CSPI04G01360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G01360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGDSL esterase/lipase
LocationChr4: 758427 .. 763766 (+)
RNA-Seq ExpressionCSPI04G01360
SyntenyCSPI04G01360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAACATTTTCGAGTAGAATAAAGTGATAGAGAGGGGTCTCTCACTCATGAACATGAATATTTATTTTAATTATTTTCACATTTTAAACGTGGGCTGATCGGGTTTCTGGGATCTTCTTCTCCCATCTTAGAAAATATCTCAACCACTCTCCCTCTATCAATCTCCAGCCTGACGCCCTTCGCCTCACCTCAGACTAGTTGTCGCCGCACGTCCTGCTTCCGGTGTCGTCCTCCCGCTGCCGCCGCTCGCCACATTTTCAGTAGTGGGTAAATTTTGGTGTTTTCTTATAAATTTTTTCTGGAATGTTTTTTAATACCCACTAAGATTTGGTTTGATGGTTTGGATTAAGCCACATTTGGTCAAAATTTATTTGGAGACCGTTACATTTTATTTTGTGTTTCGGAATTAGGGAGCTAAGTTTGATTATGGTTGTGTTAGGGGCTTTGATCCAAGATTACTAAATATTTTGGCTGAATTTCGAGGTAGGTAGCTTTACGCTTGTTGGATATTATATTAAATTGAATGTCCGTGACCAAGAATCCTTGTTGTTGATTTAAGCTTTTGGTGATTTAGGATGATATTCATTTACATACGATAATATGTTGTGAGACCCCTTATGCCATGCACAATGTATGTTAGTTCAATTAGTGACAACTGTCCTCCTACCCGTAGCTATGCACACCATACAAAGGTTTGTGTTCCTTTGGTTCACATGATAACAGTTCACCAGATTGTACATCTCATCTACCGTATGACAGGTTTCATTGTTCACTCAGATGACCTTACATGTTACAATTAGGTTCCACTAGTTACAGACATTCAGTTGCATCTGCTTTCATAGACCGACTCCAGTAGTGGAATTATGAGCAATTCGTGACTTACTGAATATATTTATTATCCTTGCAAATAGTTTCCAAAATACCCTACATTTTGCTAATATTTTGAACAAGAATCCTATTTCTGCATTTAATCCGTCTGGAAGAAATTGATAATACATCATTTTCCATTCTCACAAGGGTTGATTGAAGCGTTGAAATAACATACAATGAGGCCTGAGATCGTTCTGTTTGGGGATTCTATAACTGCACAATCCTTTGGTTTAGGTGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGAAAGGTAGATTTTTTTTTTTTTTTCTTTCTTTCATTTGTATTTTGATTCTAGTTTTTCAAGTTTATGTTTTTGAAATTGGGTTCTTATCTGACCAATTTATAGGCCGATGTCATAGTTCGTGGCTATGGCGGATACAATACTCGGTGGGCTTTATTTTTACTTCATCACATTTTTCCCTTGGTAGGTGCCATACTGCTCTTTCTGTTTCTTGGCTACTTTGATTCTGATGACTCCACGTTTCACTTGAATTTGCTCCATTATGTTCAGTAAGTGGTATATTGGTCATTATTCTCATTTTAGGACTTCTTTTGTAATGCAACTTATTGTCGTAGTTATTTTTAGGAATTTGTATCCATTTCTAATCCCTACAATTGATTTCAGCTTCATCGAGGATAGTGGGGAATTTTAGCTCATTTGGGCTAAACTAGCTAGATCATACAATGAAATCAAAGATGTGATTGAACAACAAAACTAATCCATTGTGTGAAAGATATTGGAATATTGCTGTATGTTTTAATTAATGATGCTGTATACTAAGTTACTAACATGATTGGGAGTTAAAATACGTGCTTGGTGGGATATTGTTCTGAATTCTTGCGTTAAATTAACTGATACAGAACTCAGATTTGCTAGAACATTGCATTATTGGTTCCCTAGATACATTGGATGCAGGATTTAGTGGCCCAGAATCGATTTGCCTTCACCAAAATGCCTCATTACTCGAAGCTCAGTTTACAAGGCATCCCTCCCACAGCAGGAGTTTCCAGCCTTGCTTCCATCCCAAAGTTTGTGTTCTCATGCTTCTTTGAAACCCTAAAGGCTGAATCACTTTTCTTCAAAGGGATTATAGTCCAAATTTTTCAAGTATTTGATAGATATTCTTTGTACTAGCTTAAGTCATGGGTAGAAACATCTTTTTAAGCTTCTGAAAATCATGTCTCTTGTACGTTCTGCATTCTAAATAAGTTCCATTTCTTGTAATGCAGAATTCCCCAAAGGCCCCCATTGTCGTGACAATATTCTTTGGGGCGAATGATGCAGCTGTTTTGGGGAGAACAAGTGAGAAACAACACGTACCTCTTGAAGAGTATAAGTATAATCTTAAAAAAATGGTAAATCATTTGAAGGTATTGACTCCTTCCTTCATGCTTGAAATATTTTCTACCCAAATCTTTAAGACTCATTTTGAAGATTCAAATCAGTATAATGCATCAGTCCATCACTTCTCCGTTAACTAAATGGTGTGACGTTTCTTCTTTGAGCCCAACTCCCTCTCGCTTAGGACTGTTATTAGTAAACGTGCCTCCCATCCTTTTGGGTGGAATTAACAAAAGTTTTAGAAAAAGAACACCATGATGAGGCAAGAAGTCTAGCTGATCCCTAACATCTGTCCAGACTAATGATTTGTCATGGAGTACACTCTAGTTACGTTCAAACCATAATTCTGATAGGAGAGCTTTAACTTTGTTGGCCACTATCTTGCATTCTTTGATGGAGGGTGTCTGGATACCTAGATTATACTCTTTAGAGATGTGAGTTTGCACGAGCCATTTTGTAGTTGTTTCTTTGAGGTGTTCAATTTTTCGCTTTGCCAGTTCTTGGGGTTGCAAAGAGCTGCTCACTCTTTATTCAACCGTCCTTTCATGAGAAAGTGGTTTTTGTGAGCTTCAATGGCAAAGTTATTTTGTAACTATTCTTTCTATGAGTTGTTTGGTACAACTACAAGGTTTATTAATCATGTTTGATTTTAAATGAGAATGTTATCAGATTTTTTAAATGTAGTTATTTGAAAACAAAAGATGGTAGTTCTAAAAAACTTGTTTTTGTTTTTTGAAATTGGCTAAGAATTCAAATGTTCACACAAGAAATATGAAAATCATGAGTAAGTAATGTCGAAAATAAGAGAGGAGTGAGATGAACAACAAATTGGTCTAGATGAAACTAGAATGTGCTGATGCTATATAATATACTAAAGTTTTCTTCTTATACCTTCTAGGTACATGGGCTAAATAAAGAAGACCCAAATAAACAAGATCTGAGGCTGAATAAAAGAAAGGCCCAGCCAAAATAAGAAATGGAGGAATTAAAGTTAACCACTAACTAAATAAGTTGCTAGAGTTTACTAAATATAGTAACAAATTCTATATAAAGAAATACTATAGTAACAAAGTTCAAACCCTAGATCGAGCATCAGATGGTTAGATCTTCATTAGTAAGGATTTATTAGAAAACAAGCCAAAATTTTCGAAAACCAAAGGTTTACCAGATGACACTCAATATAATTTGATTATTGTACAAAAAATATAGTTTAAATTGATTTTAAGGATCACTGACCTATGAAATGGAGGATCTCCCTAATTTTTTGGATTAGTTCCTCTTTATGGCAGATTGTTCTAGTTCTTCTGAAGTTGGTGGAAATCTTCATAAGATCACTATAGAACCTCTTGGTTTTGGCAAATAATCTCCCTGCCATTCTAGAGGATGATGGTAGATGGAAATTGACTCTTCTTAACTCTTCTTGGTGTTCTCATCAATGCCTTGTGCTAATTATTCTATTCAAGGCATTTACTTGTTTAAAACTTTTTATTGTCCTTTTAAGTTTTTTTCGTCTCTTTATCATATTAATCTTCTATGCTTTCTTTGTATCATGTATTTTTTTAGCATCACTCTCTTTTTTATTTGATCAATGAAAAACCTTGTTTCCACTTAAAAAGAAAAAGAGGCAAAGTTTGGATTCCTCTCTAAAACTTTTTCTTTTCTGTTTTCATCTCCCACACCTCCCAAGCCACGATGAAAGAAAGAACTTACCTTCGCATTTTGGTTACAGGAGTACTCCCCAACGACGCTGGTGATACTTATTACTCCTCCTCCAGTTGATGAGGAAGGGCGTAACGAATATGCACGGTATGACTTTTTCTTTCTAATTGCTTCTCAAAAAATTTTAGCCTTTCTTCTGTTGTAATGTTACCTACAACCGTCTATGTTTTCCATCTTCCTACTCCTTCAACTAGTTCCATCTTCCCACTTTTCGTTCAACAAGTTTTCTTACTGACGTCAGTTATATCAAAGCTCTACCAAAATATACTTTCTCATTCTCTTAACTATTGTGTGAATGTATCCTCATTGGTTTAGTCCAAATGTACTCCATGTCCTTGTTATACCCTTGTTGCATATTGTCGCCAATACCAACATTTAAAGGGTCTAAGAAGTAAGAACCCTACCGTTAGGGTGGAATGAACATGATTAGCCTAGCCTTTTACATGTGGTTCGTTCCAGAAATTCCATGCCTTTGTCGTCTCTGAGATACTTTAAGTTCAAAGTTCCATCAATATACTGAAGAGAGATTCTAACCCTACTCAGACTAAGGTGAAAATAATCTTGTATTGTTTCAGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCAGAGAGAACAAATGAAGTAACTGGACTTTATGCAAAAGAATGCCTTGAATTAGCTAAGGAAATAGGTCTTCATGCCATCGATTTATGGTCCAAGATGCAAGAAACGGAGGGTTGGCAGAAAAAGTTTCTCAGGTCTGTTATTCAAGCTCTTAATTCACAAGTTTTACTCATTTTATAAAATATGGCTGTGATCTATAATGTTATATGTCTTCAGTTGTCAGCATCCTCAGACCTGTGTGGAATGATTTTTTTAGTACTTAGAAAGTTATTCCCAATAGACTTTTAGTGTCGTAAATGAAATTGACATTATGACCTATTAGAGGGAGATGGATTGTTAATGAATATAGTAAATGATTCAATTCATCATAAATTAAATGCTGTACTTTTCTGAGTTTCTGATAGGTTGTTTCTTTTTTATCTTGTCTTATTGCAGGGATGGATTGCATTTCACACCAGAAGGCAATGGAGTTTTGCACCAAGAATTAGAGAAAGTACTCAATGAAACATCGGTTGCTGCTGCAAAAATGCCTCTTGATTTCCCACATCACTCAAAGATTGATGGCAAAAACCCCGAAAAGGCCTTTCAATATCTTTCTTTGTGATACACTCATCGTCTTTGTATCATGGTATATGATCATTGTTCTCCCTTCCCTTGTAATTTAAAGTTCCCCATTTGCATTTTGAGTGTTGACCATAATCATTTGTGTTTTATTTCAGTTTGTTTGATATGTATGAAAATTAAATATATGAGAAAGTGAAAATACTCGGAGA

mRNA sequence

GTTAAACATTTTCGAGTAGAATAAAGTGATAGAGAGGGGTCTCTCACTCATGAACATGAATATTTATTTTAATTATTTTCACATTTTAAACGTGGGCTGATCGGGTTTCTGGGATCTTCTTCTCCCATCTTAGAAAATATCTCAACCACTCTCCCTCTATCAATCTCCAGCCTGACGCCCTTCGCCTCACCTCAGACTAGTTGTCGCCGCACGTCCTGCTTCCGGTGTCGTCCTCCCGCTGCCGCCGCTCGCCACATTTTCAGTAGTGGGGTTGATTGAAGCGTTGAAATAACATACAATGAGGCCTGAGATCGTTCTGTTTGGGGATTCTATAACTGCACAATCCTTTGGTTTAGGTGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGAAAGGCCGATGTCATAGTTCGTGGCTATGGCGGATACAATACTCGGTGGGCTTTATTTTTACTTCATCACATTTTTCCCTTGAATTCCCCAAAGGCCCCCATTGTCGTGACAATATTCTTTGGGGCGAATGATGCAGCTGTTTTGGGGAGAACAAGTGAGAAACAACACGTACCTCTTGAAGAGTATAAGTATAATCTTAAAAAAATGGTAAATCATTTGAAGGAGTACTCCCCAACGACGCTGGTGATACTTATTACTCCTCCTCCAGTTGATGAGGAAGGGCGTAACGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCAGAGAGAACAAATGAAGTAACTGGACTTTATGCAAAAGAATGCCTTGAATTAGCTAAGGAAATAGGTCTTCATGCCATCGATTTATGGTCCAAGATGCAAGAAACGGAGGGTTGGCAGAAAAAGTTTCTCAGGGATGGATTGCATTTCACACCAGAAGGCAATGGAGTTTTGCACCAAGAATTAGAGAAAGTACTCAATGAAACATCGGTTGCTGCTGCAAAAATGCCTCTTGATTTCCCACATCACTCAAAGATTGATGGCAAAAACCCCGAAAAGGCCTTTCAATATCTTTCTTTGTGATACACTCATCGTCTTTGTATCATGGTATATGATCATTGTTCTCCCTTCCCTTGTAATTTAAAGTTCCCCATTTGCATTTTGAGTGTTGACCATAATCATTTGTGTTTTATTTCAGTTTGTTTGATATGTATGAAAATTAAATATATGAGAAAGTGAAAATACTCGGAGA

Coding sequence (CDS)

ATGAGGCCTGAGATCGTTCTGTTTGGGGATTCTATAACTGCACAATCCTTTGGTTTAGGTGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGAAAGGCCGATGTCATAGTTCGTGGCTATGGCGGATACAATACTCGGTGGGCTTTATTTTTACTTCATCACATTTTTCCCTTGAATTCCCCAAAGGCCCCCATTGTCGTGACAATATTCTTTGGGGCGAATGATGCAGCTGTTTTGGGGAGAACAAGTGAGAAACAACACGTACCTCTTGAAGAGTATAAGTATAATCTTAAAAAAATGGTAAATCATTTGAAGGAGTACTCCCCAACGACGCTGGTGATACTTATTACTCCTCCTCCAGTTGATGAGGAAGGGCGTAACGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCAGAGAGAACAAATGAAGTAACTGGACTTTATGCAAAAGAATGCCTTGAATTAGCTAAGGAAATAGGTCTTCATGCCATCGATTTATGGTCCAAGATGCAAGAAACGGAGGGTTGGCAGAAAAAGTTTCTCAGGGATGGATTGCATTTCACACCAGAAGGCAATGGAGTTTTGCACCAAGAATTAGAGAAAGTACTCAATGAAACATCGGTTGCTGCTGCAAAAATGCCTCTTGATTTCCCACATCACTCAAAGATTGATGGCAAAAACCCCGAAAAGGCCTTTCAATATCTTTCTTTGTGA

Protein sequence

MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLNSPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPPVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEGWQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYLSL*
Homology
BLAST of CSPI04G01360 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 365.5 bits (937), Expect = 4.5e-100
Identity = 164/238 (68.91%), Postives = 196/238 (82.35%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSF  GGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           S   P+  TIFFGANDAA+ GRTS++QHVP+EEY  N++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           P+DE GR  YA S+YG+KA + PERTNE TG+YA+ C+ LA+E+GL  ++LWSKMQET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQ 239
           WQKK+L DGLH TPEGNGV+  E+ +V  E  ++  +MP DFPHHS IDGKNP KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of CSPI04G01360 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 8.9e-64
Identity = 122/240 (50.83%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDSIT +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --NSPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILI 120
             +   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQE 180
           TPPP+DEE R  Y    Y +    LPERTNEV GLYAK C+ +A+E  +   DLWSKMQ+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAF 238
              WQ + L DGLH +  GN V+ +E+ K L E  + A  + +D P    +D K+P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of CSPI04G01360 vs. ExPASy Swiss-Prot
Match: Q2TAA2 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 6.0e-44
Identity = 103/234 (44.02%), Postives = 137/234 (58.55%), Query Frame = 0

Query: 3   PEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL-NS 62
           P ++LFGDSIT  SF  GGWGA+LAD   RK DV+ RG+ GYNTRWA  +L  +    NS
Sbjct: 16  PRLLLFGDSITQFSFQQGGWGASLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRKGNS 75

Query: 63  PKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYS-PTTLVILITPP 122
              P+ VTIFFGAND+A L   + KQH+PLEEY  NLK MV +LK    P   VILITP 
Sbjct: 76  LDIPVAVTIFFGANDSA-LKDENPKQHIPLEEYAANLKSMVQYLKSVDIPENRVILITPT 135

Query: 123 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 182
           P+ E    E    + G K      R N V G YA  CL++A++ G   +DLW+ MQ+++ 
Sbjct: 136 PLCETAWEEQC-IIQGCKL----NRLNSVVGEYANACLQVAQDCGTDVLDLWTLMQDSQD 195

Query: 183 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPE 235
           +   +L DGLH +P+GN  L   L  ++ +     + +PL  P+   +    PE
Sbjct: 196 F-SSYLSDGLHLSPKGNEFLFSHLWPLIEK---KVSSLPLLLPYWRDVAEAKPE 239

BLAST of CSPI04G01360 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-43
Identity = 90/227 (39.65%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           +RP+IVLFG SI   SF   GWGA LAD YSR AD+I+RGY G+N+R+AL +LH +FP +
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           +   P +V ++FG ND+     +    HVPL E+  N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           P++E    +    ++GD    +  R+NE+   YA+E L L +EI +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLN----ETSVAAAKMPLDFP 224
           W      DG+HFT + + ++ +E+ KVL     + S+    +P++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of CSPI04G01360 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 7.4e-42
Identity = 99/234 (42.31%), Postives = 136/234 (58.12%), Query Frame = 0

Query: 3   PEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLNSP 62
           P ++LFGDSIT  SF  GGWGA+LAD   RK DV+ RG+ GYNTRWA  +L  +    S 
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRKGSG 75

Query: 63  -KAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYS-PTTLVILITPP 122
             +P+ VTIFFGAND+A L   + KQHVPLEE+  NL+ MV +L+    P   +ILITPP
Sbjct: 76  LDSPVAVTIFFGANDSA-LKDENPKQHVPLEEFVANLRSMVRYLRSVDVPEGRLILITPP 135

Query: 123 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 182
           P+ E     +A+       +    R N V G YA+ CL++A++ G  A+DLWS MQ+   
Sbjct: 136 PLCEAA---WAQECLQQGCK--LNRLNSVVGEYARACLQVAQDCGADALDLWSLMQKDGQ 195

Query: 183 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPE 235
               +L DGLH +P+GN  +   L  ++ +     + +P   P+   I    PE
Sbjct: 196 DFSSYLSDGLHLSPKGNEFVFSHLWPLIEK---KVSSLPFLLPYWRDIAEARPE 240

BLAST of CSPI04G01360 vs. ExPASy TrEMBL
Match: A0A0A0KTH4 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.1e-139
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. ExPASy TrEMBL
Match: A0A1S3BZF4 (GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494719 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 9.1e-136
Identity = 233/242 (96.28%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPLN
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           SPKAPIVVTIFFGANDAA+LGRTSE+QHVPLEEYKYNLKKMVNHLKEYSPTTLV+LITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVNHLKEYSPTTLVVLITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAK+CLELAKEIGLH+IDLWSKMQET+G
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKKCLELAKEIGLHSIDLWSKMQETDG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGVLHQELEKVL ETSVAA KMPLDFPHHSKIDGKNPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLKETSVAAEKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. ExPASy TrEMBL
Match: A0A6J1E910 (GDSL esterase/lipase At5g62930 OS=Cucurbita moschata OX=3662 GN=LOC111430496 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.1e-128
Identity = 220/242 (90.91%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGN +LHQELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. ExPASy TrEMBL
Match: A0A1S3BYB4 (GDSL esterase/lipase At5g62930 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494719 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.3e-128
Identity = 233/298 (78.19%), Postives = 240/298 (80.54%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL- 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  -------------------------------------------------------NSPKA 120
                                                                  NSPKA
Sbjct: 61  PDLLEHCIIGSLDTLDAGFSGPKPICLHQNAPLLEAQFTNHPSCSRSFQPCFHPKNSPKA 120

Query: 121 PIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPPVDE 180
           PIVVTIFFGANDAA+LGRTSE+QHVPLEEYKYNLKKMVNHLKEYSPTTLV+LITPPPVDE
Sbjct: 121 PIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVNHLKEYSPTTLVVLITPPPVDE 180

Query: 181 EGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEGWQKK 240
           EGRNEYARSLYGDKARELPERTNEVTGLYAK+CLELAKEIGLH+IDLWSKMQET+GWQKK
Sbjct: 181 EGRNEYARSLYGDKARELPERTNEVTGLYAKKCLELAKEIGLHSIDLWSKMQETDGWQKK 240

Query: 241 FLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYLSL 243
           FLRDGLHFTPEGNGVLHQELEKVL ETSVAA KMPLDFPHHSKIDGKNPEKAFQYLSL
Sbjct: 241 FLRDGLHFTPEGNGVLHQELEKVLKETSVAAEKMPLDFPHHSKIDGKNPEKAFQYLSL 298

BLAST of CSPI04G01360 vs. ExPASy TrEMBL
Match: A0A6J1J450 (GDSL esterase/lipase At5g62930 OS=Cucurbita maxima OX=3661 GN=LOC111483251 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.3e-128
Identity = 219/242 (90.50%), Postives = 235/242 (97.11%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLIAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNG+LH ELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAF YL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHHELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFHYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. NCBI nr
Match: XP_011652964.1 (GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >XP_011652966.1 GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >KGN52868.1 hypothetical protein Csa_014576 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.1e-138
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. NCBI nr
Match: XP_008454268.1 (PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis melo] >XP_008454269.1 PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis melo])

HSP 1 Score: 492.7 bits (1267), Expect = 1.9e-135
Identity = 233/242 (96.28%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPLN
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           SPKAPIVVTIFFGANDAA+LGRTSE+QHVPLEEYKYNLKKMVNHLKEYSPTTLV+LITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVNHLKEYSPTTLVVLITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAK+CLELAKEIGLH+IDLWSKMQET+G
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKKCLELAKEIGLHSIDLWSKMQETDG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGVLHQELEKVL ETSVAA KMPLDFPHHSKIDGKNPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLKETSVAAEKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. NCBI nr
Match: XP_038904756.1 (GDSL esterase/lipase At5g62930 isoform X2 [Benincasa hispida])

HSP 1 Score: 482.3 bits (1240), Expect = 2.5e-132
Identity = 227/242 (93.80%), Postives = 239/242 (98.76%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPLN
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           +PKAPIVVTIFFGANDAA+LGRTSE+QHVPLEEYK NLKKMVNHLKEYSPT+LVILITPP
Sbjct: 61  TPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKDNLKKMVNHLKEYSPTSLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAK+CLELAKE+GLH IDLWSK+QETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKQCLELAKEMGLHVIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSV+A+KMPLDFPHHSKIDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVSASKMPLDFPHHSKIDGKHPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. NCBI nr
Match: XP_011652962.1 (GDSL esterase/lipase At5g62930 isoform X1 [Cucumis sativus] >XP_031740056.1 GDSL esterase/lipase At5g62930 isoform X1 [Cucumis sativus] >XP_031740057.1 GDSL esterase/lipase At5g62930 isoform X1 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 6.2e-131
Identity = 242/298 (81.21%), Postives = 242/298 (81.21%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL- 60
           MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  -------------------------------------------------------NSPKA 120
                                                                  NSPKA
Sbjct: 61  SDLLEHCIIGSLDTLDAGFSGPESICLHQNASLLEAQFTRHPSHSRSFQPCFHPKNSPKA 120

Query: 121 PIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPPVDE 180
           PIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPPVDE
Sbjct: 121 PIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPPVDE 180

Query: 181 EGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEGWQKK 240
           EGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEGWQKK
Sbjct: 181 EGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEGWQKK 240

Query: 241 FLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYLSL 243
           FLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYLSL
Sbjct: 241 FLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYLSL 298

BLAST of CSPI04G01360 vs. NCBI nr
Match: XP_023551925.1 (GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo] >KAG6576884.1 GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470.7 bits (1210), Expect = 7.6e-129
Identity = 221/242 (91.32%), Postives = 237/242 (97.93%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240
           WQKKFLRDGLHFTPEGNG+LHQELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CSPI04G01360 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 3.2e-101
Identity = 164/238 (68.91%), Postives = 196/238 (82.35%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           MRPEIVLFGDSITAQSF  GGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           S   P+  TIFFGANDAA+ GRTS++QHVP+EEY  N++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           P+DE GR  YA S+YG+KA + PERTNE TG+YA+ C+ LA+E+GL  ++LWSKMQET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQ 239
           WQKK+L DGLH TPEGNGV+  E+ +V  E  ++  +MP DFPHHS IDGKNP KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of CSPI04G01360 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 6.3e-65
Identity = 122/240 (50.83%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDSIT +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --NSPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILI 120
             +   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQE 180
           TPPP+DEE R  Y    Y +    LPERTNEV GLYAK C+ +A+E  +   DLWSKMQ+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAF 238
              WQ + L DGLH +  GN V+ +E+ K L E  + A  + +D P    +D K+P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of CSPI04G01360 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-44
Identity = 90/227 (39.65%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60
           +RP+IVLFG SI   SF   GWGA LAD YSR AD+I+RGY G+N+R+AL +LH +FP +
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120
           +   P +V ++FG ND+     +    HVPL E+  N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180
           P++E    +    ++GD    +  R+NE+   YA+E L L +EI +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLN----ETSVAAAKMPLDFP 224
           W      DG+HFT + + ++ +E+ KVL     + S+    +P++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of CSPI04G01360 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 1.5e-42
Identity = 87/236 (36.86%), Postives = 137/236 (58.05%), Query Frame = 0

Query: 2   RPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLNS 61
           RP+IVLFG SI   SFG GGWGA L++ Y+RKAD+I+RGY G+N+  AL ++  +FP ++
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPKDA 65

Query: 62  PKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPPP 121
              P +V ++FG ND+     +    HVPL EY  N+KK+  HL+  S  T +I ++ PP
Sbjct: 66  AVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIFLSSPP 125

Query: 122 VDE----EGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQE 181
           VDE    + ++ Y   +          RTN++   Y+  C+EL +E+GL  +DL+S  Q+
Sbjct: 126 VDEAKVRQNQSPYLSEVI---------RTNDLCKTYSDACVELCQELGLEVVDLFSTFQK 185

Query: 182 TEGWQKKFLRDGLHFTPEGNGVLHQELEKVLNET----SVAAAKMPLDFPHHSKID 230
            + W+     DG+H + +G+ ++  E+ +V+ E     S+    MP +F   S  D
Sbjct: 186 ADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYD 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FM044.5e-10068.91GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
Q6NMR98.9e-6450.83GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
Q2TAA26.0e-4444.02Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 P... [more]
O804431.8e-4339.65GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2... [more]
Q3SZ167.4e-4242.31Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KTH45.1e-139100.00SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 ... [more]
A0A1S3BZF49.1e-13696.28GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349471... [more]
A0A6J1E9104.1e-12890.91GDSL esterase/lipase At5g62930 OS=Cucurbita moschata OX=3662 GN=LOC111430496 PE=... [more]
A0A1S3BYB45.3e-12878.19GDSL esterase/lipase At5g62930 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349471... [more]
A0A6J1J4505.3e-12890.50GDSL esterase/lipase At5g62930 OS=Cucurbita maxima OX=3661 GN=LOC111483251 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_011652964.11.1e-138100.00GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >XP_011652966.1 GDSL... [more]
XP_008454268.11.9e-13596.28PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis melo] >XP_00845426... [more]
XP_038904756.12.5e-13293.80GDSL esterase/lipase At5g62930 isoform X2 [Benincasa hispida][more]
XP_011652962.16.2e-13181.21GDSL esterase/lipase At5g62930 isoform X1 [Cucumis sativus] >XP_031740056.1 GDSL... [more]
XP_023551925.17.6e-12991.32GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo] >KAG6576884.1 GDSL e... [more]
Match NameE-valueIdentityDescription
AT5G62930.13.2e-10168.91SGNH hydrolase-type esterase superfamily protein [more]
AT5G45920.16.3e-6550.83SGNH hydrolase-type esterase superfamily protein [more]
AT2G38180.11.2e-4439.65SGNH hydrolase-type esterase superfamily protein [more]
AT3G11210.11.5e-4236.86SGNH hydrolase-type esterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013830SGNH hydrolase-type esterase domainPFAMPF13472Lipase_GDSL_2coord: 7..197
e-value: 2.1E-26
score: 93.5
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 2..236
e-value: 5.8E-73
score: 247.1
IPR045136Isoamyl acetate-hydrolyzing esterase Iah1-likePANTHERPTHR14209ISOAMYL ACETATE-HYDROLYZING ESTERASE 1coord: 1..239
NoneNo IPR availablePANTHERPTHR14209:SF19GDSL-LIKE LIPASE/ACYLHYDROLASE FAMILY PROTEIN, EXPRESSEDcoord: 1..239
NoneNo IPR availableCDDcd01838Isoamyl_acetate_hydrolase_likecoord: 5..208
e-value: 5.02315E-99
score: 284.915
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 3..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G01360.1CSPI04G01360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds