CSPI04G04490 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G04490
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTubulin-folding cofactor C
LocationChr4: 2917596 .. 2919823 (-)
RNA-Seq ExpressionCSPI04G04490
SyntenyCSPI04G04490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAACCCATTTTCCGTCATCGTTAAATTAAAAAACTCGCGGCTGCAACCGAAGCATCCATTAATGGCGGACGCTGATGGACCTTCGTCGATCCCTAATTCTATTCCTTCATATCCTTCAGACGCGAATCTCCAAAAGAAGCATGCTTCAGTCATCGAACGCCTAGCCAATCGCAATCAAACTCGCTTAGAGAATTCCATAACTCGCAGATCGGAATCCGATTCCTCTACCTCTTCCACTTCTTCCTTTCTCGACCGCTTTTCCGACTCCAAGCGCGCCATCGAGTCGGCACTCGCCCAATGCCGCCTCACACCGCCGGACCCCGCTCAACTCAGATCTCACCTCGATGGGATATCCACCTCAATCTCCGACCTCGAGAAGCTCGTTGCCGAATCCTCTTACTCTTTGCCCTCATACGAGGTACGAGCTTCACTCAAATCCATTTCGGAATTGAAACAAGCCCTCGATAATTTAAATTCTGAGTTGCTACCTAAAAAGAAATTCTCATTCAAAAACAAAGCCACGAAGAAAGACCAAAAATCAGAGTCGAAAGATCCAGGGCTTGAAAATGCTGATTCTATGTTAACGAACAAGCAACAGCAAGCGAGTTACAGCGCGCGGGATTCTCCAGGAATCAGAGACAAGGACGGAGAGATTCTGGTAAAGAATTTCAAGGGATCAGATGTTGGGGAATTCACTATCTCGGGTCTTAGTTCTTGTGAGGTCAAGTTGATCGGTAGTGTTAGGGCATTATTTATTCACAAACTAAGAAACTGCAAAGTTTACACAGGACCTGTAATGGGTTCAATTCTAATTGATGATGTTGAGGAATGTACCTTTGCAATGGCCTCCCATCAAATCCGGATTCACAACGCTAAGAAAAGCGATTTCTATCTCAGAGTCAGGAGTAGGCCGATCATTGAAGATAGTAGCAGCGTGCGATTCGCTCCCTATCGTGTGAGCTACGAAGGAATTGAAGAAGATCTCACAGATGCAACCCTTGGCGTGGAGACAGGGAATTGGGAAAATGTAGATGATTTTCGTTGGTTACGAGCCGTGCCGTCTCCGAACTGGTCGATTTTGCCGGAGGATGAGCGGATTGACACCATCAAAATCTCTCCCACAGAAGGTTAAGCCTTTGAAAGTTAAGTTTTTCATCTCTACTTTTGATGAATCCCAAGTTTTCTTTCAAGGTTTTGTTTGGTTAATAGAGCAGTAGGATGCATCATAGAGAATTTCAAGTAGTTGATTTTGCTGTGATTGTGTGTATACAAAACTGGGAATCCTAGTAAATTCTCTCCCTTTCAATTGGTTGATTTTTCAAAAAATATTTCCTGGGTTGGCTGTTTTTCTTGCTGCCTTCGAGTGAGTTGCTATAGCCACTTGATAACTTTGCCTGAAGGAATATGATTGTCATTTCTTTTCATACCATGAAGATTTAGACTACTATGATTTTGAATGAAACATTGTCTCAAAATTGAATATTAGTTCTGGAAACATGATTTGTAGGCTTTCTGCCCCTGTATGTATGCTTGTTGAATCTTGTAGCAGTCTCAAATTTTGATGTACTATGATGAGTAAAAAGTATTGGAGAGTTCTTCAATCAAAGGGGTGATCATAAGCACAACTGATATATGTCTCAAGAGTTTTCATCTAATGAATGCTTATGGAAGGTTTGGAAATTGCATGGAACAAATGAACACCAAGTACCTTATATGTAAGTTACGAACATGAAGTTCGAGAAGAAAGATATAAGCTATAAAGAAGAAGAAACATTCTTGAAAGTGTTGTCATTTCGTTCTCCTCAAAGATGTCAGAAGATCATACATAGATTATGGATATAGGAAATGATACCAACCAACCAACTCAAAGTTTTCCAAAGAGAACCAAAACCGATATGCAGAAGGACAATGCACGTTTTAGTGGCAAGAAATAGTCTCCATTCCTTTAGATTTATGATCCCCAACAACTCATACCGAAGCTTTCGTGATCGCATTCCAAATTTGAGCCAAAAGCTTTTGGTGCATCCCTCAAATATTGTATAAAGCAACATATTCATTTGTCTGCTTGTTCGTTTGCTAACAAGTATCCGTCGATATTACCTCCTGCCTAAAAAATTTAAGAGGAAACGAATGCCTCCGAGGAGATCATGGTTTGACTCGTTGTCATCGATTATACCCCATAATATTTATATTAAAACAACAACAAAATAAAAAGCATGGTTAGAG

mRNA sequence

CGCAACCCATTTTCCGTCATCGTTAAATTAAAAAACTCGCGGCTGCAACCGAAGCATCCATTAATGGCGGACGCTGATGGACCTTCGTCGATCCCTAATTCTATTCCTTCATATCCTTCAGACGCGAATCTCCAAAAGAAGCATGCTTCAGTCATCGAACGCCTAGCCAATCGCAATCAAACTCGCTTAGAGAATTCCATAACTCGCAGATCGGAATCCGATTCCTCTACCTCTTCCACTTCTTCCTTTCTCGACCGCTTTTCCGACTCCAAGCGCGCCATCGAGTCGGCACTCGCCCAATGCCGCCTCACACCGCCGGACCCCGCTCAACTCAGATCTCACCTCGATGGGATATCCACCTCAATCTCCGACCTCGAGAAGCTCGTTGCCGAATCCTCTTACTCTTTGCCCTCATACGAGGTACGAGCTTCACTCAAATCCATTTCGGAATTGAAACAAGCCCTCGATAATTTAAATTCTGAGTTGCTACCTAAAAAGAAATTCTCATTCAAAAACAAAGCCACGAAGAAAGACCAAAAATCAGAGTCGAAAGATCCAGGGCTTGAAAATGCTGATTCTATGTTAACGAACAAGCAACAGCAAGCGAGTTACAGCGCGCGGGATTCTCCAGGAATCAGAGACAAGGACGGAGAGATTCTGGTAAAGAATTTCAAGGGATCAGATGTTGGGGAATTCACTATCTCGGGTCTTAGTTCTTGTGAGGTCAAGTTGATCGGTAGTGTTAGGGCATTATTTATTCACAAACTAAGAAACTGCAAAGTTTACACAGGACCTGTAATGGGTTCAATTCTAATTGATGATGTTGAGGAATGTACCTTTGCAATGGCCTCCCATCAAATCCGGATTCACAACGCTAAGAAAAGCGATTTCTATCTCAGAGTCAGGAGTAGGCCGATCATTGAAGATAGTAGCAGCGTGCGATTCGCTCCCTATCGTGTGAGCTACGAAGGAATTGAAGAAGATCTCACAGATGCAACCCTTGGCGTGGAGACAGGGAATTGGGAAAATGTAGATGATTTTCGTTGGTTACGAGCCGTGCCGTCTCCGAACTGGTCGATTTTGCCGGAGGATGAGCGGATTGACACCATCAAAATCTCTCCCACAGAAGGTTAAGCCTTTGAAAGTTAAGTTTTTCATCTCTACTTTTGATGAATCCCAAGTTTTCTTTCAAGGTTTTGTTTGGTTAATAGAGCAGTAGGATGCATCATAGAGAATTTCAAGTAGTTGATTTTGCTGTGATTGTGTGTATACAAAACTGGGAATCCTAGTAAATTCTCTCCCTTTCAATTGGTTGATTTTTCAAAAAATATTTCCTGGGTTGGCTGTTTTTCTTGCTGCCTTCGAGTGAGTTGCTATAGCCACTTGATAACTTTGCCTGAAGGAATATGATTGTCATTTCTTTTCATACCATGAAGATTTAGACTACTATGATTTTGAATGAAACATTGTCTCAAAATTGAATATTAGTTCTGGAAACATGATTTGTAGGCTTTCTGCCCCTGTATGTATGCTTGTTGAATCTTGTAGCAGTCTCAAATTTTGATGTACTATGATGAGTAAAAAGTATTGGAGAGTTCTTCAATCAAAGGGGTGATCATAAGCACAACTGATATATGTCTCAAGAGTTTTCATCTAATGAATGCTTATGGAAGGTTTGGAAATTGCATGGAACAAATGAACACCAAGTACCTTATATGTAAGTTACGAACATGAAGTTCGAGAAGAAAGATATAAGCTATAAAGAAGAAGAAACATTCTTGAAAGTGTTGTCATTTCGTTCTCCTCAAAGATGTCAGAAGATCATACATAGATTATGGATATAGGAAATGATACCAACCAACCAACTCAAAGTTTTCCAAAGAGAACCAAAACCGATATGCAGAAGGACAATGCACGTTTTAGTGGCAAGAAATAGTCTCCATTCCTTTAGATTTATGATCCCCAACAACTCATACCGAAGCTTTCGTGATCGCATTCCAAATTTGAGCCAAAAGCTTTTGGTGCATCCCTCAAATATTGTATAAAGCAACATATTCATTTGTCTGCTTGTTCGTTTGCTAACAAGTATCCGTCGATATTACCTCCTGCCTAAAAAATTTAAGAGGAAACGAATGCCTCCGAGGAGATCATGGTTTGACTCGTTGTCATCGATTATACCCCATAATATTTATATTAAAACAACAACAAAATAAAAAGCATGGTTAGAG

Coding sequence (CDS)

ATGGCGGACGCTGATGGACCTTCGTCGATCCCTAATTCTATTCCTTCATATCCTTCAGACGCGAATCTCCAAAAGAAGCATGCTTCAGTCATCGAACGCCTAGCCAATCGCAATCAAACTCGCTTAGAGAATTCCATAACTCGCAGATCGGAATCCGATTCCTCTACCTCTTCCACTTCTTCCTTTCTCGACCGCTTTTCCGACTCCAAGCGCGCCATCGAGTCGGCACTCGCCCAATGCCGCCTCACACCGCCGGACCCCGCTCAACTCAGATCTCACCTCGATGGGATATCCACCTCAATCTCCGACCTCGAGAAGCTCGTTGCCGAATCCTCTTACTCTTTGCCCTCATACGAGGTACGAGCTTCACTCAAATCCATTTCGGAATTGAAACAAGCCCTCGATAATTTAAATTCTGAGTTGCTACCTAAAAAGAAATTCTCATTCAAAAACAAAGCCACGAAGAAAGACCAAAAATCAGAGTCGAAAGATCCAGGGCTTGAAAATGCTGATTCTATGTTAACGAACAAGCAACAGCAAGCGAGTTACAGCGCGCGGGATTCTCCAGGAATCAGAGACAAGGACGGAGAGATTCTGGTAAAGAATTTCAAGGGATCAGATGTTGGGGAATTCACTATCTCGGGTCTTAGTTCTTGTGAGGTCAAGTTGATCGGTAGTGTTAGGGCATTATTTATTCACAAACTAAGAAACTGCAAAGTTTACACAGGACCTGTAATGGGTTCAATTCTAATTGATGATGTTGAGGAATGTACCTTTGCAATGGCCTCCCATCAAATCCGGATTCACAACGCTAAGAAAAGCGATTTCTATCTCAGAGTCAGGAGTAGGCCGATCATTGAAGATAGTAGCAGCGTGCGATTCGCTCCCTATCGTGTGAGCTACGAAGGAATTGAAGAAGATCTCACAGATGCAACCCTTGGCGTGGAGACAGGGAATTGGGAAAATGTAGATGATTTTCGTTGGTTACGAGCCGTGCCGTCTCCGAACTGGTCGATTTTGCCGGAGGATGAGCGGATTGACACCATCAAAATCTCTCCCACAGAAGGTTAA

Protein sequence

MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTSSFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVYTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSYEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG*
Homology
BLAST of CSPI04G04490 vs. ExPASy Swiss-Prot
Match: Q9SMR2 (Tubulin-folding cofactor C OS=Arabidopsis thaliana OX=3702 GN=TFCC PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 7.6e-104
Identity = 208/350 (59.43%), Postives = 263/350 (75.14%), Query Frame = 0

Query: 5   DGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTSSFLD 64
           DG SS+  ++     D  LQKKH  ++ERL+ R+Q R  +S    S S S+  STSSFL 
Sbjct: 4   DGQSSVAETL-----DPALQKKHHDMLERLSARHQARKSDSPDSSSSSSSTLESTSSFLA 63

Query: 65  RFSDSKRAIESALAQCRL--TPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRA 124
           +FSDSKR+IES +A+ RL  +  D ++L+S L  IS +I +LEKL+AE+SY LPSYEVR+
Sbjct: 64  KFSDSKRSIESRIAESRLASSSTDSSKLKSDLAEISVAIDNLEKLLAENSYFLPSYEVRS 123

Query: 125 SLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQAS 184
           SLK +S+LKQ+LD L+ EL+PKKKFSFK+K+T K  K ESK P ++  D +L  K     
Sbjct: 124 SLKIVSDLKQSLDILSGELVPKKKFSFKSKSTTK--KPESKLPEIQKPDVVLPPK----L 183

Query: 185 YSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVYT 244
              RDSPG+R+K GE LVK+F+GS +GEFT+S L SC+VKL G+V ALF+H+L+ C VYT
Sbjct: 184 VPVRDSPGLRNKHGETLVKSFEGSSIGEFTLSDLDSCQVKLTGTVNALFLHRLKKCSVYT 243

Query: 245 GPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSYE 304
           GPV+GSILIDDVE+C   +ASHQIRIH A+KSDFYLRVRSRPIIEDS+ VRFAPY + Y+
Sbjct: 244 GPVIGSILIDDVEDCVLVLASHQIRIHCARKSDFYLRVRSRPIIEDSNGVRFAPYCLDYK 303

Query: 305 GIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKIS 353
           GI+EDL  A L  ET NW NVDDFRWLRAV SPNWS+LPE+ER+  + IS
Sbjct: 304 GIDEDLKTAGLEEETNNWANVDDFRWLRAVQSPNWSLLPEEERVSLLTIS 342

BLAST of CSPI04G04490 vs. ExPASy Swiss-Prot
Match: Q8VCN9 (Tubulin-specific chaperone C OS=Mus musculus OX=10090 GN=Tbcc PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 5.0e-39
Identity = 114/352 (32.39%), Postives = 177/352 (50.28%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTR---LENSITRRSESDSSTS 60
           M   D   ++ ++    P D +L      V ERL  R Q R   +E    +R + +    
Sbjct: 1   MEGVDCSMALADAAAGSPRDLSL------VPERLQRREQERQIEVERRKQKRQDQEVEEE 60

Query: 61  STSSFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPS 120
            +  F   F+  + A+E  L                L+  +  +  L KL+ +S   L +
Sbjct: 61  KSGFFAAAFARERAAVEELLR--------GEASAERLEEAANRLQGLRKLLNDSVLFLAA 120

Query: 121 YEVRASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESK---DPGLENADSML 180
           Y++R    ++++L+  L     EL PKK+F+F  KA KKD    ++    P    A S  
Sbjct: 121 YDLRQGQAALAQLQAVLTERRQELQPKKRFAF--KARKKDAAGTAQVDAAPVTSAAPSPP 180

Query: 181 TNKQQQASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHK 240
             K+++ +  A  + G  + + + L K  +     +  +S L++C VKL G+   L + K
Sbjct: 181 VTKEEEGAPGASWACGFSNLESQDLEKRAEELHQRDVLLSDLTNCTVKLCGNPNTLRLAK 240

Query: 241 LRNCKVYTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRF 300
            R CKV  GPV  S+ ++D  +C  A+A  Q+R+H  K +  +L+V SR I+ED S ++F
Sbjct: 241 ARGCKVLCGPVTTSVFLEDCRDCVLAVACQQLRVHTTKDTRVFLQVTSRAIVEDCSGIQF 300

Query: 301 APYRVSYEGIEEDLTDATLGVETGNWENVDDFRWL-RAVPSPNWSILPEDER 346
           APY  SY GI++D  D+ L     NW+ VDDF WL R V SPNWSILPE+ER
Sbjct: 301 APYTWSYPGIDKDFQDSGLDRSKNNWDQVDDFNWLARNVASPNWSILPEEER 336

BLAST of CSPI04G04490 vs. ExPASy Swiss-Prot
Match: Q5R5J7 (Tubulin-specific chaperone C OS=Pongo abelii OX=9601 GN=TBCC PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.2e-37
Identity = 106/345 (30.72%), Postives = 177/345 (51.30%), Query Frame = 0

Query: 12  NSIPSYPSDANLQKKHASVIERLANRNQTR---LENSITRRSESDSSTSSTSSFLDRFSD 71
           ++ P    D   Q+  + V ERL  R Q R   +E    +R   +    ++  F   F+ 
Sbjct: 7   SAAPVRSGDMESQRDMSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFAATFAR 66

Query: 72  SKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRASLKSIS 131
            + A+E  L        + A+    L+  ++ +  L+KL+ +S + L +Y++R   ++++
Sbjct: 67  ERAAVEELL--------ERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALA 126

Query: 132 ELKQALDNLNSELLPKKKFSFKNKATKKDQKSESK-------DPGLENADSMLTNKQQQA 191
            L+ AL     EL PKK+F+FK +   KD  S +K        P +E+       K+ + 
Sbjct: 127 RLQAALAERRRELQPKKRFAFKTRG--KDAASCTKVDAAPGIPPAVESIQDSPLPKKAEG 186

Query: 192 SYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVY 251
              +    G  + + ++L K        +  ++ LS+C V+L G+   L + K  +CK+ 
Sbjct: 187 DLGSSWLCGFSNLESQVLEKRASELHQRDVLLTELSNCTVRLYGNPNTLRLTKAHSCKLL 246

Query: 252 TGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSY 311
            GPV  S+ ++D  +C  A+A  Q+RIH+ K +  +L+V SR I+ED S ++FAPY  SY
Sbjct: 247 CGPVSTSVFLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAIVEDCSGIQFAPYTWSY 306

Query: 312 EGIEEDLTDATLGVETGNWENVDDFRWL-RAVPSPNWSILPEDER 346
             I++D   + L     NW +VDDF WL R + SPNW ILPE+ER
Sbjct: 307 PEIDKDFESSGLDRSKNNWNDVDDFNWLARDMASPNWCILPEEER 341

BLAST of CSPI04G04490 vs. ExPASy Swiss-Prot
Match: Q15814 (Tubulin-specific chaperone C OS=Homo sapiens OX=9606 GN=TBCC PE=1 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 2.8e-37
Identity = 105/337 (31.16%), Postives = 173/337 (51.34%), Query Frame = 0

Query: 20  DANLQKKHASVIERLANRNQTR---LENSITRRSESDSSTSSTSSFLDRFSDSKRAIESA 79
           D   Q+  + V ERL  R Q R   +E    +R   +    ++  F+  F   + A+E  
Sbjct: 15  DMESQRDLSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFVATFVRERAAVEEL 74

Query: 80  LAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRASLKSISELKQALDN 139
           L        + A+    L+  ++ +  L+KL+ +S + L +Y++R   ++++ L+ AL  
Sbjct: 75  L--------ERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALARLQAALAE 134

Query: 140 LNSELLPKKKFSFKNKATKKDQKSESK-------DPGLENADSMLTNKQQQASYSARDSP 199
               L PKK+F+FK +   KD  S +K        P +E+       K+ +         
Sbjct: 135 RRRGLQPKKRFAFKTRG--KDAASSTKVDAAPGIPPAVESIQDSPLPKKAEGDLGPSWVC 194

Query: 200 GIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVYTGPVMGSI 259
           G  + + ++L K        +  ++ LS+C V+L G+   L + K  +CK+  GPV  S+
Sbjct: 195 GFSNLESQVLEKRASELHQRDVLLTELSNCTVRLYGNPNTLRLTKAHSCKLLCGPVSTSV 254

Query: 260 LIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSYEGIEEDLT 319
            ++D  +C  A+A  Q+RIH+ K +  +L+V SR I+ED S ++FAPY  SY  I++D  
Sbjct: 255 FLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAIVEDCSGIQFAPYTWSYPEIDKDFE 314

Query: 320 DATLGVETGNWENVDDFRWL-RAVPSPNWSILPEDER 346
            + L     NW +VDDF WL R + SPNWSILPE+ER
Sbjct: 315 SSGLDRSKNNWNDVDDFNWLARDMASPNWSILPEEER 341

BLAST of CSPI04G04490 vs. ExPASy Swiss-Prot
Match: Q3SZE9 (Tubulin-specific chaperone C OS=Bos taurus OX=9913 GN=TBCC PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.3e-36
Identity = 101/336 (30.06%), Postives = 173/336 (51.49%), Query Frame = 0

Query: 20  DANLQKKHASVIERLANR---NQTRLENSITRRSESDSSTSSTSSFLDRFSDSKRAIESA 79
           D   Q++   V ERL  R    Q  +E    +R + +     +  F   F+  + A+E  
Sbjct: 15  DLGSQRERTLVPERLQKREHERQLEVERRKQKRQDQEVEEEKSDFFAAAFARERSAVEEL 74

Query: 80  LAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRASLKSISELKQALDN 139
           L        +  +    L+  +  +  L+KL+ +S   L +Y++R + + ++ L+ AL  
Sbjct: 75  L--------ESGESVERLEEAAARLQGLQKLINDSVLFLAAYDLRQAQEVLARLQAALAK 134

Query: 140 LNSELLPKKKFSFKNKATKKDQKSESK---DPGLENADSMLTNK---QQQASYSARDSPG 199
              EL PKK+F+FK +  KKD  S ++    P    A+  LT+    +++  + +    G
Sbjct: 135 RRQELQPKKRFAFKTR--KKDAASATQVASAPDAPAAEGSLTSPPPLKEEGDFDSSWICG 194

Query: 200 IRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVYTGPVMGSIL 259
             +   ++L K  +     +  ++ L +C +KL G+   L + K + C +  GPV  S+ 
Sbjct: 195 FSNLQSQVLEKRAEELHQQDVLLTQLRNCTIKLYGNPNTLRLTKAQGCTLLCGPVSTSVF 254

Query: 260 IDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSYEGIEEDLTD 319
           ++D  +C  A+A  Q+R+H  K +  +L+V SR I+ED + ++FAPY  SY GI++D   
Sbjct: 255 LEDCSDCVLAVACQQLRVHTTKDTRIFLQVTSRAIMEDCTGIQFAPYTWSYPGIDKDFEG 314

Query: 320 ATLGVETGNWENVDDFRWL-RAVPSPNWSILPEDER 346
           + L     NW +VDDF WL R V SPNW++LPE+ER
Sbjct: 315 SGLDKNKNNWNDVDDFNWLARDVASPNWNVLPEEER 340

BLAST of CSPI04G04490 vs. ExPASy TrEMBL
Match: A0A0A0KZA3 (Tubulin-folding cofactor C OS=Cucumis sativus OX=3659 GN=Csa_4G026830 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 5.5e-190
Identity = 353/356 (99.16%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADAD PSSIPNSIPS PSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADEPSSIPNSIPSDPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSML NKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLMNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV
Sbjct: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG
Sbjct: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 356

BLAST of CSPI04G04490 vs. ExPASy TrEMBL
Match: A0A5D3D2T6 (Tubulin-folding cofactor C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00100 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 5.9e-184
Identity = 344/356 (96.63%), Postives = 348/356 (97.75%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADADG SSIPNSIPS PSDANLQKKHASVIERLA RNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADGASSIPNSIPSDPSDANLQKKHASVIERLAIRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGL NADSMLTNKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLGNADSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY+ARDSPGIRDKDGEILVKNFKGSD+GEFTISGLSSCEVKLIG+VRALFIHKLRNCKV
Sbjct: 181 ASYNARDSPGIRDKDGEILVKNFKGSDIGEFTISGLSSCEVKLIGTVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVEECTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDA+LGVETGNWENVDDF WLRAVPSPNWSILPEDERIDTIKIS TEG
Sbjct: 301 YEGIEEDLTDASLGVETGNWENVDDFLWLRAVPSPNWSILPEDERIDTIKISLTEG 356

BLAST of CSPI04G04490 vs. ExPASy TrEMBL
Match: A0A1S3BX62 (Tubulin-folding cofactor C OS=Cucumis melo OX=3656 GN=LOC103494429 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 5.9e-184
Identity = 344/356 (96.63%), Postives = 348/356 (97.75%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADADG SSIPNSIPS PSDANLQKKHASVIERLA RNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADGASSIPNSIPSDPSDANLQKKHASVIERLAIRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGL NADSMLTNKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLGNADSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY+ARDSPGIRDKDGEILVKNFKGSD+GEFTISGLSSCEVKLIG+VRALFIHKLRNCKV
Sbjct: 181 ASYNARDSPGIRDKDGEILVKNFKGSDIGEFTISGLSSCEVKLIGTVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVEECTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDA+LGVETGNWENVDDF WLRAVPSPNWSILPEDERIDTIKIS TEG
Sbjct: 301 YEGIEEDLTDASLGVETGNWENVDDFLWLRAVPSPNWSILPEDERIDTIKISLTEG 356

BLAST of CSPI04G04490 vs. ExPASy TrEMBL
Match: A0A5A7TP94 (Tubulin-folding cofactor C OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004650 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 343/356 (96.35%), Postives = 348/356 (97.75%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADADG SSIPNSIPS PSDANLQKKHASVIERLA RNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADGASSIPNSIPSDPSDANLQKKHASVIERLAIRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGL NADSMLTNKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLGNADSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY+ARDSPGIRDKDGEILVKNFKGSD+GEFTISGLSSCEVKLIG+VRALFIHKLRNCKV
Sbjct: 181 ASYNARDSPGIRDKDGEILVKNFKGSDIGEFTISGLSSCEVKLIGTVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDV+ECTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVQECTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDA+LGVETGNWENVDDF WLRAVPSPNWSILPEDERIDTIKIS TEG
Sbjct: 301 YEGIEEDLTDASLGVETGNWENVDDFLWLRAVPSPNWSILPEDERIDTIKISLTEG 356

BLAST of CSPI04G04490 vs. ExPASy TrEMBL
Match: A0A6J1EMN1 (Tubulin-folding cofactor C OS=Cucurbita moschata OX=3662 GN=LOC111435895 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.7e-170
Identity = 320/356 (89.89%), Postives = 334/356 (93.82%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MAD DG SSIPNSIP +PSD++L KKHASVIERLANRNQTRLENSITRRSESDSS+SSTS
Sbjct: 1   MADDDGASSIPNSIPPHPSDSSLHKKHASVIERLANRNQTRLENSITRRSESDSSSSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIES LAQCRLTPP+P+Q+RSHLDGIS SISDLEKLVAE+SYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESDLAQCRLTPPEPSQVRSHLDGISASISDLEKLVAETSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQ+L++LNSELLPKKKFSFKNK TKKD KSESKD GL NA SMLTNKQQQ
Sbjct: 121 RASLKSISELKQSLEDLNSELLPKKKFSFKNKGTKKDTKSESKDLGLGNAGSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY ARDSPGIRDKDGE+LVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV
Sbjct: 181 ASYIARDSPGIRDKDGEVLVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVE CTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPY VS
Sbjct: 241 YTGPVMGSILIDDVEGCTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYCVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDL DA+LG ETGNWENVDDFRWLRAV SPNWSIL E ERIDT+KIS TEG
Sbjct: 301 YEGIEEDLKDASLGEETGNWENVDDFRWLRAVQSPNWSILAEGERIDTVKISSTEG 356

BLAST of CSPI04G04490 vs. NCBI nr
Match: XP_004146878.1 (tubulin-folding cofactor C [Cucumis sativus] >XP_031745695.1 tubulin-folding cofactor C-like [Cucumis sativus] >XP_031745696.1 tubulin-folding cofactor C-like [Cucumis sativus] >XP_031745718.1 tubulin-folding cofactor C-like [Cucumis sativus] >KAE8637155.1 hypothetical protein CSA_004637 [Cucumis sativus] >KAE8637175.1 hypothetical protein CSA_018806 [Cucumis sativus] >KAE8637238.1 hypothetical protein CSA_021633 [Cucumis sativus] >KGN53206.1 hypothetical protein Csa_015020 [Cucumis sativus])

HSP 1 Score: 673.3 bits (1736), Expect = 1.1e-189
Identity = 353/356 (99.16%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADAD PSSIPNSIPS PSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADEPSSIPNSIPSDPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSML NKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLMNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV
Sbjct: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG
Sbjct: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 356

BLAST of CSPI04G04490 vs. NCBI nr
Match: XP_008453815.1 (PREDICTED: tubulin-folding cofactor C [Cucumis melo] >TYK16879.1 tubulin-folding cofactor C [Cucumis melo var. makuwa])

HSP 1 Score: 653.3 bits (1684), Expect = 1.2e-183
Identity = 344/356 (96.63%), Postives = 348/356 (97.75%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADADG SSIPNSIPS PSDANLQKKHASVIERLA RNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADGASSIPNSIPSDPSDANLQKKHASVIERLAIRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGL NADSMLTNKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLGNADSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY+ARDSPGIRDKDGEILVKNFKGSD+GEFTISGLSSCEVKLIG+VRALFIHKLRNCKV
Sbjct: 181 ASYNARDSPGIRDKDGEILVKNFKGSDIGEFTISGLSSCEVKLIGTVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVEECTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDA+LGVETGNWENVDDF WLRAVPSPNWSILPEDERIDTIKIS TEG
Sbjct: 301 YEGIEEDLTDASLGVETGNWENVDDFLWLRAVPSPNWSILPEDERIDTIKISLTEG 356

BLAST of CSPI04G04490 vs. NCBI nr
Match: KAA0044708.1 (tubulin-folding cofactor C [Cucumis melo var. makuwa])

HSP 1 Score: 652.1 bits (1681), Expect = 2.7e-183
Identity = 343/356 (96.35%), Postives = 348/356 (97.75%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MADADG SSIPNSIPS PSDANLQKKHASVIERLA RNQTRLENSITRRSESDSSTSSTS
Sbjct: 1   MADADGASSIPNSIPSDPSDANLQKKHASVIERLAIRNQTRLENSITRRSESDSSTSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGL NADSMLTNKQQQ
Sbjct: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLGNADSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY+ARDSPGIRDKDGEILVKNFKGSD+GEFTISGLSSCEVKLIG+VRALFIHKLRNCKV
Sbjct: 181 ASYNARDSPGIRDKDGEILVKNFKGSDIGEFTISGLSSCEVKLIGTVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDV+ECTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPYRVS
Sbjct: 241 YTGPVMGSILIDDVQECTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYRVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDLTDA+LGVETGNWENVDDF WLRAVPSPNWSILPEDERIDTIKIS TEG
Sbjct: 301 YEGIEEDLTDASLGVETGNWENVDDFLWLRAVPSPNWSILPEDERIDTIKISLTEG 356

BLAST of CSPI04G04490 vs. NCBI nr
Match: XP_023553493.1 (tubulin-folding cofactor C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 321/356 (90.17%), Postives = 335/356 (94.10%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MAD DG SSIPNSIP +PSD++LQKKHASVIERLANRNQTRLENSITRRSESDSS+SSTS
Sbjct: 1   MADDDGASSIPNSIPPHPSDSSLQKKHASVIERLANRNQTRLENSITRRSESDSSSSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIES LAQCRLTPP+P+Q+RSHLDGIS SISDLEKLVAE+SYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESDLAQCRLTPPEPSQVRSHLDGISASISDLEKLVAETSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQ+L++LNSELLPKKKFSFKNK TKKD KSESKD GL NA SMLTNKQQQ
Sbjct: 121 RASLKSISELKQSLEDLNSELLPKKKFSFKNKGTKKDTKSESKDLGLGNAGSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
           ASY ARDSPGIRDKDGE+LVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV
Sbjct: 181 ASYIARDSPGIRDKDGEVLVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVE CTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPY VS
Sbjct: 241 YTGPVMGSILIDDVEGCTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYCVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDL DA+LG ETGNWENVDDFRWLRAV SPNWSIL  DERIDT+KIS TEG
Sbjct: 301 YEGIEEDLKDASLGEETGNWENVDDFRWLRAVHSPNWSILAADERIDTVKISSTEG 356

BLAST of CSPI04G04490 vs. NCBI nr
Match: KAG6577293.1 (Tubulin-folding cofactor C, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.4 bits (1565), Expect = 7.7e-170
Identity = 320/356 (89.89%), Postives = 334/356 (93.82%), Query Frame = 0

Query: 1   MADADGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTS 60
           MAD DG SSIPNSIP +PSD++LQKKHASVIERLANRNQTRLENSITRRSESDSS+SSTS
Sbjct: 1   MADDDGASSIPNSIPPHPSDSSLQKKHASVIERLANRNQTRLENSITRRSESDSSSSSTS 60

Query: 61  SFLDRFSDSKRAIESALAQCRLTPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEV 120
           SFL RFSDSKRAIES LAQCRLTPP+P+Q+RSHLDGIS SISDLEKLVAE+SYSLPSYEV
Sbjct: 61  SFLVRFSDSKRAIESDLAQCRLTPPEPSQVRSHLDGISASISDLEKLVAETSYSLPSYEV 120

Query: 121 RASLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQ 180
           RASLKSISELKQ+L++LNSELLPKKKFSFKNK TKKD KSESKD GL NA SMLTNKQQQ
Sbjct: 121 RASLKSISELKQSLEDLNSELLPKKKFSFKNKGTKKDTKSESKDLGLGNAGSMLTNKQQQ 180

Query: 181 ASYSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240
            SY ARDSPGIRDKDGE+LVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV
Sbjct: 181 PSYIARDSPGIRDKDGEVLVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKV 240

Query: 241 YTGPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVS 300
           YTGPVMGSILIDDVE CTFAMASHQIRIHNAK SDFYLRVRSRPIIEDSSSVRFAPY VS
Sbjct: 241 YTGPVMGSILIDDVEGCTFAMASHQIRIHNAKNSDFYLRVRSRPIIEDSSSVRFAPYCVS 300

Query: 301 YEGIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKISPTEG 357
           YEGIEEDL DA+LG ETGNWENVDDFRWLRAV SPNWSIL E ERIDT+KIS TEG
Sbjct: 301 YEGIEEDLKDASLGEETGNWENVDDFRWLRAVQSPNWSILAEGERIDTVKISSTEG 356

BLAST of CSPI04G04490 vs. TAIR 10
Match: AT4G39920.1 (C-CAP/cofactor C-like domain-containing protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.4e-105
Identity = 208/350 (59.43%), Postives = 263/350 (75.14%), Query Frame = 0

Query: 5   DGPSSIPNSIPSYPSDANLQKKHASVIERLANRNQTRLENSITRRSESDSSTSSTSSFLD 64
           DG SS+  ++     D  LQKKH  ++ERL+ R+Q R  +S    S S S+  STSSFL 
Sbjct: 4   DGQSSVAETL-----DPALQKKHHDMLERLSARHQARKSDSPDSSSSSSSTLESTSSFLA 63

Query: 65  RFSDSKRAIESALAQCRL--TPPDPAQLRSHLDGISTSISDLEKLVAESSYSLPSYEVRA 124
           +FSDSKR+IES +A+ RL  +  D ++L+S L  IS +I +LEKL+AE+SY LPSYEVR+
Sbjct: 64  KFSDSKRSIESRIAESRLASSSTDSSKLKSDLAEISVAIDNLEKLLAENSYFLPSYEVRS 123

Query: 125 SLKSISELKQALDNLNSELLPKKKFSFKNKATKKDQKSESKDPGLENADSMLTNKQQQAS 184
           SLK +S+LKQ+LD L+ EL+PKKKFSFK+K+T K  K ESK P ++  D +L  K     
Sbjct: 124 SLKIVSDLKQSLDILSGELVPKKKFSFKSKSTTK--KPESKLPEIQKPDVVLPPK----L 183

Query: 185 YSARDSPGIRDKDGEILVKNFKGSDVGEFTISGLSSCEVKLIGSVRALFIHKLRNCKVYT 244
              RDSPG+R+K GE LVK+F+GS +GEFT+S L SC+VKL G+V ALF+H+L+ C VYT
Sbjct: 184 VPVRDSPGLRNKHGETLVKSFEGSSIGEFTLSDLDSCQVKLTGTVNALFLHRLKKCSVYT 243

Query: 245 GPVMGSILIDDVEECTFAMASHQIRIHNAKKSDFYLRVRSRPIIEDSSSVRFAPYRVSYE 304
           GPV+GSILIDDVE+C   +ASHQIRIH A+KSDFYLRVRSRPIIEDS+ VRFAPY + Y+
Sbjct: 244 GPVIGSILIDDVEDCVLVLASHQIRIHCARKSDFYLRVRSRPIIEDSNGVRFAPYCLDYK 303

Query: 305 GIEEDLTDATLGVETGNWENVDDFRWLRAVPSPNWSILPEDERIDTIKIS 353
           GI+EDL  A L  ET NW NVDDFRWLRAV SPNWS+LPE+ER+  + IS
Sbjct: 304 GIDEDLKTAGLEEETNNWANVDDFRWLRAVQSPNWSLLPEEERVSLLTIS 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMR27.6e-10459.43Tubulin-folding cofactor C OS=Arabidopsis thaliana OX=3702 GN=TFCC PE=1 SV=1[more]
Q8VCN95.0e-3932.39Tubulin-specific chaperone C OS=Mus musculus OX=10090 GN=Tbcc PE=1 SV=1[more]
Q5R5J71.2e-3730.72Tubulin-specific chaperone C OS=Pongo abelii OX=9601 GN=TBCC PE=2 SV=1[more]
Q158142.8e-3731.16Tubulin-specific chaperone C OS=Homo sapiens OX=9606 GN=TBCC PE=1 SV=2[more]
Q3SZE92.3e-3630.06Tubulin-specific chaperone C OS=Bos taurus OX=9913 GN=TBCC PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZA35.5e-19099.16Tubulin-folding cofactor C OS=Cucumis sativus OX=3659 GN=Csa_4G026830 PE=3 SV=1[more]
A0A5D3D2T65.9e-18496.63Tubulin-folding cofactor C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BX625.9e-18496.63Tubulin-folding cofactor C OS=Cucumis melo OX=3656 GN=LOC103494429 PE=3 SV=1[more]
A0A5A7TP941.3e-18396.35Tubulin-folding cofactor C OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A6J1EMN13.7e-17089.89Tubulin-folding cofactor C OS=Cucurbita moschata OX=3662 GN=LOC111435895 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_004146878.11.1e-18999.16tubulin-folding cofactor C [Cucumis sativus] >XP_031745695.1 tubulin-folding cof... [more]
XP_008453815.11.2e-18396.63PREDICTED: tubulin-folding cofactor C [Cucumis melo] >TYK16879.1 tubulin-folding... [more]
KAA0044708.12.7e-18396.35tubulin-folding cofactor C [Cucumis melo var. makuwa][more]
XP_023553493.12.0e-17090.17tubulin-folding cofactor C [Cucurbita pepo subsp. pepo][more]
KAG6577293.17.7e-17089.89Tubulin-folding cofactor C, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G39920.15.4e-10559.43C-CAP/cofactor C-like domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 120..140
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..190
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
IPR006599CARP motifSMARTSM00673carpcoord: 254..288
e-value: 2.2E-4
score: 30.6
coord: 216..253
e-value: 3.8E-6
score: 36.4
IPR038397TBCC, N-terminal domain superfamilyGENE3D1.20.58.1250coord: 24..145
e-value: 1.2E-23
score: 85.4
IPR031925Tubulin-specific chaperone C, N-terminalPFAMPF16752TBCC_Ncoord: 32..145
e-value: 5.5E-30
score: 104.1
IPR016098Cyclase-associated protein CAP/septum formation inhibitor MinC, C-terminalGENE3D2.160.20.70coord: 190..355
e-value: 1.5E-55
score: 189.4
IPR012945Tubulin binding cofactor C-like domainPFAMPF07986TBCCcoord: 210..326
e-value: 3.4E-39
score: 133.3
IPR027684Tubulin-specific chaperone CPANTHERPTHR15139TUBULIN FOLDING COFACTOR Ccoord: 23..352
IPR017901C-CAP/cofactor C-like domainPROSITEPS51329C_CAP_COFACTOR_Ccoord: 165..328
score: 34.252888

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G04490.1CSPI04G04490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000902 cell morphogenesis
biological_process GO:0007023 post-chaperonin tubulin folding pathway
biological_process GO:0007021 tubulin complex assembly
cellular_component GO:0005737 cytoplasm
molecular_function GO:0015631 tubulin binding