CSPI04G02100 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G02100
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionaquaporin NIP6-1
LocationChr4: 1202368 .. 1205212 (-)
RNA-Seq ExpressionCSPI04G02100
SyntenyCSPI04G02100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAGCCAAAAACAAAACAATCTTTATTAATCTTGTTTTTTTCATAGCCTTCTCTCTCTTTTGGAACTTTTGTGCTATGGATACAGAGGAAGCTCCATCAACACCAGTGACGCCGGGAACTCCTGGAGCTCCGCTGTTTGGACGAGTGAAAGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGTTGCATCTCTTTCAATGTTGATGATAATTGGGGGAGTGAAGAAGGTGGCTTAACTAAAATAGTCTCTTCTTGTTCATTGCCATACTCTCCTGTCTCACTTGCAAGAAAGGTAACCCTATATATAAAAAGAGAGCGTTTTAGTTTCTAAAGTTTTAAGCTTTACACTAACCCACATCTCATATGAAATACTTTTGTGCATTCATCTCGGTCGAGCTCATACTCAATTTGTCCAACCACGGTTTATCGTTATGCACACTTGACAATACTCTTTTTTTATTTATTTTTTATTGTGCACTTGTAGTAGAGTATGCAAACAAGAGTGTAGCTCAAGATGTCAAACGCTTAAACTATGGTTGAAACAAAGAGAAGGAAGAAGAGAGCAATTCTCTCTCTCTCTCTCTTAATGAGTCATTCTAAATTTTTGGAATTGAAAGAGTATATACATAAAAGTTGTCTCTCCTTCTCTACTAATAGGTGGGAGCAGAGTTTATAGGCACTTTGATTCTCATATTTGCCGGAACTGCCACCGCCATTGTGAACCAAAAGACGGGAGGAACTGAAACGTTGATCGGCCTGGCGGCTTCCACCGGCCTTGCAGTGATGATCGTGATCTTGTCAACGGGTCACATCTCCGGAGCCCATCTGAATCCAGCTGTCACCATTGCTTTTGCTGCATTGAAGCAGTTTCCTTGGAAGCATGTATGTAACCATTATCAATGTTTTTACATACCGTCACTTATCCGCTTATGTTTTAAAACTTGTTTGAATTCTCTTGATATATATGTATACATATGTTTGCAGGTGCCATTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTTCATCATTTGCATTGAAATGGATCTTTGATCCAATAATGGGAGGAGGAGCCACTATTCCTTCATGTGGATATGCCCAAGCTTTTGCTCTTGAGTTTATTATTAGCTTCAACCTCATGTTTGTCCTCACCGCGGTCGCTACGGACACTCGAGCTGTGAGTTCCTCTTTTCCTTGGGTTTAGATTACTTAGTAAGGATTTTAATTTACACTACTTATCTAGAATTTGTATACCAATATACTGTGTTGTATCATTTGAGTTACTTCTAAATTTATATATTTTATTACAATTAAGTTTGATGTTCGTTCTAATAAATAATAAAGATTAACAAAATGAACTAAAATGTTTATAAAATATCTATATACCGAGATAAACTACGATTATATCCGTCTCTATATCAAAATGTTTATAAAATATCTATATACCGAGATAAACTACGATTATATCCGTCTCTATTGATATTTTGACTTCTTGAAAAGTTTTTGTAATTGTTTTGCTTAATAAAGAAATAAATGAAAACGCAGGTGGGAGAGTTGGCGGGAATCGCGGTAGGGGCAACGGTTATGCTCAACGTACTCATCGCGGGGTATGTACAAAACCAAAACTATATCTCCAATTGTTTCAAATTCTTACATTTTTTTATAATTTAAATCTATATTTACCTTGGATAGATTTTAAATTTTCATTGTAATTATAAATATTTTCGAACACATTTTTCATCCGAAATAATTTAATTCTAAAATGTTCAAATTTATTTCATGATTTAAATTTTATTTCAATTGGGTATCTATGGATAGTTTTAAAATTTTCACTTCAACCTTTAATATTTGAAATTTGTTTCAGTTTCAAAGTATAAAACCGATGTTGCATAATATAGTTTAAAGAGTAAATTGGAATGTATCTTAATTAGTAGTTTTAAAATATTGTTAACAATTTAAAATTATAGATTAAATTGTTACAGAATTGAAAGTATAGAGATTTAATAATTACAAACCAAACCGGTAATGATTCATCCAACTCATAGTAATAGATTTCTCTTCAAAATGTAGACGTGAACAAAGTGGCTACTGTTTCAGAAAGGAAACCGTAATTTAGAGCCTTATCTTATGAACAAATGTTACCCTATATACCTCCATTAAACAACAAACAAACTAAAAAGCTTTTCATTTTAATTATGTAACAGACAAACAACTGGAGCTTCAATGAATCCAGTAAGAACACTGGGGCCAGCCATTGCAGTAAACAATTTCAAAGCCATATGGATTTACCTCACAGCTCCCATATTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCTGACAAAGATGGTGATTCACGCTTGCCTTCTACTGCAGCAAGCTTTCGACGATGACAACTGATCGATCCCCCAACCTACCTACCTAATTAACCCAGAGAACATGTTGAACCTTAGATCCTTGAGACAAATTCTAAATCAGAACGATGGATGAGATGGAAAAATGAAACCAAATGTATGTTCAGTATCAGTAGTAAATACGCAACAGAACAATGTGTATGGATAGGCTGCAAGTATTGGTCATTGTGTGTATGATAGTTAACCAAAGGATTTCCAGTTCTTAACCAAAAGGAAAAGAAACTGCCTAATAATTGTCTTTGTAATCTAGAAATGAAACTATAGCAGTTGAATTGTAAACTCAATTCAGGTAGTAACTAGAAAACCATGGCCTATCTCCCTAACCATTTGATTTCTAGTTTTTTGAAATCCTTTTTATGAATGTTTCCAAGTCATAACCCAAATTTTAAGAACTACAAAC

mRNA sequence

CCAAAGCCAAAAACAAAACAATCTTTATTAATCTTGTTTTTTTCATAGCCTTCTCTCTCTTTTGGAACTTTTGTGCTATGGATACAGAGGAAGCTCCATCAACACCAGTGACGCCGGGAACTCCTGGAGCTCCGCTGTTTGGACGAGTGAAAGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGTTGCATCTCTTTCAATGTTGATGATAATTGGGGGAGTGAAGAAGGTGGCTTAACTAAAATAGTCTCTTCTTGTTCATTGCCATACTCTCCTGTCTCACTTGCAAGAAAGGTGGGAGCAGAGTTTATAGGCACTTTGATTCTCATATTTGCCGGAACTGCCACCGCCATTGTGAACCAAAAGACGGGAGGAACTGAAACGTTGATCGGCCTGGCGGCTTCCACCGGCCTTGCAGTGATGATCGTGATCTTGTCAACGGGTCACATCTCCGGAGCCCATCTGAATCCAGCTGTCACCATTGCTTTTGCTGCATTGAAGCAGTTTCCTTGGAAGCATGTGCCATTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTTCATCATTTGCATTGAAATGGATCTTTGATCCAATAATGGGAGGAGGAGCCACTATTCCTTCATGTGGATATGCCCAAGCTTTTGCTCTTGAGTTTATTATTAGCTTCAACCTCATGTTTGTCCTCACCGCGGTCGCTACGGACACTCGAGCTGTGGGAGAGTTGGCGGGAATCGCGGTAGGGGCAACGGTTATGCTCAACGTACTCATCGCGGGACAAACAACTGGAGCTTCAATGAATCCAGTAAGAACACTGGGGCCAGCCATTGCAGTAAACAATTTCAAAGCCATATGGATTTACCTCACAGCTCCCATATTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCTGACAAAGATGGTGATTCACGCTTGCCTTCTACTGCAGCAAGCTTTCGACGATGACAACTGATCGATCCCCCAACCTACCTACCTAATTAACCCAGAGAACATGTTGAACCTTAGATCCTTGAGACAAATTCTAAATCAGAACGATGGATGAGATGGAAAAATGAAACCAAATGTATGTTCAGTATCAGTAGTAAATACGCAACAGAACAATGTGTATGGATAGGCTGCAAGTATTGGTCATTGTGTGTATGATAGTTAACCAAAGGATTTCCAGTTCTTAACCAAAAGGAAAAGAAACTGCCTAATAATTGTCTTTGTAATCTAGAAATGAAACTATAGCAGTTGAATTGTAAACTCAATTCAGGTAGTAACTAGAAAACCATGGCCTATCTCCCTAACCATTTGATTTCTAGTTTTTTGAAATCCTTTTTATGAATGTTTCCAAGTCATAACCCAAATTTTAAGAACTACAAAC

Coding sequence (CDS)

ATGGATACAGAGGAAGCTCCATCAACACCAGTGACGCCGGGAACTCCTGGAGCTCCGCTGTTTGGACGAGTGAAAGAAAATCAGCATGGAAGTGAGAATGGTAAAAGATCTCTTCTCAAAAGTTGCATCTCTTTCAATGTTGATGATAATTGGGGGAGTGAAGAAGGTGGCTTAACTAAAATAGTCTCTTCTTGTTCATTGCCATACTCTCCTGTCTCACTTGCAAGAAAGGTGGGAGCAGAGTTTATAGGCACTTTGATTCTCATATTTGCCGGAACTGCCACCGCCATTGTGAACCAAAAGACGGGAGGAACTGAAACGTTGATCGGCCTGGCGGCTTCCACCGGCCTTGCAGTGATGATCGTGATCTTGTCAACGGGTCACATCTCCGGAGCCCATCTGAATCCAGCTGTCACCATTGCTTTTGCTGCATTGAAGCAGTTTCCTTGGAAGCATGTGCCATTGTATATTGGAGCTCAAATGGTGGCTTCTCTGTGTTCATCATTTGCATTGAAATGGATCTTTGATCCAATAATGGGAGGAGGAGCCACTATTCCTTCATGTGGATATGCCCAAGCTTTTGCTCTTGAGTTTATTATTAGCTTCAACCTCATGTTTGTCCTCACCGCGGTCGCTACGGACACTCGAGCTGTGGGAGAGTTGGCGGGAATCGCGGTAGGGGCAACGGTTATGCTCAACGTACTCATCGCGGGACAAACAACTGGAGCTTCAATGAATCCAGTAAGAACACTGGGGCCAGCCATTGCAGTAAACAATTTCAAAGCCATATGGATTTACCTCACAGCTCCCATATTAGGGACATTGTGTGGAGCTGGAATCTACACTGCAGTTAAGTTGCCTGACAAAGATGGTGATTCACGCTTGCCTTCTACTGCAGCAAGCTTTCGACGATGA

Protein sequence

MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMGGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAASFRR*
Homology
BLAST of CSPI04G02100 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.4e-114
Identity = 222/310 (71.61%), Postives = 248/310 (80.00%), Query Frame = 0

Query: 1   MDTEEAPSTP----VTPGTPGAPLFG--RVKENQHGSENGKRSLLKSCISFNVDDNWGSE 60
           MD EE PSTP     TPGTPGAPLFG    K N H      +SLLKSC  F+VD+ W  E
Sbjct: 1   MDHEEIPSTPSTPATTPGTPGAPLFGGFEGKRNGHNGRYTPKSLLKSCKCFSVDNEWALE 60

Query: 61  EGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAAS 120
           +G L  +  +CSLP   VSL RK+GAEF+GTLILIFAGTATAIVNQKT G ETLIG AAS
Sbjct: 61  DGRLPPV--TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAAS 120

Query: 121 TGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWI 180
            GLAVMIVILSTGHISGAHLNPAVTIAFAALK FPWKHVP+YIGAQ++AS+ ++FALK +
Sbjct: 121 AGLAVMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAV 180

Query: 181 FDPIMGGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNV 240
           F+P M GG T+P+ G +QAFALEFIISFNLMFV+TAVATDTRAVGELAGIAVGATVMLN+
Sbjct: 181 FEPTMSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNI 240

Query: 241 LIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSR 300
           LIAG  T ASMNPVRTLGPAIA NN++AIW+YLTAPILG L GAG YT VKLP++D    
Sbjct: 241 LIAGPATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED---E 300

Query: 301 LPSTAASFRR 305
            P    SFRR
Sbjct: 301 APKERRSFRR 305

BLAST of CSPI04G02100 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.1e-90
Identity = 179/299 (59.87%), Postives = 212/299 (70.90%), Query Frame = 0

Query: 6   APSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTKIVSSC 65
           AP TP TPGTPG PL   ++ +    ++ K +    C+   +   WG  +   T      
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPV-MGSTWGQHDTCFT------ 74

Query: 66  SLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILS 125
             P   VSL RK+GAEF+GT ILIF  TA  IVNQK  G ETLIG AA  GLAVMI+ILS
Sbjct: 75  DFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILS 134

Query: 126 TGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMGGGATI 185
           TGHISGAHLNP++TIAFAAL+ FPW HVP YI AQ+ AS+C+SFALK +F P M GG TI
Sbjct: 135 TGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTI 194

Query: 186 PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGASM 245
           PS    QAFALEFII+F L+FV+TAVATDTRAVGELAGIAVGATVMLN+L+AG +TG SM
Sbjct: 195 PSVSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSM 254

Query: 246 NPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAASFRR 305
           NPVRTLGPA+A  N++++W+YL AP LG + GA +YT VKL D   D   P    SFRR
Sbjct: 255 NPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDP--PRPVRSFRR 304

BLAST of CSPI04G02100 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 3.1e-82
Identity = 169/299 (56.52%), Postives = 204/299 (68.23%), Query Frame = 0

Query: 8   STPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTKIVSSCSL 67
           S P TPGTP APLF   + +    E       K C+   V + W     G         +
Sbjct: 22  SAPATPGTP-APLFAGPRVDSLSYERKSMPRCK-CLPAAVAEAWAPSAHGCV-----VEI 81

Query: 68  PYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILSTG 127
           P   VSL RK+GAEF+GT ILIF  TA  IVNQK GG  +  G AA  GLAV  +ILSTG
Sbjct: 82  PAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTG 141

Query: 128 HISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMGGGATI-- 187
           HISGAHLNP++TIAFAAL+ FPW  VP Y+  Q++ S+C+ FALK +F P + GG T+  
Sbjct: 142 HISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQVLGSICAGFALKGVFHPFLSGGVTVPD 201

Query: 188 PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGASM 247
           P+   AQAF  EFII+FNL+FV+TAVATDTRAVGELAGIAVGA V LN+LIAG TTG SM
Sbjct: 202 PTISTAQAFFTEFIITFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILIAGPTTGGSM 261

Query: 248 NPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAASFRR 305
           NPVRTLGPA+A  N++ +WIYL AP LG + GAG+YTAVKL D++G++  P    SFRR
Sbjct: 262 NPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAGVYTAVKLRDENGET--PRPQRSFRR 311

BLAST of CSPI04G02100 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.3e-82
Identity = 169/300 (56.33%), Postives = 205/300 (68.33%), Query Frame = 0

Query: 8   STPVTPGTPGAPLFGRVKENQHGSENGKRSLLK-SCISFNVDDNWGSEEGGLTKIVSSCS 67
           S P TPGTP APLF             ++S+ +  C+     + W     G+        
Sbjct: 11  SAPATPGTP-APLFSSGGPRVDSLSYERKSMPRCKCLPLPAVEGW-----GVATHTCVVE 70

Query: 68  LPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILST 127
           +P   VSL RK+GAEF+GT ILIF  TA  IVNQK GG  +  G AA  GLAV  VILST
Sbjct: 71  IPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVATVILST 130

Query: 128 GHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMGGGATIP 187
           GHISGAHLNP++TIAFAAL+ FPW  VP Y+  Q +AS+C++FALK +F P + GG T+P
Sbjct: 131 GHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQALASVCAAFALKGVFHPFLSGGVTVP 190

Query: 188 --SCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGAS 247
             +   AQAF  EFIISFNL+FV+TAVATDTRAVGELAGIAVGA V LN+L+AG TTG S
Sbjct: 191 DATVSTAQAFFTEFIISFNLLFVVTAVATDTRAVGELAGIAVGAAVTLNILVAGPTTGGS 250

Query: 248 MNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAASFRR 305
           MNPVRTLGPA+A  N++ +WIYL AP LG L GA +Y AVKL D++G++  P T  SFRR
Sbjct: 251 MNPVRTLGPAVAAGNYRQLWIYLLAPTLGALAGASVYKAVKLRDENGET--PRTQRSFRR 302

BLAST of CSPI04G02100 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 124/243 (51.03%), Postives = 163/243 (67.08%), Query Frame = 0

Query: 53  SEEGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLA 112
           SE+ G +K  SS + P   V  A+KV AE +GT  LIFAG A   VN++TGGT T  G+ 
Sbjct: 32  SEQDGGSK-SSSNNHPMFSVQFAQKVIAEILGTFFLIFAGCAAVAVNKRTGGTVTFPGIC 91

Query: 113 ASTGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALK 172
            + GLAVM+++ S GHISGAHLNPAVT+AFA   +FPW+ VP Y  AQ+  S  +S AL+
Sbjct: 92  ITWGLAVMVMVYSVGHISGAHLNPAVTLAFATCGRFPWRRVPAYAAAQVAGSAAASAALR 151

Query: 173 WIFDPIMGGGA------TIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAV 232
            +F     GGA      T P+    Q+ A+EFII+F LMFV++ VATD RA+GELAG+AV
Sbjct: 152 ALF-----GGAPEHFFGTAPAGSDVQSLAMEFIITFYLMFVVSGVATDNRAIGELAGLAV 211

Query: 233 GATVMLNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKL 290
           GATV++NVL AG  +GASMNP RT+GPAI +  +  IW+Y+  P+ G + GA  Y  ++ 
Sbjct: 212 GATVLVNVLFAGPISGASMNPARTIGPAIILGRYTGIWVYIAGPVFGAVAGAWAYNLIRF 268

BLAST of CSPI04G02100 vs. ExPASy TrEMBL
Match: A0A0A0KX08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKW FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT
Sbjct: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. ExPASy TrEMBL
Match: A0A5D3E2S7 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002260 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.3e-160
Identity = 295/304 (97.04%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I FNVDD+WG EEGGL+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. ExPASy TrEMBL
Match: A0A1S3BY36 (aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.3e-160
Identity = 295/304 (97.04%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I FNVDD+WG EEGGL+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. ExPASy TrEMBL
Match: A0A5A7TNN6 (Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002250 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.7e-160
Identity = 294/304 (96.71%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I FNVDD+WG EEGGL+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLN+LIAGQT
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNILIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. ExPASy TrEMBL
Match: A0A6J1KEN1 (aquaporin NIP6-1 OS=Cucurbita maxima OX=3661 GN=LOC111492577 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.0e-136
Identity = 257/304 (84.54%), Postives = 274/304 (90.13%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEE PS PVTPGTPGAPLFG  K N  GSENGKRSLLKSCISFNVDD WG+EEG L+K
Sbjct: 1   MDTEEVPS-PVTPGTPGAPLFGGHKGNHQGSENGKRSLLKSCISFNVDD-WGTEEGSLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
                      VSLARKVGAEFIGTL+LIFAGTATAIVNQK  GTETLIGLAASTG+AVM
Sbjct: 61  -----------VSLARKVGAEFIGTLMLIFAGTATAIVNQKIQGTETLIGLAASTGMAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           +VILSTGHISGAHLNP +TIAFAALK FPWK VP+YIGAQ+VASLC+SFALKWIFDP MG
Sbjct: 121 VVILSTGHISGAHLNPGITIAFAALKHFPWKQVPVYIGAQIVASLCASFALKWIFDPFMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GG T+PSCGY+QAFALEFIISFNLMFV+TAVATDTRAVGELAGI VGATVMLNVLIAG+T
Sbjct: 181 GGVTVPSCGYSQAFALEFIISFNLMFVITAVATDTRAVGELAGIVVGATVMLNVLIAGET 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNF+AIWIYLTAPILGTLCGAGIYTA+KLPD+DGD+RLPSTA 
Sbjct: 241 TGASMNPVRTLGPAIAANNFRAIWIYLTAPILGTLCGAGIYTAIKLPDQDGDARLPSTAR 291

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 291

BLAST of CSPI04G02100 vs. NCBI nr
Match: XP_004152170.1 (aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [Cucumis sativus])

HSP 1 Score: 592.4 bits (1526), Expect = 2.2e-165
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKW FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWTFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT
Sbjct: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. NCBI nr
Match: XP_008454166.1 (PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 575.5 bits (1482), Expect = 2.8e-160
Identity = 295/304 (97.04%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I FNVDD+WG EEGGL+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. NCBI nr
Match: KAA0044488.1 (aquaporin NIP6-1 [Cucumis melo var. makuwa])

HSP 1 Score: 575.1 bits (1481), Expect = 3.6e-160
Identity = 294/304 (96.71%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKS I FNVDD+WG EEGGL+K
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSWICFNVDDSWGIEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           IVSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTETLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLN+LIAGQT
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNILIAGQT 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. NCBI nr
Match: XP_038876197.1 (aquaporin NIP6-1 [Benincasa hispida])

HSP 1 Score: 567.8 bits (1462), Expect = 5.8e-158
Identity = 286/304 (94.08%), Postives = 296/304 (97.37%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEE PSTPVTPGTPGAPLFGRVKEN+HGSENGKRSLLKSCISFNVDDNWG+EEGGL+K
Sbjct: 1   MDTEETPSTPVTPGTPGAPLFGRVKENRHGSENGKRSLLKSCISFNVDDNWGTEEGGLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
           +VSSCSLPY PVSLARKVGAEFIGTLILIFAGTATAIVNQKT GTE LIGLAASTGLAVM
Sbjct: 61  LVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTEMLIGLAASTGLAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           IVILSTGHISGAHLNPA+TIAFAALKQFPWK+VP+YIGAQMVASLCSSFALKW+FDPIMG
Sbjct: 121 IVILSTGHISGAHLNPAITIAFAALKQFPWKYVPVYIGAQMVASLCSSFALKWMFDPIMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GGAT+PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQ 
Sbjct: 181 GGATVPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQA 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNFKA+WIYLTAPILG LCGAGIYTAVKLPDKDGDSRLPSTA 
Sbjct: 241 TGASMNPVRTLGPAIAANNFKAVWIYLTAPILGALCGAGIYTAVKLPDKDGDSRLPSTAG 300

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 304

BLAST of CSPI04G02100 vs. NCBI nr
Match: XP_023524713.1 (aquaporin NIP6-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.8 bits (1283), Expect = 3.3e-137
Identity = 258/304 (84.87%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60
           MDTEE PS PVTPGTPGAPLFG  K N HGSENGKRSLLKSCISFNV+D WG+EEG L+K
Sbjct: 1   MDTEEVPS-PVTPGTPGAPLFGGHKGNHHGSENGKRSLLKSCISFNVND-WGTEEGSLSK 60

Query: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120
                      VSLARKVGAEFIGTL+LIFAGTATAIVNQK  GTETLIGLAASTG+AVM
Sbjct: 61  -----------VSLARKVGAEFIGTLMLIFAGTATAIVNQKIQGTETLIGLAASTGMAVM 120

Query: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMG 180
           +VILSTGHISGAHLNP +TIAFAALK FPWK VP+YIGAQ+VASLC+SFALKWIFDP MG
Sbjct: 121 VVILSTGHISGAHLNPGITIAFAALKHFPWKQVPVYIGAQIVASLCASFALKWIFDPFMG 180

Query: 181 GGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQT 240
           GG T+PSCGY+QAFALEFIISFNLMFV+TAVATDTRAVGELAGIAVGATVMLNVLIAG+T
Sbjct: 181 GGVTVPSCGYSQAFALEFIISFNLMFVITAVATDTRAVGELAGIAVGATVMLNVLIAGET 240

Query: 241 TGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAA 300
           TGASMNPVRTLGPAIA NNF+AIWIYLTAPILGTLCGAGIYTA+KLPD+DGD+RLPSTA 
Sbjct: 241 TGASMNPVRTLGPAIAANNFRAIWIYLTAPILGTLCGAGIYTAIKLPDQDGDARLPSTAR 291

Query: 301 SFRR 305
           SFRR
Sbjct: 301 SFRR 291

BLAST of CSPI04G02100 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 413.3 bits (1061), Expect = 1.7e-115
Identity = 222/310 (71.61%), Postives = 248/310 (80.00%), Query Frame = 0

Query: 1   MDTEEAPSTP----VTPGTPGAPLFG--RVKENQHGSENGKRSLLKSCISFNVDDNWGSE 60
           MD EE PSTP     TPGTPGAPLFG    K N H      +SLLKSC  F+VD+ W  E
Sbjct: 1   MDHEEIPSTPSTPATTPGTPGAPLFGGFEGKRNGHNGRYTPKSLLKSCKCFSVDNEWALE 60

Query: 61  EGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAAS 120
           +G L  +  +CSLP   VSL RK+GAEF+GTLILIFAGTATAIVNQKT G ETLIG AAS
Sbjct: 61  DGRLPPV--TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAAS 120

Query: 121 TGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWI 180
            GLAVMIVILSTGHISGAHLNPAVTIAFAALK FPWKHVP+YIGAQ++AS+ ++FALK +
Sbjct: 121 AGLAVMIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAV 180

Query: 181 FDPIMGGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNV 240
           F+P M GG T+P+ G +QAFALEFIISFNLMFV+TAVATDTRAVGELAGIAVGATVMLN+
Sbjct: 181 FEPTMSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNI 240

Query: 241 LIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSR 300
           LIAG  T ASMNPVRTLGPAIA NN++AIW+YLTAPILG L GAG YT VKLP++D    
Sbjct: 241 LIAGPATSASMNPVRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED---E 300

Query: 301 LPSTAASFRR 305
            P    SFRR
Sbjct: 301 APKERRSFRR 305

BLAST of CSPI04G02100 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 332.8 bits (852), Expect = 2.9e-91
Identity = 179/299 (59.87%), Postives = 212/299 (70.90%), Query Frame = 0

Query: 6   APSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTKIVSSC 65
           AP TP TPGTPG PL   ++ +    ++ K +    C+   +   WG  +   T      
Sbjct: 15  APPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPV-MGSTWGQHDTCFT------ 74

Query: 66  SLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILS 125
             P   VSL RK+GAEF+GT ILIF  TA  IVNQK  G ETLIG AA  GLAVMI+ILS
Sbjct: 75  DFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILS 134

Query: 126 TGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIFDPIMGGGATI 185
           TGHISGAHLNP++TIAFAAL+ FPW HVP YI AQ+ AS+C+SFALK +F P M GG TI
Sbjct: 135 TGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTI 194

Query: 186 PSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGASM 245
           PS    QAFALEFII+F L+FV+TAVATDTRAVGELAGIAVGATVMLN+L+AG +TG SM
Sbjct: 195 PSVSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSM 254

Query: 246 NPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKDGDSRLPSTAASFRR 305
           NPVRTLGPA+A  N++++W+YL AP LG + GA +YT VKL D   D   P    SFRR
Sbjct: 255 NPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDP--PRPVRSFRR 304

BLAST of CSPI04G02100 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-57
Identity = 122/254 (48.03%), Postives = 165/254 (64.96%), Query Frame = 0

Query: 55  EGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAAS 114
           +GG+  ++  C+ P S V L +K+ AE IGT  ++F+G    +VN   GGT T  G+  +
Sbjct: 25  QGGIETVI--CTSP-SIVCLTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVT 84

Query: 115 TGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWI 174
            GL VM++I STGHISGAH NPAVT+ FA  ++FPW  VPLYIGAQ   SL +S  L+ +
Sbjct: 85  WGLIVMVMIYSTGHISGAHFNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLM 144

Query: 175 F----DPIMGGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATV 234
           F    +   G   T P+   A+A   E IISF LMFV++ VATD RAVGELAGIAVG T+
Sbjct: 145 FKVTPEAFFG---TTPADSPARALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTI 204

Query: 235 MLNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDKD 294
           M+NV +AG  +GASMNP R+LGPA+ +  +K IW+Y+  P+LG + G  +Y  ++  DK 
Sbjct: 205 MVNVFVAGPISGASMNPARSLGPALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDK- 264

Query: 295 GDSRLPSTAASFRR 305
              R  + +ASF R
Sbjct: 265 -PLRELTKSASFLR 270

BLAST of CSPI04G02100 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-57
Identity = 125/257 (48.64%), Postives = 164/257 (63.81%), Query Frame = 0

Query: 50  NWGSEEGGLTKIVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLI 109
           N    +GG+   +  CS P S V L +K+ AE IGT  +IF+G    +VN   GGT T  
Sbjct: 20  NCKDSQGGMETAI--CSSP-SIVCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFP 79

Query: 110 GLAASTGLAVMIVILSTGHISGAHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSF 169
           G+  + GL VM++I STGHISGAH NPAVT+ FA  ++FPW  VPLYIGAQ+  SL +S 
Sbjct: 80  GICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASL 139

Query: 170 ALKWIFD--PIMGGGATIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVG 229
            L+ +F+  P    G T P+    QA   E IISF LMFV++ VATD+RA GELAGIAVG
Sbjct: 140 TLRLMFNVTPKAFFGTT-PTDSSGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVG 199

Query: 230 ATVMLNVLIAGQTTGASMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLP 289
            T++LNV +AG  +GASMNP R+LGPAI +  +K IW+Y+  P +G   G  +Y  ++  
Sbjct: 200 MTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFT 259

Query: 290 DKDGDSRLPSTAASFRR 305
           DK    R  + +ASF R
Sbjct: 260 DK--PLRELTKSASFLR 270

BLAST of CSPI04G02100 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 202.2 bits (513), Expect = 5.9e-52
Identity = 110/226 (48.67%), Postives = 153/226 (67.70%), Query Frame = 0

Query: 72  VSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVMIVILSTGHISG 131
           V   +K+ AE +GT  LIFAG A   VN +     TL G+A   GL VM+++ S GHISG
Sbjct: 48  VPFLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISG 107

Query: 132 AHLNPAVTIAFAALKQFPWKHVPLYIGAQMVASLCSSFALKWIF--DPIMGGG------A 191
           AH NPAVTIAFA+  +FP K VP Y+ +Q++ S  ++  L+ +F  D  +  G       
Sbjct: 108 AHFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVG 167

Query: 192 TIPSCGYAQAFALEFIISFNLMFVLTAVATDTRAVGELAGIAVGATVMLNVLIAGQTTGA 251
           T+PS    Q+F +EFII+F LMFV++ VATD RA+GELAG+AVG+TV+LNV+IAG  +GA
Sbjct: 168 TLPSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGA 227

Query: 252 SMNPVRTLGPAIAVNNFKAIWIYLTAPILGTLCGAGIYTAVKLPDK 290
           SMNP R+LGPA+  + ++ +WIY+ +PI+G + GA +Y  V+  DK
Sbjct: 228 SMNPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDK 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAI42.4e-11471.61Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1[more]
Q9SV844.1e-9059.87Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1[more]
Q0IWF33.1e-8256.52Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2[more]
Q9ATN15.3e-8256.33Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1[more]
Q0DK161.9e-5851.03Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KX081.1e-16599.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1[more]
A0A5D3E2S71.3e-16097.04Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002... [more]
A0A1S3BY361.3e-16097.04aquaporin NIP6-1 OS=Cucumis melo OX=3656 GN=LOC103494651 PE=3 SV=1[more]
A0A5A7TNN61.7e-16096.71Aquaporin NIP6-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0022... [more]
A0A6J1KEN11.0e-13684.54aquaporin NIP6-1 OS=Cucurbita maxima OX=3661 GN=LOC111492577 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152170.12.2e-16599.67aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [... [more]
XP_008454166.12.8e-16097.04PREDICTED: aquaporin NIP6-1 [Cucumis melo] >TYK29615.1 aquaporin NIP6-1 [Cucumis... [more]
KAA0044488.13.6e-16096.71aquaporin NIP6-1 [Cucumis melo var. makuwa][more]
XP_038876197.15.8e-15894.08aquaporin NIP6-1 [Benincasa hispida][more]
XP_023524713.13.3e-13784.87aquaporin NIP6-1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G80760.11.7e-11571.61NOD26-like intrinsic protein 6;1 [more]
AT4G10380.12.9e-9159.87NOD26-like intrinsic protein 5;1 [more]
AT5G37810.11.2e-5748.03NOD26-like intrinsic protein 4;1 [more]
AT5G37820.11.6e-5748.64NOD26-like intrinsic protein 4;2 [more]
AT4G18910.15.9e-5248.67NOD26-like intrinsic protein 1;2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 115..139
score: 50.51
coord: 76..95
score: 40.51
coord: 152..171
score: 25.13
coord: 227..249
score: 40.24
coord: 196..214
score: 25.04
coord: 264..284
score: 31.1
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 72..281
e-value: 4.2E-48
score: 164.0
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 65..293
e-value: 3.1E-64
score: 218.3
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 72..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR45724:SF41BNAA02G36290D PROTEINcoord: 6..292
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 6..292
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 133..141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G02100.1CSPI04G02100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity