CSPI04G03200 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G03200
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionIsoleucyl-tRNA synthetase
LocationChr4: 1983320 .. 2003243 (+)
RNA-Seq ExpressionCSPI04G03200
SyntenyCSPI04G03200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAACATAATTTCCTAAGGTAAAAAGGTACATTTTTTGGTGTTATTATTTAATTTTTTTGGGTGAAAGAAATGACTTTGATCTTTTAGGTTTACAGCAATTCTCTCTCAGCTGCTCCCTCCATCTCTCCTATATTCCTATTTCTACAGCCACTGCCACCCCACTCCGTCGCCCGCCGTCGCTCACCACACCCTCCAGGTCTCCACTCTAATCTAACCGGTTCAGCCTCCCGACTAAATCGCCGCCGCCGCAATCGGGGTTAACTAAACCGGTTATTTAATTACCTTAAAACTGAGCTGAGCAGACCAGACCAGACCAACGCAGCTGATTTAGAAACGCACTTTCAGTCCTTCAATTTCACTTCTTCAAATCAGCCGCTGACGATAACTACTAGGGTATTTCTCTACATTTTCTCATTTCAACTCTCCAAGATTGTCACTCAGAATTCCGAGCTCAGTATCATCTTCTTCATTTCCTTTTGTTTTGTTTTGTTTTGTTTTTTTATTTCTTTAAATTTCTTGCTGCAAAGTGTTTGAATGTTTTGCCTCACAAGTCAACATCTTTATGCAGGTTGAATTGTGGGGACTTCTTGTTATTATCCAGAATATAGTTTTTATACTCTAATTGAACAAACACCCTTTTCTTCAACTTAGGTGTAGTGCCGCCATGGATGAAGTTTGCGAGGGTAAGGACTTTTCGTTCCCTAAGCATGAGGAGGGGGTGCTTGAGTATTGGTCTGATATCAAGGCATTTGAGACTCAGCTCGAGCGCACACGGGACCTTCCTGAGTATATTTTTTATGATGGGCCTCCATTCGCCACTGGTCTTCCACATTATGGCCACATATTGGCGGGGACAATCAAGGACATTGTCACCCGGTATCAGGTTATGACCGGTCATCATGTGACTCGCCGCTTTGGATGGGATTGTCATGGATTGCCTGTTGAGAATGAGATTGATCAGAAGCTGGGTATTAAGAGGAGAGACGATGTGTTGAAGATGGGGATTGACAAGTACAACGAAGAATGCAGAAGTATAGTTACGCGGTATGTGGGGGAGTGGGAAAAAATCATCACGCGTACTGGACGATGGATTGATTTTAAGAACGACTATAAGACAATGGACTTGAAGTTCATGGAGAGTGTTTGGTGGGTTTTCTCTCAGCTCTACCAGAAAGGTCTTGTCTATAAAGGTTTTAAGGTTAGTTGGTTTATAATCTTTCTTTATTTGCATATTTCGGCTAATATTCATCGACTCCTTTTATTATACCATCAGTTGCTAGTTCATTCTTTCACGACATTTTCCCCCTTAAAACATGAGAATAGGGGGTGGAAAGCCATGTGAAATTTAACACCTGTGGGAAATTCAGTTTGTTTGATGCGATATAGGATTTGAAGGTTGCCTTTAATTTTCTTGACTCACTGTGTTATATTTTTTAGTTTTCATTTGATCATCAAAGGATATATGCACGATGGGATCATCATCATTTGGAGCTAAGTAGCTGAGTATATTCTTTCATTGGAGATCATCATTGAGTCGTTAGTTACTTCTCAAAATTTGAATAAAAATATGCTACGGATAATTTTTCTTTTTAAATTTAGAGGGAGAAAATACGTGCTTGGGTCATATTGCATTGCACTGCATTGGATTCTGTGATCTGAGGATGTTCTTTATCCAGTTTATTTGATGATAATGTCATGTTATGGTATCTTTATGTGTAGAAACTTTGAAAATAAATGTCTTAGGTAGCTCACTTGGGCATGAGTCCTTATATTTGACATATAGTTTGTCAACCCAGGAGTTGTTTTAGGTCTCTTCTCTACTTGTGCTTCATAACAATCTTTAATCATAAGCGCTAGACAACTAACGAGAGTATAGTTAAAGATTTTGACCACATTTTGTTGTTGATGGACATTTTTTATTTAGTGAAGGCAATATCATACCTAGCCATGATTATTAAGGGTGTAATAATATGTAGTAGGTTTGTGGCATCCATTACGATATCATTTGTAATATTAGTCAATAGATGAAATAATATTACCTTTAACTTACTGTTGATTTGTTACCTTTCTTATCTCAAACCTATCTCAGTTGGAGTGAGGTTCAATCTTTAATCCAAAATCTTTTACCAATATTGGGGACAGTACATCCTTTCAACTTGTTGAAAATGGATTACTACTTATTGGCTTTCACCTTGTCCTAAATATGACACACCTTTGTGTACAATTAGGACAGGGTTTTGGCATTAGCCAAAAAGTCTTTGTTCTCTCTTTGACCAAGAAAAGAGAAAATTTCAATGAAGTAATGGAATGAGACCAATGCTCAGAAAATCTGATCTCCATCGAGGAATAAAAAAGATGGGTGGGGAAGAATTCCCTTTGTACTGTTTTCCACTTCTGCATCATCTTTCCTCATCCGAAAATTGTACTATCTCGGATAATTTGGTGAGATATGAGAATTTTGTGTCTTTCTCTTTTGGGTTCTATCGTAACTTGACCAAACAACAAAGGTGGCCTCTCTTCTTTCCTTACTCGAGGAGTGCACTATTAAAGAGGGGAGGAGAGAGGCTCGTGTTTGGAATCCTAATCCTAGTTGATGTTTTTCTTGTAAATCTCTGTTTTGTTTGTTGTTGGATCTCGCTCCCCCTAAGGAGTTGGTCTTTGATGTGGTGTGGAGGACTAAGGTTCCTAAGAAAGTTAGGTTCTTTATCCGGCAAGTCTTGTTTGGCTGGGTTAACACTATTGATAGGCTTGTCGGGAGGAAGACTTCGCTTGTCGGGCCTTTATGTTGTATGTTGTGTCTAATGACGAAGGAAGATCTTGATCACCTTTTTTGGGATTGCCAGCTTGTAAGGGTCGTGTAGTGCTCCTTTCTTTAGGAGTTCGGTGTTAGCTTTGCCAACTTGAGGAGTGTTGGAGTGACGATTGAGGAGTTCCTTCTCCATCCGCTCTTCAGTGATAAGGGAGGCTTTTTTATGGTGTGCTGTGGTATGGTTATTTGGGATATTTGGGGCGAGAGAATTCTAGGGTGTTTAGGAGTAGAGAGAGGGAGCATAGTGAGATTTGGTCTTTGGTTAGATTTTACGTGTCTCTTTGGGCTTCGGTTTCGAAGAACTATTGTAACTATTCTATAGACAACATTTTACTTATTTGGTCCCCCTTCCTTTAAAAGGATGGTTTTGTGGGCTGATTTTTTTGTATGTCCTTGTATTCTTTCATTCTTTCTCAATGAAAGTTATCGTTTTCATCAAAGAAGGAAAAAAAAAAAAAAAAAAACTAAGAAGGAAAAAGAAAAGTTGTAAAATACAGACTTCTTGTTCCTCTATCTCCTTCAAAATATGCGTCTTCTTTAGAAAATTCTTCCTTTTCATTTTCATGCTTGTGTGTAGTTTGCTTCTTATATTCTAGTGACTTGCTTTCTTTCTCAGAAGTTTTTTGATAAATGACCCATGTCATCATCAACCACAACTACAAAATTACCCTTTCTCTCTTCCCTATTCTCTTTTTTCTTTTCTTCTTCTTCTTCCCTTCTGTTTCTACCTTTGATTTTCTTTTTCTCCCTTCCTCTCCTCTGTTCCAACCTCCAAATTTCCCCCTCCCTTATCTCTTATGGGTTTTTATTCTTTGTGACTTTTCCCCCTTGTTCCTGCATTCATAGTTTTGGTTTATGTTGTTGAGTGTAAGAGTGACCGGCCAATCTGTGTGAAAGGAGAGAGTGAGGAGAATAAGGCAAGAGAGAATGACATAGAGAGGGGAGATTTCAGTGAGAGTGAGACAAAGAGAGGGAGAAGCTCAATCTATGTGCTTGAGGTTATGTTTTGGTAATTTCACCCAACTCGATTCCCCCCCCCCAATACCGAGTTCTTCATCCATTTTCCCTTTTGTGAGAGGGGTTGTTTCTTATGGTTGACAGAGGTGTGTGAGCACATGGAGAGAAGAACAATAGGGCCATCAATACCAAATCCAACAGCCCCATACATAAATGATTTTGATAAAGACCCTTCTCTAAGCAAATCTTTTGTTTTTTGTCTTATGAAATAGAGCTTTCTAGATTTCGTAGTTATAGTTGTAGTTATCGTGTAGGTTTATGCTTTTCTGTTTCTTTCTTGTTTTGCCATGTTTAACTAACATTCGTTATTTATTTGTTTTCCTGACTGCTTTAAAGTGGTTTACATGGTACCGAACGATGTATATATCTTTTAATTATTGTTAATCCTTGGAAAAGCTATGTAAATATAACTAACTGCAATTAATCTAATTCAACTACTGAGTAGGAGTTCAATTATAAATTTGTTTGCATTGTTTTTTTCTTAGGTCATGCCATATAGTACTGGTTGCAAGACACCACTTTCAAACTTCGAAGCTGGCCAGAGCTACAAGGTTTGCAACTCTTTCTTCAATTGAAATTTTTTCCTTACATTAAATATAGTTGAATCACCTTTGTATGTAGAAGCCATGGAATGCATGGTGTAGTTGCTATTTCCTGAGAAATTTTGAATATTCAATAAATCAAGAATACAATATATGACATATTTGGTTAATTAAGAATACGTAATTCGAAATACAAAAGCTACTTTTTTTTAAAGTAAACCTATTTTCTTTCATTTTCTTACAAGGATTTGCTTATTTCTTAATTAAAAGAGTTGAACTCTTAGGCAAATCTAAAATTTTTTGCTTAATTTTGTGCAAGGTTTGATTTTTGTTGATTTTTTTACCCAAAGTATGAATCTATGATGGTTTTTTTCATTTTCTACAATGTAAGAAAATTATCACGTGAGCTTTTAGTTTGTCAGTATTGTAAGAAAATAATAGTTAAAATTTGCAAAGTGATTCTTCTTCTTTTTTGATAGTAAATTGTATTTAAGTGCATTATTTTTTTTAGTGGGCAAATAACAACTTAGTAAGCACGATAATTTGTACATTAAATAGGATTGGGATGGGTTCAAAGAAAAAGCCAAATTTTTGTTCCGTGTTCATTTGGTGTATGTAGCATAGATTTTCAAATCACGTCAAGAGAATTTTCAGTCTTAGTCTTTGGTGGTTTGTCTACATAAAATTTCATGTTTTCCAATTGCATTATTAGACACCAGCTAATGTATGAATTGTTGATTTGCATCAGATTGACTTATAATCTGTCTTCTGATATCCAAGAGTATTACCTTACACAGGATGTCCCTGATCCTGAAATAATGGTAGCTTTTCCAGTACTTGGGGATCCCCAAAATGCGGCTTTTGTGGCTTGGACAACTACTCCGTGGACCCTTCCGAGTAATCTTGCCCTTTGTGTTAATGCCAATTTTGTTTATGTGAAGGTCCGATTATGTAAATTTATGTTCAACATATTGCATTTTGGTAGTAACTAGCAAATTTAAGTATACTTTTATGTGGTCTTAAACTCTTTTCACCAGATTTTTTTTACTGTTTGCTGTTCTTTTTGACTTTACAATTTAACCTTGTCAAATCTCTTTGTGAAGTGCATGCAGATGAATTTTATTTTGCTGCATCTTTGGTTAAGAAATGACCTTTGAAATTTGTCTTCCCTGTTTGATCCTTGAATATATTAGCCATCATCCCTATCTTCAGTTAGGCACCACCAACTACTAGGTACGTGTATGGTTAGGTCGTTTGTGGTTTTTCCTTTTCTTGATCTATACAGAGGTCCAGGAATAAATTCAGCTTTGTTACACATCTTTTGGAAATGCCTTCAATCTGGTCAGAAGACTCGAGATGAAAATATCAGTGTACAACATCTACACTCGGCATCAATCCCCATCAAGAAGCTCTGATCAACCGCGCCATAAAGCTCTTCAAGCTGAATGGAGCCTTCCTTTTTTTTCTTTCCTACTTCTTTTGCGTAATTTTTGTGTGCTCTTGTATTCCTTCATTAGTTTCAATGAAAGTTTAATTTCTTTCCGGAAAAGTTTGTGCAGAAAAGAGCCTCCATCCTGTGGCCATTGTCTTCCTAGGTTCTTTGTTTGTGCCATCTTTCACTCAAGAAACACTATCCTGTGGCATTTGTGTTGCCTTCAAGTCCTCTGTTTCTCTTTAGGCCTTTGTTTCTAGACTCTTTTGTAATTGTTTGGCAGGTCTTATTTCTCTTAATAGGAGTCCTTTCTTGTTCTTTGCTTTCTTTCTGTGGGCTTTTTTTTTTCCTGAATGAGAGTCGGGGCTTTTCATTAAAAAAATTCATAATAATATAAGTGTATCAACATCACACAGGCTCCCGGTTAAATCTTTTATGCTCAATTGTATTTAGTTTTTCATTCATTTATACACAAACTGTATATTTTTCGACATATTGCGAATCTTACAGCATTTAGATGTTTTGTTCATTGTGCCTAAGCTCTCAGAAGTTCTTTTTATAGTTGATAATCTAATGTAATAGAAAAAAAATACCAGGTTCGCAACAAGGTGTCCGGAAAAGTATACGTGGTTGCTAAATCTCGATTGTCTGCTTTACCTGTCGAGAAACAAAAAGTGAATGCAGCAAATGCTAATGATGGCATTAACAAGTCAAATCCTAAGAAAAAGGGATCCTCGAATTCAAAAACAGAGAACTCAGTCGAGGACTCATTCGAGATATTGGAGGAAGTATTAGGAGCTTCTCTTGTGGGTAAAAAGTAAGCCTTTTCATTGGTTGATACGTTTCTTGATGATTTTAGCAAGGCTGTTATATACATACATATATGCATGCCTGTATGTGTATATGTGTGCGTGTGTGTGTGTGTTTTTTTTTCTGGTTCCTGAATGTTTATTAAATTGTATCTAATGATATTGCTGAAGGATATTAAATATATTGTATTTCCAGGTACGTGCCATTGTTTGATTACTTCAAAGAGTTTTCTGATGTGGCATTTAGAGTTGTTGCGGATAGTTATGTCACTGATGATAGTGGTACGGGCATTGTCCATTGTGCTCCTGCATTTGGTGAAGATGATTATCGTGTTTGCATTGAGAACCAAGTGATCAATAAGGTTTGTCATGAATATTCTTTTATTCTTCACATATCATTAGTAAGTTTTATATGACTTCACAGCACATGATTAGTGACAACCAAGAAAAGTGATGTGGAATAACTTGTTAAGTATTGGTGTTTTGAAGGTCAGTGATCTGAGGTGCATTTGGTTAGTAAAGGTGATCGAGAAACAGAAGTTTAAAACTAACACTCCAAATGATTACTATTTGGTTTTTAGTCTACTAGTAGTGTGTGTTCTCTCACCATTTCTTTCAAGTGGTTCTTGTTTACCATGTAACCATTTGAGTTCTTTGCCAAATTATAATAATAAAATCAAGTTTTATAAAACTATCTTTGGAAGTTTTTTAAACCTTATTTGAATTTTGAAACACTTGTAAAAGGTAGATAACAAAGTAAAAAAACCAATAGGTGGAAGTAGTGTTTATACCTTAATTTTCAAAAACTGAAAACAAAAAAAAATCAATGGTTATCATATCAAATGGGACCTAAGAGATTCAATGAAAAAACAAAGTTGTATTTTTAAATATACTTTTGATAGGTTATGAATTTTAAAAGTTGTTCAAAACGTTTGGTGATATGTTTATAAATAATAAGAACAGTAGTAGTTACTAACTAATAATTTAACTAAATCTCAAATATTAATGATTACTATATTAATATATTTAATGTTATGAATTCTTGTATCTACCAACTAATGTTATTAATTAATTTTTAAAATAGTTACCAACTAATAATTATTGTTCGATTATTGTTTTCGAATAAATATTATCTGTTATATTATGTCATACATGTTTCGATTTTTGCAAGAGTTAAAAGGAGTTTGTCAAATTGAATAATATTTTTTTATCAGATTTTTGGTTTTTTTTTTTTTTTTTAATGTAATGCAAAATACTTCTACAAATTCAAACGTTGTTTTCAAAGTTTGGAGGGAAATATTCAACATTCTTATTTTCAAAACATCTCATTTGTATTCTCTCTCATTTACTTGCTAATTATTCCTTTAAAGAAATTTGATCAAAGTTGGAGGCCTTTTTTCTCCTCGCTAGATATTTAAGTTCAAGTTGACTTATGTTGTTTATTTTCATTTTCTGCTTGACCCCTTCTGTTTCTTTGCATAACTGAAATGAGAATGCCGTATCTTGTTTAAAATTAAAAGATCACTAAAGTGCACTATAATTGTTTGTGAGAATAAGTTACTCTACATTTCTGTCTTATTTTTGCTCGTGTTTTTTCCATTTGCTTGTAGGGTGAGAATTTGATTGTTGCAGTCGATGATGATGGTTGCTTTATTTCAAAGATTACCGATTTTTCTGGACGTTATGTCAAGGATGCAGACAAGGATATAATTGAGGCGGTGAAGGTATGTTCTTGCCCTTGTAGTAGTATGTTCAAGTTGAATTATTTTTTCTGGGAATATCTATCGTTTCATCATGTAGATATAGCATCCCAATGTATTCATGTGTGATATGAAAACCCATGTAAATTTTTAACTCTTTGATGCAATCTCAATAAGCTTGGAGAATTCAAAGTGGAGCTTGAATTCACAATTTGGCCTTGTTTCTTGTTTGCAACTTGAAGTAAGCTTTTGATTGAGAAACAAACTTTGAATAGAATAACGTTTGGATCTTGAACTTGTAATTTCAACTATGGGTGAATGTGAATCTTCATTAGCTTTATCCTTGAAGACATATCTACTTGACGATTACGTTTTCATTCTGTAATTTTATATTTATGGTGATCTTCTTGTTGCTTTTGGACTAGGGAAGTATAAAGGAGAGGAGGAAAACAACCTATTTGAAATAAAAATATGTTGGAATCTAAAATTCTTTATTATTTCATTATGAACGTGCATTATTGGTCAGAAATTTCCTGTTTCAGGCACAAGGAAGGCTTGTTAAATCTGGAAGCTTTACCCATTCTTATCCTTTCTGCTGGAGATCTGATACTCCCCTTATCTACAGGGCTGTTCCAAGCTGGCAAGTCCATTTATTATCTATTTGAAATTTGCTATTTTCTTTCATGATAAGGTCCTCCTTTTCACTTTGGTAATTTTGTTTTTGCGTATGTGTACATTTTGACACTGCAACTGTTTGCCTCCTGCCCTCTGGATAAATGAAGAGTTCAGTTAGGTTTGGTTCACACTTTGGGCGCAGTTGCGAAGAGAGTATTTTTAAAATGGCAAAGAAATTATGGAATATTCCTTTTATAGCTTTTTCTTTAGAAAGAAAACCCTCGTAATACTATTACTGAATTCTAAATTCACACTTGATTTTAGTGCTTATATTGCTCATTTTCTCTTCCTGTTGCACATTCCACCTTTGTCTTATCTGATATTCATTTTTGAATGCGGACAAATGTAAATAAACTTGTTTTTTGTTTGTTTGTCTTCCTCCTACTGTTACTATCCTAACCAATCGGACTTCTCTTACTTTAGGTTTGTCCGAGTTGAGAAATTAAAGGAGAATTTACTAGAAAACAATGAGAAGACTTACTGGGTGCCTGACTTTGTGAAGGTTTTATATTTTATGCTGAACTCCTCTCTTAAATACTCACATTTCACTTTATTTGAATTTTGGTCAAGTATGCATGGGCAAGGTTGAATGGGGTATAGTATTATGCACGGTGTAAAAAGTAGTAGTGTGATATGAATCAAGATATATAAGATTAATAGAATTTTATAAAAAAGATTGATAGAAGTTTTTTGTTGTATCTATGCTTCTGTGGGCTTTTGTGGCAAATCCTTCTTTTACTTATTGTTAGAAACTTGAAACTTGTTTTCCTTGGTTGGACAGCTTTTCTGTAGTTTCACTTCTTTTATCTGGTCTCTCTTTTTGTATTCTTTCGTTTCTTTTCAATTAAATTCTCGTGCATTGATATAACTATGCTTCTGAGAAAGAGAACCATCATGAAAATCTTCCATGTTTAAAATAAACAACTATAAACTCTCAAGAGTTGAATCGTTTATATATATATATATATATATATATATTCTTTTCATTGCTTGAAGATACAAACATTTTAGAACTATGAAACTTGAGTCCTGAAATATACTTGGGTTGAAAAAACAAAGTGCTGATGTGAAAATGAACTGTTTCATAGCTCGTGAGATCTATTTTGTCTTCATCTTTATTTGTTAATGTCAAATTGTAGGAGAAAAGGTTCCATAATTGGCTAGAGAATGCAAGAGATTGGGCTGTCAGTCGTAGCAGATTTTGGGGAACACCTCTCCCTGTCTGGGTTAGCGAGGATGGGGAGGAAATACTGGTCATGGACTCAATTGAGAAACTTGAAAAGCTTTCTGGTGTTAAGGTATCACTCTTATTTCCCGGTTTTTGTTATCTTCAAATATAACTGCTTTAGGTAGAGGCAGCCATTTTTTCTCTTTATCGAATCGTTTGCTATGTATGGATTTTTTTAAGCTTGCCATAGGAACTTAAATCGGTTTACCGTGAGTTTTATTTCTCTTCAAACTGTCTGGTTTCCATGCAGAGGGGTGAGGACATGATTCATACCGTAGAGAAGAAAATAAATCGACATCTTCTAACGTAAAAAAAATTATGAAATGGCTTGGTGACCATCATGTATTCTTTAAGCAGTTAATTCTTATGTTCTGGTGGGGACTGCTTGTAATCTACTTGTGAGTTATCTGTGGGGATCTTATGTTTTCGAGCTTTCAGGTGTTTGATCTGCATCGTCACAAGATCGATCACATTACTATTCCATCCAGAAGAGGTCCTGAATATGGTGTTCTTCGGAGGGTAGATGATGTAAGACTACTTAATCTCGTTTTTCTCTTAAATATTATTTTTCTGGATTAGAAATTGATCAATACTGGCGTTTTCTTGCACTTTCTCTGGAACTATGGAAGTTAGGATAATTTTTTTTTTTTGCCTTTTTCTGCTTCACACTTTTATTTTATTTTTGGATTCAATCACTTACTTTTGATACTACTCTTTTGTAATGGATCTTGTAACTTCAAAATTTTCTCACATAGATAGATAATTAGAGGTCACCACCGTTGATTATTTAGACTAGAGGGAATAAGAAGAGGAGGGTAAGGGGTATAAAGGTAATCTACATGGCGGAGGATGGGGAATTAGTAAACATGGCCAAGGGTGTGATGAGGCATTATTGTGACTTGGGTGAAAATCCTTTGGCATCCTTTTGCCATTATATGGAGGATTTGGCTTCCGAGAAAAATTGTGAAATTTGTGTTGTTAGTTAGAGAGTGAGAGGTCTGTGGGAGGCGATTAGACCTCATGCCTTGGTTTGAGCATACCTGGCTAAGGATTTCTATAATCATCCTTTTGGATTGGAGTCATTTTCTTCGTTCGATGGGCTCCATCTGTTAGCCTTTTGCTTTTATGCTTCTGATTCTTTTATTTTTCTTTTCAATGAAAGAAAAATAAGTAAAAAATAGTACACAAATACGTGCAATCTATTTGAGTGAGTTGCTGTAGTTTCCCTCTCTTAACCATGTTTTCTACCTGTATATTTTTTAATGAAAAATTTATTTCCTGGTGTTCATACTTGCAGTATATTTTGTTCCCTCATCTTTAACAGGTGTTTGATTGCTGGTTTGAGAGTGGGTCTATGCCATATGCTTACATTCATTATCCATTCGAGAACGTTGAGCTTTTTGAGAAGAATTTTCCTGGGCACTTTGTGGCAGAGGGGCTTGATCAAACTCGTGGATGGTAAGTTTCTTGGTGTTTGTGTCTTTTATTTTTTTTTACCCTTCATAGTTCCAAAATATCAACGAGTCAAGTTGCAAAATTATTTTCCTGTTACTTTGGAGTTGTAAATCATTAGTGTTTTTCTTACAATAATTGTAGGGGTAGTCGATCAAACCCCTCACCTTTAAGATGAAAGGTTATGCCAATTACCGTTGAGCTAAGCTCACTTTGGCAGTTGTAAATCACTAGTTGGATTGTATTTAGTGGTGAAAAACTCAAATATATGCACGAGAGTGGAATGTTGATATAATTCTCTTTTAAATTGATGCTTCTTTTTCTTTTTTGGTTGGATAATTGATAACTTATTTTATTGATATGAGGAAGCAGACACAATATGAGCATGGTGTGAAACAATGACTTTTGGTGATTAATAATCTACTACATTATTTTTTCATGTTTTGAACACATTGAAAATCTACTACATTTTTTTTTTCATTTTTTGAACACATTGAAAGGAACCAAAACTTCATTAATATAATGAAAAGAGACTAATGTTCATTTTTGATGAGATATAGATTGTTTGCTCTTTACGTCTGCAGGTTCTACACTCTCATGGTTCTATCTACTGCTTTATTTGGGAAGCCTGCATTTAGGAACCTTATTTGTAATGGCCTTGTGCTTGCTGAGGATGGTAAGAAGATGAGTAAAAGGTTGAAAAACTATCCTTCACCAATGGAAATCATAAATGATTATGGAGCTGTAAGTTCTGTATTATATCTATGTTTTTCACTTATCTTGTATTTCTGACTTCACTTTTCATTATTCTGATGACAATGACATTGAAAAACAAGATTGGTGTAAATATACAAACTCCCTTAAAGACTATAGTTTCAAAAGCCTCATTGCTAGGTGTTCTTGTATTATACCTTGGATGGTTGGAAGGCTCTTCCTTCCTTTGAGTACAATTTCGTTCAATCAATGAAATTATGTTTATTCTTCTTCCAAAAAGAGTTAATAATAATAATCATAAATAAAAAAGCAAGCATCAAGATATTCATTGAGCTTTCTTTTCAATTAATCGTGGTTATTATTTTATGAAGGTTGGTGTGTGTTTTTTTTGTTGCAGGATGCTCTGAGATTATATCTTATAAATTCTCCAGTTGTACGTGCAGAGACTTTGCGATTCAAGAAGGAGGGTGTCTTTGGTGTTGTAAGTCTTTACTGATATTTTACACTGTACCAAGTATTCTCGGTGCACCACTTGGGCACCATGTCTTCGTTAACTTTAACTTTACTTCATAGTTCATGAACAGCTATACTGAGACCTGTTTTTCCTCACTTTGACTTTATGGACTGTTGACAGGTCAGGGATGTATTCCTGCCATGGTACAATGCATATAGATTCCTTGTTCAGAATGCCAAGAGGCTCGAGATTGAGGGGTTTGCACCATTTTCTCCAGTGGATCAGGCCACCCTTCAAAAATCATTCAATGTGCTTGACCAATGGATAAACTCTGCCACCCAGAGTTTAGTATATTTTGTCAGGAAAGAAATGGATGGCTATCGACTCTATACGGTTCGGCACTTTCAATTGATATATATGGCTCCTATCTTCTCTTGGATATGATTGATTGACTTTCTGATTATATATCTTTGTGATTTTATTAGAAAATTAAGGCCTATGCATACACCTCAATACTTGTTTTAGATTCCTGTTAGCAGCTGTGAAAGTTTTTGTTCCTTTCTTTTCTGTTTTTCTTTTAATTTTTTTTTTCTTGAAACTTAATTGCAATTGTTGGACTCTCTTTTGTTCGACATTTCTCATCATTAACCGTGCTCTCAATACAACAGGTTGTTCCATATCTTCTAAAGTTCCTTGACAACCTTACAAATATTTATGTGAGGTTCAACCGCAAGAGACTAAAAGGCCGTACTGGGGAAGAAGATTGCAGGATAGCACTCTCTACTCTGTACCATGTATGGTGCATTTTCCCCCGCTATTTAGATAAATGATTGACAATTTTTTTAACTGTCAACTTTTTGGTAGTGTACAAATCTGCGTATGTGGTTTATTAACTTCTCTGTTTCTGTATTGCTGTAAATTAAGTCGTAAGGGCGAGTGACTCATCTTATTAAAGACAGACTAAAGCTGCTCACTCTGAGAAATTTAATTGATATAAGCTGAATTTTGGTCATTTTTCAGTCGACAAATTAGTCAGACAGAGACATTGATGATTCATTTCCCTTTATTTGGGGATAGAGTTTGAGTGCTATTAATTAACAATCTAAATTGTAGAGTTCGTTCTTTCTTATATATAGATTACACCTATATTATGGTTATAAGATGTTTCGTTGACATACTTGGCTTTTTTATTTTTGTTCATATCGGTGTACTATGTTGTATATTTACATAGTGGAGTATTATAGTTCTTTCAATTTTATGGGAAGTGTTATTATCATTTAAATGAATATCTTGTTTAATTTTTTTTTTTTTTTGGTTAATGCAAATTGTTTCTGCTTCATGTGAATTAATGTTGAATCACACCACTTCGAATGTAGTATTATATTCTGTGTTTTTCTTCTTAGCTGTCAAATCTGTTATTTCCTTGTTCATTTTATTTTCTATTTTGATCTGAGCACGGAGTATATTGTGCCTTCAGGTCCTTTTAACATCTTGTAAGGTCATGGCTCCATTTACTCCATTTTTCACTGAAGTTTTATATCAAAACTTGCGGAAAGTTTCTGATGGGTCAGAGGAAAGCATTCATTACTGTTCTTATCCTCAAGAAGGAGGAAAGGTATTTATTATCGGTTTACTTATATGCTATAATATTTGTAATCATTTTTTGAAATTATTCCTGTGTAGCAAGGGGCACTGTATTTTGTGTTTTGTTGTCAAAGAATTGAGAAGCACTTTTGTAACACCTTTAATTTTTTTGAATATTTATTTATTTATTTTAAATGAGAGCCTTTTCCTACTTCAAGGGGTTGGATTGTTTGAACACAAATTCCCTTCTTCATAGTTTGTTTTACATTTTAATGAAAATGTCTGTTAAGTGACACTATGATGTACTTATGATTTAATATCCTATGAGTTTTCTTGAAACCTAAATGCTGTTGAGTCAAGTATGTTGTCTCGTGAGATCAGTTGAGGTGAGCGTAATCTGTCCCACAAACTAACGAATATAAAAGAAAATGATACAACATTATGAAATCTATTAAAATAACTATTAATGTTAAGCTCTTCACATACACTTTAGAAATCATAAACTGAAAAGGGTCTTTGAGCCTCTCTTTTTTATCTTTGAATTTTATTTGTAAAAAGATACATGTCAGCAGAAAGCTTACCCCCTACCTTGAGCTGAATTTGAATTCCTCACGAATGAGTTTTTTTATAACTTCATCCAACTTGATTTCTTATGACTACTGCTTGTTGATGATTATTAACCATTGGCAGACTTTTTTGTAATAGTTTTTTGGGAGGGGCTCCCCTCTACCCCCGGCCCCTAGGCCGTATTTTTTTCTCACTTTGAATATATGCCTGTTGATGTTTCTTATAAAAAGTCCAACTTGATTTCTTACATTTGTTTCACTTTATGATATTTGATATGCCTTATTGAATGCCAACTTAGAGAGGGGAAAGAATTGAAGAAAGTGTTACAAGGATGATGACAATTATTGATCTTGCTCGCAACATTCGGGAGCGTCATAACAAACCTCTTAAAGCGCCTTTGAGGTTTGTAGATGCATATATTTTTGCTTCTTGCTGATTTTATTGTAGCATTTTCCATGCTCATATCTAATTCTGTAATCTCATCAGAGAAATGATAGTGGTTCATCCAGATAAAGATTTTCTTGATGACATAGCCGGGAAGTTAAGAGAGGTATCTATATCCTGAAAGCTAATATCATATTTACTTGAGGCATGTCTTAGGGTTGGGAACTCCTTTTGATTTCCAATCATTATGATGTTTTGGGAGTTTCACACGATGCACTTGGAATGTCTATTGTCTCATTCTATCATAAATATTGGGGCTATTCTATTGAGCCTTTTGTCTTGGGTTTTCTTGGTACTTTAATGCTTAATATTATCTATTTCTCTTTCCGTTAGTTTAATTTCTTACTTCTTGATCTTTTCCTAATTGTGTTGCAGTACGTTCTTGAGGAATTAAATATAAGATCTCTTATCCCATGCAATGATACATTGAAGTATGCATCCCTTCGTGCTGAGCCGGATTTTAGGTATAAAAAACTTCCACTTGAAAGAATCTTCTTCTCTTTGTCCAGATGTTTTGATATGATTGTTCTCTTCTAGTATGTTGGGAAAGCGTCTTGGAAAAGCTATGGGAGTCGTGGCAAAGGAAGTCAAAGCAATGTCACAGGAAGATATCTTGGCTTTTGAGAAAGTGGGAGAAGTTACAATTGCTACTCATTGCTTAAAATTATCTGAAATAAAGGTATCTGCTTTCACTTTGAAGGCAGCAATTCTGTCTGTTCTTGATAGAATGTACTGTTTGATCCTGAATTGAATGATATGTCTGTTCATGAGGTTGAATCATAGGTGAATTTGTATGTATGTGCCTTTGAGGGTAGATATAATTTTACTATTTTTTAAGAGCTTTTTTGTTAACTCTTTACCCTTAAGGGTAAGAAAGGCCTCCTGTCCTATTCTATATGGGCCATCTATGTTTCTCAAGGAAAACTTTGGTTTCTCTTAACTGAGATTCTTGGAAGTGGTTCACACATCTCGTTACGTATCTGCTTCTGATCATATTATTGCGGAAGAAAATTTCAAGTTGTGAACCAAATTATGAAAGTTGATGTTTTAGGCCTCTAGGAATCTTGCTGATGGTGAACCTTTTCTTTCTCGTGCTATCTTGGGAGAATTGAGGTGTCAGGTGTTGTGGTGTAGTTGGTTATCATTTTGGTCTGTGATGTGCAGGGAGGGCCATATTTTTCAACCGTTGGTAGTTTTTCTTTTGAAGAACTAATTAAGGCATACAAATTTCTAACTTCCCCCAAAATCCACTGTTGCCTTCAGGTTGTCCGTGATTTCAAGCGTCCTGATGGTATGACGGACAAGGAAATTGATGCTGCTGGTGATGGTGAGAAAATTTAACAAATCAATGTTATTCAACTCTTCAAGGGTCTTAAATCTCACTCTTTGCTTGTGCTTCATTTTAGGTGATGTACTAGTGATTTTGGATTTACGACCAGATGAATCTCTATTTGAGGCTGGTGTCGCTCGTGAGGTATCTTGCATTTTCCATGCTTCATCTCTATATATGATATACTATAATCATGAATACTCTGTACCATGTTAACTTGTTTAAAGTTGGTTGTAATAGTTGCATTGTATCCGTGGTCAAAGTCCCTGTGAATTGTCATTGCTTACTTGTTTATCCATTTCAGATTGTTAATAGAATCCAGAAGTTGAGGAAGAAAGCTGCCCTTGAACCTACAGATACAGTAGAGGTGTATTTTCGATCACAGGATGAGGATACATCAGTAGCGCAGAGAGTTTTACAAGCACAGGTCTGTAGCAAGCTACCTATTTTGGAACTACCATATCATTCTCATCTCATAACTAACTGAAATTTGATCTATTGGATAGGAACTATACATAAGTGAAGCAATCGGTTCCCCCCTGCTGCCGTCTACTGTGTTACCTTCATATGCAGTAAGTTGCATCCCATAATATATGTTATGTTGCCTGTTTTATTGAATTGTAATACAAACACTAGGACTTTGTTCATTTTATTTGTTTGAAATTATCATTTCTTTCAATATATGATATGCTGTATACACTTTCTTGAAAGAAAAAACTTCAATTTTTACAACAACGTTGTTCATTTTTCAGTAGCTCGTGGATGCATTGAGTGTCCCACTGAGTTATCTTCTGTTTGAATCTTGTTATTACTTTTTTGTTCTCCATCTTAGAGTGCTTAGCTTAAACATGAATGAAAGGGATGGTGCTGTCTTGCTCATACAGTATGTTTTGCATATCCATCATGTTGATATGAGCTTCCGGTGTTATAAAATTTGTAGGTCACGCTTGCTGAAGAAAGTTTCCATAACGTAGCTGGGATTTCATTTTCTATTATCTTGGCCAGACCGGCACCTGTGTTTAATTCAGACGCTATAACGGCTTTATATGAAGGTATATGCACTATTTCTTGCACGCACACTCCCTCGTTCTTGTACTCTCTTTCTCATGCTTGTGAGCACAATTTTAGGTAATGAGAAATTTGCCCGATCACTTGAGGTTTACTTATTATCAAGAGATCTCTCCAACTTGAAGTCTGAGTTCTCCAATGGAAATGGCAAGGTTTGTATTCTTCTCCATCTTGTGTTATAAAGGAATCTTTACGTTAAATACAATTGCTGTTCAATGAAACTATACGGGAAATGACAATATAAGAAAACTGCATCTCTTCAATCAACCAAAAATTGAAACTCCTAGAATTAGGATTAAATACTATTTTGGTTCCTATACTTTTCGCTTTAGTTTATTATAATTTCTTTACCTTGAAAATATTTGTTTTGGTTTCGCTTTAGTTCATTATGATCTCTTTGCTTTGTACATATTTATTTTGGAGGTTGTACTATGAAAATATTTTTTGTCATTCGTTCTCATTTATAAGAAACTAAAAATCACCTTTAAAAAGTTACAATCAAATTGAACTTGTAAGTTTAGGTACAAGAAATGAACTAAAACCAAGAGTTCATTATTAAAAATAATATTTAAACTCCTAAAATTTAGTTAAAAGTTTGAAGTAAAAGATCTAATTTGTCCGTTAAAATAGTCTTGTGCAAATTTGCAAGTGATTTTGAAACGGTTAGAATCACTTTTGTTATGTTCAAAACACTATGAAAACGCTTCTAATTACACAAAAGCAATTCAATATTCAATTTTACCTCTGTAAATGCAATTTTCATATCATCCAAATGATTTTGAATTATTCAACAAGGTTTTGGAGTGACTTTGTCAATTTTAAAATCACTTCCAAATGTGCCCTTAATCTTCCTCGAGATCATGCATGTTATTTCTCCTAATTTATGCCTTTCTTTTCAATATACAAAATATTTATTAGTTTCTTTTACCAGGATAAAAATGATAGAAAATTTTCTTTCTATCTGTGTTCATGAAAATTTGGAATATGAAAAAAGGATAAAACAGTTGACATTGGTTCTGTGTGAAACAGATTAGGGTTGATTTCATCGACGGGCAGCCGAGCGTGGAGGTGGTGTTGGCCGAGCATGTTTTCTTAACGGCAGGCGACCAATATTTGAAGGCTAATTCTTCTTAGAATTCATTTTTGTATATCTCTGCATATTATTACCTTTTTTCTTTCAAACACTTTGTATTTTTTTCTCTTCATTTTTCATTTATATTTTTCTTTCATATGGGATAGAATTAAATCCCCCAACTTAACAGAGTAGATGCCTACCAAAGTTTAGGGCGCACAGTTTTGTATCAAAATCTCAAGGATTATATACTCATTTTTTAAAATTTGAGTGATTCTGATTTTAATGAAACCAGTTCTTATATAGTTTTCTTACAATATAGTTCTATTATTATCCCCAATTATCATTTACTTGACATAATGATTTGGTTAATGAAACCAATTCTTATATAGTTTTCTTACAATATGATGATGAACTTTCACTTTAACCTGTGGATTTAATTGATATTATACCAATCCAATTCATTTTGATTAAAGTTGCAATATTACTTCAAGTGTATTCTTAAAAGGTTAAAGAAAGTGTGATGAAAAATACATGATTGTTATTTCTTATCATGGATACCATGGCTTGTTTTGTTGTTTGTAGGGTTTAGGGTTTAGGAACTCCTAGATTCATCTAATTTCAACTTTTTTTCTTTTTTCTTTTTCCCTAGAGCAGTTCAAATTCATTCAACCAGATTTGTGGGAGAACTTCCATAGATGAAATAAACTGAAATGTCATCTTCTTTGCAAAGGAAGTTGACTAAGAAAGAAGAATCGGCAAGATCGGGGTAAACACCACTGAGCCTAGTGTAATATTCCAGTTTGATATTTATAAACAAAATCAGAGTTAGATGTGTAATGTAAATGAGTTGATGTATAATGTAACTGAGTTGTAGAATTAACCAAGTTTCATTTGACGTCTCAACGGTCATTGTACTAAAAATAGATTTGTGGTTCACTAATAGTGTTAGCTACATCCACGAACAAAGGGAGATAACAATATTACTTGGGAATGTTCTCTGAATACAAAACGATGTTTCTAG

mRNA sequence

CAAAAACATAATTTCCTAAGGTTTACAGCAATTCTCTCTCAGCTGCTCCCTCCATCTCTCCTATATTCCTATTTCTACAGCCACTGCCACCCCACTCCGTCGCCCGCCGTCGCTCACCACACCCTCCAGGTCTCCACTCTAATCTAACCGGTTCAGCCTCCCGACTAAATCGCCGCCGCCGCAATCGGGGTTAACTAAACCGGTTATTTAATTACCTTAAAACTGAGCTGAGCAGACCAGACCAGACCAACGCAGCTGATTTAGAAACGCACTTTCAGTCCTTCAATTTCACTTCTTCAAATCAGCCGCTGACGATAACTACTAGGGTGTAGTGCCGCCATGGATGAAGTTTGCGAGGGTAAGGACTTTTCGTTCCCTAAGCATGAGGAGGGGGTGCTTGAGTATTGGTCTGATATCAAGGCATTTGAGACTCAGCTCGAGCGCACACGGGACCTTCCTGAGTATATTTTTTATGATGGGCCTCCATTCGCCACTGGTCTTCCACATTATGGCCACATATTGGCGGGGACAATCAAGGACATTGTCACCCGGTATCAGGTTATGACCGGTCATCATGTGACTCGCCGCTTTGGATGGGATTGTCATGGATTGCCTGTTGAGAATGAGATTGATCAGAAGCTGGGTATTAAGAGGAGAGACGATGTGTTGAAGATGGGGATTGACAAGTACAACGAAGAATGCAGAAGTATAGTTACGCGGTATGTGGGGGAGTGGGAAAAAATCATCACGCGTACTGGACGATGGATTGATTTTAAGAACGACTATAAGACAATGGACTTGAAGTTCATGGAGAGTGTTTGGTGGGTTTTCTCTCAGCTCTACCAGAAAGGTCTTGTCTATAAAGGTTTTAAGGTCATGCCATATAGTACTGGTTGCAAGACACCACTTTCAAACTTCGAAGCTGGCCAGAGCTACAAGGATGTCCCTGATCCTGAAATAATGGTAGCTTTTCCAGTACTTGGGGATCCCCAAAATGCGGCTTTTGTGGCTTGGACAACTACTCCGTGGACCCTTCCGAGTAATCTTGCCCTTTGTGTTAATGCCAATTTTGTTTATGTGAAGGTTCGCAACAAGGTGTCCGGAAAAGTATACGTGGTTGCTAAATCTCGATTGTCTGCTTTACCTGTCGAGAAACAAAAAGTGAATGCAGCAAATGCTAATGATGGCATTAACAAGTCAAATCCTAAGAAAAAGGGATCCTCGAATTCAAAAACAGAGAACTCAGTCGAGGACTCATTCGAGATATTGGAGGAAGTATTAGGAGCTTCTCTTGTGGGTAAAAAGTACGTGCCATTGTTTGATTACTTCAAAGAGTTTTCTGATGTGGCATTTAGAGTTGTTGCGGATAGTTATGTCACTGATGATAGTGGTACGGGCATTGTCCATTGTGCTCCTGCATTTGGTGAAGATGATTATCGTGTTTGCATTGAGAACCAAGTGATCAATAAGGGTGAGAATTTGATTGTTGCAGTCGATGATGATGGTTGCTTTATTTCAAAGATTACCGATTTTTCTGGACGTTATGTCAAGGATGCAGACAAGGATATAATTGAGGCGGTGAAGGCACAAGGAAGGCTTGTTAAATCTGGAAGCTTTACCCATTCTTATCCTTTCTGCTGGAGATCTGATACTCCCCTTATCTACAGGGCTGTTCCAAGCTGGTTTGTCCGAGTTGAGAAATTAAAGGAGAATTTACTAGAAAACAATGAGAAGACTTACTGGGTGCCTGACTTTGTGAAGGAGAAAAGGTTCCATAATTGGCTAGAGAATGCAAGAGATTGGGCTGTCAGTCGTAGCAGATTTTGGGGAACACCTCTCCCTGTCTGGGTTAGCGAGGATGGGGAGGAAATACTGGTCATGGACTCAATTGAGAAACTTGAAAAGCTTTCTGGTGTTAAGGTGTTTGATCTGCATCGTCACAAGATCGATCACATTACTATTCCATCCAGAAGAGGTCCTGAATATGGTGTTCTTCGGAGGGTAGATGATGTGTTTGATTGCTGGTTTGAGAGTGGGTCTATGCCATATGCTTACATTCATTATCCATTCGAGAACGTTGAGCTTTTTGAGAAGAATTTTCCTGGGCACTTTGTGGCAGAGGGGCTTGATCAAACTCGTGGATGGTTCTACACTCTCATGGTTCTATCTACTGCTTTATTTGGGAAGCCTGCATTTAGGAACCTTATTTGTAATGGCCTTGTGCTTGCTGAGGATGGTAAGAAGATGAGTAAAAGGTTGAAAAACTATCCTTCACCAATGGAAATCATAAATGATTATGGAGCTGATGCTCTGAGATTATATCTTATAAATTCTCCAGTTGTACGTGCAGAGACTTTGCGATTCAAGAAGGAGGGTGTCTTTGGTGTTGTCAGGGATGTATTCCTGCCATGGTACAATGCATATAGATTCCTTGTTCAGAATGCCAAGAGGCTCGAGATTGAGGGGTTTGCACCATTTTCTCCAGTGGATCAGGCCACCCTTCAAAAATCATTCAATGTGCTTGACCAATGGATAAACTCTGCCACCCAGAGTTTAGTATATTTTGTCAGGAAAGAAATGGATGGCTATCGACTCTATACGGTTGTTCCATATCTTCTAAAGTTCCTTGACAACCTTACAAATATTTATGTGAGGTTCAACCGCAAGAGACTAAAAGGCCGTACTGGGGAAGAAGATTGCAGGATAGCACTCTCTACTCTGTACCATGTCCTTTTAACATCTTGTAAGGTCATGGCTCCATTTACTCCATTTTTCACTGAAGTTTTATATCAAAACTTGCGGAAAGTTTCTGATGGGTCAGAGGAAAGCATTCATTACTGTTCTTATCCTCAAGAAGGAGGAAAGAGAGGGGAAAGAATTGAAGAAAGTGTTACAAGGATGATGACAATTATTGATCTTGCTCGCAACATTCGGGAGCGTCATAACAAACCTCTTAAAGCGCCTTTGAGAGAAATGATAGTGGTTCATCCAGATAAAGATTTTCTTGATGACATAGCCGGGAAGTTAAGAGAGTACGTTCTTGAGGAATTAAATATAAGATCTCTTATCCCATGCAATGATACATTGAAGTATGCATCCCTTCGTGCTGAGCCGGATTTTAGTATGTTGGGAAAGCGTCTTGGAAAAGCTATGGGAGTCGTGGCAAAGGAAGTCAAAGCAATGTCACAGGAAGATATCTTGGCTTTTGAGAAAGTGGGAGAAGTTACAATTGCTACTCATTGCTTAAAATTATCTGAAATAAAGGTTGTCCGTGATTTCAAGCGTCCTGATGGTATGACGGACAAGGAAATTGATGCTGCTGGTGATGGTGATGTACTAGTGATTTTGGATTTACGACCAGATGAATCTCTATTTGAGGCTGGTGTCGCTCGTGAGATTGTTAATAGAATCCAGAAGTTGAGGAAGAAAGCTGCCCTTGAACCTACAGATACAGTAGAGGTGTATTTTCGATCACAGGATGAGGATACATCAGTAGCGCAGAGAGTTTTACAAGCACAGGAACTATACATAAGTGAAGCAATCGGTTCCCCCCTGCTGCCGTCTACTGTGTTACCTTCATATGCAGTCACGCTTGCTGAAGAAAGTTTCCATAACGTAGCTGGGATTTCATTTTCTATTATCTTGGCCAGACCGGCACCTGTGTTTAATTCAGACGCTATAACGGCTTTATATGAAGGTAATGAGAAATTTGCCCGATCACTTGAGGTTTACTTATTATCAAGAGATCTCTCCAACTTGAAGTCTGAGTTCTCCAATGGAAATGGCAAGATTAGGGTTGATTTCATCGACGGGCAGCCGAGCGTGGAGGTGGTGTTGGCCGAGCATGTTTTCTTAACGGCAGGCGACCAATATTTGAAGGCTAATTCTTCTTAGAATTCATTTTTGTATATCTCTGCATATTATTACCTTTTTTCTTTCAAACACTTTGTATTTTTTTCTCTTCATTTTTCATTTATATTTTTCTTTCATATGGGATAGAATTAAATCCCCCAACTTAACAGAGTAGATGCCTACCAAAGTTTAGGGCGCACAGTTTTGTATCAAAATCTCAAGGATTATATACTCATTTTTTAAAATTTGAGTGATTCTGATTTTAATGAAACCAGTTCTTATATAGTTTTCTTACAATATAGTTCTATTATTATCCCCAATTATCATTTACTTGACATAATGATTTGGTTAATGAAACCAATTCTTATATAGTTTTCTTACAATATGATGATGAACTTTCACTTTAACCTGTGGATTTAATTGATATTATACCAATCCAATTCATTTTGATTAAAGTTGCAATATTACTTCAAGTGTATTCTTAAAAGGTTAAAGAAAGTGTGATGAAAAATACATGATTGTTATTTCTTATCATGGATACCATGGCTTGTTTTGTTGTTTGTAGGGTTTAGGGTTTAGGAACTCCTAGATTCATCTAATTTCAACTTTTTTTCTTTTTTCTTTTTCCCTAGAGCAGTTCAAATTCATTCAACCAGATTTGTGGGAGAACTTCCATAGATGAAATAAACTGAAATGTCATCTTCTTTGCAAAGGAAGTTGACTAAGAAAGAAGAATCGGCAAGATCGGGGTAAACACCACTGAGCCTAGTGTAATATTCCAGTTTGATATTTATAAACAAAATCAGAGTTAGATGTGTAATGTAAATGAGTTGATGTATAATGTAACTGAGTTGTAGAATTAACCAAGTTTCATTTGACGTCTCAACGGTCATTGTACTAAAAATAGATTTGTGGTTCACTAATAGTGTTAGCTACATCCACGAACAAAGGGAGATAACAATATTACTTGGGAATGTTCTCTGAATACAAAACGATGTTTCTAG

Coding sequence (CDS)

ATGGATGAAGTTTGCGAGGGTAAGGACTTTTCGTTCCCTAAGCATGAGGAGGGGGTGCTTGAGTATTGGTCTGATATCAAGGCATTTGAGACTCAGCTCGAGCGCACACGGGACCTTCCTGAGTATATTTTTTATGATGGGCCTCCATTCGCCACTGGTCTTCCACATTATGGCCACATATTGGCGGGGACAATCAAGGACATTGTCACCCGGTATCAGGTTATGACCGGTCATCATGTGACTCGCCGCTTTGGATGGGATTGTCATGGATTGCCTGTTGAGAATGAGATTGATCAGAAGCTGGGTATTAAGAGGAGAGACGATGTGTTGAAGATGGGGATTGACAAGTACAACGAAGAATGCAGAAGTATAGTTACGCGGTATGTGGGGGAGTGGGAAAAAATCATCACGCGTACTGGACGATGGATTGATTTTAAGAACGACTATAAGACAATGGACTTGAAGTTCATGGAGAGTGTTTGGTGGGTTTTCTCTCAGCTCTACCAGAAAGGTCTTGTCTATAAAGGTTTTAAGGTCATGCCATATAGTACTGGTTGCAAGACACCACTTTCAAACTTCGAAGCTGGCCAGAGCTACAAGGATGTCCCTGATCCTGAAATAATGGTAGCTTTTCCAGTACTTGGGGATCCCCAAAATGCGGCTTTTGTGGCTTGGACAACTACTCCGTGGACCCTTCCGAGTAATCTTGCCCTTTGTGTTAATGCCAATTTTGTTTATGTGAAGGTTCGCAACAAGGTGTCCGGAAAAGTATACGTGGTTGCTAAATCTCGATTGTCTGCTTTACCTGTCGAGAAACAAAAAGTGAATGCAGCAAATGCTAATGATGGCATTAACAAGTCAAATCCTAAGAAAAAGGGATCCTCGAATTCAAAAACAGAGAACTCAGTCGAGGACTCATTCGAGATATTGGAGGAAGTATTAGGAGCTTCTCTTGTGGGTAAAAAGTACGTGCCATTGTTTGATTACTTCAAAGAGTTTTCTGATGTGGCATTTAGAGTTGTTGCGGATAGTTATGTCACTGATGATAGTGGTACGGGCATTGTCCATTGTGCTCCTGCATTTGGTGAAGATGATTATCGTGTTTGCATTGAGAACCAAGTGATCAATAAGGGTGAGAATTTGATTGTTGCAGTCGATGATGATGGTTGCTTTATTTCAAAGATTACCGATTTTTCTGGACGTTATGTCAAGGATGCAGACAAGGATATAATTGAGGCGGTGAAGGCACAAGGAAGGCTTGTTAAATCTGGAAGCTTTACCCATTCTTATCCTTTCTGCTGGAGATCTGATACTCCCCTTATCTACAGGGCTGTTCCAAGCTGGTTTGTCCGAGTTGAGAAATTAAAGGAGAATTTACTAGAAAACAATGAGAAGACTTACTGGGTGCCTGACTTTGTGAAGGAGAAAAGGTTCCATAATTGGCTAGAGAATGCAAGAGATTGGGCTGTCAGTCGTAGCAGATTTTGGGGAACACCTCTCCCTGTCTGGGTTAGCGAGGATGGGGAGGAAATACTGGTCATGGACTCAATTGAGAAACTTGAAAAGCTTTCTGGTGTTAAGGTGTTTGATCTGCATCGTCACAAGATCGATCACATTACTATTCCATCCAGAAGAGGTCCTGAATATGGTGTTCTTCGGAGGGTAGATGATGTGTTTGATTGCTGGTTTGAGAGTGGGTCTATGCCATATGCTTACATTCATTATCCATTCGAGAACGTTGAGCTTTTTGAGAAGAATTTTCCTGGGCACTTTGTGGCAGAGGGGCTTGATCAAACTCGTGGATGGTTCTACACTCTCATGGTTCTATCTACTGCTTTATTTGGGAAGCCTGCATTTAGGAACCTTATTTGTAATGGCCTTGTGCTTGCTGAGGATGGTAAGAAGATGAGTAAAAGGTTGAAAAACTATCCTTCACCAATGGAAATCATAAATGATTATGGAGCTGATGCTCTGAGATTATATCTTATAAATTCTCCAGTTGTACGTGCAGAGACTTTGCGATTCAAGAAGGAGGGTGTCTTTGGTGTTGTCAGGGATGTATTCCTGCCATGGTACAATGCATATAGATTCCTTGTTCAGAATGCCAAGAGGCTCGAGATTGAGGGGTTTGCACCATTTTCTCCAGTGGATCAGGCCACCCTTCAAAAATCATTCAATGTGCTTGACCAATGGATAAACTCTGCCACCCAGAGTTTAGTATATTTTGTCAGGAAAGAAATGGATGGCTATCGACTCTATACGGTTGTTCCATATCTTCTAAAGTTCCTTGACAACCTTACAAATATTTATGTGAGGTTCAACCGCAAGAGACTAAAAGGCCGTACTGGGGAAGAAGATTGCAGGATAGCACTCTCTACTCTGTACCATGTCCTTTTAACATCTTGTAAGGTCATGGCTCCATTTACTCCATTTTTCACTGAAGTTTTATATCAAAACTTGCGGAAAGTTTCTGATGGGTCAGAGGAAAGCATTCATTACTGTTCTTATCCTCAAGAAGGAGGAAAGAGAGGGGAAAGAATTGAAGAAAGTGTTACAAGGATGATGACAATTATTGATCTTGCTCGCAACATTCGGGAGCGTCATAACAAACCTCTTAAAGCGCCTTTGAGAGAAATGATAGTGGTTCATCCAGATAAAGATTTTCTTGATGACATAGCCGGGAAGTTAAGAGAGTACGTTCTTGAGGAATTAAATATAAGATCTCTTATCCCATGCAATGATACATTGAAGTATGCATCCCTTCGTGCTGAGCCGGATTTTAGTATGTTGGGAAAGCGTCTTGGAAAAGCTATGGGAGTCGTGGCAAAGGAAGTCAAAGCAATGTCACAGGAAGATATCTTGGCTTTTGAGAAAGTGGGAGAAGTTACAATTGCTACTCATTGCTTAAAATTATCTGAAATAAAGGTTGTCCGTGATTTCAAGCGTCCTGATGGTATGACGGACAAGGAAATTGATGCTGCTGGTGATGGTGATGTACTAGTGATTTTGGATTTACGACCAGATGAATCTCTATTTGAGGCTGGTGTCGCTCGTGAGATTGTTAATAGAATCCAGAAGTTGAGGAAGAAAGCTGCCCTTGAACCTACAGATACAGTAGAGGTGTATTTTCGATCACAGGATGAGGATACATCAGTAGCGCAGAGAGTTTTACAAGCACAGGAACTATACATAAGTGAAGCAATCGGTTCCCCCCTGCTGCCGTCTACTGTGTTACCTTCATATGCAGTCACGCTTGCTGAAGAAAGTTTCCATAACGTAGCTGGGATTTCATTTTCTATTATCTTGGCCAGACCGGCACCTGTGTTTAATTCAGACGCTATAACGGCTTTATATGAAGGTAATGAGAAATTTGCCCGATCACTTGAGGTTTACTTATTATCAAGAGATCTCTCCAACTTGAAGTCTGAGTTCTCCAATGGAAATGGCAAGATTAGGGTTGATTTCATCGACGGGCAGCCGAGCGTGGAGGTGGTGTTGGCCGAGCATGTTTTCTTAACGGCAGGCGACCAATATTTGAAGGCTAATTCTTCTTAG

Protein sequence

MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPSYAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNLKSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS*
Homology
BLAST of CSPI04G03200 vs. ExPASy Swiss-Prot
Match: F4JLM5 (Isoleucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g10320 PE=2 SV=1)

HSP 1 Score: 1893.6 bits (4904), Expect = 0.0e+00
Identity = 918/1195 (76.82%), Postives = 1034/1195 (86.53%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL++K LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDG-INKSNPKKKGSSNSKT 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K   +N   G   K+NPK KG+     
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAK---- 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
              S  DS+E+LE+  GASLVGKKY PLFDYF +FS  AFRVVAD YVTDDSGTGIVHCAP
Sbjct: 301  PESAADSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVC+ N++I KGENL+VAVDDDG F  +IT FSGRYVKDADKDIIEAVKA+GR
Sbjct: 361  AFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFH 480
            LVK+GSFTHSYPFCWRSDTPLIYRAVPSWFVRVE+LKE LL++NE+T WVP +VK+KRFH
Sbjct: 421  LVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHI 540
            NWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLEKLSGVKVFDLHRH IDHI
Sbjct: 481  NWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDHI 540

Query: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQT
Sbjct: 541  TIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGADA+R
Sbjct: 601  RGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  PF P D A
Sbjct: 661  LYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            T+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P  
Sbjct: 781  GRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPR 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
             G  GERIE SVTRMM IIDLARNIRER+  PLK PL+EMIVVHPD DFL+DI G LREY
Sbjct: 841  EGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLREY 900

Query: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELN+RSL+PCNDTLKYASL+AEPDFS+LGKRLGK+MG+VAKEVK MSQ+DILAFE+ 
Sbjct: 901  VLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEEA 960

Query: 961  GEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIA H LK ++IK+VR FKRPD + + EID+AGDGDVLVILDLR D+SL EAG ARE
Sbjct: 961  GEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKK+ LEPTD VEVYF+S DED SV+++VL +QE  I ++IGS LL S+++P
Sbjct: 1021 IVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGSTLLLSSLMP 1080

Query: 1081 SYAVTLAEESF----------HNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLE 1140
            S+AV +A+E+F            V  +S+ I LARPA  FN +A+ ALY G+ K A  L+
Sbjct: 1081 SHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGDVKSATGLQ 1140

Query: 1141 VYLLSRDLSNLKSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
             YLLSRD SNLKSEF  G+GKI V  I+  P   VVL EH+ L+ GD  L   ++
Sbjct: 1141 TYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSKRNA 1190

BLAST of CSPI04G03200 vs. ExPASy Swiss-Prot
Match: Q8BU30 (Isoleucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Iars1 PE=1 SV=2)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 592/1091 (54.26%), Postives = 771/1091 (70.67%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ SFP  EE +LE+WS    F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGIK  +DV KMGI +YN++CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCK 187
            Y  EW+  +TR GRWIDF NDYKT+  +FMESVWWVF QLY KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACG 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +N + VAWTTTPWTLPSNLALCVN    YV
Sbjct: 187  TPLSNFESNQNYKDVQDPSVFVTFP-LEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTENSVEDSF 307
            K+++   GK++++ ++RLSAL   KQ                              E  +
Sbjct: 247  KIKDVARGKLFILTEARLSAL--YKQ------------------------------ESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYF-KEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GASL GKKY PLFDYF K   + AF V+ D YV D+ GTG+VH AP FG DD+
Sbjct: 307  EILERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDH 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 427
            RVC++  +I K    +  VD  GCF  ++T F G+YVKDADK+II  +K QGRL+ +G+F
Sbjct: 367  RVCMDFNIIQKDSVPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTF 426

Query: 428  THSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNWLENAR 487
            THSYPFCWRSDTPLIY++VPSWFVRVE + + LL+NN+  YWVP+FV+EKRF NWL+ AR
Sbjct: 427  THSYPFCWRSDTPLIYKSVPSWFVRVEPMVDQLLKNNDLCYWVPEFVREKRFGNWLKEAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSRRG 547
            DWA+SR+R+WGTP+P+WVSED EE++ + S+ +LE+LSG K+ DLHR  IDH+TIPSR G
Sbjct: 487  DWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESIDHLTIPSRCG 546

Query: 548  PEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 607
                 LRRV +VFDCWFESGSMPYA +HYPFE+   FE  FP  F+AEG+DQTRGWFYTL
Sbjct: 547  K--APLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRGWFYTL 606

Query: 608  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 667
            +VL+TALFG+P F+N+I NGL+LA DG+KMSKR KNYP P+ II+ YGADALRLYLINSP
Sbjct: 607  LVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINSP 666

Query: 668  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 727
            VVRAE LRFK+EGV  V++DV LPWYNAYRF +QN  RL  E    F   ++ T+++S N
Sbjct: 667  VVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFL-YNEHTVRESPN 726

Query: 728  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 787
            + D+W+ S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG +G ED
Sbjct: 727  ITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVED 786

Query: 788  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEG 847
            C +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       + D    SIHY   P+  
Sbjct: 787  CVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVR 846

Query: 848  GKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 907
             +   ++ E +V+RM ++I+L R IR+R   P+K PL+E++V+H D + L+DI   L +Y
Sbjct: 847  EELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALEDIR-SLEKY 906

Query: 908  VLEELNIRSLIPCNDTLKYA-SLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEK 967
            ++EELN+R +    D  KY   LRAEPD  +LGKRL  A   V   +K +S E++  F+K
Sbjct: 907  IIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMMAIKRLSNEELERFQK 966

Query: 968  VGEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1027
             G + +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+S+ + G+AR
Sbjct: 967  SGSIVVEGHELHEEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQSMVDEGMAR 1026

Query: 1028 EIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVL 1087
            E++NRIQKLRKK  L PTD + VY+ ++ E   +   V+++   +I   I +PL P  V 
Sbjct: 1027 EVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYL-NNVIESHTDFIFATIKAPLKPYPVP 1058

Query: 1088 PSYAVTLAEES 1090
             S  + + E++
Sbjct: 1087 TSDNILIQEQT 1058

BLAST of CSPI04G03200 vs. ExPASy Swiss-Prot
Match: P41252 (Isoleucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=IARS1 PE=1 SV=2)

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 588/1106 (53.16%), Postives = 774/1106 (69.98%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ +FP  EE +LE+W++   F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGI+  +DV KMGI +YN +CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCK 187
            Y  EW+  ++R GRWIDF NDYKT+  +FMESVWWVF QLY KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACN 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +  + VAWTTTPWTLPSNLA+CVN    YV
Sbjct: 187  TPLSNFESHQNYKDVQDPSVFVTFP-LEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTENSVEDSF 307
            K+++   G++ ++ ++RLSAL                                  +E  +
Sbjct: 247  KIKDVARGRLLILMEARLSAL--------------------------------YKLESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYF-KEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GA L GKKY PLFDYF K   + AF V+ D+YV ++ GTG+VH AP FG +DY
Sbjct: 307  EILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDY 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 427
            RVC++  +I K    +  VD  GCF +++TDF+G+YVKDADK II  +K QGRL+ + +F
Sbjct: 367  RVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTF 426

Query: 428  THSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNWLENAR 487
            THSYPFCWRSDTPLIY+AVPSWFVRVE + + LL NN+  YWVP+ V+EKRF NWL++AR
Sbjct: 427  THSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSRRG 547
            DW +SR+R+WGTP+P+WVS+D EE++ + S+ +LE+LSG K+ DLHR  +DH+TIPSR G
Sbjct: 487  DWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCG 546

Query: 548  PEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 607
               G L R+ +VFDCWFESGSMPYA +HYPFEN   FE  FP  F+AEG+DQTRGWFYTL
Sbjct: 547  K--GSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTL 606

Query: 608  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 667
            +VL+TALFG+P F+N+I NGLVLA DG+KMSKR KNYP P+ II  YGADALRLYLINSP
Sbjct: 607  LVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSP 666

Query: 668  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 727
            VVRAE LRFK+EGV  V++DV LPWYNAYRFL+QN  RL+ E    F   ++ T+++S N
Sbjct: 667  VVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFL-YNENTVRESPN 726

Query: 728  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 787
            + D+WI S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG  G ED
Sbjct: 727  ITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMED 786

Query: 788  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEG 847
            C +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       V D    SIHY   P+  
Sbjct: 787  CVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVR 846

Query: 848  GKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 907
             +   ++ E +V++M ++I+L R IR+R   P+K PL+E++V+H D + L DI   L +Y
Sbjct: 847  EELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIK-SLEKY 906

Query: 908  VLEELNIRSLIPCNDTLKYA-SLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEK 967
            ++EELN+R +    D  KY   LRAEPD  +LGKRL  A   V   +K +S E++  F+K
Sbjct: 907  IIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQK 966

Query: 968  VGEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1027
             G + +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+S+ + G+AR
Sbjct: 967  TGTIVVEGHELHDEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQSMVDEGMAR 1026

Query: 1028 EIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVL 1087
            E++NRIQKLRKK  L PTD + VY++++ E T +   V+++   +I   I +PL P  V 
Sbjct: 1027 EVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYL-NSVIESHTEFIFTTIKAPLKPYPVS 1071

Query: 1088 PSYAVTLAEESFHNVAGISFSIILAR 1105
            PS  V + E++   + G    I L R
Sbjct: 1087 PSDKVLIQEKT--QLKGSELEITLTR 1071

BLAST of CSPI04G03200 vs. ExPASy Swiss-Prot
Match: Q54YD4 (Probable isoleucine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=ileS PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 571/1077 (53.02%), Postives = 758/1077 (70.38%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EV +  DF+    E  +L+YW DIKAFET ++ +   PEY FYDGPPFATGLPHYGHI
Sbjct: 1    MEEVPQKIDFA--GEESKILKYWDDIKAFETSVKMSEGKPEYSFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKD +TRY   TGHHV RRFGWDCHGLP+E EID+  G++ ++DVLKMGI  YN+ 
Sbjct: 61   LAGTIKDTITRYAHQTGHHVERRFGWDCHGLPIEFEIDKLHGVRTKEDVLKMGIPTYNQH 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIV +Y  EWE ++ R GRWID KN+YKTMD  FMESVWWVF +L++K LVY+GFKVM
Sbjct: 121  CRSIVMKYSHEWEIVVNRMGRWIDMKNNYKTMDTPFMESVWWVFQELFKKDLVYQGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYS GC TPLSNFEA  +YKDV DP  +V+F  L D +  + +AWTTTPWTLPSNLAL V
Sbjct: 181  PYSIGCTTPLSNFEASSNYKDVSDPACVVSFQTL-DDEKVSILAWTTTPWTLPSNLALTV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            N    Y+K+ +    +++++ K+R S L                                
Sbjct: 241  NPKMEYIKINDIKRNQIFILGKNRTSIL-------------------------------- 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDV-AFRVVADSYVTDDSGTGIVHCAP 360
               +  + +LE + G  L+GKKYVP+F YF   +++  F V+   +VTDDSGTGIVH AP
Sbjct: 301  YKSDKEYTVLETMKGTDLIGKKYVPMFPYFASDANMGGFVVIGGDFVTDDSGTGIVHTAP 360

Query: 361  AFGEDDYRVCIENQVINKGE---NLIVAVDDDGCFISKITDFSGRYVKDAD--KDIIEAV 420
            A+GEDD+ VCI N VI++ +    ++ +VD +GCF S +TDF+G  VKDA+  K I   +
Sbjct: 361  AYGEDDFNVCIANGVISRDQFKRPILNSVDANGCFTSDVTDFAGMMVKDAETTKQISIYL 420

Query: 421  KAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVK 480
            K +GR+V S +  HSYP+CWRSDTPLIY+AV SWFVRVE +++ LL NN+KTYWVPDFVK
Sbjct: 421  KNKGRMVNSANLVHSYPYCWRSDTPLIYKAVGSWFVRVESIRDKLLANNDKTYWVPDFVK 480

Query: 481  EKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRH 540
            EKRF NWL+NA DWAVSR+R+WGTP+P+W+SEDGEE++V+ SI++LE+LSGV+V DLHR 
Sbjct: 481  EKRFANWLKNATDWAVSRNRYWGTPIPLWISEDGEEVVVIGSIDELERLSGVRVTDLHRE 540

Query: 541  KIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAE 600
             IDHITIPS++G   G LRR++DVFDCWFESGSMPYA  HYPFEN + FEK FP HF+AE
Sbjct: 541  SIDHITIPSQKGK--GTLRRIEDVFDCWFESGSMPYAQQHYPFENKDKFEKIFPAHFIAE 600

Query: 601  GLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYG 660
            GLDQTRGWFYTL+VLSTALF KP F+NLI NGLVLA DGKKMSKRLKNYP PME+++  G
Sbjct: 601  GLDQTRGWFYTLLVLSTALFDKPPFQNLIVNGLVLAADGKKMSKRLKNYPDPMEVVSKVG 660

Query: 661  ADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFS 720
            AD+LRLYLINSPVVRAETL+F+++GV  +++DVFLPW+NAYRF VQN  R E      F 
Sbjct: 661  ADSLRLYLINSPVVRAETLKFQEKGVQDMIKDVFLPWFNAYRFFVQNCLRFEKATNTTFQ 720

Query: 721  PVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFN 780
            P D      S NV+D+WI ++ QSL+ FVR EM  YRLYTVVP L++F+++LTN +VR N
Sbjct: 721  P-DIKVALASENVMDKWILASCQSLIAFVRAEMAAYRLYTVVPKLVRFIEDLTNWFVRLN 780

Query: 781  RKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKV--SDGSEESIH 840
            RKRLKG  G+ DC  AL+ LY VL+T C  M PFTPFFTE +YQNL+K    +   +S+H
Sbjct: 781  RKRLKGSNGDADCLAALNILYEVLMTICIAMGPFTPFFTEYMYQNLKKALPKEKQMDSVH 840

Query: 841  YCSYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 900
            Y  +P+   +    RIEE+++RM  +I+L R  R+R  KP+K PL+E +V+  ++ +L D
Sbjct: 841  YVMFPEPIQEAFNTRIEEAISRMQVVIELGRAARDRRTKPIKFPLKEFMVITENQQYLAD 900

Query: 901  IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQE 960
            +   L+ Y+LEELNI++++  +D   +  + AE D   LG RL      ++  +  ++ E
Sbjct: 901  LE-SLKSYILEELNIQNIVLTSDEGSFVVVTAEADNKRLGARLKNDFKKISPLISKLTNE 960

Query: 961  DILAFEKVGEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1020
             +  F+K   + I  H L   ++K++R +   +G T    + +G+ ++L +LDL  D +L
Sbjct: 961  QLREFQKTQTIDILGHELTSEDLKIIRKY---NGETTNS-EPSGNEEILTVLDLTVDSAL 1020

Query: 1021 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAI 1069
            +E G+ARE++NR+Q+LRKK+ L   D V +++ +++ +   A   ++    YI E I
Sbjct: 1021 YEKGLARELINRVQRLRKKSGLTFDDPVSMFYHTKEAELKTA---IENNNDYIKETI 1031

BLAST of CSPI04G03200 vs. ExPASy Swiss-Prot
Match: Q21926 (Isoleucine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=iars-1 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 587/1170 (50.17%), Postives = 760/1170 (64.96%), Query Frame = 0

Query: 9    DFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDI 68
            + +F + E+ V + W D   F+  +E ++D P + FYDGPPFATGLPHYGH+L  TIKD+
Sbjct: 10   NINFAREEDKVAQKWQDENTFQRSVELSKDRPHFTFYDGPPFATGLPHYGHMLTSTIKDV 69

Query: 69   VTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRY 128
            V R+    GH+V RRFGWD HGLPVE EID+ LGI    DV+KMGI  YN ECR IV RY
Sbjct: 70   VGRWAHQNGHYVERRFGWDTHGLPVEYEIDKTLGISGPQDVMKMGIANYNNECRKIVMRY 129

Query: 129  VGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCKT 188
             GEWEK + R GRW+DFK+DYKT+   FMESVWW FS+L++KGLVYKG KVMP+ST C T
Sbjct: 130  SGEWEKTMGRLGRWVDFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACST 189

Query: 189  PLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYVK 248
            PLSNFEAGQ+YKDV DP + V F +L  P N   VAWTTTPWTLPSNLAL V+ + +YV 
Sbjct: 190  PLSNFEAGQNYKDVVDPAVFVGFKLLDCP-NRQLVAWTTTPWTLPSNLALVVHPDMLYVV 249

Query: 249  VRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTENSVEDSFE 308
             ++K +G  YVV + RL  L                                    D+ E
Sbjct: 250  TKDKTTGIEYVVLEERLGELK----------------------------------NDNLE 309

Query: 309  ILEEVLGASLVGKKYVPLFDYFKEFSDV--AFRVVADSYVTDDSGTGIVHCAPAFGEDDY 368
            ++E++ G+ L   +Y PLF YF    +   AFRV+ D++VT DSGTG+VH AP FGE D+
Sbjct: 310  VIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEIDF 369

Query: 369  RVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSF 428
            +VC+ N VI K + +I  VD+ G + S++ D+ G YVKDADK II+ +K  G LV+    
Sbjct: 370  QVCVANGVIAKDQKMICPVDESGKYTSEVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEV 429

Query: 429  THSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNWLENAR 488
             HSYPFCWRSDTPL+Y+AVPSWF+ VE L   LL NN++TYWVP FVKEKRF NWL +AR
Sbjct: 430  KHSYPFCWRSDTPLLYKAVPSWFINVETLIPRLLANNDETYWVPAFVKEKRFANWLRDAR 489

Query: 489  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSRRG 548
            DWAVSR+RFWGTP+ +WVSEDGEE++ + SI +LE+LSG K+ DLHR  +D +TIPSR G
Sbjct: 490  DWAVSRNRFWGTPINLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVDDVTIPSRSG 549

Query: 549  PEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTL 608
               GVL+RV +VFDCWFESGSMPYA  HYPFEN ++FE NFP  F+AEG+DQTRGWFYTL
Sbjct: 550  --RGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGIDQTRGWFYTL 609

Query: 609  MVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLINSP 668
            +VLSTALF KP F+NLICNGLVLA DG KMSK  KNYP PM I+N YGADALRLYLINSP
Sbjct: 610  LVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSP 669

Query: 669  VVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFN 728
            VVR E LRF++EGV  +++DVFLPW+NAYRF VQN +  E E    F   D      S N
Sbjct: 670  VVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHETGNVF---DMNVHVASEN 729

Query: 729  VLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEED 788
            V+D+WI S T SLV FVRKEMD YRLY VV  L KF D LTNIY+R NRKR+KG  G  +
Sbjct: 730  VMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKRVKGDNGLHE 789

Query: 789  CRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ-EGGKRGE 848
               AL+ L  VL+   ++MAPFTPFF E ++ NL+KV   +EES+H+   P+ +     E
Sbjct: 790  QHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLPKPDESLIDE 849

Query: 849  RIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEELN 908
             +E  V  M  +IDL R +R+R    +K PL+EMIV++ D  FL+D+   L  Y+L ELN
Sbjct: 850  TVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQFLEDVK-SLESYILLELN 909

Query: 909  IRSLIPCNDTLKYA-SLRAEPDFSMLGKRLGKAMGVVAKEVK-AMSQEDILAFEKVGEVT 968
            +R L    D  KY  +L+AEP+F +LG RL      VA  +K  +++ ++  F   G++T
Sbjct: 910  VRKLTVSQDKQKYGITLKAEPNFKILGARLKGEQKKVADYLKNQITESELEKFLLEGKLT 969

Query: 969  IATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNR 1028
            +  H L   E+ V        G   K      D   +V++D   DESL E G+ RE+ NR
Sbjct: 970  VLGHELSSEEVAVSYAAGSDQGHGYK---THSDAKTIVMIDTTEDESLIEEGLCREVTNR 1029

Query: 1029 IQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPL-LPSTVLPSYA 1088
            +Q+LRK+A L  TDT  V+      D+ +A +V+ A+   I  A G+P+ L S    + A
Sbjct: 1030 VQRLRKQAKLVSTDTAHVHIVVSPADSQLA-KVVAAKLNDIVSATGTPIKLGSPPAAAKA 1089

Query: 1089 VTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEK---FARSLEVYL--LSRDL 1148
             T   +S    + +   +     A   + D IT + +G++K     ++ + +L   +  L
Sbjct: 1090 PTATSKSAIKDSEVELWLF----AEGDSFDGITVV-DGSKKVRVHVKTEKEHLKGYADLL 1129

Query: 1149 SNLKSEFSNGNGKIRVDFIDG---QPSVEV 1165
             +++S     NGKI +  +DG    P+V+V
Sbjct: 1150 YHVRSALDLWNGKIALKNVDGSAVHPTVDV 1129

BLAST of CSPI04G03200 vs. ExPASy TrEMBL
Match: A0A0A0KTX5 (Isoleucyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G013540 PE=3 SV=1)

HSP 1 Score: 2407.9 bits (6239), Expect = 0.0e+00
Identity = 1184/1184 (100.00%), Postives = 1184/1184 (100.00%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1184

BLAST of CSPI04G03200 vs. ExPASy TrEMBL
Match: A0A1S3BX29 (Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494549 PE=3 SV=1)

HSP 1 Score: 2388.6 bits (6189), Expect = 0.0e+00
Identity = 1172/1184 (98.99%), Postives = 1179/1184 (99.58%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVA RVLQAQ+LYISEAIGS LLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSXLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of CSPI04G03200 vs. ExPASy TrEMBL
Match: A0A5A7TMQ4 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003320 PE=3 SV=1)

HSP 1 Score: 2375.1 bits (6154), Expect = 0.0e+00
Identity = 1167/1184 (98.56%), Postives = 1176/1184 (99.32%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW    +KLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSW----QKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDE+TSVA RVLQAQ+LYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDENTSVAHRVLQAQDLYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1180

BLAST of CSPI04G03200 vs. ExPASy TrEMBL
Match: A0A5D3CYE1 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001440 PE=3 SV=1)

HSP 1 Score: 2331.2 bits (6040), Expect = 0.0e+00
Identity = 1151/1184 (97.21%), Postives = 1158/1184 (97.80%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGF   
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGF--- 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
                               KDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  -------------------KDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVA RVLQAQ+LYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1162

BLAST of CSPI04G03200 vs. ExPASy TrEMBL
Match: A0A6J1GTY1 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457524 PE=3 SV=1)

HSP 1 Score: 2317.7 bits (6005), Expect = 0.0e+00
Identity = 1134/1185 (95.70%), Postives = 1161/1185 (97.97%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEE VLEYWSDI+AFETQLERT+DLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEVVLEYWSDIRAFETQLERTKDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQL++KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLFEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAAN-ANDGINKSNPKKKGSSNSKT 300
            NANFVYVKVRNK SGKVYVVAKSRLSALPVEKQKVNA N + + +NKSNPKKKGSSNSKT
Sbjct: 241  NANFVYVKVRNKASGKVYVVAKSRLSALPVEKQKVNAPNGSTEVVNKSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDS+E+LEEVLGASLVGKKY PLFDYFKEFSDVAFRVVAD+YVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSYEVLEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADNYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCF +KIT FSGRYVKDADKDIIEAVKAQGR
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITQFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFH 480
            LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKE LLENNEKTYWVPDFVKEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHI 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVKVFDLHRHKIDHI
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540

Query: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSC VMAPFTPFFTE LYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCMVMAPFTPFFTEALYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDDIAGKLREY
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELNIRSLIPCNDTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIATHCL+LS+IKVVRDFKRPDGMTDKE+DAAGDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GEVTIATHCLRLSDIKVVRDFKRPDGMTDKEMDAAGDGDVLVILDLRPDESLFEAGVARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKKAALEPTD+VEVYFRSQDED SVAQRVL AQELYISEAIGSPLLPS +LP
Sbjct: 1021 IVNRIQKLRKKAALEPTDSVEVYFRSQDEDASVAQRVLNAQELYISEAIGSPLLPSALLP 1080

Query: 1081 SYAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSN 1140
            S+AVTLAEESF NVAGISFSIILARP PVFNSDA+TALY+GNEKFAR+LEVYLLSRDLSN
Sbjct: 1081 SHAVTLAEESFQNVAGISFSIILARPVPVFNSDAVTALYKGNEKFARALEVYLLSRDLSN 1140

Query: 1141 LKSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            LK+EFS GNGK+RVD+IDGQP VEV L EHVFLTAGD++LKA SS
Sbjct: 1141 LKTEFS-GNGKMRVDYIDGQPGVEVALGEHVFLTAGDKFLKAKSS 1184

BLAST of CSPI04G03200 vs. NCBI nr
Match: XP_004152106.1 (isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653047.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653048.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_031739930.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN53060.1 hypothetical protein Csa_014568 [Cucumis sativus])

HSP 1 Score: 2407.9 bits (6239), Expect = 0.0e+00
Identity = 1184/1184 (100.00%), Postives = 1184/1184 (100.00%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1184

BLAST of CSPI04G03200 vs. NCBI nr
Match: XP_008453992.2 (PREDICTED: LOW QUALITY PROTEIN: isoleucine--tRNA ligase, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 2388.6 bits (6189), Expect = 0.0e+00
Identity = 1172/1184 (98.99%), Postives = 1179/1184 (99.58%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVA RVLQAQ+LYISEAIGS LLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSXLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of CSPI04G03200 vs. NCBI nr
Match: KAA0044582.1 (isoleucine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 2375.1 bits (6154), Expect = 0.0e+00
Identity = 1167/1184 (98.56%), Postives = 1176/1184 (99.32%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSW    +KLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSW----QKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDE+TSVA RVLQAQ+LYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDENTSVAHRVLQAQDLYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1180

BLAST of CSPI04G03200 vs. NCBI nr
Match: XP_038898074.1 (isoleucine--tRNA ligase, cytoplasmic isoform X2 [Benincasa hispida])

HSP 1 Score: 2350.5 bits (6090), Expect = 0.0e+00
Identity = 1154/1184 (97.47%), Postives = 1170/1184 (98.82%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NAND + KSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDSV-KSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKE LLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVE FEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVEHFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVH DKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHSDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLI C+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLITCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYF+SQDED SVAQRVL+AQELYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFKSQDEDISVAQRVLRAQELYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPA  FNSDAITALYEGNEKFAR+LEVYLLSRDL+NL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPALAFNSDAITALYEGNEKFARALEVYLLSRDLTNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEV+L EHVFLTAGDQ+LKA +S
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVLLGEHVFLTAGDQFLKAKAS 1183

BLAST of CSPI04G03200 vs. NCBI nr
Match: TYK17001.1 (isoleucine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 2331.2 bits (6040), Expect = 0.0e+00
Identity = 1151/1184 (97.21%), Postives = 1158/1184 (97.80%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGF   
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGF--- 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
                               KDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  -------------------KDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NANDGINKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF SKITDFSGRYVKDADKDIIEAVKAQGRL
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480
            VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
            IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
            YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960
            LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

Query: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLPS 1080
            VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVA RVLQAQ+LYISEAIGSPLLPSTVLPS
Sbjct: 1021 VNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSPLLPSTVLPS 1080

Query: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVYLLSRDLSNL 1140
            YAVTLAEESFHNVAGISFSIILARPAPVF+SDAITALYEGNEKFAR+LEVYLLSRDLSNL
Sbjct: 1081 YAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVYLLSRDLSNL 1140

Query: 1141 KSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
            KSEFS+GNGKIRVDFIDGQPSVEVVL EHVFLTAGDQYLKANSS
Sbjct: 1141 KSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1162

BLAST of CSPI04G03200 vs. TAIR 10
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 1893.6 bits (4904), Expect = 0.0e+00
Identity = 918/1195 (76.82%), Postives = 1034/1195 (86.53%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL++K LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDG-INKSNPKKKGSSNSKT 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K   +N   G   K+NPK KG+     
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAK---- 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
              S  DS+E+LE+  GASLVGKKY PLFDYF +FS  AFRVVAD YVTDDSGTGIVHCAP
Sbjct: 301  PESAADSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGR 420
            AFGEDDYRVC+ N++I KGENL+VAVDDDG F  +IT FSGRYVKDADKDIIEAVKA+GR
Sbjct: 361  AFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKGR 420

Query: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFH 480
            LVK+GSFTHSYPFCWRSDTPLIYRAVPSWFVRVE+LKE LL++NE+T WVP +VK+KRFH
Sbjct: 421  LVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHI 540
            NWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLEKLSGVKVFDLHRH IDHI
Sbjct: 481  NWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDHI 540

Query: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
            TIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQT
Sbjct: 541  TIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660
            RGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGADA+R
Sbjct: 601  RGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVR 660

Query: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
            LYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  PF P D A
Sbjct: 661  LYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            T+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P  
Sbjct: 781  GRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPPR 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREY 900
             G  GERIE SVTRMM IIDLARNIRER+  PLK PL+EMIVVHPD DFL+DI G LREY
Sbjct: 841  EGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLREY 900

Query: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960
            VLEELN+RSL+PCNDTLKYASL+AEPDFS+LGKRLGK+MG+VAKEVK MSQ+DILAFE+ 
Sbjct: 901  VLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEEA 960

Query: 961  GEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIA H LK ++IK+VR FKRPD + + EID+AGDGDVLVILDLR D+SL EAG ARE
Sbjct: 961  GEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFARE 1020

Query: 1021 IVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSPLLPSTVLP 1080
            IVNRIQKLRKK+ LEPTD VEVYF+S DED SV+++VL +QE  I ++IGS LL S+++P
Sbjct: 1021 IVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGSTLLLSSLMP 1080

Query: 1081 SYAVTLAEESF----------HNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLE 1140
            S+AV +A+E+F            V  +S+ I LARPA  FN +A+ ALY G+ K A  L+
Sbjct: 1081 SHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGDVKSATGLQ 1140

Query: 1141 VYLLSRDLSNLKSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1185
             YLLSRD SNLKSEF  G+GKI V  I+  P   VVL EH+ L+ GD  L   ++
Sbjct: 1141 TYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSKRNA 1190

BLAST of CSPI04G03200 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 300.4 bits (768), Expect = 6.2e-81
Identity = 232/888 (26.13%), Postives = 389/888 (43.81%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAANANDGINKSNPKKKGSSNSKTENS 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
           ++DY   ++      G  L+  VDD+G F  +   F G  V  + +  ++  +     LV
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 KSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNW 495
              S+ H YP+ WR+  P I+RA   WF  VE  +   ++      WVP      R    
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVP-HQAVNRISAM 602

Query: 496 LENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 555
             +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      
Sbjct: 603 TSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDL 662

Query: 556 IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 615
           +P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ R
Sbjct: 663 LPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHR 722

Query: 616 GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND-------- 675
           GWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I          
Sbjct: 723 GWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAP 782

Query: 676 -YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 735
            YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   
Sbjct: 783 AYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAV 842

Query: 736 PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIY 795
           P+         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y
Sbjct: 843 PY---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFY 902

Query: 796 VRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---RKVSDG 855
               + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    +  DG
Sbjct: 903 FDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDG 962

Query: 856 SEESI-----------HYCSYPQEGGKRGERIEESVTRMMTIIDLARN 864
           S                + S+P E     +R+ E  T +  +++LARN
Sbjct: 963 SAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARN 964

BLAST of CSPI04G03200 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 300.4 bits (768), Expect = 6.2e-81
Identity = 232/888 (26.13%), Postives = 389/888 (43.81%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 309  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 368

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 369  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 428

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 429  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 488

Query: 196  GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                  +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 489  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 548

Query: 256  VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAANANDGINKSNPKKKGSSNSKTENS 315
              V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 549  SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 608

Query: 316  VEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                  I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G
Sbjct: 609  ----LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHG 668

Query: 376  EDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
            ++DY   ++      G  L+  VDD+G F  +   F G  V  + +  ++  +     LV
Sbjct: 669  QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 728

Query: 436  KSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNW 495
               S+ H YP+ WR+  P I+RA   WF  VE  +   ++      WVP      R    
Sbjct: 729  MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVP-HQAVNRISAM 788

Query: 496  LENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 555
              +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      
Sbjct: 789  TSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDL 848

Query: 556  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 615
            +P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ R
Sbjct: 849  LPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHR 908

Query: 616  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND-------- 675
            GWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I          
Sbjct: 909  GWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAP 968

Query: 676  -YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 735
             YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   
Sbjct: 969  AYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAV 1028

Query: 736  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIY 795
            P+         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y
Sbjct: 1029 PY---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFY 1088

Query: 796  VRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---RKVSDG 855
                + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    +  DG
Sbjct: 1089 FDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDG 1148

Query: 856  SEESI-----------HYCSYPQEGGKRGERIEESVTRMMTIIDLARN 864
            S                + S+P E     +R+ E  T +  +++LARN
Sbjct: 1149 SAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARN 1150

BLAST of CSPI04G03200 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 293.1 bits (749), Expect = 9.9e-79
Identity = 219/831 (26.35%), Postives = 367/831 (44.16%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAANANDGINKSNPKKKGSSNSKTENS 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYV-KDADKDIIEAVKAQGRLV 435
           ++DY   ++      G  L+  VDD+G F  +   F G  V  + +  ++  +     LV
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 KSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHNW 495
              S+ H YP+ WR+  P I+RA   WF  VE  +   ++      WVP      R    
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVP-HQAVNRISAM 602

Query: 496 LENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 555
             +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   +      
Sbjct: 603 TSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDL 662

Query: 556 IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 615
           +P +   +     +  D  D WF+SGS  +A +    E +     +FP     EG DQ R
Sbjct: 663 LPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHR 722

Query: 616 GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND-------- 675
           GWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I          
Sbjct: 723 GWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAP 782

Query: 676 -YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 735
            YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     ++   
Sbjct: 783 AYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAV 842

Query: 736 PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIY 795
           P+         +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y
Sbjct: 843 PY---------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFY 902

Query: 796 VRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL 821
               + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL
Sbjct: 903 FDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNL 907

BLAST of CSPI04G03200 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 189.5 bits (480), Expect = 1.5e-47
Identity = 225/957 (23.51%), Postives = 363/957 (37.93%), Query Frame = 0

Query: 8   KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
           K F F   EE + ++W     F+   ++      ++    PP  TG  H GH +  T++D
Sbjct: 71  KTFDF-SSEEKIYKWWESQGYFKPNFDQGGS--PFVIPMPPPNVTGSLHMGHAMFVTLED 130

Query: 68  IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKL---GIKRRDDVLKMGIDKYNEECRSI 127
           I+ RY  M G       G D  G+  +  +++ L   GIKR D    +G D++ +     
Sbjct: 131 IMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVD----LGRDEFTKRVWEW 190

Query: 128 VTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVMPYST 187
             +Y G     I R G   D+  +  T+D +   +V   F +L+ KGL+Y+G  ++ +S 
Sbjct: 191 KEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSP 250

Query: 188 GCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANF 247
             +T +S+ E    Y + P     + + V G P        TT P TL  ++AL V+   
Sbjct: 251 NLQTAVSDLEV--EYSEEPGFLYHIKYRVAGSPDFLTIA--TTRPETLFGDVALAVHP-- 310

Query: 248 VYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTENSVE 307
                                                                       
Sbjct: 311 ------------------------------------------------------------ 370

Query: 308 DSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGED 367
                 E+   +  VG+  +    Y +        ++AD YV  D GTG++  +P    +
Sbjct: 371 ------EDDRYSKYVGQTAIVPMTYGRHVP-----IIADKYVDKDFGTGVLKISPGHDHN 430

Query: 368 DYRVCIE-----NQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGR 427
           DY +  +       V+NK   L    +D       +  F  R    AD + I      G 
Sbjct: 431 DYLLARKLGLPILNVMNKDATL----NDVAGLFCGLDRFEVREKLWADLEEI------GL 490

Query: 428 LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLL---ENNEKTYWVPDFVKEK 487
            VK    T   P   R    +       WFV ++ L E  L   EN E T     F  EK
Sbjct: 491 AVKKEPHTLRVPRSQRGGEVIEPLVSKQWFVHMDPLAEKALLAVENKELTIIPERF--EK 550

Query: 488 RFHNWLENARDWAVSRSRFWGTPLPVW--VSEDGEE--ILVMDSIEKLEKLSGVKVFDLH 547
            +++WL N +DW +SR  +WG  +PVW  V +D EE  I+   + E LEK        L 
Sbjct: 551 IYNHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIVAKSAEEALEKA-------LE 610

Query: 548 RHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFV 607
           ++  D   +   + P         DV D WF S   P++ + +P    + F   +P + +
Sbjct: 611 KYGKD---VEIYQDP---------DVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNML 670

Query: 608 AEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND 667
             G D    W   ++++     G   F ++  +GL+    G+KMSK L N   P++ I D
Sbjct: 671 ETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKD 730

Query: 668 YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLE----IE 727
           +G DALR  +        + L    E +          W NA +F++ +   L      E
Sbjct: 731 FGTDALRFTIALG--TAGQDLNLSTERLTANKAFTNKLW-NAGKFVLHSLPSLSDTSAWE 790

Query: 728 GFAPFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFL-DNLT 787
                  +D+     S  + + W  S    L+  V    +      V      F   +  
Sbjct: 791 NLLDLK-LDKEETLLSLPLPECWAVSKLHILIDSVTASYEKLFFGDVGRETYDFFWSDFA 850

Query: 788 NIYVRFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGS 847
           + Y+  ++ RL G  G      + + L +V     K++ PF PF TE L+Q L       
Sbjct: 851 DWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQAL----PYR 892

Query: 848 EESIHYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKD 907
           +E++    +PQ    R     ES+ R   +  L R IR           R    V P K 
Sbjct: 911 KEALIVSPWPQNSLPRN---VESIKRFENLQALTRAIRN---------ARAEYSVEPVKR 892

Query: 908 FLDDIAG--KLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVA 943
               + G  ++ EY+ +E  + +L+   D        A P  + L   L  + G+ A
Sbjct: 971 ISASVVGSAEVIEYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 892

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JLM50.0e+0076.82Isoleucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g1032... [more]
Q8BU300.0e+0054.26Isoleucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Iars1 PE=1 SV=2[more]
P412520.0e+0053.16Isoleucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=IARS1 PE=1 SV=2[more]
Q54YD40.0e+0053.02Probable isoleucine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=446... [more]
Q219260.0e+0050.17Isoleucine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=iars-1... [more]
Match NameE-valueIdentityDescription
A0A0A0KTX50.0e+00100.00Isoleucyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G013540 PE=3 SV=1[more]
A0A1S3BX290.0e+0098.99Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494549 PE=3 SV=1[more]
A0A5A7TMQ40.0e+0098.56Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5D3CYE10.0e+0097.21Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1GTY10.0e+0095.70Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457524 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004152106.10.0e+00100.00isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653047.1 isoleucin... [more]
XP_008453992.20.0e+0098.99PREDICTED: LOW QUALITY PROTEIN: isoleucine--tRNA ligase, cytoplasmic-like [Cucum... [more]
KAA0044582.10.0e+0098.56isoleucine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_038898074.10.0e+0097.47isoleucine--tRNA ligase, cytoplasmic isoform X2 [Benincasa hispida][more]
TYK17001.10.0e+0097.21isoleucine--tRNA ligase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G10320.10.0e+0076.82tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.16.2e-8126.13tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.36.2e-8126.13tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.29.9e-7926.35tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G16715.11.5e-4723.51ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002301Isoleucine-tRNA ligasePRINTSPR00984TRNASYNTHILEcoord: 42..53
score: 63.99
coord: 223..246
score: 61.16
coord: 554..567
score: 59.95
coord: 594..603
score: 83.93
IPR002301Isoleucine-tRNA ligaseTIGRFAMTIGR00392TIGR00392coord: 9..886
e-value: 3.0E-275
score: 913.6
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 302..673
e-value: 3.0E-126
score: 422.1
coord: 19..271
e-value: 5.6E-93
score: 312.2
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 4..211
e-value: 6.1E-64
score: 218.3
coord: 396..673
e-value: 6.9E-98
score: 330.2
IPR013155Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-bindingPFAMPF08264Anticodon_1coord: 729..879
e-value: 2.6E-27
score: 95.7
NoneNo IPR availableGENE3D1.10.730.10coord: 677..869
e-value: 8.5E-51
score: 174.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..300
NoneNo IPR availablePANTHERPTHR42780:SF2BNAUNNG00270D PROTEINcoord: 1..1113
NoneNo IPR availableCDDcd00818IleRS_corecoord: 40..182
e-value: 4.25465E-82
score: 269.872
NoneNo IPR availableCDDcd00818IleRS_corecoord: 397..675
e-value: 1.48102E-108
score: 341.904
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 10..685
IPR023586Isoleucine-tRNA ligase, type 2PANTHERPTHR42780SOLEUCYL-TRNA SYNTHETASEcoord: 1..1113
IPR023586Isoleucine-tRNA ligase, type 2HAMAPMF_02003Ile_tRNA_synth_type2coord: 2..1094
score: 12.715364
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 49..60
IPR033709Isoleucyl tRNA synthetase type 2, anticodon-binding domainCDDcd07961Anticodon_Ia_Ile_ABEccoord: 675..862
e-value: 2.49314E-80
score: 259.021
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 690..939
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainSUPERFAMILY50677ValRS/IleRS/LeuRS editing domaincoord: 201..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03200.1CSPI04G03200.1mRNA
CSPI04G03200.2CSPI04G03200.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
biological_process GO:0006428 isoleucyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0002161 aminoacyl-tRNA editing activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004822 isoleucine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding