CSPI04G03270 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G03270
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionervatamin-B-like
LocationChr4: 2022811 .. 2024155 (+)
RNA-Seq ExpressionCSPI04G03270
SyntenyCSPI04G03270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGTAATTAATTTAATTTCATTTATAATCTTTCTAAGCCTAATGCCTTCTAACTAATAATTACCAATGTCTTCTAACTAATAATTATGTGTTATTATGTGTTGTTCGACAGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTCTAATTAAACTATCTAAATTTATCATATGAGTGCATTATTGATTATAATATAATTATGTGTATTGTGTTTGATATTTCACTAATAGCCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGCAAGTGTAGTTAATTTTGTTGTTGTTAGTTATAATTTGGTGCAACATATATAATTAAAAAATGATGATTATTTGCAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

mRNA sequence

ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

Coding sequence (CDS)

ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

Protein sequence

MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVGGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY*
Homology
BLAST of CSPI04G03270 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.3e-95
Identity = 180/350 (51.43%), Postives = 242/350 (69.14%), Query Frame = 0

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M KF I+  +S+A    + ESF+   K+ ESE+SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKF-ILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + NA HV   N M K  KL+LN+FAD+++ EF   Y GS + H    H    GG R  
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKH----HRMFRGGPRGN 120

Query: 123 G-FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 182
           G FMYE+   +P+S+DWR+KGAV ++K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSE
Sbjct: 121 GTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSE 180

Query: 183 QEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTID 242
           QE+VDCD     GC GG  + AFEFI Q GGIT E NYPY A +G C     N   V+ID
Sbjct: 181 QELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSID 240

Query: 243 GYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGY 302
           G+E VP+N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+   GS CG  +DH V +VGY
Sbjct: 241 GHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVF-TGS-CGTELDHGVAIVGY 300

Query: 303 GSDEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           G+  +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM+ S+P+K
Sbjct: 301 GTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CSPI04G03270 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.1e-94
Identity = 173/348 (49.71%), Postives = 233/348 (66.95%), Query Frame = 0

Query: 4   IKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           +K L+  V+S++    +  SF+   KD ESE+SL  LY+RW SHH +SR+  E HKRF +
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 64  CQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGG 123
            + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H+        G G    
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGT--- 122

Query: 124 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 183
           FMYE+   +P+S+DWR+KGAV  +K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSEQE
Sbjct: 123 FMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 182

Query: 184 VVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 243
           +VDCD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+
Sbjct: 183 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGH 242

Query: 244 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 303
           E VP N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+      C   ++H V +VGYG+
Sbjct: 243 ENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCNTDLNHGVAIVGYGT 302

Query: 304 DEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
             +G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  S+P+K
Sbjct: 303 TVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CSPI04G03270 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-91
Identity = 177/350 (50.57%), Postives = 233/350 (66.57%), Query Frame = 0

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+ S+      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L     G  +  
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQ-- 120

Query: 123 GFMY--ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLS 182
            FMY  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLS
Sbjct: 121 -FMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLS 180

Query: 183 EQEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 242
           EQE+VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTI
Sbjct: 181 EQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTI 240

Query: 243 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 302
           DG+E VP+N+E AL+KAVA+QPV+VA+ +  SDF+FY EG+   GS CG  ++H V  VG
Sbjct: 241 DGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF-TGS-CGTELNHGVAAVG 300

Query: 303 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           YGS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 YGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03270 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-91
Identity = 173/347 (49.86%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 5   KFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKIC 64
           K L+  V+S +    +  SF+   KD  SE+SL  LY+RW SHH +SR+  E HKRF + 
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGF 124
           + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H        G     G F
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFR---GTPHENGAF 123

Query: 125 MYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 184
           MYE+   +P S+DWR+KGAV  +K+QG CGSCWAF+ V AVE I+QIKTN+LV+LSEQE+
Sbjct: 124 MYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQEL 183

Query: 185 VDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 244
           VDCD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+E
Sbjct: 184 VDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHE 243

Query: 245 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 304
            VP N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+      C   ++H V +VGYG+ 
Sbjct: 244 NVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCSTDLNHGVAIVGYGTT 303

Query: 305 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
            +G +YWI+RN +G +WG +GY++MQR     +G+CG+AM PS+P+K
Sbjct: 304 VDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CSPI04G03270 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.2e-91
Identity = 170/347 (48.99%), Postives = 232/347 (66.86%), Query Frame = 0

Query: 8   IVFVVSIAFTSHL--CESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKICQ 67
           + F+V I+F S L   + F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF + +
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGFM 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L     G    GGFM
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS---GGFM 122

Query: 128 YERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 187
           YE    +PSS+DWREKGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+V
Sbjct: 123 YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELV 182

Query: 188 DCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYE 247
           DCD +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E
Sbjct: 183 DCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHE 242

Query: 248 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 307
            VP+N+E  L+KAVAHQPV+VA+ +  SDF+ Y EG+      CG +++H VV+VGYG  
Sbjct: 243 HVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYGET 302

Query: 308 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           + G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03270 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.8e-170
Identity = 292/349 (83.67%), Postives = 320/349 (91.69%), Query Frame = 0

Query: 2   TMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T++KFLIV +V IA TSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVG 121
           K+ +DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    G VG
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT---GHVG 122

Query: 122 GFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 181
           GFMYE A+ IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQ
Sbjct: 123 GFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 182

Query: 182 EVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 241
           EVVDCDY+ GGCRGG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERV IDGY
Sbjct: 183 EVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGY 242

Query: 242 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 301
           E VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGS
Sbjct: 243 ENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGS 302

Query: 302 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03270 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.1e-170
Identity = 292/349 (83.67%), Postives = 319/349 (91.40%), Query Frame = 0

Query: 2   TMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T++KFLIV  V IA TSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVG 121
           K+ +DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    G VG
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT---GHVG 122

Query: 122 GFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 181
           GFMYE A+ IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQ
Sbjct: 123 GFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 182

Query: 182 EVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 241
           EVVDCDY+ GGCRGG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERV IDGY
Sbjct: 183 EVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGY 242

Query: 242 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 301
           E VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGS
Sbjct: 243 ENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGS 302

Query: 302 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03270 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.8e-167
Identity = 288/350 (82.29%), Postives = 319/350 (91.14%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           M ++KFLIV +V IAFT HLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FK+ +DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    GRV
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK---NGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDY+  GC GG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERVTIDG
Sbjct: 181 QEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YE VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYG
Sbjct: 241 YENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYG 300

Query: 301 SDEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           +DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 TDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of CSPI04G03270 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.3e-163
Identity = 277/351 (78.92%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT++KFLIV +V +AF+ ++CESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAK--AGGGG 120
           FK+ ++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y +LHAK     GG
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSL 180
           R+GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 240
           SE+EV+DCDY+ GGCRGG YNSAFEF+M N G+TIE+NYPY+ GNGYCRRRG  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 300
           DGYE VP+NNEYALMKAVAHQPVAVA+AS GSDF+FYG GM  E  FCG+ IDHTVVVVG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVG 300

Query: 301 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           YG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 YGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03270 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.4e-161
Identity = 283/352 (80.40%), Postives = 314/352 (89.20%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           M ++KFLIV +V IAFT HLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FK+ +DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    GRV
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK---NGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDY+  GC GG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERVTIDG
Sbjct: 181 QEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRF--YGEGMLREGSFCGYRIDHTVVVVG 300
           YE VP+NNE+ALMKAVAHQPVAVA+ASSG    F  Y +GM  E  FCGY IDHTVVVVG
Sbjct: 241 YENVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNIDHTVVVVG 300

Query: 301 YGSDEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           YG+DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 YGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

BLAST of CSPI04G03270 vs. NCBI nr
Match: XP_031739597.1 (ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529 [Cucumis sativus])

HSP 1 Score: 714.5 bits (1843), Expect = 4.4e-202
Identity = 345/349 (98.85%), Postives = 347/349 (99.43%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTM+KFLIVFVV IAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FKI QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YERVP+NNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG
Sbjct: 241 YERVPRNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 349

BLAST of CSPI04G03270 vs. NCBI nr
Match: XP_031740503.1 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 685.3 bits (1767), Expect = 2.8e-193
Identity = 333/349 (95.42%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTM+KFLIVFVV IAF SHLCE F+LERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FKI QDNAK VF+VNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHY+NLHAKA  GGRV
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKA--GGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYERA  IP SIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYERAMNIPFSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPN+ERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YERVPQNNEYALMKAVAHQPVAV+VASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG
Sbjct: 241 YERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347

BLAST of CSPI04G03270 vs. NCBI nr
Match: XP_031740474.1 (ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cucumis sativus])

HSP 1 Score: 666.0 bits (1717), Expect = 1.8e-187
Identity = 324/349 (92.84%), Postives = 335/349 (95.99%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTM+KFLIVFVV IAFTSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FKI QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHY++LHA      RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHA-----NRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYERA  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSE
Sbjct: 121 GGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDYKVGGCRGG+Y+SAFEFIMQNGGITIEENYPYFAGNGYCRRRGPN+ERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YE VPQNNEYALMKAVAHQPVAV+VASSGSDFRFYGEGMLRE SFCGYRIDHTVVVVGYG
Sbjct: 241 YECVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344

BLAST of CSPI04G03270 vs. NCBI nr
Match: KAE8649111.1 (hypothetical protein Csa_014425 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.4e-184
Identity = 326/352 (92.61%), Postives = 332/352 (94.32%), Query Frame = 0

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTM+KFLIVFVV IAF SHLCE F+LERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FKI QDNAK VF+VNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHY+NLHAKA  GGRV
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKA--GGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYERA  IP SIDWREKGAVNAIKNQGLC       AVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYERAMNIPFSIDWREKGAVNAIKNQGLC-------AVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPN+ERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGE---GMLREGSFCGYRIDHTVVVV 300
           YERVPQNNEYALMKAVAHQPVAV+VASSGSDFRFYGE   GMLREGSFCGYRIDHTVVVV
Sbjct: 241 YERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASVGMLREGSFCGYRIDHTVVVV 300

Query: 301 GYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           GYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 GYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 343

BLAST of CSPI04G03270 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 607.1 bits (1564), Expect = 9.8e-170
Identity = 292/349 (83.67%), Postives = 320/349 (91.69%), Query Frame = 0

Query: 2   TMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T++KFLIV +V IA TSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVG 121
           K+ +DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    G VG
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKT---GHVG 122

Query: 122 GFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 181
           GFMYE A+ IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQ
Sbjct: 123 GFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 182

Query: 182 EVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 241
           EVVDCDY+ GGCRGG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERV IDGY
Sbjct: 183 EVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGY 242

Query: 242 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 301
           E VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGS
Sbjct: 243 ENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGS 302

Query: 302 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03270 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.4e-92
Identity = 177/350 (50.57%), Postives = 233/350 (66.57%), Query Frame = 0

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+ S+      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L     G  +  
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQ-- 120

Query: 123 GFMY--ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLS 182
            FMY  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLS
Sbjct: 121 -FMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLS 180

Query: 183 EQEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 242
           EQE+VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTI
Sbjct: 181 EQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTI 240

Query: 243 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 302
           DG+E VP+N+E AL+KAVA+QPV+VA+ +  SDF+FY EG+   GS CG  ++H V  VG
Sbjct: 241 DGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF-TGS-CGTELNHGVAAVG 300

Query: 303 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           YGS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 YGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03270 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.1e-92
Identity = 170/347 (48.99%), Postives = 232/347 (66.86%), Query Frame = 0

Query: 8   IVFVVSIAFTSHL--CESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKICQ 67
           + F+V I+F S L   + F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF + +
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGFM 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L     G    GGFM
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS---GGFM 122

Query: 128 YERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 187
           YE    +PSS+DWREKGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+V
Sbjct: 123 YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELV 182

Query: 188 DCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYE 247
           DCD +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E
Sbjct: 183 DCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHE 242

Query: 248 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 307
            VP+N+E  L+KAVAHQPV+VA+ +  SDF+ Y EG+      CG +++H VV+VGYG  
Sbjct: 243 HVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYGET 302

Query: 308 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           + G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03270 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 2.9e-87
Identity = 163/348 (46.84%), Postives = 223/348 (64.08%), Query Frame = 0

Query: 4   IKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           +K  IV  + +       +  +   KD ESE SL +LY+RW SHH ++R+  E  KRF +
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  CQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGG 123
            + N KH+   N   KS KL+LN+F D++ +EF   Y GSNI H+       G       
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQ---GEKKATKS 120

Query: 124 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 183
           FMY     +P+S+DWR+ GAV  +KNQG CGSCWAF+ V AVE I+QI+T +L SLSEQE
Sbjct: 121 FMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQE 180

Query: 184 VVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 243
           +VDCD     GC GG  + AFEFI + GG+T E  YPY A +  C     N   V+IDG+
Sbjct: 181 LVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGH 240

Query: 244 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 303
           E VP+N+E  LMKAVA+QPV+VA+ + GSDF+FY EG+      CG  ++H V VVGYG+
Sbjct: 241 EDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFT--GRCGTELNHGVAVVGYGT 300

Query: 304 DEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
             +G  YWI++N +G +WG  GY++MQRG R+ +G+CG+AM+ S+P+K
Sbjct: 301 TIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CSPI04G03270 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 282.3 bits (721), Expect = 5.2e-76
Identity = 147/343 (42.86%), Postives = 212/343 (61.81%), Query Frame = 0

Query: 12  VSIAF-TSHLCESFELERKDFESEKSLMQLYKRW-SSHHRISRNAHEMHKRFKICQDNAK 71
           +S++F +SH         +D ES   L++L++ W S+  +      E   RF++ +DN K
Sbjct: 21  LSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLK 80

Query: 72  HVFRVNHMGKSLKLRLNQFADLSDDEFSMMY---GSNITHYDNLHAKAGGGGRVGGFMYE 131
           H+   N  GKS  L LN+FADLS +EF  MY    ++I   D   + A        F Y 
Sbjct: 81  HIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRDEERSYA-------EFAYR 140

Query: 132 RARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDC 191
               +P S+DWR+KGAV  +KNQG CGSCWAF+ VAAVE I++I T  L +LSEQE++DC
Sbjct: 141 DVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDC 200

Query: 192 DYKV-GGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERVP 251
           D     GC GG  + AFE+I++NGG+  EE+YPY    G C  +   +E VTI+G++ VP
Sbjct: 201 DTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVP 260

Query: 252 QNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSDEEG 311
            N+E +L+KA+AHQP++VA+ +SG +F+FY  G+      CG  +DH V  VGYGS +  
Sbjct: 261 TNDEKSLLKALAHQPLSVAIDASGREFQFYSGGVF--DGRCGVDLDHGVAAVGYGSSKGS 320

Query: 312 DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           DY I++N +G +WG  GY++++R T  P+G+CG+    SFP K
Sbjct: 321 DYIIVKNSWGPKWGEKGYIRLKRNTGKPEGLCGINKMASFPTK 354

BLAST of CSPI04G03270 vs. TAIR 10
Match: AT3G19400.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.8e-74
Identity = 156/347 (44.96%), Postives = 217/347 (62.54%), Query Frame = 0

Query: 8   IVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA-HEMHKRFKICQD 67
           +V +  +  +S L  + E E +  E+E  LM  Y++W   +R + N   E  +RFKI +D
Sbjct: 13  LVILSVLLLSSSLGVATETEIERNETEVRLM--YEQWLVENRKNYNGLGEKERRFKIFKD 72

Query: 68  NAKHVFRVNHM-GKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVGGFMY 127
           N K V   N +  ++ ++ L +FADL+++EF  +Y            +     +   ++Y
Sbjct: 73  NLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIY------LRKKMERTKDSVKTERYLY 132

Query: 128 ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 187
           +    +P  +DWR  GAV ++K+QG CGSCWAF+AV AVE I+QI T EL+SLSEQE+VD
Sbjct: 133 KEGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVD 192

Query: 188 CD--YKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNER-VTIDGY 247
           CD  +   GC GG  N AFEFIM+NGGI  +++YPY A + G C     NN R VTIDGY
Sbjct: 193 CDRGFVNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTIDGY 252

Query: 248 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 307
           E VP+++E +L KAVAHQPV+VA+ +S   F+ Y  G++     CG  +DH VVVVGYGS
Sbjct: 253 EDVPRDDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMT--GTCGISLDHGVVVVGYGS 312

Query: 308 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
               DYWIIRN +G  WG +GY+K+QR   +P G CG+AM PS+P K
Sbjct: 313 TSGEDYWIIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTK 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O650396.3e-9551.43Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
P124124.1e-9449.71Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
Q9STL41.9e-9150.57KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
P258032.5e-9149.86Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9STL57.2e-9148.99KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ... [more]
Match NameE-valueIdentityDescription
A0A1S3BYQ34.8e-17083.67ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1[more]
A0A5A7TM648.1e-17083.67Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034... [more]
A0A1S3BYU03.8e-16782.29ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1[more]
A0A0A0KGB14.3e-16378.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1[more]
A0A5D3D0431.4e-16180.40Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001... [more]
Match NameE-valueIdentityDescription
XP_031739597.14.4e-20298.85ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529... [more]
XP_031740503.12.8e-19395.42ervatamin-B [Cucumis sativus][more]
XP_031740474.11.8e-18792.84ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cuc... [more]
KAE8649111.11.4e-18492.61hypothetical protein Csa_014425 [Cucumis sativus][more]
XP_008454483.19.8e-17083.67PREDICTED: ervatamin-B-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G48340.11.4e-9250.57Cysteine proteinases superfamily protein [more]
AT3G48350.15.1e-9248.99Cysteine proteinases superfamily protein [more]
AT5G50260.12.9e-8746.84Cysteine proteinases superfamily protein [more]
AT1G20850.15.2e-7642.86xylem cysteine peptidase 2 [more]
AT3G19400.14.8e-7444.96Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 292..302
score: 52.4
coord: 149..164
score: 62.4
coord: 307..313
score: 55.51
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 131..347
e-value: 1.3E-96
score: 336.9
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 131..346
e-value: 2.5E-71
score: 240.2
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..96
e-value: 1.2E-5
score: 34.7
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..96
e-value: 8.1E-8
score: 32.6
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 13..349
e-value: 1.8E-102
score: 345.4
NoneNo IPR availablePANTHERPTHR12411:SF706KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1coord: 21..348
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 21..348
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 149..160
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 132..346
e-value: 9.44297E-96
score: 281.435
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 37..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03270.1CSPI04G03270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0004713 protein tyrosine kinase activity
molecular_function GO:0008234 cysteine-type peptidase activity