R+FGR+KPV+ +LGGG  ADV 
Sbjct: 13  ESLMEKIADKIHDHDSSSSSDSEHEKPESPSALKAKIYRLFGREKPVHKVLGGGLPADVF 72

Query: 68  LWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSSSN 127
           LWR+KK+SASVL  AT IWVLFE + YHFL+LVC  L+  + A F+ +NA  F+N++   
Sbjct: 73  LWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLLSNAHAFMNKTPPK 132

Query: 128 VPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWCNF 187
           +P + + +E F+ I      E+N+A   L+ IA   +LK FL+ V  LW+ +V+ +W NF
Sbjct: 133 IPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVVFGLWIISVVGNWFNF 192

Query: 188 VTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPRGAFKGK 247
           +T++YI FV  HT+P+LYEK+ED+VD  A K   +L  HY  FD  VLSK+P  + K K
Sbjct: 193 LTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDEKVLSKLPVASLKAK 251

BLAST of CSPI04G07270 vs. ExPASy Swiss-Prot
Match: Q9SH59 (Reticulon-like protein B3 OS=Arabidopsis thaliana OX=3702 GN=RTNLB3 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-50
Identity = 100/240 (41.67%), Postives = 151/240 (62.92%), Query Frame = 0

Query: 8   EDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSADVL 67
           E  +  ISE +  H     S S   +D  S+S+  +I R+FGR++P++ L GGGK AD+ 
Sbjct: 9   ESIMEKISEKIHGHDDSSSSSSDSDDDKNSASLKTKIYRLFGREQPLHKLFGGGKPADIF 68

Query: 68  LWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSSSN 127
           LWRNKK+S  VL  AT+ W+LFE L Y+ LTL     +L +   F+W++AS F+++S  +
Sbjct: 69  LWRNKKVSGGVLGAATVSWILFELLEYNLLTLFGHISILALAVLFLWSSASTFIHKSPLH 128

Query: 128 VPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWCNF 187
           +P + +P+++ + +A     E+NR    L+DIA   +LK FL+ +  LWV + + S CNF
Sbjct: 129 IPEVHIPEDVVLQLASGLRIEINRGFTVLRDIASGRDLKKFLLVIAGLWVLSKVGSSCNF 188

Query: 188 VTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFD----SSVLSKIPRGAF 244
           +T++YI  V   T+P+LYEKYED+VD F  K   ++   Y +FD    S V+SKIP+GAF
Sbjct: 189 LTLIYIATVLLFTIPVLYEKYEDKVDDFGEKAMREIKKQYVEFDVKVLSKVMSKIPKGAF 248

BLAST of CSPI04G07270 vs. ExPASy Swiss-Prot
Match: Q9SUR3 (Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 6.4e-49
Identity = 101/256 (39.45%), Postives = 152/256 (59.38%), Query Frame = 0

Query: 3   EKMTAEDFINNISEVLTEHGQKGKSVSFFGED-----------SKSSSVAGQINRIFGRQ 62
           E +  E  ++ ISE +  HG    S S   +D           S SSS+  ++ R+FGR+
Sbjct: 19  EVVERESLMDKISEKI-HHGGDSSSSSSSSDDEDEKKKTKKPSSPSSSMKSKVYRLFGRE 78

Query: 63  KPVYNLLGGGKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQ 122
           +PV+ +LGGGK AD+ +W+NKK+S  VL GAT  WV+FE + YH LTL+C  +++ +   
Sbjct: 79  QPVHKVLGGGKPADIFMWKNKKMSGGVLGGATAAWVVFELMEYHLLTLLCHVMIVVLAVL 138

Query: 123 FMWTNASGFLNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVA 182
           F+W+NA+ F+N+S   +P + +P+E  + +A     E+NR    L++IA   +LK FL+A
Sbjct: 139 FLWSNATMFINKSPPKIPEVHIPEEPILQLASGLRIEINRGFSSLREIASGRDLKKFLIA 198

Query: 183 VVSLWVGAVISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFD 242
           +  LWV +++    NF+T+ YI  V   T+P+ Y+KYED+VD    K   +L   Y   D
Sbjct: 199 IAGLWVLSILGGCFNFLTLAYIALVLLFTVPLAYDKYEDKVDPLGEKAMIELKKQYAVLD 258

Query: 243 SSVLSKIPRGAFKGKK 248
             VLSKIP G  K KK
Sbjct: 259 EKVLSKIPLGPLKNKK 273

BLAST of CSPI04G07270 vs. ExPASy TrEMBL
Match: A0A0A0KXG6 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G063430 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 5.8e-138
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG
Sbjct: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF
Sbjct: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV
Sbjct: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR
Sbjct: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 241 GAFKGKKYE 249

BLAST of CSPI04G07270 vs. ExPASy TrEMBL
Match: A0A1S3BT84 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 4   MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 63

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 64  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 123

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 124 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 183

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 184 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 243

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 244 GAFKGKKYE 252

BLAST of CSPI04G07270 vs. ExPASy TrEMBL
Match: A0A1S4DYY0 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 8   MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 67

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 68  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 127

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 128 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 187

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 188 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 247

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 248 GAFKGKKYE 256

BLAST of CSPI04G07270 vs. ExPASy TrEMBL
Match: A0A1S4DYZ3 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 49  MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 108

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 109 GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 168

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 169 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 228

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 229 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 288

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 289 GAFKGKKYE 297

BLAST of CSPI04G07270 vs. ExPASy TrEMBL
Match: A0A1S4DYY9 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 5.7e-133
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 20  MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 79

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 80  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 139

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 140 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 199

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 200 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 259

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 260 GAFKGKKYE 268

BLAST of CSPI04G07270 vs. NCBI nr
Match: XP_031740165.1 (reticulon-like protein B8 isoform X1 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.2e-137
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG
Sbjct: 10  MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 69

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF
Sbjct: 70  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 129

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV
Sbjct: 130 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 189

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR
Sbjct: 190 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 249

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 250 GAFKGKKYE 258

BLAST of CSPI04G07270 vs. NCBI nr
Match: XP_011653261.1 (reticulon-like protein B8 isoform X2 [Cucumis sativus] >XP_011653262.1 reticulon-like protein B8 isoform X2 [Cucumis sativus] >KGN53509.1 hypothetical protein Csa_015076 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.2e-137
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG
Sbjct: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF
Sbjct: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV
Sbjct: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR
Sbjct: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 241 GAFKGKKYE 249

BLAST of CSPI04G07270 vs. NCBI nr
Match: XP_016901194.1 (PREDICTED: reticulon-like protein B8 isoform X2 [Cucumis melo])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 20  MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 79

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 80  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 139

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 140 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 199

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 200 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 259

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 260 GAFKGKKYE 268

BLAST of CSPI04G07270 vs. NCBI nr
Match: XP_008451843.1 (PREDICTED: reticulon-like protein B8 isoform X4 [Cucumis melo] >XP_008451844.1 PREDICTED: reticulon-like protein B8 isoform X4 [Cucumis melo] >XP_016901197.1 PREDICTED: reticulon-like protein B8 isoform X4 [Cucumis melo])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 4   MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 63

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 64  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 123

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 124 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 183

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 184 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 243

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 244 GAFKGKKYE 252

BLAST of CSPI04G07270 vs. NCBI nr
Match: XP_016901193.1 (PREDICTED: reticulon-like protein B8 isoform X1 [Cucumis melo])

HSP 1 Score: 483.4 bits (1243), Expect = 1.2e-132
Identity = 237/249 (95.18%), Postives = 245/249 (98.39%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           MSEKM AED  NNISEVL EHGQKGKSVSFFGED+K+SSVAGQINRIFGRQKPVYNLLGG
Sbjct: 49  MSEKMIAEDLFNNISEVLAEHGQKGKSVSFFGEDNKASSVAGQINRIFGRQKPVYNLLGG 108

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQF+WTNASGF
Sbjct: 109 GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFIWTNASGF 168

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAV+SLWVGAV
Sbjct: 169 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVLSLWVGAV 228

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           ISSWCNF+TVLY+GFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHY+KFDSSVLSKIP+
Sbjct: 229 ISSWCNFITVLYVGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYRKFDSSVLSKIPK 288

Query: 241 GAFKGKKYE 250
           GAFKGKKYE
Sbjct: 289 GAFKGKKYE 297

BLAST of CSPI04G07270 vs. TAIR 10
Match: AT3G10260.1 (Reticulon family protein )

HSP 1 Score: 310.1 bits (793), Expect = 1.7e-84
Identity = 152/249 (61.04%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           M +K   ED I ++ +  TE  QK K  S F E  +  SV+ + NR+F RQKP++++LGG
Sbjct: 1   MPDKNIVEDVIGDLVDNFTETVQKNKHGSSFFE--QEDSVSSRFNRLFDRQKPIHHVLGG 60

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVL GAT IWVLFEW+N+HFL+LVC+AL+LGM+AQF+W+NASGF
Sbjct: 61  GKSADVLLWRNKKISASVLMGATAIWVLFEWINFHFLSLVCYALLLGMIAQFVWSNASGF 120

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRS S VPRLVLPK+ F  + V  G EVNR L +LQD+AC+GNLK FL+AV+ LWV A+
Sbjct: 121 LNRSQSRVPRLVLPKDFFAEVGVAVGKEVNRGLLFLQDLACKGNLKQFLMAVIGLWVAAM 180

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           + S CNF+TVLYIGFV AHT+P+LYE+YEDEVD F   +  +   HY+K D+  LS+IP 
Sbjct: 181 VGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMDSMIMKFHSHYKKLDTGFLSRIPS 240

Query: 241 GAFKGKKYE 250
           G F  KK E
Sbjct: 241 GKFGLKKRE 247

BLAST of CSPI04G07270 vs. TAIR 10
Match: AT3G10260.2 (Reticulon family protein )

HSP 1 Score: 310.1 bits (793), Expect = 1.7e-84
Identity = 152/249 (61.04%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           M +K   ED I ++ +  TE  QK K  S F E  +  SV+ + NR+F RQKP++++LGG
Sbjct: 1   MPDKNIVEDVIGDLVDNFTETVQKNKHGSSFFE--QEDSVSSRFNRLFDRQKPIHHVLGG 60

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVL GAT IWVLFEW+N+HFL+LVC+AL+LGM+AQF+W+NASGF
Sbjct: 61  GKSADVLLWRNKKISASVLMGATAIWVLFEWINFHFLSLVCYALLLGMIAQFVWSNASGF 120

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRS S VPRLVLPK+ F  + V  G EVNR L +LQD+AC+GNLK FL+AV+ LWV A+
Sbjct: 121 LNRSQSRVPRLVLPKDFFAEVGVAVGKEVNRGLLFLQDLACKGNLKQFLMAVIGLWVAAM 180

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           + S CNF+TVLYIGFV AHT+P+LYE+YEDEVD F   +  +   HY+K D+  LS+IP 
Sbjct: 181 VGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMDSMIMKFHSHYKKLDTGFLSRIPS 240

Query: 241 GAFKGKKYE 250
           G F  KK E
Sbjct: 241 GKFGLKKRE 247

BLAST of CSPI04G07270 vs. TAIR 10
Match: AT3G10260.3 (Reticulon family protein )

HSP 1 Score: 310.1 bits (793), Expect = 1.7e-84
Identity = 152/249 (61.04%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSEKMTAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGG 60
           M +K   ED I ++ +  TE  QK K  S F E  +  SV+ + NR+F RQKP++++LGG
Sbjct: 21  MPDKNIVEDVIGDLVDNFTETVQKNKHGSSFFE--QEDSVSSRFNRLFDRQKPIHHVLGG 80

Query: 61  GKSADVLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGF 120
           GKSADVLLWRNKKISASVL GAT IWVLFEW+N+HFL+LVC+AL+LGM+AQF+W+NASGF
Sbjct: 81  GKSADVLLWRNKKISASVLMGATAIWVLFEWINFHFLSLVCYALLLGMIAQFVWSNASGF 140

Query: 121 LNRSSSNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAV 180
           LNRS S VPRLVLPK+ F  + V  G EVNR L +LQD+AC+GNLK FL+AV+ LWV A+
Sbjct: 141 LNRSQSRVPRLVLPKDFFAEVGVAVGKEVNRGLLFLQDLACKGNLKQFLMAVIGLWVAAM 200

Query: 181 ISSWCNFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPR 240
           + S CNF+TVLYIGFV AHT+P+LYE+YEDEVD F   +  +   HY+K D+  LS+IP 
Sbjct: 201 VGSCCNFLTVLYIGFVGAHTMPVLYERYEDEVDGFMDSMIMKFHSHYKKLDTGFLSRIPS 260

Query: 241 GAFKGKKYE 250
           G F  KK E
Sbjct: 261 GKFGLKKRE 267

BLAST of CSPI04G07270 vs. TAIR 10
Match: AT2G46170.1 (Reticulon family protein )

HSP 1 Score: 213.4 bits (542), Expect = 2.1e-55
Identity = 107/241 (44.40%), Postives = 155/241 (64.32%), Query Frame = 0

Query: 6   TAEDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSAD 65
           T E  +  ISE +  H     S S + +    S+V  +I R+FGR+KPV+ +LGGGK AD
Sbjct: 11  TEESLMEKISEKIHHHDSSSSSESEYEKPDSPSAVKAKIYRMFGREKPVHKVLGGGKPAD 70

Query: 66  VLLWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSS 125
           V LWR+KK+S +VL  AT IWVLFE + YH L+L+C   +L +   F+W+NA   +N++S
Sbjct: 71  VFLWRDKKLSGAVLGVATAIWVLFELVEYHLLSLLCHISILALGGLFLWSNAHTLINKTS 130

Query: 126 SNVPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWC 185
             +P + +P+E F+ +A +   E+N+A   L+ IA   +LK FL+ VV LW+ +V+ +W 
Sbjct: 131 PQIPEIHVPEEAFLVVASSLRNELNQAFVILRSIALGRDLKKFLMVVVGLWIISVVGNWF 190

Query: 186 NFVTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPRGAFKG 245
           NF+T++YI FV  HT+P+LYEK+ED+VD  A K   +L   Y  FD  VLSKIP  + K 
Sbjct: 191 NFLTLVYICFVILHTVPMLYEKHEDKVDPLAEKAMKELQKQYVVFDEKVLSKIPIASLKA 250

Query: 246 K 247
           K
Sbjct: 251 K 251

BLAST of CSPI04G07270 vs. TAIR 10
Match: AT3G61560.1 (Reticulon family protein )

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-53
Identity = 104/239 (43.51%), Postives = 152/239 (63.60%), Query Frame = 0

Query: 8   EDFINNISEVLTEHGQKGKSVSFFGEDSKSSSVAGQINRIFGRQKPVYNLLGGGKSADVL 67
           E  +  I++ + +H     S S   +    S++  +I R+FGR+KPV+ +LGGG  ADV 
Sbjct: 13  ESLMEKIADKIHDHDSSSSSDSEHEKPESPSALKAKIYRLFGREKPVHKVLGGGLPADVF 72

Query: 68  LWRNKKISASVLTGATIIWVLFEWLNYHFLTLVCFALVLGMLAQFMWTNASGFLNRSSSN 127
           LWR+KK+SASVL  AT IWVLFE + YHFL+LVC  L+  + A F+ +NA  F+N++   
Sbjct: 73  LWRDKKLSASVLGVATAIWVLFELVEYHFLSLVCHILIFALAALFLLSNAHAFMNKTPPK 132

Query: 128 VPRLVLPKELFVNIAVTFGAEVNRALCYLQDIACEGNLKHFLVAVVSLWVGAVISSWCNF 187
           +P + + +E F+ I      E+N+A   L+ IA   +LK FL+ V  LW+ +V+ +W NF
Sbjct: 133 IPEIHIKEEHFLMIVSALRNELNQAFVILRSIALGRDLKKFLMVVFGLWIISVVGNWFNF 192

Query: 188 VTVLYIGFVAAHTLPILYEKYEDEVDTFAYKVFDQLCGHYQKFDSSVLSKIPRGAFKGK 247
           +T++YI FV  HT+P+LYEK+ED+VD  A K   +L  HY  FD  VLSK+P  + K K
Sbjct: 193 LTLVYICFVVLHTVPMLYEKHEDKVDPVAEKTLKELKKHYMVFDEKVLSKLPVASLKAK 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SS372.3e-8361.04Reticulon-like protein B8 OS=Arabidopsis thaliana OX=3702 GN=RTNLB8 PE=2 SV=1[more]
O823523.0e-5444.40Reticulon-like protein B5 OS=Arabidopsis thaliana OX=3702 GN=RTNLB5 PE=1 SV=1[more]
Q6DBN42.1e-5243.51Reticulon-like protein B6 OS=Arabidopsis thaliana OX=3702 GN=RTNLB6 PE=1 SV=1[more]
Q9SH591.5e-5041.67Reticulon-like protein B3 OS=Arabidopsis thaliana OX=3702 GN=RTNLB3 PE=2 SV=1[more]
Q9SUR36.4e-4939.45Reticulon-like protein B1 OS=Arabidopsis thaliana OX=3702 GN=RTNLB1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KXG65.8e-138100.00Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G063430 PE=4 SV=1[more]
A0A1S3BT845.7e-13395.18Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1[more]
A0A1S4DYY05.7e-13395.18Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1[more]
A0A1S4DYZ35.7e-13395.18Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1[more]
A0A1S4DYY95.7e-13395.18Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103493007 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_031740165.11.2e-137100.00reticulon-like protein B8 isoform X1 [Cucumis sativus][more]
XP_011653261.11.2e-137100.00reticulon-like protein B8 isoform X2 [Cucumis sativus] >XP_011653262.1 reticulon... [more]
XP_016901194.11.2e-13295.18PREDICTED: reticulon-like protein B8 isoform X2 [Cucumis melo][more]
XP_008451843.11.2e-13295.18PREDICTED: reticulon-like protein B8 isoform X4 [Cucumis melo] >XP_008451844.1 P... [more]
XP_016901193.11.2e-13295.18PREDICTED: reticulon-like protein B8 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G10260.11.7e-8461.04Reticulon family protein [more]
AT3G10260.21.7e-8461.04Reticulon family protein [more]
AT3G10260.31.7e-8461.04Reticulon family protein [more]
AT2G46170.12.1e-5544.40Reticulon family protein [more]
AT3G61560.11.5e-5343.51Reticulon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003388ReticulonPFAMPF02453Reticuloncoord: 64..218
e-value: 7.9E-51
score: 172.1
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 63..249
score: 43.354843
IPR045064Reticulon-like protein, plantPANTHERPTHR10994RETICULONcoord: 1..249
NoneNo IPR availablePANTHERPTHR10994:SF173RETICULON-LIKE PROTEIN B8coord: 1..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G07270.1CSPI04G07270.1mRNA
CSPI04G07270.2CSPI04G07270.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009617 response to bacterium
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane