CSPI04G04540 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04540
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionlanC-like protein GCL1
LocationChr4: 2946982 .. 2952829 (+)
RNA-Seq ExpressionCSPI04G04540
SyntenyCSPI04G04540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCATTGTTCAATAACACCATCGATTACAATAAAGAGAGCTCCCCACGTTGTTTCTTTGCTTTTCGAAAATTATAATTATAATTTGTTTATTTTCTTTCTTTCTTTTAGAAAAACTAAAACAACAAACGTCATTCGCGTGTGTCTACGGAAATAATAATTCAATTTATTATTCGTAAAAGCGCGTGTTTCACACTTGCAATCCCGACCCACCGTTTATGCTCTCCCCAACCGCCATTGATTTTCCCTTTTAACACCATCACTCCAAAACACAACTCACTACCCTCTCCCTCCCTCTCCAGATTTCATTTCAATTCCTTCTTCTTACACTAATGTCTTCCCTCCTCCACCTCGCTTCATCTCAGGAAGATCGAGAAGAAGGTGGTGGAGGAGGAGGAATGATCCAGATTATTCCACCCAACACTATTCTTCTTCCTTCTCAAACCTTTCTCTCTGCAGCCGTCTCTTTCAAGAATCAGGTTTGTTTTACCTTTTTTTTCTTTTCTCGAGCTCCCAAGCGTTCATGGAAACGGTGTTGATTACACTGGTTTTTTTTTCTCTCTGCTGTTGAAATTTTGTTAGGTTGTGGAGCTTACTTGGAAACAGAGAGGTAACGCGGATATTGGAGTTGATCCGACGGTGTATAACGGCTTACTTGGGACGGCGGTAACTTGCCTGAGATCGTACGAGGCCACCGGAAACCACCAGGACTTGGTATTGTGTTCTGATATTGTAGATAAATGTACGGTTCTAGCACGTGCCTCTCACAGGTGATCATGTGACTTTTGCTTTCCCTTTCTCTATCCAATGATATGATTTATTTGGTTTGTTGCCTTTCCAATCATAAAATTTAAAACGTGAAGTTCATAAAATAGACGTCCAAAATTCAAGTTTTTGTTTCCAGTTTTTCGTTTTACCTAAATTTAGTTTTTCCTTTGATAATCTAAAATGAATTTCATAGGAAATGGTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAATTCAAAGTGATTTTAAATCCATTTTTACTTTTATAAAAATCTGATTATAATAGGATTTTGCAGTGTTTTATAGGATTTGGATAATATTACTTAGTTAACTTTCTCAATTCTATACAGCCCTTCCATAAAATTGTTAGATCTACTATCGACCATTGAACTTTTTGATTTTTTTAGTATAAGGGTTAGTTTTTGTGGTACAATTTGGCTCCAATTTATTATGGAGTTGTTCTTGCATTTTTACACCTGGGCCAACCATGAATTGTCATGTGTCAATGCATTATCTTTTAGTTTTCCCATCAAGCGATGACACCTCTTGGCTAGCAATGAAGAGTGAGTTTTTCCGATAAAGTAAAATCATCTTTCTTTAAAATTCTCTCCGTTTGTACTAACTAATAAAATTGTTCCAAAAAAAATATATAAAGTAATTAAAAAAAGAAAACTCAGATAGCAACGGAATATGTGATTAGATAATTTTTTTTTAATATTTCCACAAAGCTTCTGGACCCAGTTAGACCTGTTTGTGATTAAAATAATAAAAATCATCATATAATAAAATAGAGCAAAAAAACGAGTGCCACAAATATTGAGTTTTCAGAAAATTTTCAAAAGAGACAAACTTCTTTTGTCATATAAAAAATATCTCATGACCAGGCCTCTGGAATTATTTTTCCCCTTGATTTTAAATTATCTGCTCCTCAAACCCACTTTGAAAAGCAGAAAGTGTACTACTAAAAAGTAGTCTTCTTTTTTCTCATGATTTCTTTCTGTTTTTGGCGAAATTTTGTTGAATGATTAATGAGTGAAATAAAATCGTATTTTGAGACATGATATTATATAAGGTGGGGCAGTTCTTATGAATTTTGTTTTCATAATCTTCATTTCTTAAAAAATATAATGATGAAATTTATAGTATTAACCTCCCGGTTAAGTTCTTAGTTTAGTTCGTAAGCTTTCAAAAGTATTTAGTAGGTATTTGAAGCATAATAAGTCTTTTTGACTTTAAATTTGTTACTAATATGTTCTTAAAATTTTAATTTTGTATTTATACATCTTTACTTTATTCAAATTTTAAAAAATAATTAAATTGATAATTGGTCTATTATATGTACCTTTGGATTTTGTTTCTAATGGAACCTTAAAGTTTCACATTGCAGAGGTATAAGGACTTAAAAATACAAAATCAATAAAATTGTACGCGATAATTTTTCATGAAGATATTACCTTCCTGACATCTGAGAGATGATGGGTAATTAGTAACTGTTTTGCTAAAAGAAATAAATAAAGAAAAGAAAATAGGTATTGGAAGTATGGGATAGAAGTTAATGCTTATTTATTATTGTTGTTGTTTCCTTTTTAAAAGGAGAAATTATTGGTTTTAGGCATATGTTCTTTTTAGTTAATATATCACTTGGAAAGAATGTGGACCATATAGTTCTTGGAAGAGTTTGATGTGAAATAATTGAATATTTTGTTACTTTGGTATGGGCAGGCACTTGACTTTTCTTTGTGGTAGAGGAGGAGTTTATGCTCTTGGCGCCGTGGTATCTCATTACATGAGGGACCCTCAAAAGCTTGACTTCTTCTTCAATCTTTTTCTTAAGGTCTGGTCTCCACTTTTGCTGGTCTAATTATATGTTAAAATTATGAAGTTACATTTTTCTCTCATATAAATAAATAAAGTTATATTTCTCGGTATGCAGGCCTAGGTTTATATTTGGTTGCATTTGGATCATTAGTTTTTAAACTTGATATTTGAACTTTTTTGGTCTTAGTTAGTTCTTGAAGTTGACTTACTAAACATATTTTCTTTTCAAGTACTATAGGTAGGGGATTCAAACCACAAACTTTGTGGTTATGTCAGTTGAGTTATGCTCTCTTGGGCTGATATCAGTTAAATATTTTTTTAAAGTTGTTTAGGAAAGATATATCAATTATTGTTCTCTTCAAATTGGAAATTTTTTTTTCTTTATATATGTTTTCCTTTTTCTCTTTTCACTCTCCTTCACACTGCCTGCCTATTTTCTTCTTCTTTCCTTTCCTTTTTTTTTTCTTATCCAAACTTCTCGGTTCTTTGGTCCTTTGAACATTGTCACGTAGGAGACCTGTTCCAGATATGGAGTTGCATATCTGGAACATTGTGTTGTTTAAGATCTTGCATGTCTCATCAGCGCTATACCAACAAGATTAAAACCACTTGGAACATTTGGTTTAGTTTAAATCTCAAACTTCTTGGTCGAAGATGTAATATGCCTAAATATATTCAGCTACTTTAATTCCCTAACACCAATAGGTAGTATCTGAAAATTTAGCACCACCGAGCCAACTCTCACTGGAAATTGGCACTGCATTAAATTGACATGTTTTGTTTATAACCAGTGGATATTAGTTGGACACCTGAAAATTACGAGTAAGGTTTGGTTTTGACAATTATTTCGGTTTGGGCGGTTTTCTTGTTCACCCCTAGTAAAAATCTTTTAAAAAACCTCCCATATGTCACCTATTAACACAGTTGTACTGAGCCACAGCAAATCAGTAAAGGAGTAATTTATCATTCTTGGCAAGTTTGGGGTAGGAAATAAGTTTCGGTAGCTTGTAGACATGTTTATTCAGAAATTACGTGTAACTGAATTCTTAATCAGCTCCATATATAAGCTTTAAGAATTCAAGAAGTCTGAAGAAGAAACTAGATGAACAGAGAATGAAAATACAATTTTCAAACAGACGAGGGATATCTAAAAGTGTTCGAACTCTAAAGATAAAAGGTAAAAATCTTAAAAAGGTACCACCAAGAATTTGGTTAAAACAAAAAAACACTTCACAATTACTATTATTATTATTATTATTATTATTACTATTTAAGAAGCCTAGGCATAAAATGGCAATCCATTGGATTCTCAGTTTCTTATTTACTGTTTATGATGTTTCAAAACAAGTGCTCTCCATGTTTACCGTCTTTGGTGGTTTGCGTATATGCATATCAAAATTAAAGCTGAAGAAGACAAAATTACTCTTGATATTTTATTTTATATTTCATGTGAAACTTTGGAGTACAAGAAAGAAGAGTGTAGCTAAATCTATGCTTATAACTAAAACGAGAGAAAAAGAAACAAATATAAGGTACGAGAGTGTCTTAAAATGTCCTATAACCTTTTATAACAGGCAGCAGAAGAGAAAGCACTCCCTATTGGACCTGAAGAAGGAGGTTTTGGAATGTCATATGATCTCCTGTATGGGCGAGCTGGGTTCTTATGGGCTGCCTTATTCATTAATAAGCATCTTGGAGAGCAGAGAATACCTCATGATCTTCTCATGTCCGTAGTTGATGCTGTTTTAGCGGGTGGCAGGGCGGGTGCATCCGACAACCCAGACTGCCCTTTGATGTTCAGATGGCATGGGACGAGATATTATGGTGCAGCCAACGGCCTGGCTGGTATCTTGCAAGTACTTTTGCATTTCCCACTTTCAAAAGAGGACACTGATGATGTTAAGGGGACGTTGAGATATTTGATGAGCAAAAGATTTCCACACAGCGGTAACTATCCCTCAAGTGAAGGCAGCCCAAGGGACAAATTAGTGCAATGGTCTCATGGGGCCACTGGCATTGGCATTACCCTCTGCAAGGCTTCACAGGTTGGTTCATTCTTTCGTATCTAACTTTTTAATGTGGCTCAATGCTCATCTTTCGACTTCAATATCGTCCTTCTATTAGGCATGGAGGTTCTAGTATCTTTTTGTTGTTCAGTGTAGGGGTGAGGCAGATTTCTTAGTCTTGTTTATCCAGTGGAACACATATAAAACAGAAATCACGAAATCATGTTTACTAGGAAAGAAGTTCTTGGCCTTGATGTTTTAAGAAATTTTCAGATTTGTTGTCAACCATAGACCACAGCGATTAAGGTTAAGCTTCACTGGGAAAGGGTTGGATTAATTTAATAACTTCAATCTAGGATCTTTATTTTTCTATCATAAACTGCATAGTAAAGACTTATATTCCTGAATGAAATACATAAGGTGGTTACAGGAGTTCTGATTTTACTCGAATTTCAGGTTTTCCATGAAGACAGGGAGTTCAGGGACGCTGCAATTGAAGCAGGGGAAGTAGTATGGAAAAATGGATTGGTAAAGGAAGTTGGACTTGCTGACGGTGTTACCGGCAATGCATATGCCTTTCTTTCCCTTTATCGCCTTACCGGAGAGAGTGTCTATGAAGAAAGAGCCAGAGCATTTGGAACCTACCTGTATCATAATGCTAGCAAGCTTGTTGCTACCCAAAACCAGGTTGGAGGCGACCGTCTTTTCTCTCTTTTCCATGGACTAGCTGGAACTGCATGCCTTTGGTTCGATTTGCTTGAACCTGAGAAGTCAAGGTTCCCAGGTTATGAATTATAGTTGAAGCTCAACTCAAAAGCATCCCCTTTGGAGTATGAAGTTGTGTATATTATTCCACTGATGTCTTGATGTTTGGATCTTTTGGTGTGATGGACTCGTTAATAATCAATTGTACAGTGCCTAAGATGTGTTACGATAACACACCAATTGAAGTTCTAGAATTGGGTTTTCAAGAATGAAAACAATTCAAGGCAGGGGTGTTGGTTTCTTGACCTTGAAGTTTGAAATCACATTTCACGTGCTTGAATAAAAAAAAATTAATTGATTAAAAGAGAACTCACTGTTAATTCTCTGTCGTGGTGATTGTTTAATTATAAAAGGTAGGTTAACATTTGATACGTGGGAACTATAGAAGAGCCTATTGATTCTCACATTTTTTTAAAAAGAGGAACACTCTGTTCAAAACGAGTTTCTTCAGTTTTTATAATTTTAGCTATAATTTCTTTCATCGGATAGT

mRNA sequence

CACCATTGTTCAATAACACCATCGATTACAATAAAGAGAGCTCCCCACGTTGTTTCTTTGCTTTTCGAAAATTATAATTATAATTTGTTTATTTTCTTTCTTTCTTTTAGAAAAACTAAAACAACAAACGTCATTCGCGTGTGTCTACGGAAATAATAATTCAATTTATTATTCGTAAAAGCGCGTGTTTCACACTTGCAATCCCGACCCACCGTTTATGCTCTCCCCAACCGCCATTGATTTTCCCTTTTAACACCATCACTCCAAAACACAACTCACTACCCTCTCCCTCCCTCTCCAGATTTCATTTCAATTCCTTCTTCTTACACTAATGTCTTCCCTCCTCCACCTCGCTTCATCTCAGGAAGATCGAGAAGAAGGTGGTGGAGGAGGAGGAATGATCCAGATTATTCCACCCAACACTATTCTTCTTCCTTCTCAAACCTTTCTCTCTGCAGCCGTCTCTTTCAAGAATCAGGTTGTGGAGCTTACTTGGAAACAGAGAGGTAACGCGGATATTGGAGTTGATCCGACGGTGTATAACGGCTTACTTGGGACGGCGGTAACTTGCCTGAGATCGTACGAGGCCACCGGAAACCACCAGGACTTGGTATTGTGTTCTGATATTGTAGATAAATGTACGGTTCTAGCACGTGCCTCTCACAGGCACTTGACTTTTCTTTGTGGTAGAGGAGGAGTTTATGCTCTTGGCGCCGTGGTATCTCATTACATGAGGGACCCTCAAAAGCTTGACTTCTTCTTCAATCTTTTTCTTAAGGCAGCAGAAGAGAAAGCACTCCCTATTGGACCTGAAGAAGGAGGTTTTGGAATGTCATATGATCTCCTGTATGGGCGAGCTGGGTTCTTATGGGCTGCCTTATTCATTAATAAGCATCTTGGAGAGCAGAGAATACCTCATGATCTTCTCATGTCCGTAGTTGATGCTGTTTTAGCGGGTGGCAGGGCGGGTGCATCCGACAACCCAGACTGCCCTTTGATGTTCAGATGGCATGGGACGAGATATTATGGTGCAGCCAACGGCCTGGCTGGTATCTTGCAAGTACTTTTGCATTTCCCACTTTCAAAAGAGGACACTGATGATGTTAAGGGGACGTTGAGATATTTGATGAGCAAAAGATTTCCACACAGCGGTAACTATCCCTCAAGTGAAGGCAGCCCAAGGGACAAATTAGTGCAATGGTCTCATGGGGCCACTGGCATTGGCATTACCCTCTGCAAGGCTTCACAGGTTTTCCATGAAGACAGGGAGTTCAGGGACGCTGCAATTGAAGCAGGGGAAGTAGTATGGAAAAATGGATTGGTAAAGGAAGTTGGACTTGCTGACGGTGTTACCGGCAATGCATATGCCTTTCTTTCCCTTTATCGCCTTACCGGAGAGAGTGTCTATGAAGAAAGAGCCAGAGCATTTGGAACCTACCTGTATCATAATGCTAGCAAGCTTGTTGCTACCCAAAACCAGGTTGGAGGCGACCGTCTTTTCTCTCTTTTCCATGGACTAGCTGGAACTGCATGCCTTTGGTTCGATTTGCTTGAACCTGAGAAGTCAAGGTTCCCAGGTTATGAATTATAGTTGAAGCTCAACTCAAAAGCATCCCCTTTGGAGTATGAAGTTGTGTATATTATTCCACTGATGTCTTGATGTTTGGATCTTTTGGTGTGATGGACTCGTTAATAATCAATTGTACAGTGCCTAAGATGTGTTACGATAACACACCAATTGAAGTTCTAGAATTGGGTTTTCAAGAATGAAAACAATTCAAGGCAGGGGTGTTGGTTTCTTGACCTTGAAGTTTGAAATCACATTTCACGTGCTTGAATAAAAAAAAATTAATTGATTAAAAGAGAACTCACTGTTAATTCTCTGTCGTGGTGATTGTTTAATTATAAAAGGTAGGTTAACATTTGATACGTGGGAACTATAGAAGAGCCTATTGATTCTCACATTTTTTTAAAAAGAGGAACACTCTGTTCAAAACGAGTTTCTTCAGTTTTTATAATTTTAGCTATAATTTCTTTCATCGGATAGT

Coding sequence (CDS)

ATGTCTTCCCTCCTCCACCTCGCTTCATCTCAGGAAGATCGAGAAGAAGGTGGTGGAGGAGGAGGAATGATCCAGATTATTCCACCCAACACTATTCTTCTTCCTTCTCAAACCTTTCTCTCTGCAGCCGTCTCTTTCAAGAATCAGGTTGTGGAGCTTACTTGGAAACAGAGAGGTAACGCGGATATTGGAGTTGATCCGACGGTGTATAACGGCTTACTTGGGACGGCGGTAACTTGCCTGAGATCGTACGAGGCCACCGGAAACCACCAGGACTTGGTATTGTGTTCTGATATTGTAGATAAATGTACGGTTCTAGCACGTGCCTCTCACAGGCACTTGACTTTTCTTTGTGGTAGAGGAGGAGTTTATGCTCTTGGCGCCGTGGTATCTCATTACATGAGGGACCCTCAAAAGCTTGACTTCTTCTTCAATCTTTTTCTTAAGGCAGCAGAAGAGAAAGCACTCCCTATTGGACCTGAAGAAGGAGGTTTTGGAATGTCATATGATCTCCTGTATGGGCGAGCTGGGTTCTTATGGGCTGCCTTATTCATTAATAAGCATCTTGGAGAGCAGAGAATACCTCATGATCTTCTCATGTCCGTAGTTGATGCTGTTTTAGCGGGTGGCAGGGCGGGTGCATCCGACAACCCAGACTGCCCTTTGATGTTCAGATGGCATGGGACGAGATATTATGGTGCAGCCAACGGCCTGGCTGGTATCTTGCAAGTACTTTTGCATTTCCCACTTTCAAAAGAGGACACTGATGATGTTAAGGGGACGTTGAGATATTTGATGAGCAAAAGATTTCCACACAGCGGTAACTATCCCTCAAGTGAAGGCAGCCCAAGGGACAAATTAGTGCAATGGTCTCATGGGGCCACTGGCATTGGCATTACCCTCTGCAAGGCTTCACAGGTTTTCCATGAAGACAGGGAGTTCAGGGACGCTGCAATTGAAGCAGGGGAAGTAGTATGGAAAAATGGATTGGTAAAGGAAGTTGGACTTGCTGACGGTGTTACCGGCAATGCATATGCCTTTCTTTCCCTTTATCGCCTTACCGGAGAGAGTGTCTATGAAGAAAGAGCCAGAGCATTTGGAACCTACCTGTATCATAATGCTAGCAAGCTTGTTGCTACCCAAAACCAGGTTGGAGGCGACCGTCTTTTCTCTCTTTTCCATGGACTAGCTGGAACTGCATGCCTTTGGTTCGATTTGCTTGAACCTGAGAAGTCAAGGTTCCCAGGTTATGAATTATAG

Protein sequence

MSSLLHLASSQEDREEGGGGGGMIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL*
Homology
BLAST of CSPI04G04540 vs. ExPASy Swiss-Prot
Match: Q9FJN7 (LanC-like protein GCL1 OS=Arabidopsis thaliana OX=3702 GN=GCL1 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.6e-156
Identity = 262/395 (66.33%), Postives = 319/395 (80.76%), Query Frame = 0

Query: 31  TILLPSQTFLSAAVSFKNQVVELTWK------QRGNADIGVDPTVYNGLLGTAVTCLRSY 90
           TI LP+++FL AA   KNQVVE TWK        G+  + +DPTVY GLLGTA TCL+SY
Sbjct: 41  TISLPTESFLRAATLLKNQVVEATWKGGVEALASGSGPV-LDPTVYTGLLGTAFTCLKSY 100

Query: 91  EATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFF 150
           E T NHQDL+ C++I+D C  +ARA+ RH+TFLCGRGGV  LGA+V++Y  D  K DFF 
Sbjct: 101 EVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVANYRGDQSKRDFFL 160

Query: 151 NLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVD 210
            LFL+ AEE+ LP GPEEGGFGMSYDLLYGRAGFLWAALF+N++LG+  +P  LL  +V 
Sbjct: 161 GLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPIVA 220

Query: 211 AVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRY 270
           A+LAGGR GA+D+  CPL++R+HGTR++GAANGLAGIL VLLHFPLS+ED  DV+GTLRY
Sbjct: 221 AILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTLRY 280

Query: 271 LMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGITLCKASQVFHEDREFRDAAIEAGEV 330
           +MS RFP+SGNYP SEG+PRDKLVQW+HGATG+ ITL KASQVF ++R+FR+AAIEAGEV
Sbjct: 281 MMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAGEV 340

Query: 331 VWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYEERARAFGTYLYHNASKLVATQNQV 390
           VWK+GLVK+VGLADGV GNAYAFLSLYRLTG+ VYEERA+AF +YL  +A +LV   +Q 
Sbjct: 341 VWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTSQ- 400

Query: 391 GGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
             +  +SLF GLAG  CLWFDL+ P  S+FPGYE+
Sbjct: 401 ETEHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433

BLAST of CSPI04G04540 vs. ExPASy Swiss-Prot
Match: F4IEM5 (LanC-like protein GCR2 OS=Arabidopsis thaliana OX=3702 GN=GCR2 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.5e-95
Identity = 178/377 (47.21%), Postives = 240/377 (63.66%), Query Frame = 0

Query: 43  AVSFKNQVVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDK 102
           A+S K++VV  TW++ G      D  +Y G+LGTA    +SY+ T N  DL LC + V+ 
Sbjct: 51  ALSIKDKVVWETWERSGKR--VRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEA 110

Query: 103 CTVLARASHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEE 162
           C V +R S R +TF+CG  GV ALGAV +  + D Q  D +   F        LP     
Sbjct: 111 CDVASRDSER-VTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLP----- 170

Query: 163 GGFGMSYDLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPL 222
                 Y+LLYGRAG+LWA LF+NKH+G++ I  + + SVV+ +   GR    +   CPL
Sbjct: 171 ------YELLYGRAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQ-LGNKGTCPL 230

Query: 223 MFRWHGTRYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGS 282
           M+ WHG RY+GAA+GLAGI+ VL+H  L  ++  DVKGTL Y++  RFP SGNY SSEGS
Sbjct: 231 MYEWHGKRYWGAAHGLAGIMNVLMHTELEPDEIKDVKGTLSYMIQNRFP-SGNYLSSEGS 290

Query: 283 PRDKLVQWSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTG 342
             D+LV W HGA G+ +TL KA+QV++  +EF +AA+EAGEVVW  GL+K VG+  G++G
Sbjct: 291 KSDRLVHWCHGAPGVALTLVKAAQVYN-TKEFVEAAMEAGEVVWSRGLLKRVGICHGISG 350

Query: 343 NAYAFLSLYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACL 402
           N Y FLSLYRLT    Y  RA+AF ++L   + KL++     GGDR FSLF G+ G A +
Sbjct: 351 NTYVFLSLYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYM 410

Query: 403 WFDLLEPEKSRFPGYEL 420
             D+ +P ++ FPGYEL
Sbjct: 411 LLDMNDPTQALFPGYEL 410

BLAST of CSPI04G04540 vs. ExPASy Swiss-Prot
Match: Q8VZQ6 (LanC-like protein GCL2 OS=Arabidopsis thaliana OX=3702 GN=GCL2 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 9.6e-90
Identity = 179/410 (43.66%), Postives = 246/410 (60.00%), Query Frame = 0

Query: 12  EDREEGGGGGGMIQIIP-PNTILLPSQTFLSAAVSFKNQVVELTWKQRGNADIGVDPTVY 71
           +++E   GG  +  ++  P + L  SQ    +A+  K  VV  TW   G      D T+Y
Sbjct: 15  KEKESVSGGDTLRNLLAMPYSSL--SQQLKRSALDLKETVVIETWGFSGQT--VEDFTLY 74

Query: 72  NGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYALGAVV 131
           +G LG A    R+Y+ TGN  DL LC +IV  C   A AS   +TFLCGR GV  LGAV 
Sbjct: 75  SGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDT-ASASSGDVTFLCGRAGVCGLGAVA 134

Query: 132 SHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFINKHLG 191
           +    +   L+++   F        LP            +LLYGR G+LWA LFINK++G
Sbjct: 135 AKLSGEEDLLNYYLGQFRLIRLSSDLP-----------NELLYGRVGYLWACLFINKYIG 194

Query: 192 EQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAGILQVLLHFPL 251
           ++ +  D +  V   ++  GR+ A      PLMF W+G RY+GAA+GLAGI+ VL+   L
Sbjct: 195 KETLSSDTIREVAQEIIKEGRSMAKKG-SSPLMFEWYGKRYWGAAHGLAGIMHVLMDVQL 254

Query: 252 SKEDTDDVKGTLRYLMSKRFPHSGNYPSS-EGSPRDKLVQWSHGATGIGITLCKASQVFH 311
             ++ +DVKGTL+Y++  RFP SGNYP+S E   +D LV W HGA GI +TL KA++VF 
Sbjct: 255 KPDEAEDVKGTLKYMIKNRFP-SGNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEVFG 314

Query: 312 EDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYEERARAFGTY 371
           E REF +A+  A EVVW  GL+K VG+  G++GNAY FL+LYR TG S Y  RA+AF ++
Sbjct: 315 E-REFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAKAFASF 374

Query: 372 LYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           L     KL++     GGD  +SLF G+AG A L+ D+++P ++RFPGYEL
Sbjct: 375 LLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of CSPI04G04540 vs. ExPASy Swiss-Prot
Match: Q9JJK2 (LanC-like protein 2 OS=Mus musculus OX=10090 GN=Lancl2 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.1e-56
Identity = 131/367 (35.69%), Postives = 198/367 (53.95%), Query Frame = 0

Query: 66  DPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYA 125
           D + Y G  G A+  L+ Y  TG+   L+   D V +   L   S R +TFLCG  G  A
Sbjct: 99  DCSAYTGWTGIALLYLQLYRVTGDQTYLLRSLDYVKR--TLRNLSGRRVTFLCGDAGPLA 158

Query: 126 LGAVVSHYMRDPQKLDFFFNLFLK-----AAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 185
           +GAV+ H ++   +        L+       +E  LP            +LLYGRAG+L+
Sbjct: 159 VGAVIYHKLKSECESQECITKLLQMHRTIVCQESELP-----------DELLYGRAGYLY 218

Query: 186 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASD---NPDCPLMFRWHGTRYYGAANG 245
           A L++N  +G   +    +  VV A++  G++ + +   +  CPL+++WH  +Y GAA+G
Sbjct: 219 ALLYLNTEIGPGTVGETAIKEVVSAIIESGKSLSREERKSERCPLLYQWHRKQYVGAAHG 278

Query: 246 LAGILQVLLHFPLSKED----TDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHG 305
           +AGI  +L+  P +K D    T+ VK ++ Y+  K+F  SGNYPSS  +  D+LV W HG
Sbjct: 279 MAGIYYMLMQ-PEAKVDQETLTEMVKPSIDYVRHKKF-RSGNYPSSLSNETDRLVHWCHG 338

Query: 306 ATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLV-KEVGLADGVTGNAYAFLSLYR 365
           A G+   L +A QVF E++  ++ A+E  +V+W+ GL+ K  G+  G +GN Y+FLSLYR
Sbjct: 339 APGVIHVLLQAYQVFKEEKYLKE-AMECSDVIWQRGLLRKGYGICHGTSGNGYSFLSLYR 398

Query: 366 LTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKS 420
           LT +  Y  RA  F  +     +           DR +SLF G+AG      D+L PE +
Sbjct: 399 LTQDKKYLYRACKFAEWCLDYGAHGCRIP-----DRPYSLFEGMAGAVHFLSDILVPETA 444

BLAST of CSPI04G04540 vs. ExPASy Swiss-Prot
Match: F1MVX2 (Glutathione S-transferase LANCL1 OS=Bos taurus OX=9913 GN=LANCL1 PE=1 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-54
Identity = 135/361 (37.40%), Postives = 182/361 (50.42%), Query Frame = 0

Query: 66  DPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYA 125
           D TVY G  G AV  L  Y+  G+   L +    V +   L   S   +TFLCG GG  A
Sbjct: 58  DSTVYTGWAGIAVLYLHLYDVFGDPNYLQMAHGYVKQS--LNSLSKHSITFLCGDGGPLA 117

Query: 126 LGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFI 185
           + AVV H M + ++ +      +     K  P  P E        +LYGR G++ A LF+
Sbjct: 118 VAAVVHHKMNNEKQAEECITRLIHL--NKIDPHAPSE--------MLYGRMGYISALLFV 177

Query: 186 NKHLGEQRIPHDLLMSVVDAVLAGGRAGASD---NPDCPLMFRWHGTRYYGAANGLAGIL 245
           NK+ GE++IP   +  + + VL  G   A         PLM+ W+   Y GAA+GLAGI 
Sbjct: 178 NKNFGEEKIPQSHIQQICETVLTSGEDLARKRRFTGKTPLMYEWYQEYYVGAAHGLAGIY 237

Query: 246 QVLLHFPLSKEDT---DDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 305
             L+   L        + VK ++ Y+   +FP SGNYP      RD L+ W HGA G+  
Sbjct: 238 YYLMQPSLQVSHAKLHNLVKPSVDYVCQLKFP-SGNYPPCVDDSRDLLIHWCHGAPGVIY 297

Query: 306 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKE-VGLADGVTGNAYAFLSLYRLTGESV 365
            L +A +VF E+R   D A +  +V+W+ GL+K+  GL  G  GNAYAFLSLY LT ++ 
Sbjct: 298 MLTQAYKVFKEERYLND-AYQCADVIWQYGLLKKGYGLCHGTAGNAYAFLSLYSLTQDAK 357

Query: 366 YEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYE 420
           Y  RA  F  +          T      D  FSLF G+AGT     DLL P K+RFP +E
Sbjct: 358 YLYRACKFAEWCLDYGEHGCRTP-----DTPFSLFEGMAGTIYFLADLLVPTKARFPAFE 399

BLAST of CSPI04G04540 vs. ExPASy TrEMBL
Match: A0A0A0KZA8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026880 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.1e-249
Identity = 417/419 (99.52%), Postives = 418/419 (99.76%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN 60
           MSSLLHLASSQEDREEGGGGGG+IQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN
Sbjct: 1   MSSLLHLASSQEDREEGGGGGGIIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN 60

Query: 61  ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR 120
           ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR
Sbjct: 61  ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR 120

Query: 121 GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 180
           GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW
Sbjct: 121 GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 180

Query: 181 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG 240
           AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG
Sbjct: 181 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG 240

Query: 241 ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT 300
           ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT
Sbjct: 241 ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT 300

Query: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYE 360
           LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV GNAYAFLSLYRLTGESVYE
Sbjct: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVAGNAYAFLSLYRLTGESVYE 360

Query: 361 ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 419

BLAST of CSPI04G04540 vs. ExPASy TrEMBL
Match: A0A5D3D0W0 (LanC-like protein GCL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00050 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.4e-238
Identity = 402/420 (95.71%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPP-NTILLPSQTFLSAAVSFKNQVVELTWKQRG 60
           MSSLL LASSQEDRE GGGGGG IQI P  NTILLPSQTFLSAAVS KNQVVELTWK+RG
Sbjct: 1   MSSLLELASSQEDREGGGGGGG-IQIPPNCNTILLPSQTFLSAAVSLKNQVVELTWKKRG 60

Query: 61  NADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCG 120
           NADIGVDPTVYNGLLGTAVTCLRSYE TGNHQDLVLCSDIVDKCTV ARASHRHLTFLCG
Sbjct: 61  NADIGVDPTVYNGLLGTAVTCLRSYEVTGNHQDLVLCSDIVDKCTVAARASHRHLTFLCG 120

Query: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFL 180
           RGGVYALGAVVSHYMRDPQKLDFFFNLFL+AAEEKALP GPEEGGFGMSYDLLYGRAGFL
Sbjct: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLQAAEEKALPSGPEEGGFGMSYDLLYGRAGFL 180

Query: 181 WAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240
           WAALFINKHLGEQR+PHDLLMSV+DAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA
Sbjct: 181 WAALFINKHLGEQRLPHDLLMSVIDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240

Query: 241 GILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 300
           GILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+PRDKLVQWSHGATGIGI
Sbjct: 241 GILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNPRDKLVQWSHGATGIGI 300

Query: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVY 360
           TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV+GNAYAFLSLYRLTGESVY
Sbjct: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVSGNAYAFLSLYRLTGESVY 360

Query: 361 EERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           EERARAFGTYLYHNASKLVA QNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 EERARAFGTYLYHNASKLVANQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 419

BLAST of CSPI04G04540 vs. ExPASy TrEMBL
Match: A0A1S3BWM3 (lanC-like protein GCL1 OS=Cucumis melo OX=3656 GN=LOC103494424 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.4e-238
Identity = 402/420 (95.71%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPP-NTILLPSQTFLSAAVSFKNQVVELTWKQRG 60
           MSSLL LASSQEDRE GGGGGG IQI P  NTILLPSQTFLSAAVS KNQVVELTWK+RG
Sbjct: 1   MSSLLELASSQEDREGGGGGGG-IQIPPNCNTILLPSQTFLSAAVSLKNQVVELTWKKRG 60

Query: 61  NADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCG 120
           NADIGVDPTVYNGLLGTAVTCLRSYE TGNHQDLVLCSDIVDKCTV ARASHRHLTFLCG
Sbjct: 61  NADIGVDPTVYNGLLGTAVTCLRSYEVTGNHQDLVLCSDIVDKCTVAARASHRHLTFLCG 120

Query: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFL 180
           RGGVYALGAVVSHYMRDPQKLDFFFNLFL+AAEEKALP GPEEGGFGMSYDLLYGRAGFL
Sbjct: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLQAAEEKALPSGPEEGGFGMSYDLLYGRAGFL 180

Query: 181 WAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240
           WAALFINKHLGEQR+PHDLLMSV+DAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA
Sbjct: 181 WAALFINKHLGEQRLPHDLLMSVIDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240

Query: 241 GILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 300
           GILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+PRDKLVQWSHGATGIGI
Sbjct: 241 GILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNPRDKLVQWSHGATGIGI 300

Query: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVY 360
           TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV+GNAYAFLSLYRLTGESVY
Sbjct: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVSGNAYAFLSLYRLTGESVY 360

Query: 361 EERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           EERARAFGTYLYHNASKLVA QNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 EERARAFGTYLYHNASKLVANQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 419

BLAST of CSPI04G04540 vs. ExPASy TrEMBL
Match: A0A5A7TMJ7 (LanC-like protein GCL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004700 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 4.7e-233
Identity = 397/420 (94.52%), Postives = 403/420 (95.95%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPP-NTILLPSQTFLSAAVSFKNQVVELTWKQRG 60
           MSSLL LASSQEDRE GGGGGG IQI P  NTILLPSQTFLSAA      VVELTWK+RG
Sbjct: 1   MSSLLELASSQEDREGGGGGGG-IQIPPNCNTILLPSQTFLSAA------VVELTWKKRG 60

Query: 61  NADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCG 120
           NADIGVDPTVYNGLLGTAVTCLRSYE TGNHQDLVLCSDIVDKCTV ARASHRHLTFLCG
Sbjct: 61  NADIGVDPTVYNGLLGTAVTCLRSYEVTGNHQDLVLCSDIVDKCTVAARASHRHLTFLCG 120

Query: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFL 180
           RGGVYALGAVVSHYMRDPQKLDFFFNLFL+AAEEKALP GPEEGGFGMSYDLLYGRAGFL
Sbjct: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLQAAEEKALPSGPEEGGFGMSYDLLYGRAGFL 180

Query: 181 WAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240
           WAALFINKHLGEQR+PHDLLMSV+DAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA
Sbjct: 181 WAALFINKHLGEQRLPHDLLMSVIDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240

Query: 241 GILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 300
           GILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+PRDKLVQWSHGATGIGI
Sbjct: 241 GILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNPRDKLVQWSHGATGIGI 300

Query: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVY 360
           TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV+GNAYAFLSLYRLTGESVY
Sbjct: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVSGNAYAFLSLYRLTGESVY 360

Query: 361 EERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           EERARAFGTYLYHNASKLVA QNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 EERARAFGTYLYHNASKLVANQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 413

BLAST of CSPI04G04540 vs. ExPASy TrEMBL
Match: A0A6J1C4K3 (lanC-like protein GCL1 OS=Momordica charantia OX=3673 GN=LOC111008421 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.5e-210
Identity = 353/421 (83.85%), Postives = 380/421 (90.26%), Query Frame = 0

Query: 1   MSSLLHLASS--QEDREEGGGGGGMIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQR 60
           MSS+L  ASS  QE+RE        + +IPPN ILLP  TFL+AAVS KNQVVE+TWK+R
Sbjct: 1   MSSVLQFASSQPQEEREGAVPETEHLDLIPPNRILLPPHTFLAAAVSLKNQVVEVTWKRR 60

Query: 61  GNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLC 120
            N D+GVDPT Y GLLGTA+TCLRSYEATG+ QDL+LCSDIVD CT+ ARASHR LTFLC
Sbjct: 61  RNVDVGVDPTAYTGLLGTALTCLRSYEATGDQQDLLLCSDIVDACTLAARASHRQLTFLC 120

Query: 121 GRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGF 180
           GRGGV+ALGAVVSHYM D Q+LDFF NLFL+AA+EKALP+GPEEGG GMSYDLLYGRAGF
Sbjct: 121 GRGGVHALGAVVSHYMGDSQRLDFFLNLFLQAAQEKALPVGPEEGGLGMSYDLLYGRAGF 180

Query: 181 LWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGL 240
           LWAALF+N HLGEQR+PH  LMSVVDAVLAGGRAGASDN DCPLM+RWHGTRYYGAANGL
Sbjct: 181 LWAALFMNNHLGEQRLPHGFLMSVVDAVLAGGRAGASDNLDCPLMYRWHGTRYYGAANGL 240

Query: 241 AGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIG 300
           AGILQVLLHFPLSKED DDVKGTL+YLMSKRFPHSGNYPSSEG+ RDKLVQWSHGATG+G
Sbjct: 241 AGILQVLLHFPLSKEDADDVKGTLKYLMSKRFPHSGNYPSSEGNLRDKLVQWSHGATGMG 300

Query: 301 ITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESV 360
           ITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESV
Sbjct: 301 ITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESV 360

Query: 361 YEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYE 420
           YEERARAF T+LYHNASKLV  +N VG DR +SLFHGLAGTACLWFDL+EPEKSRFPGYE
Sbjct: 361 YEERARAFATFLYHNASKLVGPRNHVGADRSYSLFHGLAGTACLWFDLVEPEKSRFPGYE 420

BLAST of CSPI04G04540 vs. NCBI nr
Match: XP_004146876.1 (lanC-like protein GCL1 [Cucumis sativus] >XP_031745664.1 lanC-like protein GCL1 [Cucumis sativus] >XP_031745667.1 lanC-like protein GCL1 [Cucumis sativus] >KGN53211.1 hypothetical protein Csa_015397 [Cucumis sativus])

HSP 1 Score: 870.9 bits (2249), Expect = 4.4e-249
Identity = 417/419 (99.52%), Postives = 418/419 (99.76%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN 60
           MSSLLHLASSQEDREEGGGGGG+IQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN
Sbjct: 1   MSSLLHLASSQEDREEGGGGGGIIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN 60

Query: 61  ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR 120
           ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR
Sbjct: 61  ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR 120

Query: 121 GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 180
           GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW
Sbjct: 121 GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 180

Query: 181 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG 240
           AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG
Sbjct: 181 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG 240

Query: 241 ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT 300
           ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT
Sbjct: 241 ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT 300

Query: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYE 360
           LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV GNAYAFLSLYRLTGESVYE
Sbjct: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVAGNAYAFLSLYRLTGESVYE 360

Query: 361 ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 419

BLAST of CSPI04G04540 vs. NCBI nr
Match: XP_008453808.1 (PREDICTED: lanC-like protein GCL1 [Cucumis melo] >TYK16874.1 lanC-like protein GCL1 [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 1.7e-237
Identity = 402/420 (95.71%), Postives = 408/420 (97.14%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPP-NTILLPSQTFLSAAVSFKNQVVELTWKQRG 60
           MSSLL LASSQEDRE GGGGGG IQI P  NTILLPSQTFLSAAVS KNQVVELTWK+RG
Sbjct: 1   MSSLLELASSQEDREGGGGGGG-IQIPPNCNTILLPSQTFLSAAVSLKNQVVELTWKKRG 60

Query: 61  NADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCG 120
           NADIGVDPTVYNGLLGTAVTCLRSYE TGNHQDLVLCSDIVDKCTV ARASHRHLTFLCG
Sbjct: 61  NADIGVDPTVYNGLLGTAVTCLRSYEVTGNHQDLVLCSDIVDKCTVAARASHRHLTFLCG 120

Query: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFL 180
           RGGVYALGAVVSHYMRDPQKLDFFFNLFL+AAEEKALP GPEEGGFGMSYDLLYGRAGFL
Sbjct: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLQAAEEKALPSGPEEGGFGMSYDLLYGRAGFL 180

Query: 181 WAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240
           WAALFINKHLGEQR+PHDLLMSV+DAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA
Sbjct: 181 WAALFINKHLGEQRLPHDLLMSVIDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240

Query: 241 GILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 300
           GILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+PRDKLVQWSHGATGIGI
Sbjct: 241 GILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNPRDKLVQWSHGATGIGI 300

Query: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVY 360
           TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV+GNAYAFLSLYRLTGESVY
Sbjct: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVSGNAYAFLSLYRLTGESVY 360

Query: 361 EERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           EERARAFGTYLYHNASKLVA QNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 EERARAFGTYLYHNASKLVANQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 419

BLAST of CSPI04G04540 vs. NCBI nr
Match: KAA0044713.1 (lanC-like protein GCL1 [Cucumis melo var. makuwa])

HSP 1 Score: 816.6 bits (2108), Expect = 9.8e-233
Identity = 397/420 (94.52%), Postives = 403/420 (95.95%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPP-NTILLPSQTFLSAAVSFKNQVVELTWKQRG 60
           MSSLL LASSQEDRE GGGGGG IQI P  NTILLPSQTFLSAA      VVELTWK+RG
Sbjct: 1   MSSLLELASSQEDREGGGGGGG-IQIPPNCNTILLPSQTFLSAA------VVELTWKKRG 60

Query: 61  NADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCG 120
           NADIGVDPTVYNGLLGTAVTCLRSYE TGNHQDLVLCSDIVDKCTV ARASHRHLTFLCG
Sbjct: 61  NADIGVDPTVYNGLLGTAVTCLRSYEVTGNHQDLVLCSDIVDKCTVAARASHRHLTFLCG 120

Query: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFL 180
           RGGVYALGAVVSHYMRDPQKLDFFFNLFL+AAEEKALP GPEEGGFGMSYDLLYGRAGFL
Sbjct: 121 RGGVYALGAVVSHYMRDPQKLDFFFNLFLQAAEEKALPSGPEEGGFGMSYDLLYGRAGFL 180

Query: 181 WAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240
           WAALFINKHLGEQR+PHDLLMSV+DAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA
Sbjct: 181 WAALFINKHLGEQRLPHDLLMSVIDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLA 240

Query: 241 GILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGI 300
           GILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+PRDKLVQWSHGATGIGI
Sbjct: 241 GILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNPRDKLVQWSHGATGIGI 300

Query: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVY 360
           TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV+GNAYAFLSLYRLTGESVY
Sbjct: 301 TLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVSGNAYAFLSLYRLTGESVY 360

Query: 361 EERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           EERARAFGTYLYHNASKLVA QNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL
Sbjct: 361 EERARAFGTYLYHNASKLVANQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 413

BLAST of CSPI04G04540 vs. NCBI nr
Match: XP_038877665.1 (lanC-like protein GCL1 [Benincasa hispida])

HSP 1 Score: 791.6 bits (2043), Expect = 3.4e-225
Identity = 375/418 (89.71%), Postives = 399/418 (95.45%), Query Frame = 0

Query: 1   MSSLLHLASSQEDREEGGGGGGMIQIIPPNTILLPSQTFLSAAVSFKNQVVELTWKQRGN 60
           MSS+L LASSQ+DRE GGGGGG+   IP  +ILLP + FLSAAVS KNQVV++TW +R N
Sbjct: 1   MSSVLKLASSQDDREGGGGGGGI--EIPEKSILLPREAFLSAAVSLKNQVVDVTWNRRRN 60

Query: 61  ADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGR 120
            +IGVDPTVY+GLLGTAVTCLRSY+ATGNHQDL+LCSDIVDKCTV ARASHRHLTFLCGR
Sbjct: 61  EEIGVDPTVYSGLLGTAVTCLRSYKATGNHQDLLLCSDIVDKCTVAARASHRHLTFLCGR 120

Query: 121 GGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLW 180
           GGVYA+GAVVSHYM DPQKLDFFFNLFL+AA+EKALPIGPEEGGFGMSYDL+YGRAGFLW
Sbjct: 121 GGVYAIGAVVSHYMGDPQKLDFFFNLFLQAAQEKALPIGPEEGGFGMSYDLMYGRAGFLW 180

Query: 181 AALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAG 240
           AALFINKHLGEQR+PHDLLMSVVDAVLAGGRAGASDNPDCPLM+RWHGTRYYGAANGLAG
Sbjct: 181 AALFINKHLGEQRLPHDLLMSVVDAVLAGGRAGASDNPDCPLMYRWHGTRYYGAANGLAG 240

Query: 241 ILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGIT 300
           ILQVLLHFPLSKED DDVKGTLRYLMSKRFPHSGNYPSSEG+ RDKLVQWSHGATG+GIT
Sbjct: 241 ILQVLLHFPLSKEDADDVKGTLRYLMSKRFPHSGNYPSSEGNARDKLVQWSHGATGMGIT 300

Query: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYE 360
           LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGE+VYE
Sbjct: 301 LCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGETVYE 360

Query: 361 ERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYE 419
           ERARAFGTYLYH+ASKLVA +NQV GDRL+SLFHGLAGTACLWFDLLEPEKSRFPGYE
Sbjct: 361 ERARAFGTYLYHSASKLVAARNQVEGDRLYSLFHGLAGTACLWFDLLEPEKSRFPGYE 416

BLAST of CSPI04G04540 vs. NCBI nr
Match: KAE8637149.1 (hypothetical protein CSA_004631 [Cucumis sativus] >KAE8637170.1 hypothetical protein CSA_018801 [Cucumis sativus])

HSP 1 Score: 777.3 bits (2006), Expect = 6.6e-221
Identity = 369/370 (99.73%), Postives = 369/370 (99.73%), Query Frame = 0

Query: 50  VVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARA 109
           VVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARA
Sbjct: 9   VVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARA 68

Query: 110 SHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSY 169
           SHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSY
Sbjct: 69  SHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSY 128

Query: 170 DLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGT 229
           DLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGT
Sbjct: 129 DLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGT 188

Query: 230 RYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQ 289
           RYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQ
Sbjct: 189 RYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGSPRDKLVQ 248

Query: 290 WSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLS 349
           WSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGV GNAYAFLS
Sbjct: 249 WSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVAGNAYAFLS 308

Query: 350 LYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEP 409
           LYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEP
Sbjct: 309 LYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEP 368

Query: 410 EKSRFPGYEL 420
           EKSRFPGYEL
Sbjct: 369 EKSRFPGYEL 378

BLAST of CSPI04G04540 vs. TAIR 10
Match: AT5G65280.1 (GCR2-like 1 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.1e-157
Identity = 262/395 (66.33%), Postives = 319/395 (80.76%), Query Frame = 0

Query: 31  TILLPSQTFLSAAVSFKNQVVELTWK------QRGNADIGVDPTVYNGLLGTAVTCLRSY 90
           TI LP+++FL AA   KNQVVE TWK        G+  + +DPTVY GLLGTA TCL+SY
Sbjct: 41  TISLPTESFLRAATLLKNQVVEATWKGGVEALASGSGPV-LDPTVYTGLLGTAFTCLKSY 100

Query: 91  EATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFF 150
           E T NHQDL+ C++I+D C  +ARA+ RH+TFLCGRGGV  LGA+V++Y  D  K DFF 
Sbjct: 101 EVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVANYRGDQSKRDFFL 160

Query: 151 NLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVD 210
            LFL+ AEE+ LP GPEEGGFGMSYDLLYGRAGFLWAALF+N++LG+  +P  LL  +V 
Sbjct: 161 GLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPIVA 220

Query: 211 AVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRY 270
           A+LAGGR GA+D+  CPL++R+HGTR++GAANGLAGIL VLLHFPLS+ED  DV+GTLRY
Sbjct: 221 AILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTLRY 280

Query: 271 LMSKRFPHSGNYPSSEGSPRDKLVQWSHGATGIGITLCKASQVFHEDREFRDAAIEAGEV 330
           +MS RFP+SGNYP SEG+PRDKLVQW+HGATG+ ITL KASQVF ++R+FR+AAIEAGEV
Sbjct: 281 MMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAGEV 340

Query: 331 VWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYEERARAFGTYLYHNASKLVATQNQV 390
           VWK+GLVK+VGLADGV GNAYAFLSLYRLTG+ VYEERA+AF +YL  +A +LV   +Q 
Sbjct: 341 VWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTSQ- 400

Query: 391 GGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
             +  +SLF GLAG  CLWFDL+ P  S+FPGYE+
Sbjct: 401 ETEHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433

BLAST of CSPI04G04540 vs. TAIR 10
Match: AT1G52920.1 (G protein coupled receptor )

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-96
Identity = 178/377 (47.21%), Postives = 240/377 (63.66%), Query Frame = 0

Query: 43  AVSFKNQVVELTWKQRGNADIGVDPTVYNGLLGTAVTCLRSYEATGNHQDLVLCSDIVDK 102
           A+S K++VV  TW++ G      D  +Y G+LGTA    +SY+ T N  DL LC + V+ 
Sbjct: 51  ALSIKDKVVWETWERSGKR--VRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEA 110

Query: 103 CTVLARASHRHLTFLCGRGGVYALGAVVSHYMRDPQKLDFFFNLFLKAAEEKALPIGPEE 162
           C V +R S R +TF+CG  GV ALGAV +  + D Q  D +   F        LP     
Sbjct: 111 CDVASRDSER-VTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLP----- 170

Query: 163 GGFGMSYDLLYGRAGFLWAALFINKHLGEQRIPHDLLMSVVDAVLAGGRAGASDNPDCPL 222
                 Y+LLYGRAG+LWA LF+NKH+G++ I  + + SVV+ +   GR    +   CPL
Sbjct: 171 ------YELLYGRAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQ-LGNKGTCPL 230

Query: 223 MFRWHGTRYYGAANGLAGILQVLLHFPLSKEDTDDVKGTLRYLMSKRFPHSGNYPSSEGS 282
           M+ WHG RY+GAA+GLAGI+ VL+H  L  ++  DVKGTL Y++  RFP SGNY SSEGS
Sbjct: 231 MYEWHGKRYWGAAHGLAGIMNVLMHTELEPDEIKDVKGTLSYMIQNRFP-SGNYLSSEGS 290

Query: 283 PRDKLVQWSHGATGIGITLCKASQVFHEDREFRDAAIEAGEVVWKNGLVKEVGLADGVTG 342
             D+LV W HGA G+ +TL KA+QV++  +EF +AA+EAGEVVW  GL+K VG+  G++G
Sbjct: 291 KSDRLVHWCHGAPGVALTLVKAAQVYN-TKEFVEAAMEAGEVVWSRGLLKRVGICHGISG 350

Query: 343 NAYAFLSLYRLTGESVYEERARAFGTYLYHNASKLVATQNQVGGDRLFSLFHGLAGTACL 402
           N Y FLSLYRLT    Y  RA+AF ++L   + KL++     GGDR FSLF G+ G A +
Sbjct: 351 NTYVFLSLYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYM 410

Query: 403 WFDLLEPEKSRFPGYEL 420
             D+ +P ++ FPGYEL
Sbjct: 411 LLDMNDPTQALFPGYEL 410

BLAST of CSPI04G04540 vs. TAIR 10
Match: AT2G20770.1 (GCR2-like 2 )

HSP 1 Score: 332.0 bits (850), Expect = 6.8e-91
Identity = 179/410 (43.66%), Postives = 246/410 (60.00%), Query Frame = 0

Query: 12  EDREEGGGGGGMIQIIP-PNTILLPSQTFLSAAVSFKNQVVELTWKQRGNADIGVDPTVY 71
           +++E   GG  +  ++  P + L  SQ    +A+  K  VV  TW   G      D T+Y
Sbjct: 15  KEKESVSGGDTLRNLLAMPYSSL--SQQLKRSALDLKETVVIETWGFSGQT--VEDFTLY 74

Query: 72  NGLLGTAVTCLRSYEATGNHQDLVLCSDIVDKCTVLARASHRHLTFLCGRGGVYALGAVV 131
           +G LG A    R+Y+ TGN  DL LC +IV  C   A AS   +TFLCGR GV  LGAV 
Sbjct: 75  SGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDT-ASASSGDVTFLCGRAGVCGLGAVA 134

Query: 132 SHYMRDPQKLDFFFNLFLKAAEEKALPIGPEEGGFGMSYDLLYGRAGFLWAALFINKHLG 191
           +    +   L+++   F        LP            +LLYGR G+LWA LFINK++G
Sbjct: 135 AKLSGEEDLLNYYLGQFRLIRLSSDLP-----------NELLYGRVGYLWACLFINKYIG 194

Query: 192 EQRIPHDLLMSVVDAVLAGGRAGASDNPDCPLMFRWHGTRYYGAANGLAGILQVLLHFPL 251
           ++ +  D +  V   ++  GR+ A      PLMF W+G RY+GAA+GLAGI+ VL+   L
Sbjct: 195 KETLSSDTIREVAQEIIKEGRSMAKKG-SSPLMFEWYGKRYWGAAHGLAGIMHVLMDVQL 254

Query: 252 SKEDTDDVKGTLRYLMSKRFPHSGNYPSS-EGSPRDKLVQWSHGATGIGITLCKASQVFH 311
             ++ +DVKGTL+Y++  RFP SGNYP+S E   +D LV W HGA GI +TL KA++VF 
Sbjct: 255 KPDEAEDVKGTLKYMIKNRFP-SGNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEVFG 314

Query: 312 EDREFRDAAIEAGEVVWKNGLVKEVGLADGVTGNAYAFLSLYRLTGESVYEERARAFGTY 371
           E REF +A+  A EVVW  GL+K VG+  G++GNAY FL+LYR TG S Y  RA+AF ++
Sbjct: 315 E-REFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAKAFASF 374

Query: 372 LYHNASKLVATQNQVGGDRLFSLFHGLAGTACLWFDLLEPEKSRFPGYEL 420
           L     KL++     GGD  +SLF G+AG A L+ D+++P ++RFPGYEL
Sbjct: 375 LLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJN71.6e-15666.33LanC-like protein GCL1 OS=Arabidopsis thaliana OX=3702 GN=GCL1 PE=2 SV=1[more]
F4IEM51.5e-9547.21LanC-like protein GCR2 OS=Arabidopsis thaliana OX=3702 GN=GCR2 PE=1 SV=1[more]
Q8VZQ69.6e-9043.66LanC-like protein GCL2 OS=Arabidopsis thaliana OX=3702 GN=GCL2 PE=2 SV=1[more]
Q9JJK23.1e-5635.69LanC-like protein 2 OS=Mus musculus OX=10090 GN=Lancl2 PE=1 SV=1[more]
F1MVX21.3e-5437.40Glutathione S-transferase LANCL1 OS=Bos taurus OX=9913 GN=LANCL1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZA82.1e-24999.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026880 PE=3 SV=1[more]
A0A5D3D0W08.4e-23895.71LanC-like protein GCL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BWM38.4e-23895.71lanC-like protein GCL1 OS=Cucumis melo OX=3656 GN=LOC103494424 PE=3 SV=1[more]
A0A5A7TMJ74.7e-23394.52LanC-like protein GCL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... [more]
A0A6J1C4K32.5e-21083.85lanC-like protein GCL1 OS=Momordica charantia OX=3673 GN=LOC111008421 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004146876.14.4e-24999.52lanC-like protein GCL1 [Cucumis sativus] >XP_031745664.1 lanC-like protein GCL1 ... [more]
XP_008453808.11.7e-23795.71PREDICTED: lanC-like protein GCL1 [Cucumis melo] >TYK16874.1 lanC-like protein G... [more]
KAA0044713.19.8e-23394.52lanC-like protein GCL1 [Cucumis melo var. makuwa][more]
XP_038877665.13.4e-22589.71lanC-like protein GCL1 [Benincasa hispida][more]
KAE8637149.16.6e-22199.73hypothetical protein CSA_004631 [Cucumis sativus] >KAE8637170.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
AT5G65280.11.1e-15766.33GCR2-like 1 [more]
AT1G52920.11.1e-9647.21G protein coupled receptor [more]
AT2G20770.16.8e-9143.66GCR2-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020464LanC-like protein, eukaryoticPRINTSPR01951LANCEUKARYTEcoord: 199..214
score: 24.48
coord: 305..326
score: 18.18
coord: 267..282
score: 34.64
coord: 405..419
score: 58.06
IPR007822Lanthionine synthetase C-likePRINTSPR01950LANCSUPERcoord: 334..347
score: 42.3
coord: 66..84
score: 34.24
coord: 231..247
score: 51.02
coord: 389..406
score: 39.64
coord: 169..184
score: 53.44
coord: 287..307
score: 45.0
IPR007822Lanthionine synthetase C-likeSMARTSM01260LANC_like_2coord: 63..419
e-value: 6.8E-109
score: 377.7
IPR007822Lanthionine synthetase C-likePFAMPF05147LANC_likecoord: 66..419
e-value: 1.9E-83
score: 280.0
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 35..419
e-value: 3.4E-112
score: 377.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR12736LANC-LIKE PROTEINcoord: 29..419
NoneNo IPR availablePANTHERPTHR12736:SF14LANC-LIKE PROTEIN GCL1coord: 29..419
NoneNo IPR availableCDDcd04794euk_LANCLcoord: 69..415
e-value: 2.67356E-108
score: 321.198
NoneNo IPR availableSUPERFAMILY158745LanC-likecoord: 65..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04540.1CSPI04G04540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005886 plasma membrane