CSPI04G04610 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04610
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEthylene-insensitive protein 3
LocationChr4: 2996114 .. 2997862 (-)
RNA-Seq ExpressionCSPI04G04610
SyntenyCSPI04G04610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAAAGAAATAAAACGATAAGTTAGTGATGTAATGAAATGGCATATGTGTTCAACATGCAAGCTCCCTACACCCATTCTTCAACCATCTCTTTCTCTCTCTTCTTCCTCTCTTTCAAAAACCTCCAAAGTCAAATTTACTCCTCCTTAATGGCAAACTTTGTCTCCAACCTTCAATCACCGCCATTCTCAATTTCTTCCCATTATATATATATACCCCTTCCCAACAATGGACCCAATTCACTGTATCTCAATTTCCCCCCCCCCAATTTTATAAACTACATATTACCTTCCTCATCTCACTTTAAATGATGGTGGAGTTCCATGGAGAAATCCGATCCCATAACGATGAAGACGACCGAGACCAAGAACCAGAAGAGGAAATTAGCTACGATGATCTCAAGAAACGAATGTGGAGAGATCGACAACGGATGAAGAAGATGAAGGAGAGGCATGATGAAGAAGAGCCTGAATCTGCTGCAAGAGAAGAAGCTTCTCGCCGTAAGAAAATGGCTAGAGCTCAAGATTCCATTCTTAAGTGCATGGATAAAATCATGGAAGCTTGTAAAGCTCAAGGGTTTGTTTATGGAATCGTTCCGGAAAAGGGAAAGCCGGTCACTGGCTCTTCCGAGAGCCTCCGTGAATGGTGGAAAGATGATGTCCGATTCGAACAAGATGCTCCAATGGCTATAGCGAAATTTCTCCCCAAAGTAATTGAAGAATCAGGGATTGATCCCAATTCGTTTTTGCATTTACTCACCGATTTACAGGACACAACATTGGGATCCATTCTCTCCGCTTTGATGCAGCACTGTATTCCTCCACAGAGGAAATTCCCACTGGAAAAGGGTTTAGCGCCGCCATGGTGGCCGACGGGTAACGAGCTTTGGTGGGGTGAACAGGGCGCCGCCGGTGGTCATGGTGTTCCGCCGTATAAAAAGCCCCACGATCTCAAAAAGGCGTGGAAAATCAGTGTCTTGGCGGCGGTGATTAAACACATGTCGCCTGATTTAGACAACATGAAGAAATTAATTAGGCAATCGAAGAATTTACAAGCGAAAATGACAGCGAAAGAGACAATCACCTGGGCTAAAGTTGTGAATCAAGAAGAAGCTCTGATGGACTTAACAAAAAAGAGCTTAAGAATCACCGACAAAGAAGAAGAACACAACAAAAACAGAGATAAACAGAGCAGCGATGAGATTATGACGAAACGAAAGTGTGTATTCGAACAAGAACCCATATTGGAATCGTTGTTATACCCTTGCCAGAATCAATGGTGTCCGCAGAGTGAAGCGGTAATGGGATTTATGGACAAGAAAGCAAGAACAGAGCATGAGACACAGTGCATTTGCGGAGGAGAGAGGTCAGAGGAATTTTCGGACGAGCAATCGATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGGGGAGGGCGGAGGCGGGGAGTGAGGAGGCGAGGATAGAAGATGCTGGAGATGGGTCGGGGAGCAGCACGGCGGAGGATTACGGGAGTGGGTACTGGAATATGGATTTGAATGCGAGTCCGGCGGAGGATTTGAGTGGGCAACAACAAGATTCTACTTCCATTTGGGATTTGAGATATGATTGGGGAGCTGAAGAATAATGAAGATCAACAATCACTCCCTTTTTATATCTCAATTTTGATGCTTCAAACGGAAAAACTAGTTTTGAAGAAGTTTTGAATTTTGAAATTTTAAATCTTTTCTTGG

mRNA sequence

AAAAAAGAAAGAAATAAAACGATAAGTTAGTGATGTAATGAAATGGCATATGTGTTCAACATGCAAGCTCCCTACACCCATTCTTCAACCATCTCTTTCTCTCTCTTCTTCCTCTCTTTCAAAAACCTCCAAAGTCAAATTTACTCCTCCTTAATGGCAAACTTTGTCTCCAACCTTCAATCACCGCCATTCTCAATTTCTTCCCATTATATATATATACCCCTTCCCAACAATGGACCCAATTCACTGTATCTCAATTTCCCCCCCCCCAATTTTATAAACTACATATTACCTTCCTCATCTCACTTTAAATGATGGTGGAGTTCCATGGAGAAATCCGATCCCATAACGATGAAGACGACCGAGACCAAGAACCAGAAGAGGAAATTAGCTACGATGATCTCAAGAAACGAATGTGGAGAGATCGACAACGGATGAAGAAGATGAAGGAGAGGCATGATGAAGAAGAGCCTGAATCTGCTGCAAGAGAAGAAGCTTCTCGCCGTAAGAAAATGGCTAGAGCTCAAGATTCCATTCTTAAGTGCATGGATAAAATCATGGAAGCTTGTAAAGCTCAAGGGTTTGTTTATGGAATCGTTCCGGAAAAGGGAAAGCCGGTCACTGGCTCTTCCGAGAGCCTCCGTGAATGGTGGAAAGATGATGTCCGATTCGAACAAGATGCTCCAATGGCTATAGCGAAATTTCTCCCCAAAGTAATTGAAGAATCAGGGATTGATCCCAATTCGTTTTTGCATTTACTCACCGATTTACAGGACACAACATTGGGATCCATTCTCTCCGCTTTGATGCAGCACTGTATTCCTCCACAGAGGAAATTCCCACTGGAAAAGGGTTTAGCGCCGCCATGGTGGCCGACGGGTAACGAGCTTTGGTGGGGTGAACAGGGCGCCGCCGGTGGTCATGGTGTTCCGCCGTATAAAAAGCCCCACGATCTCAAAAAGGCGTGGAAAATCAGTGTCTTGGCGGCGGTGATTAAACACATGTCGCCTGATTTAGACAACATGAAGAAATTAATTAGGCAATCGAAGAATTTACAAGCGAAAATGACAGCGAAAGAGACAATCACCTGGGCTAAAGTTGTGAATCAAGAAGAAGCTCTGATGGACTTAACAAAAAAGAGCTTAAGAATCACCGACAAAGAAGAAGAACACAACAAAAACAGAGATAAACAGAGCAGCGATGAGATTATGACGAAACGAAAGTGTGTATTCGAACAAGAACCCATATTGGAATCGTTGTTATACCCTTGCCAGAATCAATGGTGTCCGCAGAGTGAAGCGGTAATGGGATTTATGGACAAGAAAGCAAGAACAGAGCATGAGACACAGTGCATTTGCGGAGGAGAGAGGTCAGAGGAATTTTCGGACGAGCAATCGATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGGGGAGGGCGGAGGCGGGGAGTGAGGAGGCGAGGATAGAAGATGCTGGAGATGGGTCGGGGAGCAGCACGGCGGAGGATTACGGGAGTGGGTACTGGAATATGGATTTGAATGCGAGTCCGGCGGAGGATTTGAGTGGGCAACAACAAGATTCTACTTCCATTTGGGATTTGAGATATGATTGGGGAGCTGAAGAATAATGAAGATCAACAATCACTCCCTTTTTATATCTCAATTTTGATGCTTCAAACGGAAAAACTAGTTTTGAAGAAGTTTTGAATTTTGAAATTTTAAATCTTTTCTTGG

Coding sequence (CDS)

ATGATGGTGGAGTTCCATGGAGAAATCCGATCCCATAACGATGAAGACGACCGAGACCAAGAACCAGAAGAGGAAATTAGCTACGATGATCTCAAGAAACGAATGTGGAGAGATCGACAACGGATGAAGAAGATGAAGGAGAGGCATGATGAAGAAGAGCCTGAATCTGCTGCAAGAGAAGAAGCTTCTCGCCGTAAGAAAATGGCTAGAGCTCAAGATTCCATTCTTAAGTGCATGGATAAAATCATGGAAGCTTGTAAAGCTCAAGGGTTTGTTTATGGAATCGTTCCGGAAAAGGGAAAGCCGGTCACTGGCTCTTCCGAGAGCCTCCGTGAATGGTGGAAAGATGATGTCCGATTCGAACAAGATGCTCCAATGGCTATAGCGAAATTTCTCCCCAAAGTAATTGAAGAATCAGGGATTGATCCCAATTCGTTTTTGCATTTACTCACCGATTTACAGGACACAACATTGGGATCCATTCTCTCCGCTTTGATGCAGCACTGTATTCCTCCACAGAGGAAATTCCCACTGGAAAAGGGTTTAGCGCCGCCATGGTGGCCGACGGGTAACGAGCTTTGGTGGGGTGAACAGGGCGCCGCCGGTGGTCATGGTGTTCCGCCGTATAAAAAGCCCCACGATCTCAAAAAGGCGTGGAAAATCAGTGTCTTGGCGGCGGTGATTAAACACATGTCGCCTGATTTAGACAACATGAAGAAATTAATTAGGCAATCGAAGAATTTACAAGCGAAAATGACAGCGAAAGAGACAATCACCTGGGCTAAAGTTGTGAATCAAGAAGAAGCTCTGATGGACTTAACAAAAAAGAGCTTAAGAATCACCGACAAAGAAGAAGAACACAACAAAAACAGAGATAAACAGAGCAGCGATGAGATTATGACGAAACGAAAGTGTGTATTCGAACAAGAACCCATATTGGAATCGTTGTTATACCCTTGCCAGAATCAATGGTGTCCGCAGAGTGAAGCGGTAATGGGATTTATGGACAAGAAAGCAAGAACAGAGCATGAGACACAGTGCATTTGCGGAGGAGAGAGGTCAGAGGAATTTTCGGACGAGCAATCGATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGGGGAGGGCGGAGGCGGGGAGTGAGGAGGCGAGGATAGAAGATGCTGGAGATGGGTCGGGGAGCAGCACGGCGGAGGATTACGGGAGTGGGTACTGGAATATGGATTTGAATGCGAGTCCGGCGGAGGATTTGAGTGGGCAACAACAAGATTCTACTTCCATTTGGGATTTGAGATATGATTGGGGAGCTGAAGAATAA

Protein sequence

MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAREEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRFEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEEFSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNASPAEDLSGQQQDSTSIWDLRYDWGAEE*
Homology
BLAST of CSPI04G04610 vs. ExPASy Swiss-Prot
Match: Q9LX16 (Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Arabidopsis thaliana OX=3702 GN=EIL4 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.9e-104
Identity = 199/352 (56.53%), Postives = 260/352 (73.86%), Query Frame = 0

Query: 21  EPEEEISYDDLKKRMWRDRQRMK---KMKERHDEEEPE-SAAREEASRRKKMARAQDSIL 80
           + EEEISYDDLK+RMW+DR  M+   K ++RH  +    +  R EASRRKKMAR+QDS+L
Sbjct: 15  DEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVL 74

Query: 81  KCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRFEQDAPMAIAKFL---- 140
           K M KIME CKA+GFVYGIVPEKGKP+TGSS+SLR WWK++V+F+Q+AP AI  +L    
Sbjct: 75  KYMMKIMEVCKAKGFVYGIVPEKGKPITGSSDSLRRWWKENVQFDQNAPDAITDYLALAA 134

Query: 141 ----PKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEKGLAPPWWP 200
                ++I++S    +S LH+L +LQDTTLGS+LSALMQHC+PPQR+FPLEKG+APPWWP
Sbjct: 135 AAAAAELIDKSS-SSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWP 194

Query: 201 TGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKKLIRQSKNL 260
           TG ELWWGEQGAA  HG PPY+KPHDL+K+WK+SVLAAVIKHMSP+L  +++L RQSK+L
Sbjct: 195 TGTELWWGEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSL 254

Query: 261 QAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIMTKRKCVFE 320
           Q KM AKET TW++V+NQEEAL+++  K L+I++ +++   +  K+ S+ +         
Sbjct: 255 QDKMMAKETDTWSRVLNQEEALLNI--KDLKISEDQDDQESSGSKRKSESM--------- 314

Query: 321 QEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEEFSDE 361
            EP     +Y CQN  CP+S+   GF DK +RT HE QC+ G  +    S E
Sbjct: 315 -EP--SKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGE 351

BLAST of CSPI04G04610 vs. ExPASy Swiss-Prot
Match: Q9FJQ5 (ETHYLENE INSENSITIVE 3-like 5 protein OS=Arabidopsis thaliana OX=3702 GN=EIL5 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 4.5e-98
Identity = 226/531 (42.56%), Postives = 299/531 (56.31%), Query Frame = 0

Query: 14  DEDDRDQEPE------EEISYDDLKKRMWRDRQRMKKMKERHDE------EEPESAA--- 73
           DEDD +++ +      EEISYDDLKKRMW+DR  M K+K++  +        P S+    
Sbjct: 17  DEDDLEEDVDEFERFGEEISYDDLKKRMWKDRNLMCKLKQQKRDNLNSVISSPSSSTSAS 76

Query: 74  ----------REEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSE 133
                     R EASRRKKMAR+QDS+LK M KIME CKAQGFVYGIVPEKGKPVTGSS+
Sbjct: 77  SSSSSSVIVRRTEASRRKKMARSQDSVLKYMMKIMEVCKAQGFVYGIVPEKGKPVTGSSD 136

Query: 134 SLREWWKDDVRFEQDAPMAIAKFL-----PKVIEESGIDPNSFLHLLTDLQDTTLGSILS 193
           SLR WWK++V+F+Q AP A++ +L       +     +DPNS++H+L +LQDTTLGS+LS
Sbjct: 137 SLRRWWKENVQFDQTAPNAVSDYLTLAAAQLISSNESLDPNSYIHMLHELQDTTLGSLLS 196

Query: 194 ALMQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAG-GHGVPPYKKPHDLKKAWKIS 253
           ALMQHC+PPQR+FPLEKGLAPPWWP G ELWWGEQGAA   HG PPY+KPHDL+KAWK+S
Sbjct: 197 ALMQHCVPPQRRFPLEKGLAPPWWPNGTELWWGEQGAAAFEHGPPPYRKPHDLRKAWKVS 256

Query: 254 VLAAVIKHMSPDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKS----- 313
           VLAAVIKHMSP+L+ +++L RQSK LQ KM AKET TW++V+NQEEA ++  K S     
Sbjct: 257 VLAAVIKHMSPNLERVRRLARQSKCLQDKMMAKETDTWSRVLNQEEARLNRLKISDDEDE 316

Query: 314 --------------------------------------------------LRITDKEEEH 373
                                                             L   D+E   
Sbjct: 317 DRDQEQARFTCFDQEPSLNTCFIVGQDQEPLGSMRKDKRVDQEFSSNDCFLVAQDQEPRK 376

Query: 374 NKNRDKQSS---------DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKK 433
            K  D++ S         + +  KRK  F ++  + S +Y CQN  CP S+  +GF+DK 
Sbjct: 377 GKKADQEWSPNSCFLVDQEPLGNKRKGEFVEKEAMLSNVYTCQNSSCPSSDVSLGFVDKN 436

Query: 434 ARTEHETQCICGGERSEEFSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSG 436
            RT HE +C+ G       S     D  ++S+    + +   A + +E+ R     DG+ 
Sbjct: 437 LRTGHEIECLYGTPELVNQSSGGGSDGFVRSIT--TSDDDYSASSKAEDTRDYHNQDGNW 496

BLAST of CSPI04G04610 vs. ExPASy Swiss-Prot
Match: Q9SLH0 (ETHYLENE INSENSITIVE 3-like 1 protein OS=Arabidopsis thaliana OX=3702 GN=EIL1 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 7.5e-85
Identity = 178/391 (45.52%), Postives = 237/391 (60.61%), Query Frame = 0

Query: 23  EEEISYDDLKKRMWRDRQRMKKMKERHDE-----EEPESAAREEASRRKKMARAQDSILK 82
           ++E+  D+L+KRMWRD+ R+K++KE+  +     +  +    +E +RRKKM+RAQD ILK
Sbjct: 43  DDEMDVDELEKRMWRDKMRLKRLKEQQSKCKEGVDGSKQRQSQEQARRKKMSRAQDGILK 102

Query: 83  CMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRFEQDAPMAIAKFLPK-VI 142
            M K+ME CKAQGFVYGI+PEKGKPVTG+S++LREWWKD VRF+++ P AIAK+  +  I
Sbjct: 103 YMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAIAKYQSENNI 162

Query: 143 EESGIDPNSFL----HLLTDLQDTTLGSILSALMQHCIPPQRKFPLEKGLAPPWWPTGNE 202
                D NS +    H L +LQDTTLGS+LSALMQHC PPQR+FPLEKG++PPWWP GNE
Sbjct: 163 SGGSNDCNSLVGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVSPPWWPNGNE 222

Query: 203 LWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKKLIRQSKNLQAKM 262
            WW + G     G PPYKKPHDLKKAWK+ VL AVIKHMSPD+  ++KL+RQSK LQ KM
Sbjct: 223 EWWPQLGLPNEQGPPPYKKPHDLKKAWKVGVLTAVIKHMSPDIAKIRKLVRQSKCLQDKM 282

Query: 263 TAKETITWAKVVNQEEAL---------------MDLTKKSLRITD---------KEEEHN 322
           TAKE+ TW  ++NQEE +                 L   SL I D         ++E+H 
Sbjct: 283 TAKESATWLAIINQEEVVARELYPESCPPLSSSSSLGSGSLLINDCSEYDVEGFEKEQHG 342

Query: 323 KNRDKQSSDEIMTKRKCVF-----EQEPILESLL-------------------------- 348
            + +++  + +M      F     +  PI E +                           
Sbjct: 343 FDVEERKPEIVMMHPLASFGVAKMQHFPIKEEVATTVNLEFTRKRKQNNDMNVMVMDRSA 402

BLAST of CSPI04G04610 vs. ExPASy Swiss-Prot
Match: O24606 (Protein ETHYLENE INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=EIN3 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 4.1e-83
Identity = 178/404 (44.06%), Postives = 238/404 (58.91%), Query Frame = 0

Query: 3   VEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR--- 62
           V+F    ++  D    D   ++EI  D+L++RMWRD+ R+K++KE+   +E   AA+   
Sbjct: 23  VDFCPVPQAEPDSIVEDDYTDDEIDVDELERRMWRDKMRLKRLKEQDKGKEGVDAAKQRQ 82

Query: 63  -EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDV 122
            +E +RRKKM+RAQD ILK M K+ME CKAQGFVYGI+PE GKPVTG+S++LREWWKD V
Sbjct: 83  SQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPENGKPVTGASDNLREWWKDKV 142

Query: 123 RFEQDAPMAIAKF-----LPKVIE-ESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPP 182
           RF+++ P AI K+     +P + E  + I P    H L +LQDTTLGS+LSALMQHC PP
Sbjct: 143 RFDRNGPAAITKYQAENNIPGIHEGNNPIGPTP--HTLQELQDTTLGSLLSALMQHCDPP 202

Query: 183 QRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMS 242
           QR+FPLEKG+ PPWWP G E WW + G     G  PYKKPHDLKKAWK+ VL AVIKHM 
Sbjct: 203 QRRFPLEKGVPPPWWPNGKEDWWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMF 262

Query: 243 PDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALM--------------------- 302
           PD+  ++KL+RQSK LQ KMTAKE+ TW  ++NQEE+L                      
Sbjct: 263 PDIAKIRKLVRQSKCLQDKMTAKESATWLAIINQEESLARELYPESCPPLSLSGGSCSLL 322

Query: 303 --DLTKKSLRITDKEEEHN--------------------------KNRDKQSSDEIMTKR 348
             D ++  +   +KE  +                           K      + E M KR
Sbjct: 323 MNDCSQYDVEGFEKESHYEVEELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGNSEFMRKR 382

BLAST of CSPI04G04610 vs. ExPASy Swiss-Prot
Match: Q8W3L9 (Protein ETHYLENE-INSENSITIVE 3-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=EIL2 PE=2 SV=2)

HSP 1 Score: 298.1 bits (762), Expect = 1.6e-79
Identity = 183/415 (44.10%), Postives = 239/415 (57.59%), Query Frame = 0

Query: 5   FHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKE-RHDEEEPESAA----- 64
           F  E  S  D+DD D +  ++I  ++L++RMWRDR R K++KE +      ES A     
Sbjct: 59  FAAEDESDGDDDDDDDDDVDDI--EELERRMWRDRVRHKRLKELQQSRAGRESRAGDAGG 118

Query: 65  -------REEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLR 124
                   +E +RRKKM+RAQD ILK M K+ME C AQGFVYGI+PEKGKPV+G+S++LR
Sbjct: 119 GGRQQRQSQEQARRKKMSRAQDGILKYMLKMMEVCNAQGFVYGIIPEKGKPVSGASDNLR 178

Query: 125 EWWKDDVRFEQDAPMAIAKF-----LPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALM 184
            WWK+ VRF+++ P AIAK+     +P    ++G    +  H L +LQDTTLGS+LSALM
Sbjct: 179 SWWKEKVRFDRNGPAAIAKYQADNAVPGCDGDAGGAAPAGPHSLHELQDTTLGSLLSALM 238

Query: 185 QHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAA 244
           QHC PPQR+FPLEKG+ PPWWP G+E WW E G     G PPYKKPHDLKKAWK++VL A
Sbjct: 239 QHCDPPQRRFPLEKGVPPPWWPEGSEAWWPEAGVPKELGPPPYKKPHDLKKAWKVAVLTA 298

Query: 245 VIKHMSPDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALM----------DLTKK 304
           VIKHMSPD+D +++L+RQSK LQ KMTAKE +TW  V+ QEE L            L+  
Sbjct: 299 VIKHMSPDVDKVRRLVRQSKCLQDKMTAKEIVTWLAVLKQEEDLYLKLHPGALPPPLSAA 358

Query: 305 SLRIT----------DKEEEHNKNRDKQSSDEI----------------------MTKRK 348
           S   +          D +E  N N  K  +D                        + K +
Sbjct: 359 SFNASVSGEYDVEGVDGDEAGNNNLQKAQNDATAFMDLTTTMDAALSNNKFLIMPLMKEE 418

BLAST of CSPI04G04610 vs. ExPASy TrEMBL
Match: A0A5A7SXT3 (Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold494G00050 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.5e-242
Identity = 420/447 (93.96%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MMVEFHGEIRSH-NDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR 60
           MMVEFHGEIRSH NDEDDRDQEPEEEISYDDLKKRMWRDRQR+KKMKERHDEEEPESAAR
Sbjct: 1   MMVEFHGEIRSHNNDEDDRDQEPEEEISYDDLKKRMWRDRQRLKKMKERHDEEEPESAAR 60

Query: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120
           EEASRRKKMARAQDSILKCMDK MEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR
Sbjct: 61  EEASRRKKMARAQDSILKCMDKNMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120

Query: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180
           FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALM HCIPPQRKFPLE
Sbjct: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMHHCIPPQRKFPLE 180

Query: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMK 240
           KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHM PDLDNM+
Sbjct: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMLPDLDNMR 240

Query: 241 KLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITD---KEEEHNKNRDKQSS 300
           KLIRQSKNLQAKMTAKET+TWAKVVNQEEAL+ LTKKSLRITD   +EEEHNKNRDKQ S
Sbjct: 241 KLIRQSKNLQAKMTAKETLTWAKVVNQEEALLKLTKKSLRITDEEEEEEEHNKNRDKQCS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCVFEQEP+ +S+LYPCQNQWCPQSE +MGF+DK+ARTEHETQCIC GERSEE
Sbjct: 301 DEIMTKRKCVFEQEPVSDSMLYPCQNQWCPQSEVLMGFIDKRARTEHETQCICRGERSEE 360

Query: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420
           FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSST EDYGSGYWNMDLNA
Sbjct: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTVEDYGSGYWNMDLNA 420

Query: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           SP EDLSGQQQD+TSIWDLRY+WGAEE
Sbjct: 421 SPGEDLSGQQQDATSIWDLRYEWGAEE 447

BLAST of CSPI04G04610 vs. ExPASy TrEMBL
Match: A0A5D3CY70 (Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005870 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 2.9e-241
Identity = 421/447 (94.18%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MMVEFHGEIRSH-NDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR 60
           MMVEFHGEIRSH NDEDDRDQEPEEEISYDDLKKRMWRDRQR+KKMKERHDEEEPESAAR
Sbjct: 1   MMVEFHGEIRSHNNDEDDRDQEPEEEISYDDLKKRMWRDRQRLKKMKERHDEEEPESAAR 60

Query: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120
           EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR
Sbjct: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120

Query: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180
           FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE
Sbjct: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180

Query: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMK 240
           KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHM PDLDNM+
Sbjct: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMLPDLDNMR 240

Query: 241 KLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITD---KEEEHNKNRDKQSS 300
           KLIRQSKNLQAKMTAKET+TWAKVVNQEEAL+ LTKKSLRITD   +EEEHNKNRDKQ S
Sbjct: 241 KLIRQSKNLQAKMTAKETLTWAKVVNQEEALLKLTKKSLRITDEEEEEEEHNKNRDKQCS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCV EQEPI +S+LYPCQNQWCPQSE +MGF+DK+ARTEHETQCIC GERSEE
Sbjct: 301 DEIMTKRKCVSEQEPISDSMLYPCQNQWCPQSEVLMGFIDKRARTEHETQCICRGERSEE 360

Query: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420
           FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSST EDYGSGYWNMDLNA
Sbjct: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTVEDYGSGYWNMDLNA 420

Query: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           SP EDLSGQQQD+TSI DLRY+WGAEE
Sbjct: 421 SPGEDLSGQQQDATSILDLRYEWGAEE 447

BLAST of CSPI04G04610 vs. ExPASy TrEMBL
Match: A0A6J1J695 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita maxima OX=3661 GN=LOC111482971 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 6.2e-191
Identity = 344/462 (74.46%), Postives = 391/462 (84.63%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIR   D D++D+  EEE+SYDDLKKRMW+DRQRMK++KE+ D E+PESAARE
Sbjct: 1   MMVEFHGEIRCSGDGDEQDK--EEELSYDDLKKRMWKDRQRMKQLKEKWDTEKPESAARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILK MDKIM+ACKA+GFVYGIVPEKGKPVTGSSESLREWW+DDVRF
Sbjct: 61  EASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAP+AIA+FLPKV+EE+G+D  SFLH+L DLQDTTLGSILSAL+QHCIPPQRKFPLEK
Sbjct: 121 EQDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNM+K
Sbjct: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIM 300
           L+RQSKNLQAKMTAKET+TWAKVVNQEE+L+ LTKKSL+IT  E+EH +NR++Q +  I+
Sbjct: 241 LVRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSDEDEHGENREEQYAGGII 300

Query: 301 TKRKCVFEQ--EPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEEFS 360
           +KRKC+FEQ      +S LY CQNQ CPQSEA MGF DKKARTE+ETQCI         +
Sbjct: 301 SKRKCIFEQVAAAAADSALYACQNQRCPQSEATMGFTDKKARTENETQCI------YREA 360

Query: 361 DEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYW-------- 420
           ++QSIDTHLKSVVEWMNWEL RAE  S+E R EDA DGSGSST E+YGS YW        
Sbjct: 361 EDQSIDTHLKSVVEWMNWELSRAEPSSDEGRSEDAVDGSGSSTVENYGSSYWKGGVGEAS 420

Query: 421 ----------NMDLNASPAEDLSGQQQDSTSIWDLRYDWGAE 443
                     +MDLNASPA+ L G Q+D+TS+WDL Y+W  +
Sbjct: 421 VDEAFEMHRESMDLNASPAKVLGGGQEDATSVWDLIYEWAED 454

BLAST of CSPI04G04610 vs. ExPASy TrEMBL
Match: A0A6J1EN74 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita moschata OX=3662 GN=LOC111436029 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.1e-190
Identity = 345/462 (74.68%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIRS  D D++D+  EEE+SYDDLKKRMW+DRQRMK++KE+ D EEPES ARE
Sbjct: 1   MMVEFHGEIRSSGDGDEQDK--EEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILK MDKIM+ACKA+GFVYGIVPEKGKPVTGSSESLREWW+DDVRF
Sbjct: 61  EASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAP+AIA+FLPKV+EE+G+D  SFLH+L DLQDTTLGSILSAL+QHCIPPQRKFPLEK
Sbjct: 121 EQDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNM+K
Sbjct: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIM 300
           L+RQSKNLQAKMTAKET+TWAKVVNQEE+L+ LTKKSL+IT  E+ H++N ++Q +  I+
Sbjct: 241 LVRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGII 300

Query: 301 TKRKCVFEQ--EPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEEFS 360
           TKRK +FEQ      +S LY CQNQ CPQSEA MGF DK ARTEHETQCI         +
Sbjct: 301 TKRKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCI------YREA 360

Query: 361 DEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYW-------- 420
           ++++IDTHLKSVVEWMNWEL RAE  SEE R EDA DGSGSST E+YGS YW        
Sbjct: 361 EDENIDTHLKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAA 420

Query: 421 ----------NMDLNASPAEDLSGQQQDSTSIWDLRYDWGAE 443
                     +MDLNASPAEDL G QQD+TS+WDL Y+W  +
Sbjct: 421 VDEAFEMHRASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 454

BLAST of CSPI04G04610 vs. ExPASy TrEMBL
Match: A0A6J1C3X1 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Momordica charantia OX=3673 GN=LOC111008154 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.1e-184
Identity = 339/477 (71.07%), Postives = 385/477 (80.71%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIR H D D+ +QE EEEISYDDLKKRMW+DRQR+KKMKE  D EEP+SAARE
Sbjct: 1   MMVEFHGEIRPH-DGDNEEQEEEEEISYDDLKKRMWKDRQRLKKMKETRDSEEPDSAARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILK MDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWW+DDVRF
Sbjct: 61  EASRRKKMARAQDSILKYMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWRDDVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAP+AIA++LPKVIEE+G+D  SFLHLL DLQDTTLGSILSALMQHC+PPQRKFPLEK
Sbjct: 121 EQDAPIAIAEYLPKVIEETGLDTASFLHLLHDLQDTTLGSILSALMQHCMPPQRKFPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTG E+WWGEQGAAGGHGVPPYKKPHDLKKAWKIS+LAAVIKHMSPD+DNM+K
Sbjct: 181 GLAPPWWPTGKEIWWGEQGAAGGHGVPPYKKPHDLKKAWKISLLAAVIKHMSPDMDNMRK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIM 300
           L+RQSKNLQAKMTAKET+TW KVVNQEEAL++LTKKSL+IT +E E N+  ++++  E +
Sbjct: 241 LVRQSKNLQAKMTAKETLTWTKVVNQEEALLELTKKSLKITGEEHEENRAEEEETRAEEL 300

Query: 301 T-------------KRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQC 360
                         KRKC+F +E   ++ LY CQN+WCPQSE++MGF+DK ARTEHE QC
Sbjct: 301 VGGGGGTTHFRGNEKRKCLFNRETAGDTALYTCQNKWCPQSESLMGFVDKNARTEHEAQC 360

Query: 361 ICGGE---RSEEFSDEQSIDTHLKSVVEWMNWELGRAEA-GSEEARIEDAGDGSGSSTAE 420
           +        + +FSDE +ID HLKSVVEWMN EL RAE   SEEAR+ED GDGS S   E
Sbjct: 361 VYRNNAVVEAGQFSDEHAIDNHLKSVVEWMNLELARAEPNSSEEARMEDNGDGSESRAVE 420

Query: 421 DYGSGYW------------------NMDLNASPAEDLSGQQQDSTSIWDLRYDWGAE 443
           +YG+ YW                  +MDLN SP ED  G  QD+TSIWDLRY+W  E
Sbjct: 421 NYGTRYWKAGVGEATVDEAFEMHSESMDLNVSPTED-EGCHQDATSIWDLRYEWTEE 475

BLAST of CSPI04G04610 vs. NCBI nr
Match: XP_004146925.2 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sativus] >XP_031745658.1 putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sativus] >XP_031745659.1 putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sativus] >KAE8637151.1 hypothetical protein CSA_004633 [Cucumis sativus] >KAE8637169.1 hypothetical protein CSA_018800 [Cucumis sativus] >KAE8649152.1 hypothetical protein Csa_014592 [Cucumis sativus])

HSP 1 Score: 882.5 bits (2279), Expect = 1.5e-252
Identity = 440/447 (98.43%), Postives = 442/447 (98.88%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE
Sbjct: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF
Sbjct: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK
Sbjct: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK
Sbjct: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRIT----DKEEEHNKNRDKQSS 300
           LIRQSKNLQAKMTAKETITWAKVVNQEEALM+LTKKSLRIT    DKEEEHNKNRDKQSS
Sbjct: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMNLTKKSLRITDEEEDKEEEHNKNRDKQSS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE
Sbjct: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360

Query: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420
           FSDEQS+DTHLKSVVEWMNWELGRAEAG EEARIEDAGDGSGSSTAEDYGSGYWNMDLNA
Sbjct: 361 FSDEQSMDTHLKSVVEWMNWELGRAEAGREEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420

Query: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           SPAEDLSGQQQDSTSIWDLRYDWGAEE
Sbjct: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 447

BLAST of CSPI04G04610 vs. NCBI nr
Match: KAA0036012.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis melo var. makuwa])

HSP 1 Score: 847.0 bits (2187), Expect = 7.2e-242
Identity = 420/447 (93.96%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MMVEFHGEIRSH-NDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR 60
           MMVEFHGEIRSH NDEDDRDQEPEEEISYDDLKKRMWRDRQR+KKMKERHDEEEPESAAR
Sbjct: 1   MMVEFHGEIRSHNNDEDDRDQEPEEEISYDDLKKRMWRDRQRLKKMKERHDEEEPESAAR 60

Query: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120
           EEASRRKKMARAQDSILKCMDK MEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR
Sbjct: 61  EEASRRKKMARAQDSILKCMDKNMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120

Query: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180
           FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALM HCIPPQRKFPLE
Sbjct: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMHHCIPPQRKFPLE 180

Query: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMK 240
           KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHM PDLDNM+
Sbjct: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMLPDLDNMR 240

Query: 241 KLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITD---KEEEHNKNRDKQSS 300
           KLIRQSKNLQAKMTAKET+TWAKVVNQEEAL+ LTKKSLRITD   +EEEHNKNRDKQ S
Sbjct: 241 KLIRQSKNLQAKMTAKETLTWAKVVNQEEALLKLTKKSLRITDEEEEEEEHNKNRDKQCS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCVFEQEP+ +S+LYPCQNQWCPQSE +MGF+DK+ARTEHETQCIC GERSEE
Sbjct: 301 DEIMTKRKCVFEQEPVSDSMLYPCQNQWCPQSEVLMGFIDKRARTEHETQCICRGERSEE 360

Query: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420
           FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSST EDYGSGYWNMDLNA
Sbjct: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTVEDYGSGYWNMDLNA 420

Query: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           SP EDLSGQQQD+TSIWDLRY+WGAEE
Sbjct: 421 SPGEDLSGQQQDATSIWDLRYEWGAEE 447

BLAST of CSPI04G04610 vs. NCBI nr
Match: TYK16747.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis melo var. makuwa])

HSP 1 Score: 844.0 bits (2179), Expect = 6.1e-241
Identity = 421/447 (94.18%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MMVEFHGEIRSH-NDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR 60
           MMVEFHGEIRSH NDEDDRDQEPEEEISYDDLKKRMWRDRQR+KKMKERHDEEEPESAAR
Sbjct: 1   MMVEFHGEIRSHNNDEDDRDQEPEEEISYDDLKKRMWRDRQRLKKMKERHDEEEPESAAR 60

Query: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120
           EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR
Sbjct: 61  EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVR 120

Query: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180
           FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE
Sbjct: 121 FEQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLE 180

Query: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMK 240
           KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHM PDLDNM+
Sbjct: 181 KGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMLPDLDNMR 240

Query: 241 KLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITD---KEEEHNKNRDKQSS 300
           KLIRQSKNLQAKMTAKET+TWAKVVNQEEAL+ LTKKSLRITD   +EEEHNKNRDKQ S
Sbjct: 241 KLIRQSKNLQAKMTAKETLTWAKVVNQEEALLKLTKKSLRITDEEEEEEEHNKNRDKQCS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCV EQEPI +S+LYPCQNQWCPQSE +MGF+DK+ARTEHETQCIC GERSEE
Sbjct: 301 DEIMTKRKCVSEQEPISDSMLYPCQNQWCPQSEVLMGFIDKRARTEHETQCICRGERSEE 360

Query: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWNMDLNA 420
           FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSST EDYGSGYWNMDLNA
Sbjct: 361 FSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTVEDYGSGYWNMDLNA 420

Query: 421 SPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           SP EDLSGQQQD+TSI DLRY+WGAEE
Sbjct: 421 SPGEDLSGQQQDATSILDLRYEWGAEE 447

BLAST of CSPI04G04610 vs. NCBI nr
Match: XP_038892572.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Benincasa hispida])

HSP 1 Score: 732.3 bits (1889), Expect = 2.6e-207
Identity = 372/451 (82.48%), Postives = 403/451 (89.36%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIR  ND++D   E EEEISYDDLKKRMWRDRQRMKKMKE+ D EEPESAARE
Sbjct: 1   MMVEFHGEIRCPNDDED---EQEEEISYDDLKKRMWRDRQRMKKMKEKRDNEEPESAARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILK MDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWW+D+VRF
Sbjct: 61  EASRRKKMARAQDSILKYMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWRDEVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAPMAIAKFLPKVIEESG+DP+S LHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK
Sbjct: 121 EQDAPMAIAKFLPKVIEESGLDPHSVLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTG ELWWGEQG+AGGHGVPPYKKPHDLKKAWKISVLAAVIKHMS DLDNM+K
Sbjct: 181 GLAPPWWPTGKELWWGEQGSAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSHDLDNMRK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRIT--DKEEEHNKNRDKQSSDE 300
           L+RQSKNLQAKMTAKET+TW KVVNQEEAL+ LTK +L+IT  ++EEE  ++ D QS+  
Sbjct: 241 LVRQSKNLQAKMTAKETLTWGKVVNQEEALLQLTKNNLKITSDEEEEEDGEDSDAQSNGG 300

Query: 301 IMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCIC-GGERSEEF 360
           IMTKRKC FEQ    +S LYPCQNQWCPQS+A+MGF+DK AR+EHETQC+   GE +EEF
Sbjct: 301 IMTKRKCTFEQVTTSDSTLYPCQNQWCPQSKALMGFVDKNARSEHETQCVYRAGEGAEEF 360

Query: 361 SDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSGSSTAEDYGSGYWN-----M 420
           SD+QSID HLKSVVEWMNWEL RA  GS+E RIEDA DGSGSST EDYGSGYWN     +
Sbjct: 361 SDDQSIDIHLKSVVEWMNWELSRAAPGSDEVRIEDARDGSGSSTVEDYGSGYWNGGVGEV 420

Query: 421 DLNASPAEDLSGQQQDSTSIWDLRYDWGAEE 444
           DLNASPAEDL G QQD+TSIWDLRY+WGAEE
Sbjct: 421 DLNASPAEDLGG-QQDATSIWDLRYEWGAEE 447

BLAST of CSPI04G04610 vs. NCBI nr
Match: XP_031745709.1 (LOW QUALITY PROTEIN: putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sativus])

HSP 1 Score: 685.6 bits (1768), Expect = 2.8e-193
Identity = 345/367 (94.01%), Postives = 349/367 (95.10%), Query Frame = 0

Query: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60
           MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE
Sbjct: 1   MMVEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 60

Query: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120
           EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF
Sbjct: 61  EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRF 120

Query: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEK 180
           EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTT GSILSALMQHCIPPQRK PLEK
Sbjct: 121 EQDAPMAIAKFLPKVIEESGIDPNSFLHLLTDLQDTTXGSILSALMQHCIPPQRKXPLEK 180

Query: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240
           GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK
Sbjct: 181 GLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKK 240

Query: 241 LIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSL----RITDKEEEHNKNRDKQSS 300
           LIRQSKNLQAKMTAKETITWAKVVNQE +  +L KK L    R   +    NKNRDKQSS
Sbjct: 241 LIRQSKNLQAKMTAKETITWAKVVNQERSSDELNKKELKNHRRRRRQRRRTNKNRDKQSS 300

Query: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360
           DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE
Sbjct: 301 DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEE 360

Query: 361 FSDEQSI 364
           FSDEQS+
Sbjct: 361 FSDEQSM 367

BLAST of CSPI04G04610 vs. TAIR 10
Match: AT5G10120.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-105
Identity = 199/352 (56.53%), Postives = 260/352 (73.86%), Query Frame = 0

Query: 21  EPEEEISYDDLKKRMWRDRQRMK---KMKERHDEEEPE-SAAREEASRRKKMARAQDSIL 80
           + EEEISYDDLK+RMW+DR  M+   K ++RH  +    +  R EASRRKKMAR+QDS+L
Sbjct: 15  DEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVL 74

Query: 81  KCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRFEQDAPMAIAKFL---- 140
           K M KIME CKA+GFVYGIVPEKGKP+TGSS+SLR WWK++V+F+Q+AP AI  +L    
Sbjct: 75  KYMMKIMEVCKAKGFVYGIVPEKGKPITGSSDSLRRWWKENVQFDQNAPDAITDYLALAA 134

Query: 141 ----PKVIEESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPPQRKFPLEKGLAPPWWP 200
                ++I++S    +S LH+L +LQDTTLGS+LSALMQHC+PPQR+FPLEKG+APPWWP
Sbjct: 135 AAAAAELIDKSS-SSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWP 194

Query: 201 TGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKKLIRQSKNL 260
           TG ELWWGEQGAA  HG PPY+KPHDL+K+WK+SVLAAVIKHMSP+L  +++L RQSK+L
Sbjct: 195 TGTELWWGEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSL 254

Query: 261 QAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRDKQSSDEIMTKRKCVFE 320
           Q KM AKET TW++V+NQEEAL+++  K L+I++ +++   +  K+ S+ +         
Sbjct: 255 QDKMMAKETDTWSRVLNQEEALLNI--KDLKISEDQDDQESSGSKRKSESM--------- 314

Query: 321 QEPILESLLYPCQNQWCPQSEAVMGFMDKKARTEHETQCICGGERSEEFSDE 361
            EP     +Y CQN  CP+S+   GF DK +RT HE QC+ G  +    S E
Sbjct: 315 -EP--SKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGE 351

BLAST of CSPI04G04610 vs. TAIR 10
Match: AT5G65100.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-99
Identity = 226/531 (42.56%), Postives = 299/531 (56.31%), Query Frame = 0

Query: 14  DEDDRDQEPE------EEISYDDLKKRMWRDRQRMKKMKERHDE------EEPESAA--- 73
           DEDD +++ +      EEISYDDLKKRMW+DR  M K+K++  +        P S+    
Sbjct: 17  DEDDLEEDVDEFERFGEEISYDDLKKRMWKDRNLMCKLKQQKRDNLNSVISSPSSSTSAS 76

Query: 74  ----------REEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSE 133
                     R EASRRKKMAR+QDS+LK M KIME CKAQGFVYGIVPEKGKPVTGSS+
Sbjct: 77  SSSSSSVIVRRTEASRRKKMARSQDSVLKYMMKIMEVCKAQGFVYGIVPEKGKPVTGSSD 136

Query: 134 SLREWWKDDVRFEQDAPMAIAKFL-----PKVIEESGIDPNSFLHLLTDLQDTTLGSILS 193
           SLR WWK++V+F+Q AP A++ +L       +     +DPNS++H+L +LQDTTLGS+LS
Sbjct: 137 SLRRWWKENVQFDQTAPNAVSDYLTLAAAQLISSNESLDPNSYIHMLHELQDTTLGSLLS 196

Query: 194 ALMQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAG-GHGVPPYKKPHDLKKAWKIS 253
           ALMQHC+PPQR+FPLEKGLAPPWWP G ELWWGEQGAA   HG PPY+KPHDL+KAWK+S
Sbjct: 197 ALMQHCVPPQRRFPLEKGLAPPWWPNGTELWWGEQGAAAFEHGPPPYRKPHDLRKAWKVS 256

Query: 254 VLAAVIKHMSPDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKS----- 313
           VLAAVIKHMSP+L+ +++L RQSK LQ KM AKET TW++V+NQEEA ++  K S     
Sbjct: 257 VLAAVIKHMSPNLERVRRLARQSKCLQDKMMAKETDTWSRVLNQEEARLNRLKISDDEDE 316

Query: 314 --------------------------------------------------LRITDKEEEH 373
                                                             L   D+E   
Sbjct: 317 DRDQEQARFTCFDQEPSLNTCFIVGQDQEPLGSMRKDKRVDQEFSSNDCFLVAQDQEPRK 376

Query: 374 NKNRDKQSS---------DEIMTKRKCVFEQEPILESLLYPCQNQWCPQSEAVMGFMDKK 433
            K  D++ S         + +  KRK  F ++  + S +Y CQN  CP S+  +GF+DK 
Sbjct: 377 GKKADQEWSPNSCFLVDQEPLGNKRKGEFVEKEAMLSNVYTCQNSSCPSSDVSLGFVDKN 436

Query: 434 ARTEHETQCICGGERSEEFSDEQSIDTHLKSVVEWMNWELGRAEAGSEEARIEDAGDGSG 436
            RT HE +C+ G       S     D  ++S+    + +   A + +E+ R     DG+ 
Sbjct: 437 LRTGHEIECLYGTPELVNQSSGGGSDGFVRSIT--TSDDDYSASSKAEDTRDYHNQDGNW 496

BLAST of CSPI04G04610 vs. TAIR 10
Match: AT2G27050.1 (ETHYLENE-INSENSITIVE3-like 1 )

HSP 1 Score: 315.8 bits (808), Expect = 5.3e-86
Identity = 178/391 (45.52%), Postives = 237/391 (60.61%), Query Frame = 0

Query: 23  EEEISYDDLKKRMWRDRQRMKKMKERHDE-----EEPESAAREEASRRKKMARAQDSILK 82
           ++E+  D+L+KRMWRD+ R+K++KE+  +     +  +    +E +RRKKM+RAQD ILK
Sbjct: 43  DDEMDVDELEKRMWRDKMRLKRLKEQQSKCKEGVDGSKQRQSQEQARRKKMSRAQDGILK 102

Query: 83  CMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDVRFEQDAPMAIAKFLPK-VI 142
            M K+ME CKAQGFVYGI+PEKGKPVTG+S++LREWWKD VRF+++ P AIAK+  +  I
Sbjct: 103 YMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAIAKYQSENNI 162

Query: 143 EESGIDPNSFL----HLLTDLQDTTLGSILSALMQHCIPPQRKFPLEKGLAPPWWPTGNE 202
                D NS +    H L +LQDTTLGS+LSALMQHC PPQR+FPLEKG++PPWWP GNE
Sbjct: 163 SGGSNDCNSLVGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVSPPWWPNGNE 222

Query: 203 LWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMKKLIRQSKNLQAKM 262
            WW + G     G PPYKKPHDLKKAWK+ VL AVIKHMSPD+  ++KL+RQSK LQ KM
Sbjct: 223 EWWPQLGLPNEQGPPPYKKPHDLKKAWKVGVLTAVIKHMSPDIAKIRKLVRQSKCLQDKM 282

Query: 263 TAKETITWAKVVNQEEAL---------------MDLTKKSLRITD---------KEEEHN 322
           TAKE+ TW  ++NQEE +                 L   SL I D         ++E+H 
Sbjct: 283 TAKESATWLAIINQEEVVARELYPESCPPLSSSSSLGSGSLLINDCSEYDVEGFEKEQHG 342

Query: 323 KNRDKQSSDEIMTKRKCVF-----EQEPILESLL-------------------------- 348
            + +++  + +M      F     +  PI E +                           
Sbjct: 343 FDVEERKPEIVMMHPLASFGVAKMQHFPIKEEVATTVNLEFTRKRKQNNDMNVMVMDRSA 402

BLAST of CSPI04G04610 vs. TAIR 10
Match: AT3G20770.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 310.1 bits (793), Expect = 2.9e-84
Identity = 178/404 (44.06%), Postives = 238/404 (58.91%), Query Frame = 0

Query: 3   VEFHGEIRSHNDEDDRDQEPEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAAR--- 62
           V+F    ++  D    D   ++EI  D+L++RMWRD+ R+K++KE+   +E   AA+   
Sbjct: 23  VDFCPVPQAEPDSIVEDDYTDDEIDVDELERRMWRDKMRLKRLKEQDKGKEGVDAAKQRQ 82

Query: 63  -EEASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWKDDV 122
            +E +RRKKM+RAQD ILK M K+ME CKAQGFVYGI+PE GKPVTG+S++LREWWKD V
Sbjct: 83  SQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPENGKPVTGASDNLREWWKDKV 142

Query: 123 RFEQDAPMAIAKF-----LPKVIE-ESGIDPNSFLHLLTDLQDTTLGSILSALMQHCIPP 182
           RF+++ P AI K+     +P + E  + I P    H L +LQDTTLGS+LSALMQHC PP
Sbjct: 143 RFDRNGPAAITKYQAENNIPGIHEGNNPIGPTP--HTLQELQDTTLGSLLSALMQHCDPP 202

Query: 183 QRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMS 242
           QR+FPLEKG+ PPWWP G E WW + G     G  PYKKPHDLKKAWK+ VL AVIKHM 
Sbjct: 203 QRRFPLEKGVPPPWWPNGKEDWWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMF 262

Query: 243 PDLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALM--------------------- 302
           PD+  ++KL+RQSK LQ KMTAKE+ TW  ++NQEE+L                      
Sbjct: 263 PDIAKIRKLVRQSKCLQDKMTAKESATWLAIINQEESLARELYPESCPPLSLSGGSCSLL 322

Query: 303 --DLTKKSLRITDKEEEHN--------------------------KNRDKQSSDEIMTKR 348
             D ++  +   +KE  +                           K      + E M KR
Sbjct: 323 MNDCSQYDVEGFEKESHYEVEELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGNSEFMRKR 382

BLAST of CSPI04G04610 vs. TAIR 10
Match: AT1G73730.1 (ETHYLENE-INSENSITIVE3-like 3 )

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-74
Identity = 149/299 (49.83%), Postives = 200/299 (66.89%), Query Frame = 0

Query: 8   EIRSHNDEDDRDQE-------PEEEISYDDLKKRMWRDRQRMKKMKERHDEEEPESAARE 67
           +IR  N+ DD   +        +EEI  DDL++RMW+DR R+K++KER       +  +E
Sbjct: 10  DIRMENEPDDLASDNVAEIDVSDEEIDADDLERRMWKDRVRLKRIKERQKAGSQGAQTKE 69

Query: 68  ------EASRRKKMARAQDSILKCMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWW 127
                 + ++RKKM+RAQD ILK M K+ME CK +GFVYGI+PEKGKPV+GSS+++R WW
Sbjct: 70  TPKKISDQAQRKKMSRAQDGILKYMLKLMEVCKVRGFVYGIIPEKGKPVSGSSDNIRAWW 129

Query: 128 KDDVRFEQDAPMAIAKFLPKVIEESGIDPN-SFLHLLTDLQDTTLGSILSALMQHCIPPQ 187
           K+ V+F+++ P AIAK+  + +     D N +   +L DLQD TLGS+LS+LMQHC PPQ
Sbjct: 130 KEKVKFDKNGPAAIAKYEEECLAFGKSDGNRNSQFVLQDLQDATLGSLLSSLMQHCDPPQ 189

Query: 188 RKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSP 247
           RK+PLEKG  PPWWPTGNE WW + G       PPY+KPHDLKK WK+ VL AVI HM P
Sbjct: 190 RKYPLEKGTPPPWWPTGNEEWWVKLGLPKSQS-PPYRKPHDLKKMWKVGVLTAVINHMLP 249

Query: 248 DLDNMKKLIRQSKNLQAKMTAKETITWAKVVNQEEALMDLTKKSLRITDKEEEHNKNRD 293
           D+  +K+ +RQSK LQ KMTAKE+  W  V+NQEE+L+         ++  E H +  +
Sbjct: 250 DIAKIKRHVRQSKCLQDKMTAKESAIWLAVLNQEESLIQQPSSDNGNSNVTETHRRGNN 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LX161.9e-10456.53Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FJQ54.5e-9842.56ETHYLENE INSENSITIVE 3-like 5 protein OS=Arabidopsis thaliana OX=3702 GN=EIL5 PE... [more]
Q9SLH07.5e-8545.52ETHYLENE INSENSITIVE 3-like 1 protein OS=Arabidopsis thaliana OX=3702 GN=EIL1 PE... [more]
O246064.1e-8344.06Protein ETHYLENE INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=EIN3 PE=1 SV=1[more]
Q8W3L91.6e-7944.10Protein ETHYLENE-INSENSITIVE 3-like 2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A5A7SXT33.5e-24293.96Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucumis melo var. makuwa OX=11... [more]
A0A5D3CY702.9e-24194.18Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1J6956.2e-19174.46putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EN741.1e-19074.68putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1C3X11.1e-18471.07putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
XP_004146925.21.5e-25298.43putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sativus] >XP_031745658.1... [more]
KAA0036012.17.2e-24293.96putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis melo var. makuwa][more]
TYK16747.16.1e-24194.18putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis melo var. makuwa][more]
XP_038892572.12.6e-20782.48putative ETHYLENE INSENSITIVE 3-like 4 protein [Benincasa hispida][more]
XP_031745709.12.8e-19394.01LOW QUALITY PROTEIN: putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucumis sat... [more]
Match NameE-valueIdentityDescription
AT5G10120.11.4e-10556.53Ethylene insensitive 3 family protein [more]
AT5G65100.13.2e-9942.56Ethylene insensitive 3 family protein [more]
AT2G27050.15.3e-8645.52ETHYLENE-INSENSITIVE3-like 1 [more]
AT3G20770.12.9e-8444.06Ethylene insensitive 3 family protein [more]
AT1G73730.11.2e-7449.83ETHYLENE-INSENSITIVE3-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 28..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..429
NoneNo IPR availablePANTHERPTHR33305:SF29ETHYLENE INSENSITIVE 3-LIKE 5 PROTEINcoord: 14..438
IPR006957Ethylene insensitive 3PFAMPF04873EIN3coord: 29..268
e-value: 1.6E-109
score: 365.1
IPR006957Ethylene insensitive 3PANTHERPTHR33305ETHYLENE INSENSITIVE 3-LIKE 2 PROTEINcoord: 14..438
IPR023278Ethylene insensitive 3-like protein, DNA-binding domain superfamilyGENE3D1.10.3180.10coord: 140..277
e-value: 4.9E-64
score: 216.1
coord: 57..138
e-value: 4.3E-7
score: 31.5
IPR023278Ethylene insensitive 3-like protein, DNA-binding domain superfamilySUPERFAMILY116768DNA-binding domain of EIN3-likecoord: 147..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04610.1CSPI04G04610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009873 ethylene-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity