CSPI04G06970 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G06970
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionnine-cis-epoxycarotenoid dioxygenase 4
LocationChr4: 4917474 .. 4919673 (-)
RNA-Seq ExpressionCSPI04G06970
SyntenyCSPI04G06970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCCATCCTTTAAATTCTATCATCTTTGAAAGAAGTAGAAGGGAGTAGGGACCAACCTATCATCAATTCTTATCTGAAGAAGTTGAAATTTCACTTCCTTTCCCAATCATTTTCTTCCATCAATGGATTCCATTTCTTCCCCTTTCCTCTCCGGCAGAAATCTCATTCTCTCTCCCCCAATTTCCAGTTCCCTCCCACCCATTTCAACCCCCATCTACTCTGTTCTCACCGAACAAAACGTCAAAAAGAATACCCCTCCACCAGATGCCGATTCTCCATCCCCGCCCTTGCCCCGACCATCTCCTCCCTCCCCACCAATGCCCAGGGTCTCTTCTACCAGACGAGTTCAGCCGTCTCTACCTGCGAGATTCTTCAACGCCTTCGATGACTTAATCAATAACTTCATAAACCCCCCGGTCAGTCCCTCAGTGGATCCACGATACATCCTCGCCGACAACTTCGCTCCGGTGGATGAGCTACCACCGACGGAGTGTGAAGTCATCTATGGCTCGTTGCCTTCATCTCTCAACGGTGCGTACATTCGTAACGGACCAAATCCTCAGTACCTTCCTCGTGGTCCCTACCATTTGTTCGACGGCGACGGAATGCTTCACTCTCTTCGAATCTCCGATGGCCGTGCCGTTCTGTGTAGTCGTTATGTCAAAACTTACAAATATACTTTGGAGCGTGACGCTGGCCATCCTGTTTTCCCCAACGTATTTTCCGGCTTTAATGGCCTTACTGCCTCTGCCGCACGAGGCGCCGTCGCCGTTGGCCGGATATTAACCGGGCAGTATAATCCTGCAAACGGCATTGGTCTTGCAAACACTAGTCTGGCTTTCTTCGGTGACCGACTTTACGCCCTAGGTGAGTCTGATTTACCGTATCCGATTCGATTAACTCCAAACGGTGATATTGAAACTCTCGCCCGTCATGACTTCGACGGAAAACTCACTCTTAGCATGACTGCTCATCCGAAAGTCGATTCCGATACCGGTGAGGCATTTGCGTTCCGATACGGTCCTTTGCCTCCGTTTTTAACTTACTTCCGATTCGACAAAAACGGAGCCAAACATTCAGATGTGCCGATACTTTCGATGAATCGACCGTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGACATTCAGATTGGAATTAACCCTACGCAGATGATAATTGAAGGAGGATCTCCAGTGGGATCAGATCCATCGAAAATTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGATGAATCAAAGATGAAGTGGTTCGATGTACCTGGATTGAACTTAATTCACGCCATTAACGCTTGGGATGAAGATGATGCTGTGGTTATAGTGGCGCCAAATATTCTCTCCGTAGAACACGCACTAGAGAGGATGGATCTGGTTCATGCCTTAGTGGAGAAAATCAGAATCGACCTGAAAACAGGAATAGTAACGCGGACGCCATTGTCGACCAGAAACCTAGACTTTGGAGTGATACATCCCTCGTACGTCGGGAAGAAGCACAGGTTCGTTTACGCCGGTGTTGGAGATCCGATGCCCAAGATTTCGGGAGTGGTGAAGCTCGACATATCTCAGGAAGAACGTCGAGACTGCATCGTCGCTTGTAGAATTTTCGGACCTGGTTGCTATGGCGGGGAACCGTTTTTCGTGCCGAGGGAAAGGGAACGTTCCGACGAGACAGAGGCAGAGGAGGACGACGGATATGTCGTCTCATACGTTCATGATGAAAACTCCGGTGAGTCGAGGTTCATTGTCATGGACGCGAAATCGCCGGAGCTGGAAATTATCGCCGCCGTGAAGCTGCCGCGACGCGTCCCTTACGGTTTTCATGGATTGTTCGTGAAAGAAAGCGACCTCAATAAGCTATGAAATGGAAGGTATCTTAATAACTCAACCGTAATTTTAGACCGAACCGGACTTTCGACCAGACCGCTTATAGCCGGTTTAAAAAGTATATATACGCTCTTAAAATTTTAAATAATACTAATAGTAATAGAATGTATTTATAGGTATTCATATATAAAGAAGGAAGTGTAAGTAAATTAACCATTAGAAGCATTGTATTGTATGGGTGCTTATTTTAAAATTTAGAATATTTATCGAAATTACTCCTAAAAAATCCTCATATTTTTACTAGAGTTTTAAATTAATTCGCTTATTAAATGTTTA

mRNA sequence

CTTCCCATCCTTTAAATTCTATCATCTTTGAAAGAAGTAGAAGGGAGTAGGGACCAACCTATCATCAATTCTTATCTGAAGAAGTTGAAATTTCACTTCCTTTCCCAATCATTTTCTTCCATCAATGGATTCCATTTCTTCCCCTTTCCTCTCCGGCAGAAATCTCATTCTCTCTCCCCCAATTTCCAGTTCCCTCCCACCCATTTCAACCCCCATCTACTCTGTTCTCACCGAACAAAACGTCAAAAAGAATACCCCTCCACCAGATGCCGATTCTCCATCCCCGCCCTTGCCCCGACCATCTCCTCCCTCCCCACCAATGCCCAGGGTCTCTTCTACCAGACGAGTTCAGCCGTCTCTACCTGCGAGATTCTTCAACGCCTTCGATGACTTAATCAATAACTTCATAAACCCCCCGGTCAGTCCCTCAGTGGATCCACGATACATCCTCGCCGACAACTTCGCTCCGGTGGATGAGCTACCACCGACGGAGTGTGAAGTCATCTATGGCTCGTTGCCTTCATCTCTCAACGGTGCGTACATTCGTAACGGACCAAATCCTCAGTACCTTCCTCGTGGTCCCTACCATTTGTTCGACGGCGACGGAATGCTTCACTCTCTTCGAATCTCCGATGGCCGTGCCGTTCTGTGTAGTCGTTATGTCAAAACTTACAAATATACTTTGGAGCGTGACGCTGGCCATCCTGTTTTCCCCAACGTATTTTCCGGCTTTAATGGCCTTACTGCCTCTGCCGCACGAGGCGCCGTCGCCGTTGGCCGGATATTAACCGGGCAGTATAATCCTGCAAACGGCATTGGTCTTGCAAACACTAGTCTGGCTTTCTTCGGTGACCGACTTTACGCCCTAGGTGAGTCTGATTTACCGTATCCGATTCGATTAACTCCAAACGGTGATATTGAAACTCTCGCCCGTCATGACTTCGACGGAAAACTCACTCTTAGCATGACTGCTCATCCGAAAGTCGATTCCGATACCGGTGAGGCATTTGCGTTCCGATACGGTCCTTTGCCTCCGTTTTTAACTTACTTCCGATTCGACAAAAACGGAGCCAAACATTCAGATGTGCCGATACTTTCGATGAATCGACCGTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGACATTCAGATTGGAATTAACCCTACGCAGATGATAATTGAAGGAGGATCTCCAGTGGGATCAGATCCATCGAAAATTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGATGAATCAAAGATGAAGTGGTTCGATGTACCTGGATTGAACTTAATTCACGCCATTAACGCTTGGGATGAAGATGATGCTGTGGTTATAGTGGCGCCAAATATTCTCTCCGTAGAACACGCACTAGAGAGGATGGATCTGGTTCATGCCTTAGTGGAGAAAATCAGAATCGACCTGAAAACAGGAATAGTAACGCGGACGCCATTGTCGACCAGAAACCTAGACTTTGGAGTGATACATCCCTCGTACGTCGGGAAGAAGCACAGGTTCGTTTACGCCGGTGTTGGAGATCCGATGCCCAAGATTTCGGGAGTGGTGAAGCTCGACATATCTCAGGAAGAACGTCGAGACTGCATCGTCGCTTGTAGAATTTTCGGACCTGGTTGCTATGGCGGGGAACCGTTTTTCGTGCCGAGGGAAAGGGAACGTTCCGACGAGACAGAGGCAGAGGAGGACGACGGATATGTCGTCTCATACGTTCATGATGAAAACTCCGGTGAGTCGAGGTTCATTGTCATGGACGCGAAATCGCCGGAGCTGGAAATTATCGCCGCCGTGAAGCTGCCGCGACGCGTCCCTTACGGTTTTCATGGATTGTTCGTGAAAGAAAGCGACCTCAATAAGCTATGAAATGGAAGGTATCTTAATAACTCAACCGTAATTTTAGACCGAACCGGACTTTCGACCAGACCGCTTATAGCCGGTTTAAAAAGTATATATACGCTCTTAAAATTTTAAATAATACTAATAGTAATAGAATGTATTTATAGGTATTCATATATAAAGAAGGAAGTGTAAGTAAATTAACCATTAGAAGCATTGTATTGTATGGGTGCTTATTTTAAAATTTAGAATATTTATCGAAATTACTCCTAAAAAATCCTCATATTTTTACTAGAGTTTTAAATTAATTCGCTTATTAAATGTTTA

Coding sequence (CDS)

ATGGATTCCATTTCTTCCCCTTTCCTCTCCGGCAGAAATCTCATTCTCTCTCCCCCAATTTCCAGTTCCCTCCCACCCATTTCAACCCCCATCTACTCTGTTCTCACCGAACAAAACGTCAAAAAGAATACCCCTCCACCAGATGCCGATTCTCCATCCCCGCCCTTGCCCCGACCATCTCCTCCCTCCCCACCAATGCCCAGGGTCTCTTCTACCAGACGAGTTCAGCCGTCTCTACCTGCGAGATTCTTCAACGCCTTCGATGACTTAATCAATAACTTCATAAACCCCCCGGTCAGTCCCTCAGTGGATCCACGATACATCCTCGCCGACAACTTCGCTCCGGTGGATGAGCTACCACCGACGGAGTGTGAAGTCATCTATGGCTCGTTGCCTTCATCTCTCAACGGTGCGTACATTCGTAACGGACCAAATCCTCAGTACCTTCCTCGTGGTCCCTACCATTTGTTCGACGGCGACGGAATGCTTCACTCTCTTCGAATCTCCGATGGCCGTGCCGTTCTGTGTAGTCGTTATGTCAAAACTTACAAATATACTTTGGAGCGTGACGCTGGCCATCCTGTTTTCCCCAACGTATTTTCCGGCTTTAATGGCCTTACTGCCTCTGCCGCACGAGGCGCCGTCGCCGTTGGCCGGATATTAACCGGGCAGTATAATCCTGCAAACGGCATTGGTCTTGCAAACACTAGTCTGGCTTTCTTCGGTGACCGACTTTACGCCCTAGGTGAGTCTGATTTACCGTATCCGATTCGATTAACTCCAAACGGTGATATTGAAACTCTCGCCCGTCATGACTTCGACGGAAAACTCACTCTTAGCATGACTGCTCATCCGAAAGTCGATTCCGATACCGGTGAGGCATTTGCGTTCCGATACGGTCCTTTGCCTCCGTTTTTAACTTACTTCCGATTCGACAAAAACGGAGCCAAACATTCAGATGTGCCGATACTTTCGATGAATCGACCGTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGACATTCAGATTGGAATTAACCCTACGCAGATGATAATTGAAGGAGGATCTCCAGTGGGATCAGATCCATCGAAAATTTCAAGAGTAGGACTAATTCCTCGATACGCTAACGATGAATCAAAGATGAAGTGGTTCGATGTACCTGGATTGAACTTAATTCACGCCATTAACGCTTGGGATGAAGATGATGCTGTGGTTATAGTGGCGCCAAATATTCTCTCCGTAGAACACGCACTAGAGAGGATGGATCTGGTTCATGCCTTAGTGGAGAAAATCAGAATCGACCTGAAAACAGGAATAGTAACGCGGACGCCATTGTCGACCAGAAACCTAGACTTTGGAGTGATACATCCCTCGTACGTCGGGAAGAAGCACAGGTTCGTTTACGCCGGTGTTGGAGATCCGATGCCCAAGATTTCGGGAGTGGTGAAGCTCGACATATCTCAGGAAGAACGTCGAGACTGCATCGTCGCTTGTAGAATTTTCGGACCTGGTTGCTATGGCGGGGAACCGTTTTTCGTGCCGAGGGAAAGGGAACGTTCCGACGAGACAGAGGCAGAGGAGGACGACGGATATGTCGTCTCATACGTTCATGATGAAAACTCCGGTGAGTCGAGGTTCATTGTCATGGACGCGAAATCGCCGGAGCTGGAAATTATCGCCGCCGTGAAGCTGCCGCGACGCGTCCCTTACGGTTTTCATGGATTGTTCGTGAAAGAAAGCGACCTCAATAAGCTATGA

Protein sequence

MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPSPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL*
Homology
BLAST of CSPI04G06970 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.7e-225
Identity = 389/600 (64.83%), Postives = 475/600 (79.17%), Query Frame = 0

Query: 1   MDSI-SSPFLSGR-NLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPR 60
           MDS+ SS FLS   +L  S     S  P    I S + E+      P  + D  + P   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTL 60

Query: 61  PSPPSPPMPRVSSTRRVQP--SLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV 120
            +  +  +  VSS  +++P  +L    F   +D+IN FI+PP  PSVDP+++L+DNFAPV
Sbjct: 61  HNRTNHTL--VSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPV 120

Query: 121 -DELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
            DELPPT+CE+I+G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G+A L
Sbjct: 121 LDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATL 180

Query: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240
           CSRYVKTYKY +E+  G PV PNVFSGFNG+TAS ARGA+   R+LTGQYNP NGIGLAN
Sbjct: 181 CSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300
           TSLAFF +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL +SMTAHPK D  TGE F
Sbjct: 241 TSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETF 300

Query: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIG--INP 360
           AFRYGP+PPFLTYFRFD  G K  DVPI SM  PSFLHDFAITK++A+F +IQ+G  +N 
Sbjct: 301 AFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNM 360

Query: 361 TQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVV 420
             +++EGGSPVG+D  K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VV
Sbjct: 361 LDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVV 420

Query: 421 IVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHR 480
           ++APNI+S+EH LERMDLVHALVEK++IDL TGIV R P+S RNLDF VI+P+++G+  R
Sbjct: 421 LIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSR 480

Query: 481 FVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEA 540
           +VYA +GDPMPKISGVVKLD+S+ +R DC VA R++G GCYGGEPFFV R+       EA
Sbjct: 481 YVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PGNPEA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           EEDDGYVV+YVHDE +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 EEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of CSPI04G06970 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 5.9e-194
Identity = 338/591 (57.19%), Postives = 433/591 (73.27%), Query Frame = 0

Query: 14  LILSPPISSSLPPISTP------IYSVLTEQNVKKNTPPPDADSPSPPLPRPSPPSPPMP 73
           ++ SP ++++  P  T       +    T + V   T    A S S P  RP+P      
Sbjct: 57  VLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAP------ 116

Query: 74  RVSSTRRVQPSLPARFFNAFDDLINNFINPP-VSPSVDPRYILADNFAPVDELPPTECEV 133
              +      SLP  F NA +++IN FI+PP + P+VDPR +L  NF PVDELPPT C V
Sbjct: 117 ---AAAASAASLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCPV 176

Query: 134 IYGSLPSSL-NGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI-----SDGRAVLCSRYV 193
           + G++P  L  GAYIRNGPNPQ+LPRGP+HLFDGDGMLHSL +     S    VLCSRYV
Sbjct: 177 VRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRYV 236

Query: 194 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 253
           +TYKY +ERDAG PV PNVFSGF+G+ A  ARGAV   R+LTGQ NP  G+GLANTSLA+
Sbjct: 237 QTYKYLVERDAGAPVLPNVFSGFHGV-AGMARGAVVAARVLTGQMNPLEGVGLANTSLAY 296

Query: 254 FGDRLYALGESDLPYPIRLTPN-GDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 313
           F  RLYALGESDLPY +R+ P+ G++ T  R DF G+L + MTAHPK D  TGE FAFRY
Sbjct: 297 FAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRY 356

Query: 314 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 373
           GP+PPF+TYFRFD  G K +DVPI S+ +PSFLHDFAIT++YA+F +IQI + P  M++ 
Sbjct: 357 GPVPPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVG 416

Query: 374 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDE--DDAVVIVAPNI 433
           GGSPVGSDP K+ R+G+IPRYA DES+M+WF+VPG N++H++NAW+E   + +V+VAPN+
Sbjct: 417 GGSPVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNV 476

Query: 434 LSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGV 493
           LS+EHALE M+LVH+ VEK+RI+L+TG+VTRTPL+  N DF VI+P+++G+++R+ Y GV
Sbjct: 477 LSIEHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFGV 536

Query: 494 GDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGY 553
           GDP PKI GV KLD  +    DC VA R FGPGC+ GEPFFV  + E +      EDDGY
Sbjct: 537 GDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGN----GNEDDGY 596

Query: 554 VVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           +V YVHDE +GE+RF+VMDA+SP+LEI+A V+LP RVPYGFHGLFV +++L
Sbjct: 597 LVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAEL 633

BLAST of CSPI04G06970 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 5.7e-165
Identity = 289/523 (55.26%), Postives = 367/523 (70.17%), Query Frame = 0

Query: 78  SLPARFFNAFDDLINNFIN-PPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSL- 137
           SLP    N  D +IN FI+ P   P+VDPR +L+ NFAPVDELPPT C VI GS+PS L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 138 NGAYIRNGPNPQY-LPRGPYHLFDGDGMLHSLRISDGRA------VLCSRYVKTYKYTLE 197
            G YIRNGPNPQ+ LP+  +HLFDGDGMLHSL I    +      VLCSRYV TYKY LE
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 198 RDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYAL 257
           R+ G PV PN F+GF+G+ A  AR  V + R+L GQ N   G GLANTS+  F D LYAL
Sbjct: 175 RETGGPVLPNFFAGFHGV-AGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYAL 234

Query: 258 GESDLPYPIRLTP-NGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPLPPFLT 317
            ESDLPY + + P NG++ TL R DF G L+  MTAHPK D  T E FAFRY    PF+T
Sbjct: 235 CESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFIT 294

Query: 318 YFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSD 377
           YF FD+ G+K +DVPILS+ +PS +HDFAIT++YA+F + Q+ +NP  M++ G S VG D
Sbjct: 295 YFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLD 354

Query: 378 PSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDA--VVIVAPNILSVEHALE 437
            + + R+G++PRYA DES M+WF+VP  N++H  N W+E D   +V+VAPNILS+EH L 
Sbjct: 355 RTMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLG 414

Query: 438 RMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKIS 497
            M+L+ A V+ +RI+L TG V+ T LS  +L+FGVIH  YVG+K+R+ Y GV  P+PKI 
Sbjct: 415 NMELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIK 474

Query: 498 GVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVSYVHDE 557
           G+ KLD       DC V  R FG GC+ GEPFFVP     + +    ED GYVV Y H+E
Sbjct: 475 GIRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVP----DNIDGYGNEDSGYVVCYTHEE 534

Query: 558 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           ++GES F+VMDAKSPEL+I+A V+LP R+PYGFHG+FVK+++L
Sbjct: 535 DTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVKQAEL 572

BLAST of CSPI04G06970 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.6e-122
Identity = 216/369 (58.54%), Postives = 271/369 (73.44%), Query Frame = 0

Query: 224 QYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTP-NGDIETLARHDFDGKLTLSMT 283
           Q +P  GIGLANTSL F   RL+AL E DLPY +RL+P +GDI T+ R + +   T S T
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGRIE-NNVSTKSTT 61

Query: 284 AHPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHS-DVPILSMNRPSFLHDFAITKKY 343
           AHPK D  TGE F+F YGP+ P++TY R+D +G K   DVPI S   PSF+HDFAIT+ Y
Sbjct: 62  AHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHY 121

Query: 344 AVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAI 403
           AVF DIQI + P + I+ G   +G D  K+ R+GL+PRYA  +S+M+WFDVPG N++H +
Sbjct: 122 AVFPDIQIVMKPAE-IVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVV 181

Query: 404 NAWDED--DAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFG 463
           NAW+E+  + VVIVAPN+  +E+A++R DL+H  VE  RI+LK+G V+RT LS  NLDFG
Sbjct: 182 NAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDFG 241

Query: 464 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFV 523
           VIH  Y G+K R+ Y GVGDPMPKI GVVK+D     R +C+VA R FG GC+GGEPFFV
Sbjct: 242 VIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFFV 301

Query: 524 PRERERSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 583
           P   ++S     EEDDGYVVSY+HDE  GES F+VMDA+SPELEI+A V LPRRVPYGFH
Sbjct: 302 PASSKKSG---GEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGFH 361

Query: 584 GLFVKESDL 589
           GLFV E++L
Sbjct: 362 GLFVTEAEL 365

BLAST of CSPI04G06970 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.9e-105
Identity = 229/611 (37.48%), Postives = 339/611 (55.48%), Query Frame = 0

Query: 3   SISSPFLSGRNLILSPPISSSLPP-----ISTPIYSVLTEQ-NVKKNTPPPDADSPSPPL 62
           ++S  +L G +    PP+SSS         S P+ S +T + NV      P    P+   
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTP----PALHF 68

Query: 63  PRPSPPSPPM---PRVSSTRRVQPSLPARFFNAFDDLINNFINP-----PVSPSVDPRYI 122
           P+ S  SP +   P+   +   Q +L  R   A  D    F+       P+  + DP   
Sbjct: 69  PKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQ 128

Query: 123 LADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI 182
           +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++ 
Sbjct: 129 IAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF 188

Query: 183 SDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPA 242
             G A    R+ +T ++  ER  G PVFP      +G T   AR  +   R   G  +PA
Sbjct: 189 EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPA 248

Query: 243 NGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVD 302
           +G G+AN  L +F  RL A+ E DLPY +++TPNGD++T+ R DFDG+L  +M AHPKVD
Sbjct: 249 HGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVD 308

Query: 303 SDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDI 362
            ++GE FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFAIT+ + V  D 
Sbjct: 309 PESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQ 368

Query: 363 QIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWD-- 422
           Q+     +M I GGSPV  D +K++R G++ +YA D S +KW D P     H  NAW+  
Sbjct: 369 QVVFKLPEM-IRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEP 428

Query: 423 EDDAVVIVAPNILSVEHALERMD-LVHALVEKIRIDLKTGIVTRTPLSTR-----NLDFG 482
           E D VV++   +   +      D  + +++ +IR++LKTG  TR P+ +      NL+ G
Sbjct: 429 ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAG 488

Query: 483 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFV 542
           +++ + +G+K +F Y  + +P PK+SG  K+D++  E     V   ++G   YGGEP F+
Sbjct: 489 MVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFL 548

Query: 543 PRERERSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 591
           P E         EED+GY++ +VHDE + +S   +++A S  LE+ A VKLP RVPYGFH
Sbjct: 549 PGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFH 596

BLAST of CSPI04G06970 vs. ExPASy TrEMBL
Match: A0A0A0KV99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056640 PE=3 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 589/591 (99.66%), Postives = 590/591 (99.83%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60
           MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60

Query: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120
           PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP
Sbjct: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120

Query: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180
           PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV
Sbjct: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180

Query: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240
           KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF
Sbjct: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240

Query: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300
           FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG
Sbjct: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300

Query: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360
           PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG
Sbjct: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360

Query: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420
           GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV
Sbjct: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420

Query: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480
           EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP
Sbjct: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480

Query: 481 MPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVS 540
           MPKISGVVKL+ISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDETEAEEDDGYVVS
Sbjct: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540

Query: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of CSPI04G06970 vs. ExPASy TrEMBL
Match: A0A5A7TP93 (Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G003220 PE=3 SV=1)

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 558/592 (94.26%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYAG+GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYAGIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. ExPASy TrEMBL
Match: A0A5D3CY96 (Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002650 PE=3 SV=1)

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 557/592 (94.09%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECE+IYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYAG+GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYAGIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. ExPASy TrEMBL
Match: A0A1S3BSH4 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493037 PE=3 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 556/592 (93.92%), Postives = 569/592 (96.11%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECE+IYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYA +GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYACIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.2e-304
Identity = 513/594 (86.36%), Postives = 548/594 (92.26%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQ---NVKKNTPPPDADSPSPPLP 60
           MD+ISSPFLSG NL+LS PIS S   +ST I SVL E+    VKK    PDAD PSP L 
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  RPSPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVD 120
           + + P P + + SSTRRV+PS+PA  FNAFDDLINNFINPPVSPSVDPRY+LADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCS 180
           ELPPTECEVI GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTS 240
           RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVA GR+LTGQYNPANGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAF 300
           +AFFGDRLYALGESDLPYPIRLTPNGDIETL RHDFDGKL++SMTAHPK+D DTGEAFAF
Sbjct: 241 VAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMI 360
           RYGPLPPFLT+FRFDKNGAK  DVPI SMNRPSFLHDFAITKKYAVFT+IQIG NP QMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 IEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNI 420
           +EG SPVG+DPSKISR+GLIPRYANDESKMKWFDVPG+NL+HAINAWDEDDAV++VAPNI
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNI 420

Query: 421 LSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGV 480
           LSVEH +ERMDLVHALVEKIRIDLKTGI+TR PLSTRNLDFGVI+P YVGKK+RFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGY 540
           GDPMPKISGVVKLD+SQEERRDCIVA R+FGP CYGGEPFFVPRERE SDET AEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           VVSYVHDENSG+SRF+VMDAKSPELEIIA VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of CSPI04G06970 vs. NCBI nr
Match: XP_004147771.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis sativus] >KGN53473.1 hypothetical protein Csa_014438 [Cucumis sativus])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 589/591 (99.66%), Postives = 590/591 (99.83%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60
           MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60

Query: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120
           PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP
Sbjct: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120

Query: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180
           PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV
Sbjct: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180

Query: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240
           KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF
Sbjct: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240

Query: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300
           FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG
Sbjct: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300

Query: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360
           PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG
Sbjct: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360

Query: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420
           GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV
Sbjct: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420

Query: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480
           EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP
Sbjct: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480

Query: 481 MPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVS 540
           MPKISGVVKL+ISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDETEAEEDDGYVVS
Sbjct: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540

Query: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of CSPI04G06970 vs. NCBI nr
Match: KAA0045010.1 (putative carotenoid cleavage dioxygenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 558/592 (94.26%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYAG+GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYAGIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. NCBI nr
Match: TYK16465.1 (putative carotenoid cleavage dioxygenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 557/592 (94.09%), Postives = 570/592 (96.28%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECE+IYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYAG+GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYAGIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. NCBI nr
Match: XP_008451876.1 (PREDICTED: probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 556/592 (93.92%), Postives = 569/592 (96.11%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSP-PLPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECE+IYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYA +GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYACIGD 480

Query: 481 PMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVV 540
           PMPKISGV KLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERE SDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of CSPI04G06970 vs. NCBI nr
Match: XP_038897022.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 536/588 (91.16%), Postives = 559/588 (95.07%), Query Frame = 0

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60
           MD+ISS FLSG NL+LSPPIS S PPIST IYSVLTE+ VKK  P PDADSPSP LPR +
Sbjct: 1   MDAISSSFLSGGNLLLSPPISISRPPISTAIYSVLTEETVKKTAPRPDADSPSPSLPRRA 60

Query: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120
            P P + + SSTRR++PSLPARFFNAFDDLINNFINPPVSPSVDPRY+LADNFAPVDELP
Sbjct: 61  SPPPAIAKGSSTRRIEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVDELP 120

Query: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180
           PTECEVI+GSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRYV
Sbjct: 121 PTECEVIHGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYV 180

Query: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240
           KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVA GR+LTGQ+NPANGIGLANTSLAF
Sbjct: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQFNPANGIGLANTSLAF 240

Query: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300
           FGDRLYALGESDLPYPIRLTPNGDIETL RHDFDGKLT+SMTAHPKVDSDTGEAFAFRYG
Sbjct: 241 FGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLTMSMTAHPKVDSDTGEAFAFRYG 300

Query: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360
           PLPPFLT+FRFDKNGAK SDVPI SMNRPSFLHDFAITKKYAVFT+IQIG NP QM IEG
Sbjct: 301 PLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQM-IEG 360

Query: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420
            SPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVV++APNILSV
Sbjct: 361 RSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVLIAPNILSV 420

Query: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480
           EH LERMDLVHALVEK+RIDLKTGIVTR PLSTRNLDFGVIHPSYVGKK+RFVYAGVGDP
Sbjct: 421 EHTLERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVIHPSYVGKKNRFVYAGVGDP 480

Query: 481 MPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVS 540
           MPKISGVVKLD+SQEERRDCIVACRIFGPGCYGGEPFFVPRERE S+ET AEEDDGYVVS
Sbjct: 481 MPKISGVVKLDVSQEERRDCIVACRIFGPGCYGGEPFFVPRERESSEETPAEEDDGYVVS 540

Query: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL
Sbjct: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 587

BLAST of CSPI04G06970 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 783.5 bits (2022), Expect = 1.2e-226
Identity = 389/600 (64.83%), Postives = 475/600 (79.17%), Query Frame = 0

Query: 1   MDSI-SSPFLSGR-NLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPR 60
           MDS+ SS FLS   +L  S     S  P    I S + E+      P  + D  + P   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTL 60

Query: 61  PSPPSPPMPRVSSTRRVQP--SLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV 120
            +  +  +  VSS  +++P  +L    F   +D+IN FI+PP  PSVDP+++L+DNFAPV
Sbjct: 61  HNRTNHTL--VSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPV 120

Query: 121 -DELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
            DELPPT+CE+I+G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G+A L
Sbjct: 121 LDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATL 180

Query: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240
           CSRYVKTYKY +E+  G PV PNVFSGFNG+TAS ARGA+   R+LTGQYNP NGIGLAN
Sbjct: 181 CSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300
           TSLAFF +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL +SMTAHPK D  TGE F
Sbjct: 241 TSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETF 300

Query: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIG--INP 360
           AFRYGP+PPFLTYFRFD  G K  DVPI SM  PSFLHDFAITK++A+F +IQ+G  +N 
Sbjct: 301 AFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNM 360

Query: 361 TQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVV 420
             +++EGGSPVG+D  K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VV
Sbjct: 361 LDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVV 420

Query: 421 IVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHR 480
           ++APNI+S+EH LERMDLVHALVEK++IDL TGIV R P+S RNLDF VI+P+++G+  R
Sbjct: 421 LIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSR 480

Query: 481 FVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEA 540
           +VYA +GDPMPKISGVVKLD+S+ +R DC VA R++G GCYGGEPFFV R+       EA
Sbjct: 481 YVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PGNPEA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           EEDDGYVV+YVHDE +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 EEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of CSPI04G06970 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 383.6 bits (984), Expect = 2.8e-106
Identity = 229/611 (37.48%), Postives = 339/611 (55.48%), Query Frame = 0

Query: 3   SISSPFLSGRNLILSPPISSSLPP-----ISTPIYSVLTEQ-NVKKNTPPPDADSPSPPL 62
           ++S  +L G +    PP+SSS         S P+ S +T + NV      P    P+   
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTP----PALHF 68

Query: 63  PRPSPPSPPM---PRVSSTRRVQPSLPARFFNAFDDLINNFINP-----PVSPSVDPRYI 122
           P+ S  SP +   P+   +   Q +L  R   A  D    F+       P+  + DP   
Sbjct: 69  PKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQ 128

Query: 123 LADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI 182
           +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++ 
Sbjct: 129 IAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF 188

Query: 183 SDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPA 242
             G A    R+ +T ++  ER  G PVFP      +G T   AR  +   R   G  +PA
Sbjct: 189 EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPA 248

Query: 243 NGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVD 302
           +G G+AN  L +F  RL A+ E DLPY +++TPNGD++T+ R DFDG+L  +M AHPKVD
Sbjct: 249 HGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVD 308

Query: 303 SDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDI 362
            ++GE FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFAIT+ + V  D 
Sbjct: 309 PESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQ 368

Query: 363 QIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWD-- 422
           Q+     +M I GGSPV  D +K++R G++ +YA D S +KW D P     H  NAW+  
Sbjct: 369 QVVFKLPEM-IRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEP 428

Query: 423 EDDAVVIVAPNILSVEHALERMD-LVHALVEKIRIDLKTGIVTRTPLSTR-----NLDFG 482
           E D VV++   +   +      D  + +++ +IR++LKTG  TR P+ +      NL+ G
Sbjct: 429 ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAG 488

Query: 483 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIFGPGCYGGEPFFV 542
           +++ + +G+K +F Y  + +P PK+SG  K+D++  E     V   ++G   YGGEP F+
Sbjct: 489 MVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFL 548

Query: 543 PRERERSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 591
           P E         EED+GY++ +VHDE + +S   +++A S  LE+ A VKLP RVPYGFH
Sbjct: 549 PGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFH 596

BLAST of CSPI04G06970 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 382.9 bits (982), Expect = 4.7e-106
Identity = 213/538 (39.59%), Postives = 306/538 (56.88%), Query Frame = 0

Query: 70  SSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV-DELPPTECEVIY 129
           SS   V P     F +   DL+   +   +  +  P + L+ NFAP+ DE PP +   ++
Sbjct: 9   SSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVH 68

Query: 130 GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLE 189
           G LP  LNG ++R GPNP++     YH FDGDGM+H +RI DG+A   SRYVKT +   E
Sbjct: 69  GFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQE 128

Query: 190 RDAGHPVFPNV--FSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLY 249
              G   F  +    GF GL     +      +IL   Y    G G ANT+L +   +L 
Sbjct: 129 EFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTY----GNGTANTALVYHHGKLL 188

Query: 250 ALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPLPPFL 309
           AL E+D PY I++  +GD++TL   D+D +LT S TAHPKVD  TGE F F Y   PP+L
Sbjct: 189 ALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYL 248

Query: 310 TYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGS 369
           TY    K+G  H  VPI +++ P  +HDFAIT+ YA+F D+ +   P +M+ E       
Sbjct: 249 TYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSF 308

Query: 370 DPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVA-----PNILSVE 429
           DP+K +R G++PRYA DE  ++WF++P   + H  NAW+E+D VV++      P++  V 
Sbjct: 309 DPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVS 368

Query: 430 HAL-ERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 489
             + E+++     + ++R ++KTG  ++  LS   +DF  I+  Y GKK R+VY  + D 
Sbjct: 369 GKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDS 428

Query: 490 MPKISGVVKLDISQEE---RRDCIVACRI-----FGPGCYGGEPFFVPRERERSDETEAE 549
           + K++G++K D+  E    +R   V   I      G G YG E  +VPRE        AE
Sbjct: 429 IAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE-------TAE 488

Query: 550 EDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNK 591
           EDDGY++ +VHDEN+G+S   V+DAK+   E +A V+LP RVPYGFH LFV E  L +
Sbjct: 489 EDDGYLIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQLQE 534

BLAST of CSPI04G06970 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 372.9 bits (956), Expect = 4.9e-103
Identity = 220/582 (37.80%), Postives = 324/582 (55.67%), Query Frame = 0

Query: 19  PISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPS----PPLPRPSPPSPPMPRVSSTRR 78
           P     P  S+ +    T+   +K +    +D+P+    P  P P+P  P     S    
Sbjct: 20  PSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKD-TSRWNP 79

Query: 79  VQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSS 138
           +Q +  A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +P  
Sbjct: 80  LQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDC 139

Query: 139 LNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHP 198
           ++G Y+RNG NP + P   +HLFDGDGM+H+++I++G A    R+ +T +   E+  G P
Sbjct: 140 IDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSP 199

Query: 199 VFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLP 258
           +FP      +G  +  AR  +   R L G  N  NG G+AN  L +F DRL A+ E DLP
Sbjct: 200 IFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLP 259

Query: 259 YPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDK 318
           Y +R+T NGD+ET+ R DFDG+L+ +M AHPK+D  T E FA  Y  +  P+L YF+F  
Sbjct: 260 YQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSP 319

Query: 319 NGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISR 378
            G K  DV I  +  P+ +HDFAIT+ + V  D Q+    + M + G SPV  D  KISR
Sbjct: 320 EGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKISR 379

Query: 379 VGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVIVAPNILSVEHALERMD-LV 438
            G++PR A D S+M W + P     H  NAW+  E D VV++   +   +      D  +
Sbjct: 380 FGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQL 439

Query: 439 HALVEKIRIDLKTGIVTRTPL----STRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKISG 498
           ++++ +IR++LKTG  TR  +       NL+ G+++ + +G+K R+ Y  + +P PK+SG
Sbjct: 440 NSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSG 499

Query: 499 VVKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERERSDETEAEEDDGYVVSYVHDEN 558
             K+D+S  E     V    +G   YGGEPFF+PR  E        EDDGY++S+VHDE 
Sbjct: 500 FAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDEE 559

Query: 559 SGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           S ES   +++A + ELE  A VKLP RVPYGFHG FV  +D+
Sbjct: 560 SWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of CSPI04G06970 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 365.9 bits (938), Expect = 6.0e-101
Identity = 196/503 (38.97%), Postives = 291/503 (57.85%), Query Frame = 0

Query: 98  PVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 157
           P   + DP   +A NF PV E P      + G++P  + G Y+RNG NP + P   +HLF
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 158 DGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAV 217
           DGDGM+H++R  +G      R+ +T +   ER+ G PVFP      +G     A+  +  
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHG-HLGIAKLMLFN 291

Query: 218 GRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKL 277
            R L G  +P  G+G+AN  L +F   L A+ E DLPY +++T  GD+ET  R+DFDG+L
Sbjct: 292 TRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQL 351

Query: 278 TLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFA 337
             +M AHPK+D +T E FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFA
Sbjct: 352 KSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFA 411

Query: 338 ITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLN 397
           IT+ + V  D Q+     +M I GGSPV  D  K SR G++ + A D S ++W +VP   
Sbjct: 412 ITENFVVIPDQQVVFRLPEM-IRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCF 471

Query: 398 LIHAINAWDE---DDAVVI----VAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRT 457
             H  N+W+E   D+ VVI      P+ +  EH     + + +++ +IR++LKTG  TR 
Sbjct: 472 CFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHD----ETLQSVLSEIRLNLKTGESTRR 531

Query: 458 PLSTR--NLDFGVIHPSYVGKKHRFVYAGVGDPMPKISGVVKLDISQEERRDCIVACRIF 517
           P+ +   NL+ G+++ + +G+K R+ Y  + +P PK+SG  K+D+S  E R       I+
Sbjct: 532 PVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRK-----YIY 591

Query: 518 GPGCYGGEPFFVPRERERSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAA 577
           G G YGGEP F+P         + EED GY++ +VHDE   +S   +++A + +LE  A 
Sbjct: 592 GEGKYGGEPLFLP-------SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--AT 651

Query: 578 VKLPRRVPYGFHGLFVKESDLNK 591
           V LP RVPYGFHG F+ + DL+K
Sbjct: 652 VTLPSRVPYGFHGTFISKEDLSK 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496751.7e-22564.83Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thalian... [more]
Q6YVJ05.9e-19457.199-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q5MBR65.7e-16555.269-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q84K961.6e-12258.54Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=8... [more]
Q9LRR73.9e-10537.489-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A0A0KV990.0e+0099.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056640 PE=3 SV=1[more]
A0A5A7TP930.0e+0094.26Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5D3CY960.0e+0094.09Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BSH40.0e+0093.92probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucumis melo OX=365... [more]
A0A346DA054.2e-30486.36Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147771.10.0e+0099.66probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis sativus] >KGN... [more]
KAA0045010.10.0e+0094.26putative carotenoid cleavage dioxygenase 4 [Cucumis melo var. makuwa][more]
TYK16465.10.0e+0094.09putative carotenoid cleavage dioxygenase 4 [Cucumis melo var. makuwa][more]
XP_008451876.10.0e+0093.92PREDICTED: probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis me... [more]
XP_038897022.10.0e+0091.16probable carotenoid cleavage dioxygenase 4, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G19170.11.2e-22664.83nine-cis-epoxycarotenoid dioxygenase 4 [more]
AT3G14440.12.8e-10637.48nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT3G63520.14.7e-10639.59carotenoid cleavage dioxygenase 1 [more]
AT1G30100.14.9e-10337.80nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT1G78390.16.0e-10138.97nine-cis-epoxycarotenoid dioxygenase 9 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 114..583
e-value: 1.5E-107
score: 360.7
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 63..590
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..74
NoneNo IPR availablePANTHERPTHR10543:SF46CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATEDcoord: 63..590

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G06970.1CSPI04G06970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen