CSPI04G04410 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G04410
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Phospholipid scramblase
Location	Chr4: 2867174 .. 2872784 (+)
RNA-Seq Expression	CSPI04G04410
Synteny	CSPI04G04410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTTTTCTTTCTTCCCTTTTTCATTTGTTTTTCGTACGGCACATTCACTCAAGTTCTCCCTCTCTAGTCCCCCCGTCAAAATGAAGCTCGGTATTCAGCTCATCTTCAAAATTATAGAAACTTTCAACCATTCTAATGCATACAGCAAATTTTGATTTCTCTTTTTCACAATACAATTCAAGTTTCATTCTGCTCTAGATTTTGTGTATGCAATCCTAGATGAACTGGACTAGGAGTTCGCGTTTTTGGTCAAACACTGTTCGCAAATGCATCGGAGAAGATCGTTGTGCTAGAACCTCGCTTTGGCCAGTGCACAGGATTGCGCCTGGGTATCGTCATTTCGGAAGAAGGATTCAGAATGATGATAGTCCGCTCATAGATAGGAGCTTCCTGGCGAAGCTATGGGAAGCAGATAGGAAGCTGGAAGGTAGTAGAGAGAAACGGAAACGACATCGGATTGGTAATTATGATCGAAATGGAACTCAGAATCGGATTGGTCAGTATGATCGCACGCCGTTTGGGAAACCCACATTGGATGCTACTTTTGTAGAAGGGAAACCGTGGAAGCAACCACCTCCAAGTCAATCTGTCTCTGGTTTTCTAGAGCCACATTCTCCCGAAGAGGTTAGCTTAGATTTTAAGTTACATGCTTGTGCTTTGAGTCTTTGCTTTGTTATTTTCCGTTCATAACAATGAAACAGCAATATCTGTTATCGTTCCTCATTAGTTGAATCTTCCTCATTAGTTTTAATTTCATCACTAATTTTTCATTGGCATTCCTGTTTGGCTCATTTCATTATATGAATGATGTAGTTTGTTTGTTTTGTTGTATCTTCTGATGTCCTGTACTGTAATTACTCTAGGTTTAGTTGTCTTGCTAAACAGCTTTGTTTCGGTGTAATCTCGTTTTCAGTTGAAGGCTGTTCTATTTCTCGCTCTGAAAATGAGTGAAGAGCTAGACATTTGAGTTTTAGTCAAGTAAATCATGATTCATTGCTCTGCTGTATACTCTCTTGAACTTCGCTTCTTTGTGCTAAGACTAAGAACAATACTTCTTTCCGCATTGAGATTCCAGTTGAGGGAGTGGATTAGTTGAGATTTAGTATTAATATTGTCCACCATGGCTTATGGTAAAAAACAATATTACACCTTGAATCAAGATGACGTCAAATTTCTGATGAGCAGAGTACAATTAGTATTGGGGTTCCAAGCATGACAATCGCTTATGCTTACCTCATGACCCATAATCGCTTGTGTTCTTATCTTCGTGTGAATTTTATAGTAAAATGCTTGCTTCTTCTGCCATATTCCCACTGAATTACACTGACATGAGTGTTTCCTTCATATCTCCATACTTTAGGTCCGAGTTGCACCTCTTCTTGCTAGATCTAATTTACTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTTTTGGGTTTGAGCAGGTAACTCTTTTCCATCACTTGTTTTCTCCTGGTTAGTTATTCATGTGCAGATTTCTATGTCACGGTGTTTACAGTGTCCACCTATTTAGTATACCTTGAAAATTATGAAGGATTAGAATGACACAATTAAGGCCCTTATCAACCAAACTCCTAGCATCATCTTAAGAATGCAAAGAGTGCTTATGTAATTTGATAGATCCTTTTATTGAATTGTGTTTCTAGCCTTTTAACTTTCCTTTTTTTTAGGAAAAACCATGTCGATGATCTAACACACTTTTTTCATTTAATGGTATATTGGCAACCCCTCCCCCTTCGTCTTTTGCTGCTAGGTTCTAATTGGTTTATTTATACATTCTATCTACACGTTTTGAGCAATTGTGGTGGAATGTTATGGATCAAATTATGTTTATGGAAGTGTTAAGAACTTGACTGCTTCTTGTGATGTTTGAAGAAAGAAACCTTCAATAAGTAGTCACTAACACTTTAATAGCGTTACAAATCTTTTGCTTGTTTATCATCAAATTCTTGCTCGTATTCCTGTTTCTTTCATTTTAAATCCTAGGTTATGCTATATCACCACTTTAATTTGAGAAATGCAGGAAAATCGTTATGCTATAGTTGACGTCTGCTATCCCCAGTCAGTAAGTATCCATTTTCGTCTTTTAGTCACTCTTATTGTTTTACTTTTCCCTACTTTTATGTTTACCATATTCTTCTTTGACTTTACATACTGCAATTTTGTGTAATAGCCAGTTGGGTTAATTCGGGAGCAAAGTAGCATCATTGCTAGACAGGTATAATATAACTTAAAACCTTTTACATTACAATGACCAACTTAACTACAGTAACTATGACAATTCTGATGTGAAAATATTAGACTAAAACTTCAAATCCTGGTTGATTCATTTTCTTGCAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACGGATGCAATGGGTAATGAACTTTTCAGGGTAAGCATTTTAATGTTTTCCTTTTCCTTTTCAAGCAACAATCAAATATGACTTTCAGATTAATGTATACTCTTAGGTTCGGCGGCCCTTTTGGTGGATAACCAGTTCTATATATGCTGAAGTTGATGGTAAGGTATGATGGTTCAGTTATTTTTTTAGTGCATTCACAAAGAAACTCTTTTACCTTGTGGCACTCTGGACATGTTTTCACATTAGAAAAACAACTCTAGGAAATTGGTGTGGTCCATAGACGGTGGCATCTTTGGAGGAGGGTGTATGATCTGTACCTTGGGTAAGTTTAGCACAAACTATCTATATTGATTCATATTATTGCATCAAACTGATGCTGATTGAAACCTTGTCTAGGCATGCACGTGTTTGTTGAGTAATTATTCGAGGAAGTTGGCACAATGTAGTTATAGTTTGACTGTACATCCAAAGGTTTAGAAGGGGGGCCACCACGGTTTGGCAGTCATTGCATAATTGTTTGTGTTTGAGGGTTAAATTTCTATTGTTCCTGTTATGGTGTTCTAGGGTCATAATACATCCTTTTGCTCATCTATCTATTGGAGACAGTAAGAGGCTGTTTGGGTATATATTCTAAAAATTGTTATGATATAACCTTTTAAAACTGTTTTGGATTGTTAATAAGTTTTAGAAAAAGTTTGTGAAGATTTATTTGTTATTGTTGTTTCATTGATTTTTTCCCTATGGTAATTTTAAATACAACAAATTCGAATCAAATTATACTGAAAAATAACTATGCCAGTTCAACAAAACACAAGAATAATTTACTTTTGAGTAGGTTTTATAATTGAAATAATATACATGATATCAGTGTGTCATCCTTGATGCACTATTTGCTTTTCTCTATCATGTTTATGGTTATCTTATTTGCATTTAACTATACGAAGTCATGATGGAATCAACTGCCAATTTTGTATGAAACAGTTTTAGTTGTGACTTGTCAAACTTAAATTGTAACTTTCAGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGATTTTGGAGTTGGACCTTTACATTAAAGGACATTGATGGAAAAGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTTGGCTTTGAGGTCATTTCTTTTCCCTTCATGCAATAGCTTTAGGATAAAAACCAGTATTTCATACCAACGAGCATTTTCTGTTTAGTGAAATGTAAATTGTGGAAATGAAGAGATTTTCATAATCTTCTTCCCTCAGCTTCTCAGTAGCAGTGCTTCCAAATCTGCCATGACCCTGAGCCTTTTGCCATTCTCTCTTATTTTAATAGGATATTCTTCAAGGAACCTCTACTCTAGCACCTACACAAGCCGTACAAAAGAAAAAAAGGGGGATTGGAGTCTAAAGGATAATTACATGGAAAAAAATTCTAGTTAAAGATTCCCCAAGAGACACATTGATTCTGGCAATGGACCATATCTCACACGAGCTGTCAAACTCATATGAAATTCTTCTATTTATTTTTAACCAGATTGTTATCATGAAAAAAGGTATGAAAGAGTACCCTTCCATCATGTAGGACTCTCTGCTTCGTCCAATCTCTCACCAAAGGTCTTCAAAAATGCCTAAATCAAATTAACAAAAGGACAACTCCAAGGAATATGATCCAAGTCCGTTGCCACCCCCTAACAAAAGTTGTTCCACTACAGCCTAACACGATAGTTGATAGGGAAGAAGTAAGAATTCAATTCATTAGGACTCAATTAGATCGAGAATATAAATCAAGACTTGAGTGCCTCATGGATCTCCAAAATCAAGAATTTTTTCCCCTCAATACAATTAAATATTTAGCCTTTGATTGTTTCTGGATTTCATATCGAAGTTTTTTGAAATACAGTCAAATACTTGTGGGTCTGCCATCGCTTGTCTATATAACTGTCCAACTTGCCGGGATATTGATTTTTTGTGGAATCTGGTTATCAAGTCGTTACCTTTTTTCATATATGATTAATTTTTTGTCGTTGCCTTAATTTTATGTCATGGGCGCCAAAACTCACGCCGACGTGATTTGCATCTTCAGTTCTTCTTGTCTACATGTTATCATTTCTTGTAAACTTTTTTACTCAATTATCTCTTTATAGTCGATGAAATTAGTTCCACAGGGTATATATTTATTGGTTTTTGTTGTCTAAACTATGATGCAGATCTTTACTGATGCCGGTCAGTATGTCATTCGTTTTGGTGCTTCTGACCCGGTCTCAAGGACTGGTGCTGCTAGAGAAGTAATATTATTTGATTGATGTTAACTTGATTTTAGTTTTCATATAAATTACTTTTTCAATATTGTGTGCTGTTCATGGTGATAGGTTCAAGAGCTGGAAGTCGCCCGGCACCTTACTCTTTCAGAAAGAGCCGTAGCTGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGTGGCTGGTAAGTGGTAAACACTTCTTCATAAAGCCTGCACTCAAAGATCATAAGTTGCATCATATGTCTCGCTAGATTTCTACTCCATAAAATTGTAACATCATCGTGTCTTGTTTTGTTTGCCCCAGTGAAAGTCCGTAACATATAATTATCTCAAATCTTTCATTTTCTTTCTGCATTCTTGAGTTCTAAGATTGACAATGGTGATTAGTTTGTTCATTTTTTATTTTCAATGTCTTGTGGGGTGGCATAAGAAATTATTATAGGAGATTACCACATTGTGGGCAGTAGTTTTCCAGAAAAACGACAACGTATTTCAAATTTGTATCTTATAACTAATAAAGGGCTTTCAAATTCCAACTATATATTTAAGGTTGAGGATGGAGTCATCATAGTTTTAACTCAATAATTTTCCATCAAAGCAAACACTAATCATTGATTTACATATTCTTCATCAGGGGACTTCCTTTCGTTGCCGTTGGTGAGTAGATTTGGTTTGCTGGACAGACTTCTCGATATCTGGGGTTTCTTAGGTGTTACTGTACACCACATCTTATTATCTTGTGCTTTTCATGTTGATAGAGGATTATTTTCCTCCGAAGTTCCACTTATGTAGGGCATGATATTTAAACCTTGACCTCTTGGTCGAAGATACTCTTAAGCTCAATTGACCTATGCCCATCTTGTCATGTTTTTTATACAAATTCTATCAATACTAATGAAATCCAAATTGT

mRNA sequence

CTTTTTTTCTTTCTTCCCTTTTTCATTTGTTTTTCGTACGGCACATTCACTCAAGTTCTCCCTCTCTAGTCCCCCCGTCAAAATGAAGCTCGGTATTCAGCTCATCTTCAAAATTATAGAAACTTTCAACCATTCTAATGCATACAGCAAATTTTGATTTCTCTTTTTCACAATACAATTCAAGTTTCATTCTGCTCTAGATTTTGTGTATGCAATCCTAGATGAACTGGACTAGGAGTTCGCGTTTTTGGTCAAACACTGTTCGCAAATGCATCGGAGAAGATCGTTGTGCTAGAACCTCGCTTTGGCCAGTGCACAGGATTGCGCCTGGGTATCGTCATTTCGGAAGAAGGATTCAGAATGATGATAGTCCGCTCATAGATAGGAGCTTCCTGGCGAAGCTATGGGAAGCAGATAGGAAGCTGGAAGGTAGTAGAGAGAAACGGAAACGACATCGGATTGGTAATTATGATCGAAATGGAACTCAGAATCGGATTGGTCAGTATGATCGCACGCCGTTTGGGAAACCCACATTGGATGCTACTTTTGTAGAAGGGAAACCGTGGAAGCAACCACCTCCAAGTCAATCTGTCTCTGGTTTTCTAGAGCCACATTCTCCCGAAGAGGTCCGAGTTGCACCTCTTCTTGCTAGATCTAATTTACTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTTTTGGGTTTGAGCAGGAAAATCGTTATGCTATAGTTGACGTCTGCTATCCCCAGTCACCAGTTGGGTTAATTCGGGAGCAAAGTAGCATCATTGCTAGACAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACGGATGCAATGGGTAATGAACTTTTCAGGGTTCGGCGGCCCTTTTGGTGGATAACCAGTTCTATATATGCTGAAGTTGATGGTAAGGAAATTGGTGTGGTCCATAGACGGTGGCATCTTTGGAGGAGGGTGTATGATCTGTACCTTGGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGATTTTGGAGTTGGACCTTTACATTAAAGGACATTGATGGAAAAGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTTGGCTTTGAGATCTTTACTGATGCCGGTCAGTATGTCATTCGTTTTGGTGCTTCTGACCCGGTCTCAAGGACTGGTGCTGCTAGAGAAGTTCAAGAGCTGGAAGTCGCCCGGCACCTTACTCTTTCAGAAAGAGCCGTAGCTGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGTGGCTGGGGACTTCCTTTCGTTGCCGTTGGTGAGTAGATTTGGTTTGCTGGACAGACTTCTCGATATCTGGGGTTTCTTAGGTGTTACTGTACACCACATCTTATTATCTTGTGCTTTTCATGTTGATAGAGGATTATTTTCCTCCGAAGTTCCACTTATGTAGGGCATGATATTTAAACCTTGACCTCTTGGTCGAAGATACTCTTAAGCTCAATTGACCTATGCCCATCTTGTCATGTTTTTTATACAAATTCTATCAATACTAATGAAATCCAAATTGT

Coding sequence (CDS)

ATGAACTGGACTAGGAGTTCGCGTTTTTGGTCAAACACTGTTCGCAAATGCATCGGAGAAGATCGTTGTGCTAGAACCTCGCTTTGGCCAGTGCACAGGATTGCGCCTGGGTATCGTCATTTCGGAAGAAGGATTCAGAATGATGATAGTCCGCTCATAGATAGGAGCTTCCTGGCGAAGCTATGGGAAGCAGATAGGAAGCTGGAAGGTAGTAGAGAGAAACGGAAACGACATCGGATTGGTAATTATGATCGAAATGGAACTCAGAATCGGATTGGTCAGTATGATCGCACGCCGTTTGGGAAACCCACATTGGATGCTACTTTTGTAGAAGGGAAACCGTGGAAGCAACCACCTCCAAGTCAATCTGTCTCTGGTTTTCTAGAGCCACATTCTCCCGAAGAGGTCCGAGTTGCACCTCTTCTTGCTAGATCTAATTTACTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTTTTGGGTTTGAGCAGGAAAATCGTTATGCTATAGTTGACGTCTGCTATCCCCAGTCACCAGTTGGGTTAATTCGGGAGCAAAGTAGCATCATTGCTAGACAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACGGATGCAATGGGTAATGAACTTTTCAGGGTTCGGCGGCCCTTTTGGTGGATAACCAGTTCTATATATGCTGAAGTTGATGGTAAGGAAATTGGTGTGGTCCATAGACGGTGGCATCTTTGGAGGAGGGTGTATGATCTGTACCTTGGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGATTTTGGAGTTGGACCTTTACATTAAAGGACATTGATGGAAAAGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTTGGCTTTGAGATCTTTACTGATGCCGGTCAGTATGTCATTCGTTTTGGTGCTTCTGACCCGGTCTCAAGGACTGGTGCTGCTAGAGAAGTTCAAGAGCTGGAAGTCGCCCGGCACCTTACTCTTTCAGAAAGAGCCGTAGCTGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGTGGCTGGGGACTTCCTTTCGTTGCCGTTGGTGAGTAG

Protein sequence

MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAKLWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDAGQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLPFVAVGE*

Homology

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BLAST of CSPI04G04410 vs. ExPASy Swiss-Prot
Match: P47140 (Altered inheritance rate of mitochondria protein 25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AIM25 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.7e-37
Identity = 104/298 (34.90%), Postives = 151/298 (50.67%), Query Frame = 0


Query: 99  PFGKPTLDATFVEGKPWKQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANL 158
           PF +   +   +   P  +     S SG ++ H P       +L    ++I R IE+ N+
Sbjct: 36  PFRRVIREKNQITKAPSVKAFKENSNSGIIKVHDP---IATTILNEPTVIIERQIEFMNV 95

Query: 159 VFGFEQENRYAIVDVCYPQSPVGLIREQS--SIIARQLLRLRRPFVAYITDAMGNELFRV 218
             GFEQ NRYAI+DV   +    + R+ S    I RQ  RL RPF+  + D  GN +  +
Sbjct: 96  FLGFEQANRYAIMDVNGNKIASMMERDFSITKAIMRQFYRLHRPFLVDVFDNWGNVIMTI 155

Query: 219 RRPFWWITSSIYAEV----------------DGKE---IGVVHRRWHLWRRVYDLYLGN- 278
           +RPF +I S I   +                DGKE   +G   + WHLWRR Y+L+  + 
Sbjct: 156 KRPFSFINSHIKTIIPPSAYVDNGSDSTHYHDGKEGTTVGETIQNWHLWRRRYELFQKDG 215

Query: 279 ------KQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDAGQYVIRFGAS 338
                  QF  ++ P F S+ F + D DGK++A VDR+W G G E+FTD G YV+RF + 
Sbjct: 216 VEGSTFDQFGKIDAP-FLSFDFPVTDADGKIMASVDRNWVGLGREMFTDTGVYVVRFDSQ 275

Query: 339 ---DPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLPFVAVG 366
              D +  T       E+  ++ LTL +RAV +A A+S+D DYFSRH      F++ G
Sbjct: 276 RCFDNIYPT-------EMLSSQVLTLDQRAVLLANAVSIDFDYFSRHSRQTGGFLSFG 322

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BLAST of CSPI04G04410 vs. ExPASy Swiss-Prot
Match: Q9UT84 (Phospholipid scramblase family protein C343.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC343.06c PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-34
Identity = 96/277 (34.66%), Postives = 147/277 (53.07%), Query Frame = 0


Query: 105 LDATFVEGKPWKQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQ 164
           +DAT    K   Q P + S +G +  +SP     APLL++  L++ R +E  N+  G+EQ
Sbjct: 44  IDAT---AKVVSQEPAAISSTGAIPLNSP----AAPLLSQDVLIVERQLEMMNVFLGYEQ 103

Query: 165 ENRYAIVDVCYPQSPVGLIREQS-----SIIARQLLRLRRPFVAYITDAMGNELFRVRRP 224
            NRY I++       +G I EQ      S ++RQ     R F A + D+ G  + ++ RP
Sbjct: 104 ANRYVILN--QQGQHLGYIAEQGASSILSSLSRQFFHTHRAFKADVMDSNGQLVLQLNRP 163

Query: 225 FWWITSSI------YAEVDGKEIGVVHRRWHLWRRVYDLYLGNK----QFAVVENPGFWS 284
           F WI S +      Y++     +G V ++WHLWRR Y+L+L  +    QFA ++     S
Sbjct: 164 FSWINSRLQIHSIDYSKFSSTLVGEVLQKWHLWRRRYELFLAKRSMFEQFAKIDE-RVLS 223

Query: 285 WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDAGQYVIRFGASDPVSRTGAAREVQELEV-- 344
           W F L++   ++L  V R++ G   E FTD G YV+RF ++   +  G+  E Q L+   
Sbjct: 224 WEFLLRNEQDRILGSVSRNFMGLPREFFTDTGNYVLRFTSTS--AANGSVNENQLLQAAH 283

Query: 345 -------ARHLTLSERAVAVALAISLDNDYFSR-HGG 357
                  AR ++L ERAV +  A+++D DYFSR HGG
Sbjct: 284 GIANDVCARDMSLEERAVMLGSAVTIDFDYFSRIHGG 308

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BLAST of CSPI04G04410 vs. ExPASy Swiss-Prot
Match: Q9NRQ2 (Phospholipid scramblase 4 OS=Homo sapiens OX=9606 GN=PLSCR4 PE=1 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-10
Identity = 62/219 (28.31%), Postives = 90/219 (41.10%), Query Frame = 0


Query: 110 VEGKPWKQPPPSQSVSGFLEPHSPEEVRVAP----LLARSNLLITRDIEWANLVFGFEQE 169
           V  +P K P P+QSV     P         P    L+   N+ + +  E   ++  FE  
Sbjct: 81  VRYQPGKYPMPNQSVPITWMPGPTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETN 140

Query: 170 NRYAIVDVCYPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPF----- 229
           NRY I +       V ++ E +    R   R  RPFV  +TD MG E+  ++RPF     
Sbjct: 141 NRYDIKN--NSDQMVYIVTEDTDDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCC 200

Query: 230 WWITSSIYAEVD-----GKEIGVVHRRWHLWRRVYDLYLGNKQFAV-VENP----GFWS- 289
            +   S   E++     G  IG V   W+L R VY +    K+  + V  P    G  S 
Sbjct: 201 CFCCPSARQELEVQCPPGVTIGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSD 260

Query: 290 WTFTLKDIDG-KVLAEVDRDWRGFGFEIFTDAGQYVIRF 308
             F +K +DG   +  + R W G       DA  + I F
Sbjct: 261 SVFEVKSLDGISNIGSIIRKWNGL-LSAMADADHFDIHF 296

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BLAST of CSPI04G04410 vs. ExPASy Swiss-Prot
Match: P58196 (Phospholipid scramblase 4 OS=Mus musculus OX=10090 GN=Plscr4 PE=2 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 61/232 (26.29%), Postives = 95/232 (40.95%), Query Frame = 0


Query: 99  PFGKPTLDATFVEGKPWK-----QPPPSQSVSGFLE-PHSPEEVRVAPLLARSNLLITRD 158
           P   PT     ++ +P K     QP P   ++G    P+ P  +    L    N+ + + 
Sbjct: 66  PLYHPTGGTHPIQYQPGKYPVTNQPAPIMWMAGPAPVPNCPPGLEY--LAQLDNIHVLQH 125

Query: 159 IEWANLVFGFEQENRYAIVDVCYPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNE 218
           +E   L+  FE  NRY I +       V ++ E +    R   R  RPFV  +TD +G E
Sbjct: 126 VEPLELMTRFETNNRYDIKNNI--DQMVYIVTEDTDDFTRNAYRNLRPFVLRVTDCLGRE 185

Query: 219 LFRVRRPF-----WWITSSIYAEVD-----GKEIGVVHRRWHLWRRVYDLYLGNKQFAV- 278
           +  ++RPF      +       E++     G  IG V   W+L R  Y +    K+  + 
Sbjct: 186 IMTMQRPFRCTCCCFCCPCARQELEVQCPPGVTIGFVAEHWNLCRASYSIQNEKKESMMR 245

Query: 279 VENP----GFWS-WTFTLKDIDG-KVLAEVDRDWRGFGFEIFTDAGQYVIRF 308
           V  P    G  S   F +  +DG   +  + R W GF      +A  + IRF
Sbjct: 246 VRGPCATYGCGSDSVFEINSLDGVSNIGSIIRKWNGF-LSTMVNADHFEIRF 292

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BLAST of CSPI04G04410 vs. ExPASy TrEMBL
Match: A0A0A0KXQ8 (Phospholipid scramblase OS=Cucumis sativus OX=3659 GN=Csa_4G026250 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 2.9e-218
Identity = 365/366 (99.73%), Postives = 366/366 (100.00%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQ+DDSPLIDRSFLAK
Sbjct: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQSDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH
Sbjct: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360

Query: 361 FVAVGE 367
           FVAVGE
Sbjct: 361 FVAVGE 366

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BLAST of CSPI04G04410 vs. ExPASy TrEMBL
Match: A0A5A7TN09 (Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004590 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.3e-207
Identity = 348/366 (95.08%), Postives = 352/366 (96.17%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRS RFWSNTVRKCI ED C RTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK
Sbjct: 53  MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 112

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNG QNRIG YDR  FGK TLDATF EGKPWKQPPP
Sbjct: 113 LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYDRRAFGKSTLDATFAEGKPWKQPPP 172

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEV+VAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 173 SQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 232

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           G IREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAEVDGKEIGVVH
Sbjct: 233 GFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGKEIGVVH 292

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 293 RRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 352

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTG AR VQELEVAR LTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 353 GQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRHGGWGLP 412

Query: 361 FVAVGE 367
           F+AVGE
Sbjct: 413 FIAVGE 418

[Close]

BLAST of CSPI04G04410 vs. ExPASy TrEMBL
Match: A0A5D3CZC9 (Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00160 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.3e-207
Identity = 348/366 (95.08%), Postives = 352/366 (96.17%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRS RFWSNTVRKCI ED C RTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK
Sbjct: 1   MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNG QNRIG YDR  FGK TLDATF EGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYDRRAFGKSTLDATFAEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEV+VAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           G IREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAEVDGKEIGVVH
Sbjct: 181 GFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTG AR VQELEVAR LTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 301 GQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRHGGWGLP 360

Query: 361 FVAVGE 367
           F+AVGE
Sbjct: 361 FIAVGE 366

[Close]

BLAST of CSPI04G04410 vs. ExPASy TrEMBL
Match: A0A1S3BXA7 (Phospholipid scramblase OS=Cucumis melo OX=3656 GN=LOC103494434 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.3e-207
Identity = 348/366 (95.08%), Postives = 352/366 (96.17%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRS RFWSNTVRKCI ED C RTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK
Sbjct: 1   MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNG QNRIG YDR  FGK TLDATF EGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYDRRAFGKSTLDATFAEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEV+VAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           G IREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAEVDGKEIGVVH
Sbjct: 181 GFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTG AR VQELEVAR LTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 301 GQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRHGGWGLP 360

Query: 361 FVAVGE 367
           F+AVGE
Sbjct: 361 FIAVGE 366

[Close]

BLAST of CSPI04G04410 vs. ExPASy TrEMBL
Match: A0A6J1FQC4 (Phospholipid scramblase OS=Cucurbita moschata OX=3662 GN=LOC111447826 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 9.4e-185
Identity = 315/372 (84.68%), Postives = 330/372 (88.71%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRSSRFWSNT RKCIGEDRC R+SLWPV RIAP YRHFG RI NDD PL+DRSFLA+
Sbjct: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYD------RTPFGKPTLDATFVEGKP 120
           LW+ DR L  SREKRKR       RNG QNRI  Y+        PF   T DATFVEGK 
Sbjct: 61  LWDVDRNLGSSREKRKR----LLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQ 120

Query: 121 WKQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVC 180
           WKQPPPSQSVSGFL PH+PEEV+VAPLLARSNLLITRDIEWANLV GFEQENRYAIVDVC
Sbjct: 121 WKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVC 180

Query: 181 YPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGK 240
           YPQSPVG IREQS+I+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAE+DGK
Sbjct: 181 YPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGK 240

Query: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGF 300
           EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDG VLAEVDRDWRGFGF
Sbjct: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGF 300

Query: 301 EIFTDAGQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360
           E+FTDAGQYVIRFGASD  SRTG A+ VQELEVARHLTLSERAVAVALAISLDNDYFSRH
Sbjct: 301 EMFTDAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360

Query: 361 GGWGLPFVAVGE 367
           GGWGLPF+AVGE
Sbjct: 361 GGWGLPFIAVGE 368

[Close]

BLAST of CSPI04G04410 vs. NCBI nr
Match: XP_004146884.1 (altered inheritance rate of mitochondria protein 25 isoform X1 [Cucumis sativus] >KGN53197.1 hypothetical protein Csa_014924 [Cucumis sativus])

HSP 1 Score: 767.3 bits (1980), Expect = 6.0e-218
Identity = 365/366 (99.73%), Postives = 366/366 (100.00%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQ+DDSPLIDRSFLAK
Sbjct: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQSDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH
Sbjct: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360

Query: 361 FVAVGE 367
           FVAVGE
Sbjct: 361 FVAVGE 366

[Close]

BLAST of CSPI04G04410 vs. NCBI nr
Match: XP_031740685.1 (altered inheritance rate of mitochondria protein 25 isoform X2 [Cucumis sativus])

HSP 1 Score: 748.8 bits (1932), Expect = 2.2e-212
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQ+DDSPLIDRSFLAK
Sbjct: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQSDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH
Sbjct: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGW 358
           GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGW
Sbjct: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGW 357

[Close]

BLAST of CSPI04G04410 vs. NCBI nr
Match: KAA0044702.1 (altered inheritance rate of mitochondria protein 25-like [Cucumis melo var. makuwa])

HSP 1 Score: 731.1 bits (1886), Expect = 4.7e-207
Identity = 348/366 (95.08%), Postives = 352/366 (96.17%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRS RFWSNTVRKCI ED C RTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK
Sbjct: 53  MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 112

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNG QNRIG YDR  FGK TLDATF EGKPWKQPPP
Sbjct: 113 LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYDRRAFGKSTLDATFAEGKPWKQPPP 172

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEV+VAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 173 SQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 232

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           G IREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAEVDGKEIGVVH
Sbjct: 233 GFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGKEIGVVH 292

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 293 RRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 352

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTG AR VQELEVAR LTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 353 GQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRHGGWGLP 412

Query: 361 FVAVGE 367
           F+AVGE
Sbjct: 413 FIAVGE 418

[Close]

BLAST of CSPI04G04410 vs. NCBI nr
Match: XP_008453824.1 (PREDICTED: altered inheritance rate of mitochondria protein 25-like [Cucumis melo] >TYK16885.1 altered inheritance rate of mitochondria protein 25-like [Cucumis melo var. makuwa])

HSP 1 Score: 731.1 bits (1886), Expect = 4.7e-207
Identity = 348/366 (95.08%), Postives = 352/366 (96.17%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRS RFWSNTVRKCI ED C RTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK
Sbjct: 1   MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFVEGKPWKQPPP 120
           LWEADRKLEGSREKRKRHRIGNYDRNG QNRIG YDR  FGK TLDATF EGKPWKQPPP
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYDRRAFGKSTLDATFAEGKPWKQPPP 120

Query: 121 SQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180
           SQSVSGFLEPHSPEEV+VAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV
Sbjct: 121 SQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVCYPQSPV 180

Query: 181 GLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGVVH 240
           G IREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAEVDGKEIGVVH
Sbjct: 181 GFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGKEIGVVH 240

Query: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300
           RRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA
Sbjct: 241 RRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGFEIFTDA 300

Query: 301 GQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLP 360
           GQYVIRFGASDPVSRTG AR VQELEVAR LTLSERAVAVALAISLDNDYFSRHGGWGLP
Sbjct: 301 GQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRHGGWGLP 360

Query: 361 FVAVGE 367
           F+AVGE
Sbjct: 361 FIAVGE 366

[Close]

BLAST of CSPI04G04410 vs. NCBI nr
Match: XP_022942941.1 (altered inheritance rate of mitochondria protein 25 [Cucurbita moschata])

HSP 1 Score: 656.0 bits (1691), Expect = 1.9e-184
Identity = 315/372 (84.68%), Postives = 330/372 (88.71%), Query Frame = 0


Query: 1   MNWTRSSRFWSNTVRKCIGEDRCARTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60
           MNWTRSSRFWSNT RKCIGEDRC R+SLWPV RIAP YRHFG RI NDD PL+DRSFLA+
Sbjct: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYD------RTPFGKPTLDATFVEGKP 120
           LW+ DR L  SREKRKR       RNG QNRI  Y+        PF   T DATFVEGK 
Sbjct: 61  LWDVDRNLGSSREKRKR----LLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQ 120

Query: 121 WKQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVC 180
           WKQPPPSQSVSGFL PH+PEEV+VAPLLARSNLLITRDIEWANLV GFEQENRYAIVDVC
Sbjct: 121 WKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVC 180

Query: 181 YPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGK 240
           YPQSPVG IREQS+I+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAE+DGK
Sbjct: 181 YPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGK 240

Query: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDWRGFGF 300
           EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFW+WTFTLKDIDG VLAEVDRDWRGFGF
Sbjct: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGF 300

Query: 301 EIFTDAGQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360
           E+FTDAGQYVIRFGASD  SRTG A+ VQELEVARHLTLSERAVAVALAISLDNDYFSRH
Sbjct: 301 EMFTDAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360

Query: 361 GGWGLPFVAVGE 367
           GGWGLPF+AVGE
Sbjct: 361 GGWGLPFIAVGE 368

[Close]

BLAST of CSPI04G04410 vs. TAIR 10
Match: AT2G04940.1 (scramblase-related )

HSP 1 Score: 454.5 bits (1168), Expect = 8.0e-128
Identity = 218/317 (68.77%), Postives = 258/317 (81.39%), Query Frame = 0


Query: 51  PLIDRSFLAKLWEADRKLEGSREKRKRHRIGNYDRNGTQNRIGQYDRTPFGKPTLDATFV 110
           P +DR FLA+LW AD+K   + E  KR++  +  RN T+N               D  F 
Sbjct: 90  PSLDRKFLAQLWVADKKKLKAME--KRYKKASKHRNYTEN------------DGFDVHFE 149

Query: 111 EGKPW-KQPPPSQSVSGFLEPHSPEEVRVAPLLARSNLLITRDIEWANLVFGFEQENRYA 170
             +P  +QPP SQS+SG L+P + +E ++A LLARSNLL+TRDIEWANLV GFEQENRY 
Sbjct: 150 TVEPTVEQPPVSQSMSGLLKPKTSDEAKIATLLARSNLLVTRDIEWANLVLGFEQENRYI 209

Query: 171 IVDVCYPQSPVGLIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYA 230
           +VDVCYP++PVG IREQS+++ARQLLR RRPFVA ITDA+GNELFRVRRPFWW+TSSIYA
Sbjct: 210 VVDVCYPEAPVGSIREQSNLLARQLLRTRRPFVASITDALGNELFRVRRPFWWLTSSIYA 269

Query: 231 EVDGKEIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWSWTFTLKDIDGKVLAEVDRDW 290
           E+DG+EIGVVHRRWHLWRR+YDLYLGN QFAVVENPGFW+WTFT+KD DG+VLA++DRDW
Sbjct: 270 EIDGEEIGVVHRRWHLWRRIYDLYLGNNQFAVVENPGFWNWTFTVKDADGEVLAQIDRDW 329

Query: 291 RGFGFEIFTDAGQYVIRFGASDPVSRTGAAREVQELEVARHLTLSERAVAVALAISLDND 350
           RGFGFEIFTDAGQYVIRFG +D  ++TG A  V+ELEV R LTLSERAV + LAISLDND
Sbjct: 330 RGFGFEIFTDAGQYVIRFGKADAAAKTGPATLVEELEVKRPLTLSERAVVLTLAISLDND 389

Query: 351 YFSRHGGWGLPFVAVGE 367
           YFSRHGGWG+PF+AVGE
Sbjct: 390 YFSRHGGWGIPFMAVGE 392

The following BLAST results are available for this feature:

BLAST of CSPI04G04410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P47140	3.7e-37	34.90	Altered inheritance rate of mitochondria protein 25 OS=Saccharomyces cerevisiae ...	[more]
Q9UT84	1.3e-34	34.66	Phospholipid scramblase family protein C343.06c OS=Schizosaccharomyces pombe (st...	[more]
Q9NRQ2	2.3e-10	28.31	Phospholipid scramblase 4 OS=Homo sapiens OX=9606 GN=PLSCR4 PE=1 SV=2	[more]
P58196	1.0e-07	26.29	Phospholipid scramblase 4 OS=Mus musculus OX=10090 GN=Plscr4 PE=2 SV=2	[more]

BLAST of CSPI04G04410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXQ8	2.9e-218	99.73	Phospholipid scramblase OS=Cucumis sativus OX=3659 GN=Csa_4G026250 PE=3 SV=1	[more]
A0A5A7TN09	2.3e-207	95.08	Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3CZC9	2.3e-207	95.08	Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BXA7	2.3e-207	95.08	Phospholipid scramblase OS=Cucumis melo OX=3656 GN=LOC103494434 PE=3 SV=1	[more]
A0A6J1FQC4	9.4e-185	84.68	Phospholipid scramblase OS=Cucurbita moschata OX=3662 GN=LOC111447826 PE=3 SV=1	[more]

BLAST of CSPI04G04410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146884.1	6.0e-218	99.73	altered inheritance rate of mitochondria protein 25 isoform X1 [Cucumis sativus]...	[more]
XP_031740685.1	2.2e-212	99.72	altered inheritance rate of mitochondria protein 25 isoform X2 [Cucumis sativus]	[more]
KAA0044702.1	4.7e-207	95.08	altered inheritance rate of mitochondria protein 25-like [Cucumis melo var. maku...	[more]
XP_008453824.1	4.7e-207	95.08	PREDICTED: altered inheritance rate of mitochondria protein 25-like [Cucumis mel...	[more]
XP_022942941.1	1.9e-184	84.68	altered inheritance rate of mitochondria protein 25 [Cucurbita moschata]	[more]

BLAST of CSPI04G04410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04940.1	8.0e-128	68.77	scramblase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005552	Scramblase	PFAM	PF03803	Scramblase	coord: 127..353 e-value: 1.4E-64 score: 217.4
IPR005552	Scramblase	PANTHER	PTHR23248	PHOSPHOLIPID SCRAMBLASE-RELATED	coord: 113..360
None	No IPR available	PANTHER	PTHR23248:SF9	PHOSPHOLIPID SCRAMBLASE	coord: 113..360
IPR025659	Tubby-like, C-terminal	SUPERFAMILY	54518	Tubby C-terminal domain-like	coord: 204..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G04410.1	CSPI04G04410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017121	plasma membrane phospholipid scrambling
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0017128	phospholipid scramblase activity