CSPI04G02040 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G02040
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDefective in cullin neddylation protein
LocationChr4: 1166479 .. 1170620 (+)
RNA-Seq ExpressionCSPI04G02040
SyntenyCSPI04G02040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAACCTAACTACATACATTATAATATAATTTAAGAAAACCAAGAAATAAAAAAGGGGAAAAAATTAAAAAGACGAAAGGGCCGAAGATTTGCAATTCTCCAATTTGCTGCGACAAAATTCAATCTTCTTCTTCTTCTTCTTCTTATTTGCTCTCTCTTCTTATCTCCTTCATCGCAATCCAATTCTTCAAGAACAAGAACAAGAAGATGCGGCGGTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTCGATCTCTTTCGCTCCGGTACTTGATCTTTTATTTTTTCCACAATTCCAATTCATCTACTGCTAAATCTCGCCATAGACATCGATGATCGTCTTTTATATGTATTGTGGTTCATTTTTTATTAATTACTGTTATGATGATGCCTTTGTATAGGAGGTTTTTATGTTTACCATTTTATGTGCGCATCTTGTTATGTTGATGGTTTAGTTAGTTTTTAAATCGAGTGGGATTTAAGTAGATGAATAGATTTAGTATTTAGCTTCAATTGTGTTCACGCGCTATGAAATTTGGGAATTTGGAGCGTGTGTTATGATTTTTGAGTTACATGTACTTGAGGACCTGTTGGAGTTAATCCGAGATGATATGGAGTGAGTTGAAGGTTTGATTGGAAAGTGAGAGTTGTCGAGTTGGAAATTGAAAGCAAGAGAATCTGAACATGCAAACTATTTTTATACATTGTTTTCAGTTTTTTTTTCCACATTTAATTGAGTGAAGAATGATGAACTTTGTTGGGTGATCTATTGGCTGTTTTTAAGAAAAACTGTGTGGTTCGTGGCTTTCTTAGTCATATGGTAGTAACTAGTAAGTATCATGCTGTTTTGTTCTTATGAGGTGATTTATGTGAAAGTGTCTGATTCTTTTTAAATCTTCCACAATATAAGCTCCTATTTTTACTCTATTTTCAATTCTTATGAACTCGATTCTCAATTTATTCAGCCTCAAGTAGAGCAAGCTCAAAAGAGTTGGAACGGATCGATAGCCTATTTTACTCGTATGCCAATCCCTCTTCTGGTTTGATCGAGTAAGTCAACTCTGGTCAATTGAAATATCTAGTTTTTCAGCATTGTTAGTTAGCTTTGCGTTGTATCATATATATTCAGTATTGTAATGTTAAATTCTTAGCAATATTCCAATAACAAACTTCCATCCATGTTCTTTACATGATCAAGGAGGCTATTCTCCTTATCATTAAGGACACTTCTGAGTGCTTGTATGAATAAGATCCTTATAGGTGTACGTAGTACTGACTATTTCTCTTTAACAGTCCAGAAGGAATTGAAAATCTTTGTTCAGACATTGAAGTGGACCACACTGATGTAAGGATCTTGATGCTTGCTTGGTAAGTAGTACTTGGAAGTATTGAAACACTTCTTTCTCCTTTTTTCTTTTGGTTTATATGTCCTCATTGTCTGGGAACTAATGGATTGCACATACCAGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGTAAGAATATTCACTCTCTTGTGGACCTAGGATCATTGTAAATTATACAATGGATCTTCTTTCTAGTATTACTTGAACCTTTGTTGTCCCAAACATTTTTTTTTGTTGATATTCTGTCCTTTTATCTACCACAGTATGCTTCACATCATATAATCCACTCTTTCATTTTCTAACTAGTATATTAATCCACGAAGAGTATATTTTATTTTATCCAAGTATTGAGTCTTTATTATCTCCTTTAGAGTGGGGTTCTAACCCCCATTCTTGTGTAATTTTATACCACCACGGAAATTGTTTTTTATTTTAAAAAACTTTGATTTTATCTGTGTTCAGCACCAGCTTATAAGGAAAATCATGTCATTTATATTCTCAGCTTCAATTTAGAAATTTTGGTTGCTAAGAATACCTATGAATTGTTTATGTTTTTTATGTTACTCGTTTATTTTTCTCATCTTTTCTTCATGTCCACTTCCCATCTTATTACAAGATAAAGCATTTATTCCCTCCTTGGTAAATTCATTTGACGGAACATTAACCTGGTACATATTTGATAGGATGAATGGCGAACAGGTCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTATTATACTTGTCTTGTTGAAACTTTCAAAATAAATTATCTAGTGTATTGCATAATTTTGTGCACAAAGTTTTTAGTAACCAATTTCTGACGTTTTGAGAAGGTAAGGAGGCCATCAAACTTTGTGGACTTCTATTCTTATGCATTCCGGTATTGTTTAACAGGTAAATAAATAATGTTTAGTGTATTTTTAGTCTTGAAGCTTGCTTCAATTTGATCCTTACGTTCTCTTTTTCCATTGATATTTTATTTAGAGGAGAAGCAAAAGAGTATTGACATAGAAAGTATATGCGAGTTACTGGATCTTGTTTTGGGGTCTCAGTTTCATTCCCAAGTCAATGCATTTGTTGATTACTTGAAGGTGAGCACTACCTCATTTTTTTGTCTTTTAGTTTGCCACATTTATCCACAGGCATGGTCTGTTTGACACTTCACACCGCAGTTAACTCCAGCACTGCTTAACAGTACTCCTGGGTCTTCTATAGTAGCACTAGCACTTAGTCTAAGAGTTGCATTCTTGAGTTATAGCCTCTCGCCAGCATACCTATTGGAGCACATTGCCAAGTAGTGATAAATGGACTTGAAGGACCTAGTTAATCTTTCTTCTCACGAGAACCTTAGGAGTTATTACATGGTTGGCAATTTTGTGAAAGACATGTTTCCGAGTAACAAATTTTCTTGCATAGGATTTCAACCTTTTGCATTTATTGACCGATTGTTTCCATGTTTTCTGTCTTTCTCCCTCATCAAGAAGGTCAAAAGAAAATTTCTTATTTGCCATATTACATATGCAGATTCAAACTGACTATAAAGTTATTAACATGGATCAATGGATGGGCTTCTTTCGTTTCTGCAATGAGGTACCTTCATTATCCCCCAAAGCACATTAACACCGACATGTGTCATTATATTTGCTTGCATATATCGCACTACCAGTGAAATACAGTGAGGAAAATAGTTCTGGAATCAACTTATGAACATGCTCTGAATTCTACAAGAGAGTCCGTGCAAATTCAATGATACGCTGATTCCTTGAATTGAATAAAACAAGTAGGGTCATCAAAACAATAATAATAATAAAAGAAAAAAAAACAAGTAGAATCATCAAAATAAAACTAAAAAAGAACTGTCACTTTTCCCCTATTGAACCTATAGATATGGAAAATAAACAAATTTCTAATTGAAATAATCTAGAAAAAAGGATCCACCTTAAATTTCTTTGTTTAGTATCGTCCCCATAAACTATTGATAAATCCAATCCTTGACGCTTGATGATGACTATGCAGATAAGTTATCCAGACTTGATCAATTACGATTCGGAACTCGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAATTAGAAACCTGATACAATGTATTTTGATGTATACGCCACGGTTGTTGAAGCAAGTATCCTATTAATTTCTCATACCATTATGGATTCGATTCAATATATTTTCCTCGTCATTACTAACACTCTTTATGAACATTTCTGTTTCTACAGGCTCACCTTCGATTACACGTTTGGATAGCAGCAGGATTTTCACTCATTATTTCTTCTTATTTTACTCTTCTTTTTTCTTTAAAAAAAAAAAGAAAATTAACTTCTTCGAAATCAATGGTCATTCCCATTATTATGTTCAAATTATCATTTCAAACATTCATGGATTATTCTTCTGCTAAATTATTGAAGTTTCTGCCTCTGCTTTTCGTGGCCATTCCATTCTTCATCTTCTTACTTAGCCACTCCTTTGATATTGACAGTCTTTTTAGGTATCCATGAAACAGCAGCTCAAGTCTGGATAACAAAAAGAGAAAGTTTGTAAAAACTTTAATATCGATGTCGAATCCTTTCATTTTTGGATATGTTGAATCCTTTACTATCGATATAATCCTTTAATATCGATCTTCATCCTTTCATATCATTTATTTATATATGGCAAAAATTGTGCAAT

mRNA sequence

GTAAAACCTAACTACATACATTATAATATAATTTAAGAAAACCAAGAAATAAAAAAGGGGAAAAAATTAAAAAGACGAAAGGGCCGAAGATTTGCAATTCTCCAATTTGCTGCGACAAAATTCAATCTTCTTCTTCTTCTTCTTCTTATTTGCTCTCTCTTCTTATCTCCTTCATCGCAATCCAATTCTTCAAGAACAAGAACAAGAAGATGCGGCGGTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTCGATCTCTTTCGCTCCGCCTCAAGTAGAGCAAGCTCAAAAGAGTTGGAACGGATCGATAGCCTATTTTACTCGTATGCCAATCCCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAAATCTTTGTTCAGACATTGAAGTGGACCACACTGATGTAAGGATCTTGATGCTTGCTTGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGATGAATGGCGAACAGGTCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCATCAAACTTTGTGGACTTCTATTCTTATGCATTCCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAAAGTATATGCGAGTTACTGGATCTTGTTTTGGGGTCTCAGTTTCATTCCCAAGTCAATGCATTTGTTGATTACTTGAAGATTCAAACTGACTATAAAGTTATTAACATGGATCAATGGATGGGCTTCTTTCGTTTCTGCAATGAGATAAGTTATCCAGACTTGATCAATTACGATTCGGAACTCGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAATTAGAAACCTGATACAATGTATTTTGATGTATACGCCACGGTTGTTGAAGCTCACCTTCGATTACACGTTTGGATAGCAGCAGGATTTTCACTCATTATTTCTTCTTATTTTACTCTTCTTTTTTCTTTAAAAAAAAAAAGAAAATTAACTTCTTCGAAATCAATGGTCATTCCCATTATTATGTTCAAATTATCATTTCAAACATTCATGGATTATTCTTCTGCTAAATTATTGAAGTTTCTGCCTCTGCTTTTCGTGGCCATTCCATTCTTCATCTTCTTACTTAGCCACTCCTTTGATATTGACAGTCTTTTTAGGTATCCATGAAACAGCAGCTCAAGTCTGGATAACAAAAAGAGAAAGTTTGTAAAAACTTTAATATCGATGTCGAATCCTTTCATTTTTGGATATGTTGAATCCTTTACTATCGATATAATCCTTTAATATCGATCTTCATCCTTTCATATCATTTATTTATATATGGCAAAAATTGTGCAAT

Coding sequence (CDS)

ATGCGGCGGTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTCGATCTCTTTCGCTCCGCCTCAAGTAGAGCAAGCTCAAAAGAGTTGGAACGGATCGATAGCCTATTTTACTCGTATGCCAATCCCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAAATCTTTGTTCAGACATTGAAGTGGACCACACTGATGTAAGGATCTTGATGCTTGCTTGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGATGAATGGCGAACAGGTCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCATCAAACTTTGTGGACTTCTATTCTTATGCATTCCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAAAGTATATGCGAGTTACTGGATCTTGTTTTGGGGTCTCAGTTTCATTCCCAAGTCAATGCATTTGTTGATTACTTGAAGATTCAAACTGACTATAAAGTTATTAACATGGATCAATGGATGGGCTTCTTTCGTTTCTGCAATGAGATAAGTTATCCAGACTTGATCAATTACGATTCGGAACTCGCTTGGCCGTTGATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAATTAG

Protein sequence

MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN*
Homology
[Close]
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match: Q9BTE7 (DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-33
Identity = 70/186 (37.63%), Postives = 112/186 (60.22%), Query Frame = 0 

Query: 44  FYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSL 103
           FY YA P   ++ PEG+E  C DI V+  ++ +L+LAWK+++E  G+F  +EW  G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 223
            +    + F  YL+ Q+ Y+V+N DQW     F   + + DL NYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQ 230
           W + +Q
Sbjct: 235 WQKVRQ 235

[Close]
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match: Q1RMX9 (DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.7e-33
Identity = 70/186 (37.63%), Postives = 112/186 (60.22%), Query Frame = 0 

Query: 44  FYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSL 103
           FY YA P   ++ PEG+E  C DI V+  ++ +L+LAWK+++E  G+F  +EW  G+ SL
Sbjct: 54  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 113

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 114 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 173

Query: 164 QFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 223
            +    + F  YL+ Q+ Y+V+N DQW     F   + + DL NYD + AWP++LD FVE
Sbjct: 174 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 233

Query: 224 WLQAKQ 230
           W + +Q
Sbjct: 234 WHKVRQ 234

[Close]
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match: Q9CXV9 (DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.7e-33
Identity = 70/186 (37.63%), Postives = 112/186 (60.22%), Query Frame = 0 

Query: 44  FYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSL 103
           FY YA P   ++ PEG+E  C DI V+  ++ +L+LAWK+++E  G+F  +EW  G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQSRFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 223
            +    + F  YL+ Q+ Y+V+N DQW     F   + + DL NYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQ 230
           W + +Q
Sbjct: 235 WQKIRQ 235

[Close]
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match: Q5PPL2 (DCN1-like protein 5 OS=Rattus norvegicus OX=10116 GN=Dcun1d5 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.7e-33
Identity = 70/186 (37.63%), Postives = 112/186 (60.22%), Query Frame = 0 

Query: 44  FYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSL 103
           FY YA P   ++ PEG+E  C DI V+  ++ +L+LAWK+++E  G+F  +EW  G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQSRFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 223
            +    + F  YL+ Q+ Y+V+N DQW     F   + + DL NYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQ 230
           W + +Q
Sbjct: 235 WQKIRQ 235

[Close]
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match: Q5RDF9 (DCN1-like protein 5 OS=Pongo abelii OX=9601 GN=DCUN1D5 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 69/186 (37.10%), Postives = 111/186 (59.68%), Query Frame = 0 

Query: 44  FYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSL 103
           FY YA P   ++ PEG+E  C DI V+  ++ +L+LAWK+++E  G+F  +EW  G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 223
            +    + F  Y + Q+ Y+V+N DQW     F   + + DL NYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYPE-QSKYRVMNKDQWYNVLEFSRAV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQ 230
           W + +Q
Sbjct: 235 WQKVRQ 235

[Close]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match: A0A0A0KYL5 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G006480 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI
Sbjct: 92  MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 151

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 152 ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 211

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT
Sbjct: 212 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 271

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN
Sbjct: 272 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 321

[Close]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match: A0A1S4DZM9 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 8.1e-126
Identity = 226/230 (98.26%), Postives = 229/230 (99.57%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSLFYSYANP+SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLKIQT
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWLQAKQN
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

[Close]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match: A0A6J1DKL7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111020935 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 5.6e-119
Identity = 214/230 (93.04%), Postives = 224/230 (97.39%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSL+YSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLK+Q+
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

[Close]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match: A0A6J1GVA7 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457515 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.6e-116
Identity = 210/230 (91.30%), Postives = 224/230 (97.39%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSAT+KTGQPNS SVNSSAVDLFRSASSRASSKELERIDSLFYSYAN SSGLIDPEGI
Sbjct: 1   MRRSATKKTGQPNSASVNSSAVDLFRSASSRASSKELERIDSLFYSYANGSSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+ TDVRILMLAWKMKSEKQGYF L+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNPTDVRILMLAWKMKSEKQGYFTLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRR SNFVDFYSYAFRYCLTE+KQKSIDIESICELLDLVLGS+FH+QV+AF++YLKIQ+
Sbjct: 121 EVRRLSNFVDFYSYAFRYCLTEDKQKSIDIESICELLDLVLGSEFHAQVHAFIEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEIS+PDL NYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 230

[Close]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match: A0A6J1G9W7 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111452134 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.4e-116
Identity = 207/228 (90.79%), Postives = 222/228 (97.37%), Query Frame = 0 

Query: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGIEN 62
           R  TRKTGQPNSTSVNSSAVDLFRSASS+AS+KELERI+SLFYSYAN SSGLIDPEGIE 
Sbjct: 2   RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIET 61

Query: 63  LCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEKEV 122
           LCSD+EVDHTDVRILMLAWKMK+EKQGYFNL+EWR GLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 62  LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 121

Query: 123 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQTDY 182
           RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FV+YLKIQ+DY
Sbjct: 122 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 181

Query: 183 KVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           KVINMDQWMGFFRFCNEI++PDL NYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 182 KVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

[Close]
BLAST of CSPI04G02040 vs. NCBI nr
Match: XP_004152174.1 (DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014584 [Cucumis sativus])

HSP 1 Score: 465.7 bits (1197), Expect = 2.3e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 230

[Close]
BLAST of CSPI04G02040 vs. NCBI nr
Match: XP_008454171.1 (PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-like protein 4 [Cucumis melo])

HSP 1 Score: 459.5 bits (1181), Expect = 1.7e-125
Identity = 226/230 (98.26%), Postives = 229/230 (99.57%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSLFYSYANP+SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLKIQT
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWLQAKQN
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

[Close]
BLAST of CSPI04G02040 vs. NCBI nr
Match: XP_038876378.1 (DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 2.8e-120
Identity = 218/230 (94.78%), Postives = 226/230 (98.26%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANASSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFH QV+AFV+YLKIQ+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVDAFVEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEIS+ DL NYDSELAWPLILDNFVEWLQAKQN
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFSDLSNYDSELAWPLILDNFVEWLQAKQN 230

[Close]
BLAST of CSPI04G02040 vs. NCBI nr
Match: XP_022153426.1 (DCN1-like protein 4 [Momordica charantia])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 214/230 (93.04%), Postives = 224/230 (97.39%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSL+YSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLK+Q+
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

[Close]
BLAST of CSPI04G02040 vs. NCBI nr
Match: KAG6581963.1 (DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018382.1 DCN1-like protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 429.1 bits (1102), Expect = 2.4e-116
Identity = 211/230 (91.74%), Postives = 224/230 (97.39%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRSATRKTGQPNS SVNSSAVDLFRSASSRASSKELERIDSLFYSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSASVNSSAVDLFRSASSRASSKELERIDSLFYSYANGSSGLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+ TDVRILMLAWKMKSEKQGYF L+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNPTDVRILMLAWKMKSEKQGYFTLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRR SNFVDFYSYAFRYCLTE+KQKSIDIESICELLDLVLGS+FH+QV+AF++YLKIQ+
Sbjct: 121 EVRRLSNFVDFYSYAFRYCLTEDKQKSIDIESICELLDLVLGSEFHAQVHAFIEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 231
           DYKVINMDQWMGFFRFCNEIS+PDL NYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 230

[Close]
BLAST of CSPI04G02040 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 349.0 bits (894), Expect = 3.0e-96
Identity = 166/229 (72.49%), Postives = 200/229 (87.34%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS+KE++RID LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV HTD+RILMLAWKMK+EKQGYF  +EWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FV+YLKIQ 
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQ 230
           DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+Q KQ
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQ 226

[Close]
BLAST of CSPI04G02040 vs. TAIR 10
Match: AT1G15860.2 (Domain of unknown function (DUF298) )

HSP 1 Score: 349.0 bits (894), Expect = 3.0e-96
Identity = 166/229 (72.49%), Postives = 200/229 (87.34%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS+KE++RID LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV HTD+RILMLAWKMK+EKQGYF  +EWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FV+YLKIQ 
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQ 230
           DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+Q KQ
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQ 226

[Close]
BLAST of CSPI04G02040 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 341.3 bits (874), Expect = 6.2e-94
Identity = 166/238 (69.75%), Postives = 200/238 (84.03%), Query Frame = 0 

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS+KE++RID LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV HTD+RILMLAWKMK+EKQGYF  +EWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLK--- 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FV+YLK   
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWI 180

Query: 181 ------IQTDYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQ 230
                 IQ DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+Q KQ
Sbjct: 181 TQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQ 235

[Close]
BLAST of CSPI04G02040 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 122.1 bits (305), Expect = 5.9e-28
Identity = 68/210 (32.38%), Postives = 121/210 (57.62%), Query Frame = 0 

Query: 20  SAVDLFRS-ASSRASSKELERIDSLFYSYANPSSGLIDPEGIENLCSDIEVDHTDVRILM 79
           +A D+F S    R+++ E+ R++ L+  Y +P S +I  EGI  LC+D+EV+  D+  L+
Sbjct: 39  AAFDVFYSQPQPRSNAAEVRRLEELYNRYKDPYSDMILAEGISVLCNDLEVEPQDIVTLV 98

Query: 80  LAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRY 139
           L+W M +     F+  E+ +GL++L  D++ KL++ LP +  E++    F + Y++AF +
Sbjct: 99  LSWHMNAATACEFSRQEFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGW 158

Query: 140 CLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQTDYKVINMDQWMGFFRFCN 199
              E+ QKS+ +++   +  L+   +    V  + D+L+ + + K I+ D W     F  
Sbjct: 159 A-KEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHN-KAISKDTWAQLLEFSR 218

Query: 200 EISYPDLINYDSELAWPLILDNFVEWLQAK 229
            +  P L NYD+E AWP ++D FVE+L  K
Sbjct: 219 MVD-PVLSNYDAEGAWPYLIDEFVEYLYDK 245

[Close]
BLAST of CSPI04G02040 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 51.2 bits (121), Expect = 1.3e-06
Identity = 32/98 (32.65%), Postives = 50/98 (51.02%), Query Frame = 0 

Query: 126 SNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQTDYKVI 185
           + F  FY + F  C  E  QK+I I        LVL  +F   +N + D+++    +  I
Sbjct: 84  TEFTCFYDFVFFMC-RENGQKNITISRAITAWKLVLAGRFR-LLNRWCDFIEKNQRHN-I 143

Query: 186 NMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVE 224
           + D W     F +   + +L  YDSE AWP+++D+FVE
Sbjct: 144 SEDTWQQVLAF-SRCVHENLEGYDSEGAWPVLIDDFVE 177

The following BLAST results are available for this feature:
BLAST of CSPI04G02040 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9BTE75.9e-3337.63DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1[more]
Q1RMX97.7e-3337.63DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1[more]
Q9CXV97.7e-3337.63DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1[more]
Q5PPL27.7e-3337.63DCN1-like protein 5 OS=Rattus norvegicus OX=10116 GN=Dcun1d5 PE=2 SV=1[more]
Q5RDF93.8e-3237.10DCN1-like protein 5 OS=Pongo abelii OX=9601 GN=DCUN1D5 PE=2 SV=1[more]
BLAST of CSPI04G02040 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KYL51.1e-127100.00Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G0064... [more]
A0A1S4DZM98.1e-12698.26Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 ... [more]
A0A6J1DKL75.6e-11993.04Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1GVA72.6e-11691.30Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1G9W73.4e-11690.79Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of CSPI04G02040 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004152174.12.3e-127100.00DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014... [more]
XP_008454171.11.7e-12598.26PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-li... [more]
XP_038876378.12.8e-12094.78DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Ben... [more]
XP_022153426.11.2e-11893.04DCN1-like protein 4 [Momordica charantia][more]
KAG6581963.12.4e-11691.74DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018382... [more]
BLAST of CSPI04G02040 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G15860.13.0e-9672.49Domain of unknown function (DUF298) [more]
AT1G15860.23.0e-9672.49Domain of unknown function (DUF298) [more]
AT1G15860.36.2e-9469.75Domain of unknown function (DUF298) [more]
AT3G12760.15.9e-2832.38CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP... [more]
AT3G28970.11.3e-0632.65Domain of unknown function (DUF298) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042460DCN1-like, PONY binding domainGENE3D1.10.238.200Cullin, PONY binding domaincoord: 125..230
e-value: 2.3E-27
score: 97.3
IPR005176Potentiating neddylation domainPFAMPF03556Cullin_bindingcoord: 110..224
e-value: 2.3E-26
score: 92.6
IPR005176Potentiating neddylation domainPROSITEPS51229DCUN1coord: 35..226
score: 41.532295
NoneNo IPR availableGENE3D1.10.238.10coord: 33..123
e-value: 7.3E-29
score: 101.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR12281:SF12DEFECTIVE IN CULLIN NEDDYLATION PROTEINcoord: 1..229
IPR014764Defective-in-cullin neddylation proteinPANTHERPTHR12281RP42 RELATEDcoord: 1..229
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 34..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G02040.1CSPI04G02040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051443 positive regulation of ubiquitin-protein transferase activity
biological_process GO:0045116 protein neddylation
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0097602 cullin family protein binding
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0032182 ubiquitin-like protein binding