CSPI04G06730 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G06730
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	aspartic proteinase CDR1-like
Location	Chr4: 4712365 .. 4713657 (-)
RNA-Seq Expression	CSPI04G06730
Synteny	CSPI04G06730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTACCATTTCCCTCTTCTTCCATCTAATTCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGTGATTCCCTTCTTTCCCCTCTTGAATTTTCATCTCTATCTCATTATGACCGCCTCGCCAATGCCTTTCGTCGCTCATTGTCACGCTCTGCTGCTCTCCTCAATCGCGCTGCCACTAGTGGTGCCGTTGGCCTTCAATCCTCAATTGGTCCAGGAAGTGGCGAGTATCTAATGTCCGTCTCTATTGGAACCCCACCAGTTGATTACTTAGGCATTGCTGACACGGGCAGTGATCTGACATGGGCTCAGTGCTTGCCATGTCTGAAATGCTACCAACAATTACGTCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCAATACCCAGACCTGTCACGCTGTTGATGATGGCCATTGTGGGGTTCAGGGGGTTTGCGATTACAGTTACACATACGGAGATCGAACTTACTCAAAGGGGGATTTGGGATTTGAAAAGATAACCATTGGGTCATCTTCTGTGAAATCAGTCATTGGATGCGGCCATGCGAGTAGTGGCGGATTTGGGTTTGCTTCAGGTGTCATTGGACTTGGTGGTGGTCACCTCTCGTTGGTCTCACAAATGAGCCAAACCTCCGGTATCAGCCGTCGATTCTCTTATTGCTTACCAACGTTGCTCAGTCACGCAAATGGCAAAATAAACTTTGGCGAAAACGCTGTGGTTTCTGGCCCTGGAGTCGTTTCAACGCCACTAATCTCCAAAAACACCGTCACTTACTATTACATCACTTTGGAAGCCATTTCCATCGGCAACGAACGTCACATGGCCTTTGCCAAACAAGGCAATGTGATTATCGATAGTGGGACGACATTAACGATTCTTCCAAAGGAATTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGAAGGATCCCCATGGTTCTTTGGATCTATGCTTTGATGATGGCATCAACGTCGCTGCCTCCTTGGGTATTCCAGTTATCACTGCGCATTTTTTCGGTGGCGCTAACGTGAATTTGTTGCCAATTAATACGTTTCGGAAGGTGGCAGATAATGTGAATTGCTTGACATTAAAAGCTGCATCGCCGACAACCGAATTTGGGATAATTGGAAATTTGGCGCAAGCCAATTTCTTGATCGGATATGATTTGGAGGCCAAGAGATTGTCATTCAAGCCAACCGTTTGTGCCTAG

mRNA sequence

ATGGCTGCTACCATTTCCCTCTTCTTCCATCTAATTCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGTGATTCCCTTCTTTCCCCTCTTGAATTTTCATCTCTATCTCATTATGACCGCCTCGCCAATGCCTTTCGTCGCTCATTGTCACGCTCTGCTGCTCTCCTCAATCGCGCTGCCACTAGTGGTGCCGTTGGCCTTCAATCCTCAATTGGTCCAGGAAGTGGCGAGTATCTAATGTCCGTCTCTATTGGAACCCCACCAGTTGATTACTTAGGCATTGCTGACACGGGCAGTGATCTGACATGGGCTCAGTGCTTGCCATGTCTGAAATGCTACCAACAATTACGTCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCAATACCCAGACCTGTCACGCTGTTGATGATGGCCATTGTGGGGTTCAGGGGGTTTGCGATTACAGTTACACATACGGAGATCGAACTTACTCAAAGGGGGATTTGGGATTTGAAAAGATAACCATTGGGTCATCTTCTGTGAAATCAGTCATTGGATGCGGCCATGCGAGTAGTGGCGGATTTGGGTTTGCTTCAGGTGTCATTGGACTTGGTGGTGGTCACCTCTCGTTGGTCTCACAAATGAGCCAAACCTCCGGTATCAGCCGTCGATTCTCTTATTGCTTACCAACGTTGCTCAGTCACGCAAATGGCAAAATAAACTTTGGCGAAAACGCTGTGGTTTCTGGCCCTGGAGTCGTTTCAACGCCACTAATCTCCAAAAACACCGTCACTTACTATTACATCACTTTGGAAGCCATTTCCATCGGCAACGAACGTCACATGGCCTTTGCCAAACAAGGCAATGTGATTATCGATAGTGGGACGACATTAACGATTCTTCCAAAGGAATTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGAAGGATCCCCATGGTTCTTTGGATCTATGCTTTGATGATGGCATCAACGTCGCTGCCTCCTTGGGTATTCCAGTTATCACTGCGCATTTTTTCGGTGGCGCTAACGTGAATTTGTTGCCAATTAATACGTTTCGGAAGGTGGCAGATAATGTGAATTGCTTGACATTAAAAGCTGCATCGCCGACAACCGAATTTGGGATAATTGGAAATTTGGCGCAAGCCAATTTCTTGATCGGATATGATTTGGAGGCCAAGAGATTGTCATTCAAGCCAACCGTTTGTGCCTAG

Coding sequence (CDS)

ATGGCTGCTACCATTTCCCTCTTCTTCCATCTAATTCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGTGATTCCCTTCTTTCCCCTCTTGAATTTTCATCTCTATCTCATTATGACCGCCTCGCCAATGCCTTTCGTCGCTCATTGTCACGCTCTGCTGCTCTCCTCAATCGCGCTGCCACTAGTGGTGCCGTTGGCCTTCAATCCTCAATTGGTCCAGGAAGTGGCGAGTATCTAATGTCCGTCTCTATTGGAACCCCACCAGTTGATTACTTAGGCATTGCTGACACGGGCAGTGATCTGACATGGGCTCAGTGCTTGCCATGTCTGAAATGCTACCAACAATTACGTCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCAATACCCAGACCTGTCACGCTGTTGATGATGGCCATTGTGGGGTTCAGGGGGTTTGCGATTACAGTTACACATACGGAGATCGAACTTACTCAAAGGGGGATTTGGGATTTGAAAAGATAACCATTGGGTCATCTTCTGTGAAATCAGTCATTGGATGCGGCCATGCGAGTAGTGGCGGATTTGGGTTTGCTTCAGGTGTCATTGGACTTGGTGGTGGTCACCTCTCGTTGGTCTCACAAATGAGCCAAACCTCCGGTATCAGCCGTCGATTCTCTTATTGCTTACCAACGTTGCTCAGTCACGCAAATGGCAAAATAAACTTTGGCGAAAACGCTGTGGTTTCTGGCCCTGGAGTCGTTTCAACGCCACTAATCTCCAAAAACACCGTCACTTACTATTACATCACTTTGGAAGCCATTTCCATCGGCAACGAACGTCACATGGCCTTTGCCAAACAAGGCAATGTGATTATCGATAGTGGGACGACATTAACGATTCTTCCAAAGGAATTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGAAGGATCCCCATGGTTCTTTGGATCTATGCTTTGATGATGGCATCAACGTCGCTGCCTCCTTGGGTATTCCAGTTATCACTGCGCATTTTTTCGGTGGCGCTAACGTGAATTTGTTGCCAATTAATACGTTTCGGAAGGTGGCAGATAATGTGAATTGCTTGACATTAAAAGCTGCATCGCCGACAACCGAATTTGGGATAATTGGAAATTTGGCGCAAGCCAATTTCTTGATCGGATATGATTTGGAGGCCAAGAGATTGTCATTCAAGCCAACCGTTTGTGCCTAG

Protein sequence

MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSYCLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQGNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFKPTVCA*

Homology

[Close]

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.5e-87
Identity = 195/451 (43.24%), Postives = 272/451 (60.31%), Query Frame = 0


Query: 11  LILFLISFSQTTIING-DNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSRSAAL 70
           L+ F + FS T   +G    F+  L HRDS LSP+    ++  DRL  AF RS+SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 71  LNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQ 130
            ++ + +    LQS +    GE+ MS++IGTPP+    IADTGSDLTW QC PC +CY++
Sbjct: 66  NHQLSQT---DLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKE 125

Query: 131 LRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGV---QGVCDYSYTYGDRTYSKGDLGFEK 190
             PIF+  KS+++   PC+++ C A+     G      +C Y Y+YGD+++SKGD+  E 
Sbjct: 126 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 185

Query: 191 ITIGSSSVK------SVIGCGHASSGGFG-FASGVIGLGGGHLSLVSQMSQTSGISRRFS 250
           ++I S+S        +V GCG+ + G F    SG+IGLGGGHLSL+SQ+   S IS++FS
Sbjct: 186 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFS 245

Query: 251 YCLPTLLSHANGK--INFGENAVVSG----PGVVSTPLISKNTVTYYYITLEAISIG--- 310
           YCL    +  NG   IN G N++ S      GVVSTPL+ K  +TYYY+TLEAIS+G   
Sbjct: 246 YCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKK 305

Query: 311 ----------NERHMAFAKQGNVIIDSGTTLTILPKELYDGVVSSLLK-VVKAKRVKDPH 370
                     N+  +     GN+IIDSGTTLT+L    +D   S++ + V  AKRV DP 
Sbjct: 306 IPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQ 365

Query: 371 GSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTE 430
           G L  CF  G   +A +G+P IT H F GA+V L PIN F K+++++ CL++    PTTE
Sbjct: 366 GLLSHCFKSG---SAEIGLPEITVH-FTGADVRLSPINAFVKLSEDMVCLSM---VPTTE 425

[Close]

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.4e-83
Identity = 187/446 (41.93%), Postives = 267/446 (59.87%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDN----GFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRR 65
           SLF  ++L L   S   + N +     GFT  L HRDS  SP      +   RL NA  R
Sbjct: 3   SLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHR 62

Query: 66  SLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCL 125
           S++R      +  T      Q  +   SGEYLM+VSIGTPP   + IADTGSDL W QC 
Sbjct: 63  SVNRVFHFTEKDNTPQP---QIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 122

Query: 126 PCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDD-GHCGV-QGVCDYSYTYGDRTYSK 185
           PC  CY Q+ P+F+P  S+++  V C++  C A+++   C      C YS +YGD +Y+K
Sbjct: 123 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 182

Query: 186 GDLGFEKITIGSSSVKS------VIGCGHASSGGFG-FASGVIGLGGGHLSLVSQMSQTS 245
           G++  + +T+GSS  +       +IGCGH ++G F    SG++GLGGG +SL+ Q+  + 
Sbjct: 183 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDS- 242

Query: 246 GISRRFSYCLPTLLSHAN--GKINFGENAVVSGPGVVSTPLISK-NTVTYYYITLEAISI 305
            I  +FSYCL  L S  +   KINFG NA+VSG GVVSTPLI+K +  T+YY+TL++IS+
Sbjct: 243 -IDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISV 302

Query: 306 GNERHM-----AFAKQGNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDL 365
           G+++       + + +GN+IIDSGTTLT+LP E Y  +  ++   + A++ +DP   L L
Sbjct: 303 GSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 362

Query: 366 CFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIG 425
           C+    +    L +PVIT H F GA+V L   N F +V++++ C   +  SP+  F I G
Sbjct: 363 CY----SATGDLKVPVITMH-FDGADVKLDSSNAFVQVSEDLVCFAFR-GSPS--FSIYG 422

Query: 426 NLAQANFLIGYDLEAKRLSFKPTVCA 431
           N+AQ NFL+GYD  +K +SFKPT CA
Sbjct: 423 NVAQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 7.6e-58
Identity = 151/413 (36.56%), Postives = 211/413 (51.09%), Query Frame = 0


Query: 29  GFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSRSAALLNRAATSGAVGLQSSIGPG 88
           GF   L H DS  +  +F  L   +R      R L R  A+LN     G  G+++S+  G
Sbjct: 40  GFQIMLEHVDSGKNLTKFQLL---ERAIERGSRRLQRLEAMLN-----GPSGVETSVYAG 99

Query: 89  SGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQLRPIFNPLKSTSFSHVPCN 148
            GEYLM++SIGTP   +  I DTGSDL W QC PC +C+ Q  PIFNP  S+SFS +PC+
Sbjct: 100 DGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCS 159

Query: 149 TQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFEKITIGSSSVKSV-IGCGHASSG- 208
           +Q C A+    C     C Y+Y YGD + ++G +G E +T GS S+ ++  GCG  + G 
Sbjct: 160 SQLCQALSSPTCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGF 219

Query: 209 GFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSYCLPTLLSHANGKINFGENAVVSGPGV 268
           G G  +G++G+G G LSL SQ+  T     +FSYC+  + S     +  G  A     G 
Sbjct: 220 GQGNGAGLVGMGRGPLSLPSQLDVT-----KFSYCMTPIGSSTPSNLLLGSLANSVTAGS 279

Query: 269 VSTPLISKNTV-TYYYITLEAISIGNER----HMAFAKQ-----GNVIIDSGTTLTILPK 328
            +T LI  + + T+YYITL  +S+G+ R      AFA       G +IIDSGTTLT    
Sbjct: 280 PNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVN 339

Query: 329 ELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPI 388
             Y  V    +  +    V       DLCF    +  ++L IP    HF GG ++ L   
Sbjct: 340 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSD-PSNLQIPTFVMHFDGG-DLELPSE 399

Query: 389 NTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFKPTVC 430
           N F   ++ + CL +   S +    I GN+ Q N L+ YD     +SF    C
Sbjct: 400 NYFISPSNGLICLAM--GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.3e-54
Identity = 142/393 (36.13%), Postives = 207/393 (52.67%), Query Frame = 0


Query: 48  SLSHYDRLANAFRRSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLG 107
           +L+ Y+ +  A +R   R  ++   A    + G+++ +  G GEYLM+V+IGTP   +  
Sbjct: 54  NLTKYELIKRAIKRGERRMRSI--NAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSA 113

Query: 108 IADTGSDLTWAQCLPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCD 167
           I DTGSDL W QC PC +C+ Q  PIFNP  S+SFS +PC +Q C  +    C     C 
Sbjct: 114 IMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCN-NNECQ 173

Query: 168 YSYTYGDRTYSKGDLGFEKITIGSSSVKSV-IGCGHASSG-GFGFASGVIGLGGGHLSLV 227
           Y+Y YGD + ++G +  E  T  +SSV ++  GCG  + G G G  +G+IG+G G LSL 
Sbjct: 174 YTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLP 233

Query: 228 SQMSQTSGISRRFSYCLPTLLSHANGKINFGENAVVSGPGVVSTPLI-SKNTVTYYYITL 287
           SQ+    G+  +FSYC+ +  S +   +  G  A     G  ST LI S    TYYYITL
Sbjct: 234 SQL----GVG-QFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITL 293

Query: 288 EAISIGNER----HMAFAKQ----GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVK 347
           + I++G +        F  Q    G +IIDSGTTLT LP++ Y+ V  +    +    V 
Sbjct: 294 QGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVD 353

Query: 348 DPHGSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASP 407
           +    L  CF    +  +++ +P I+  F GG  +NL   N     A+ V CL +  +S 
Sbjct: 354 ESSSGLSTCFQQPSD-GSTVQVPEISMQFDGGV-LNLGEQNILISPAEGVICLAM-GSSS 413

Query: 408 TTEFGIIGNLAQANFLIGYDLEAKRLSFKPTVC 430
                I GN+ Q    + YDL+   +SF PT C
Sbjct: 414 QLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.0e-50
Identity = 138/430 (32.09%), Postives = 210/430 (48.84%), Query Frame = 0


Query: 30  FTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSRSAALLNRAATSGAV---------- 89
           +T  L HRD   S    +  +H+ RL    RR   R +A+L R   SG V          
Sbjct: 59  YTLRLLHRDRFPS---VTYRNHHHRLHARMRRDTDRVSAILRR--ISGKVIPSSDSRYEV 118

Query: 90  -----GLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQLRPIF 149
                 + S +  GSGEY + + +G+PP D   + D+GSD+ W QC PC  CY+Q  P+F
Sbjct: 119 NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVF 178

Query: 150 NPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFEKITIGSSSV 209
           +P KS S++ V C +  C  +++  C   G C Y   YGD +Y+KG L  E +T   + V
Sbjct: 179 DPAKSGSYTGVSCGSSVCDRIENSGCH-SGGCRYEVMYGDGSYTKGTLALETLTFAKTVV 238

Query: 210 KSV-IGCGHASSGGFGFASGVIGLGGGHLSLVSQMS-QTSGISRRFSYCLPTLLSHANGK 269
           ++V +GCGH + G F  A+G++G+GGG +S V Q+S QT G    F YCL +  + + G 
Sbjct: 239 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGG---AFGYCLVSRGTDSTGS 298

Query: 270 INFGENAVVSGPGVVSTPLI-SKNTVTYYYITLEAISIGNER--------HMAFAKQGNV 329
           + FG  A+  G   V  PL+ +    ++YY+ L+ + +G  R         +     G V
Sbjct: 299 LVFGREALPVGASWV--PLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 358

Query: 330 IIDSGTTLTILPKELY----DGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIP 389
           ++D+GT +T LP   Y    DG  S    + +A  V       D C+D  ++   S+ +P
Sbjct: 359 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGV----SIFDTCYD--LSGFVSVRVP 418

Query: 390 VITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEA 430
            ++ +F  G  V  LP   F    D+        A+  T   IIGN+ Q    + +D   
Sbjct: 419 TVSFYFTEGP-VLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGAN 470

[Close]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL
Match: A0A0A0KV20 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G055400 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 426/430 (99.07%), Postives = 427/430 (99.30%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           MAATISLFFHLILFLISFSQTTIING+NGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR
Sbjct: 1   MAATISLFFHLILFLISFSQTTIINGNNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG
Sbjct: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ
Sbjct: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGIN AASLGIPV
Sbjct: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINAAASLGIPV 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           ITAHF GGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITAHFSGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVCA 431
           RLSFKPTVCA
Sbjct: 421 RLSFKPTVCA 430

[Close]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL
Match: A0A5A7TSV3 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002730 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 6.8e-219
Identity = 385/429 (89.74%), Postives = 404/429 (94.17%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           M ATIS+FF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MVATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNRAATSGAVGLQS I PGSGEYLMSVSIGTPPVDY+G+ADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPC+KC++Q RPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLG EKITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLPTLLSHANGKINFG+NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           IT HF GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVC 430
           RLSFKPTVC
Sbjct: 421 RLSFKPTVC 429

[Close]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL
Match: A0A5D3CX41 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003090 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.0e-218
Identity = 384/424 (90.57%), Postives = 402/424 (94.81%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSR 65
           S+F  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLK 125
           SAALLNRAATSGAVGLQS I PGSGEYLMSVSIGTPPVDY+G+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFE 185
           C++Q RPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KGDLG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSYCLPTL 245
           KITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQGNVII 305
           LSHANGKINFG+NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPVITAHF 365
           DSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+ITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 FGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFK 425
            GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAKRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PTVC 430
           PTVC
Sbjct: 424 PTVC 427

[Close]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL
Match: A0A1S3BUB0 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.0e-218
Identity = 384/424 (90.57%), Postives = 402/424 (94.81%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSR 65
           S+F  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLK 125
           SAALLNRAATSGAVGLQS I PGSGEYLMSVSIGTPPVDY+G+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFE 185
           C++Q RPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KGDLG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSYCLPTL 245
           KITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQGNVII 305
           LSHANGKINFG+NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPVITAHF 365
           DSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+ITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 FGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFK 425
            GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAKRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PTVC 430
           PTVC
Sbjct: 424 PTVC 427

[Close]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL
Match: A0A5D3CYR2 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003080 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 8.3e-217
Identity = 383/429 (89.28%), Postives = 400/429 (93.24%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           MAATIS+FF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MAATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNR ATSGAVGLQS I PGSGEYLM VSIGTPPVDY+G+ DTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPC KC+ QLRPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLGFEKITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLP LL HANGKINF +NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQ
Sbjct: 241 CLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           ITAHF GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVC 430
           RLSFKPTVC
Sbjct: 421 RLSFKPTVC 429

[Close]

BLAST of CSPI04G06730 vs. NCBI nr
Match: XP_004149005.3 (probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649217.1 hypothetical protein Csa_015005 [Cucumis sativus])

HSP 1 Score: 845.5 bits (2183), Expect = 2.0e-241
Identity = 427/430 (99.30%), Postives = 427/430 (99.30%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR
Sbjct: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG
Sbjct: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ
Sbjct: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGIN AASLGIPV
Sbjct: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINAAASLGIPV 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           ITAHF GGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITAHFSGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVCA 431
           RLSFKPTVCA
Sbjct: 421 RLSFKPTVCA 430

[Close]

BLAST of CSPI04G06730 vs. NCBI nr
Match: KAA0044967.1 (putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 769.6 bits (1986), Expect = 1.4e-218
Identity = 385/429 (89.74%), Postives = 404/429 (94.17%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           M ATIS+FF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MVATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNRAATSGAVGLQS I PGSGEYLMSVSIGTPPVDY+G+ADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPC+KC++Q RPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLG EKITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLPTLLSHANGKINFG+NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           IT HF GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVC 430
           RLSFKPTVC
Sbjct: 421 RLSFKPTVC 429

[Close]

BLAST of CSPI04G06730 vs. NCBI nr
Match: XP_008452153.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 768.1 bits (1982), Expect = 4.1e-218
Identity = 384/424 (90.57%), Postives = 402/424 (94.81%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSR 65
           S+F  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLK 125
           SAALLNRAATSGAVGLQS I PGSGEYLMSVSIGTPPVDY+G+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFE 185
           C++Q RPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KGDLG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSYCLPTL 245
           KITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQGNVII 305
           LSHANGKINFG+NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPVITAHF 365
           DSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+ITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 FGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFK 425
            GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAKRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PTVC 430
           PTVC
Sbjct: 424 PTVC 427

[Close]

BLAST of CSPI04G06730 vs. NCBI nr
Match: XP_008452152.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 762.7 bits (1968), Expect = 1.7e-216
Identity = 383/429 (89.28%), Postives = 400/429 (93.24%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           MAATIS+FF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MAATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSAALLNR ATSGAVGLQS I PGSGEYLM VSIGTPPVDY+G+ DTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPC KC+ QLRPIFNPLKSTSFSHVPCN+Q C A+DD HCGVQGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLGFEKITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLP LL HANGKINF +NAVVSGPGVVSTPLISK+ VTYYYITLEAISIGNERHMA AKQ
Sbjct: 241 CLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTLT+LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVAAS GIP+
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           ITAHF GGANVNLLP+NTF+KVA+NVNCLTL AASPT EFGIIGNLAQANFLIGYDLEAK
Sbjct: 361 ITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPTVC 430
           RLSFKPTVC
Sbjct: 421 RLSFKPTVC 429

[Close]

BLAST of CSPI04G06730 vs. NCBI nr
Match: XP_031739975.1 (probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetical protein Csa_014406 [Cucumis sativus])

HSP 1 Score: 756.9 bits (1953), Expect = 9.5e-215
Identity = 378/429 (88.11%), Postives = 398/429 (92.77%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           M ATIS+FFHLIL LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL NAFR
Sbjct: 1   MVATISIFFHLILLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RSLSRSA LLNRAAT+GA+ LQ+ + PGSGEYLMSVSIGTPPVDY+G+ADTGSDL WAQC
Sbjct: 61  RSLSRSATLLNRAATNGALDLQAPLTPGSGEYLMSVSIGTPPVDYIGMADTGSDLMWAQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180
           LPCLKCY+Q RPIF+PLKSTSFSHVPCN+Q C A+DD HCG QGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCLKCYKQSRPIFDPLKSTSFSHVPCNSQNCKAIDDSHCGAQGVCDYSYTYGDQTYTKG 180

Query: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGHLSLVSQMSQTSGISRRFSY 240
           DLGFEKITIGSSSVKSVIGCGH S GGFGFASGVIGLGGG LSLVSQMSQTSGISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300
           CLPTLLSHANGKINFG+NAVVSGPGVVSTPLISKN VTYYY+TLEAISIGNERHMA AKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKNPVTYYYVTLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINVAASLGIPV 360
           GNVIIDSGTTL+ LPKELYDGVVSSLLKVVKAKRVKDP    DLCFDDGINVA S GIP+
Sbjct: 301 GNVIIDSGTTLSFLPKELYDGVVSSLLKVVKAKRVKDPGNFWDLCFDDGINVATSSGIPI 360

Query: 361 ITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420
           ITA F GGANVNLLP+NTF+KVA+NVNCLTL  ASPT EFGIIGNLA ANFLIGYDLEAK
Sbjct: 361 ITAQFSGGANVNLLPVNTFQKVANNVNCLTLTPASPTDEFGIIGNLALANFLIGYDLEAK 420

Query: 421 RLSFKPTVC 430
           RLSFKPTVC
Sbjct: 421 RLSFKPTVC 429

[Close]

BLAST of CSPI04G06730 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.8e-91
Identity = 196/440 (44.55%), Postives = 267/440 (60.68%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSR 65
           SL F  +L L+  S       D GFT  L HRDS  SP   S+ +   R+ NA RRS   
Sbjct: 3   SLIFATLLSLLLLSNVNAYPKD-GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARS 62

Query: 66  SAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLK 125
           +    N  A+  +   QS I    GEYLM++SIGTPPV  L IADTGSDL W QC PC  
Sbjct: 63  TLQFSNDDASPNSP--QSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCED 122

Query: 126 CYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGV-QGVCDYSYTYGDRTYSKGDLGF 185
           CYQQ  P+F+P +S+++  V C++  C A++D  C   +  C Y+ TYGD +Y+KGD+  
Sbjct: 123 CYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAV 182

Query: 186 EKITIGSSSVKSV------IGCGHASSGGFGFA-SGVIGLGGGHLSLVSQMSQTSGISRR 245
           + +T+GSS  + V      IGCGH ++G F  A SG+IGLGGG  SLVSQ+ ++  I+ +
Sbjct: 183 DTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKS--INGK 242

Query: 246 FSYCLPTLLSHA--NGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHM 305
           FSYCL    S      KINFG N +VSG GVVST ++ K+  TYY++ LEAIS+G+++  
Sbjct: 243 FSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQ 302

Query: 306 AFAK-----QGNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGI 365
             +      +GN++IDSGTTLT+LP   Y  + S +   +KA+RV+DP G L LC+ D  
Sbjct: 303 FTSTIFGTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYRD-- 362

Query: 366 NVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQAN 425
             ++S  +P IT HF GG +V L  +NTF  V+++V+C    A    T   I GNLAQ N
Sbjct: 363 --SSSFKVPDITVHFKGG-DVKLGNLNTFVAVSEDVSCFAFAANEQLT---IFGNLAQMN 422

Query: 426 FLIGYDLEAKRLSFKPTVCA 431
           FL+GYD  +  +SFK T C+
Sbjct: 423 FLVGYDTVSGTVSFKKTDCS 429

[Close]

BLAST of CSPI04G06730 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 323.6 bits (828), Expect = 2.5e-88
Identity = 195/451 (43.24%), Postives = 272/451 (60.31%), Query Frame = 0


Query: 11  LILFLISFSQTTIING-DNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRRSLSRSAAL 70
           L+ F + FS T   +G    F+  L HRDS LSP+    ++  DRL  AF RS+SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 71  LNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQ 130
            ++ + +    LQS +    GE+ MS++IGTPP+    IADTGSDLTW QC PC +CY++
Sbjct: 66  NHQLSQT---DLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKE 125

Query: 131 LRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGV---QGVCDYSYTYGDRTYSKGDLGFEK 190
             PIF+  KS+++   PC+++ C A+     G      +C Y Y+YGD+++SKGD+  E 
Sbjct: 126 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 185

Query: 191 ITIGSSSVK------SVIGCGHASSGGFG-FASGVIGLGGGHLSLVSQMSQTSGISRRFS 250
           ++I S+S        +V GCG+ + G F    SG+IGLGGGHLSL+SQ+   S IS++FS
Sbjct: 186 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFS 245

Query: 251 YCLPTLLSHANGK--INFGENAVVSG----PGVVSTPLISKNTVTYYYITLEAISIG--- 310
           YCL    +  NG   IN G N++ S      GVVSTPL+ K  +TYYY+TLEAIS+G   
Sbjct: 246 YCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKK 305

Query: 311 ----------NERHMAFAKQGNVIIDSGTTLTILPKELYDGVVSSLLK-VVKAKRVKDPH 370
                     N+  +     GN+IIDSGTTLT+L    +D   S++ + V  AKRV DP 
Sbjct: 306 IPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQ 365

Query: 371 GSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTE 430
           G L  CF  G   +A +G+P IT H F GA+V L PIN F K+++++ CL++    PTTE
Sbjct: 366 GLLSHCFKSG---SAEIGLPEITVH-FTGADVRLSPINAFVKLSEDMVCLSM---VPTTE 425

[Close]

BLAST of CSPI04G06730 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 311.6 bits (797), Expect = 9.8e-85
Identity = 187/446 (41.93%), Postives = 267/446 (59.87%), Query Frame = 0


Query: 6   SLFFHLILFLISFSQTTIINGDN----GFTTSLFHRDSLLSPLEFSSLSHYDRLANAFRR 65
           SLF  ++L L   S   + N +     GFT  L HRDS  SP      +   RL NA  R
Sbjct: 3   SLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHR 62

Query: 66  SLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQCL 125
           S++R      +  T      Q  +   SGEYLM+VSIGTPP   + IADTGSDL W QC 
Sbjct: 63  SVNRVFHFTEKDNTPQP---QIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 122

Query: 126 PCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDD-GHCGV-QGVCDYSYTYGDRTYSK 185
           PC  CY Q+ P+F+P  S+++  V C++  C A+++   C      C YS +YGD +Y+K
Sbjct: 123 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 182

Query: 186 GDLGFEKITIGSSSVKS------VIGCGHASSGGFG-FASGVIGLGGGHLSLVSQMSQTS 245
           G++  + +T+GSS  +       +IGCGH ++G F    SG++GLGGG +SL+ Q+  + 
Sbjct: 183 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDS- 242

Query: 246 GISRRFSYCLPTLLSHAN--GKINFGENAVVSGPGVVSTPLISK-NTVTYYYITLEAISI 305
            I  +FSYCL  L S  +   KINFG NA+VSG GVVSTPLI+K +  T+YY+TL++IS+
Sbjct: 243 -IDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISV 302

Query: 306 GNERHM-----AFAKQGNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDL 365
           G+++       + + +GN+IIDSGTTLT+LP E Y  +  ++   + A++ +DP   L L
Sbjct: 303 GSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 362

Query: 366 CFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIG 425
           C+    +    L +PVIT H F GA+V L   N F +V++++ C   +  SP+  F I G
Sbjct: 363 CY----SATGDLKVPVITMH-FDGADVKLDSSNAFVQVSEDLVCFAFR-GSPS--FSIYG 422

Query: 426 NLAQANFLIGYDLEAKRLSFKPTVCA 431
           N+AQ NFL+GYD  +K +SFKPT CA
Sbjct: 423 NVAQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of CSPI04G06730 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 302.8 bits (774), Expect = 4.5e-82
Identity = 191/458 (41.70%), Postives = 261/458 (56.99%), Query Frame = 0


Query: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60
           MA    L+  L+     F+  +  N +N  T  L HRDS  SPL     +  DRL  AF 
Sbjct: 1   MATKTFLYCSLLAISFFFASNSSANREN-LTVELIHRDSPHSPLYNPHHTVSDRLNAAFL 60

Query: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120
           RS+SRS     +        LQS +    GEY MS+SIGTPP     IADTGSDLTW QC
Sbjct: 61  RSISRSRRFTTK------TDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQC 120

Query: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGV---QGVCDYSYTYGDRTY 180
            PC +CY+Q  P+F+  KS+++    C+++TC A+ +   G    + +C Y Y+YGD ++
Sbjct: 121 KPCQQCYKQNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSF 180

Query: 181 SKGDLGFEKITIGSSSVKS------VIGCGHASSGGF-GFASGVIGLGGGHLSLVSQMSQ 240
           +KGD+  E I+I SSS  S      V GCG+ + G F    SG+IGLGGG LSLVSQ+  
Sbjct: 181 TKGDVATETISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLG- 240

Query: 241 TSGISRRFSYCLPTLLSHANGK--INFGENAVVSGP----GVVSTPLISKNTVTYYYITL 300
            S I ++FSYCL    +  NG   IN G N++ S P      ++TPLI K+  TYY++TL
Sbjct: 241 -SSIGKKFSYCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTL 300

Query: 301 EAISIGNER-----------HMAFAKQGNVIIDSGTTLTILPKELYDGVVSSLLK-VVKA 360
           EA+++G  +             +  + GN+IIDSGTTLT+L    YD   +++ + V  A
Sbjct: 301 EAVTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGA 360

Query: 361 KRVKDPHGSLDLCFDDGINVAASLGIPVITAHFFGGANVNLLPINTFRKVADNVNCLTLK 420
           KRV DP G L  CF  G      +G+P IT H F  A+V L PIN F K+ ++  CL++ 
Sbjct: 361 KRVSDPQGLLTHCFKSG---DKEIGLPAITMH-FTNADVKLSPINAFVKLNEDTVCLSM- 420

Query: 421 AASPTTEFGIIGNLAQANFLIGYDLEAKRLSFKPTVCA 431
              PTTE  I GN+ Q +FL+GYDLE K +SF+   C+
Sbjct: 421 --IPTTEVAIYGNMVQMDFLVGYDLETKTVSFQRMDCS 442

[Close]

BLAST of CSPI04G06730 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-59
Identity = 144/354 (40.68%), Postives = 196/354 (55.37%), Query Frame = 0


Query: 92  YLMSVSIGTPPVDYLGIADTGSDLTWAQCLPCLKCYQQLRPIFNPLKSTSFSHVPCNTQT 151
           YLM + +GTPP +   I DTGS++TW QCLPC+ CY+Q  PIF+P KS++F    C    
Sbjct: 65  YLMKLQVGTPPFEIQAIIDTGSEITWTQCLPCVHCYEQNAPIFDPSKSSTFKEKRC---- 124

Query: 152 CHAVDDGHCGVQGVCDYSYTYGDRTYSKGDLGFEKITIGSSS------VKSVIGCGHASS 211
                DGH      C Y   Y D TY+ G L  E IT+ S+S       +++IGCGH +S
Sbjct: 125 -----DGHS-----CPYEVDYFDHTYTMGTLATETITLHSTSGEPFVMPETIIGCGHNNS 184

Query: 212 GGFGFASGVIGLGGGHLSLVSQM-SQTSGISRRFSYCLPTLLSHANGKINFGENAVVSGP 271
                 SG++GL  G  SL++QM  +  G+    SYC          KINFG NA+V+G 
Sbjct: 185 WFKPSFSGMVGLNWGPSSLITQMGGEYPGL---MSYCFS---GQGTSKINFGANAIVAGD 244

Query: 272 GVVSTPLISKNTVT-YYYITLEAISIGNERHMAF-----AKQGNVIIDSGTTLTILPKEL 331
           GVVST +        +YY+ L+A+S+GN R         A +GN++IDSGTTLT  P   
Sbjct: 245 GVVSTTMFMTTAKPGFYYLNLDAVSVGNTRIETMGTTFHALEGNIVIDSGTTLTYFPVSY 304

Query: 332 YDGVVSSLLKVVKAKRVKDPHGSLDLCFD-DGINVAASLGIPVITAHFFGGANVNLLPIN 391
            + V  ++  VV A R  DP G+  LC++ D I++      PVIT HF GG ++ L   N
Sbjct: 305 CNLVRQAVEHVVTAVRAADPTGNDMLCYNSDTIDI-----FPVITMHFSGGVDLVLDKYN 364

Query: 392 TFRKVAD-NVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAKRLSFKPTVCA 431
            + +  +  V CL +   SPT E  I GN AQ NFL+GYD  +  +SF PT C+
Sbjct: 365 MYMESNNGGVFCLAIICNSPTQE-AIFGNRAQNNFLVGYDSSSLLVSFSPTNCS 392

The following BLAST results are available for this feature:

BLAST of CSPI04G06730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBM5	3.5e-87	43.24	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q6XBF8	1.4e-83	41.93	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q766C3	7.6e-58	36.56	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.3e-54	36.13	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LHE3	2.0e-50	32.09	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of CSPI04G06730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV20	3.7e-241	99.07	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G05540...	[more]
A0A5A7TSV3	6.8e-219	89.74	Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A5D3CX41	2.0e-218	90.57	Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BUB0	2.0e-218	90.57	probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=...	[more]
A0A5D3CYR2	8.3e-217	89.28	Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of CSPI04G06730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149005.3	2.0e-241	99.30	probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649217.1 hypothetica...	[more]
KAA0044967.1	1.4e-218	89.74	putative aspartic protease [Cucumis melo var. makuwa]	[more]
XP_008452153.1	4.1e-218	90.57	PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putat...	[more]
XP_008452152.1	1.7e-216	89.28	PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putat...	[more]
XP_031739975.1	9.5e-215	88.11	probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetica...	[more]

BLAST of CSPI04G06730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64830.1	1.8e-91	44.55	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	2.5e-88	43.24	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	9.8e-85	41.93	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	4.5e-82	41.70	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	1.9e-59	40.68	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 280..425 e-value: 1.6E-23 score: 83.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 71..257 e-value: 5.1E-47 score: 162.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 262..430 e-value: 7.1E-41 score: 142.0
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 87..429
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 92..256 e-value: 2.1E-46 score: 158.3
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 4..429
None	No IPR available	PANTHER	PTHR47967:SF66	ASPARTIC PROTEINASE CDR1-RELATED	coord: 4..429
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 303..314
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 92..425 score: 38.625294
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 91..429 e-value: 2.87176E-82 score: 251.799

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G06730.1	CSPI04G06730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity