CSPI04G06350 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G06350
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionlong chain acyl-CoA synthetase 4-like
LocationChr4: 4395961 .. 4401909 (-)
RNA-Seq ExpressionCSPI04G06350
SyntenyCSPI04G06350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACATTTTAAAATTTCTAAAGTTAGATATTCCATTCCACGGGTTAGTTTTAGAGAAAGAGAAGGACTAAACACATAAAAAGTACAAAAGTGAGGGACTAAATTTGCAATTTTTTTTACTTGGTGAAGGAAAAGAAAAGGGAAAAGATTCAAAGAAGAAGAAAAAAAGGGTGTATTTACGATGTACGCCACTTGGTCAGGTCACCCATAGCCACCAATATTCATGACTTTTGTACTTTGATTTGTATATATTAAACCAATTTCTTCCTCTCAATTTCATCGATTTTTTTTGAATTTTGAACGAATCCACACTCTCTGTTTCCGGATTTTTACTATCTATCGGTGTCCGGAGGATGTCTCAGATGAAGTATTTGATCGAGGTGGAGAAGTCCAAGTCCGCCATTGATGGAAGGCCTTCGATCGGACCCGTTTACAGAAGCATTTTCGCCAAAGACGGCTTTCCCCTGCCCATTCAAGGATTGGATAGCTGTTGGGATATATTTCGGTTTGATTTCTTCACTTTCTTCTTCTTTGCTGAGAGATACCGCTACTAGATTCTGCATTTTGTGCCTTCTTCTATTCCAGCTTTTATTTTAGCCGAGTACAAGGTGCTTTCCCGTTATGTTTATATCGTTACTTAAGTTGAGTTCTGCTCTGTGTATTCCGCTAGATTTTCTCGTTGTTTTTTATATATATACTGTGCTGATGAATATATTGAAATTTGAGTATGATTAGTTTTGATCAAAAGTTTCATAGTTCATTGTTTTTTTTAGTACTTGGCTCTGCCGCTTTTGATTGGAACAGTTATAGTTCAAACTGCTTAAGATCAATATATATCTTTTTCTCTTTAGATATAAATTACTGTACCATGCCGGGTGGGTGTGTTCTGCTTGCTCTGTTCTTACCGTCATATTTGAGCTTCATCCTTTTTGGCAGTTCCATTTTCCCATTTTCTTGAGCTTTGATCAATACCATTCTTTTGTTTTCTCAGCTTTGCATGGGATTATATTAGACCTTTGCTTGTTGGCTTATGAGTTATTTGATATCCATCGTTAATATGATGATCATCGTTAAATGTAATGCGCCAATTTTTCCATTGATTACTGTTATATTGCAGGTTATCAGTGGAAAGAAACCCTGGTAATCGAATGCTCGGGCACCGTGAGATTGTGAATGGAAAGGTATATGCATCTTGCAAATAATCCAAACGCCCCAAAGTGTATGTTTTTGTTTTTTCTAATATCCAATAAGACCTTGCAATAAGTTGTAGCTTGGTATGTTTTTGTAGGCTGGTAAATATGTCTGGCTAACATATAAGGAAGTGTACGACACTGTAGTAAAAGTTGGAAATTCTTTGCGTACTCGTGGTTTTGAGCAAGTATGCTTCCATTCTCATTAAGATATTCAATTCATTTATGCAAGCACATTCCTGCAAATCTTGTGGATTAAAGCTTATCCTAATCTCCATTTATAGGGAGTAAAATGTGGTATTTATGGTGCCAATTGCTCAGAGTGGATAATAAGCATGGAGGTAGACATTTTCTTTTGCTTCTTACTTCTTTCTTGATATCATTCATCTTTTATCTTCCAGTTTTTTCTTCTATGGAGTTTCAGAAACGTGCTGACTTTCACAGGACTCATTTGTTTTCTGCTATCTTCTCTAATTTCTTGTTACTTCATCCTAGAGAAATATTATATGACACTTAATAATACTTGCAGGCATGTAATGCTCATGGACTATATTGTGTTCCTTTGTATGACACGTTAGGTATGTCCATATGCCTTTTCCTCCAAGTTTTCAATTGGAAGACAGAATTGCACTGTTCCTCTAAATTTATTCTCATATTATGTGGTTTAGTATTTATTTTATTAGAAAATTAAGAGTAAGCTGATTGACTTTTTTATCATATTGATTCAATGTTTAGGTGCGGGTGCTGTGGAATTTATTATTTGCCATTCCGAAATTTCTGTTGCTTTTGTAGAAGAGAAGAAGCTTCCTGAGGTACAGACCTGTGGTGGAAGCCAGAAATTTAGAATCTTTATTTTTTAAGTTACATATATGGTTATTAACTACAGATCTTTACTCTCTGAGAACGGTGATTTGGTGGAACAATATGTAAAAGGTACCTGTCCCGGTTAGTTCTGATCATATGAAATTTATATTTTTTTTCCAGGTCTTGAAAACATTTCCAAGCACAACCAAATACTTGAAAAGTGAGTTTTTATTACTATTTAGATTTTTTTTTCCAATGATGTGTCTGTAGAAGCACATTTTTATTTATTTAGTTCTATTTTTATTAAAATGTTTCATTTGTTTGTTTGTAGCAATTGTGAGTTTTGGCAAGGTTACACCTAATCTGAGAGAGGAGTTCGAGAAGCATGGGGTTGAAATATTTTCTTGGGACGAATTTTTGCTTCTGGTAAGTTTTGATCCTCGATAGGTCTCTTTTATTCAGTAATTTAGTCTTTAGTTTGAAAATTTTCTTCATCTCAACTTAATGAAATTATCCATAGTAACATTTTGTTTGGGAATATGCTGTTTCCTCTTCTGAGTATTGACAATGAACTGAAAACTTTTCTATTAAGATAATATTGATATGTGTAAAGATATGTCGTAGACCTGGTAATAGCCTTTTTTTTACCCTTCATCTTTCATCATGAGGAAGAAAGTACAAATTGCTCCCATCAACTTTTAAGCTGTCATTTATTGTATCTAATCTTTTTGGTATCTGCTTAAAACAGGGTGAAAATCAACATTTTGATCTTCCTGTGAAAAAGAAAAGTGATATTTCCACTATTATGTATACAAGTGGAACTACTGGAGATCCTAAAGGAGTCATGATATCGAACAAAAGCATAATTTCACTTATAGCTGGTGTCCAGCGGCTCTTAGAGAATGTAAATGAAGAGGTAGTTTTCCTTAGTTTTTTGTACGTCCAGTGTTTTCTTTACGAAAGCCTTGAAATGGAAATTTGAATAATATACCCTATGAAATAGTCAATCTAGATTGATAGTCATTGTATCTTTTGTGTCTAATTGCTCGATTTCTTACGTCTCTTCACTCTCTCCCTCCCTCTCTCCCTCATACAGTTGAGTCCAAAAGATGTATATTTGTCATACCTTCCGCTAGCACATATCTTTGACCGAGTGATCGAGGAGCTGTTCATCTTACATGGTGCTTCCATTGGGTTCTGGCGTGGGGTGAGTTCTCATTTGATTTTCTTTGTGTGCCAATTATAAGGTTTATAAAATATGTTTTTACATTCTGCAGGATGTTAAATTAATCATTGAAGATATTGGGGAGCTCAAACCCAGTATTTTCTGTGCCGTTCCCCGAGTTTTAGATAGAATTTATGCTGGTAAGCTGATCGTATGTGAAATATAATATTTTGCCTAATAATTATTATTGAGAACAAGCATCTCTAAAGCTAGAACCAGTTATGTGAATTGAAGTTGTCATTATCCAGCGTGTTGCTCTGGTTTTATCGCTTTGGTCTGTGGATACTACTGTTTTGATTTGGGTGCAGCAAGTTTATATCTCCCATGATCAAAATAGTAATTATTATATTTTCTCAGGCTTGACTCAGAGGTTGGATCAAGGGGGGCTCTTAAAGAGGACATTATTCAATGTTGCATTCTCACGGTGAGTTAGTTCCACTTTGGTTTAATTTTCTTAAAATATGTATTGTATTAGTGCAGATGAATTTTGTTAAATAAAACAAAGAAAGTTTCATAAAATCTCGTTCTATTCCTTGTAGGAAGATTTTTATAAGTAAAACTACTTAACATGAGCGAATGTATTTCATGACGAGTGATACTTTTATGCATAAACTCAACTATGTTAGTCATGGTTCACTAACTGCCTTAGAACTTGACAATAGTTCGGTCAACTTAGTGATCATAGACATTAGACAAGGTGTTCCTGGGGTATTTTTGTGGATTTGAACTTTCAGTAGTTCTTGTCCATTATTTCACTTATCGTTAATCTCATTAGTCTTACTCTTTATTGCAGTAAATATGGTAACATGCAAAAGGGGCATAAACATAGCGAGGCATCTCCAATTTGTGACAGATTTGTCTTTAATAAGGTGAAAAACATTTTCTTCGACACACCTTCTATATTTGACATGAACCTCAGATTTACTAAAATTTTAATCCATATATTTTGTCCTTTTGGGGTTCTTGACCAGGTAAAGGAAGGGTTGGGAGGTAATGTGAGACTTATTCTATCTGGAGCCGCACCTCTAGCTACCCACGTAGAAGCTTTCTTAAGAGTTGTGGCATGTGCACATGTTCTACAAGGATATGGTATGTAATCTCTGCAGTCATTCTCAGTTTTCTACTCTTCATTGACTCGTATAGTTTCTTATAATTTTTTACCATTCCTTCCATTTCTGTTTCACCCATACTTCAATGATGATTATTTCATGCTAATGCAGGTTTAACAGAGACCTGTGCTGGGACTTTTGTCTCATTGCCAAATGAACTTTCAATGTTGGGAACAGTTGGCCCTCCAGTGCCAAATGTGGATGTCTGCCTAGAATCTGTCCCCGAAATGGGATATGATGCCCTTTCGACCACACCACGTGGTGAAATATGTGTTAGGGGAGACCCATTGTTTTCTGGATACTACAAGCGTGATGACTTAACCAATGAGGTCATGATTGATGGATGGTTCCATACAGGTAATCTTAATCCGGTTTGCCATAAAATTTATTTGCTCGTTTTTCTATGGTTCGCTATCATAAGTTTACCTCCTATTTTTCTTCTTTTCTTTAAGGTGATATTGGTGAGTGGCAATCGGATGGGAGCTTGAAAATTATTGACCGGAAGAAGAATATTTTCAAGCTTTCTCAAGGAGAATACGTTGCCGTGGAAAATTTGGAGAATATTTATGGGCTTGTATCTTCTATAGATATGGTATTTTCTAAACTTTTGTTAGCTTTTAATAGCAATGTTTTTTATGCACATTACTGACATTAGAAAAGAAAGTTTGAGTTATTTATACATAATTTTCTGATGCTCAATTTAGATCTGGGTCTACGGGAACAGTTTCGAGTCGTTTCTAGTTGCAGTTGCTAATCCCAACCAGCTAGCACTTGAACGTTGGGCAAACGAAAACGGTATCGAAGGGGACTTTGATTCACTTTGTGGAGATAAGAGGGCAAAGGACTACCTGCTTGGGGAGCTCAACAAAATTGCAAAAGAGAAGAAGGTTTTATTTCTCCCAATTTGCTGCACACTTCTTTTGATTTGACTAAACAAATATTTTACTCAATTGTCAAAAACTAACATGAAGCTCTCACAACTCTTGTTCTTGGCAGTTGAAAGGCTTTGAGTTTATTAAAGCCATTCACCTCGACCCTCTACCATTTGATATGGAACGTGACCTAATAACTCCAACGTACAAGAAGAAGAGACCTCAATTGCTCAAATATTATCAGGTTCATGCTCAAATAACAACCTATCTCATCTATCAATTTAGTCAAATTTCCCAAACTCGTCAAACTTATTTTATGACTTCAAAATCTTAGAAATAAAGGATTAGAACTCAATTGTAATTAAACTAAAGGGTACTCAATCCAAATACATGATTGTTTGTGCTTTCATTTTTCTCTCATTGGTAAAGATTTTCATTCAATTTTACTGCGTGATACTGATGAACGATTTATTTTTCTTTGGCAGAGTGTAATTGACAGCATGTACAATAATGCAAAGAAGCCTTAATGAATGAAGATGACATTTATGGAATCCATTCCAAAACTTGCACTCTTTCTCATGAAATGATCCCCTTTTTCTTTTACTGTACATATGGGTTACTAATCAACCTAATCAATCCATTTATGTGAATGATGAAGCTTTTGTTTTAGAGGGACATAGACATTGTTGAACCAAACATTTCTGCTATATTAAATCTAGTTATTAGTTGTATCATTATAGACACGTTAGC

mRNA sequence

TCACATTTTAAAATTTCTAAAGTTAGATATTCCATTCCACGGGTTAGTTTTAGAGAAAGAGAAGGACTAAACACATAAAAAGTACAAAAGTGAGGGACTAAATTTGCAATTTTTTTTACTTGGTGAAGGAAAAGAAAAGGGAAAAGATTCAAAGAAGAAGAAAAAAAGGGTGTATTTACGATGTACGCCACTTGGTCAGGTCACCCATAGCCACCAATATTCATGACTTTTGTACTTTGATTTGTATATATTAAACCAATTTCTTCCTCTCAATTTCATCGATTTTTTTTGAATTTTGAACGAATCCACACTCTCTGTTTCCGGATTTTTACTATCTATCGGTGTCCGGAGGATGTCTCAGATGAAGTATTTGATCGAGGTGGAGAAGTCCAAGTCCGCCATTGATGGAAGGCCTTCGATCGGACCCGTTTACAGAAGCATTTTCGCCAAAGACGGCTTTCCCCTGCCCATTCAAGGATTGGATAGCTGTTGGGATATATTTCGGTTATCAGTGGAAAGAAACCCTGGTAATCGAATGCTCGGGCACCGTGAGATTGTGAATGGAAAGGCTGGTAAATATGTCTGGCTAACATATAAGGAAGTGTACGACACTGTAGTAAAAGTTGGAAATTCTTTGCGTACTCGTGGTTTTGAGCAAGGAGTAAAATGTGGTATTTATGGTGCCAATTGCTCAGAGTGGATAATAAGCATGGAGGCATGTAATGCTCATGGACTATATTGTGTTCCTTTGTATGACACGTTAGGTGCGGGTGCTGTGGAATTTATTATTTGCCATTCCGAAATTTCTGTTGCTTTTGTAGAAGAGAAGAAGCTTCCTGAGGTCTTGAAAACATTTCCAAGCACAACCAAATACTTGAAAACAATTGTGAGTTTTGGCAAGGTTACACCTAATCTGAGAGAGGAGTTCGAGAAGCATGGGGTTGAAATATTTTCTTGGGACGAATTTTTGCTTCTGGGTGAAAATCAACATTTTGATCTTCCTGTGAAAAAGAAAAGTGATATTTCCACTATTATGTATACAAGTGGAACTACTGGAGATCCTAAAGGAGTCATGATATCGAACAAAAGCATAATTTCACTTATAGCTGGTGTCCAGCGGCTCTTAGAGAATGTAAATGAAGAGTTGAGTCCAAAAGATGTATATTTGTCATACCTTCCGCTAGCACATATCTTTGACCGAGTGATCGAGGAGCTGTTCATCTTACATGGTGCTTCCATTGGGTTCTGGCGTGGGGATGTTAAATTAATCATTGAAGATATTGGGGAGCTCAAACCCAGTATTTTCTGTGCCGTTCCCCGAGTTTTAGATAGAATTTATGCTGGCTTGACTCAGAGGTTGGATCAAGGGGGGCTCTTAAAGAGGACATTATTCAATGTTGCATTCTCACGTAAATATGGTAACATGCAAAAGGGGCATAAACATAGCGAGGCATCTCCAATTTGTGACAGATTTGTCTTTAATAAGGTAAAGGAAGGGTTGGGAGGTAATGTGAGACTTATTCTATCTGGAGCCGCACCTCTAGCTACCCACGTAGAAGCTTTCTTAAGAGTTGTGGCATGTGCACATGTTCTACAAGGATATGGTTTAACAGAGACCTGTGCTGGGACTTTTGTCTCATTGCCAAATGAACTTTCAATGTTGGGAACAGTTGGCCCTCCAGTGCCAAATGTGGATGTCTGCCTAGAATCTGTCCCCGAAATGGGATATGATGCCCTTTCGACCACACCACGTGGTGAAATATGTGTTAGGGGAGACCCATTGTTTTCTGGATACTACAAGCGTGATGACTTAACCAATGAGGTCATGATTGATGGATGGTTCCATACAGGTGATATTGGTGAGTGGCAATCGGATGGGAGCTTGAAAATTATTGACCGGAAGAAGAATATTTTCAAGCTTTCTCAAGGAGAATACGTTGCCGTGGAAAATTTGGAGAATATTTATGGGCTTGTATCTTCTATAGATATGATCTGGGTCTACGGGAACAGTTTCGAGTCGTTTCTAGTTGCAGTTGCTAATCCCAACCAGCTAGCACTTGAACGTTGGGCAAACGAAAACGGTATCGAAGGGGACTTTGATTCACTTTGTGGAGATAAGAGGGCAAAGGACTACCTGCTTGGGGAGCTCAACAAAATTGCAAAAGAGAAGAAGTTGAAAGGCTTTGAGTTTATTAAAGCCATTCACCTCGACCCTCTACCATTTGATATGGAACGTGACCTAATAACTCCAACGTACAAGAAGAAGAGACCTCAATTGCTCAAATATTATCAGAGTGTAATTGACAGCATGTACAATAATGCAAAGAAGCCTTAATGAATGAAGATGACATTTATGGAATCCATTCCAAAACTTGCACTCTTTCTCATGAAATGATCCCCTTTTTCTTTTACTGTACATATGGGTTACTAATCAACCTAATCAATCCATTTATGTGAATGATGAAGCTTTTGTTTTAGAGGGACATAGACATTGTTGAACCAAACATTTCTGCTATATTAAATCTAGTTATTAGTTGTATCATTATAGACACGTTAGC

Coding sequence (CDS)

ATGTCTCAGATGAAGTATTTGATCGAGGTGGAGAAGTCCAAGTCCGCCATTGATGGAAGGCCTTCGATCGGACCCGTTTACAGAAGCATTTTCGCCAAAGACGGCTTTCCCCTGCCCATTCAAGGATTGGATAGCTGTTGGGATATATTTCGGTTATCAGTGGAAAGAAACCCTGGTAATCGAATGCTCGGGCACCGTGAGATTGTGAATGGAAAGGCTGGTAAATATGTCTGGCTAACATATAAGGAAGTGTACGACACTGTAGTAAAAGTTGGAAATTCTTTGCGTACTCGTGGTTTTGAGCAAGGAGTAAAATGTGGTATTTATGGTGCCAATTGCTCAGAGTGGATAATAAGCATGGAGGCATGTAATGCTCATGGACTATATTGTGTTCCTTTGTATGACACGTTAGGTGCGGGTGCTGTGGAATTTATTATTTGCCATTCCGAAATTTCTGTTGCTTTTGTAGAAGAGAAGAAGCTTCCTGAGGTCTTGAAAACATTTCCAAGCACAACCAAATACTTGAAAACAATTGTGAGTTTTGGCAAGGTTACACCTAATCTGAGAGAGGAGTTCGAGAAGCATGGGGTTGAAATATTTTCTTGGGACGAATTTTTGCTTCTGGGTGAAAATCAACATTTTGATCTTCCTGTGAAAAAGAAAAGTGATATTTCCACTATTATGTATACAAGTGGAACTACTGGAGATCCTAAAGGAGTCATGATATCGAACAAAAGCATAATTTCACTTATAGCTGGTGTCCAGCGGCTCTTAGAGAATGTAAATGAAGAGTTGAGTCCAAAAGATGTATATTTGTCATACCTTCCGCTAGCACATATCTTTGACCGAGTGATCGAGGAGCTGTTCATCTTACATGGTGCTTCCATTGGGTTCTGGCGTGGGGATGTTAAATTAATCATTGAAGATATTGGGGAGCTCAAACCCAGTATTTTCTGTGCCGTTCCCCGAGTTTTAGATAGAATTTATGCTGGCTTGACTCAGAGGTTGGATCAAGGGGGGCTCTTAAAGAGGACATTATTCAATGTTGCATTCTCACGTAAATATGGTAACATGCAAAAGGGGCATAAACATAGCGAGGCATCTCCAATTTGTGACAGATTTGTCTTTAATAAGGTAAAGGAAGGGTTGGGAGGTAATGTGAGACTTATTCTATCTGGAGCCGCACCTCTAGCTACCCACGTAGAAGCTTTCTTAAGAGTTGTGGCATGTGCACATGTTCTACAAGGATATGGTTTAACAGAGACCTGTGCTGGGACTTTTGTCTCATTGCCAAATGAACTTTCAATGTTGGGAACAGTTGGCCCTCCAGTGCCAAATGTGGATGTCTGCCTAGAATCTGTCCCCGAAATGGGATATGATGCCCTTTCGACCACACCACGTGGTGAAATATGTGTTAGGGGAGACCCATTGTTTTCTGGATACTACAAGCGTGATGACTTAACCAATGAGGTCATGATTGATGGATGGTTCCATACAGGTGATATTGGTGAGTGGCAATCGGATGGGAGCTTGAAAATTATTGACCGGAAGAAGAATATTTTCAAGCTTTCTCAAGGAGAATACGTTGCCGTGGAAAATTTGGAGAATATTTATGGGCTTGTATCTTCTATAGATATGATCTGGGTCTACGGGAACAGTTTCGAGTCGTTTCTAGTTGCAGTTGCTAATCCCAACCAGCTAGCACTTGAACGTTGGGCAAACGAAAACGGTATCGAAGGGGACTTTGATTCACTTTGTGGAGATAAGAGGGCAAAGGACTACCTGCTTGGGGAGCTCAACAAAATTGCAAAAGAGAAGAAGTTGAAAGGCTTTGAGTTTATTAAAGCCATTCACCTCGACCCTCTACCATTTGATATGGAACGTGACCTAATAACTCCAACGTACAAGAAGAAGAGACCTCAATTGCTCAAATATTATCAGAGTGTAATTGACAGCATGTACAATAATGCAAAGAAGCCTTAA

Protein sequence

MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNKIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP*
Homology
BLAST of CSPI04G06350 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 1.7e-303
Identity = 487/658 (74.01%), Postives = 575/658 (87.39%), Query Frame = 0

Query: 3   QMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRM 62
           Q KY+ +VE+ K   DGRPS+GPVYRSIFAKDGFP PI+G+DSCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEA 122
           LG REIV+GK GKYVW TY+EVYD V+K+GNSLR+ G +   KCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII HSE+S+ FVEEKK+ E+ KT P++T+Y+KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E  G+ I++WDEFL LGE + +DLP+KKKSDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL++ NE L+ KDVYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGH 362
           VKL+IED+ ELKP+IFCAVPRVLDR+Y+GL ++L  GG LK+ +F+ AFS K+G M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 KHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTET 422
            H EASP+ D+ VF+KVK+GLGGNVR+ILSGAAPLA+HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVS 542
           KR+DLT EV+IDGW HTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNKI 602
           ++D +WVYGNSFESFL+A+ANPNQ  LERWA ENG+ GD+D+LC +++AK+++LGEL K+
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYN--NAK 659
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQSVID MY   NAK
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of CSPI04G06350 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 3.2e-297
Identity = 475/658 (72.19%), Postives = 554/658 (84.19%), Query Frame = 0

Query: 2   SQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNR 61
           SQ +++ EVE +K A DG PS+GPVYRS FA++GFP PI G+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISME 121
           MLG REI NGKAGKYVW TYKEVYD V+K+GNSLR+ G ++G KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII H+E+S+AFVEEKK+PE+ KT P++TKY+KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK G+ I SWDEFL LGE + ++LP+KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L NVN  LS KDVY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKG 361
           DVKL+IED+GELKPSIFCAVPRVLDR+Y GL Q+L  GG  K+ +F+VAFS K+GNM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTE 421
             H  ASP CD+ VFNKVK+GLGGNVR+ILSGAAPLA+H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLV 541
           YKR+DLT EV IDGW HTGD+GEWQ +GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y  V
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNK 601
             I+ IWVYGNSFESFLVA+ANP Q  LERWA ENG+ GDF+S+C + +AK ++LGEL K
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQ+VI  MY   K+
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 660

BLAST of CSPI04G06350 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 8.9e-292
Identity = 465/660 (70.45%), Postives = 561/660 (85.00%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAID-GRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPG 60
           M+  +Y++EVEK K  +D G PS+GPVYRSI+AKDGFP P   L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIIS 120
           N MLG REIV+GKAGKYVW TYKEV++ V+K+GNS+RT G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICH+E+S+AF EE K+ E+LKT P +TKYLK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E+H + I+SWD+FL LGE +H++LP K++SD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL+ ++EEL+ KDVYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQ 360
           RGDVK++IEDI  LKP++FCAVPRVL+RIY GL Q+L  GG +K+ LFN AF  K+ NM+
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI D+ VF KVKEGLGGNVRLILSGAAPLA H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGD+GEWQ DG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 LVSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGEL 600
            V++I+ IWVYGNS+ES+LVAV  P+++ +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NKIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
           N++AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  ID MY   ++
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of CSPI04G06350 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.6e-224
Identity = 359/656 (54.73%), Postives = 485/656 (73.93%), Query Frame = 0

Query: 4   MKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRML 63
           M Y ++VE+++ A +  PS GPVYRSI+A+DG     +GL S W+    SV+R+P   ML
Sbjct: 1   MSYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPML 60

Query: 64  GHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEAC 123
           G R+I + +AG YVWLTY+EV+D  +++ +++R+RG   G +CGIYG NC +WI++M+AC
Sbjct: 61  GRRQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQAC 120

Query: 124 NAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFGK 183
            +H +  VPLYDTLG  AVEFII H E+S+AFV+E K+  +L   P+ +  LKTIVSFG 
Sbjct: 121 YSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGN 180

Query: 184 VTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMIS 243
           +T   ++E E  GV  FSW+EF  LG N   +LP K ++DI T+MYTSG TG+PKGV+++
Sbjct: 181 ITSVQKKETEALGVSCFSWEEFSQLG-NLSGELPEKHRTDICTLMYTSGATGEPKGVILT 240

Query: 244 NKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDV 303
           N++I++ I     +LE  ++  S +DVY SYLPLAH++D+++E   I  G++IG+WRGD+
Sbjct: 241 NEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDI 300

Query: 304 KLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGHK 363
           + +++D+ ELKP+IFC VPRV DRIYAG+  ++  GG LK+ LF  A++ K  NM+KG  
Sbjct: 301 RFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLP 360

Query: 364 HSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETC 423
           H +A+P+ D+  F+K+K+G GG +RL+ SGAAPL  HVE +LRV +CA + QGYGLTE+C
Sbjct: 361 HGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESC 420

Query: 424 AGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYK 483
            G   S+ N   M+GTVG P+  ++  LESVPEMGYDALS  PRGEIC+RG  LFSGY+K
Sbjct: 421 GGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHK 480

Query: 484 RDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSS 543
           R+DLT +V++DGWFHTGDIGEWQ +G++KIIDRKKNIFKLSQGEYV VEN+E IY     
Sbjct: 481 REDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPL 540

Query: 544 IDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNKIA 603
           I  IWVYGNSFESFLVAV  P +LALE WA    +  DF SLC + +A  Y+L ELN  A
Sbjct: 541 IASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTA 600

Query: 604 KEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
           K+ +L+GFE +KA++L+P PFDMERDLITPT+K KRPQLLKYY+  +D +Y+ AK+
Sbjct: 601 KKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKE 655

BLAST of CSPI04G06350 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 5.5e-217
Identity = 351/652 (53.83%), Postives = 474/652 (72.70%), Query Frame = 0

Query: 10  VEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRMLGHREIV 69
           VE+ + A    PS GPVYR  +AKDG       +DS W  F  +V++ P  +MLG R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 70  NGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEACNAHGLY 129
           + K G Y W+TYKE +D  +++G+++R+RG + G  CGIYGANC EWII+MEAC + G+ 
Sbjct: 71  DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQGIT 130

Query: 130 CVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFGKVTPNLR 189
            VPLYD+LG  AVEFII H+E+S+ FV+EK +  +L      +  LKTIVSFG+V+   +
Sbjct: 131 YVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQK 190

Query: 190 EEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNKSIIS 249
           EE +   V +FSW+EF L+G     +LP K+K+DI TIMYTSGTTG+PKGV+++N +I  
Sbjct: 191 EEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAAISV 250

Query: 250 LIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLIIED 309
            +  + ++LE  +      DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ +++D
Sbjct: 251 QVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYLMDD 310

Query: 310 IGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGHKHSEASP 369
           +  LKP++FC VPRV D++YAG+ Q++   GL+++ LF+ A++ K GNM+KG    EASP
Sbjct: 311 VQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEEASP 370

Query: 370 ICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAGTFVS 429
             DR +F+K+KE LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+F +
Sbjct: 371 RLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGSFTT 430

Query: 430 LPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRDDLT 489
           L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR DLT
Sbjct: 431 LAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQDLT 490

Query: 490 NEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSIDMIW 549
           ++V+IDGWFHTGDIGEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y     I  IW
Sbjct: 491 DQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIAQIW 550

Query: 550 VYGNSFESFLVAVANPNQLALERWANEN-GIEGDFDSLCGDKRAKDYLLGELNKIAKEKK 609
           VYGNSFESFLV V  P++ A+E WA  N     DF+SLC + +A+ Y L ELN  AK+ +
Sbjct: 551 VYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAKQYQ 610

Query: 610 LKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
           LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+ ++D +Y+ AK+
Sbjct: 611 LKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKR 662

BLAST of CSPI04G06350 vs. ExPASy TrEMBL
Match: A0A0A0KV21 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051550 PE=4 SV=1)

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 660/660 (100.00%), Postives = 660/660 (100.00%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660

BLAST of CSPI04G06350 vs. ExPASy TrEMBL
Match: A0A1S3BTT7 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103493094 PE=4 SV=1)

HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 646/660 (97.88%), Postives = 650/660 (98.48%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AI GRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660

BLAST of CSPI04G06350 vs. ExPASy TrEMBL
Match: A0A5A7TP41 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001980 PE=4 SV=1)

HSP 1 Score: 1303.9 bits (3373), Expect = 0.0e+00
Identity = 635/649 (97.84%), Postives = 639/649 (98.46%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AIDGRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIDGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSV 650
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ +
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQGM 649

BLAST of CSPI04G06350 vs. ExPASy TrEMBL
Match: A0A5D3D1K3 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003860 PE=4 SV=1)

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 633/649 (97.53%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AI GRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSV 650
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ +
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQGM 649

BLAST of CSPI04G06350 vs. ExPASy TrEMBL
Match: A0A6J1J803 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483406 PE=4 SV=1)

HSP 1 Score: 1284.6 bits (3323), Expect = 0.0e+00
Identity = 614/660 (93.03%), Postives = 645/660 (97.73%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLI+VE+SK+AIDGRPSIGPVYRSIFAKDGFPLPI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG REIVNGKAGKYVWLTYKEVYD V+K+GNSLR+RGFE+G KCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EISV FVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEK+G+EIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE++ KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKL++EDIGELKPSIFCAVPRVLDRIYAGLTQRLD GG  ++TLFN+A+S KYGNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKH EASPICD+ VF+KVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN+LALERWA ENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWAKENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY++VIDSMY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of CSPI04G06350 vs. NCBI nr
Match: XP_004149746.1 (long chain acyl-CoA synthetase 4 [Cucumis sativus] >KGN53403.1 hypothetical protein Csa_014409 [Cucumis sativus])

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 660/660 (100.00%), Postives = 660/660 (100.00%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660

BLAST of CSPI04G06350 vs. NCBI nr
Match: XP_008451943.1 (PREDICTED: long chain acyl-CoA synthetase 4-like [Cucumis melo] >XP_008451944.1 PREDICTED: long chain acyl-CoA synthetase 4-like [Cucumis melo])

HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 646/660 (97.88%), Postives = 650/660 (98.48%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AI GRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660

BLAST of CSPI04G06350 vs. NCBI nr
Match: KAA0044904.1 (long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1303.9 bits (3373), Expect = 0.0e+00
Identity = 635/649 (97.84%), Postives = 639/649 (98.46%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AIDGRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIDGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSV 650
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ +
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQGM 649

BLAST of CSPI04G06350 vs. NCBI nr
Match: XP_038876793.1 (long chain acyl-CoA synthetase 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 628/660 (95.15%), Postives = 645/660 (97.73%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K+AIDGRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKPKAAIDGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLGHREIV+GK GKYVWLTYKEVYD VVK+GNSL TRGFE+G KCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVDGKPGKYVWLTYKEVYDIVVKIGNSLHTRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEK+GVEIFSWDEF LLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGVEIFSWDEFSLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEELSP+DVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEELSPEDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKL++EDIGELKPSIFCAVPRVLDRIYAGLTQRLD GG+ K+TLFN+A+SRK+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGIFKKTLFNIAYSRKFGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKH EASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST PRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTIPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIE DFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEEDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ+VID MYNNAKKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNAKKP 660

BLAST of CSPI04G06350 vs. NCBI nr
Match: TYK16566.1 (long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1300.0 bits (3363), Expect = 0.0e+00
Identity = 633/649 (97.53%), Postives = 638/649 (98.31%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGN 60
           MSQMKYLIEVEK K AI GRPSIGPVYRSIFAKDGFP PIQGLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISM 120
           RMLG RE VNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQG KCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKHGVEIFSWDEF LLGENQHFDLPVKK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQK 360
           GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFN+A+SRKYGNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN+LALERWANENGIEGDFDSLC DKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSV 650
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ +
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQGM 649

BLAST of CSPI04G06350 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1042.7 bits (2695), Expect = 1.2e-304
Identity = 487/658 (74.01%), Postives = 575/658 (87.39%), Query Frame = 0

Query: 3   QMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRM 62
           Q KY+ +VE+ K   DGRPS+GPVYRSIFAKDGFP PI+G+DSCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEA 122
           LG REIV+GK GKYVW TY+EVYD V+K+GNSLR+ G +   KCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII HSE+S+ FVEEKK+ E+ KT P++T+Y+KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E  G+ I++WDEFL LGE + +DLP+KKKSDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL++ NE L+ KDVYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGH 362
           VKL+IED+ ELKP+IFCAVPRVLDR+Y+GL ++L  GG LK+ +F+ AFS K+G M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 KHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTET 422
            H EASP+ D+ VF+KVK+GLGGNVR+ILSGAAPLA+HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVS 542
           KR+DLT EV+IDGW HTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNKI 602
           ++D +WVYGNSFESFL+A+ANPNQ  LERWA ENG+ GD+D+LC +++AK+++LGEL K+
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYN--NAK 659
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQSVID MY   NAK
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINAK 661

BLAST of CSPI04G06350 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1021.9 bits (2641), Expect = 2.3e-298
Identity = 475/658 (72.19%), Postives = 554/658 (84.19%), Query Frame = 0

Query: 2   SQMKYLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNR 61
           SQ +++ EVE +K A DG PS+GPVYRS FA++GFP PI G+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISME 121
           MLG REI NGKAGKYVW TYKEVYD V+K+GNSLR+ G ++G KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII H+E+S+AFVEEKK+PE+ KT P++TKY+KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK G+ I SWDEFL LGE + ++LP+KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L NVN  LS KDVY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKG 361
           DVKL+IED+GELKPSIFCAVPRVLDR+Y GL Q+L  GG  K+ +F+VAFS K+GNM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTE 421
             H  ASP CD+ VFNKVK+GLGGNVR+ILSGAAPLA+H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLV 541
           YKR+DLT EV IDGW HTGD+GEWQ +GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y  V
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNK 601
             I+ IWVYGNSFESFLVA+ANP Q  LERWA ENG+ GDF+S+C + +AK ++LGEL K
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQ+VI  MY   K+
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 660

BLAST of CSPI04G06350 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1003.8 bits (2594), Expect = 6.3e-293
Identity = 465/660 (70.45%), Postives = 561/660 (85.00%), Query Frame = 0

Query: 1   MSQMKYLIEVEKSKSAID-GRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPG 60
           M+  +Y++EVEK K  +D G PS+GPVYRSI+AKDGFP P   L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGHREIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIIS 120
           N MLG REIV+GKAGKYVW TYKEV++ V+K+GNS+RT G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICH+E+S+AF EE K+ E+LKT P +TKYLK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E+H + I+SWD+FL LGE +H++LP K++SD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL+ ++EEL+ KDVYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQ 360
           RGDVK++IEDI  LKP++FCAVPRVL+RIY GL Q+L  GG +K+ LFN AF  K+ NM+
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI D+ VF KVKEGLGGNVRLILSGAAPLA H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGD+GEWQ DG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 LVSSIDMIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGEL 600
            V++I+ IWVYGNS+ES+LVAV  P+++ +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NKIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
           N++AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  ID MY   ++
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of CSPI04G06350 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 755.4 bits (1949), Expect = 3.9e-218
Identity = 351/652 (53.83%), Postives = 474/652 (72.70%), Query Frame = 0

Query: 10  VEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRMLGHREIV 69
           VE+ + A    PS GPVYR  +AKDG       +DS W  F  +V++ P  +MLG R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 70  NGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEACNAHGLY 129
           + K G Y W+TYKE +D  +++G+++R+RG + G  CGIYGANC EWII+MEAC + G+ 
Sbjct: 71  DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQGIT 130

Query: 130 CVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFGKVTPNLR 189
            VPLYD+LG  AVEFII H+E+S+ FV+EK +  +L      +  LKTIVSFG+V+   +
Sbjct: 131 YVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQK 190

Query: 190 EEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNKSIIS 249
           EE +   V +FSW+EF L+G     +LP K+K+DI TIMYTSGTTG+PKGV+++N +I  
Sbjct: 191 EEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAAISV 250

Query: 250 LIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLIIED 309
            +  + ++LE  +      DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ +++D
Sbjct: 251 QVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYLMDD 310

Query: 310 IGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGHKHSEASP 369
           +  LKP++FC VPRV D++YAG+ Q++   GL+++ LF+ A++ K GNM+KG    EASP
Sbjct: 311 VQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEEASP 370

Query: 370 ICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAGTFVS 429
             DR +F+K+KE LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+F +
Sbjct: 371 RLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGSFTT 430

Query: 430 LPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRDDLT 489
           L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR DLT
Sbjct: 431 LAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQDLT 490

Query: 490 NEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSIDMIW 549
           ++V+IDGWFHTGDIGEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y     I  IW
Sbjct: 491 DQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIAQIW 550

Query: 550 VYGNSFESFLVAVANPNQLALERWANEN-GIEGDFDSLCGDKRAKDYLLGELNKIAKEKK 609
           VYGNSFESFLV V  P++ A+E WA  N     DF+SLC + +A+ Y L ELN  AK+ +
Sbjct: 551 VYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAKQYQ 610

Query: 610 LKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKK 660
           LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+ ++D +Y+ AK+
Sbjct: 611 LKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKR 662

BLAST of CSPI04G06350 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 673.3 bits (1736), Expect = 2.0e-193
Identity = 318/649 (49.00%), Postives = 443/649 (68.26%), Query Frame = 0

Query: 6   YLIEVEKSKSAIDGRPSIGPVYRSIFAKDGFPLPIQGLDSCWDIFRLSVERNPGNRMLGH 65
           +  +VE+    IDG+PS+GPVYR++ ++ GFP     + + WDIF  SVE+ P N MLG 
Sbjct: 4   FAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLGW 63

Query: 66  REIVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGVKCGIYGANCSEWIISMEACNA 125
           R IV+ K G Y+W TYKEVY+ V+++G++LR  G E G + GIYG NC +WII+MEAC A
Sbjct: 64  RRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEACAA 123

Query: 126 HGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVSFGKVT 185
           H L CVPLYDTLG+GAV++I+ H+EI   FV++ K+  +L+      K LK IVSF  V+
Sbjct: 124 HTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFTNVS 183

Query: 186 PNLREEFEKHGVEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNK 245
             L  +  + GV+ +SW +FL +G  +  D    K  +I TIMYTSGT+GDPKGV+++++
Sbjct: 184 DELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVLTHQ 243

Query: 246 SIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKL 305
           ++ + + G+   ++   ++++  DVYLS+LPLAHI DR+ EE F   GAS+G++ G++ +
Sbjct: 244 AVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGNLNV 303

Query: 306 IIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNVAFSRKYGNMQKGHKHS 365
           + +DI ELKP+    VPRV +RI+ G+ + L +    +R +FN  +  K   + +G+ HS
Sbjct: 304 LRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGYSHS 363

Query: 366 EASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAG 425
           +ASP+ D   F K+++ LGG +RL++SG APL+  +E FLRV  C  V+QGYGLTET  G
Sbjct: 364 KASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTETLGG 423

Query: 426 TFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYKRD 485
           T +  P+E+ MLGTVG P    ++ LE V EMGYD L   P GEIC+RG  +FSGYYK  
Sbjct: 424 TALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYYKNP 483

Query: 486 DLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSID 545
           +LT EVM DGWFHTGDIGE   +G LKIIDRKKN+ KLSQGEYVA+E+LENI+G  S + 
Sbjct: 484 ELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSVVQ 543

Query: 546 MIWVYGNSFESFLVAVANPNQLALERWANENGIEGDFDSLCGDKRAKDYLLGELNKIAKE 605
            IWVYG+SF+S LVAV  PN   + RWA + G    F+ LC     K++++ EL   A++
Sbjct: 544 DIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKSTAEK 603

Query: 606 KKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMY 655
            KL+ FE+IKA+ ++  PFD+ERDL+T T K +R  LLKYYQ  ID MY
Sbjct: 604 NKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMY 652

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0A01.7e-30374.01Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9T0093.2e-29772.19Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Q9C7W48.9e-29270.45Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
M4ISH11.6e-22454.73Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Q9XIA95.5e-21753.83Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0KV210.0e+00100.00AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051550... [more]
A0A1S3BTT70.0e+0097.88long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103493094 PE... [more]
A0A5A7TP410.0e+0097.84Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5D3D1K30.0e+0097.53Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1J8030.0e+0093.03long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC11148340... [more]
Match NameE-valueIdentityDescription
XP_004149746.10.0e+00100.00long chain acyl-CoA synthetase 4 [Cucumis sativus] >KGN53403.1 hypothetical prot... [more]
XP_008451943.10.0e+0097.88PREDICTED: long chain acyl-CoA synthetase 4-like [Cucumis melo] >XP_008451944.1 ... [more]
KAA0044904.10.0e+0097.84long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa][more]
XP_038876793.10.0e+0095.15long chain acyl-CoA synthetase 4-like isoform X1 [Benincasa hispida][more]
TYK16566.10.0e+0097.53long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G23850.11.2e-30474.01AMP-dependent synthetase and ligase family protein [more]
AT4G11030.12.3e-29872.19AMP-dependent synthetase and ligase family protein [more]
AT1G64400.16.3e-29370.45AMP-dependent synthetase and ligase family protein [more]
AT1G49430.13.9e-21853.83long-chain acyl-CoA synthetase 2 [more]
AT2G47240.12.0e-19349.00AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 65..524
e-value: 6.7E-100
score: 334.6
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 43..516
e-value: 4.0E-88
score: 297.8
NoneNo IPR availablePANTHERPTHR43272:SF81LONG CHAIN ACYL-COA SYNTHETASE 4-LIKEcoord: 5..657
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 5..657
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 74..654
e-value: 0.0
score: 803.357
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 43..655
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 227..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G06350.1CSPI04G06350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity