CSPI04G04030 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G04030
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	gibberellin 20-oxidase-like protein
Location	Chr4: 2554662 .. 2556152 (-)
RNA-Seq Expression	CSPI04G04030
Synteny	CSPI04G04030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTAGATCAGCACTATATATCTCAAGAATCAGAATGGTAGTAGTACGAACTATAGCAAGCCAGTTTAATTCCTAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGTCATCCTAAACTCTATTTCAATCTCAAATCCTCCCGGATTTAATTATTATGATAAAATTACAAATTTGATTAAAGCTTAAGAATCGTTCTATTTAAAATATCTTCGGCAAGTTAAATTTGTTTGCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGATGAGAGAGTTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGTATGGACGTTTGGAATGAAAATAGGTATTCATTCTTCTCGTATTAGGATTGGTTGCTGGCTCATGCATAATGCATACATATATGTTATAGTTTTTAAACTTTCAATATGCATATCTCAATTTATGAACTTGGAACAAAAGACAACTATGATGATCTCTTGATGACTTACACATGTACAAGTATAAGTGTATGTAAGATAAAGTTATGTTGGGTTTGCTATACTCGAATCTACCTTATGTGGCTTCAAATATTGCCTATATGTACTTGCATATAATGGATATACTCAAAAG

mRNA sequence

CCTAGATCAGCACTATATATCTCAAGAATCAGAATGGTAGTAGTACGAACTATAGCAAGCCAGTTTAATTCCTAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGATGAGAGAGTTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGTATGGACGTTTGGAATGAAAATAGGTATTCATTCTTCTCGTATTAGGATTGGTTGCTGGCTCATGCATAATGCATACATATATGTTATAGTTTTTAAACTTTCAATATGCATATCTCAATTTATGAACTTGGAACAAAAGACAACTATGATGATCTCTTGATGACTTACACATGTACAAGTATAAGTGTATGTAAGATAAAGTTATGTTGGGTTTGCTATACTCGAATCTACCTTATGTGGCTTCAAATATTGCCTATATGTACTTGCATATAATGGATATACTCAAAAG

Coding sequence (CDS)

ATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTCTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGATGAGAGAGTTTGCTGGAATTGATTTGGAGTGA

Protein sequence

MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYRKIVGVPMREFAGIDLE*

Homology

[Close]

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-68
Identity = 150/333 (45.05%), Postives = 211/333 (63.36%), Query Frame = 0


Query: 8   MHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAV 67
           M  EL V D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y+KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVT--QFR 127
            E   E K+K+GAS YTPRF  SP+ ES +  GP+F  YAS     +  F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDF--YASAKSSVD-AFSDQATDEEFS 120

Query: 128 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L + GD+L  K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++++ G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQD---VKRFSLAFFLIFKDDDRELYAPSEVVG--EGNTRIYK 307
            AW+N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAYRENNYR---KIVGVPMREFAGID 321
            F   +Y  +RE+N +   + VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 4.3e-29
Identity = 89/314 (28.34%), Postives = 151/314 (48.09%), Query Frame = 0


Query: 11  ELLVMD----MNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELL-T 70
           EL V+D    ++  E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEETKEGKLKVGA-SWYTP---RFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TIL+QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.1e-27
Identity = 87/280 (31.07%), Postives = 130/280 (46.43%), Query Frame = 0


Query: 33  KACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVG-----ASWYTPRF 92
           +AC   G F + NHG+  EL        D   T    E +    + G     AS +T RF
Sbjct: 64  EACERHGFFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRF 123

Query: 93  RLS-PYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL 152
               P+ E+  F     SD A  + +     G+   +  ++   Y S M  LS  IM++L
Sbjct: 124 ASKLPWKETLSFRSCP-SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 183

Query: 153 LKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGG 212
            +++G  +    Y   F   +  +R+N Y P     E    G H D + +TIL QD +GG
Sbjct: 184 GESLG--VGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGG 243

Query: 213 IQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLI 272
           +Q+ ++    W  +RP  DA +VNIGD   A SN R +S  HR V+   V R SLAFFL 
Sbjct: 244 LQVHTE--GRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLC 303

Query: 273 FKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 307
             + D+ +  P  +V   N R Y  F+ +    + + +YR
Sbjct: 304 -PEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYR 337

[Close]

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.0e-27
Identity = 87/280 (31.07%), Postives = 130/280 (46.43%), Query Frame = 0


Query: 33  KACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVG-----ASWYTPRF 92
           +AC   G F + NHG+  EL        D   T    E +    + G     AS +T RF
Sbjct: 64  EACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRF 123

Query: 93  RLS-PYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL 152
               P+ E+  F     SD A  + +     G+   +  ++   Y S M  LS  IM++L
Sbjct: 124 ASKLPWKETLSFRSCP-SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 183

Query: 153 LKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGG 212
            +++G  +    Y   F   +  +R+N Y P     E    G H D + +TIL QD +GG
Sbjct: 184 GESLG--VGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGG 243

Query: 213 IQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLI 272
           +Q+ ++    W  +RP  DA +VNIGD   A SN R +S  HR V+   V R SLAFFL 
Sbjct: 244 LQVHTE--GRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLC 303

Query: 273 FKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 307
             + D+ +  P  +V   N R Y  F+ +    + + +YR
Sbjct: 304 -PEMDKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYR 337

[Close]

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.2e-27
Identity = 90/295 (30.51%), Postives = 137/295 (46.44%), Query Frame = 0


Query: 30  SLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKE--GKLKVGASWYTPRF 89
           ++ +A +EWG F + NHGV +EL +++R     L   +P ETKE  G L     W  P  
Sbjct: 72  AMARAASEWGFFQLTNHGVGRELMEEMRREQARLF-RLPFETKEKAGLLNGSYRWGNPTA 131

Query: 90  RLSPYIE-SFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL 149
               ++  S  F  P  S   +D  F +      +T  R ++ E    M  ++  +   L
Sbjct: 132 TSLRHLSWSEAFHVPLASISGADCDFGD------LTSLRGVMQEVAEAMSRVANTVAAAL 191

Query: 150 LKTM-----GDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTILFQ 209
            + +     G      ++ +       +LR+NRY       +      HTD   +T+L Q
Sbjct: 192 AEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQ 251

Query: 210 DEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSL 269
           D++GG+ +   +   WV VRP  DAL+VNIGD  QAWSN R +S EH+VV      R S+
Sbjct: 252 DQVGGLHL--MKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSV 311

Query: 270 AFFLIFKDDDRELYAPSEVVGE-GNTRIYKPFSTKEYRAYRENNYR---KIVGVP 313
           A+FL    D         +VG  G    Y+ F+  EYR   + + R   K +G+P
Sbjct: 312 AYFLCPSYD--------SLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLP 349

[Close]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL
Match: A0A0A0KW97 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.1e-184
Identity = 319/322 (99.07%), Postives = 321/322 (99.69%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNYRKIVGVP+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL
Match: A0A5D3D2E7 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00730 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.1e-168
Identity = 293/322 (90.99%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNY K+VGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL
Match: A0A1S3BYJ4 (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.1e-168
Identity = 293/322 (90.99%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNY K+VGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL
Match: A0A5A7TME4 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004080 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.5e-167
Identity = 291/322 (90.37%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRS EHRVVLKQ+ +RF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNY K+VGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.9e-102
Identity = 196/312 (62.82%), Postives = 241/312 (77.24%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V+D++  + LSES   SL  ACNEWG FYI NHGVS E YQKL ++ DEL   +P++
Sbjct: 9   ELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-RLPQD 68

Query: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130
           T   K+KVG S Y PRF  SP+ ESFK  GP+FS  AS    TE + G   TQF  LL+E
Sbjct: 69  T---KIKVGISSYVPRFTASPFFESFKLSGPDFS--ASAQLLTEALLGHHNTQFSNLLEE 128

Query: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKH 190
           YGS M +LS+RI+KLLLK +GDN E+KFY+ EF NC+GYLRIN Y+PR+SNEE+E  GKH
Sbjct: 129 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 188

Query: 191 TDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRV 250
           TDISCVTILFQD++GG+QM+SK G EW+D+RP E+AL+VNIGDF+QAWSNERLRSAEHRV
Sbjct: 189 TDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRV 248

Query: 251 VLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR---K 310
           VL+Q+VKRFS+AFF  + DD+ E+YAPSEVVGEGN R+Y+ FSTKEYR +R    R   +
Sbjct: 249 VLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYE 308

Query: 311 IVGVPMREFAGI 320
            VG  ++EFAGI
Sbjct: 309 KVGANVKEFAGI 310

[Close]

BLAST of CSPI04G04030 vs. NCBI nr
Match: XP_004146911.1 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_014948 [Cucumis sativus])

HSP 1 Score: 652.5 bits (1682), Expect = 1.9e-183
Identity = 319/322 (99.07%), Postives = 321/322 (99.69%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNYRKIVGVP+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 600.5 bits (1547), Expect = 8.5e-168
Identity = 293/322 (90.99%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNY K+VGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. NCBI nr
Match: KAA0044653.1 (gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 597.4 bits (1539), Expect = 7.2e-167
Identity = 291/322 (90.37%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRS EHRVVLKQ+ +RF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAGIDLE 323
           RENNY K+VGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CSPI04G04030 vs. NCBI nr
Match: XP_038876311.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 561.2 bits (1445), Expect = 5.7e-156
Identity = 274/318 (86.16%), Postives = 294/318 (92.45%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MS+ESIEM+EEL VMD++ C+ LSESSR  LYKACNEWGHFYI+NHGVSKELY KLRAVT
Sbjct: 1   MSEESIEMYEELPVMDIHCCDDLSESSRSCLYKACNEWGHFYIRNHGVSKELYLKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLT   EE KEGKLK G SWYTPRF LSPYIESFKFLGPNFS  ASDLGFTEQVFGQR
Sbjct: 61  DELLT---EERKEGKLKEGVSWYTPRFILSPYIESFKFLGPNFSASASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQ   LLDEYGSIM+ELSR+IMKLLLK MGDNLE+KFYESEFS CNGY+RINRYAPRNS
Sbjct: 121 VTQLSNLLDEYGSIMIELSRKIMKLLLKIMGDNLENKFYESEFSKCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGG+QMKSKQGDEW+DV+PLEDALLVNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGLQMKSKQGDEWIDVKPLEDALLVNIGDFMQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+VKRFSLAFFLIF DDDRE+YAPSEVVGEGNTR+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPMREFAG 319
           RE NY K+VG P+REFAG
Sbjct: 301 REYNYGKVVGTPLREFAG 315

[Close]

BLAST of CSPI04G04030 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 380.9 bits (977), Expect = 1.1e-101
Identity = 196/312 (62.82%), Postives = 241/312 (77.24%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V+D++  + LSES   SL  ACNEWG FYI NHGVS E YQKL ++ DEL   +P++
Sbjct: 9   ELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-RLPQD 68

Query: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130
           T   K+KVG S Y PRF  SP+ ESFK  GP+FS  AS    TE + G   TQF  LL+E
Sbjct: 69  T---KIKVGISSYVPRFTASPFFESFKLSGPDFS--ASAQLLTEALLGHHNTQFSNLLEE 128

Query: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKH 190
           YGS M +LS+RI+KLLLK +GDN E+KFY+ EF NC+GYLRIN Y+PR+SNEE+E  GKH
Sbjct: 129 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 188

Query: 191 TDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRV 250
           TDISCVTILFQD++GG+QM+SK G EW+D+RP E+AL+VNIGDF+QAWSNERLRSAEHRV
Sbjct: 189 TDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRV 248

Query: 251 VLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR---K 310
           VL+Q+VKRFS+AFF  + DD+ E+YAPSEVVGEGN R+Y+ FSTKEYR +R    R   +
Sbjct: 249 VLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYE 308

Query: 311 IVGVPMREFAGI 320
            VG  ++EFAGI
Sbjct: 309 KVGANVKEFAGI 310

[Close]

BLAST of CSPI04G04030 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 4.0e-75
Identity = 143/326 (43.87%), Postives = 212/326 (65.03%), Query Frame = 0


Query: 7   EMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 66
           E+H  L +  ++  + +  S   SL +AC EWG FY+ NHG+SKE++ K+ +++ ++  A
Sbjct: 3   ELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA 62

Query: 67  VPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYA---SDLGFTEQVFGQRVTQ 126
                 E KLK+G   YTPR+  SPY ES    GP+FSD A   +D+ F +        +
Sbjct: 63  ----PLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQD----HHKPE 122

Query: 127 FRKLLDEYGSIMMELSRRIMK-LLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP----R 186
            R+ + EYG+ M ELS+R++K LL+ T+GD    + Y+++FSNC+GYLR+  Y P     
Sbjct: 123 LRETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVE 182

Query: 187 NSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAW 246
              E +E  G HTD+SC+TI++QD +GG+QM+SK+G +W+D+ P  D L+VNIGD +QAW
Sbjct: 183 KQEELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEG-KWIDINPCNDFLVVNIGDLMQAW 242

Query: 247 SNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYR 306
           SN RLRS+EHRVVL++ V R SLAFFL F +D++ + AP E+VGEG  R YK F   EY 
Sbjct: 243 SNGRLRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYL 302

Query: 307 AYRENNYR---KIVGVPMREFAGIDL 322
            +R++N     + +G  +++FAG+ L
Sbjct: 303 KFRQSNEEGKFEKIGFTVKDFAGLKL 318

[Close]

BLAST of CSPI04G04030 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 150/333 (45.05%), Postives = 211/333 (63.36%), Query Frame = 0


Query: 8   MHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAV 67
           M  EL V D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y+KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVT--QFR 127
            E   E K+K+GAS YTPRF  SP+ ES +  GP+F  YAS     +  F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDF--YASAKSSVD-AFSDQATDEEFS 120

Query: 128 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L + GD+L  K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++++ G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQD---VKRFSLAFFLIFKDDDRELYAPSEVVG--EGNTRIYK 307
            AW+N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAYRENNYR---KIVGVPMREFAGID 321
            F   +Y  +RE+N +   + VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of CSPI04G04030 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.5e-53
Identity = 99/202 (49.01%), Postives = 143/202 (70.79%), Query Frame = 0


Query: 128 LDEYGSIMMELSRRIMK-LLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP----RNSNE 187
           + EYG+ M ELS+R++K LL+ T+GD    + Y+++FSNC+GYLR+  Y P        E
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 188 EIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNER 247
            +E  G HTD+SC+TI++QD +GG+QM+SK+G +W+D+ P  D L+VNIGD +QAWSN R
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEG-KWIDINPCNDFLVVNIGDLMQAWSNGR 120

Query: 248 LRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRE 307
           LRS+EHRVVL++ V R SLAFFL F +D++ + AP E+VGEG  R YK F   EY  +R+
Sbjct: 121 LRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 180

Query: 308 NNYR---KIVGVPMREFAGIDL 322
           +N     + +G  +++FAG+ L
Sbjct: 181 SNEEGKFEKIGFTVKDFAGLKL 200

[Close]

BLAST of CSPI04G04030 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 131.3 bits (329), Expect = 1.4e-30
Identity = 87/319 (27.27%), Postives = 153/319 (47.96%), Query Frame = 0


Query: 10  EELLVMDMNSCE---HLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 69
           +E+  +D++S E   H   +    + +AC  WG F + NHG+   L  ++     E    
Sbjct: 30  DEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 89

Query: 70  VPEETKEGKL-KVGASWYTPR---FRLSPYIESFKFL---------GPNFSDYASDLGFT 129
             EE ++ K  +V    Y        +  + E F F           P   D  ++L   
Sbjct: 90  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPED--TELRKL 149

Query: 130 EQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL---LKTMGDNLEDKFYESEFSNCNGYL 189
              + Q  + FR++  EY   + +L+ R+++L+   L   GD L   F E        +L
Sbjct: 150 TNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQ-----TSFL 209

Query: 190 RINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVN 249
           R N Y P  + E     G+H D   +T+L QD +GG+Q+  +   +W+ V+P+ DAL++N
Sbjct: 210 RFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIIN 269

Query: 250 IGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYK 309
           +G+ +Q W+N+   SAEHRVV+    +RFS+ FF  F   +  +    E++ E N   YK
Sbjct: 270 MGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFF-FFPSHEANIEPLEELISEENPPCYK 329

[Close]

BLAST of CSPI04G04030 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 130.2 bits (326), Expect = 3.0e-30
Identity = 89/314 (28.34%), Postives = 151/314 (48.09%), Query Frame = 0


Query: 11  ELLVMD----MNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELL-T 70
           EL V+D    ++  E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEETKEGKLKVGA-SWYTP---RFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TIL+QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

The following BLAST results are available for this feature:

BLAST of CSPI04G04030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.1e-68	45.05	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
O49561	4.3e-29	28.34	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
O04706	3.1e-27	31.07	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
O04707	4.0e-27	31.07	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
Q7XP65	5.2e-27	30.51	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of CSPI04G04030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW97	9.1e-184	99.07	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A5D3D2E7	4.1e-168	90.99	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BYJ4	4.1e-168	90.99	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
A0A5A7TME4	3.5e-167	90.37	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1CI26	3.9e-102	62.82	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]

BLAST of CSPI04G04030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146911.1	1.9e-183	99.07	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical p...	[more]
XP_008453897.1	8.5e-168	90.99	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberell...	[more]
KAA0044653.1	7.2e-167	90.37	gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa]	[more]
XP_038876311.1	5.7e-156	86.16	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
XP_022141254.1	1.1e-101	62.82	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]

BLAST of CSPI04G04030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	4.0e-75	43.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.5e-69	45.05	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	1.5e-53	49.01	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	1.4e-30	27.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G21200.1	3.0e-30	28.34	gibberellin 2-oxidase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..73 e-value: 4.9E-9 score: 37.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..307 e-value: 2.3E-63 score: 216.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..265 e-value: 3.7E-21 score: 75.5
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..311
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 5..311
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 30..305
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..267 score: 11.229484

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G04030.1	CSPI04G04030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding