CSPI04G02390 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G02390
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhenylalanine ammonia-lyase
LocationChr4: 1397267 .. 1397979 (-)
RNA-Seq ExpressionCSPI04G02390
SyntenyCSPI04G02390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTACTGCTCTGAGCTTCAATATCTTGCAAATCCTGTCACAAGTCACGTCCAAAGCGCCGAGCAACACAACCAAGATGTTAACTCTCTTGGTTTAATTTCCTCAAGGAAAACAGCTGAAGCCATCGATATTCTTAAACTCATGTCTTCCACATTCTTAGTTGCTTTATGTCAAGCAATTGACTTGAGGCATTTGGAGGAGAACTTGAAGAGCACGGTGAAAAGCACAGTTAGCCAAGTAGCAAAAAAAGTGTTGACTACCTCTTCCAATGGCGCACTTCATCCATCAAGATTTTGTGAGAAAGATTTACTCAAAGTTGTTGACAGGGAATACACATTTGCATACATTGATGATCCATGCAGTGCCACCTACCCACTTATGCAAAAGCTAAGGCAAATTATTGTTGAGCATGCACTTAAAAACGGCGATAACGAAACAAACGAAAACACCTCAATCTTCCAAAAGATTGGTGCTTTCGAAGAGGAGTTGAAGGCAATCTTACCAAAGGAGGTTGAAAACGTAAGGTTAGCATATGAGAATGGAAACTCTAAAATTGAAAATCAAATCAAGGATTGTAGATCTTACCCATTATACAAATTTGTGAGAGAGGAGTTGGGAACAAGATTGCTAACTGGCGAAAGGGTAATTTCTCCCGGAGAAGAGTGCGAGAAGGTATTCACCGCTTTAAGTCAAGGAAAGATGATTGA

mRNA sequence

ATGGCTTCTTACTGCTCTGAGCTTCAATATCTTGCAAATCCTGTCACAAGTCACGTCCAAAGCGCCGAGCAACACAACCAAGATGTTAACTCTCTTGGTTTAATTTCCTCAAGGAAAACAGCTGAAGCCATCGATATTCTTAAACTCATGTCTTCCACATTCTTAGTTGCTTTATGTCAAGCAATTGACTTGAGGCATTTGGAGGAGAACTTGAAGAGCACGGTGAAAAGCACAGTTAGCCAAGTAGCAAAAAAAGTGTTGACTACCTCTTCCAATGGCGCACTTCATCCATCAAGATTTTGTGAGAAAGATTTACTCAAAGTTGTTGACAGGGAATACACATTTGCATACATTGATGATCCATGCAGTGCCACCTACCCACTTATGCAAAAGCTAAGGCAAATTATTGTTGAGCATGCACTTAAAAACGGCGATAACGAAACAAACGAAAACACCTCAATCTTCCAAAAGATTGGTGCTTTCGAAGAGGAGTTGAAGGCAATCTTACCAAAGGAGGTTGAAAACGTAAGAGGAGTTGGGAACAAGATTGCTAACTGGCGAAAGGGTAATTTCTCCCGGAGAAGAGTGCGAGAAGGTATTCACCGCTTTAAGTCAAGGAAAGATGATTGA

Coding sequence (CDS)

ATGGCTTCTTACTGCTCTGAGCTTCAATATCTTGCAAATCCTGTCACAAGTCACGTCCAAAGCGCCGAGCAACACAACCAAGATGTTAACTCTCTTGGTTTAATTTCCTCAAGGAAAACAGCTGAAGCCATCGATATTCTTAAACTCATGTCTTCCACATTCTTAGTTGCTTTATGTCAAGCAATTGACTTGAGGCATTTGGAGGAGAACTTGAAGAGCACGGTGAAAAGCACAGTTAGCCAAGTAGCAAAAAAAGTGTTGACTACCTCTTCCAATGGCGCACTTCATCCATCAAGATTTTGTGAGAAAGATTTACTCAAAGTTGTTGACAGGGAATACACATTTGCATACATTGATGATCCATGCAGTGCCACCTACCCACTTATGCAAAAGCTAAGGCAAATTATTGTTGAGCATGCACTTAAAAACGGCGATAACGAAACAAACGAAAACACCTCAATCTTCCAAAAGATTGGTGCTTTCGAAGAGGAGTTGAAGGCAATCTTACCAAAGGAGGTTGAAAACGTAAGAGGAGTTGGGAACAAGATTGCTAACTGGCGAAAGGGTAATTTCTCCCGGAGAAGAGTGCGAGAAGGTATTCACCGCTTTAAGTCAAGGAAAGATGATTGA

Protein sequence

MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVRGVGNKIANWRKGNFSRRRVREGIHRFKSRKDD*
Homology
BLAST of CSPI04G02390 vs. ExPASy Swiss-Prot
Match: O64963 (Phenylalanine ammonia-lyase 1 OS=Prunus avium OX=42229 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.2e-82
Identity = 157/195 (80.51%), Postives = 172/195 (88.21%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+LANPVT+HVQSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQ
Sbjct: 463 MASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQ 522

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENL++TVK+TVSQVAK+ LTT  NG LHPSRFCEKDLLKVVDREY FAYIDD
Sbjct: 523 AIDLRHLEENLRNTVKNTVSQVAKRTLTTGVNGELHPSRFCEKDLLKVVDREYVFAYIDD 582

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR--- 180
           PCSATYPLMQKLRQ++VEHAL NG+NE N +TSIFQKI AFEEELK +LPKEV++ R   
Sbjct: 583 PCSATYPLMQKLRQVLVEHALTNGENEKNASTSIFQKIVAFEEELKVLLPKEVDSARAAL 642

Query: 181 -----GVGNKIANWR 188
                GV N+I   R
Sbjct: 643 DSGSAGVPNRITECR 657

BLAST of CSPI04G02390 vs. ExPASy Swiss-Prot
Match: P45730 (Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.2e-82
Identity = 151/177 (85.31%), Postives = 168/177 (94.92%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLISSRKTAEA+DILKLMS+TFLVALCQ
Sbjct: 461 MASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTFLVALCQ 520

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VK+TVSQV+K+VLTT +NG LHPSRFCEK+LLKVVDREY FAY+DD
Sbjct: 521 AIDLRHLEENLKSAVKNTVSQVSKRVLTTGANGELHPSRFCEKELLKVVDREYVFAYVDD 580

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQ+ V+HAL+NG+NE N +TS+FQKI AFEEELKA+LPKEVE+ R
Sbjct: 581 PCSATYPLMQKLRQVFVDHALENGENEKNFSTSVFQKIEAFEEELKALLPKEVESAR 637

BLAST of CSPI04G02390 vs. ExPASy Swiss-Prot
Match: P45735 (Phenylalanine ammonia-lyase (Fragment) OS=Vitis vinifera OX=29760 GN=PAL PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 1.8e-81
Identity = 150/177 (84.75%), Postives = 166/177 (93.79%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+LANPVT+HV+SAEQHNQDVNSLGLISSRKTAEA+DILKLMS+T+LVALCQ
Sbjct: 162 MASYCSELQFLANPVTNHVESAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQ 221

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKSTVK TVS VAKK LT  +NG LHPSRFCEKDLLKVVDRE+ FAYIDD
Sbjct: 222 AIDLRHLEENLKSTVKKTVSHVAKKTLTIGANGELHPSRFCEKDLLKVVDREHVFAYIDD 281

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQK+RQ++VEHAL NG++E N +TSIFQKIGAFEEELKA+LPKEVE+ R
Sbjct: 282 PCSATYPLMQKVRQVLVEHALNNGESEKNGSTSIFQKIGAFEEELKAVLPKEVESAR 338

BLAST of CSPI04G02390 vs. ExPASy Swiss-Prot
Match: Q9SMK9 (Phenylalanine ammonia-lyase 2 OS=Cicer arietinum OX=3827 GN=PAL2 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 2.4e-81
Identity = 150/177 (84.75%), Postives = 165/177 (93.22%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLISSRKTAEA++ILKLMSSTFLVALCQ
Sbjct: 464 MASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVEILKLMSSTFLVALCQ 523

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRH+EENLKS VK+TVSQVAK+VLT   NG LHPSRFCEKDLL VV+REY FAYIDD
Sbjct: 524 AIDLRHIEENLKSVVKNTVSQVAKRVLTVGVNGELHPSRFCEKDLLNVVEREYVFAYIDD 583

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLR ++V+HAL+NGD E N +TSIFQKIGAFE+ELK +LPKEVE+VR
Sbjct: 584 PCSATYPLMQKLRHVLVDHALENGDREGNSSTSIFQKIGAFEQELKTLLPKEVESVR 640

BLAST of CSPI04G02390 vs. ExPASy Swiss-Prot
Match: O23865 (Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 9.1e-81
Identity = 150/179 (83.80%), Postives = 165/179 (92.18%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+L NPVT+HVQSAEQHNQDVNSLGLISSRKTAEA++ILKLMS+TFLV LCQ
Sbjct: 454 MASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVEILKLMSTTFLVGLCQ 513

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A+DLRHLEENLKSTVK+TVSQVAKKVLT   NG LHPSRFCE DLL+VVDREY FAYIDD
Sbjct: 514 AVDLRHLEENLKSTVKNTVSQVAKKVLTMGVNGELHPSRFCELDLLRVVDREYIFAYIDD 573

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVRGV 180
           PCSATYPLMQKLRQ++VEHALKNG+ E N +TSIFQKI AFE+ELKA+LPKEVE+ R V
Sbjct: 574 PCSATYPLMQKLRQVLVEHALKNGETEKNLSTSIFQKIAAFEDELKALLPKEVESARAV 632

BLAST of CSPI04G02390 vs. ExPASy TrEMBL
Match: G8HZH6 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=PAL PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.5e-90
Identity = 175/177 (98.87%), Postives = 175/177 (98.87%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSVVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGA EEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAXEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. ExPASy TrEMBL
Match: A0A0A0KTN3 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_4G008780 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.5e-90
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSVVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGD+ETNENTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQILVEHALKNGDSETNENTSIFQKIGAFEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. ExPASy TrEMBL
Match: A0A0A0KYQ0 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_4G008250 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 8.5e-90
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 61  AIDLRHLEENLKSVVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGD+ETNENTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 121 PCSATYPLMQKLRQILVEHALKNGDSETNENTSIFQKIGAFEEELKAILPKEVENVR 177

BLAST of CSPI04G02390 vs. ExPASy TrEMBL
Match: A0A5D3E147 (Phenylalanine ammonia-lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002480 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.9e-89
Identity = 173/177 (97.74%), Postives = 175/177 (98.87%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQ
Sbjct: 142 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQ 201

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKSTVK+TVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 202 AIDLRHLEENLKSTVKNTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 261

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGDNETN NTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 262 PCSATYPLMQKLRQILVEHALKNGDNETNANTSIFQKIGAFEEELKAILPKEVENVR 318

BLAST of CSPI04G02390 vs. ExPASy TrEMBL
Match: A0A1S3BZ45 (Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103494629 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.9e-89
Identity = 173/177 (97.74%), Postives = 175/177 (98.87%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKSTVK+TVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSTVKNTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGDNETN NTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQILVEHALKNGDNETNANTSIFQKIGAFEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. NCBI nr
Match: XP_004152155.3 (phenylalanine ammonia-lyase-like [Cucumis sativus] >KAE8649079.1 hypothetical protein Csa_015294 [Cucumis sativus])

HSP 1 Score: 342.4 bits (877), Expect = 2.7e-90
Identity = 176/177 (99.44%), Postives = 176/177 (99.44%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSAVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. NCBI nr
Match: NP_001295839.1 (phenylalanine ammonia-lyase-like [Cucumis sativus] >AER58180.1 phenylalanine ammonia lyase [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 1.8e-89
Identity = 175/177 (98.87%), Postives = 175/177 (98.87%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSVVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGA EEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAXEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. NCBI nr
Match: XP_031739733.1 (phenylalanine ammonia-lyase [Cucumis sativus] >KAE8649078.1 hypothetical protein Csa_014775 [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 1.8e-89
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 459 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 518

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 519 AIDLRHLEENLKSVVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 578

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGD+ETNENTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 579 PCSATYPLMQKLRQILVEHALKNGDSETNENTSIFQKIGAFEEELKAILPKEVENVR 635

BLAST of CSPI04G02390 vs. NCBI nr
Match: KAE8649076.1 (hypothetical protein Csa_014727 [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 2.3e-89
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ
Sbjct: 54  MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 113

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKS VKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 114 AIDLRHLEENLKSEVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 173

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGD+ETNENTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 174 PCSATYPLMQKLRQILVEHALKNGDSETNENTSIFQKIGAFEEELKAILPKEVENVR 230

BLAST of CSPI04G02390 vs. NCBI nr
Match: TYK29636.1 (phenylalanine ammonia-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 338.6 bits (867), Expect = 3.9e-89
Identity = 173/177 (97.74%), Postives = 175/177 (98.87%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQ
Sbjct: 142 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQ 201

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           AIDLRHLEENLKSTVK+TVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD
Sbjct: 202 AIDLRHLEENLKSTVKNTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 261

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPLMQKLRQI+VEHALKNGDNETN NTSIFQKIGAFEEELKAILPKEVENVR
Sbjct: 262 PCSATYPLMQKLRQILVEHALKNGDNETNANTSIFQKIGAFEEELKAILPKEVENVR 318

BLAST of CSPI04G02390 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 301.2 bits (770), Expect = 6.4e-82
Identity = 152/185 (82.16%), Postives = 165/185 (89.19%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT+EA+DILKLMS+TFLV +CQ
Sbjct: 463 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKLMSTTFLVGICQ 522

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LHPSRFCEKDLLKVVDRE  F Y+DD
Sbjct: 523 AVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEKDLLKVVDREQVFTYVDD 582

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVRGV- 180
           PCSATYPLMQ+LRQ+IV+HAL NG+ E N  TSIFQKIGAFEEELKA+LPKEVE  R   
Sbjct: 583 PCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQKIGAFEEELKAVLPKEVEAARAAY 642

Query: 181 GNKIA 185
           GN  A
Sbjct: 643 GNGTA 647

BLAST of CSPI04G02390 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 296.6 bits (758), Expect = 1.6e-80
Identity = 148/177 (83.62%), Postives = 162/177 (91.53%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT+EA+DILKLMS+TFLVA+CQ
Sbjct: 471 MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKLMSTTFLVAICQ 530

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LHPSRFCEKDLLKVVDRE  + Y DD
Sbjct: 531 AVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHPSRFCEKDLLKVVDREQVYTYADD 590

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCSATYPL+QKLRQ+IV+HAL NG++E N  TSIF KIGAFEEELKA+LPKEVE  R
Sbjct: 591 PCSATYPLIQKLRQVIVDHALINGESEKNAVTSIFHKIGAFEEELKAVLPKEVEAAR 647

BLAST of CSPI04G02390 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 279.3 bits (713), Expect = 2.6e-75
Identity = 138/177 (77.97%), Postives = 156/177 (88.14%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+LANPVT+HVQSAEQHNQDVNSLGLISSRKTAEA+DILKLMS+T+LVALCQ
Sbjct: 453 MASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQ 512

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A+DLRHLEENLK  VKS VSQVAK+VLT  +NG LHPSRF E+D+L+VVDREY F+Y DD
Sbjct: 513 AVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTERDVLQVVDREYVFSYADD 572

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           PCS TYPLMQKLR I+V+HAL + + E N  TS+F KIGAFE ELK +LPKEVE VR
Sbjct: 573 PCSLTYPLMQKLRHILVDHALADPEREANSATSVFHKIGAFEAELKLLLPKEVERVR 629

BLAST of CSPI04G02390 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 207.6 bits (527), Expect = 9.7e-54
Identity = 114/177 (64.41%), Postives = 134/177 (75.71%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLISSR TAEA+ ILKLMS+T+LVALCQ
Sbjct: 450 MASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRTTAEAVVILKLMSTTYLVALCQ 509

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A DLRHLEE LK  V   VS  AK VL      A+ P R    D+L VV+REY F+Y+DD
Sbjct: 510 AFDLRHLEEILKKAVNEVVSHTAKSVL------AIEPFR-KHDDILGVVNREYVFSYVDD 569

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           P S T PLMQKLR ++ + AL   + ET+   ++F+KIGAFE ELK +LPKEVE VR
Sbjct: 570 PSSLTNPLMQKLRHVLFDKALAEPEGETD---TVFRKIGAFEAELKFLLPKEVERVR 616

BLAST of CSPI04G02390 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 207.6 bits (527), Expect = 9.7e-54
Identity = 114/177 (64.41%), Postives = 134/177 (75.71%), Query Frame = 0

Query: 1   MASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQ 60
           MASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLISSR TAEA+ ILKLMS+T+LVALCQ
Sbjct: 454 MASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRTTAEAVVILKLMSTTYLVALCQ 513

Query: 61  AIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDD 120
           A DLRHLEE LK  V   VS  AK VL      A+ P R    D+L VV+REY F+Y+DD
Sbjct: 514 AFDLRHLEEILKKAVNEVVSHTAKSVL------AIEPFR-KHDDILGVVNREYVFSYVDD 573

Query: 121 PCSATYPLMQKLRQIIVEHALKNGDNETNENTSIFQKIGAFEEELKAILPKEVENVR 178
           P S T PLMQKLR ++ + AL   + ET+   ++F+KIGAFE ELK +LPKEVE VR
Sbjct: 574 PSSLTNPLMQKLRHVLFDKALAEPEGETD---TVFRKIGAFEAELKFLLPKEVERVR 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O649632.2e-8280.51Phenylalanine ammonia-lyase 1 OS=Prunus avium OX=42229 GN=PAL1 PE=2 SV=1[more]
P457302.2e-8285.31Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1[more]
P457351.8e-8184.75Phenylalanine ammonia-lyase (Fragment) OS=Vitis vinifera OX=29760 GN=PAL PE=2 SV... [more]
Q9SMK92.4e-8184.75Phenylalanine ammonia-lyase 2 OS=Cicer arietinum OX=3827 GN=PAL2 PE=2 SV=1[more]
O238659.1e-8183.80Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
G8HZH68.5e-9098.87Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=PAL PE=2 SV=1[more]
A0A0A0KTN38.5e-9098.31Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_4G008780 PE=3 SV=1[more]
A0A0A0KYQ08.5e-9098.31Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_4G008250 PE=3 SV=1[more]
A0A5D3E1471.9e-8997.74Phenylalanine ammonia-lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BZ451.9e-8997.74Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103494629 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152155.32.7e-9099.44phenylalanine ammonia-lyase-like [Cucumis sativus] >KAE8649079.1 hypothetical pr... [more]
NP_001295839.11.8e-8998.87phenylalanine ammonia-lyase-like [Cucumis sativus] >AER58180.1 phenylalanine amm... [more]
XP_031739733.11.8e-8998.31phenylalanine ammonia-lyase [Cucumis sativus] >KAE8649078.1 hypothetical protein... [more]
KAE8649076.12.3e-8998.31hypothetical protein Csa_014727 [Cucumis sativus][more]
TYK29636.13.9e-8997.74phenylalanine ammonia-lyase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G53260.16.4e-8282.16phenylalanine ammonia-lyase 2 [more]
AT2G37040.11.6e-8083.62PHE ammonia lyase 1 [more]
AT3G10340.12.6e-7577.97phenylalanine ammonia-lyase 4 [more]
AT5G04230.19.7e-5464.41phenyl alanine ammonia-lyase 3 [more]
AT5G04230.29.7e-5464.41phenyl alanine ammonia-lyase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 207..209
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 1..67
e-value: 8.9E-83
score: 280.6
NoneNo IPR availablePANTHERPTHR10362:SF57PHENYLALANINE AMMONIA-LYASE 1-LIKEcoord: 1..177
IPR023144Phenylalanine ammonia-lyase, shielding domain superfamilyGENE3D1.10.274.20coord: 68..178
e-value: 8.9E-83
score: 280.6
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 1..75
e-value: 4.0E-19
score: 68.6
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 1..177
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 1..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G02390.1CSPI04G02390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0003824 catalytic activity