CSPI04G06270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G06270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWD_REPEATS_REGION domain-containing protein
LocationChr4: 4323820 .. 4328485 (+)
RNA-Seq ExpressionCSPI04G06270
SyntenyCSPI04G06270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAAGAATGCGGCACTAAACCCTGAAGTAAGAAGGAGAAGTGCGATTTTTGTAGCAGGTGAAGGCGCGAACAAAGAATTGAGAGTTTCCACAATGGAGAAGGCTATGGCGACCTTTCACAAAGGCACTCTCTGTTCAGCTTGGAACTACTCCTCCACCAGATTAGCCACCGCTTCTTCCGACGGAACCCTCGTCATCTTTGATTCTCCCCACCCTTCTTCTTCCTCCACCTCCTTTACTCCCACCTCAAACTTCAAGGTTCTTTTACTTCCATTTCTCTCAATTTAACTTCATCATCTTACCTCAACTTTACTGTTGCTTACACATAATCTCACCTTTCTAAATGCCATGTGTTCTTAAGTCAAGAGAGAGGGGATATTAAATCATGTCTTTTAAGAGGACATGTTTCGTGTCCAGTGATATATTATGGGCTTTTTTTTCTTCTTCTTCTTCTGAATTTTCTTAAATTAATACGAAGATCATTCAATGAATTTATAACCTTGAAATCTTATCTATTTTCTTTCTTTTATTTTCCCCCCTGAGGGGGTGATCCATCTTTGTAGGCGCACGAGGTTGCAATTACCAAGATTGTTTGGGTACCTCCGGAGTATGGTGATTCTGTTGCGTGCATATGTTCAGATGGAACTTCATCATTGTGGGAGGAGGTTGTTGAAGGTGGTAACCGTTTGGTTTTTGAGGACCCTGGGTCCTCTCAAATTTTATTGTTGCATTAGTTTTGCCTATTTCTTTCATCCTATCTTCCCCTTCTCTTGGCCAATGAGTATGGTACATAGACCTTTATTTTACTGAAATTCTTCAAGTGTTCTAATCACCTCTGTAAATTCATTACCTTTTTCTGCTTTGTAGATGCCCAGCCCCTACAGTGGAAGCTATGCAAACTCTTCAAAAGTAATTCATCTCAAATCCTAGACTTGCAATTTGGAAATTCCTCCTCTGGTCTGAAAATGGTAAATTCTGTTGCTTCTTCTCATTCCCTTTCTGGATGTATATCCCAGAGTGCTGTTACAGATGAATGATGCCTGTGCTTTCTATTTGGCAAGCCCTATTGATTTTTAATGCATACAATTACTTCATTCTTGTTGACGTCAGAACATAACATATCATCATTTCTCCCTCCATGATTACAAGGATTTATTTGAATATAAATATGATAGTAGAATTAACAGTGATACACTTATGATGGTTCAAATATTTTCTTGTTGTTTCATTCTCTTTACATTTTTATTTTGCGTTCATGATAGCTTATTTATCATTAAAAATACCCTTTACATTTTCCCCTTTCAGATTGCTGCATTTTCAGATGGTTATATAAAAGTTTATGAGCTTATGGATCCTTTGAATTTGAAGAACTGGCAACTTCAGGTGATCAGTCTTCTAATATTTTTTCAAATTGTCTAATTTTATTTGGCTGCCAAAGTTATTGTTAGAATAAATTATTCCTGTGTGATTGGCCCTTGAGGACATCCAATAAAAATGGAACTTGTTCCTGTATGATCTTACAATCTGAAGAAATATATCTAGTAATCCTTTTTGTCAGACTTTCAATCAATAGATTTCTTCTTTAATAATAAAGGAAACTGAACTTTTGATTTCCTTTAATTAAAAACTATCTATGTTTTTAACCTACTGATGAATCGAACCACTTCACAGCTATATTCCAATGTTCTAATGCATCTCCCTTCTGTAAATTCCACAATGTATTACTGCATAATTAGATATGGATATTTGTGCACTTTTTTTGGTGAATTGTCCATTATTTGCTGTACTACTGTTATGTCAGAACCCTCATCAGTCAACAGTATTACCAACTATTCTTGGGGAATCACCTGAATTTTTATGTGAAAAACTTTTCAGTTTTCATTTTGTTGTTTTGAAGATTGTGTTTAGAGAATTTGGAGGTTTGGAATATTTTGTAAAGTCAAACTTGTCCTCGTTCTTCATCTTCCCTCCCTCGTCACTTTATGTCTCTTTATAAAAAGGAAAAAGAAGTGAGAGAAAAGTGTGGTGTGCATGTGCCCATGAACTCTTCTGAAGGAAGTATTTATTTTTGGTCCTTTACCTTCTCTGTGGCCTTTTTATTGACATTTGAAGTGTCTAAAAGAGAAACGGTCATGAATGGTTGGAAACTTTTATGGCATTCATCTAGAAGCCCAGATGGGCAAACATCTGCAGGGCGCTGAGTTTTAGGCTGAATTTCATTCCAAGTAAACAATGAGCTATTAAATATTTTGACTCCTATTCTCTACTTTGGTTCAGGCTGAATTTCAGAATGTTATTGATTCCATTTCTACAGTCAGGAAATCTTCTTGTTCATCTGCAAGTATATCATGGAATCCTCATCGAGGGGAGCAGCAATCAAGCTTTGTTCTTGCTTTTAGTTCCAATACACCGCAGCTTAATTCGGCCAAGGTATTCCTAGTTTTTCATATCTTATCACTGCAATCAAAATTGTGATGCATCTAGGTGTTCGCAGCTTGCTGGAAGTCTTCCATGATTGCATTAACCATAAAAATTGCACACTACAGTTAAATGGCATTTTTCCTGCACAGGTTTGGGAATTTGATCAGGCTCATCACAGATGGCTTCCTGTGGCAGAGTTGGCTTTGACTGCTGAGAAAGGTGATGAGGTTTATGCTGTCGCGTGGGCACAAAATATTGGAAGGTAATGAACTAATTTCTAAAGGAACTACCTATGTTAGGTGTATGTGTGGCGTGTTTATAACTTTTAGAACACACAAAATTCAGCAAATAATTATATCCCCATATAACTTTACACTTTCTTTTAACAAATCTAATAAACTATTTTGTTAAGTGCTAACTAATACTAGAACACTAGACAGAAAATTGCCTGGGGAATTTAAACGAGTTCCTTTAATTGGCAATATCAAGAAAATGAACTTCATTCAACGTCACAACTTTTTCTAAGAAAGCTTGAAAGGGAGACTTCAAAATGCAATTCTTTCCAAATTTCTTTTTTAATTCTTGATGATATACCCTGAAGATTTTGATTTTCTCCATTTGAAGCTCCTGGGAAATTCCTTTGATAGCATTTCTCCATAGGATGGATATGTGCTCAAAAGCCAAGGTGCTAACCAGGGGAAATGATATATTATGTTGAACTCTTGCTATAAAATGGCTAAGATTTATAGGAGCAGTGGAGGAATAGGTTGTTTGACTTCCCCCATTTTCTTTTCACAAAGACAATCCATACTTGGAAAGAGGTGATGTTTGGGAAGCATTCTATGCTTATACGAGTACATCAGACGACTTTTTCAACCAGCAGCAATTTCCAAAAGGAATATACGATCTTCAGAGATACAAAGAGCCAAATACATTTGACAATGTACTGACTTATACTATGCATTCTATTTCTTAAAGTTAGGAAAAATAGAACAGACAAATCTCCAAATTCTTTATCATATAAAGGTCCCATTTGACCCGAGTTCAGATGTAGGTCTCAACATCCATGGTATTGACATTACCCTTTCTTTCAAGAACTAAAAGAGTATCTGAACAGTAAATTTAGAGGCTTTTCCTCCTAGCCAACTCATAGTCTAGAGAGTGTTCCCTTGCAGTCCTTTAATTTCTTTACACGTCACAAACTTCCTGGTGCTATTCCTGATAACTGTTTGTAAGGATCACCATGACAAGTTTTGATATAAACACATTTATTTTACGTGCTAAAATCCTTCAAACATGTTGAATAAGTTTTAATAATAGTGATATTGTGTTGAAGTACCTTTTAGGCCATATGAGGTAATAGCTGTTGCAACCCAGAGAGGAATTGCAATATGGCATCTTGGATTAAGCCCCGACACGGATGGAAGGCTCTCAAGAGAAAGAGTTGCTCTGCTTTCTGGGCATAATGGCGAGGTATCTCTTAAAATTCTCTGTTCTCTTCTTCCTGTTTCCTTATATATATTTCTTGAAAATCTGAAGGCAGATGAAATCGAAACATCCATTGTTTAAGAGAAACACCTGGTGTAGAGAAGAGTTCATTTGAATCCATGTCATTGAAACCTTATAGAATAAATTCAAAACATTCGCAGTCATAGAAAGTTGGCTGCAAATGTTTCTAGAATTTTGTTGGTATGATTCCATGAACTGCTTTTTGAAGATTTTGTCTGTTTTGATTATCCTTAGACAATGAGGAAGAGGTGGTACGTAATTGATAATCATGTTTTCACTTGCACTTTCAGGTGTGGGAGATGGTGTGGGACATGGGTGGTATGACATTAGCATCAACAGGAAAGGACGGGATGGTACGGTTGTGGCAATCCAACCTCAATGGTGTTTGGCACCAACATGCTGCATTTGAGCCGACTTCTTAAAATTGAGAATGTTTTGTTTCTTTTTGATGTAACCACTTCCTGTATTCAATTTAAGGAAGAACTTCGAAATAATATTATTTGTCTTGTTGAGTGGGGGGCTAATGTCAGGGATACCTTATTGGAGACCCTTGGGGAAAATCAACTGTCATTGATGCTTTTATTTCTCCAAATTGCTTTTGCTGAGTTGAGACTTGTGTTGCTAAAGTATGTATTTCACGCTGACAAGAAGAAAGAATAAAACTGCACTTGAATTAGAGTTTGAGAATCCGAAGGAAAATGAGTATGAGTTTAAAACTAAGGACAATATAGTAT

mRNA sequence

AAAAGAAGAATGCGGCACTAAACCCTGAAGTAAGAAGGAGAAGTGCGATTTTTGTAGCAGGTGAAGGCGCGAACAAAGAATTGAGAGTTTCCACAATGGAGAAGGCTATGGCGACCTTTCACAAAGGCACTCTCTGTTCAGCTTGGAACTACTCCTCCACCAGATTAGCCACCGCTTCTTCCGACGGAACCCTCGTCATCTTTGATTCTCCCCACCCTTCTTCTTCCTCCACCTCCTTTACTCCCACCTCAAACTTCAAGGCGCACGAGGTTGCAATTACCAAGATTGTTTGGGTACCTCCGGAGTATGGTGATTCTGTTGCGTGCATATGTTCAGATGGAACTTCATCATTGTGGGAGGAGGTTGTTGAAGATGCCCAGCCCCTACAGTGGAAGCTATGCAAACTCTTCAAAAGTAATTCATCTCAAATCCTAGACTTGCAATTTGGAAATTCCTCCTCTGGTCTGAAAATGATTGCTGCATTTTCAGATGGTTATATAAAAGTTTATGAGCTTATGGATCCTTTGAATTTGAAGAACTGGCAACTTCAGGCTGAATTTCAGAATGTTATTGATTCCATTTCTACAGTCAGGAAATCTTCTTGTTCATCTGCAAGTATATCATGGAATCCTCATCGAGGGGAGCAGCAATCAAGCTTTGTTCTTGCTTTTAGTTCCAATACACCGCAGCTTAATTCGGCCAAGGTTTGGGAATTTGATCAGGCTCATCACAGATGGCTTCCTGTGGCAGAGTTGGCTTTGACTGCTGAGAAAGGTGATGAGGTTTATGCTGTCGCGTGGGCACAAAATATTGGAAGGCCATATGAGGTAATAGCTGTTGCAACCCAGAGAGGAATTGCAATATGGCATCTTGGATTAAGCCCCGACACGGATGGAAGGCTCTCAAGAGAAAGAGTTGCTCTGCTTTCTGGGCATAATGGCGAGGTGTGGGAGATGGTGTGGGACATGGGTGGTATGACATTAGCATCAACAGGAAAGGACGGGATGGTACGGTTGTGGCAATCCAACCTCAATGGTGTTTGGCACCAACATGCTGCATTTGAGCCGACTTCTTAAAATTGAGAATGTTTTGTTTCTTTTTGATGTAACCACTTCCTGTATTCAATTTAAGGAAGAACTTCGAAATAATATTATTTGTCTTGTTGAGTGGGGGGCTAATGTCAGGGATACCTTATTGGAGACCCTTGGGGAAAATCAACTGTCATTGATGCTTTTATTTCTCCAAATTGCTTTTGCTGAGTTGAGACTTGTGTTGCTAAAGTATGTATTTCACGCTGACAAGAAGAAAGAATAAAACTGCACTTGAATTAGAGTTTGAGAATCCGAAGGAAAATGAGTATGAGTTTAAAACTAAGGACAATATAGTAT

Coding sequence (CDS)

ATGGAGAAGGCTATGGCGACCTTTCACAAAGGCACTCTCTGTTCAGCTTGGAACTACTCCTCCACCAGATTAGCCACCGCTTCTTCCGACGGAACCCTCGTCATCTTTGATTCTCCCCACCCTTCTTCTTCCTCCACCTCCTTTACTCCCACCTCAAACTTCAAGGCGCACGAGGTTGCAATTACCAAGATTGTTTGGGTACCTCCGGAGTATGGTGATTCTGTTGCGTGCATATGTTCAGATGGAACTTCATCATTGTGGGAGGAGGTTGTTGAAGATGCCCAGCCCCTACAGTGGAAGCTATGCAAACTCTTCAAAAGTAATTCATCTCAAATCCTAGACTTGCAATTTGGAAATTCCTCCTCTGGTCTGAAAATGATTGCTGCATTTTCAGATGGTTATATAAAAGTTTATGAGCTTATGGATCCTTTGAATTTGAAGAACTGGCAACTTCAGGCTGAATTTCAGAATGTTATTGATTCCATTTCTACAGTCAGGAAATCTTCTTGTTCATCTGCAAGTATATCATGGAATCCTCATCGAGGGGAGCAGCAATCAAGCTTTGTTCTTGCTTTTAGTTCCAATACACCGCAGCTTAATTCGGCCAAGGTTTGGGAATTTGATCAGGCTCATCACAGATGGCTTCCTGTGGCAGAGTTGGCTTTGACTGCTGAGAAAGGTGATGAGGTTTATGCTGTCGCGTGGGCACAAAATATTGGAAGGCCATATGAGGTAATAGCTGTTGCAACCCAGAGAGGAATTGCAATATGGCATCTTGGATTAAGCCCCGACACGGATGGAAGGCTCTCAAGAGAAAGAGTTGCTCTGCTTTCTGGGCATAATGGCGAGGTGTGGGAGATGGTGTGGGACATGGGTGGTATGACATTAGCATCAACAGGAAAGGACGGGATGGTACGGTTGTGGCAATCCAACCTCAATGGTGTTTGGCACCAACATGCTGCATTTGAGCCGACTTCTTAA

Protein sequence

MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVAITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNSSSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPHRGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIGRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTGKDGMVRLWQSNLNGVWHQHAAFEPTS*
Homology
BLAST of CSPI04G06270 vs. ExPASy Swiss-Prot
Match: Q93VR9 (Protein SEH1 OS=Arabidopsis thaliana OX=3702 GN=SEH1 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-123
Identity = 207/325 (63.69%), Postives = 261/325 (80.31%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           M K+MAT   GT CS+WN S  RLA  S +G L I++S   +SSS++F+ TS  +  E +
Sbjct: 1   MAKSMATLDSGTTCSSWNQSGDRLAAGSLNGKLSIYES--STSSSSTFSCTSKVRVSESS 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVW+P EYGD+VAC+C DG+ S+WEE+ EDA  L+WKLCK  K+ SSQ+LD+QFG S
Sbjct: 61  IVKIVWLPSEYGDAVACVCEDGSLSIWEELSEDAHGLEWKLCKSMKNKSSQVLDVQFGVS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
              LKM+AA+SDGY++V+EL++PL LKNWQLQAEFQNVIDS+ST+ K S  SAS+SWNP 
Sbjct: 121 RKSLKMVAAYSDGYLRVFELLNPLELKNWQLQAEFQNVIDSLSTLGKPSSLSASVSWNPM 180

Query: 181 RGEQQS-SFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNI 240
           +GE+Q  SFVLAF+S++P LNS+K+WEFD+AH+RWL VAELAL  +KGD VYA++WA NI
Sbjct: 181 KGEEQEPSFVLAFNSDSPHLNSSKIWEFDEAHNRWLAVAELALPEDKGDPVYALSWAPNI 240

Query: 241 GRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLAST 300
           GRPYEV+AVAT +GI IWH+GL+PD +GRL  ++V+ LSGH GEVW+M WDM GMTLAST
Sbjct: 241 GRPYEVVAVATHKGIGIWHVGLAPDLEGRLPVKKVSSLSGHQGEVWQMEWDMSGMTLAST 300

Query: 301 GKDGMVRLWQSNLNGVWHQHAAFEP 325
           G DGMV+LWQSNLNG WH+ A  EP
Sbjct: 301 GSDGMVKLWQSNLNGEWHEQATLEP 323

BLAST of CSPI04G06270 vs. ExPASy Swiss-Prot
Match: C1BK83 (Nucleoporin SEH1 OS=Osmerus mordax OX=8014 GN=seh1l PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.4e-34
Identity = 94/327 (28.75%), Postives = 162/327 (49.54%), Query Frame = 0

Query: 3   KAMATFHKGTLCS-AWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVAI 62
           +++A  HK  +   ++++   R+AT SSD ++ ++D     S +  +  T+++K H  ++
Sbjct: 5   RSIAADHKDLIHDVSYDFHGRRMATCSSDQSVKVWD----KSDNGEWNCTASWKTHSGSV 64

Query: 63  TKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFK-----SNSSQILDLQ 122
            ++ W  PE+G  +A    D T+++WEE+V ++   Q  L    K      + + + D++
Sbjct: 65  WRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKQRGLSHWIKRTTLVDSRTSVTDVK 124

Query: 123 FGNSSSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASIS 182
           F     GL +    +DG +++YE  D +NL  W LQ E            K SCS   IS
Sbjct: 125 FAPKHMGLMLTTCSADGVVRIYEAPDVMNLSQWSLQHEIS---------CKLSCS--CIS 184

Query: 183 WNPHRGEQQSSFVLAFS--SNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVA 242
           WNP      S  +   S  SNT      +++E+ +   ++  V  L       D V+ +A
Sbjct: 185 WNPSSSRAHSPMIAVGSDDSNTAYSGKVQIYEYVENTRKYAKVETLMTVT---DPVHDIA 244

Query: 243 WAQNIGRPYEVIAVATQRGIAIWHL-----GLSPDTDGRLSRERVALLSGHNGEVWEMVW 302
           +A N+GR + V+A+AT + + I+ L       S     +L  +  A   GHN +VW + W
Sbjct: 245 FAPNLGRSFHVLAIAT-KDVRIFKLIPMRKESSSSGPTKLEVQLQAQFDGHNSQVWRVSW 304

Query: 303 DMGGMTLASTGKDGMVRLWQSNLNGVW 317
           ++    LAS+G DG VRLW++N    W
Sbjct: 305 NITSTLLASSGDDGCVRLWKANYMDNW 312

BLAST of CSPI04G06270 vs. ExPASy Swiss-Prot
Match: Q6GNF1 (Nucleoporin SEH1-B OS=Xenopus laevis OX=8355 GN=seh1l-b PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.4e-33
Identity = 88/327 (26.91%), Postives = 161/327 (49.24%), Query Frame = 0

Query: 3   KAMATFHKGTLCS-AWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVAI 62
           +++A  HK  +   ++++   R+AT SSD ++ ++D     S + ++  T+++K H  ++
Sbjct: 5   RSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWD----KSENVNWHCTASWKTHSGSV 64

Query: 63  TKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQ-----PLQWKLCKLFKSNSSQILDLQ 122
            ++ W  PE+G  +A    D T+++WEE+V ++         W        + + + D++
Sbjct: 65  WRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVK 124

Query: 123 FGNSSSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASIS 182
           F     GL +    +DG +++YE  D +NL  W LQ E            K SCS   IS
Sbjct: 125 FAPKHMGLMLATCSADGVVRIYEAPDVMNLSQWSLQHEIS---------CKLSCS--CIS 184

Query: 183 WNPHRGEQQSSFVLAFSSNTPQ--LNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVA 242
           WNP      S  +   S ++    +   +++E+++   ++   A+        D V+ +A
Sbjct: 185 WNPSSSRAHSPMIAVGSDDSSPNIMGKVQIYEYNENTRKY---AKAETLMSVSDPVHDIA 244

Query: 243 WAQNIGRPYEVIAVATQ--RGIAIWHLGLSPDTDG---RLSRERVALLSGHNGEVWEMVW 302
           +A N+GR + ++AVAT+  R   +  L     + G   +     VA    HN +VW + W
Sbjct: 245 FAPNLGRSFHILAVATKDVRIFTMKPLRKELSSSGGVTKFENHTVAQFDNHNSQVWRVSW 304

Query: 303 DMGGMTLASTGKDGMVRLWQSNLNGVW 317
           ++ G  LAS+G DG VRLW++N    W
Sbjct: 305 NITGTVLASSGDDGTVRLWKANYMDNW 313

BLAST of CSPI04G06270 vs. ExPASy Swiss-Prot
Match: Q4FZW5 (Nucleoporin SEH1-A OS=Xenopus laevis OX=8355 GN=seh1l-a PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 88/327 (26.91%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 3   KAMATFHKGTLCS-AWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVAI 62
           +++A  HK  +   ++++   R+AT SSD ++ ++D     S + ++  T+++K H  ++
Sbjct: 5   RSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWD----KSENGNWHCTASWKTHSGSV 64

Query: 63  TKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQ-----PLQWKLCKLFKSNSSQILDLQ 122
            ++ W  PE+G  +A    D T+++WEE+V ++         W        + + + D++
Sbjct: 65  WRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVK 124

Query: 123 FGNSSSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASIS 182
           F     GL +    +DG +++YE  D +NL  W LQ E            K SCS   IS
Sbjct: 125 FAPKHMGLMLATCSADGVVRIYEAPDVMNLSQWSLQHEIS---------CKLSCS--CIS 184

Query: 183 WNPHRGEQQSSFVLAFSSNTPQ--LNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVA 242
           WNP      S  +   S ++    +   +++E+++   ++   A+        D V+ +A
Sbjct: 185 WNPSSSRAHSPMIAVGSDDSSPNIMGKVQIYEYNENTRKY---AKAETLMSVSDPVHDIA 244

Query: 243 WAQNIGRPYEVIAVATQ--RGIAIWHLGLSPDTDGRLSR---ERVALLSGHNGEVWEMVW 302
           +A N+GR + ++AVAT+  R   +  L     + G +++     VA    HN +VW + W
Sbjct: 245 FAPNLGRSFHILAVATKDVRIFTMKPLRKELSSSGGVTKFEIHTVAQFDNHNSQVWRVSW 304

Query: 303 DMGGMTLASTGKDGMVRLWQSNLNGVW 317
           ++ G  LAS+G DG VRLW++N    W
Sbjct: 305 NITGTVLASSGDDGTVRLWKANYMDNW 313

BLAST of CSPI04G06270 vs. ExPASy Swiss-Prot
Match: Q96EE3 (Nucleoporin SEH1 OS=Homo sapiens OX=9606 GN=SEH1L PE=1 SV=3)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 94/327 (28.75%), Postives = 159/327 (48.62%), Query Frame = 0

Query: 3   KAMATFHKGTLCS-AWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVAI 62
           +++A  HK  +   ++++   R+AT SSD ++ ++D     S S  +  T+++K H  ++
Sbjct: 5   RSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWD----KSESGDWHCTASWKTHSGSV 64

Query: 63  TKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQ-----PLQWKLCKLFKSNSSQILDLQ 122
            ++ W  PE+G  +A    D T+++WEE+V ++         W        + + + D++
Sbjct: 65  WRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVK 124

Query: 123 FGNSSSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASIS 182
           F     GL +    +DG +++YE  D +NL  W LQ E            K SCS   IS
Sbjct: 125 FAPKHMGLMLATCSADGIVRIYEAPDVMNLSQWSLQHEIS---------CKLSCS--CIS 184

Query: 183 WNPHRGEQQSSFVLAFSSNTPQLNSAKVWEFD-QAHHRWLPVAELALTAEKGDEVYAVAW 242
           WNP      S  +   S ++     AKV  F+   + R    AE  +T    D V+ +A+
Sbjct: 185 WNPSSSRAHSPMIAVGSDDSSPNAMAKVQIFEYNENTRKYAKAETLMTVT--DPVHDIAF 244

Query: 243 AQNIGRPYEVIAVATQRGIAIWHLG------LSPDTDGRLSRERVALLSGHNGEVWEMVW 302
           A N+GR + ++A+AT + + I+ L        S     +     VA    HN +VW + W
Sbjct: 245 APNLGRSFHILAIAT-KDVRIFTLKPVRKELTSSGGPTKFEIHIVAQFDNHNSQVWRVSW 304

Query: 303 DMGGMTLASTGKDGMVRLWQSNLNGVW 317
           ++ G  LAS+G DG VRLW++N    W
Sbjct: 305 NITGTVLASSGDDGCVRLWKANYMDNW 313

BLAST of CSPI04G06270 vs. ExPASy TrEMBL
Match: A0A0A0KWZ8 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051460 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 323/326 (99.08%), Postives = 324/326 (99.39%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA
Sbjct: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFV+AFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVIAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIWHLGLSPD DGRLSRERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWHLGLSPDMDGRLSRERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 326

BLAST of CSPI04G06270 vs. ExPASy TrEMBL
Match: A0A1S3BTS7 (protein SEH1 OS=Cucumis melo OX=3656 GN=LOC103493086 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.8e-180
Identity = 313/326 (96.01%), Postives = 318/326 (97.55%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATF KGTLCSAWN+SS+RLATASSDGTLVIFDSPHPSSSSTS T TSNFKAHE A
Sbjct: 1   MEKAMATFDKGTLCSAWNHSSSRLATASSDGTLVIFDSPHPSSSSTSLTRTSNFKAHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPL+WKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLEWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPL+LK+WQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAH RWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHQRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIW LGLSPDTDGRLS ERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWRLGLSPDTDGRLSTERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 326

BLAST of CSPI04G06270 vs. ExPASy TrEMBL
Match: A0A6J1C5X6 (protein SEH1 OS=Momordica charantia OX=3673 GN=LOC111008268 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.8e-167
Identity = 292/327 (89.30%), Postives = 306/327 (93.58%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           ME AMA+F KGTLCSAWNYS++RLATAS DGTLVIFDSP PSSSSTS T TSNFKAHE A
Sbjct: 1   MESAMASFDKGTLCSAWNYSASRLATASVDGTLVIFDSPDPSSSSTSLTRTSNFKAHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCK FKSNS+Q+LD+QFGNS
Sbjct: 61  ILKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKRFKSNSAQMLDIQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFS  ++KVYELMDPL+LKNWQLQAEFQNVIDSISTVRK+SC SASISWNPH
Sbjct: 121 SSGLKMIAAFSHCHVKVYELMDPLDLKNWQLQAEFQNVIDSISTVRKASCLSASISWNPH 180

Query: 181 RGE-QQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNI 240
           RGE QQSSFVLA +SNTPQLNSAKVWEFDQAH RWLPVAELAL A+KGDEVYAVAWAQNI
Sbjct: 181 RGERQQSSFVLALNSNTPQLNSAKVWEFDQAHQRWLPVAELALPADKGDEVYAVAWAQNI 240

Query: 241 GRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLAST 300
           GRPYEVIAVATQ+GIAIWHLG +PD DGRLS ERVALLSGHNGEVWEM WDMGGMTLAST
Sbjct: 241 GRPYEVIAVATQKGIAIWHLGSNPDMDGRLSTERVALLSGHNGEVWEMEWDMGGMTLAST 300

Query: 301 GKDGMVRLWQSNLNGVWHQHAAFEPTS 327
           GKDGMVRLWQSNLNGVWHQ AAFEPTS
Sbjct: 301 GKDGMVRLWQSNLNGVWHQLAAFEPTS 327

BLAST of CSPI04G06270 vs. ExPASy TrEMBL
Match: A0A5A7TTR9 (Protein SEH1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003660 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.5e-165
Identity = 293/326 (89.88%), Postives = 298/326 (91.41%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATF KGTLCSAWN+SS+RLATASSDGTL                      AHE A
Sbjct: 1   MEKAMATFDKGTLCSAWNHSSSRLATASSDGTL----------------------AHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPL+WKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLEWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPL+LK+WQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAH RWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHQRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIW LGLSPDTDGRLS ERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWRLGLSPDTDGRLSTERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 304

BLAST of CSPI04G06270 vs. ExPASy TrEMBL
Match: A0A6J1FTY0 (protein SEH1 OS=Cucurbita moschata OX=3662 GN=LOC111447274 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.9e-163
Identity = 284/327 (86.85%), Postives = 306/327 (93.58%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATF KGT+CSAWNYS++RL T S+DGT+VIFDS  PSSSSTS T TSNFKAHE A
Sbjct: 1   MEKAMATFDKGTVCSAWNYSASRLVTGSADGTVVIFDSLDPSSSSTSLTRTSNFKAHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVWVPPEYGDS+ACICSDG+SSLWEEVVEDAQPLQWKL K FKSNSSQI+D+QFGNS
Sbjct: 61  IFKIVWVPPEYGDSIACICSDGSSSLWEEVVEDAQPLQWKLSKHFKSNSSQIIDIQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDG +KVYELMDPL+LK+WQLQAEFQNVIDSISTVRK++C SASISWNP+
Sbjct: 121 SSGLKMIAAFSDGNLKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKAACLSASISWNPN 180

Query: 181 RGE-QQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNI 240
           RGE QQSSFVLAF S+TPQL+SAKVWEFDQAH RWLPVAELAL A+KGD VYAVAWAQNI
Sbjct: 181 RGERQQSSFVLAFRSDTPQLDSAKVWEFDQAHQRWLPVAELALEADKGDGVYAVAWAQNI 240

Query: 241 GRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLAST 300
           GRPYEVIAVATQ+GIAIW LGL+PD+DGRLS ERVALLSGHNGEVW+M WDMGGMTLAST
Sbjct: 241 GRPYEVIAVATQKGIAIWRLGLNPDSDGRLSTERVALLSGHNGEVWDMEWDMGGMTLAST 300

Query: 301 GKDGMVRLWQSNLNGVWHQHAAFEPTS 327
           GKDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 GKDGMVRLWQSNLNGVWHQHAAFEPTS 327

BLAST of CSPI04G06270 vs. NCBI nr
Match: XP_004149752.1 (protein SEH1 [Cucumis sativus] >KGN53394.1 hypothetical protein Csa_015117 [Cucumis sativus])

HSP 1 Score: 665.6 bits (1716), Expect = 2.2e-187
Identity = 323/326 (99.08%), Postives = 324/326 (99.39%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA
Sbjct: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFV+AFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVIAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIWHLGLSPD DGRLSRERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWHLGLSPDMDGRLSRERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 326

BLAST of CSPI04G06270 vs. NCBI nr
Match: XP_008451932.1 (PREDICTED: protein SEH1 [Cucumis melo])

HSP 1 Score: 641.0 bits (1652), Expect = 5.7e-180
Identity = 313/326 (96.01%), Postives = 318/326 (97.55%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATF KGTLCSAWN+SS+RLATASSDGTLVIFDSPHPSSSSTS T TSNFKAHE A
Sbjct: 1   MEKAMATFDKGTLCSAWNHSSSRLATASSDGTLVIFDSPHPSSSSTSLTRTSNFKAHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPL+WKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLEWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPL+LK+WQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAH RWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHQRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIW LGLSPDTDGRLS ERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWRLGLSPDTDGRLSTERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 326

BLAST of CSPI04G06270 vs. NCBI nr
Match: XP_038897768.1 (protein SEH1 [Benincasa hispida])

HSP 1 Score: 612.1 bits (1577), Expect = 2.9e-171
Identity = 297/326 (91.10%), Postives = 307/326 (94.17%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMAT  KGTLCSAWNYS+ RLAT S+DGTL+IFDSP PSSSSTS T TSNFK HE A
Sbjct: 1   MEKAMATLGKGTLCSAWNYSANRLATGSADGTLLIFDSPDPSSSSTSLTRTSNFKVHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCK FK NSSQILD+QFGNS
Sbjct: 61  IIKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKHFKCNSSQILDIQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDG+IKVYELMDPL+LK+WQLQAEFQNVIDSISTVRK+ CSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGHIKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKAFCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQ SFVLAFSSNTPQLNSAKVWEFDQAH RWLPVAELALTA+KGDEVYAVAWAQNIG
Sbjct: 181 RGEQQPSFVLAFSSNTPQLNSAKVWEFDQAHQRWLPVAELALTADKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQ+GIAIW LGLSPDTDGRLS ERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQKGIAIWRLGLSPDTDGRLSTERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHA FEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHATFEPTS 326

BLAST of CSPI04G06270 vs. NCBI nr
Match: XP_022136602.1 (protein SEH1 [Momordica charantia])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 292/327 (89.30%), Postives = 306/327 (93.58%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           ME AMA+F KGTLCSAWNYS++RLATAS DGTLVIFDSP PSSSSTS T TSNFKAHE A
Sbjct: 1   MESAMASFDKGTLCSAWNYSASRLATASVDGTLVIFDSPDPSSSSTSLTRTSNFKAHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCK FKSNS+Q+LD+QFGNS
Sbjct: 61  ILKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKRFKSNSAQMLDIQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFS  ++KVYELMDPL+LKNWQLQAEFQNVIDSISTVRK+SC SASISWNPH
Sbjct: 121 SSGLKMIAAFSHCHVKVYELMDPLDLKNWQLQAEFQNVIDSISTVRKASCLSASISWNPH 180

Query: 181 RGE-QQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNI 240
           RGE QQSSFVLA +SNTPQLNSAKVWEFDQAH RWLPVAELAL A+KGDEVYAVAWAQNI
Sbjct: 181 RGERQQSSFVLALNSNTPQLNSAKVWEFDQAHQRWLPVAELALPADKGDEVYAVAWAQNI 240

Query: 241 GRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLAST 300
           GRPYEVIAVATQ+GIAIWHLG +PD DGRLS ERVALLSGHNGEVWEM WDMGGMTLAST
Sbjct: 241 GRPYEVIAVATQKGIAIWHLGSNPDMDGRLSTERVALLSGHNGEVWEMEWDMGGMTLAST 300

Query: 301 GKDGMVRLWQSNLNGVWHQHAAFEPTS 327
           GKDGMVRLWQSNLNGVWHQ AAFEPTS
Sbjct: 301 GKDGMVRLWQSNLNGVWHQLAAFEPTS 327

BLAST of CSPI04G06270 vs. NCBI nr
Match: KAA0044921.1 (protein SEH1 [Cucumis melo var. makuwa] >TYK16548.1 protein SEH1 [Cucumis melo var. makuwa])

HSP 1 Score: 592.0 bits (1525), Expect = 3.1e-165
Identity = 293/326 (89.88%), Postives = 298/326 (91.41%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           MEKAMATF KGTLCSAWN+SS+RLATASSDGTL                      AHE A
Sbjct: 1   MEKAMATFDKGTLCSAWNHSSSRLATASSDGTL----------------------AHEAA 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPL+WKLCKLFKSNSSQILDLQFGNS
Sbjct: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLEWKLCKLFKSNSSQILDLQFGNS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
           SSGLKMIAAFSDGYIKVYELMDPL+LK+WQLQAEFQNVIDSISTVRKSSCSSASISWNPH
Sbjct: 121 SSGLKMIAAFSDGYIKVYELMDPLDLKSWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180

Query: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNIG 240
           RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAH RWLPVAELALTAEKGDEVYAVAWAQNIG
Sbjct: 181 RGEQQSSFVLAFSSNTPQLNSAKVWEFDQAHQRWLPVAELALTAEKGDEVYAVAWAQNIG 240

Query: 241 RPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLASTG 300
           RPYEVIAVATQRGIAIW LGLSPDTDGRLS ERVALLSGHNGEVWEM WDMGGMTLASTG
Sbjct: 241 RPYEVIAVATQRGIAIWRLGLSPDTDGRLSTERVALLSGHNGEVWEMEWDMGGMTLASTG 300

Query: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 327
           KDGMVRLWQSNLNGVWHQHAAFEPTS
Sbjct: 301 KDGMVRLWQSNLNGVWHQHAAFEPTS 304

BLAST of CSPI04G06270 vs. TAIR 10
Match: AT1G64350.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.3e-124
Identity = 207/325 (63.69%), Postives = 261/325 (80.31%), Query Frame = 0

Query: 1   MEKAMATFHKGTLCSAWNYSSTRLATASSDGTLVIFDSPHPSSSSTSFTPTSNFKAHEVA 60
           M K+MAT   GT CS+WN S  RLA  S +G L I++S   +SSS++F+ TS  +  E +
Sbjct: 1   MAKSMATLDSGTTCSSWNQSGDRLAAGSLNGKLSIYES--STSSSSTFSCTSKVRVSESS 60

Query: 61  ITKIVWVPPEYGDSVACICSDGTSSLWEEVVEDAQPLQWKLCKLFKSNSSQILDLQFGNS 120
           I KIVW+P EYGD+VAC+C DG+ S+WEE+ EDA  L+WKLCK  K+ SSQ+LD+QFG S
Sbjct: 61  IVKIVWLPSEYGDAVACVCEDGSLSIWEELSEDAHGLEWKLCKSMKNKSSQVLDVQFGVS 120

Query: 121 SSGLKMIAAFSDGYIKVYELMDPLNLKNWQLQAEFQNVIDSISTVRKSSCSSASISWNPH 180
              LKM+AA+SDGY++V+EL++PL LKNWQLQAEFQNVIDS+ST+ K S  SAS+SWNP 
Sbjct: 121 RKSLKMVAAYSDGYLRVFELLNPLELKNWQLQAEFQNVIDSLSTLGKPSSLSASVSWNPM 180

Query: 181 RGEQQS-SFVLAFSSNTPQLNSAKVWEFDQAHHRWLPVAELALTAEKGDEVYAVAWAQNI 240
           +GE+Q  SFVLAF+S++P LNS+K+WEFD+AH+RWL VAELAL  +KGD VYA++WA NI
Sbjct: 181 KGEEQEPSFVLAFNSDSPHLNSSKIWEFDEAHNRWLAVAELALPEDKGDPVYALSWAPNI 240

Query: 241 GRPYEVIAVATQRGIAIWHLGLSPDTDGRLSRERVALLSGHNGEVWEMVWDMGGMTLAST 300
           GRPYEV+AVAT +GI IWH+GL+PD +GRL  ++V+ LSGH GEVW+M WDM GMTLAST
Sbjct: 241 GRPYEVVAVATHKGIGIWHVGLAPDLEGRLPVKKVSSLSGHQGEVWQMEWDMSGMTLAST 300

Query: 301 GKDGMVRLWQSNLNGVWHQHAAFEP 325
           G DGMV+LWQSNLNG WH+ A  EP
Sbjct: 301 GSDGMVKLWQSNLNGEWHEQATLEP 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VR91.8e-12363.69Protein SEH1 OS=Arabidopsis thaliana OX=3702 GN=SEH1 PE=1 SV=1[more]
C1BK833.4e-3428.75Nucleoporin SEH1 OS=Osmerus mordax OX=8014 GN=seh1l PE=2 SV=1[more]
Q6GNF16.4e-3326.91Nucleoporin SEH1-B OS=Xenopus laevis OX=8355 GN=seh1l-b PE=2 SV=1[more]
Q4FZW51.1e-3226.91Nucleoporin SEH1-A OS=Xenopus laevis OX=8355 GN=seh1l-a PE=2 SV=1[more]
Q96EE31.1e-3228.75Nucleoporin SEH1 OS=Homo sapiens OX=9606 GN=SEH1L PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0KWZ81.1e-18799.08WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A1S3BTS72.8e-18096.01protein SEH1 OS=Cucumis melo OX=3656 GN=LOC103493086 PE=3 SV=1[more]
A0A6J1C5X67.8e-16789.30protein SEH1 OS=Momordica charantia OX=3673 GN=LOC111008268 PE=3 SV=1[more]
A0A5A7TTR91.5e-16589.88Protein SEH1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003660 P... [more]
A0A6J1FTY06.9e-16386.85protein SEH1 OS=Cucurbita moschata OX=3662 GN=LOC111447274 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149752.12.2e-18799.08protein SEH1 [Cucumis sativus] >KGN53394.1 hypothetical protein Csa_015117 [Cucu... [more]
XP_008451932.15.7e-18096.01PREDICTED: protein SEH1 [Cucumis melo][more]
XP_038897768.12.9e-17191.10protein SEH1 [Benincasa hispida][more]
XP_022136602.11.6e-16689.30protein SEH1 [Momordica charantia][more]
KAA0044921.13.1e-16589.88protein SEH1 [Cucumis melo var. makuwa] >TYK16548.1 protein SEH1 [Cucumis melo v... [more]
Match NameE-valueIdentityDescription
AT1G64350.11.3e-12463.69Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 1..37
e-value: 0.22
score: 20.6
coord: 98..139
e-value: 2.3
score: 14.7
coord: 270..309
e-value: 1.1E-5
score: 34.9
coord: 47..88
e-value: 0.57
score: 18.6
IPR001680WD40 repeatPFAMPF00400WD40coord: 276..308
e-value: 0.0036
score: 18.1
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 277..309
score: 11.912015
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 9..323
e-value: 1.9E-49
score: 170.5
IPR037363Sec13/Seh1 familyPANTHERPTHR11024NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBERcoord: 1..325
IPR037597Nucleoporin Seh1PANTHERPTHR11024:SF3NUCLEOPORIN SEH1coord: 1..325
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 277..309
score: 9.941962
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 13..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G06270.1CSPI04G06270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034198 cellular response to amino acid starvation
biological_process GO:1904263 positive regulation of TORC1 signaling
biological_process GO:0015031 protein transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031080 nuclear pore outer ring
cellular_component GO:0035859 Seh1-associated complex
molecular_function GO:0005515 protein binding
molecular_function GO:0005198 structural molecule activity