CSPI04G04300 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G04300
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Tyrosine--tRNA ligase
Location	Chr4: 2807185 .. 2808972 (-)
RNA-Seq Expression	CSPI04G04300
Synteny	CSPI04G04300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAATTTCTTCATTTTGACGATTTTTCATTTCTGGTGTGGGGATTGAATTCACATTTTTGGATTAGCCATAGCCAAAGAGCCTCTTTCTGATAGAAAGAACTGAACCGAATTGAATCCATGGTGATGGCCATTTTTGCTTCTTCTTCATCTTCTTGTTACACCTCTAAAGCTTTCCTTCTCTCTCAACGTTCCTTCTTTTTCTCCACTATTTCATCCAAACCCTCTTCCTCCTTCTTCTTCAAACCCACTTCCCGTTTCTTCTCTTCTTCCTCTCCAACTTCTCTTCACTACTCACCAACCCCTTCTCCTCCCACCAACGTCATTCAAATCCTCGAACAACGCGGCCTTCTCGACTCCATCACCAGCGACAATCTCCGCTCCGCTTCTCTCTCCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACTGCCCAGAGCTTACATTTAGGTAATCTTCTTGGTTTAATCGTCCTCTCTTGGTTCCGCCGATGTGGCCACTCCACCGTCGCTCTTATCGGCGGCGCCACTGCCCGTGTCGGAGACCCCTCCGGCAAGAGCCTCGAGCGGCCCGAACTCGACCTCCAAACCTTGGAGGCTAATACTCTCGGAATTACTAATATTATCACTAAAATTTTGGGGAATTCGGGGTCGGATTCTGATTTTTGCCCTAATTTTGTGATTCTGAATAATTATGATTGGTGGAAGGAGTTTAGGTTTTTGGATTTCTTGAAAGATGTGGGTCGGTTTGCGAGAGTGGGATCCATGATTGCTAAGGAGAGTGTTAGGAGGAGATTGGAATCGGAGCAAGGAATGAGTTATACTGAGTTCACTTATCAGCTTTTGCAAGGGTATGATTTTCTTCATTTGTATCAAAACGAGGGTGTTTCTGTTCAGATTGGAGGTAGTGATCAATGGGGGAATATTACTGCTGGGACAGAGCTTATTAGGAAACGTTTGCCTCAAGCTGATGGCGTTTATGGTTTGACATTCCCTCTTCTTCTGAAGAGTGATGGCACCAAATTTGGTAAATCCGAAGACGGTGCTATTTGGCTTTCGCCGTCGATGTTATCTCCGTACAAGTTTTATCAGTATTTCTTCAGTGTTCCTGATGCTGATGTGGTGAGGTTTCTTAGAATTCTCACATTTTTGGATATTGAGGAGATTCAGGACTTGGAAAGCCAAATGGTGAAACCTGGGTATGTTCCTAACACTGCACAGAGGCGGCTGGCCGAAGAAGTTACTCGGTTTGTTCATGGTGAAGATGGTTTACGTGAGGCCCTTAAGGCTACTGAAGCATTGAAACCAGGAGCAGAGACAAAGTTGGATTGGAAGACTATTGAAGGGATTGCTGAGGATGTTCCATGCTGCTCGTTGGAGTATGATCAAGTGTTGAACCTTTCGGTTGTCGATCTCTCAGTTACGTCTGGTTTGCTTGAAAGCAAGTCGGCTGCCCGTCGGTTACTGAAGCAAGGGGGACTTTATTTGAATAATGGTAGAGTGGATTCCGAGAGTAAAAGGGTTGAAGCTGTAGACATTGTTGATGGAAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATAACACAGAGCGATTCAGAAACAGCTCATTCATTCCCTAACCAACAAGTCCTTCATTTTTACTATTACTTTTTGGGTGAAATTCTTTGGTAAACTATTTCAAGTTCATAGTTAAAGTTCCAATCTTCAAATTGATGTTTATTACCAATTTACCTGTTTTAGAGTAGTTTAGAAACGGTTAGACCACTTTTG

mRNA sequence

CCAATTTCTTCATTTTGACGATTTTTCATTTCTGGTGTGGGGATTGAATTCACATTTTTGGATTAGCCATAGCCAAAGAGCCTCTTTCTGATAGAAAGAACTGAACCGAATTGAATCCATGGTGATGGCCATTTTTGCTTCTTCTTCATCTTCTTGTTACACCTCTAAAGCTTTCCTTCTCTCTCAACGTTCCTTCTTTTTCTCCACTATTTCATCCAAACCCTCTTCCTCCTTCTTCTTCAAACCCACTTCCCGTTTCTTCTCTTCTTCCTCTCCAACTTCTCTTCACTACTCACCAACCCCTTCTCCTCCCACCAACGTCATTCAAATCCTCGAACAACGCGGCCTTCTCGACTCCATCACCAGCGACAATCTCCGCTCCGCTTCTCTCTCCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACTGCCCAGAGCTTACATTTAGGTAATCTTCTTGGTTTAATCGTCCTCTCTTGGTTCCGCCGATGTGGCCACTCCACCGTCGCTCTTATCGGCGGCGCCACTGCCCGTGTCGGAGACCCCTCCGGCAAGAGCCTCGAGCGGCCCGAACTCGACCTCCAAACCTTGGAGGCTAATACTCTCGGAATTACTAATATTATCACTAAAATTTTGGGGAATTCGGGGTCGGATTCTGATTTTTGCCCTAATTTTGTGATTCTGAATAATTATGATTGGTGGAAGGAGTTTAGGTTTTTGGATTTCTTGAAAGATGTGGGTCGGTTTGCGAGAGTGGGATCCATGATTGCTAAGGAGAGTGTTAGGAGGAGATTGGAATCGGAGCAAGGAATGAGTTATACTGAGTTCACTTATCAGCTTTTGCAAGGGTATGATTTTCTTCATTTGTATCAAAACGAGGGTGTTTCTGTTCAGATTGGAGGTAGTGATCAATGGGGGAATATTACTGCTGGGACAGAGCTTATTAGGAAACGTTTGCCTCAAGCTGATGGCGTTTATGGTTTGACATTCCCTCTTCTTCTGAAGAGTGATGGCACCAAATTTGGTAAATCCGAAGACGGTGCTATTTGGCTTTCGCCGTCGATGTTATCTCCGTACAAGTTTTATCAGTATTTCTTCAGTGTTCCTGATGCTGATGTGGTGAGGTTTCTTAGAATTCTCACATTTTTGGATATTGAGGAGATTCAGGACTTGGAAAGCCAAATGGTGAAACCTGGGTATGTTCCTAACACTGCACAGAGGCGGCTGGCCGAAGAAGTTACTCGGTTTGTTCATGGTGAAGATGGTTTACGTGAGGCCCTTAAGGCTACTGAAGCATTGAAACCAGGAGCAGAGACAAAGTTGGATTGGAAGACTATTGAAGGGATTGCTGAGGATGTTCCATGCTGCTCGTTGGAGTATGATCAAGTGTTGAACCTTTCGGTTGTCGATCTCTCAGTTACGTCTGGTTTGCTTGAAAGCAAGTCGGCTGCCCGTCGGTTACTGAAGCAAGGGGGACTTTATTTGAATAATGGTAGAGTGGATTCCGAGAGTAAAAGGGTTGAAGCTGTAGACATTGTTGATGGAAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATAACACAGAGCGATTCAGAAACAGCTCATTCATTCCCTAACCAACAAGTCCTTCATTTTTACTATTACTTTTTGGGTGAAATTCTTTGGTAAACTATTTCAAGTTCATAGTTAAAGTTCCAATCTTCAAATTGATGTTTATTACCAATTTACCTGTTTTAGAGTAGTTTAGAAACGGTTAGACCACTTTTG

Coding sequence (CDS)

ATGGTGATGGCCATTTTTGCTTCTTCTTCATCTTCTTGTTACACCTCTAAAGCTTTCCTTCTCTCTCAACGTTCCTTCTTTTTCTCCACTATTTCATCCAAACCCTCTTCCTCCTTCTTCTTCAAACCCACTTCCCGTTTCTTCTCTTCTTCCTCTCCAACTTCTCTTCACTACTCACCAACCCCTTCTCCTCCCACCAACGTCATTCAAATCCTCGAACAACGCGGCCTTCTCGACTCCATCACCAGCGACAATCTCCGCTCCGCTTCTCTCTCCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACTGCCCAGAGCTTACATTTAGGTAATCTTCTTGGTTTAATCGTCCTCTCTTGGTTCCGCCGATGTGGCCACTCCACCGTCGCTCTTATCGGCGGCGCCACTGCCCGTGTCGGAGACCCCTCCGGCAAGAGCCTCGAGCGGCCCGAACTCGACCTCCAAACCTTGGAGGCTAATACTCTCGGAATTACTAATATTATCACTAAAATTTTGGGGAATTCGGGGTCGGATTCTGATTTTTGCCCTAATTTTGTGATTCTGAATAATTATGATTGGTGGAAGGAGTTTAGGTTTTTGGATTTCTTGAAAGATGTGGGTCGGTTTGCGAGAGTGGGATCCATGATTGCTAAGGAGAGTGTTAGGAGGAGATTGGAATCGGAGCAAGGAATGAGTTATACTGAGTTCACTTATCAGCTTTTGCAAGGGTATGATTTTCTTCATTTGTATCAAAACGAGGGTGTTTCTGTTCAGATTGGAGGTAGTGATCAATGGGGGAATATTACTGCTGGGACAGAGCTTATTAGGAAACGTTTGCCTCAAGCTGATGGCGTTTATGGTTTGACATTCCCTCTTCTTCTGAAGAGTGATGGCACCAAATTTGGTAAATCCGAAGACGGTGCTATTTGGCTTTCGCCGTCGATGTTATCTCCGTACAAGTTTTATCAGTATTTCTTCAGTGTTCCTGATGCTGATGTGGTGAGGTTTCTTAGAATTCTCACATTTTTGGATATTGAGGAGATTCAGGACTTGGAAAGCCAAATGGTGAAACCTGGGTATGTTCCTAACACTGCACAGAGGCGGCTGGCCGAAGAAGTTACTCGGTTTGTTCATGGTGAAGATGGTTTACGTGAGGCCCTTAAGGCTACTGAAGCATTGAAACCAGGAGCAGAGACAAAGTTGGATTGGAAGACTATTGAAGGGATTGCTGAGGATGTTCCATGCTGCTCGTTGGAGTATGATCAAGTGTTGAACCTTTCGGTTGTCGATCTCTCAGTTACGTCTGGTTTGCTTGAAAGCAAGTCGGCTGCCCGTCGGTTACTGAAGCAAGGGGGACTTTATTTGAATAATGGTAGAGTGGATTCCGAGAGTAAAAGGGTTGAAGCTGTAGACATTGTTGATGGAAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATAA

Protein sequence

MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSPTPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS*

Homology

[Close]

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot
Match: Q9M876 (Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=EMB2768 PE=2 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 5.8e-187
Identity = 345/506 (68.18%), Postives = 399/506 (78.85%), Query Frame = 0


Query: 6   FASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYS------ 65
           FAS S     S+ FL   R         K S++FF +       S+S T+L  S      
Sbjct: 9   FASRSILPICSRTFLSPLRVASLLVFPEKSSATFFRRVQVPHLFSTSTTTLFSSVKCSIH 68

Query: 66  PTPSPPT--------NVIQILEQRGLLDSITSDNLRSA----SLSPLKVYCGFDPTAQSL 125
            T SP T        NV+ ILE+RGLL+SITS+NLRSA     ++PL+VYCGFDPTA+SL
Sbjct: 69  STSSPETENQAVFRPNVVDILEERGLLESITSENLRSACSDPKVAPLRVYCGFDPTAESL 128

Query: 126 HLGNLLGLIVLSWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNI 185
           HLGNLLG+IVLSWF+RCGH  V LIGGAT RVGDPSGKSLERPELD  TLE N  GIT I
Sbjct: 129 HLGNLLGIIVLSWFQRCGHQAVGLIGGATGRVGDPSGKSLERPELDADTLEKNIAGITKI 188

Query: 186 ITKILGNSGSDSDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLES 245
           I KILG++ S      ++VI NNYDWWK+   LDFL  VGRFARVG+M+AKESV++RLES
Sbjct: 189 IIKILGSNPSPGG---SYVIFNNYDWWKDMTMLDFLNKVGRFARVGTMMAKESVKKRLES 248

Query: 246 EQGMSYTEFTYQLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYG 305
           EQGMSYTEFTYQLLQ YDFLHL++NEG++VQIGGSDQWGNITAGT+LIRK L   +  YG
Sbjct: 249 EQGMSYTEFTYQLLQAYDFLHLFKNEGINVQIGGSDQWGNITAGTDLIRKILQAEEAAYG 308

Query: 306 LTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEE 365
           LTFPLLLK+DGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPD DV+RFL+ LTFL ++E
Sbjct: 309 LTFPLLLKNDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDVDVIRFLKTLTFLSLDE 368

Query: 366 IQDLESQMVKPGYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIE 425
           I+ LE QM KPGYVPNTAQ +LAEEVTRFVHGE+GL+EA+KATEAL+PGAETKLDW  IE
Sbjct: 369 IKILEDQMSKPGYVPNTAQIKLAEEVTRFVHGEEGLKEAIKATEALRPGAETKLDWNLIE 428

Query: 426 GIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRV 485
            IAED+P CSL  D+V  LS+VDLSV++GL ESKSAARR+LKQGG Y+NN RVD E+KRV
Sbjct: 429 RIAEDIPSCSLPIDRVSGLSIVDLSVSAGLFESKSAARRMLKQGGFYMNNERVDDENKRV 488

Query: 486 EAVDIVDGKVLLLSAGKKNKVVVRIS 494
           +  DIV+G+ L+LSAGKKNKVVVRIS
Sbjct: 489 DEEDIVEGRGLVLSAGKKNKVVVRIS 511

[Close]

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot
Match: Q6MC06 (Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=tyrS PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.4e-132
Identity = 224/427 (52.46%), Postives = 315/427 (73.77%), Query Frame = 0


Query: 66  TNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCG 125
           TNV+ +L +RG +D++TS+ +R  +  P+KVYCGFDPTA SLHLGNL+ ++ L+WF+R G
Sbjct: 2   TNVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRFG 61

Query: 126 HSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNF 185
           H+ VA++GGAT  +GDPSGK+ ER  LD + ++ N  GI+  +  IL  +   +      
Sbjct: 62  HTPVAIVGGATGMIGDPSGKNAERQLLDEKKIQLNLKGISKNLEAILDFNHPTAP----A 121

Query: 186 VILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGYD 245
           +ILNN DW+K F F+ FL+DVG+  R+  M+AK+SV+ RL SE+GMS+TEF YQ+LQGYD
Sbjct: 122 IILNNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSEEGMSFTEFCYQILQGYD 181

Query: 246 FLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSE 305
           FLHL++N GV+V++GGSDQWGNITAGT+LIRK    A   YG+TFPLL KSDG KFGKSE
Sbjct: 182 FLHLFENYGVTVELGGSDQWGNITAGTDLIRK--VHAKPAYGITFPLLTKSDGQKFGKSE 241

Query: 306 DGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNTA 365
            GA+WLSP  LS Y+FYQ+   V DADV+  +R+LTFLD+ EI+  E  M +  YVP TA
Sbjct: 242 KGAVWLSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTA 301

Query: 366 QRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLN 425
           Q+RLAEE+TR VHGE+GL+ A+K TE + PG++T L+   +E +A D+P C ++ + VLN
Sbjct: 302 QKRLAEEITRLVHGEEGLKIAIKVTEGVAPGSQTSLNADILEKLAADMPSCEMKLENVLN 361

Query: 426 LSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKK 485
             ++DL V + L  SKS ARRLL+ GG+Y+NN +++ E+  +    ++  +++LL+AGKK
Sbjct: 362 KKLIDLLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVECLISSRLILLAAGKK 421

Query: 486 NKVVVRI 493
           NK+++R+
Sbjct: 422 NKMIIRL 422

[Close]

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot
Match: A5UUZ9 (Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.9e-93
Identity = 184/429 (42.89%), Postives = 269/429 (62.70%), Query Frame = 0


Query: 66  TNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCG 125
           T ++  L  RGLL   +       +   + +Y GFDP+A SLH+G+LL L+ L+ F+R G
Sbjct: 2   TTILDELRWRGLLYESSDGLDELLTRERVTLYIGFDPSADSLHIGHLLPLLTLARFQRWG 61

Query: 126 HSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNF 185
           H+ +AL GG T  +GDPSGK+ ERP L  + + AN   I   + + L  +  D     + 
Sbjct: 62  HTPIALAGGGTGLIGDPSGKTQERPLLSKEQVAANVEAIKGQLAQFLDFNAGDH----SA 121

Query: 186 VILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGYD 245
           ++LNN DW      +DFL+DVG+   V  M+AK+SVR R+ SE G+S+TEF+Y LLQ YD
Sbjct: 122 LLLNNADWLTRLSLMDFLRDVGKHLTVNYMLAKDSVRSRMTSETGISFTEFSYMLLQAYD 181

Query: 246 FLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSE 305
           F +L+++ G  +Q GGSDQWGNITAG ELIR+ L Q    YGL +PL+ K+DGTKFGK+E
Sbjct: 182 FAYLFEHYGCKLQAGGSDQWGNITAGIELIRRTLGQK--AYGLVYPLVTKADGTKFGKTE 241

Query: 306 DGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNTA 365
            GA+WL P   SPY+FYQ++++V DADV ++LR  T+L  +EI++LE ++        TA
Sbjct: 242 SGAVWLDPKRTSPYRFYQFWYNVDDADVGKYLRYFTWLSADEIEELE-RITASQPERRTA 301

Query: 366 QRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVL- 425
           Q+RLA EVTR VHGE  L  A +A++AL  G+  +L    +  I EDVP  +L       
Sbjct: 302 QQRLAREVTRMVHGETALARAEEASQALFGGSLARLSAGDVIDIFEDVPSTTLPRQDFEG 361

Query: 426 -NLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAG 485
               ++D+ +  G+  SKS ARR ++ GG+Y+NN +     +R+   D +DG+ ++L  G
Sbjct: 362 DGFPLIDVLLRCGIATSKSDARRTIEGGGIYVNNIQCPDVGRRLTLNDSIDGQFIVLRKG 421

Query: 486 KKNKVVVRI 493
           +K   +V++
Sbjct: 422 RKQYHLVKV 423

[Close]

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot
Match: A7NK64 (Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=383372 GN=tyrS PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 6.4e-93
Identity = 187/430 (43.49%), Postives = 271/430 (63.02%), Query Frame = 0


Query: 66  TNVIQILEQRGLLDSITSDNLRS-ASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRC 125
           T ++  L  RGLL   +SD L        + +Y GFDP+A SLH+G+LL L+ L+ F+R 
Sbjct: 2   TTILDELRWRGLLYE-SSDGLDDLLERERVTLYIGFDPSADSLHIGHLLPLLTLARFQRW 61

Query: 126 GHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPN 185
           GH+ +AL GG T  +GDPSGK+ ERP L  + + AN   I   + + L  +  D     +
Sbjct: 62  GHTPIALAGGGTGLIGDPSGKTQERPLLSKEQVAANVEAIKRQLAQFLDFNAGDH----S 121

Query: 186 FVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGY 245
            ++LNN +W      +DFL+DVG+   V  M+AK+SVR R+ SE G+S+TEF+Y LLQ Y
Sbjct: 122 AMLLNNAEWLTRLSLMDFLRDVGKHLTVNYMLAKDSVRSRMMSETGISFTEFSYMLLQAY 181

Query: 246 DFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKS 305
           DF +L+++ G  +Q GGSDQWGNITAG ELIR+   Q    YGL +PL+ K+DGTKFGK+
Sbjct: 182 DFAYLFEHYGCKLQAGGSDQWGNITAGIELIRRTSGQK--AYGLVYPLVTKADGTKFGKT 241

Query: 306 EDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNT 365
           E GA+WL P   SPY+FYQ++++V DADV ++LR  T+L   EI++LE Q+        T
Sbjct: 242 ESGAVWLDPKRTSPYRFYQFWYNVDDADVGKYLRYFTWLSAAEIEELE-QITARQPERRT 301

Query: 366 AQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVL 425
           AQ+RLA EVTR VHGE  L  A +A++AL  G+   L    +  I EDVP  +L    + 
Sbjct: 302 AQQRLAREVTRMVHGEAALARAEEASQALFGGSLASLSADDVADIFEDVPSITLPRQDLE 361

Query: 426 --NLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSA 485
              +S++D  V  G+  SKS ARR ++ GG+Y+NN +     +R+   D +DG+ ++L  
Sbjct: 362 GDGMSLIDALVRCGIATSKSDARRAIEGGGIYINNVQSADVGRRLTLHDSIDGQCIVLRK 421

Query: 486 GKKNKVVVRI 493
           G+++  ++R+
Sbjct: 422 GRRHYHLIRL 423

[Close]

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot
Match: Q8A2S5 (Tyrosine--tRNA ligase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=tyrS PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.9e-92
Identity = 187/428 (43.69%), Postives = 266/428 (62.15%), Query Frame = 0


Query: 67  NVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCGH 126
           N ++ L  RG+L  I        +   +  Y G DPTA SLH+G+L G+++L   +RCGH
Sbjct: 2   NFVEELRWRGMLQDIMPGTEELLNKEQVTAYLGIDPTADSLHIGHLCGVMILRHLQRCGH 61

Query: 127 STVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNFV 186
             +ALIGGAT  +GDPSGKS ER  L+ +TL  N   I   + K L     +SD      
Sbjct: 62  KPLALIGGATGMIGDPSGKSAERNLLNEETLRHNQACIKKQLAKFL---DFESDVPNRAE 121

Query: 187 ILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRL--ESEQGMSYTEFTYQLLQGY 246
           ++NNYDW KEF FLDF+++VG+   V  M+AK+SV+RRL  E+  G+S+TEFTYQLLQGY
Sbjct: 122 LVNNYDWMKEFSFLDFVREVGKHITVNYMMAKDSVKRRLNGEARDGLSFTEFTYQLLQGY 181

Query: 247 DFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKS 306
           DFLHLY+ +G  +Q+GGSDQWGNIT G ELIR+       V+ LT PL+ K+DG KFGK+
Sbjct: 182 DFLHLYETKGCKLQMGGSDQWGNITTGAELIRR--TNGGEVFALTCPLITKADGGKFGKT 241

Query: 307 EDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNT 366
           E G IWL P   SPYKFYQ++ +V D+D  R+++I T ++ EEI+ L ++  +  ++   
Sbjct: 242 ESGNIWLDPRYTSPYKFYQFWLNVSDSDAERYIKIFTSIEKEEIEALVAEHQQAPHL-RA 301

Query: 367 AQRRLAEEVTRFVHGEDGLREALKATEALKPGAET----KLDWKTIEGIAEDVPCCSLEY 426
            Q+RLA+EVT  VH E+    A+ A+  L   A +    KLD  T+  + E VP   +  
Sbjct: 302 LQKRLAKEVTIMVHSEEDYNAAVDASNILFGNATSESLRKLDEDTLLAVFEGVPQFEISR 361

Query: 427 DQVL-NLSVVDLSV-TSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVL 486
           D +   +  VDL V  + +  SK   R+L++ GG+ LN  ++++  + +   D++DGK L
Sbjct: 362 DALAEGVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLEAFDQVITTADLLDGKYL 421

[Close]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL
Match: A0A0A0KU70 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.8e-277
Identity = 492/493 (99.80%), Postives = 493/493 (100.00%), Query Frame = 0


Query: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60
           MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP
Sbjct: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60

Query: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120
           TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW
Sbjct: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120

Query: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180
           FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD
Sbjct: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180

Query: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240
           FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL
Sbjct: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240

Query: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300
           LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK
Sbjct: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300

Query: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGY 360
           FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQM+KPGY
Sbjct: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMMKPGY 360

Query: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420
           VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY
Sbjct: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420

Query: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480
           DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL
Sbjct: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480

Query: 481 SAGKKNKVVVRIS 494
           SAGKKNKVVVRIS
Sbjct: 481 SAGKKNKVVVRIS 493

[Close]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL
Match: A0A5D3CZD9 (Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00290 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 6.7e-271
Identity = 479/493 (97.16%), Postives = 486/493 (98.58%), Query Frame = 0


Query: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60
           MVMAI ASSS SCYTSKAFLLSQRSFFFSTISSKPSSSFF KPTSRFF+SSSPTSL YSP
Sbjct: 1   MVMAILASSSPSCYTSKAFLLSQRSFFFSTISSKPSSSFFLKPTSRFFASSSPTSLRYSP 60

Query: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120
           TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW
Sbjct: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120

Query: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180
           FRRCGHSTVALIGGATAR+GDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD
Sbjct: 121 FRRCGHSTVALIGGATARIGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180

Query: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240
           FCPNF+ILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL
Sbjct: 181 FCPNFLILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240

Query: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300
           LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK
Sbjct: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300

Query: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGY 360
           FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQM KPGY
Sbjct: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGY 360

Query: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420
           VPNT QRRLAEEVTRFVHGE+GL EALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY
Sbjct: 361 VPNTVQRRLAEEVTRFVHGENGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420

Query: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480
           DQVLNLSVVDLSVT+GLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVD++DGKVLLL
Sbjct: 421 DQVLNLSVVDLSVTTGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLL 480

Query: 481 SAGKKNKVVVRIS 494
           SAGKKNKVVVRIS
Sbjct: 481 SAGKKNKVVVRIS 493

[Close]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL
Match: A0A1S4E026 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103494447 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 6.7e-271
Identity = 479/493 (97.16%), Postives = 486/493 (98.58%), Query Frame = 0


Query: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60
           MVMAI ASSS SCYTSKAFLLSQRSFFFSTISSKPSSSFF KPTSRFF+SSSPTSL YSP
Sbjct: 1   MVMAILASSSPSCYTSKAFLLSQRSFFFSTISSKPSSSFFLKPTSRFFASSSPTSLRYSP 60

Query: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120
           TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW
Sbjct: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120

Query: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180
           FRRCGHSTVALIGGATAR+GDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD
Sbjct: 121 FRRCGHSTVALIGGATARIGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180

Query: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240
           FCPNF+ILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL
Sbjct: 181 FCPNFLILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240

Query: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300
           LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK
Sbjct: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300

Query: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGY 360
           FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQM KPGY
Sbjct: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGY 360

Query: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420
           VPNT QRRLAEEVTRFVHGE+GL EALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY
Sbjct: 361 VPNTVQRRLAEEVTRFVHGENGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420

Query: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480
           DQVLNLSVVDLSVT+GLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVD++DGKVLLL
Sbjct: 421 DQVLNLSVVDLSVTTGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLL 480

Query: 481 SAGKKNKVVVRIS 494
           SAGKKNKVVVRIS
Sbjct: 481 SAGKKNKVVVRIS 493

[Close]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL
Match: A0A6J1FW55 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.1e-228
Identity = 412/488 (84.43%), Postives = 445/488 (91.19%), Query Frame = 0


Query: 10  SSSCYTSKAFLLSQRSFFFST--ISSKPSSSFFFKPTSRFFSSSSPT--SLHYSPTPSPP 69
           ++S  +S  FLLSQR+ FFS   ISSKPS SF  K T    +SS+ +  S    P  +  
Sbjct: 4   AASARSSMTFLLSQRTLFFSNSIISSKPSLSFSLKSTFSCLASSASSLQSSTLEPQTASR 63

Query: 70  TNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCG 129
            NV++ILE+RGLL+SITSDNLRSA LSPLKVYCGFDPTA+SLHLGNLLGLIVLSWFRRCG
Sbjct: 64  RNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCG 123

Query: 130 HSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNF 189
           H+TVALIGGATARVGDPSGKSLERPELDLQTLE NTLGITNIIT+ILGNSG DS+F  +F
Sbjct: 124 HTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNFSSDF 183

Query: 190 VILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGYD 249
           VILNNYDWWKEFRFLDFLK+VGRFARVG+M+AKESV+RRLES+QGMSYTEFTYQLLQGYD
Sbjct: 184 VILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLLQGYD 243

Query: 250 FLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSE 309
           FL+LYQN+GVSVQIGGSDQWGNITAGTELIRK LPQADGVYGLTFPLLLKSDGTKFGKS 
Sbjct: 244 FLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKFGKSV 303

Query: 310 DGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNTA 369
           DGAIWLSPS LSPYKFYQYFFSVPDADV+RFLRILTFLDIEEI+DLESQM  PGYVPNTA
Sbjct: 304 DGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYVPNTA 363

Query: 370 QRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLN 429
           QRRLAEEVTRFVHGEDGL EAL+ATEALKPGAETKLDWKTI GI EDVPCCSLEYDQVLN
Sbjct: 364 QRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYDQVLN 423

Query: 430 LSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKK 489
           LSVVDL+V SGLLESKSAARRLLKQGGLYLNN RVDSESK++EA+DI+DGKVLLLSAGKK
Sbjct: 424 LSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLSAGKK 483

Query: 490 NKVVVRIS 494
           NK+VVRIS
Sbjct: 484 NKMVVRIS 491

[Close]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL
Match: A0A6J1CIM7 (Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.4e-228
Identity = 420/492 (85.37%), Postives = 447/492 (90.85%), Query Frame = 0


Query: 7   ASSSSSCYTSKAFLLSQRSFFFST--ISSKPSSSFFFKPTSRFFSSSSPTSLHYS---PT 66
           A+ +S+C  S+ FLLSQRS FFST  ISSK + SF    +S    +SS +SL  S   P 
Sbjct: 2   AAIASAC-GSRTFLLSQRSLFFSTFNISSKSTVSFSSVRSSCRCIASSASSLQSSTLEPH 61

Query: 67  PSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWF 126
            +   NV++ILE+RGLL+SITSDNLRSASLSPLKVYCGFDPTA+SLHLGNLLGLIVLSWF
Sbjct: 62  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 121

Query: 127 RRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDF 186
           RRCGH+TVALIGGATARVGDPSGKSLERPELDL+TLEANTLGITN I +ILGNSGSDS+F
Sbjct: 122 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSIARILGNSGSDSNF 181

Query: 187 CPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLL 246
            PNFVILNNYDWWKEFRFLDFLK VGRFARVG+M+AKESVRRRLESEQGMSYTEFTYQLL
Sbjct: 182 SPNFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLESEQGMSYTEFTYQLL 241

Query: 247 QGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKF 306
           Q YDFLHL+QNEGVSVQIGGSDQWGNITAGTELIRK LP ADGVYGLTFPLLLKSDGTKF
Sbjct: 242 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 301

Query: 307 GKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYV 366
           GKSEDGA+WLSPSMLSPYKFYQYFFSVPDADV RFL+ILTFLD+++IQDLESQM   GYV
Sbjct: 302 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVARFLKILTFLDMKDIQDLESQMGSSGYV 361

Query: 367 PNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYD 426
           PNTAQRRLAEEVTRFVHGEDGL EALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YD
Sbjct: 362 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 421

Query: 427 QVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLS 486
           QVLNL +VDLSVTSGLLESKSAARRLLKQGGLYLNN RVDSE KRVEA DIVDGKVLLLS
Sbjct: 422 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 481

Query: 487 AGKKNKVVVRIS 494
           AGKKNKVVVRIS
Sbjct: 482 AGKKNKVVVRIS 492

[Close]

BLAST of CSPI04G04300 vs. NCBI nr
Match: XP_004146892.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN53185.1 hypothetical protein Csa_014838 [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 1.0e-276
Identity = 492/493 (99.80%), Postives = 493/493 (100.00%), Query Frame = 0


Query: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60
           MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP
Sbjct: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60

Query: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120
           TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW
Sbjct: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120

Query: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180
           FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD
Sbjct: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180

Query: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240
           FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL
Sbjct: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240

Query: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300
           LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK
Sbjct: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300

Query: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGY 360
           FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQM+KPGY
Sbjct: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMMKPGY 360

Query: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420
           VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY
Sbjct: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420

Query: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480
           DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL
Sbjct: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480

Query: 481 SAGKKNKVVVRIS 494
           SAGKKNKVVVRIS
Sbjct: 481 SAGKKNKVVVRIS 493

[Close]

BLAST of CSPI04G04300 vs. NCBI nr
Match: XP_016901583.1 (PREDICTED: tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KAA0044689.1 tyrosine--tRNA ligase [Cucumis melo var. makuwa] >TYK16895.1 tyrosine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 942.6 bits (2435), Expect = 1.4e-270
Identity = 479/493 (97.16%), Postives = 486/493 (98.58%), Query Frame = 0


Query: 1   MVMAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYSP 60
           MVMAI ASSS SCYTSKAFLLSQRSFFFSTISSKPSSSFF KPTSRFF+SSSPTSL YSP
Sbjct: 1   MVMAILASSSPSCYTSKAFLLSQRSFFFSTISSKPSSSFFLKPTSRFFASSSPTSLRYSP 60

Query: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120
           TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW
Sbjct: 61  TPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSW 120

Query: 121 FRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180
           FRRCGHSTVALIGGATAR+GDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD
Sbjct: 121 FRRCGHSTVALIGGATARIGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSD 180

Query: 181 FCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240
           FCPNF+ILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL
Sbjct: 181 FCPNFLILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQL 240

Query: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300
           LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK
Sbjct: 241 LQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTK 300

Query: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGY 360
           FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQM KPGY
Sbjct: 301 FGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGY 360

Query: 361 VPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420
           VPNT QRRLAEEVTRFVHGE+GL EALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY
Sbjct: 361 VPNTVQRRLAEEVTRFVHGENGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEY 420

Query: 421 DQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLL 480
           DQVLNLSVVDLSVT+GLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVD++DGKVLLL
Sbjct: 421 DQVLNLSVVDLSVTTGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLL 480

Query: 481 SAGKKNKVVVRIS 494
           SAGKKNKVVVRIS
Sbjct: 481 SAGKKNKVVVRIS 493

[Close]

BLAST of CSPI04G04300 vs. NCBI nr
Match: XP_038892591.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038892594.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038892599.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 891.7 bits (2303), Expect = 2.8e-255
Identity = 461/495 (93.13%), Postives = 471/495 (95.15%), Query Frame = 0


Query: 3   MAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLH----Y 62
           MAI ASSSS CY SKAFLLSQRSFFFSTI SKPS SFF K TSRFF+SSSPTSL     +
Sbjct: 1   MAILASSSSYCYGSKAFLLSQRSFFFSTIPSKPSFSFFLKTTSRFFASSSPTSLQSSSLH 60

Query: 63  SPTPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVL 122
           S TPS P NV+QILEQRGLLDSITSD LRSA LSPLKVYCGFDPTAQSLHLGNLLGLIVL
Sbjct: 61  SQTPSSP-NVLQILEQRGLLDSITSDTLRSACLSPLKVYCGFDPTAQSLHLGNLLGLIVL 120

Query: 123 SWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSD 182
           SWFRRCGH+ VALIGGATARVGDPSGKSLERPELDL TLEANTLGITNIIT+ILGNSGSD
Sbjct: 121 SWFRRCGHTAVALIGGATARVGDPSGKSLERPELDLHTLEANTLGITNIITRILGNSGSD 180

Query: 183 SDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTY 242
           S FCPNFVILNNYDWWKEFRFLDFLK+VGRFARVGSM+AKESV+RRLESEQGMSYTEFTY
Sbjct: 181 SSFCPNFVILNNYDWWKEFRFLDFLKEVGRFARVGSMMAKESVKRRLESEQGMSYTEFTY 240

Query: 243 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 302
           QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG
Sbjct: 241 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 300

Query: 303 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKP 362
           TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQD+ESQM KP
Sbjct: 301 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDMESQMAKP 360

Query: 363 GYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 422
           GY+PNTAQRRLAEEVTRFVHGEDGL EALKATEALKPGAETKLDWK IEGIAEDVPCCSL
Sbjct: 361 GYLPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKIIEGIAEDVPCCSL 420

Query: 423 EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVL 482
           EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNN RVDSESKRVEAVDIVDGKVL
Sbjct: 421 EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVL 480

Query: 483 LLSAGKKNKVVVRIS 494
           LLSAGKKNKVVVRIS
Sbjct: 481 LLSAGKKNKVVVRIS 494

[Close]

BLAST of CSPI04G04300 vs. NCBI nr
Match: XP_038892604.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892611.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892618.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892625.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-252
Identity = 455/490 (92.86%), Postives = 466/490 (95.10%), Query Frame = 0


Query: 3   MAIFASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLH----Y 62
           MAI ASSSS CY SKAFLLSQRSFFFSTI SKPS SFF K TSRFF+SSSPTSL     +
Sbjct: 1   MAILASSSSYCYGSKAFLLSQRSFFFSTIPSKPSFSFFLKTTSRFFASSSPTSLQSSSLH 60

Query: 63  SPTPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVL 122
           S TPS P NV+QILEQRGLLDSITSD LRSA LSPLKVYCGFDPTAQSLHLGNLLGLIVL
Sbjct: 61  SQTPSSP-NVLQILEQRGLLDSITSDTLRSACLSPLKVYCGFDPTAQSLHLGNLLGLIVL 120

Query: 123 SWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSD 182
           SWFRRCGH+ VALIGGATARVGDPSGKSLERPELDL TLEANTLGITNIIT+ILGNSGSD
Sbjct: 121 SWFRRCGHTAVALIGGATARVGDPSGKSLERPELDLHTLEANTLGITNIITRILGNSGSD 180

Query: 183 SDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTY 242
           S FCPNFVILNNYDWWKEFRFLDFLK+VGRFARVGSM+AKESV+RRLESEQGMSYTEFTY
Sbjct: 181 SSFCPNFVILNNYDWWKEFRFLDFLKEVGRFARVGSMMAKESVKRRLESEQGMSYTEFTY 240

Query: 243 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 302
           QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG
Sbjct: 241 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 300

Query: 303 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKP 362
           TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQD+ESQM KP
Sbjct: 301 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDMESQMAKP 360

Query: 363 GYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 422
           GY+PNTAQRRLAEEVTRFVHGEDGL EALKATEALKPGAETKLDWK IEGIAEDVPCCSL
Sbjct: 361 GYLPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKIIEGIAEDVPCCSL 420

Query: 423 EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVL 482
           EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNN RVDSESKRVEAVDIVDGKVL
Sbjct: 421 EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVL 480

Query: 483 LLSAGKKNKV 489
           LLSAGKKNK+
Sbjct: 481 LLSAGKKNKL 489

[Close]

BLAST of CSPI04G04300 vs. NCBI nr
Match: XP_022942360.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 802.4 bits (2071), Expect = 2.2e-228
Identity = 412/488 (84.43%), Postives = 445/488 (91.19%), Query Frame = 0


Query: 10  SSSCYTSKAFLLSQRSFFFST--ISSKPSSSFFFKPTSRFFSSSSPT--SLHYSPTPSPP 69
           ++S  +S  FLLSQR+ FFS   ISSKPS SF  K T    +SS+ +  S    P  +  
Sbjct: 4   AASARSSMTFLLSQRTLFFSNSIISSKPSLSFSLKSTFSCLASSASSLQSSTLEPQTASR 63

Query: 70  TNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVLSWFRRCG 129
            NV++ILE+RGLL+SITSDNLRSA LSPLKVYCGFDPTA+SLHLGNLLGLIVLSWFRRCG
Sbjct: 64  RNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCG 123

Query: 130 HSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSDSDFCPNF 189
           H+TVALIGGATARVGDPSGKSLERPELDLQTLE NTLGITNIIT+ILGNSG DS+F  +F
Sbjct: 124 HTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNFSSDF 183

Query: 190 VILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTYQLLQGYD 249
           VILNNYDWWKEFRFLDFLK+VGRFARVG+M+AKESV+RRLES+QGMSYTEFTYQLLQGYD
Sbjct: 184 VILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLLQGYD 243

Query: 250 FLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSE 309
           FL+LYQN+GVSVQIGGSDQWGNITAGTELIRK LPQADGVYGLTFPLLLKSDGTKFGKS 
Sbjct: 244 FLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKFGKSV 303

Query: 310 DGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMVKPGYVPNTA 369
           DGAIWLSPS LSPYKFYQYFFSVPDADV+RFLRILTFLDIEEI+DLESQM  PGYVPNTA
Sbjct: 304 DGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYVPNTA 363

Query: 370 QRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLN 429
           QRRLAEEVTRFVHGEDGL EAL+ATEALKPGAETKLDWKTI GI EDVPCCSLEYDQVLN
Sbjct: 364 QRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYDQVLN 423

Query: 430 LSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKK 489
           LSVVDL+V SGLLESKSAARRLLKQGGLYLNN RVDSESK++EA+DI+DGKVLLLSAGKK
Sbjct: 424 LSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLSAGKK 483

Query: 490 NKVVVRIS 494
           NK+VVRIS
Sbjct: 484 NKMVVRIS 491

[Close]

BLAST of CSPI04G04300 vs. TAIR 10
Match: AT3G02660.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 655.2 bits (1689), Expect = 4.1e-188
Identity = 345/506 (68.18%), Postives = 399/506 (78.85%), Query Frame = 0


Query: 6   FASSSSSCYTSKAFLLSQRSFFFSTISSKPSSSFFFKPTSRFFSSSSPTSLHYS------ 65
           FAS S     S+ FL   R         K S++FF +       S+S T+L  S      
Sbjct: 9   FASRSILPICSRTFLSPLRVASLLVFPEKSSATFFRRVQVPHLFSTSTTTLFSSVKCSIH 68

Query: 66  PTPSPPT--------NVIQILEQRGLLDSITSDNLRSA----SLSPLKVYCGFDPTAQSL 125
            T SP T        NV+ ILE+RGLL+SITS+NLRSA     ++PL+VYCGFDPTA+SL
Sbjct: 69  STSSPETENQAVFRPNVVDILEERGLLESITSENLRSACSDPKVAPLRVYCGFDPTAESL 128

Query: 126 HLGNLLGLIVLSWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNI 185
           HLGNLLG+IVLSWF+RCGH  V LIGGAT RVGDPSGKSLERPELD  TLE N  GIT I
Sbjct: 129 HLGNLLGIIVLSWFQRCGHQAVGLIGGATGRVGDPSGKSLERPELDADTLEKNIAGITKI 188

Query: 186 ITKILGNSGSDSDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLES 245
           I KILG++ S      ++VI NNYDWWK+   LDFL  VGRFARVG+M+AKESV++RLES
Sbjct: 189 IIKILGSNPSPGG---SYVIFNNYDWWKDMTMLDFLNKVGRFARVGTMMAKESVKKRLES 248

Query: 246 EQGMSYTEFTYQLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYG 305
           EQGMSYTEFTYQLLQ YDFLHL++NEG++VQIGGSDQWGNITAGT+LIRK L   +  YG
Sbjct: 249 EQGMSYTEFTYQLLQAYDFLHLFKNEGINVQIGGSDQWGNITAGTDLIRKILQAEEAAYG 308

Query: 306 LTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEE 365
           LTFPLLLK+DGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPD DV+RFL+ LTFL ++E
Sbjct: 309 LTFPLLLKNDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDVDVIRFLKTLTFLSLDE 368

Query: 366 IQDLESQMVKPGYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIE 425
           I+ LE QM KPGYVPNTAQ +LAEEVTRFVHGE+GL+EA+KATEAL+PGAETKLDW  IE
Sbjct: 369 IKILEDQMSKPGYVPNTAQIKLAEEVTRFVHGEEGLKEAIKATEALRPGAETKLDWNLIE 428

Query: 426 GIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRV 485
            IAED+P CSL  D+V  LS+VDLSV++GL ESKSAARR+LKQGG Y+NN RVD E+KRV
Sbjct: 429 RIAEDIPSCSLPIDRVSGLSIVDLSVSAGLFESKSAARRMLKQGGFYMNNERVDDENKRV 488

Query: 486 EAVDIVDGKVLLLSAGKKNKVVVRIS 494
           +  DIV+G+ L+LSAGKKNKVVVRIS
Sbjct: 489 DEEDIVEGRGLVLSAGKKNKVVVRIS 511

The following BLAST results are available for this feature:

BLAST of CSPI04G04300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M876	5.8e-187	68.18	Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
Q6MC06	2.4e-132	52.46	Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=...	[more]
A5UUZ9	2.9e-93	42.89	Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV...	[more]
A7NK64	6.4e-93	43.49	Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=3...	[more]
Q8A2S5	1.9e-92	43.69	Tyrosine--tRNA ligase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2...	[more]

BLAST of CSPI04G04300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU70	4.8e-277	99.80	Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1	[more]
A0A5D3CZD9	6.7e-271	97.16	Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13...	[more]
A0A1S4E026	6.7e-271	97.16	Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103494447 PE=3 SV=1	[more]
A0A6J1FW55	1.1e-228	84.43	Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1	[more]
A0A6J1CIM7	1.4e-228	85.37	Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1	[more]

BLAST of CSPI04G04300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146892.1	1.0e-276	99.80	tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN53185.1...	[more]
XP_016901583.1	1.4e-270	97.16	PREDICTED: tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KA...	[more]
XP_038892591.1	2.8e-255	93.13	tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida...	[more]
XP_038892604.1	1.3e-252	92.86	tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida...	[more]
XP_022942360.1	2.2e-228	84.43	tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]

BLAST of CSPI04G04300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02660.1	4.1e-188	68.18	Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002307	Tyrosine-tRNA ligase	PRINTS	PR01040	TRNASYNTHTYR	coord: 251..273 score: 47.25 coord: 230..245 score: 51.67 coord: 106..128 score: 34.2 coord: 286..298 score: 46.92
IPR002307	Tyrosine-tRNA ligase	TIGRFAM	TIGR00234	TIGR00234	coord: 66..491 e-value: 9.9E-106 score: 352.2
IPR002307	Tyrosine-tRNA ligase	CDD	cd00805	TyrRS_core	coord: 94..376 e-value: 4.34435E-108 score: 320.322
IPR002305	Aminoacyl-tRNA synthetase, class Ic	PFAM	PF00579	tRNA-synt_1b	coord: 92..392 e-value: 2.2E-72 score: 243.8
IPR036986	RNA-binding S4 domain superfamily	GENE3D	3.10.290.10		coord: 398..493 e-value: 1.6E-21 score: 78.0
IPR002942	RNA-binding S4 domain	PFAM	PF01479	S4	coord: 434..472 e-value: 1.3E-5 score: 24.8
IPR002942	RNA-binding S4 domain	CDD	cd00165	S4	coord: 433..492 e-value: 9.87939E-7 score: 44.1626
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 51..292 e-value: 5.4E-93 score: 313.3
None	No IPR available	GENE3D	1.10.240.10		coord: 293..397 e-value: 1.3E-35 score: 123.8
None	No IPR available	PROSITE	PS50889	S4	coord: 426..492 score: 11.680996
None	No IPR available	SUPERFAMILY	55174	Alpha-L RNA-binding motif	coord: 395..491
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 67..392
IPR024088	Tyrosine-tRNA ligase, bacterial-type	PANTHER	PTHR11766	TYROSYL-TRNA SYNTHETASE	coord: 65..492
IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	PROSITE	PS00178	AA_TRNA_LIGASE_I	coord: 102..112
IPR024107	Tyrosine-tRNA ligase, bacterial-type, type 1	HAMAP	MF_02006	Tyr_tRNA_synth_type1	coord: 66..492 score: 40.179962

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G04300.1	CSPI04G04300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006437	tyrosyl-tRNA aminoacylation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004831	tyrosine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding