CSPI04G02610 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G02610
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDNA polymerase
LocationChr4: 1577833 .. 1578459 (+)
RNA-Seq ExpressionCSPI04G02610
SyntenyCSPI04G02610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCCTCGAGTTGAGACGTAAATATCAATGAGGCCCAACTTGCTAACACAAAAGTCGAAGTTTGGTCTGAGAAGCCCCTTGGTGAGAACATTAGCAACCTGTTGGCTCGAAGGGATGTATAGAATGCATACGCTCCCACTGTCAGGTCTTTCTTTGATGAAATGTCGATCAATCTCAACATGTTGAGTTCTATCATGTTGAACTGGTTTGTTGGCAATACTAATAGCGGCTTTATTATCACAAAAAAGCTTCATTGGTGTTTCACACTCTTGATGAAGATCAAACATGACTTTCTGGAGCCAAATTTCCTCACATATTCTCAAACTCATAGCTCAGTATTCGGTGTCAGCGCTGCTCCTGGCCACAACAATTTGCTTCTTACTTCTCCAAGTTACAAGATTGCCCCATGCAAAGGTACAATAACCGAAGGTAGACTTTCTGTCAACAAAAGATTTTGCTCAATCTGAGTTAGTATATGCCTCAATGGTCTTTCTGTCTGTCTTTCTAAACATTAACCATTTACCAGGTGTCGTTTTCAAGTATCTCAAAATTTTGTTGATAGCTTCCATGTGTTCCTCATAAGGAGTCTGCATAAACTAGCTGACAACACTCACAGCAAATGAAAT

mRNA sequence

CTCCCCTCGAGTTGAGACGTAAATATCAATGAGGCCCAACTTGCTAACACAAAAGTCGAAGTTTGGTCTGAGAAGCCCCTTGGTGAGAACATTAGCAACCTGTTGGCTCGAAGGGATGTATAGAATGCATACGCTCCCACTGTCAGGTCTTTCTTTGATGAAATGTCGATCAATCTCAACATGTTGAGTTCTATCATGTTGAACTGGTTTGTTGGCAATACTAATAGCGGCTTTATTATCACAAAAAAGCTTCATTGGTGTTTCACACTCTTGATGAAGATCAAACATGACTTTCTGGAGCCAAATTTCCTCACATATTCTCAAACTCATAGCTCAGTATTCGGTGTCAGCGCTGCTCCTGGCCACAACAATTTGCTTCTTACTTCTCCAAGTTACAAGATTGCCCCATGCAAAGGTACAATAACCGAAGGTAGACTTTCTGTCAACAAAAGATTTTGCTCAATCTGAGTTAGTATATGCCTCAATGGTCTTTCTGTCTGTCTTTCTAAACATTAACCATTTACCAGGTGTCGTTTTCAAGTATCTCAAAATTTTGTTGATAGCTTCCATGTGTTCCTCATAAGGAGTCTGCATAAACTAGCTGACAACACTCACAGCAAATGAAAT

Coding sequence (CDS)

ATGTCGATCAATCTCAACATGTTGAGTTCTATCATGTTGAACTGGTTTGTTGGCAATACTAATAGCGGCTTTATTATCACAAAAAAGCTTCATTGGTGTTTCACACTCTTGATGAAGATCAAACATGACTTTCTGGAGCCAAATTTCCTCACATATTCTCAAACTCATAGCTCAGTATTCGGTGTCAGCGCTGCTCCTGGCCACAACAATTTGCTTCTTACTTCTCCAAGTTACAAGATTGCCCCATGCAAAGGTACAATAACCGAAGGTAGACTTTCTGTCAACAAAAGATTTTGCTCAATCTGA

Protein sequence

MSINLNMLSSIMLNWFVGNTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVFGVSAAPGHNNLLLTSPSYKIAPCKGTITEGRLSVNKRFCSI*
Homology
[Close]
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match: A0A5A7SR18 (Chlorophyll(Ide) b reductase NOL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2254G00120 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.2e-28
Identity = 65/82 (79.27%), Postives = 69/82 (84.15%), Query Frame = 0 

Query: 3   INLNMLSSIMLNWFVGNTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVFGV 62
           INLNM SSIMLNW V NTNS FIITK+L W FT LMKI  DFLEPNFLTYSQTHSS+FG+
Sbjct: 319 INLNMFSSIMLNWVVSNTNSSFIITKELQWSFTFLMKIGQDFLEPNFLTYSQTHSSIFGL 378

Query: 63  SAAPGHNNLLLTSPSYKIAPCK 85
           + APGHN LLLT PSYKIAP K
Sbjct: 379 NTAPGHNTLLLTPPSYKIAPTK 400

[Close]
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match: A0A5D3DNS2 (Kelch-like protein 2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00450 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.0e-27
Identity = 68/90 (75.56%), Postives = 72/90 (80.00%), Query Frame = 0 

Query: 1   MSINLNMLSSIMLNWFVGNTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVF 60
           MSINLNM S IMLNW V NTNSGFIIT++L W  T LMKI+HDFLEPNFLTYSQTHS VF
Sbjct: 366 MSINLNMFSFIMLNWVVSNTNSGFIITQELQWSLTFLMKIRHDFLEPNFLTYSQTHSPVF 425

Query: 61  GVSAAPGHNNLLLTSPSYKIAPCK-GTITE 90
           G+S A G N LLLT PSYKIAP K G I E
Sbjct: 426 GLSTALGLNALLLTPPSYKIAPNKEGEILE 455

[Close]
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match: A0A5A7UPW9 (Altered inheritance of mitochondria protein 32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00180 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-18
Identity = 47/69 (68.12%), Postives = 54/69 (78.26%), Query Frame = 0 

Query: 19  NTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVFGVSAAPGHNNLLLTSPSY 78
           NTNS FII K+L W  T LMKI++DFLEP FLTYSQTH S+F ++A PGHN LL T  SY
Sbjct: 283 NTNSSFIIIKELQWSLTFLMKIRYDFLEPTFLTYSQTHCSIFNLNATPGHNTLLFTPLSY 342

Query: 79  KIAPCKGTI 88
           KIAP KGT+
Sbjct: 343 KIAPNKGTM 351

[Close]
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match: A0A5D3BHT1 (Protein CHROMATIN REMODELING 20 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold429G00030 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.6e-17
Identity = 57/104 (54.81%), Postives = 61/104 (58.65%), Query Frame = 0 

Query: 7   MLSSIMLNWFVGNTNSGFIITKKLHWC---------FTLLMKIKHDFLEPNFLTYSQTHS 66
           M SSIMLNW V +TN+ FII KKL WC          T L   K   + P       THS
Sbjct: 1   MFSSIMLNWVVNDTNNDFIIRKKLRWCRWCLTFDEDLTRLFGAKFPHIFP-------THS 60

Query: 67  SVFGVSAAPGHNNLLLTSPSYKIAPCKGTITEGRLSVNKRFCSI 102
           S+FG  AAPGHN LLLT  SYKI   KGTIT GRLSVN R C I
Sbjct: 61  SIFGFCAAPGHNTLLLTPLSYKIPLNKGTITGGRLSVNNRSCPI 97

[Close]
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match: A0A5D3DLH4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00060 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.9e-10
Identity = 36/48 (75.00%), Postives = 38/48 (79.17%), Query Frame = 0 

Query: 46  EPNFLTYSQTHSSVFGVSAAPGHNNLLLTSPSYKIAPCKGTITEGRLS 94
           +PNFLTYSQTHSSVF  SAAPGHN LLLT PSYKI   K TIT G L+
Sbjct: 179 KPNFLTYSQTHSSVFNFSAAPGHNTLLLTPPSYKIVSNKSTITGGSLN 226

[Close]
BLAST of CSPI04G02610 vs. NCBI nr
Match: KAA0031719.1 (chlorophyll(ide) b reductase NOL [Cucumis melo var. makuwa] >TYK30399.1 chlorophyll(ide) b reductase NOL [Cucumis melo var. makuwa])

HSP 1 Score: 135.6 bits (340), Expect = 2.4e-28
Identity = 65/82 (79.27%), Postives = 69/82 (84.15%), Query Frame = 0 

Query: 3   INLNMLSSIMLNWFVGNTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVFGV 62
           INLNM SSIMLNW V NTNS FIITK+L W FT LMKI  DFLEPNFLTYSQTHSS+FG+
Sbjct: 319 INLNMFSSIMLNWVVSNTNSSFIITKELQWSFTFLMKIGQDFLEPNFLTYSQTHSSIFGL 378

Query: 63  SAAPGHNNLLLTSPSYKIAPCK 85
           + APGHN LLLT PSYKIAP K
Sbjct: 379 NTAPGHNTLLLTPPSYKIAPTK 400

[Close]
BLAST of CSPI04G02610 vs. NCBI nr
Match: TYK24930.1 (kelch-like protein 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 130.2 bits (326), Expect = 1.0e-26
Identity = 68/90 (75.56%), Postives = 72/90 (80.00%), Query Frame = 0 

Query: 1   MSINLNMLSSIMLNWFVGNTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVF 60
           MSINLNM S IMLNW V NTNSGFIIT++L W  T LMKI+HDFLEPNFLTYSQTHS VF
Sbjct: 366 MSINLNMFSFIMLNWVVSNTNSGFIITQELQWSLTFLMKIRHDFLEPNFLTYSQTHSPVF 425

Query: 61  GVSAAPGHNNLLLTSPSYKIAPCK-GTITE 90
           G+S A G N LLLT PSYKIAP K G I E
Sbjct: 426 GLSTALGLNALLLTPPSYKIAPNKEGEILE 455

[Close]
BLAST of CSPI04G02610 vs. NCBI nr
Match: KAA0057244.1 (altered inheritance of mitochondria protein 32-like [Cucumis melo var. makuwa] >TYK18717.1 altered inheritance of mitochondria protein 32-like [Cucumis melo var. makuwa])

HSP 1 Score: 101.7 bits (252), Expect = 3.9e-18
Identity = 47/69 (68.12%), Postives = 54/69 (78.26%), Query Frame = 0 

Query: 19  NTNSGFIITKKLHWCFTLLMKIKHDFLEPNFLTYSQTHSSVFGVSAAPGHNNLLLTSPSY 78
           NTNS FII K+L W  T LMKI++DFLEP FLTYSQTH S+F ++A PGHN LL T  SY
Sbjct: 283 NTNSSFIIIKELQWSLTFLMKIRYDFLEPTFLTYSQTHCSIFNLNATPGHNTLLFTPLSY 342

Query: 79  KIAPCKGTI 88
           KIAP KGT+
Sbjct: 343 KIAPNKGTM 351

[Close]
BLAST of CSPI04G02610 vs. NCBI nr
Match: TYJ98677.1 (protein CHROMATIN REMODELING 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 97.4 bits (241), Expect = 7.4e-17
Identity = 57/104 (54.81%), Postives = 61/104 (58.65%), Query Frame = 0 

Query: 7   MLSSIMLNWFVGNTNSGFIITKKLHWC---------FTLLMKIKHDFLEPNFLTYSQTHS 66
           M SSIMLNW V +TN+ FII KKL WC          T L   K   + P       THS
Sbjct: 1   MFSSIMLNWVVNDTNNDFIIRKKLRWCRWCLTFDEDLTRLFGAKFPHIFP-------THS 60

Query: 67  SVFGVSAAPGHNNLLLTSPSYKIAPCKGTITEGRLSVNKRFCSI 102
           S+FG  AAPGHN LLLT  SYKI   KGTIT GRLSVN R C I
Sbjct: 61  SIFGFCAAPGHNTLLLTPLSYKIPLNKGTITGGRLSVNNRSCPI 97

[Close]
BLAST of CSPI04G02610 vs. NCBI nr
Match: TYK24465.1 (uncharacterized protein E5676_scaffold266G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 75.1 bits (183), Expect = 3.9e-10
Identity = 36/48 (75.00%), Postives = 38/48 (79.17%), Query Frame = 0 

Query: 46  EPNFLTYSQTHSSVFGVSAAPGHNNLLLTSPSYKIAPCKGTITEGRLS 94
           +PNFLTYSQTHSSVF  SAAPGHN LLLT PSYKI   K TIT G L+
Sbjct: 179 KPNFLTYSQTHSSVFNFSAAPGHNTLLLTPPSYKIVSNKSTITGGSLN 226

The following BLAST results are available for this feature:
BLAST of CSPI04G02610 vs.
Match NameE-valueIdentityDescription
BLAST of CSPI04G02610 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SR181.2e-2879.27Chlorophyll(Ide) b reductase NOL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5D3DNS25.0e-2775.56Kelch-like protein 2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5A7UPW91.9e-1868.12Altered inheritance of mitochondria protein 32-like OS=Cucumis melo var. makuwa ... [more]
A0A5D3BHT13.6e-1754.81Protein CHROMATIN REMODELING 20 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5D3DLH41.9e-1075.00Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
BLAST of CSPI04G02610 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0031719.12.4e-2879.27chlorophyll(ide) b reductase NOL [Cucumis melo var. makuwa] >TYK30399.1 chloroph... [more]
TYK24930.11.0e-2675.56kelch-like protein 2 isoform X2 [Cucumis melo var. makuwa][more]
KAA0057244.13.9e-1868.12altered inheritance of mitochondria protein 32-like [Cucumis melo var. makuwa] >... [more]
TYJ98677.17.4e-1754.81protein CHROMATIN REMODELING 20 isoform X1 [Cucumis melo var. makuwa][more]
TYK24465.13.9e-1075.00uncharacterized protein E5676_scaffold266G00060 [Cucumis melo var. makuwa][more]
BLAST of CSPI04G02610 vs. NCBI nr
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G02610.1CSPI04G02610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006260 DNA replication
biological_process GO:0034976 response to endoplasmic reticulum stress
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003677 DNA binding
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003964 RNA-directed DNA polymerase activity