CSPI04G01310 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G01310
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPMEI domain-containing protein
LocationChr4: 725818 .. 727087 (-)
RNA-Seq ExpressionCSPI04G01310
SyntenyCSPI04G01310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTTGTTCATCTTCTACAAAGCCCATCTCTTCCATCACCATTTCCCTCCATAATCTCATTCATCTCCCCTTTTCCCTTTAACCATTCTCATCTTCTCTTGTTCTCTGGAGGAAGAGAACAGCAGAATAAAAATGGAGCTCATCAATAATAGTATCCATAATGTCTTCCTCCTCCTCGCTTTCTCTTCTGTTTTCTCCTTTGCCGTTGCCGTTTGTGTCCCTCGCAACACCTCCGACATTGGATCCACTGCCATTCCTCCACTTTTTGAACAAACCGTTTCCCCTCAAGTTGCCCCTCAAGTTGCCCCTCAGTCACCGCCTGCCGTTACCGTTTCGGAGGAACCAGAGGCAGCCTCACCAGAAACATCGCCTTCTCAACCTTCCTCAGCTTCCGAATCTGAAGAAGCAGCTTCCGAAACTGAAGAAGACGACTTAGGACTACTTGGTGGAGGTTTGGACCAATTGCATTCGGCTGATTTGATATCGTCGGTAACATCGAGTGTGACGACGAGTCTGGTGGACATTTGCGATGTGACGAGCAATCCTCAACTATGTAAAACTTCGATTTCATCTCACATCGAGGGAACGACGGTGGATCCAGCTTCAGCACTGAAAACAGAGATTGATGAATCGATTAAAGAGGTAGCGAAAGCAATTGCAACATTGAATTCACTTCGTAAAGATTCAGCAGCATCAGAGACAGAAATCGCTTGCTACGATACTTGCCTGGAGAATTTCGAAATGGCTATTGATGATCTAAAAGCTGGAGTGGAATCGATAAATGCACGCGATGCTGGTAGAATGGAGAGTGTTCTAACGGCTGTAATGACAGACCTGACGACTTGCGATGATACTTTTGCAGAGATGGGAGTGGATTCTCCGTTGGATAGTCTTAGCACGAAGATGAGCAAATACGCCAGTAACTGCTTGGCCATTTCGAAATTGCTTCTGTAAAAACTGACTGAACCAGAGGCGAGTTGGAGAAGATGACTATTCCATTGATGGATGATTATATATGTCAATTCTTATTCTTATTATTGTTCTTCGTCTTCTTCAACTCATCAGAGTGTTCATATGGAGAAGACTGATCGAAAATACTCTCCGATGAACAAATTTTGTAAAGTGTTTTCGATGGCAAATGGGCAATGACAGTATTTTATGACCAAAAAAATAATATCTTAATTTATTATTCTTGTTTCATTTTTATCATTACTTGTATTCAAATTTTTATATTCTATCAATAACAAACTAAATATTGGAAGTCATTCA

mRNA sequence

GTTGTTGTTCATCTTCTACAAAGCCCATCTCTTCCATCACCATTTCCCTCCATAATCTCATTCATCTCCCCTTTTCCCTTTAACCATTCTCATCTTCTCTTGTTCTCTGGAGGAAGAGAACAGCAGAATAAAAATGGAGCTCATCAATAATAGTATCCATAATGTCTTCCTCCTCCTCGCTTTCTCTTCTGTTTTCTCCTTTGCCGTTGCCGTTTGTGTCCCTCGCAACACCTCCGACATTGGATCCACTGCCATTCCTCCACTTTTTGAACAAACCGTTTCCCCTCAAGTTGCCCCTCAAGTTGCCCCTCAGTCACCGCCTGCCGTTACCGTTTCGGAGGAACCAGAGGCAGCCTCACCAGAAACATCGCCTTCTCAACCTTCCTCAGCTTCCGAATCTGAAGAAGCAGCTTCCGAAACTGAAGAAGACGACTTAGGACTACTTGGTGGAGGTTTGGACCAATTGCATTCGGCTGATTTGATATCGTCGGTAACATCGAGTGTGACGACGAGTCTGGTGGACATTTGCGATGTGACGAGCAATCCTCAACTATGTAAAACTTCGATTTCATCTCACATCGAGGGAACGACGGTGGATCCAGCTTCAGCACTGAAAACAGAGATTGATGAATCGATTAAAGAGGTAGCGAAAGCAATTGCAACATTGAATTCACTTCGTAAAGATTCAGCAGCATCAGAGACAGAAATCGCTTGCTACGATACTTGCCTGGAGAATTTCGAAATGGCTATTGATGATCTAAAAGCTGGAGTGGAATCGATAAATGCACGCGATGCTGGTAGAATGGAGAGTGTTCTAACGGCTGTAATGACAGACCTGACGACTTGCGATGATACTTTTGCAGAGATGGGAGTGGATTCTCCGTTGGATAGTCTTAGCACGAAGATGAGCAAATACGCCAGTAACTGCTTGGCCATTTCGAAATTGCTTCTGTAAAAACTGACTGAACCAGAGGCGAGTTGGAGAAGATGACTATTCCATTGATGGATGATTATATATGTCAATTCTTATTCTTATTATTGTTCTTCGTCTTCTTCAACTCATCAGAGTGTTCATATGGAGAAGACTGATCGAAAATACTCTCCGATGAACAAATTTTGTAAAGTGTTTTCGATGGCAAATGGGCAATGACAGTATTTTATGACCAAAAAAATAATATCTTAATTTATTATTCTTGTTTCATTTTTATCATTACTTGTATTCAAATTTTTATATTCTATCAATAACAAACTAAATATTGGAAGTCATTCA

Coding sequence (CDS)

ATGGAGCTCATCAATAATAGTATCCATAATGTCTTCCTCCTCCTCGCTTTCTCTTCTGTTTTCTCCTTTGCCGTTGCCGTTTGTGTCCCTCGCAACACCTCCGACATTGGATCCACTGCCATTCCTCCACTTTTTGAACAAACCGTTTCCCCTCAAGTTGCCCCTCAAGTTGCCCCTCAGTCACCGCCTGCCGTTACCGTTTCGGAGGAACCAGAGGCAGCCTCACCAGAAACATCGCCTTCTCAACCTTCCTCAGCTTCCGAATCTGAAGAAGCAGCTTCCGAAACTGAAGAAGACGACTTAGGACTACTTGGTGGAGGTTTGGACCAATTGCATTCGGCTGATTTGATATCGTCGGTAACATCGAGTGTGACGACGAGTCTGGTGGACATTTGCGATGTGACGAGCAATCCTCAACTATGTAAAACTTCGATTTCATCTCACATCGAGGGAACGACGGTGGATCCAGCTTCAGCACTGAAAACAGAGATTGATGAATCGATTAAAGAGGTAGCGAAAGCAATTGCAACATTGAATTCACTTCGTAAAGATTCAGCAGCATCAGAGACAGAAATCGCTTGCTACGATACTTGCCTGGAGAATTTCGAAATGGCTATTGATGATCTAAAAGCTGGAGTGGAATCGATAAATGCACGCGATGCTGGTAGAATGGAGAGTGTTCTAACGGCTGTAATGACAGACCTGACGACTTGCGATGATACTTTTGCAGAGATGGGAGTGGATTCTCCGTTGGATAGTCTTAGCACGAAGATGAGCAAATACGCCAGTAACTGCTTGGCCATTTCGAAATTGCTTCTGTAA

Protein sequence

MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQSPPAVTVSEEPEAASPETSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL*
Homology
BLAST of CSPI04G01310 vs. ExPASy TrEMBL
Match: A0A0A0KWP8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.3e-134
Identity = 272/278 (97.84%), Postives = 272/278 (97.84%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60

Query: 61  SPPAVTVSEEPEAASPETSPSQPSSAS-----ESEEAASETEEDDLGLLGGGLDQLHSAD 120
           SPPAVTVSEEPEAASPETSPSQPSSAS     ESEEAASETEEDDLGLLGGGLDQLHSAD
Sbjct: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAESEEAASETEEDDLGLLGGGLDQLHSAD 120

Query: 121 LISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAI 180
           LISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGT VDPASALKTEIDESIKEVAKAI
Sbjct: 121 LISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDESIKEVAKAI 180

Query: 181 ATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDL 240
           ATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDL
Sbjct: 181 ATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDL 240

Query: 241 TTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           TTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL
Sbjct: 241 TTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 278

BLAST of CSPI04G01310 vs. ExPASy TrEMBL
Match: A0A5A7TT72 (Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001500 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.8e-98
Identity = 215/276 (77.90%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPR--NTSDIGSTAIPPLFEQTVSPQVAPQVA 60
           MELINN+I+NVFLLLAFSSVF  A+A+CVP   N +D+ STAIPPL EQTVSPQVAP   
Sbjct: 1   MELINNNINNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQVAPD-- 60

Query: 61  PQSPPAV-TVSEEPEAASPETSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSADLI 120
            QSPPAV TVS+ PEA SP+TS S  SS++E       ++E++ GLL  GLD LHS  LI
Sbjct: 61  -QSPPAVTTVSQPPEATSPQTSTSDQSSSNEI------SDEENSGLL--GLDYLHSDGLI 120

Query: 121 SSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAIAT 180
           S+  S+V T+LV+ICDVTSNPQLCKTSISSHIEGT VDPASALKTEI+ESIKEV KAIAT
Sbjct: 121 STAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIAT 180

Query: 181 LNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDLTT 240
           LNSLRKDS+ASETEIACYDTCLENF+MAI+DLKAGVESI+ARDAGRMESVLTAV+TDLTT
Sbjct: 181 LNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTT 240

Query: 241 CDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           CDDTFAEMGVDSPLD+LSTKMSKYASNCLAISKLLL
Sbjct: 241 CDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of CSPI04G01310 vs. ExPASy TrEMBL
Match: A0A1S3BYC7 (pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.8e-98
Identity = 215/276 (77.90%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPR--NTSDIGSTAIPPLFEQTVSPQVAPQVA 60
           MELINN+I+NVFLLLAFSSVF  A+A+CVP   N +D+ STAIPPL EQTVSPQVAP   
Sbjct: 1   MELINNNINNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQVAPD-- 60

Query: 61  PQSPPAV-TVSEEPEAASPETSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSADLI 120
            QSPPAV TVS+ PEA SP+TS S  SS++E       ++E++ GLL  GLD LHS  LI
Sbjct: 61  -QSPPAVTTVSQPPEATSPQTSTSDQSSSNEI------SDEENSGLL--GLDYLHSDGLI 120

Query: 121 SSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAIAT 180
           S+  S+V T+LV+ICDVTSNPQLCKTSISSHIEGT VDPASALKTEI+ESIKEV KAIAT
Sbjct: 121 STAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIAT 180

Query: 181 LNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDLTT 240
           LNSLRKDS+ASETEIACYDTCLENF+MAI+DLKAGVESI+ARDAGRMESVLTAV+TDLTT
Sbjct: 181 LNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTT 240

Query: 241 CDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           CDDTFAEMGVDSPLD+LSTKMSKYASNCLAISKLLL
Sbjct: 241 CDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of CSPI04G01310 vs. ExPASy TrEMBL
Match: A0A6J1KAN1 (pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.1e-57
Identity = 154/291 (52.92%), Postives = 187/291 (64.26%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           MELIN    NVFLLLA SS+F  A  VCVPRN++  GS     L +  V+PQ +P  +  
Sbjct: 46  MELINGL--NVFLLLALSSLFFPANTVCVPRNSAPFGS-----LVDSFVAPQASPSQSSS 105

Query: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAA------------------SETEEDDLG 120
             P++++S  P A SP  S     ++ ES+  A                  SE  E    
Sbjct: 106 PQPSISISPSPPAQSPTDSEDSALNSQESDSDALSPSDMEFPFDSYSSSEKSEASEASEA 165

Query: 121 LLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKT 180
                          SS  S+V T L +ICDVTS+PQLC  SISSHI+ + V+P SALKT
Sbjct: 166 SSPQSPSSTPPTSASSSSDSTVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALKT 225

Query: 181 EIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAG 240
           EI+ESIKEV KA+  L  LRK  +AS+ EIACYDTCLE F+MAI DL++G+ESI+ +D G
Sbjct: 226 EIEESIKEVRKAVTKLKLLRK--SASKVEIACYDTCLETFDMAIFDLQSGLESIDTKDTG 285

Query: 241 RMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
            MESVL AVM+DLTTCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

BLAST of CSPI04G01310 vs. ExPASy TrEMBL
Match: A0A6J1GAW7 (pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 9.5e-57
Identity = 150/285 (52.63%), Postives = 186/285 (65.26%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           ME+IN    NVFLLLA SS+F  A  VCVPRN++  GS       +  ++PQ +P  +  
Sbjct: 46  MEIINGL--NVFLLLALSSLFFPANTVCVPRNSAPFGSPV-----DSLIAPQASPSQSSS 105

Query: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSA------ 120
             P+V+ S  P A SP  S     ++ ES   A    + +         +   A      
Sbjct: 106 PQPSVSTSPSPPAQSPTNSEDSALNSQESYSDALSPSDMEFPFDSYSSSEKSEASSPQSP 165

Query: 121 ------DLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESI 180
                    SS  S+V T L +ICDVTS+PQLC  SISSHI+ + V+P SALKTEI+ESI
Sbjct: 166 SSTPPTSASSSDDSTVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALKTEIEESI 225

Query: 181 KEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVL 240
           KEV KA+  L SLRK  +AS+ EI+CYDTCLE F+MAI DL++G+ESI+ +D G MESVL
Sbjct: 226 KEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIDTKDTGTMESVL 285

Query: 241 TAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
            AVM+DLTTCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 AAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 321

BLAST of CSPI04G01310 vs. NCBI nr
Match: XP_011654315.2 (uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetical protein Csa_015367 [Cucumis sativus])

HSP 1 Score: 485.7 bits (1249), Expect = 2.6e-133
Identity = 272/280 (97.14%), Postives = 272/280 (97.14%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60

Query: 61  SPPAVTVSEEPEAASPETSPSQPSS-------ASESEEAASETEEDDLGLLGGGLDQLHS 120
           SPPAVTVSEEPEAASPETSPSQPSS       ASESEEAASETEEDDLGLLGGGLDQLHS
Sbjct: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAASESEEAASETEEDDLGLLGGGLDQLHS 120

Query: 121 ADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAK 180
           ADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGT VDPASALKTEIDESIKEVAK
Sbjct: 121 ADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDESIKEVAK 180

Query: 181 AIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMT 240
           AIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMT
Sbjct: 181 AIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMT 240

Query: 241 DLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           DLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL
Sbjct: 241 DLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 280

BLAST of CSPI04G01310 vs. NCBI nr
Match: XP_008454280.1 (PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa] >TYK29546.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 367.9 bits (943), Expect = 7.9e-98
Identity = 215/276 (77.90%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPR--NTSDIGSTAIPPLFEQTVSPQVAPQVA 60
           MELINN+I+NVFLLLAFSSVF  A+A+CVP   N +D+ STAIPPL EQTVSPQVAP   
Sbjct: 1   MELINNNINNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQVAPD-- 60

Query: 61  PQSPPAV-TVSEEPEAASPETSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSADLI 120
            QSPPAV TVS+ PEA SP+TS S  SS++E       ++E++ GLL  GLD LHS  LI
Sbjct: 61  -QSPPAVTTVSQPPEATSPQTSTSDQSSSNEI------SDEENSGLL--GLDYLHSDGLI 120

Query: 121 SSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAIAT 180
           S+  S+V T+LV+ICDVTSNPQLCKTSISSHIEGT VDPASALKTEI+ESIKEV KAIAT
Sbjct: 121 STAASTVKTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIAT 180

Query: 181 LNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDLTT 240
           LNSLRKDS+ASETEIACYDTCLENF+MAI+DLKAGVESI+ARDAGRMESVLTAV+TDLTT
Sbjct: 181 LNSLRKDSSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTT 240

Query: 241 CDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           CDDTFAEMGVDSPLD+LSTKMSKYASNCLAISKLLL
Sbjct: 241 CDDTFAEMGVDSPLDNLSTKMSKYASNCLAISKLLL 265

BLAST of CSPI04G01310 vs. NCBI nr
Match: XP_038903716.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 296.6 bits (758), Expect = 2.2e-76
Identity = 185/292 (63.36%), Postives = 212/292 (72.60%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVA-- 60
           MELIN    NVFLLLAFSS+FS A+A+CVPRN     ST+IPP  E  ++PQ    V   
Sbjct: 1   MELIN----NVFLLLAFSSLFSSAIAICVPRNALGFVSTSIPPTIEPQIAPQTTESVTAS 60

Query: 61  -PQSPPA------VTVS-------EEPEA---ASPETSPSQPSSASESEEAASETEEDDL 120
            PQSP A      VTVS       + P A    SP  SPS  ++++ +EE      E+D 
Sbjct: 61  LPQSPSAQTPQVEVTVSPPQSPPTQAPPATVTVSPPQSPSPSTNSAPAEEEQESASEEDS 120

Query: 121 GLLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALK 180
           GL      +L S +L+SSV SS TT LV+ICDVTSNPQLCKTSISS I G TVDP SALK
Sbjct: 121 GL--PEFQKLPSDNLVSSVASSTTTDLVEICDVTSNPQLCKTSISSQIHGMTVDPTSALK 180

Query: 181 TEIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDA 240
           TEI +S+KEV KAIATL  LRKD +AS  EIACYDTCLENF++AI+DLKAG+ESI+  D 
Sbjct: 181 TEIQQSMKEVTKAIATLKELRKDPSASNVEIACYDTCLENFDIAIEDLKAGLESIDVHDV 240

Query: 241 GRMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
           GRMESVLTAV+TDL TCDDTFAEMGVDSPLDSLST+MSKYASNCLAISKLLL
Sbjct: 241 GRMESVLTAVLTDLMTCDDTFAEMGVDSPLDSLSTRMSKYASNCLAISKLLL 286

BLAST of CSPI04G01310 vs. NCBI nr
Match: XP_022998626.1 (pectinesterase inhibitor 10 [Cucurbita maxima])

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-57
Identity = 154/291 (52.92%), Postives = 187/291 (64.26%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           MELIN    NVFLLLA SS+F  A  VCVPRN++  GS     L +  V+PQ +P  +  
Sbjct: 46  MELINGL--NVFLLLALSSLFFPANTVCVPRNSAPFGS-----LVDSFVAPQASPSQSSS 105

Query: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAA------------------SETEEDDLG 120
             P++++S  P A SP  S     ++ ES+  A                  SE  E    
Sbjct: 106 PQPSISISPSPPAQSPTDSEDSALNSQESDSDALSPSDMEFPFDSYSSSEKSEASEASEA 165

Query: 121 LLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKT 180
                          SS  S+V T L +ICDVTS+PQLC  SISSHI+ + V+P SALKT
Sbjct: 166 SSPQSPSSTPPTSASSSSDSTVPTDLEEICDVTSDPQLCTKSISSHIDESEVNPTSALKT 225

Query: 181 EIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAG 240
           EI+ESIKEV KA+  L  LRK  +AS+ EIACYDTCLE F+MAI DL++G+ESI+ +D G
Sbjct: 226 EIEESIKEVRKAVTKLKLLRK--SASKVEIACYDTCLETFDMAIFDLQSGLESIDTKDTG 285

Query: 241 RMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
            MESVL AVM+DLTTCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

BLAST of CSPI04G01310 vs. NCBI nr
Match: KAG7036636.1 (hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 231.5 bits (589), Expect = 8.8e-57
Identity = 153/291 (52.58%), Postives = 184/291 (63.23%), Query Frame = 0

Query: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60
           ME+IN    NVFLLLA SS+F  A  VCVPRN++  GS       +  ++PQ +P  +  
Sbjct: 46  MEIINGL--NVFLLLALSSLFFPANTVCVPRNSAPFGSPV-----DSLIAPQASPSQSSS 105

Query: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAA------------------SETEEDDLG 120
             P+V+ S  P A SP  S     ++ ES   A                  SE  E    
Sbjct: 106 PQPSVSTSSSPPAQSPTDSEDSALNSQESYSGALSPSDMEFPFDSYSSSEKSEKSEASEA 165

Query: 121 LLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKT 180
                          SS  S+V T L +ICDVTSNPQLC  SISSHI+ + V+P SALKT
Sbjct: 166 SSPQSPSSTPPTSASSSDDSTVPTDLEEICDVTSNPQLCTKSISSHIDESEVNPTSALKT 225

Query: 181 EIDESIKEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAG 240
           EI+ESIKEV KA+  L SLRK  +AS+ EI+CYDTCLE F+MAI DL++G+ESI  +D G
Sbjct: 226 EIEESIKEVRKAVTKLKSLRK--SASKVEISCYDTCLETFDMAIYDLQSGLESIETKDTG 285

Query: 241 RMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 274
            MESVL AVM+DLTTCDDTF EMGVDSPLD+LS+KMSKYASNCLAISKLLL
Sbjct: 286 TMESVLAAVMSDLTTCDDTFIEMGVDSPLDNLSSKMSKYASNCLAISKLLL 327

BLAST of CSPI04G01310 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 74/256 (28.91%), Postives = 118/256 (46.09%), Query Frame = 0

Query: 23  FAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQV-----APQSPPAVTVSEEPEAASPE 82
           F+     P     I S A  P    T SP  +P+      +P++PP V +     AASPE
Sbjct: 33  FSTPAPAPEVMKPIASPANSPAEIYTDSPVASPEAPADSFSPKNPP-VEIDIYSPAASPE 92

Query: 83  TSPSQPSSASESEEAASETEEDDLGLLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSN 142
              + P + +ES  AA    +     +      L +  L+S         +  IC  T N
Sbjct: 93  APVASPEAPAESSSAAPSGIK-----VSSSPKSLLNPPLLS-------PEIKTICGKTDN 152

Query: 143 PQLCKTSISSHI-EGTTVDPASALKTEIDESIKEVAKAIATLNSLRKDSAASETEIACYD 202
           P LC++S+S  +      + +S L   I  SI     A+A +  +     AS+       
Sbjct: 153 PPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKV----DASD------- 212

Query: 203 TCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDLTTCDDTFAEMGVDSPLDSLST 262
            C E ++ A+ +L+  V ++ + D   + + L+A MTD +TC+D F E G  +PL  ++ 
Sbjct: 213 -CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVAD 263

Query: 263 KMSKYASNCLAISKLL 273
           K++K  SNCLAIS L+
Sbjct: 273 KLTKMVSNCLAISTLI 263

BLAST of CSPI04G01310 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-08
Identity = 44/157 (28.03%), Postives = 77/157 (49.04%), Query Frame = 0

Query: 116 LISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEVAKAI 175
           L ++ + +++ SL D C V+  P LC+++I      T V+ A+      D +IKE+ K  
Sbjct: 13  LAATSSQALSISLADPCTVSDFPALCRSTIKGQ---TNVNAAT------DVAIKELMKRT 72

Query: 176 ATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDL 235
                + +     +  +A   TCL NF  A D+L+  + +I   D   +   L+A +TD 
Sbjct: 73  RQAKKIAEKELKRDGGVA---TCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDY 132

Query: 236 TTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLL 273
            TC D+  E    + +   +  + + A NCLA+S L+
Sbjct: 133 DTCSDSMKETKEVNVIYKSAGVLYRMADNCLALSTLV 157

BLAST of CSPI04G01310 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.9e-05
Identity = 57/222 (25.68%), Postives = 101/222 (45.50%), Query Frame = 0

Query: 58  APQSPPAVTVSEEPEAA-SPETSPSQPSSASESEEAASETEEDDLGL--LGGGLDQLHSA 117
           +P S   +  S  P AA SP  +P+      E++ +A  T+ + L L  L      L   
Sbjct: 20  SPSSSTRIISSIVPSAAPSPAVAPTTDGDVDENDFSAF-TQWNILNLTDLKSTFKNLPDF 79

Query: 118 DLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEG---TTVDPASALKTEIDESIKEV 177
             ++  +  V+ ++  +C  T     C  SI   +         P   L+ E+    ++ 
Sbjct: 80  SKLNISSLHVSPAVGSVCSNTDYAAECIVSILPLLRDFRKFEPKPIDVLRMEMSALYEKA 139

Query: 178 AKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAV 237
              +     L  D +         D C++N+E  +DDLK    +++  D  R+ESV++A 
Sbjct: 140 NATLDLAKRLIVDKSTPRDVADVLDLCVDNYESLLDDLKDASVAVDDGDFERLESVVSAA 199

Query: 238 MTDLTTCDDTFAEMG-VDSPLDSLSTKMSKYASNCLAISKLL 273
           + D+ TC D FAE   ++SP+ ++   + K  SN LAIS+++
Sbjct: 200 IADVVTCSDAFAESSELESPMANVDDFLKKLCSNVLAISQMI 240

BLAST of CSPI04G01310 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 6.4e-05
Identity = 43/161 (26.71%), Postives = 69/161 (42.86%), Query Frame = 0

Query: 113 SADLISSVTSSVTTS-LVDICDVTSNPQLCKTSISSHIEGTTVDPASALKTEIDESIKEV 172
           +A LI  +   V  S +++IC  T+ P LC+  +          P  A    I     + 
Sbjct: 18  AAVLIFLMAGQVAESRMINICSHTAYPSLCRPLVK-----RVTSPRKATHRTIQALEAKT 77

Query: 173 AKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAV 232
             A+A     +  + A         TC E    A+ +L +  +SI  RD   M + LTA 
Sbjct: 78  KLALAETARFKNGNQA-------VSTCYETLGDAVYNLASARKSIRKRDVPAMNTYLTAA 137

Query: 233 MTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLL 273
           ++D   C D F E    + + +    + K +SNCLA+S L+
Sbjct: 138 VSDYGACVDGFIETQQVNAIQNAVVDLRKISSNCLALSTLI 166

BLAST of CSPI04G01310 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.1e-04
Identity = 69/248 (27.82%), Postives = 113/248 (45.56%), Query Frame = 0

Query: 38  STAIPPLFEQTVSPQVAPQVAPQSPPAVTVSEEPEAAS---PETSPSQPSSASESEEAAS 97
           S+  P L E  VS   +  V P S P+ T SE+    S   P  + S P  + E  +   
Sbjct: 41  SSPDPSLIEPPVS---SISVVPASAPSPT-SEDINTGSNLAPTAAVSAPGPSQEDIDINF 100

Query: 98  ETEEDDLGLLGGGLDQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSI----SSHIE 157
           ++   ++  L    + ++  D  S     + T+  D+C  T     C  +I        E
Sbjct: 101 DS-VTNIADLAPKFEHINKFDFSS---MKIDTTAKDLCKNTDYNNECIAAILPDLQKQAE 160

Query: 158 GTTVD----PASALKTEIDESIKEVAKAIATLNSLRKDSAASETEI---ACYDTCLENFE 217
           G   D    P   ++ E +  +K   KA ATL+  ++     +T +        C+EN++
Sbjct: 161 GEGGDGGIAPKDVMRMEAESLLK---KANATLDYAKRVVEDRKTPMPVKEAMSVCVENYD 220

Query: 218 MAIDDLKAGVESINARDAGRMESVLTAVMTDLTTCDDTFAEM-GVDSPLDSLSTKMSKYA 271
             I  L+    ++   D GR++SVL+A ++D++TC D F ++  VDSP  SL   M K  
Sbjct: 221 SLISGLEDAKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKKLC 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KWP87.3e-13497.84PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 S... [more]
A0A5A7TT723.8e-9877.90Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BYC73.8e-9877.90pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1[more]
A0A6J1KAN11.1e-5752.92pectinesterase inhibitor 10 OS=Cucurbita maxima OX=3661 GN=LOC111493211 PE=4 SV=... [more]
A0A6J1GAW79.5e-5752.63pectinesterase inhibitor 10 OS=Cucurbita moschata OX=3662 GN=LOC111452501 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_011654315.22.6e-13397.14uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetica... [more]
XP_008454280.17.9e-9877.90PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesteras... [more]
XP_038903716.12.2e-7663.3621 kDa protein-like [Benincasa hispida][more]
XP_022998626.12.3e-5752.92pectinesterase inhibitor 10 [Cucurbita maxima][more]
KAG7036636.18.8e-5752.58hypothetical protein SDJN02_00256, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G36659.11.1e-0928.91Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G23350.12.8e-0828.03Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G02550.12.9e-0525.68Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G10770.16.4e-0526.71Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT2G47340.11.1e-0427.82Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..219
NoneNo IPR availableCOILSCoilCoilcoord: 164..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..90
NoneNo IPR availablePANTHERPTHR31707:SF119INVERTASE-RELATEDcoord: 13..267
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 13..267
NoneNo IPR availableCDDcd15800PMEI-like_2coord: 130..272
e-value: 1.51388E-47
score: 153.67
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 123..268
e-value: 6.5E-24
score: 95.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 129..268
e-value: 3.8E-20
score: 72.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 124..272
e-value: 1.7E-24
score: 84.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 126..272
e-value: 5.6E-29
score: 103.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 130..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G01310.1CSPI04G01310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity