CSPI04G03620 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G03620
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSalutaridinol 7-O-acetyltransferase-like
LocationChr4: 2204412 .. 2205734 (+)
RNA-Seq ExpressionCSPI04G03620
SyntenyCSPI04G03620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTGACATTTTTGTCCAGAGATACTATCAAGCCTTCTTCTCCAACTCCCCTAACCTTTGAATCTTTTCATCTTTTGTGTATGGAGGAAACGTCTCCTTTGGTTTATACTCCTCTTTTTCTTTTCTATCCGAGGATTAGTAATGATGATGATTCAGGAGAGTATAATCCAGAAATTGATACACTAAAAAACTCTCTATCTGAAAGTATAAATCGTTTCTTGTTTCTTGAAGGAGAAATCGTTGGTGAATCCATTCGTTGCAACTATGAAGAGATCATCTTTGTCAAAGCAAAAGTTACTGGTAAGATATCTGAAATCTTCGTGGGATCAAATAACGATACTTCTTTAATGAAACTCGTCATGTATTCTACGTTCAACACTGACCCAGATGATAAATATGCTCTAATTGGTGTACAAGCCAACATTGTGGAGTGTGGTGGTGTTGTGATATCTCTTTGTTTGTTACACGAAGTTATCGATCCAACAACTCTAAGTCATTTTCTTAGATCGTGGTTCGATATTAATACGTCGTCTTTCAATTGTCTTCCTAGTATGGCCTGTTCTGACTTCGGACCACTCTTTCCACTCTTCGATCCACAACAAAAACAAGTCTTGCTTTCCTCCAACAATAATTCTCAACAAAAAGCTTCTTTCCAAAGCCTTGTGTTCAAAGACGATGAAATCCCAATTCTCAAAGATAGAGCAAAATCCAGTGATGTCCAAAATCCAACATGTGTGGAAGTGCTTTTAGGGTCCCTTTGGAAATGTATATTGGAAGTTGTATTATTGCAAAGTACAACAAATTCTGATCGGCCATCAATTTTGACACATGCAATTATAAACTTGAACACCAAAATGCTATCTTCTAATTCATTGCCAAGATCATTCTCAGTGGGGAATTTTTGGTGGATTGAGGTAGCTGATCATTATGTAGAAAATGAGAAAACCCAAATGGAGCTAAGTAATTTGGTGAGATTTTTAAGAGAGTCATTTCAAGAAATGAATGGTGATAGTTATTCTTGGTTAAAAAGTTTGGTGGGAAATCAAAGAGATAGAGTTATCTCCAAGTTGTTATCAGCTGAAATAATCCCAAAATTGTACATATGCACAAATTGGGAAAACATAGATCAATTGAATGAGTTTGGATGGGGAAATACCATATGGATCGAATCGGATACAACGTCAAAAAACATTGGTATTTTGTTGCGCACCATAAGAGATAATGAAATAGAGATATGGATGGTACTTGATGAGGAAGAAATAGAACTTTTAGTACAAAATGAAGAATTTTGTGGTTTTGTGAATCTAACTTTCCCTTAG

mRNA sequence

ATGGAGGTGACATTTTTGTCCAGAGATACTATCAAGCCTTCTTCTCCAACTCCCCTAACCTTTGAATCTTTTCATCTTTTGTGTATGGAGGAAACGTCTCCTTTGGTTTATACTCCTCTTTTTCTTTTCTATCCGAGGATTAGTAATGATGATGATTCAGGAGAGTATAATCCAGAAATTGATACACTAAAAAACTCTCTATCTGAAAGTATAAATCGTTTCTTGTTTCTTGAAGGAGAAATCGTTGGTGAATCCATTCGTTGCAACTATGAAGAGATCATCTTTGTCAAAGCAAAAGTTACTGGTAAGATATCTGAAATCTTCGTGGGATCAAATAACGATACTTCTTTAATGAAACTCGTCATGTATTCTACGTTCAACACTGACCCAGATGATAAATATGCTCTAATTGGTGTACAAGCCAACATTGTGGAGTGTGGTGGTGTTGTGATATCTCTTTGTTTGTTACACGAAGTTATCGATCCAACAACTCTAAGTCATTTTCTTAGATCGTGGTTCGATATTAATACGTCGTCTTTCAATTGTCTTCCTAGTATGGCCTGTTCTGACTTCGGACCACTCTTTCCACTCTTCGATCCACAACAAAAACAAGTCTTGCTTTCCTCCAACAATAATTCTCAACAAAAAGCTTCTTTCCAAAGCCTTGTGTTCAAAGACGATGAAATCCCAATTCTCAAAGATAGAGCAAAATCCAGTGATGTCCAAAATCCAACATGTGTGGAAGTGCTTTTAGGGTCCCTTTGGAAATGTATATTGGAAGTTGTATTATTGCAAAGTACAACAAATTCTGATCGGCCATCAATTTTGACACATGCAATTATAAACTTGAACACCAAAATGCTATCTTCTAATTCATTGCCAAGATCATTCTCAGTGGGGAATTTTTGGTGGATTGAGGTAGCTGATCATTATGTAGAAAATGAGAAAACCCAAATGGAGCTAAGTAATTTGGTGAGATTTTTAAGAGAGTCATTTCAAGAAATGAATGGTGATAGTTATTCTTGGTTAAAAAGTTTGGTGGGAAATCAAAGAGATAGAGTTATCTCCAAGTTGTTATCAGCTGAAATAATCCCAAAATTGTACATATGCACAAATTGGGAAAACATAGATCAATTGAATGAGTTTGGATGGGGAAATACCATATGGATCGAATCGGATACAACGTCAAAAAACATTGGTATTTTGTTGCGCACCATAAGAGATAATGAAATAGAGATATGGATGGTACTTGATGAGGAAGAAATAGAACTTTTAGTACAAAATGAAGAATTTTGTGGTTTTGTGAATCTAACTTTCCCTTAG

Coding sequence (CDS)

ATGGAGGTGACATTTTTGTCCAGAGATACTATCAAGCCTTCTTCTCCAACTCCCCTAACCTTTGAATCTTTTCATCTTTTGTGTATGGAGGAAACGTCTCCTTTGGTTTATACTCCTCTTTTTCTTTTCTATCCGAGGATTAGTAATGATGATGATTCAGGAGAGTATAATCCAGAAATTGATACACTAAAAAACTCTCTATCTGAAAGTATAAATCGTTTCTTGTTTCTTGAAGGAGAAATCGTTGGTGAATCCATTCGTTGCAACTATGAAGAGATCATCTTTGTCAAAGCAAAAGTTACTGGTAAGATATCTGAAATCTTCGTGGGATCAAATAACGATACTTCTTTAATGAAACTCGTCATGTATTCTACGTTCAACACTGACCCAGATGATAAATATGCTCTAATTGGTGTACAAGCCAACATTGTGGAGTGTGGTGGTGTTGTGATATCTCTTTGTTTGTTACACGAAGTTATCGATCCAACAACTCTAAGTCATTTTCTTAGATCGTGGTTCGATATTAATACGTCGTCTTTCAATTGTCTTCCTAGTATGGCCTGTTCTGACTTCGGACCACTCTTTCCACTCTTCGATCCACAACAAAAACAAGTCTTGCTTTCCTCCAACAATAATTCTCAACAAAAAGCTTCTTTCCAAAGCCTTGTGTTCAAAGACGATGAAATCCCAATTCTCAAAGATAGAGCAAAATCCAGTGATGTCCAAAATCCAACATGTGTGGAAGTGCTTTTAGGGTCCCTTTGGAAATGTATATTGGAAGTTGTATTATTGCAAAGTACAACAAATTCTGATCGGCCATCAATTTTGACACATGCAATTATAAACTTGAACACCAAAATGCTATCTTCTAATTCATTGCCAAGATCATTCTCAGTGGGGAATTTTTGGTGGATTGAGGTAGCTGATCATTATGTAGAAAATGAGAAAACCCAAATGGAGCTAAGTAATTTGGTGAGATTTTTAAGAGAGTCATTTCAAGAAATGAATGGTGATAGTTATTCTTGGTTAAAAAGTTTGGTGGGAAATCAAAGAGATAGAGTTATCTCCAAGTTGTTATCAGCTGAAATAATCCCAAAATTGTACATATGCACAAATTGGGAAAACATAGATCAATTGAATGAGTTTGGATGGGGAAATACCATATGGATCGAATCGGATACAACGTCAAAAAACATTGGTATTTTGTTGCGCACCATAAGAGATAATGAAATAGAGATATGGATGGTACTTGATGAGGAAGAAATAGAACTTTTAGTACAAAATGAAGAATTTTGTGGTTTTGTGAATCTAACTTTCCCTTAG

Protein sequence

MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEIDTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKLVMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRVISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEEEIELLVQNEEFCGFVNLTFP*
Homology
BLAST of CSPI04G03620 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.3e-27
Identity = 119/465 (25.59%), Postives = 211/465 (45.38%), Query Frame = 0

Query: 3   VTFLSRDTIKPSSPTPLTFESFHLLCMEETSPL-VYTPLFLFYP-RISNDDDSGEYNPEI 62
           V  +S++TIKP++PTP   ++F+L  +++  PL  Y P+ LFYP   +N   S  ++ ++
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 63  DTLKNSLSESINRFLFLEGEIVGE-SIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMK 122
           D LK+SLS+++  F  + G ++    + C+ + I F K K+ GK+ E    S  D  L +
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFM--SQPDVPLSQ 128

Query: 123 LVMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSS 182
           L+     +     K AL+ VQ N+ +CGG  I   + H++ D  T+S F+RSW     +S
Sbjct: 129 LLPSEVVSASV-PKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTS 188

Query: 183 FNCLPSMACSDFGPLFPLFD------PQQK--------QVLLSS--NNNSQQKASFQSLV 242
            +   + A +D   L P FD      P ++        ++  SS   +    K   +  V
Sbjct: 189 RSGGSTAAVTD-QKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRFV 248

Query: 243 FKDDEIPILKDRAK-----SSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTH 302
           F   +I  ++++ +     +   +  T VEV+   +WK +     ++ST     P  + H
Sbjct: 249 FDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSV-----MKSTPAGFLP--VVH 308

Query: 303 AIINLNTKM------LSSNSLPRSFS---------VGNFWWIEVADHYVENEKTQMELSN 362
             +NL  KM      +S  +L  + S           N     +     E++    EL +
Sbjct: 309 HAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELHD 368

Query: 363 LVRFLRESFQEMNGDSYSWLKSL--VGNQRDRVISKLLSAEIIPKLYICTNWENIDQLN- 422
            +  +R    ++ GD  S  K +    +  D  I K+   E+I   +  ++W  +     
Sbjct: 369 FIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVIN--FWISSWCRMGLYEI 428

BLAST of CSPI04G03620 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.5e-24
Identity = 114/445 (25.62%), Postives = 206/445 (46.29%), Query Frame = 0

Query: 3   VTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEIDT 62
           V  +S+  IKPSSPTP +   ++L  +++T     T L  FY      + S E +   D 
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY----TYNPSHESSKIGDL 65

Query: 63  LKNSLSESINRFLFLEGEIV-GESIRCNYEEIIFVKAKVTGKISEIFV-GSNNDTSLMKL 122
           LKNSLS+++  +    G ++  + I CN E + FV+ ++ G++++I   G +  T L+  
Sbjct: 66  LKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLP 125

Query: 123 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSW-FDINTSS 182
                 + D     +L+ VQ +  +CGG+ I     H+V D  +   F++ W    + S+
Sbjct: 126 TRIIALHED-----SLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLST 185

Query: 183 FNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSS 242
           F+    +  +D      +F P+  ++L + +  S Q+   +  VF  + I  LK +A   
Sbjct: 186 FHKPTPLLTAD-----SIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEY 245

Query: 243 DVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSV 302
            +Q P+ VEV+   L +C           N D P    +AII+    +    +LP++ S+
Sbjct: 246 GIQKPSRVEVVTAFLCQC---------AANCDLPRKKPYAIISA-VNLRPYLALPQN-SI 305

Query: 303 GNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRVISKLL 362
           GN +        + +E    + S L+  LR   Q++  +  S  K    +Q    ++K L
Sbjct: 306 GNIFSFYFC---INDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQE-LTKCL 365

Query: 363 SAEIIPKL--YICTNWENIDQLN-EFGWGNTIWIES-DTTSKNIGILLRTIRDNEIEIWM 422
               I  L  Y C++W      + +FGWG  I +       K++ +L+ +   + IE  +
Sbjct: 366 DQLNISSLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALI 421

Query: 423 VLDEEEIELLVQNEEFCGFVNLTFP 441
            ++E+++    +NEE   F  L  P
Sbjct: 426 TMEEKKMAAFEKNEELRSFAYLDSP 421

BLAST of CSPI04G03620 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-24
Identity = 117/469 (24.95%), Postives = 208/469 (44.35%), Query Frame = 0

Query: 3   VTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSG-EYNPEID 62
           V  +S++TIKP +PTP    ++++  +++ S LVY P+ LFYP  S+ + +G +++ ++ 
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 63  TLKNSLSESINRFLFLEGEIVGE-SIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 122
            LK SLSE++  F  + G +    ++ CN E I F + ++ G++ +  + S+   SL+  
Sbjct: 69  LLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAHLSLL-- 128

Query: 123 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDIN---- 182
            + S   +    K A + VQ N+ +CGG  I  C+ +++ D  T+  F+RS         
Sbjct: 129 -LPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIAR 188

Query: 183 --------TSSFNCLPSMACSDFGPLFPLFDPQQKQVLL-SSNNNSQQKASFQSLVFKDD 242
                   T++ N +PS    D   LFP  +    QV   + ++ S  K   +  VF   
Sbjct: 189 RGSSIAAPTTNQNLVPSF---DSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAA 248

Query: 243 EIPILKDRAKS-----SDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRP---SILTHA 302
           +I   +++ +S          PT VEV+   +WK         S   S  P   S +THA
Sbjct: 249 KITSAREKLQSLMHDKYKCHRPTRVEVVSALIWK---------SAVKSAPPGSISTVTHA 308

Query: 303 IINLNTKMLSSNSLPRSFSVGNFWWI------------------EVADHYVENEKTQMEL 362
            +N   KM   +   +  S GN   +                  +V+    E +    EL
Sbjct: 309 -MNFRKKM---DPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDEL 368

Query: 363 SNLVRFLRESFQEMNGDSYSWLKSLVG--NQRDRVISKLLSAEIIPKLYICTNWENIDQL 422
           S+ V  LR    ++ GD     K +       D  ++K    E        T+W      
Sbjct: 369 SDFVALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFY 428

BLAST of CSPI04G03620 vs. ExPASy Swiss-Prot
Match: Q9FI40 (BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 7.4e-24
Identity = 111/461 (24.08%), Postives = 208/461 (45.12%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           + +  + R+ IKPSSP P   +   L  ++        P+  FY    N  D  E +P+I
Sbjct: 3   LNLEVIQREVIKPSSPAP--HDRLQLSVIDFGIAEACVPMIFFY----NLADLAEKSPDI 62

Query: 61  --DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLM 120
               L++SLS++++RF  L G+  G SI CN E  +F +A+    +SE     N D + +
Sbjct: 63  VSTRLRSSLSQALSRFYPLAGKKEGVSISCNDEGAVFTEARTNLLLSEFL--RNIDINSL 122

Query: 121 KLVMYSTFNTDPDDKYALIGVQANIVECG-GVVISLCLLHEVIDPTTLSHFLRSWFDINT 180
           K+++ +    +  D   L+ VQA     G G+ + +C+ H + D  ++S F+R W     
Sbjct: 123 KILIPTLAPGESLDSRPLLSVQATFFGSGSGLAVGICVSHCICDAASVSTFVRGWAATAR 182

Query: 181 SSFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAK 240
              N   S        + P  D        ++ +  ++K      VF+ D+I  LK  A 
Sbjct: 183 GDSNDELSTPQFAEVAIHPPADISIHGSPFNALSEVREKCVTNRFVFESDKITKLKIVAA 242

Query: 241 SSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAI---INLNTKMLSSNSLP 300
           S  V +PT VE ++  +W+C        +     R +++T ++   + + T +LS +   
Sbjct: 243 SKSVPSPTRVEAVMSLIWRCARNA--SHANLIVPRATMMTQSMDLRLRIPTNVLSPD--- 302

Query: 301 RSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLV--GNQRD 360
              ++GN   ++          +++E+S +V   R+  +E N      +K  V  G+   
Sbjct: 303 ---AIGN---LQGVFFLKRGPGSEIEISEVVAEFRKEKEEFN----EMIKENVNGGHTNT 362

Query: 361 RVISKLLS------AEIIPKL--YICTNW-ENIDQLNEFGWGNTIWI---ESDTTSKNIG 420
            +  K++S      +E+ P +  Y  ++W        +FGWG   W+     D     + 
Sbjct: 363 TLGQKIMSGIANYMSELKPNIDTYTMSSWCRKAFYEVDFGWGRPAWVGLGHQDIQDGVMY 422

Query: 421 ILLRTIRDNE-IEIWMVLDEEEIELLVQNEEFCGFVNLTFP 441
           +LL   +D E +E+W+ + E+++   V ++E   + +L  P
Sbjct: 423 VLLVDAKDGEGVEVWVGIPEQDMAAFVCDQELLSYASLNPP 440

BLAST of CSPI04G03620 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.2e-21
Identity = 108/453 (23.84%), Postives = 208/453 (45.92%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           +EVT   ++ IKP+SP+P   +   L  ++   P +Y     FY  I+   +    N   
Sbjct: 5   LEVT--GKEVIKPASPSPR--DRLQLSILDLYCPGIYVSTIFFYDLITESSEVFSEN--- 64

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
             LK SLSE+++RF  L G I G SI CN E  +F +A+ T  +   F+ + N  SL   
Sbjct: 65  --LKLSLSETLSRFYPLAGRIEGLSISCNDEGAVFTEAR-TDLLLPDFLRNLNTDSLSGF 124

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECG-GVVISLCLLHEVIDPTTLSHFLRSWFDINT-- 180
           +        P   + L+ V+      G GV +S+ + H++ D  +L  F++ W       
Sbjct: 125 LPTLAAGESP-AAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKG 184

Query: 181 SSFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAK 240
            S + +     + + P  P     ++     S++N   K   +  VF+  +I  LK +A 
Sbjct: 185 KSNSTIEFAETTIYPP--PPSHMYEQFPSTDSDSNITSKYVLKRFVFEPSKIAELKHKAA 244

Query: 241 SSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSIL--THAII--NLNTKMLSSNSL 300
           S  V  PT VE ++  +W+C           NS R ++L    A++   ++ ++   +S+
Sbjct: 245 SESVPVPTRVEAIMSLIWRC---------ARNSSRSNLLIPRQAVMWQAMDIRLRIPSSV 304

Query: 301 PRSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLK-SLVGNQRD 360
                +GN   ++      ++ +++ E+  +V   R++ + +N      L+ + +G    
Sbjct: 305 APKDVIGN---LQSGFSLKKDAESEFEIPEIVATFRKNKERVNEMIKESLQGNTIGQSLL 364

Query: 361 RVISKLLSAEIIPKLYICTNWENIDQLN-EFGWGNTIWI--ESDTTSKN-IGILLRTIRD 420
            ++++ +S       YI ++W        +FG G+ +W+   S T   N +G++L   ++
Sbjct: 365 SLMAETVSESTEIDRYIMSSWCRKPFYEVDFGSGSPVWVGYASHTIYDNMVGVVLIDSKE 424

Query: 421 NE-IEIWMVLDEEEIELLVQNEEFCGFVNLTFP 441
            + +E W+ L EE++ + V ++E   +  L  P
Sbjct: 425 GDGVEAWISLPEEDMSVFVDDQELLAYAVLNPP 432

BLAST of CSPI04G03620 vs. ExPASy TrEMBL
Match: A0A0A0KU95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016470 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.5e-253
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPLTFESFHLLC+EETSPLVYTPLFLFYPRISNDDDSGEYNPEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCIEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL
Sbjct: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180
           VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF
Sbjct: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180

Query: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD 240
           NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD
Sbjct: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD 240

Query: 241 VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG 300
           VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG
Sbjct: 241 VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG 300

Query: 301 NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRVISKLLS 360
           NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQ DRVISKLLS
Sbjct: 301 NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQGDRVISKLLS 360

Query: 361 AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE 420
           AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE
Sbjct: 361 AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE 420

Query: 421 EIELLVQNEEFCGFVNLTFP 441
           EIELLVQNEEFCGFVNLTFP
Sbjct: 421 EIELLVQNEEFCGFVNLTFP 440

BLAST of CSPI04G03620 vs. ExPASy TrEMBL
Match: A0A5A7TST4 (Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003720 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.1e-224
Identity = 400/446 (89.69%), Postives = 420/446 (94.17%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVTGKIS+IFVGSNNDTSLMKL
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVTGKISDIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDD--KYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           VMYSTF TDP D  KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS
Sbjct: 121 VMYSTFMTDPADDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180

Query: 181 SFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRA 240
           SFNCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRA
Sbjct: 181 SFNCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRA 240

Query: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPR 300
           KSSDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPR
Sbjct: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPR 300

Query: 301 SFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDR-V 360
           SFSVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ DR V
Sbjct: 301 SFSVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDRSV 360

Query: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420
           ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW
Sbjct: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420

Query: 421 MVLDEEEIELLVQNEEFCGFVNLTFP 441
           MVLDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 MVLDEEEIELLVQTEEFCGFVNLTFP 444

BLAST of CSPI04G03620 vs. ExPASy TrEMBL
Match: A0A5D3D0E8 (Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001090 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.5e-224
Identity = 399/446 (89.46%), Postives = 419/446 (93.95%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVTGKIS+IFVGSNNDTSLMKL
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVTGKISDIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDD--KYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           VMYSTF TDP D  KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS
Sbjct: 121 VMYSTFMTDPADDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180

Query: 181 SFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRA 240
           SFNCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRA
Sbjct: 181 SFNCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRA 240

Query: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPR 300
           KSSDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPR
Sbjct: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPR 300

Query: 301 SFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRD-RV 360
           SFSVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ D  V
Sbjct: 301 SFSVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDGSV 360

Query: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420
           ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW
Sbjct: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420

Query: 421 MVLDEEEIELLVQNEEFCGFVNLTFP 441
           MVLDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 MVLDEEEIELLVQTEEFCGFVNLTFP 444

BLAST of CSPI04G03620 vs. ExPASy TrEMBL
Match: A0A1S3BYP5 (salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494519 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.7e-205
Identity = 372/444 (83.78%), Postives = 391/444 (88.06%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVT                   
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVT------------------- 120

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180
                     D KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF
Sbjct: 121 ---------DDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180

Query: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRAKS 240
           NCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRAKS
Sbjct: 181 NCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRAKS 240

Query: 241 SDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPRSF 300
           SDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPRSF
Sbjct: 241 SDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPRSF 300

Query: 301 SVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRD-RVIS 360
           SVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ D  VIS
Sbjct: 301 SVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDGSVIS 360

Query: 361 KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV 420
           KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV
Sbjct: 361 KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV 414

Query: 421 LDEEEIELLVQNEEFCGFVNLTFP 441
           LDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 LDEEEIELLVQTEEFCGFVNLTFP 414

BLAST of CSPI04G03620 vs. ExPASy TrEMBL
Match: A0A5D3CZ08 (Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001080 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 1.7e-84
Identity = 199/481 (41.37%), Postives = 288/481 (59.88%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPE- 60
           MEV  LS++ + PSSPTP   ++F L  +++ SP++Y PL +FYP  + DD     + E 
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  -IDTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLM 120
            + TLK+SLS++++ F  L G IV +SIRC+ E  +F++A V+  +SEI    NN+  LM
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCSDEGAVFIEATVSCSMSEILKQPNNE-FLM 120

Query: 121 KLVMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           KL+  S   T P ++YA + VQAN+ +CGG+ ISLCLLH+++D TT+  FL+ W  IN  
Sbjct: 121 KLIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRR 180

Query: 181 SFNC--------------------------LPSMACSDFGPLFPLFD-----PQQKQVLL 240
           SF                               M C D+  L  LF      P   ++  
Sbjct: 181 SFTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 SSNNNSQQKASFQSLVFKDDEIPILKDRAKSSDVQNPTCVEVLLGSLWKCILEVVLLQST 300
           + ++N ++K+SFQ  VFK+  I  LK +AKS+DV NPT VEVL G +WKC LE    +  
Sbjct: 241 ALSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLG 300

Query: 301 TNSDRPSILTHAIINLNTKMLSSNSLPRSFSVGNFWWIEVADHYVENEKTQMELSNLVRF 360
             S  PSILTHA +NL  +M     LP  FSVGN +W  VA HY+ ++KTQ+ELS LV  
Sbjct: 301 L-SQIPSILTHA-VNLRKRM--EPPLP-EFSVGNIFWNVVA-HYLADKKTQVELSELVSL 360

Query: 361 LRESFQEMNGDSYSWLKSLVGNQRDRVISKL-----LSAEIIPKLYICTNWENIDQLNE- 420
           +R+SF ++NG   +++K +VGN+    + KL     +    IPKLYICT+W N+D L+E 
Sbjct: 361 IRQSFVDING---NYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMD-LSEV 420

Query: 421 -FGWGNTIWI----ESDTTSKNIGILLRTIRDNEIEIWMVLDEEEIELLVQNEEFCGFVN 438
            FGWG  IWI     S+TT KN+ +LL  I ++ +E W++L+EEE+++L++N+EF  F  
Sbjct: 421 NFGWGKPIWIGSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFAL 470

BLAST of CSPI04G03620 vs. NCBI nr
Match: XP_011654330.1 (salutaridinol 7-O-acetyltransferase [Cucumis sativus] >KGN53108.1 hypothetical protein Csa_014793 [Cucumis sativus])

HSP 1 Score: 884.0 bits (2283), Expect = 5.2e-253
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPLTFESFHLLC+EETSPLVYTPLFLFYPRISNDDDSGEYNPEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCIEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL
Sbjct: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180
           VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF
Sbjct: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180

Query: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD 240
           NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD
Sbjct: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSD 240

Query: 241 VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG 300
           VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG
Sbjct: 241 VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVG 300

Query: 301 NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRVISKLLS 360
           NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQ DRVISKLLS
Sbjct: 301 NFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQGDRVISKLLS 360

Query: 361 AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE 420
           AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE
Sbjct: 361 AEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMVLDEE 420

Query: 421 EIELLVQNEEFCGFVNLTFP 441
           EIELLVQNEEFCGFVNLTFP
Sbjct: 421 EIELLVQNEEFCGFVNLTFP 440

BLAST of CSPI04G03620 vs. NCBI nr
Match: KAA0044621.1 (salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 788.9 bits (2036), Expect = 2.3e-224
Identity = 400/446 (89.69%), Postives = 420/446 (94.17%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVTGKIS+IFVGSNNDTSLMKL
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVTGKISDIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDD--KYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           VMYSTF TDP D  KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS
Sbjct: 121 VMYSTFMTDPADDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180

Query: 181 SFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRA 240
           SFNCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRA
Sbjct: 181 SFNCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRA 240

Query: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPR 300
           KSSDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPR
Sbjct: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPR 300

Query: 301 SFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDR-V 360
           SFSVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ DR V
Sbjct: 301 SFSVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDRSV 360

Query: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420
           ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW
Sbjct: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420

Query: 421 MVLDEEEIELLVQNEEFCGFVNLTFP 441
           MVLDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 MVLDEEEIELLVQTEEFCGFVNLTFP 444

BLAST of CSPI04G03620 vs. NCBI nr
Match: TYK16968.1 (salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 786.6 bits (2030), Expect = 1.1e-223
Identity = 399/446 (89.46%), Postives = 419/446 (93.95%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVTGKIS+IFVGSNNDTSLMKL
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVTGKISDIFVGSNNDTSLMKL 120

Query: 121 VMYSTFNTDPDD--KYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           VMYSTF TDP D  KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS
Sbjct: 121 VMYSTFMTDPADDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180

Query: 181 SFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRA 240
           SFNCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRA
Sbjct: 181 SFNCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRA 240

Query: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPR 300
           KSSDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPR
Sbjct: 241 KSSDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPR 300

Query: 301 SFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRD-RV 360
           SFSVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ D  V
Sbjct: 301 SFSVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDGSV 360

Query: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420
           ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW
Sbjct: 361 ISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIW 420

Query: 421 MVLDEEEIELLVQNEEFCGFVNLTFP 441
           MVLDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 MVLDEEEIELLVQTEEFCGFVNLTFP 444

BLAST of CSPI04G03620 vs. NCBI nr
Match: XP_008453951.1 (PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo])

HSP 1 Score: 723.4 bits (1866), Expect = 1.2e-204
Identity = 372/444 (83.78%), Postives = 391/444 (88.06%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           MEVTFLSRDTIKPSSPTPL+FE+FHL C+EETSPLVYTPLFLFYPR SN+DDSGE +PEI
Sbjct: 1   MEVTFLSRDTIKPSSPTPLSFETFHLFCLEETSPLVYTPLFLFYPR-SNNDDSGESHPEI 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
           DTLK+SLSE++NRFLFLEGE +GESIRCNYEEI+FV+AKVT                   
Sbjct: 61  DTLKSSLSETLNRFLFLEGETIGESIRCNYEEIVFVEAKVT------------------- 120

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180
                     D KYA+IGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF
Sbjct: 121 ---------DDYKYAVIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180

Query: 181 NCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLV--FKDDEIPILKDRAKS 240
           NCLPSMACSDFGPL PLFDP+QKQV  SSNN+SQQ+ASFQSL+  FKDDEI  LKDRAKS
Sbjct: 181 NCLPSMACSDFGPLLPLFDPRQKQVFFSSNNSSQQQASFQSLLCTFKDDEIQFLKDRAKS 240

Query: 241 SDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSD-RPSILTHAIINLNTKMLSSNSLPRSF 300
           SDVQNPTCVEVLLGSLWKCILEVVLLQ TTNSD RPSILTHAIINLN KMLSSNSLPRSF
Sbjct: 241 SDVQNPTCVEVLLGSLWKCILEVVLLQITTNSDHRPSILTHAIINLNIKMLSSNSLPRSF 300

Query: 301 SVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRD-RVIS 360
           SVGNFWWIEVA HY+ NE TQMELSNLVRFLRESFQEMNGD + W+KSLVGNQ D  VIS
Sbjct: 301 SVGNFWWIEVA-HYLANETTQMELSNLVRFLRESFQEMNGDDHCWIKSLVGNQGDGSVIS 360

Query: 361 KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV 420
           KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV
Sbjct: 361 KLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSKNIGILLRTIRDNEIEIWMV 414

Query: 421 LDEEEIELLVQNEEFCGFVNLTFP 441
           LDEEEIELLVQ EEFCGFVNLTFP
Sbjct: 421 LDEEEIELLVQTEEFCGFVNLTFP 414

BLAST of CSPI04G03620 vs. NCBI nr
Match: XP_008453950.1 (PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >TYK16967.1 salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 323.2 bits (827), Expect = 3.6e-84
Identity = 199/481 (41.37%), Postives = 288/481 (59.88%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPE- 60
           MEV  LS++ + PSSPTP   ++F L  +++ SP++Y PL +FYP  + DD     + E 
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  -IDTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLM 120
            + TLK+SLS++++ F  L G IV +SIRC+ E  +F++A V+  +SEI    NN+  LM
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCSDEGAVFIEATVSCSMSEILKQPNNE-FLM 120

Query: 121 KLVMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS 180
           KL+  S   T P ++YA + VQAN+ +CGG+ ISLCLLH+++D TT+  FL+ W  IN  
Sbjct: 121 KLIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRR 180

Query: 181 SFNC--------------------------LPSMACSDFGPLFPLFD-----PQQKQVLL 240
           SF                               M C D+  L  LF      P   ++  
Sbjct: 181 SFTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 SSNNNSQQKASFQSLVFKDDEIPILKDRAKSSDVQNPTCVEVLLGSLWKCILEVVLLQST 300
           + ++N ++K+SFQ  VFK+  I  LK +AKS+DV NPT VEVL G +WKC LE    +  
Sbjct: 241 ALSSNCEEKSSFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAASTKLG 300

Query: 301 TNSDRPSILTHAIINLNTKMLSSNSLPRSFSVGNFWWIEVADHYVENEKTQMELSNLVRF 360
             S  PSILTHA +NL  +M     LP  FSVGN +W  VA HY+ ++KTQ+ELS LV  
Sbjct: 301 L-SQIPSILTHA-VNLRKRM--EPPLP-EFSVGNIFWNVVA-HYLADKKTQVELSELVSL 360

Query: 361 LRESFQEMNGDSYSWLKSLVGNQRDRVISKL-----LSAEIIPKLYICTNWENIDQLNE- 420
           +R+SF ++NG   +++K +VGN+    + KL     +    IPKLYICT+W N+D L+E 
Sbjct: 361 IRQSFVDING---NYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMD-LSEV 420

Query: 421 -FGWGNTIWI----ESDTTSKNIGILLRTIRDNEIEIWMVLDEEEIELLVQNEEFCGFVN 438
            FGWG  IWI     S+TT KN+ +LL  I ++ +E W++L+EEE+++L++N+EF  F  
Sbjct: 421 NFGWGKPIWIGSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFAL 470

BLAST of CSPI04G03620 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 143.7 bits (361), Expect = 3.6e-34
Identity = 123/452 (27.21%), Postives = 212/452 (46.90%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           M V  +SRD IKPSSPTP   + F L  +E+  P ++ P+  FY    + ++S +   ++
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFY----SANNSIKPTEQL 60

Query: 61  DTLKNSLSESINRFLFLEGEIVGE-SIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMK 120
             LK SLSE++  F  L G + G  SI CN     F++A+V   +S + +  ++D SL +
Sbjct: 61  QMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSD-SLQQ 120

Query: 121 LVMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSS 180
           L+  S  +   + +  L+  QA+  ECG + I +C+ H++ D T++  F++SW  I++  
Sbjct: 121 LIPTSVDSI--ETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRG 180

Query: 181 FNCLPSMACSDFGPLFP--LFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAK 240
                     D   +FP   F       ++       Q  S +  +F    I  L+ +A 
Sbjct: 181 SIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLS-KRFIFDSSSIQALQAKAS 240

Query: 241 SSDVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLP-RS 300
           S +V  PT VE +   +WK  ++    ++ + + +PSIL +++      + S  S P   
Sbjct: 241 SFEVNQPTRVEAVSALIWKSAMKAT--RTVSGTSKPSILANSV-----SLRSRVSPPFTK 300

Query: 301 FSVGNF--WWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRV 360
            S+GN   ++   A+  +   K Q  +S  +R  ++ F++++      +  LVGN     
Sbjct: 301 NSIGNLVSYFAAKAEEGINQTKLQTLVSK-IRKAKQRFRDIH------IPKLVGNPNATE 360

Query: 361 ISKLLSAEIIPKL-------YICTNWENIDQL-NEFGWGNTIWIE-SDTTSKNIGILLRT 420
           I      E    +       YI ++         +FGWG  +W+       KNI  LL T
Sbjct: 361 IICSYQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDT 420

Query: 421 IRDNEIEIWMVLDEEEIELLVQNEEFCGFVNL 438
                IE W+ L+E+E+ L  Q+ E   F +L
Sbjct: 421 KEAGGIEAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of CSPI04G03620 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 142.9 bits (359), Expect = 6.2e-34
Identity = 124/446 (27.80%), Postives = 214/446 (47.98%), Query Frame = 0

Query: 3   VTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEIDT 62
           V  LSR+ +KPSSPTP      +L  ++  S  +YT   LFY     +            
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLK 64

Query: 63  LKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKLVM 122
           LK SLS+++  F  L G I+G  + CN E  +F++A+V   +SE F+      SL  L+ 
Sbjct: 65  LKKSLSKTLPIFYPLAGRIIGSFVECNDEGAVFIEARVDHLLSE-FLKCPVPESLELLIP 124

Query: 123 YSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTS-SFN 182
               + +    + ++ +QAN   CGG+VI++C+ H++ D T+L+ F+R W + +      
Sbjct: 125 VEAKSREA-VTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLGIT 184

Query: 183 CLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSSDV 242
            +PS   S+  P  PL D    + +       +     +  VF   +I  L+ +A  + V
Sbjct: 185 LIPSFTASEVFPK-PL-DELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASRNLV 244

Query: 243 QNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSLPRSFSVGN 302
           +NPT VE +    W+C+ +V  L S T   R S+L   ++NL  K+   +SL  + ++GN
Sbjct: 245 KNPTRVEAVTALFWRCVTKVSRLSSLT--PRTSVL-QILVNLRGKV---DSLCEN-TIGN 304

Query: 303 FWWIEVADHYVENEKTQME----LSNLVRFLRESFQEMNGDSYSWLKSLVGNQRDRVISK 362
              + +    ++NE+  +E    + + +R  +E F  +N    S   S +    +  I K
Sbjct: 305 MLSLMI----LKNEEAAIERIQDVVDEIRRAKEIF-SLNCKEMSKSSSRIFELLEE-IGK 364

Query: 363 LLSAEIIPKLYICTNWENIDQLN-EFGWGNTIWIESDTTS--KNIGILLRTIRDNEIEIW 422
           +        L++  +W  +   + +FGWG  +W+    TS  KN+ +L+ T     IE W
Sbjct: 365 VYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAW 424

Query: 423 MVLDEEEIELLVQNEEFCGFVNLTFP 441
           + L EE++ L   ++E     +L  P
Sbjct: 425 ITLTEEQMSLFECDQELLESASLNPP 433

BLAST of CSPI04G03620 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 137.9 bits (346), Expect = 2.0e-32
Identity = 125/457 (27.35%), Postives = 211/457 (46.17%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           M+V  +S++ IKPSSPTP   ++  L   +   P VYT  FLFY +  ND  S E+    
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTK--NDLISQEHTSH- 65

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
             LK SLSE++ +F  L G I G ++ C  E  IFV A+V       F+   +  +L +L
Sbjct: 66  -KLKTSLSETLTKFYPLAGRITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 125

Query: 121 VMYSTFNTD--PDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDI--- 180
           +     +        + L+ V+A    CGG+ I +C+ H++ D  ++S F+RSW      
Sbjct: 126 LPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARG 185

Query: 181 -NTSSFNCLPSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASF-QSLVFKDDEIPILK 240
            N ++    P  A ++F P      P  +   L ++  + +++S  +  VF+  ++  L+
Sbjct: 186 ENDAAAMESPVFAGANFYP------PANEAFKLPADEQAGKRSSITKRFVFEASKVEDLR 245

Query: 241 DRAKSSD-VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNS 300
            +A S + V  PT VE +   +WKC    V    TT  D   ++   + NL +K+    S
Sbjct: 246 TKAASEETVDQPTRVESVTALIWKCF---VASSKTTTCDHKVLV--QLANLRSKI---PS 305

Query: 301 LPRSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEM---------NGDSYSWL 360
           L +  S+GN  +  V        + ++E    VR LR+  +E+         + DS S +
Sbjct: 306 LLQESSIGNLMFSSVVLSIGRGGEVKIE--EAVRDLRKKKEELGTVILDEGGSSDSSSMI 365

Query: 361 KSLVGNQRDRVISKLLSAEIIPKLYICTNWENIDQLN-EFGWGNTIWIESDTTS--KNIG 420
            S + N      S+L      P  Y  ++W  +      FGW + +W+  + +    N+ 
Sbjct: 366 GSKLANLMLTNYSRLSYETHEP--YTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLA 425

Query: 421 ILLRTIRDNEIEIWMVLDEEEIELLVQNEEFCGFVNL 438
           +L+ +     IE ++ L EE +    QN E   F  +
Sbjct: 426 MLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of CSPI04G03620 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 5.8e-32
Identity = 131/454 (28.85%), Postives = 208/454 (45.81%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           M+V  ++++ IKPSS TP   ++  L   +   P VYT  FLFY +    DD        
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTK----DDLISPEQSS 63

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
             LK SL+E++ +F  L G I G +I CN E  +FV A+V       F+ S +  +L +L
Sbjct: 64  HKLKTSLAETLTKFYPLAGRIKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQL 123

Query: 121 VMYSTFNTDPDD---KYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINT 180
           +     + DP +    + L+ V+A    CGG+ I LC+ H++ D T++S F++SW  +  
Sbjct: 124 LPLDVID-DPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 181 SSFNCL---PSMACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKD 240
                +   P  A ++F P  P  +  +  V  ++N  SQ    F   VF   ++  L+ 
Sbjct: 184 GEAGDIVADPVFAAANFYP--PANELFKFPVDENANKISQITKRF---VFSASKLEELRT 243

Query: 241 RAKSSD-VQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLSSNSL 300
           +A S D V  P  VE +   LWK    V    S TN+    +L     NL  K+    SL
Sbjct: 244 KAASEDFVGRPKRVESVTALLWKAF--VAAASSNTNACDMKVLIQP-SNLRPKI---PSL 303

Query: 301 PRSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRES-------FQEMNGDSYSWLKSL 360
                +GN  +  V     + E+ ++E    VR LR+         +E  G S + + S 
Sbjct: 304 LAESLIGNIMFSSVVLGISQQEEIKIE--KAVRDLRKQGDDLQNVIKEEGGSSSAMIGSK 363

Query: 361 VGNQRDRVISKLLSAEIIPKLYICTNWENIDQLN-EFGWGNTIWIESDTTS--KNIGILL 420
           + N      SKL      P  Y  ++W  +      FGWG+ +WI  +     +N+ +L+
Sbjct: 364 LANLMLTNYSKLSYETHEP--YTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLI 423

Query: 421 RTIRDNEIEIWMVLDEEEIELLVQNEEFCGFVNL 438
            +     IE ++ L EE +  L QN E   F ++
Sbjct: 424 DSKDGQGIEAFVTLPEENMLSLEQNTELLAFASV 437

BLAST of CSPI04G03620 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 119/446 (26.68%), Postives = 196/446 (43.95%), Query Frame = 0

Query: 1   MEVTFLSRDTIKPSSPTPLTFESFHLLCMEETSPLVYTPLFLFYPRISNDDDSGEYNPEI 60
           M+V  + ++ IKPS+ TP    +  L  M+   P VY   FLFY +    DD        
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFYTK----DDLISQEQTS 63

Query: 61  DTLKNSLSESINRFLFLEGEIVGESIRCNYEEIIFVKAKVTGKISEIFVGSNNDTSLMKL 120
            TLK SLSE + +F  L G + G +I+   E  +FV+A+V       F+ S +  SL +L
Sbjct: 64  HTLKTSLSEILTKFHPLAGRVNGVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTESLKQL 123

Query: 121 VMYSTFNTDPDDKYALIGVQANIVECGGVVISLCLLHEVIDPTTLSHFLRSWFDINTSSF 180
           +     + +P   + L+ V+A    CGG+ I LC+ H + D  +LS FL++W        
Sbjct: 124 L---PVDDEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARGES 183

Query: 181 NCLPSM-ACSDFGPLFPLFDPQQKQVLLSSNNNSQQKASFQSLVFKDDEIPILKDRAKSS 240
           + + S   CS    L+P  +   K      N  S  K      VF   +I  L+++  S 
Sbjct: 184 DLVASPDFCST--KLYPAANEAIKIPGEVVNRTSVTK----RFVFVASKIEELRNKVASD 243

Query: 241 DVQNPTCVEVLLGSLWKCILEVVLLQSTTNSDRPSILTHAIINLNTKMLS-------SNS 300
            V  PT V+ +   +WKC+     + ++T++ R   L     NL TK+ S        N 
Sbjct: 244 VVPRPTRVQSVTSLIWKCV-----VTASTDTIREKALFQP-ANLRTKIPSLLSENQIGNL 303

Query: 301 LPRSFSVGNFWWIEVADHYVENEKTQMELSNLVRFLRESFQEMNGDSYSWLKSLVGNQRD 360
           L  S  +     +++ +   E +K   +LS LV+    S   +    +  + +   N+ D
Sbjct: 304 LFNSLILDGKAGMDIVETVKELQKRAEDLSGLVQDEEGSSMTIGWRLFGEIINSKFNRED 363

Query: 361 RVISKLLSAEIIPKLYICTNWENIDQLNEFGWGNTIWIESDTTSK--NIGILLRTIRDNE 420
             +  + S   IP    C           FGWG+ +W+    +     + +L+ +     
Sbjct: 364 HDMHSVTSWCKIPLYDAC-----------FGWGSPVWVAGSVSPNLDYVTVLIDSKDGQG 419

Query: 421 IEIWMVLDEEEIELLVQNEEFCGFVN 437
           IE W+ L +E + LL Q+ E   F +
Sbjct: 424 IEAWVTLPQENMLLLEQSTELLAFAS 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94FT49.3e-2725.59Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
A0A2K8FQU51.5e-2425.62Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
I3PLR41.9e-2424.95(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
Q9FI407.4e-2424.08BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=... [more]
O233931.2e-2123.84BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KU952.5e-25399.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016470 PE=3 SV=1[more]
A0A5A7TST41.1e-22489.69Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3D0E85.5e-22489.46Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BYP55.7e-20583.78salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494519... [more]
A0A5D3CZ081.7e-8441.37Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
XP_011654330.15.2e-25399.55salutaridinol 7-O-acetyltransferase [Cucumis sativus] >KGN53108.1 hypothetical p... [more]
KAA0044621.12.3e-22489.69salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa][more]
TYK16968.11.1e-22389.46salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa][more]
XP_008453951.11.2e-20483.78PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo][more]
XP_008453950.13.6e-8441.37PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >TYK16967.1 s... [more]
Match NameE-valueIdentityDescription
AT3G26040.13.6e-3427.21HXXXD-type acyl-transferase family protein [more]
AT1G24420.16.2e-3427.80HXXXD-type acyl-transferase family protein [more]
AT4G15390.12.0e-3227.35HXXXD-type acyl-transferase family protein [more]
AT3G30280.15.8e-3228.85HXXXD-type acyl-transferase family protein [more]
AT5G47950.11.6e-2926.68HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 216..432
e-value: 1.0E-25
score: 92.1
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..204
e-value: 1.4E-33
score: 118.0
IPR003480TransferasePFAMPF02458Transferasecoord: 1..431
e-value: 6.1E-44
score: 150.4
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 5..437
NoneNo IPR availablePANTHERPTHR31623:SF59HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEINcoord: 5..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03620.1CSPI04G03620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups